LOCUS       CP000033               15057 bp    DNA     linear   BCT 30-JAN-2014
DEFINITION  Lactobacillus acidophilus NCFM, complete genome.
ACCESSION   CP000033
VERSION     CP000033.3
DBLINK      BioProject: PRJNA82
            BioSample: SAMN02603047
KEYWORDS    .
SOURCE      Lactobacillus acidophilus NCFM
  ORGANISM  Lactobacillus acidophilus NCFM
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactobacillus.
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Complete genome sequence of the probiotic lactic acid bacterium
            Lactobacillus acidophilus NCFM
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (11), 3906-3912 (2005)
   PUBMED   15671160
REFERENCE   2  (bases 1 to 15057)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
REFERENCE   3  (bases 1 to 15057)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 15057)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
COMMENT     On Nov 1, 2007 this sequence version replaced CP000033.2.
FEATURES             Location/Qualifiers
     source          1..15057
                     /organism="Lactobacillus acidophilus NCFM"
                     /mol_type="genomic DNA"
                     /strain="NCFM"
                     /db_xref="taxon:272621"
     gene            complement(1..858)
                     /locus_tag="LBA1864"
     CDS             complement(1..858)
                     /locus_tag="LBA1864"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DT26|3.A.1.1.27"
                     /protein_id="AAV43664.1"
                     /translation="MKSYSNQRRLSLIFRYILLTVLAILWILPIVWIVLASFSYNDTGF
                     VSTFWPEQFTLQNYIGIFTNSQYPFVNWIINTLIVSLLSMIISTFLTISVAYVLSRLRF
                     KFRKPFLQIALVLGMFPGFMSMIALYYILKGLNMLNLFGLLLVYVGGAGLGFYVAKGFF
                     DTIPRSIDEAAEIDGATKWQVFLHIGLPLSKPMIVYTALTAFIAPWTDFIFSGIILSTS
                     GNAKTYTIAYGLYNMVHTAKGNATAYFAQFVAGCVIIAIPITILFVFMQKFYVNGITAG
                     ADKG"
     gene            complement(861..2234)
                     /locus_tag="LBA1865"
     CDS             complement(861..2219)
                     /locus_tag="LBA1865"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DT27|3.A.1.1.27"
                     /protein_id="AAV43665.1"
                     /translation="MFLKKKHVAPKATYREVWSKGDLATKLSFFIMGSNALANKQWVKG
                     LAFLLSEIIFIVWFVLSGIPTLNTLATLGTNKTKKVVYDAAQGVYVTKQPSNSVLILLF
                     GVLAVILCIAIIYLYIVNLRSTRHNYILKRDGGHIPTNVEELKSLLDTRLHATLMVIPL
                     LGILFFTILPTIFMISMAFTNYDRQHPIAFSWTGFQAFGNVLSGDLAGTFFPVLGWTLI
                     WAVAATATTFFFGVLLALLIESKGIKHKGFWRTVFVIVWAVPQFVSLLMMAQFLDYQGA
                     LNNILMNLHWISSPIHFIDNQASPLVARITVIIVNMWIGIPVSMLVSTAIIQNLPQDQI
                     EAAKIDGANAAQIFRSITFPQILFVMAPSLIQQFIGNINNFNVIFLLTGGAPMNSHYNG
                     AGSTDLLVTWLYNLTFGQEQRYNASAVLGILIFIISAVVSLIAYRHTNAYKEG"
     regulatory      complement(2227..2234)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1865"
     gene            complement(2290..3530)
                     /locus_tag="LBA1866"
     CDS             complement(2290..3516)
                     /locus_tag="LBA1866"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DT28|3.A.1.1.27"
                     /protein_id="AAV43666.1"
                     /translation="MKFWKKMALGSTAVLAAMSLAACSNGSSNSSSSSNTSNKKANLTL
                     WVDTEQVPYYKQIAKDFTKKNKNIKVRVVQSPNGSANAKTDVGKDPSKAADVFEVPNDQ
                     LGQMAEAGYINPLSPSASKDIKANYIDVASKGVTWKGKVYAFSYAQQAQTIYYNKSKLS
                     ANDVKDWKTLTSKGVVATDFTNAYVMWPVMFSAGTKLYGNNGEDLKGSTFNSQDGINAL
                     KWYAAQKKNKGVMQTSNALNQLKKGNAQAILDGPWNAANIKKILGKNFAVAKYPKIDVG
                     GKNVQMEAFLGIEGFAVNSNTKNAKAASDLAAYITNKKSQLIAHKEAGQIPVLKAAVNS
                     SAVKSDPVAEAVIEMAKPGNSVLQPKLPQMAAFWNGSAPLISGTYDGKIKPSQYKAQLA
                     KLAKTVEKK"
     regulatory      complement(3524..3530)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1866"
     gene            complement(3603..4709)
                     /gene="msmK"
                     /locus_tag="LBA1867"
     CDS             complement(3603..4709)
                     /gene="msmK"
                     /locus_tag="LBA1867"
                     /note="MsmK"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q7WWQ6|3.A.1.1.20"
                     /protein_id="AAV43667.1"
                     /translation="MVEVDLNHIYKKYAGNDRYSVNDFNLHIKDKEFIVFVGPSGCGKS
                     TTLRMVAGLEDISEGTLEIDHKVMNDVAPKDRHIAMVFQNYALYPHMTIYDNMAFGLKL
                     RHTPKDVIDKKVKEAAKMLGLEDYLDKKPGALSGGQRQRVALGRAIVRAAPIFLMDEPL
                     SNLDAKLRVVMRTEIAKLHQELGTTTIYVTHDQTEAMTLADRVVVMSVGKVEQIGSPLE
                     VYNKPVNMFVAGFIGSPQMNFFNVHYKNGRISDGKGLNIGIPEGKAKLLEEKGYNDKDL
                     VFGIRPEDIHSEEAFMETWPDSVVESKVVVSELLGATIQLHQEVDGTEFTAIVNSRDFH
                     KPGDMVRMGFDVNKAHFFDKDSTKAIVN"
     gene            4758..6140
                     /locus_tag="LBA1868"
     CDS             4758..6140
                     /locus_tag="LBA1868"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|HTH_12"
                     /protein_id="AAV43668.1"
                     /translation="MSKLNKKQRLKIYNDWRNGNESLSVIASEIKLNRSTLQYMVNLID
                     RHGIEIYDKSKELFTKEFKEKAIVRSLTGNNSINQLSLDLGLRSNGILYNWIREYKKNG
                     YNVVIKKKGRHSVHESEEAIPNIAQRFREADPATQRRKLEIAYRQCLRKKTKRLGSETS
                     QEIAQAVTELRQEFKVSLDYIFTVLAQNKDLPLIARSTYYNIIKPKDKPKRKKPGFLSR
                     MKEIFDYHQRRYGYRRVAMQLRKEGYAVTDRAVRYWMHKLKLKGRRRNKRKYSSYKGTI
                     GKIAPNLIHRGFFAIRPNMKWYTDITEFHLNGQKLYLSPILDGCGGDIVSYSISRHPDM
                     NLVMSMLNKAFAKHKALNACIFHTDQGCQYQSRVYQRALKLHGITQSMSRKGNSMDDGL
                     MENFFGLLKTEMFYDQEYKYHNLEELTQAIEEYIKYYNNERIKSRLKGLTPIEYRDQAL
                     VS"
     gene            complement(6198..6863)
                     /gene="pgmB"
                     /locus_tag="LBA1869"
     CDS             complement(6198..6863)
                     /gene="pgmB"
                     /locus_tag="LBA1869"
                     /EC_number="5.4.2.6"
                     /note="COG0637 Predicted phosphatase-phosphohexomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="AAV43669.1"
                     /translation="MLKGLIFDLDGVLTDSARFHLTAWNNLAKELGITLTNEQLDSLRG
                     ISRMDSLNLILKYGGQEDKYTEAEKEKFAAEKNAKFVEQVETMTPKDILPGIPELLSDA
                     KKQNLKMVIASASKNAPKILTRLGIMDEFDGIVDPATLHHGKPDPEIYIKAQELVGLKA
                     NEVISFEDAQAGVEAIKAAHQFAVGIGNKKLLKEADYIVPTTADLKLSEIEKVFEEKE"
     gene            complement(6848..9130)
                     /locus_tag="LBA1870"
     CDS             complement(6848..9118)
                     /locus_tag="LBA1870"
                     /EC_number="2.4.1.8"
                     /codon_start=1
                     /transl_table=11
                     /product="GH65"
                     /protein_id="AAV43670.1"
                     /translation="MKRIFEIDPWKVITHKFDPKDKRLQESMTAIGNDYMGMRGNFEEG
                     YSGDSLQGTYLAGVWFPDKTVVGWWKNGYPKYFGKTPNAPSFIGIGINVNGEKVDLAKV
                     KFSDFELSLDMHQGLLSRSFIYEGKDVKVKLEFERFLHIVQKEAALIKVKATVLEGHAK
                     IDFDSTLDGTVVNEDSNYGDRFWIPLGEDKDEKTIQVKTKKNPYDVPQFTVLLKEALRN
                     NGVAVNGEVTTEDAKLSERFSVELDEGQSYELEKDVIVVTSRDVEEKDQAAVANNLMSK
                     LQTKSFEDNLADHTEAWKKRWETSDVEISGDDAAQQGIRFNICQLFMTYYGEDKRLNVG
                     PKGFTGEKYGGATYWDTEAFIVPMYLAVTKPSVTRALLQYRHDQLPGAYHNAKEQGLPG
                     ALFPMVTFNGIECHNEWEITFEEIHRNADIPHAIAMYTDYTGDDSYVKNEGMDVLVGTA
                     RFWAARVHWSKMRNKYVMHGVTGPNEYENNVNNNWFTNTMARWLLKYTLERLPLATKEA
                     QERVRVTDEEKAKWQDIVDNMYLPEDEDLGIFLQQDDFLDKDIRPVTEIEDQRPINQHW
                     SWDKILRSPFIKQADVLQGIYFFDDQYTMDQKEKNFDFYEPLTVHESSLSPCIYSIMAA
                     ELGKKEKAVELYQRTARLDLDNYNNDTVDGLHITSMSGSWLAIVQGFAGMRYDHDQLKF
                     NPFVPDGWDHYSFKINYRGRLIEVYVDHDECKITLLSGDDLEVMVHDNKLDLKEGKTKC
                     LKA"
     regulatory      complement(9125..9130)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1870"
     gene            complement(9289..11010)
                     /locus_tag="LBA1871"
     CDS             complement(9289..11010)
                     /locus_tag="LBA1871"
                     /EC_number="3.2.1.135"
                     /note="COG0366 Glycosidases"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM34|GH13|GH13_20"
                     /protein_id="AAV43671.1"
                     /translation="MQLAALRHRTESEDSFVVDPKHVRVRFHTAKNDVEKVIVHYCDNY
                     LPLKFAETTEMEKIGEGQVEDYWGITLDAPYHRLKYTFEVIGKDGTSVVYGDRAISDDI
                     KDAINEDGSYFKIPYCHEIDMVKTPDWVKNTVWYQIFPERYANGDKSNDPKNIKPWNPT
                     DHPGREDYYGGDLQGVLDHLDYLQKLGVNGLYFCPIFKASSNHKYDTIDYLQVDPEFGD
                     KDLFAKVVNEAHARGMKIMLDAVFNHLGDQSMQWQDVVQNGSKSRFADWFHINEYPVEP
                     YRNPLKGEKNPQYDTFAFEEHMPKLNTANPEVQDFLLEIATYWVKYFDIDAWRLDVANE
                     VDHHFWKKFHKAVTDIKPDFYIVGEVWHSARPWLNGDEFTGVMNYPYTLQIEDHFFKHK
                     LTADQMTTRLTDQLMKYRDSTNEAMMNMLDSHDTARILTVAKGDQDLALQALTFEFMQK
                     GSPCIYYGTEMGMAGDNDPDCRKPMDWSKEDGPVWQRVHKLIKFRLAHDKTLSEGKIKL
                     SVTENRLIEVIREGKESIHAYFNTTKNDVKLDGKAELSQDFEKGILAPKGFVIMVD"
     gene            complement(11014..12683)
                     /locus_tag="LBA1872"
     CDS             complement(11014..12669)
                     /locus_tag="LBA1872"
                     /EC_number="3.2.1.10"
                     /note="COG0366 Glycosidases"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q05839|8.A.9.1.1"
                     /protein_id="AAV43672.1"
                     /translation="MSHWYDHAIIYQIYPKSFQDSNDDGIGDLNGIRKRIPYLQNLGVN
                     AVWLNPVFVSPQVDNGYDVSNYFAIDSHMGTMEDMENLIKDLHKAGIHIIMDFVLNHTS
                     DQHPWFQDAIKNPDSLYRDYYIFAGHDNKQPNNWGSFFGGSVWEPDPAGTGQSYFHLFD
                     KRMPDLNWKNPEVRHAMLEIAEFWLKKGIDGLRLDAFIHIGKADLRQNYPAMDDKPVIA
                     EPFFANLPQVQEWMRPFCEQIKEDYPDALLLGEAASASVNLAVDYTNKRNHLMDCVITF
                     RYFTEDDSKIDKSYSAQYQPKELDLTAFKQNQVVWQQTLADISQPTLYWNNHDMARLAT
                     RIAKTSTQAKSLAMLMYLQRGIPIIYYGEELGLKNLHFTSVDQFEDQTVAPWIKEAQKA
                     GISRDAAFAMVSDTHKLPARGPMPWNDTENNGFTSAKPWLNGISQDDVTVANEVNSDNS
                     MFTFYKNMLNLKKEKLFQDGTYYMISTGKDSYVYQRDLGNESAIVAVSLSNKKISIDLP
                     EEYIKELLKAGEYQLTNGKLTLMPYAGVVLKKEN"
     regulatory      complement(12677..12683)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1872"
     gene            complement(12781..13970)
                     /gene="ackA"
                     /locus_tag="LBA1873"
     CDS             complement(12781..13956)
                     /gene="ackA"
                     /locus_tag="LBA1873"
                     /EC_number="2.7.2.1"
                     /note="COG0282 Acetate kinase"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="AAV43673.1"
                     /translation="MKKVLAINSGSSSFKYKLFTFPNEKVVASGMADRVGMANAVFKIK
                     LNSKEYIKNTPIPNQEAAVSLLIESLKKFNVVKDLKELSGVGHRVVNGGEVFKHSVVIN
                     QANLKQLIKLGNLAPLHNIPETVGIEAFMQIIPDIPQIAVFDTSYHQSLSEINYLYSIP
                     YRYYEDYAVRKYGAHGISVEYVAPRAAKMMGKNLNISKLIVCHLGSGSSVTAVKNGKSY
                     DTSMGFSPLTGVTMGTRSGDFDPSALRYIMDKTGMSIDEGIDMLNHESGLLGISGVSSD
                     MRDLIESKQKRAELARRIYINRVVRYVGAYAAEMDGVDGIIFTAGVGEHDSGIRAGVMS
                     SLRYLGLKPDYKANRTDGEKFISMPNSKVKALVVPTNEELMIAREVVRLTR"
     regulatory      complement(13965..13970)
                     /regulatory_class="ribosome_binding_site"
                     /gene="ackA"
                     /locus_tag="LBA1873"
     gene            complement(14097..15057)
                     /locus_tag="LBA1874"
     CDS             complement(14097..15047)
                     /locus_tag="LBA1874"
                     /note="LacI; COG1609 transcriptional regulators"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="AAV43674.1"
                     /translation="MTTLSDVAKEANVSKMTVSRVINHPEQVTPELRAMVEKAMEKLNY
                     HPNSIAQALVNNRSNVVKFVTLEDIDTTEPYYTNLLFGFARGLNAKQYALQLVVDPTRI
                     EKGRADGYLITGARNEDYDMFDKLDKPFVLFGENHRGYDFVDTDNQTAEKMATQYALDR
                     LYKHIIFIGIDIKEPFEYSREAGYINTLQQHRLIPQIYRVSNHSHAAQKVIHEHFKEFK
                     KDTCFICASDRIAVGVVRQLQDERARIPEDFGVIGFDGVFLDQVSNPKLTTIKQPIFEM
                     GKMLAKMLLQKIDQSGSPQGELMVKPQLIRRETTR"
     regulatory      complement(15051..15057)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1874"
ORIGIN
        1 ttaaccttta tcagcaccag cagtaatacc attaacatag aacttctgca tgaagacaaa
       61 caagatagta atagggatag caatgattac acatcctgca acgaattgtg caaagtatgc
      121 agttgcatta cccttagccg tgtgaaccat gttgtaaagt ccgtaggcaa tagtataagt
      181 cttcgcattt cctgaagttg aaagaatgat acctgagaaa atgaagtctg tccaaggtgc
      241 aatgaaagca gttaaagcag tataaacgat cattggcttt gaaagtggca atccaatatg
      301 aagaaatact tgccacttag tagcaccatc aatttcagca gcttcatcaa ttgatctagg
      361 aattgtatca aagaatcctt tagcaacgta gaagccaagt ccggcaccac caacgtaaac
      421 caaaagtaaa ccaaagaggt ttaacatatt caaacctttt aagatgtagt acaaggcaat
      481 cattgacatg aaacctggga acatccccaa aactaaagca atttgaagaa atggctttct
      541 aaacttaaaa cgaagtcgtg ataaaacata ggcaactgaa attgttaaga atgttgagat
      601 aatcattgaa agaagtgaaa caatcaatgt attaataatc cagttaacaa atggatactg
      661 tgaattagta aaaataccaa tatagttttg caaagtaaat tgttctggcc agaaggtcga
      721 aacaaatcca gtatcattgt atgaaaaact tgccaagacg atccagacaa tcggtaaaat
      781 ccaaagaata gctaatacag ttaaaagaat ataccgaaag attaatgaga gccgtctttg
      841 attagaatat gacttcatgt ctagccctcc ttataagcgt tagtgtgtct atatgctatc
      901 aacgatacaa ccgcactaat tataaagatt aagataccaa gtacggctga agcgttatat
      961 cgctgctctt gcccgaacgt taagttatac aaccacgtta ccagtaagtc agtcgatccg
     1021 gcgccgttat agtgactgtt catcggcgca ccacctgtca gcaagaagat aacattgaag
     1081 ttgttaatgt taccaataaa ttgttggata agtgaaggtg ccataacaaa taagatttgt
     1141 gggaaagtaa ttgatctgaa aatttgtgca gcattagcac catcaatctt ggcagcttca
     1201 atttgatctt gtggcaagtt ttgaataata gctgtagata ccagcattga aactggaata
     1261 ccgatccaca tgttaacaat aataactgta attctggcta ccaatggtga agcctggtta
     1321 tcaataaagt ggataggaga gctaatccag tgtaagttca tcaagatatt gtttaaagca
     1381 ccttgatagt ccaagaactg agccatcatt aaaagtgaaa cgaattgagg aacagcccaa
     1441 acaattacaa agactgttct ccaaaagccc ttatgtttaa tacccttgga ttcaatcaat
     1501 aaagcaagta acacaccaaa gaagaaagta gtagcagtag ctgctacagc ccaaatcaat
     1561 gtccagccca atactgggaa gaaagtaccc gctaagtcac cactaagaac attaccaaat
     1621 gcttgaaatc cagtccaaga gaaggcaatt gggtgttgac ggtcatagtt agtaaatgcc
     1681 attgaaatca taaagattgt tggcaagatg gtaaagaaca aaatacctaa taatggtata
     1741 accattaaag ttgcgtgtaa acgcgtgtca agtaagctct taagttcttc aacattggtc
     1801 ggaatgtgac caccatcacg ttttaatatg taattatgtc ttgttgagcg caagttaaca
     1861 atgtatagat aaataattgc tatacataaa attactgcca gcaccccaaa tagcaaaatt
     1921 aaaactgagt ttgacggttg cttagtaaca taaacacctt gtgcagcatc ataaacaact
     1981 tttttggtct tattagtacc taatgtagct aaagtattta aagttggaat accgctaaga
     2041 acaaaccaaa caatgaaaat aatttctgaa agtaaaaatg caagaccttt tacccattgt
     2101 ttgttagcta aggcattact acccattatg aaaaatgata acttggtagc aagatcacct
     2161 tttgaccata cttcacggta tgtcgcttta ggagctacat gctttttctt tagaaacata
     2221 acttctcccc ttcttaggca aaaaggttgg acaattttta gcccaacctt tgcttatgct
     2281 tttagttgac tatttctttt caacagtctt tgcgagttta gcaagttgtg ccttgtattg
     2341 acttggctta atcttaccat cgtaagtacc actgataagt ggagctgaac cattccagaa
     2401 tgcagccatt tgtggaagct taggttgcaa tactgagtta cctggcttag ccatttcaat
     2461 tacagcttca gcaactggat cactcttaac tgcacttgag ttaactgcag cctttaatac
     2521 aggaatttga cctgcttctt tgtgagcaat caattgtgac ttcttattag tgatgtaagc
     2581 agctaagtct gaagcagctt tagcattctt agtgtttgag ttaactgcga agccttcaat
     2641 accaaggaat gcttccattt gtacattctt accgccaaca tcaatcttag gatacttagc
     2701 aactgcaaag ttcttaccta aaatcttctt aatattagct gcgttccatg gaccatcaag
     2761 gattgcttga gcattaccct tcttcaattg attcaaagca tttgaagttt gcattacgcc
     2821 tttattcttc ttttgagctg cgtaccactt caaagcatta ataccgtctt gtgaattaaa
     2881 tgttgaaccc ttaaggtctt caccattgtt accataaagc ttcgtacctg ctgagaacat
     2941 tactggccac attacatatg cgttagtgaa gtcagtagct acaacacctt tagaagttaa
     3001 agtcttccaa tccttaacgt catttgctga aagcttagac ttgttgtagt aaatagtttg
     3061 agcttgttgt gcgtatgaaa atgcgtaaac cttgcccttc caagttacac cctttgatgc
     3121 aacatcaatg tagttagcct tgatgtcctt agatgcagat ggtgaaagtg ggttaatgta
     3181 gcctgcttct gccatttgac ctaattggtc gttaggtact tcaaatacat cagctgcctt
     3241 tgatgggtcc ttaccaacat ctgtcttagc gttagctgaa ccgtttggac tttgtacaac
     3301 acgaacctta atgttcttgt tcttcttagt aaagtcctta gcaatttgtt tgtagtaagg
     3361 tacttgttca gtatctaccc aaagtgtcaa gttagccttc ttatttgaag tgttacttga
     3421 acttgatgaa tttgatgagc catttgaaca agctgcaagt gacatagcag ctaaaacagc
     3481 agtactacct aaagccattt tcttccaaaa cttcatgact ttttcctcct aaaaaatcaa
     3541 aacaataaac aattcttaac aacagtagcc tttgctacct atggttgtaa gctttaataa
     3601 aattagttaa cgatagcttt tgtactatcc ttatcgaaga agtgagcctt attaacatcg
     3661 aagcccatcc tgaccatatc acctggttta tggaaatcac gtgaatttac gattgcagta
     3721 aattcagtgc catctacttc ttggtgaagt tgaatagttg cacctaaaag ttctgaaaca
     3781 acaacttttg attcaactac tgaatctggc caagtttcca taaatgcttc ttctgagtga
     3841 atatcttctg gacgaatacc gaagactaag tccttatcgt tgtatccttt ttcttcaagt
     3901 aattttgctt taccttcagg aataccaata ttcaaccctt tgccatcact aattcgaccg
     3961 ttcttatagt gaacattgaa gaagttcatt tgaggagaac caatgaatcc agcaacaaac
     4021 atatttactg gcttgttgta aacctcaagt ggtgacccga tttgctcaac tttaccaaca
     4081 gacataacaa caactctgtc agccaaagtc atcgcttcag tctgatcgtg ggtaacataa
     4141 atagttgtag ttcccaattc ttggtgaagc ttagcaattt ctgtacgcat caccacacga
     4201 agcttagcat caagatttga taatggttca tccattaaga agataggtgc ggcacgaacg
     4261 atcgcacgtc ctaatgcaac acgctgtctt tgaccaccgg ataaagcacc tggcttttta
     4321 tctagataat cttctagacc taacatctta gctgcttctt taaccttctt gtcgataaca
     4381 tccttaggtg tgtgacgaag cttaagacca aatgccatgt tgtcataaat ggtcatatgt
     4441 ggatacaaag catagttctg gaaaaccatc gcaatgtgac ggtcttttgg agcaacatcg
     4501 ttcattactt tatgatcgat ttccaaagtt ccttcactga tatcttccaa accggcaacc
     4561 attcttaatg tagttgactt accacaacct gatggaccaa cgaaaacgat aaattccttg
     4621 tctttaatgt gtaagttaaa gtcatttaca gaataccgat cgttacctgc atatttcttg
     4681 taaatatggt ttaaatcaac ctcaaccatt gtttctgaag tgggccgtaa aagttagaca
     4741 cttttacggc ttttactatg tccaaattaa acaaaaaaca aagacttaaa atttataatg
     4801 attggagaaa tggaaatgaa tccttatctg taatcgcttc tgaaataaaa ttaaatcgtt
     4861 ctaccctaca atatatggtt aatttaatag atcgtcatgg gatcgaaatc tatgataaat
     4921 ctaaggaatt gtttaccaaa gaatttaagg aaaaagccat tgttagatca ttaactggta
     4981 ataattcaat taatcagttg tctttagatc tgggactaag aagcaacggt atcttgtata
     5041 attggattcg cgaatataag aaaaatgggt ataatgtcgt tatcaagaag aaaggacggc
     5101 attccgttca tgagtcagaa gaagcaatcc caaacatcgc gcaaagattt agagaagcag
     5161 atccagcaac tcaaagaaga aaacttgaaa ttgcgtatcg tcaatgccta cgtaaaaaaa
     5221 ctaaacgcct tggatcagaa acatcacaag aaatagctca ggcagtcact gaactaaggc
     5281 aagaattcaa ggttagcttg gactatatat ttacagtctt ggctcaaaat aaagatctgc
     5341 ctttaatagc tcgcagtact tactacaaca ttatcaagcc taaagataag cctaagagaa
     5401 agaaaccagg tttcttatcc agaatgaaag aaatcttcga ttatcatcaa agacgttatg
     5461 gctatcgtcg tgtagctatg caattgcgaa aagaaggcta tgccgttaca gatcgagcag
     5521 ttcgatactg gatgcacaaa cttaagctca aaggtcgaag acgcaacaag cgtaagtatt
     5581 ccagttataa aggtacgatt ggtaagatag ctccaaatct gattcatcga ggcttctttg
     5641 ccatacgacc aaatatgaag tggtatacag acatcactga atttcactta aatggtcaga
     5701 aactttactt atcgccaatt cttgacggct gtggtggtga tatagtttct tattcaatct
     5761 cacgtcatcc ggatatgaat ttagttatgt ccatgctcaa taaggctttt gccaagcata
     5821 aggcacttaa tgcctgtatt tttcacacag atcagggctg tcaatatcag agcagagttt
     5881 atcagcgagc cttaaagctt catggcatca cgcaaagcat gtctcgtaaa ggtaattcca
     5941 tggatgatgg tctaatggaa aacttctttg gcctgctcaa aacagaaatg ttctatgatc
     6001 aggagtacaa gtatcacaat cttgaagaat taacacaagc aatcgaagaa tatatcaaat
     6061 actataacaa cgaaaggata aagagcagac taaaaggctt aaccccgatt gaatatcgag
     6121 atcaagcctt agtcagctag aacaaatatt tgtctaattt ttacgtctca gtacattccc
     6181 ctactctctt ttaattttta ttctttctct tcaaaaactt tttcaatttc actaagctta
     6241 agatcagcag tggttggaac gatgtaatca gcttctttca aaagcttttt attacctatt
     6301 ccaactgcaa attgatgagc agccttaatt gcttcaacac cagcttgggc atcttcaaag
     6361 ctaattactt catttgcctt caatccaaca agttcttgcg ctttgatata aatttctgga
     6421 tctggcttac catgatgaag agtggcagga tcaacaatcc catcaaattc atccataatt
     6481 cctagtctag ttaaaatctt aggtgcattc tttgaagcag aagcaattac catctttaag
     6541 ttttgcttct tagcatcact aagtaattct ggaatgccag gtaggatatc ctttggtgtc
     6601 attgtttcaa cttgctcaac aaacttagca ttcttttctg cagcaaattt ttctttttct
     6661 gcttcagtat acttatcttc ttgaccgcca tacttcaaga tcaaattaag cgaatccatt
     6721 cttgaaatac cacgtaaact atcaagttgt tcgtttgtta aagtaatgcc taattcctta
     6781 gccaaattat tccaagctgt aaggtggaaa cgtgctgagt cggttaagac accatctaaa
     6841 tcaaaaatta agcctttaag catttagttt taccttcctt caaatccaat ttattgtcat
     6901 gtaccatgac ttcaagatca tcaccagaaa gtaaagtaat cttgcattca tcatgatcta
     6961 cataaacttc aatcaaacga ccacgataat taatcttaaa gctgtagtga tcccaaccat
     7021 caggaacaaa tgggttgaac ttcaattgat catgatcgta acgcatccct gcgaaacctt
     7081 gaacaatcgc aagccatgaa ccactcattg aagtaatgtg taaaccatct actgtgtcgt
     7141 tattatagtt atcaaggtca agacgtgcag ttctttggta aagttcaact gccttttctt
     7201 tcttgccaag ttctgcagcc ataattgagt aaatacatgg tgaaagtgaa ctttcgtgaa
     7261 ctgttaatgg ttcatagaaa tcgaagttct tttccttttg atccatagtg tattgatcat
     7321 caaagaagta aataccttgt aaaacatcgg cttgcttaat aaatggtgaa cgtaaaatct
     7381 tgtcccatga ccagtgttga ttaattggac gttgatcttc aatctcagta acaggacgaa
     7441 tatctttatc taagaaatca tcttgttgca agaaaatgcc aagatcttca tcttctggta
     7501 agtacatgtt atccacaata tcttgccatt tagctttttc ttcgtcagta acacgaactc
     7561 tttcttgagc ttccttagta gcaagtggca aacgttccaa agtatatttg agaagccatc
     7621 tagccattgt gttagtaaac cagttgttgt ttacgttatt ttcatattca ttaggacctg
     7681 ttacaccgtg cattacgtat ttgttacgca tctttgacca gtgaactcta gctgcccaga
     7741 atcttgctgt accgactaaa acgtccatac cttcattctt aacgtaactg tcatcgccag
     7801 tgtaatcagt gtacatggct attgcgtggg gaatatctgc atttctgtga atttcttcaa
     7861 atgtgatttc ccattcgttg tgacattcaa taccattgaa ggtaaccatt gggaataatg
     7921 cacctggaag gccttgttct ttagcattat ggtaagcacc tggcaattga tcgtgacgat
     7981 attgaagaag tgctcttgta acacttggtt tagttactgc taaatacatt ggaacgataa
     8041 aggcttcggt atcccaataa gtggcaccac cgtatttttc accagtaaag cctttaggac
     8101 ctacattcaa gcgcttgtct tcaccgtaat atgtcataaa taattgacaa atattgaagc
     8161 gaataccttg ttgagctgca tcgtcgccgc tgatttctac atcacttgtt tcccaacgct
     8221 tcttccaagc ttctgtatga tctgctaagt tatcttcaaa actcttggtt tgaagtttac
     8281 tcatcaagtt gttagcaacg gctgcttgat ctttttcttc aacatcacga ctagttacaa
     8341 caatgacatc tttttcaagt tcatagcttt gaccttcgtc taattctact gagaatcttt
     8401 cactcaattt tgcatcttca gtagtaactt ctccatttac tgctacgcca ttattacgta
     8461 atgcttcttt caacaatact gtaaattgtg gtacgtcata tggatttttc ttagtcttta
     8521 cttgaatagt tttttcatct ttatcttcac caagtggaat ccagaagcga tcgccataat
     8581 tactgtcttc attaacaaca gtgccgtcta aagttgagtc aaaatcaatc tttgcatggc
     8641 cttcaagtac agttgcttta actttaatta gagcagcttc tttttgaaca atgtgaagaa
     8701 agcgttcaaa ttcaagctta actttgacat ctttaccttc atagataaaa cttctcgaaa
     8761 gaagaccttg atgcatgtct agtgataatt caaagtcgct aaatttaact ttagctaaat
     8821 cgaccttttc accatttaca ttgattccaa ttccaataaa acttggagca tttggtgttt
     8881 taccaaaata ttttggatat ccattcttcc accaaccaac aaccgtttta tctgggaacc
     8941 aaactcctgc taagtatgta ccttggaggc tatcacctga ataaccttct tcaaagtttc
     9001 ctctcattcc catgtaatcg ttaccgattg cagtcatact ttcctgcaat ctcttatctt
     9061 ttggatcaaa tttatgagta atgactttcc aaggatcaat ttcaaaaatt cgtttcatat
     9121 aatatctccc gcaatatttg aagtgcttat ttgtttacgc tttcattatc atgtaattga
     9181 aagcggttta caacatcaaa agccatgtta ccggtatcag aattaattaa taaaaaaaga
     9241 aggtagaact taacctacct tctttttctt gcttattaaa agtaactttt aatcaaccat
     9301 gattacaaag ccttttggag cgagtattcc tttttcaaaa tcttgactta attcagcttt
     9361 accatcaagt tttacatcat ttttagttgt attaaagtaa gcatgaatac tttctttgcc
     9421 ctctctaata acttcaatta gcctattttc tgttacgctt aacttgattt taccttcact
     9481 gagcgtttta tcatgagcta aacggaattt aattagctta tgaacccttt gccatacagg
     9541 tccatcttct tttgaccaat ccattggctt acgacaatct ggatcattat ctccagccat
     9601 ccccatttca gttccgtaat aaatacatgg actacctttt tgcatgaatt caaaagttaa
     9661 tgcttgaagc gccaagtctt gatcaccttt tgcaactgtc aaaattctgg cagtatcatg
     9721 tgaatcgagc atattcatca ttgcttcatt agtagaatca cgatatttca taagttgatc
     9781 agtcaagcgt gtagtcattt gatcagcagt taacttatgc ttgaaaaagt ggtcttcaat
     9841 ttgcaaagta tacggatagt tcattacacc tgtaaattca tcgccattaa gccatggacg
     9901 agccgaatgc caaacttcac ctacaatgta aaagtctggc ttaatatcag tgactgcttt
     9961 atggaacttc ttccagaagt gatgatcaac ttcatttgcc acatcaagac gccatgcatc
    10021 gatatcaaaa tacttcaccc agtatgtagc aatttctaac aagaaatctt gaacctcagg
    10081 attagcagta ttcaactttg gcatatgttc ttcaaaggca aaagtatcat attgcggatt
    10141 cttttcgcct tttaatggat tacgatatgg ttcaactgga tactcattaa tatggaacca
    10201 atctgcaaaa cgtgacttag atccattttg cactacatct tgccattgca ttgattgatc
    10261 acctaagtga ttaaatacgg catcgagcat aatcttcata ccacgtgcat gtgcttcgtt
    10321 aaccacttta gcaaataaat ctttatcacc aaattccgga tctacttgta aataatcaat
    10381 cgtgtcatat ttatgattag agctcgcttt aaaaattgga caaaagtata aaccattaac
    10441 gcctagcttt tgtaaataat ctaaatgatc taagacacct tgcaaatcac cgccataata
    10501 atcctctcta ccaggatgat cagttggatt ccatggctta atattcttag gatcgtttga
    10561 tttatcccca ttagcataac gttcagggaa tatctgatac caaacggtat tcttaaccca
    10621 gtctggtgtt ttgaccatat caatttcatg acaatatgga attttaaaat atgatccatc
    10681 ttcattgatt gcgtctttaa tatcatcgct aatcgcacgg tcaccataaa ctacacttgt
    10741 accgtcctta ccaataactt caaaagtgta ctttaaacga tgatatggtg catcaagtgt
    10801 aataccccaa tagtcttcaa cttgaccctc accgatcttt tccatttcag tagtttctgc
    10861 aaattttaat ggtaaataat tatcacagta atgaacaatt actttttcaa catcattttt
    10921 tgcagtgtga aaacgaacac gaacatgttt tggatcaact acaaaactat cttcactttc
    10981 tgttctgtgt cttaaagcgg ctaattgcat acattaattc tcctttttca aaacaacacc
    11041 cgcgtaaggc attaaagtta attttccatt agttaattga tattccccag cctttaatag
    11101 ttctttaata tactcttccg gaagatcgat tgaaatcttt ttattactta acgaaacagc
    11161 tacaatcgca ctctcattgc ccaaatcacg ttgataaacg taactgtcct taccagttga
    11221 gatcatatag taagttccgt cttggaatag tttttctttt ttcagattca gcatattttt
    11281 ataaaaagta aacatgctgt tatcagagtt gacttcattt gcaactgtta catcatcttg
    11341 actaattcca ttgagccaag gtttagcact agtaaaacca ttattttctg tatcgttcca
    11401 tggcattgga ccacgagccg gtaatttatg ggtatcgcta accatagcaa atgcggcatc
    11461 tctacttatt ccagcttttt gtgcctcttt tatccatgga gcaacagttt gatcttcaaa
    11521 ttgatcaaca ctagtaaagt gtaaattctt aagccctaat tcttcaccat aataaataat
    11581 cggaataccg cgttgcagat acatcaacat tgctaagctc tttgcttgag ttgaagtctt
    11641 cgcaattcta gtagcaagtc ttgccatatc gtgattattc caataaagcg tgggttgtga
    11701 tatatcagct aacgtttgtt gccaaaccac ctgattttgt ttaaaagctg ttaaatcaag
    11761 ctccttaggc tgatattgtg cggaataact cttatcgatt ttggaatcat cttctgtaaa
    11821 ataacgaaat gtaatcacgc aatccattaa atgattacgt ttattcgtat aatcaactgc
    11881 taagttaacg ctagcactag ctgcttcacc aagtaaaagt gcatctggat aatcttcttt
    11941 aatttgttca caaaatggtc tcatccattc ttgcacttgt ggcaaattag caaaaaacgg
    12001 ctctgcaata accggtttat catccatagc tggataattt tgtcttaagt ctgccttacc
    12061 aatatgaata aatgcatcaa gtcttaagcc gtctattccc ttctttaacc aaaattcagc
    12121 tatttcaagc atagcatgtc gaacttcagg gtttttccag ttaagatctg gcatccgttt
    12181 atcaaataag tgaaaatatg actgaccagt acctgccgga tcaggttccc agacgcttcc
    12241 tccaaagaaa gatccccaat tattaggttg tttattatca tgaccagcaa aaatgtagta
    12301 atcacgatat aaactatccg gattcttaat agcatcttgg aaccatggat gttgatctga
    12361 agtatgattt aagacaaaat ccataatgat atgaattcca gctttatgca gatctttaat
    12421 taaattctcc atatcttcca tcgttcccat gtgagaatca atcgcaaaat aatttgagac
    12481 atcataaccg ttatccactt gtggcgaaac aaaaacagga ttaagccaaa ctgcattaac
    12541 tcctaaattt tgtaaatagg gaattctttt acgaatacca ttaagatcgc caataccatc
    12601 gtcattgcta tcttgaaaag acttaggata aatttgataa ataattgcat gatcgtacca
    12661 atgtgacata attttttcct ccacgcatag tttagccgaa taaagaaacc gttttcaaca
    12721 tattgttaga tgttagcggt atcaaaaaac atccctttcg ggatgcactt ttaattgatg
    12781 ttaacgcgtc aaacgaacta cttcacgagc aatcattaat tcttcgttag ttggcacaac
    12841 caaggccttt accttggagt taggcattga aataaacttt tcgccatcgg ttctgttagc
    12901 cttgtagtca ggctttaatc ctaaatacct caacgatgac atcactccag cacgaattcc
    12961 agaatcatgt tctccaactc cggctgtgaa gataatccca tctacaccat ccatttcagc
    13021 tgcataagca ccaacatacc ggacaacacg attaatataa atacgccgag caagttcagc
    13081 acgtttttgt ttactttcaa taagatcacg catatctgac gacacaccag aaataccgag
    13141 caaccctgac tcatgattta gcatatcgat tccttcatca atcgacatac cagttttatc
    13201 cattatgtat cttaaagctg atggatcaaa gtcgccgctg cgtgtaccca ttgtcactcc
    13261 agtaagcgga ctaaagccca ttgaagtatc atatgattta ccatttttta cagcagttac
    13321 tgacgagcct gatcccaaat ggcaaactat taacttgcta atatttaaat ttttacccat
    13381 cattttagct gcacgaggtg caacatattc gactgaaatg ccatgagcac cgtacttacg
    13441 aactgcataa tcttcatagt acctatatgg aatagaatac aaataattaa tctcactaag
    13501 actttgatga tacgatgtat caaatactgc gatttgcgga atatctggaa ttatctgcat
    13561 aaaggcttct attccaactg tttcgggaat attgtgcaaa ggtgctaaat tacctaattt
    13621 aataagctgc tttaaattcg cttgattaat gacgactgaa tgtttgaaaa cctcaccacc
    13681 attaactact cggtgaccga caccagacaa ttctttcaga tctttaacca cgttaaattt
    13741 ctttaagctt tcaattaaca aactaactgc agcttcttga ttaggaatag gcgtattttt
    13801 gatgtattct ttactattaa gctttatttt aaataccgca ttagccatac caacacgatc
    13861 tgccataccc gatgcaacta ctttttcatt aggaaaagta aacaacttat atttaaagga
    13921 tgaactacct gaattaattg ctaaaacttt tttcataagt atgacctcta tttcaaaatt
    13981 cgatatctgc cataaattcc cttttgtttt aaagtatagc gctttcattg ctgacatgga
    14041 acgtttttat tcaataaaaa aatgcacagc aaccttgtag ttactgtaca tcttttttat
    14101 cttgttgttt ctcgtctaat taattgtggt ttaaccatta attcaccttg aggtgagccg
    14161 gattggtcaa ttttttgtaa taacatctta gcaagcatct tacccatttc aaaaatcggc
    14221 tgctttatag ttgtcaattt aggattagac acctgatcca gaaatacgcc gtcaaaacca
    14281 attacgccaa agtcttctgg aattcttgct ctttcatctt gcaattgtct aaccactccg
    14341 acggcaatac ggtcacttgc acaaataaaa caagtatctt ttttaaattc tttaaaatgc
    14401 tcatgtatta ccttttgtgc tgcatgacta tgattagata ctcgataaat ctgagggatt
    14461 aatcgatgct gttgtaaagt attaatatat cccgcttcac gtgaatattc aaatggttct
    14521 ttaatatcaa tcccaataaa aataatatgt ttgtataatc tgtccaatgc atattgggtc
    14581 gccatttttt cagctgtttg attatccgta tcaacaaaat cataaccgcg gtgattttct
    14641 ccaaataaga caaacggctt gtctagttta tcaaacatat cataatcttc attacgagcg
    14701 cctgtaatca agtacccatc tgctcgtcct ttttcaattc tagtgggatc aactactaat
    14761 tgcagcgcat attgcttggc atttaatcca cgagcaaaac caaaaagcag attagtataa
    14821 taaggttctg ttgtatcaat atcttctaaa gtaacgaact ttactacatt agatcgattg
    14881 ttaactaaag cttgtgcaat agaattaggg tgatagttca acttctccat agctttttca
    14941 accattgcac gtaattctgg cgttacttgt tctggatgat taatcacccg tgatacggtc
    15001 attttcgata catttgcttc tttagctaca tcagataaag ttgtcatatt ttcacct
//
