LOCUS       EU024644               11959 bp    DNA     linear   BCT 06-FEB-2008
DEFINITION  Paenibacillus sp. JDR-2 aldouronate-utilization gene cluster,
            complete sequence; and NADH-dependent flavin oxidoreductase and
            hypothetical protein genes, complete cds.
ACCESSION   EU024644
VERSION     EU024644.1
KEYWORDS    .
SOURCE      Paenibacillus sp. JDR-2
  ORGANISM  Paenibacillus sp. JDR-2
            Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Chow,V., Nong,G. and Preston,J.F.
  TITLE     Structure, function, and regulation of the aldouronate utilization
            gene cluster from Paenibacillus sp. strain JDR-2
  JOURNAL   J. Bacteriol. 189 (24), 8863-8870 (2007)
   PUBMED   17921311
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Chow,V., Nong,G. and Preston,J.F. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2007) Microbiology & Cell Science, University of
            Florida, Bldg. 981, Gainesville, FL 32611-0700, USA
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Paenibacillus sp. JDR-2"
                     /mol_type="genomic DNA"
                     /strain="JDR-2"
                     /db_xref="taxon:324057"
     misc_feature    <1..>11959
                     /note="aldouronate-utilization gene cluster"
     gene            1..1583
                     /gene="yesN"
     regulatory      1..6
                     /regulatory_class="ribosome_binding_site"
                     /gene="yesN"
     CDS             15..1583
                     /gene="yesN"
                     /note="two-component response regulator; signal
                     transduction protein; contains CheY-like receiver domain
                     and AraC-type DNA-binding domain"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0036286,DBD-SUPERFAMILY|0035607"
                     /protein_id="ABV90480.1"
                     /translation="MYKVLLVDDEIFVRKGLRNLIDWESLGYEICDEAGNGQEALEKIQ
                     LIKPDLVIADIRMPVLDGLELIRKVTEEGVHSPTFIIVSGYHDFQYAQRALRYGVHDYI
                     LKPIDEAELETTLKALSGTLGLKKLATIAGDSLVTDSIVETLVQGQFSEADNGALCAAL
                     QMPEFSSFDYVLVELHASAQSAGTEWQAKELAAVVQSVSATPSSKIPVYEQTSGLFGLL
                     IDRKPFLPPEIRLESAYNSLHAAIAKSSGRPVTLYIGKTVERLQEMCLSYRAANEAMSY
                     KYAEQGSSVIYANQVQGTPLYYFDVDSELYSRLLERLEENNADLYEQDIELLFRQFVEK
                     RFAPNAVANTITRFVIGIINIIRKMEGDEKGLSKLPFIMDWQGSNSRLQDLKGLFTDFL
                     REAAVYLAELRNEQSKGGIERIKKYIEANYTENISLKSIAGKFYMNSVYLGQLFRKTYG
                     IYFNDFLLQIRIGEAKKLLRQTDLRMYEIAEKVGFQNADYFVTQFEKLEKVTPTDYRNK
                     LLGKK"
     gene            1590..3317
                     /gene="yesM"
     regulatory      1590..1594
                     /regulatory_class="ribosome_binding_site"
                     /gene="yesM"
     CDS             1599..3317
                     /gene="yesM"
                     /note="two-component histidine kinase sensor; signal
                     transduction protein"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="ABV90481.1"
                     /translation="MGRFHWNHLKLRDKLLLMYVFCVFIPIVLTNIVFYNVTTNNIKNQ
                     KSHDADIALEKLQGELRAVIDEAAGISYLYYIDPMLNQLLDKKYDSQIEYVEAFNNIRS
                     VFNKSEQAYKTTSATVIYTDNPTVLSSGPIMPLSETEKEADWYRAFMKTNVSYPLFIQD
                     GDTFSLVQRLNYVKGNGYNNLIKIDLNMATVKQLFALSGFEGSIYFLNPEGAVLYSNDS
                     SVDRHSQTLPMPKNSLSFDKVYTNNNYLNDWSLHGVINEGSFLHDVRKSGSFVIFLALI
                     NFVLPSLIIAALSRSINKRLVKIVKHMKKVKNQHFETIPLDDARDEIGQLTGEFNRMTE
                     RIDNLITDVYQADIQKKDLEIRQRQAQLHALHSQINPHFLFNSLETIRMRSLMKGETET
                     AKTIHYMAKIFRKSISWKRSWVSIREEIELTECFLEIQKYRFGDKLQYQITVEDTVYDQ
                     MIPKMTFLPFVENASIHGIESSPGIGLIQIHIGIAGNKLMFRLSDNGIGMSQAKLSELL
                     NYLRLDDSIGDNVGMKNVYTRLKLCYKDSFEFDIASEEGHGTTVELRLPLDMNGQ"
     regulatory      3363..3368
                     /regulatory_class="minus_35_signal"
     regulatory      3391..3399
                     /regulatory_class="minus_10_signal"
     misc_feature    3399..3412
                     /note="catabolite repression element"
     gene            3432..5163
                     /gene="lplA"
     regulatory      3432..3439
                     /regulatory_class="ribosome_binding_site"
                     /gene="lplA"
     CDS             3451..5163
                     /gene="lplA"
                     /note="substrate binding protein; ABC-type sugar transport
                     system component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR6|3.A.1.1.29"
                     /protein_id="ABV90482.2"
                     /translation="MVNKKLLTVSLAAAMLAVTASACGGNNDNSNANSGKSGNSGNAAS
                     NSASPEADDKTPVTFSYYSFTSQKDALASDTVIGKELQQQTGVDWKMEFLVGDPQTKSG
                     VMIASGDYPDVIVPEGEIDKLLDAGAFIPLDDLIEKYGPNIKRVYGPYFDKFRQADGKM
                     YFLPFGANQGYIGDPGISQGAFWIQRSVLKEAGYPKIKTLDQYFDLIKQYQEKHPQVDG
                     KDTIGFASLAGPADSFFSITNPSMHLAGYPNDGDVIVDMNTHEAKTYAATDIEKKWLQK
                     LNEVNAEGLFDPETFTANKDQFLAKLTSGRVLGYFNYAWQVGDATNNLKKAGIDEKRYA
                     PLPIVFDENTKDQYVDPPSFVNNRGIGISVKAKDAVRIIKYFDNLLKEENQVLVQWGVK
                     DQNYTVDANGRYVMDAQQIADRNDPEKKRTVGWQYFEYSWPRYGNNSVLADGNSYGVGN
                     QPEVAYAGYTDGDKALLDAYGVKTFSEYFSKPDDRSWYPAWSINKGQGTPEQIFQQKAG
                     DLQKKFVPKLVLAKPSEFDSIWSDYTGQMGKLDVKGYETFVTKVVQDRIAGKW"
     misc_feature    3600..3613
                     /gene="lplA"
                     /note="catabolite repression element"
     gene            5237..6224
                     /gene="lplB"
     regulatory      5237..5244
                     /regulatory_class="ribosome_binding_site"
                     /gene="lplB"
     CDS             5253..6224
                     /gene="lplB"
                     /note="lipoprotein permease; ABC-type sugar transport
                     system component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR7|3.A.1.1.29"
                     /protein_id="ABV90483.1"
                     /translation="MENIHEVTPSVVRSPKASESRLQLFFKKLLQQKVLVFMSMPFVLW
                     LFLFKYVPLWGWTISFQKFRPAKDLFDQEWVGWKNFNFLFNDDSFYRVLRNTIVMSSIN
                     LVLGFVTAIVLAILLNELRQIMFKRVVQTISYLPHFISWVVAANIISSALAPEGIVNIL
                     LTRMHLIDQPILWLGKGNYFWGILGASEVWKNVGWNTIIYLAAITTIDPSQYEAAEIDG
                     ANRLQRILHITLPGLKSVIVILLIMNLGNILESGFEPQYLLGNGMTVDYSENLDIFVLK
                     YGMNMGNYSLATAAGMFKTVVSFIFLLSANSIAKRLGESRLF"
     gene            6249..7184
                     /gene="ytcP"
     regulatory      6249..6255
                     /regulatory_class="ribosome_binding_site"
                     /gene="ytcP"
     CDS             6264..7184
                     /gene="ytcP"
                     /note="permease; ABC-type sugar transport system component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR8|3.A.1.1.29"
                     /protein_id="ABV90484.1"
                     /translation="MKESSAKARYSSLPDKIFDTSNIVFMLLVVTVTLYPFLNMFALSF
                     NDANDSIRGGIYAWPRMWTWDNYSYIFNEASIYHATLISALRTIAGTITSVFCTAMLAY
                     TISRQEFVLRKFVTLVAIFTMYFSGGLIPGYLLIKELHMINSFWVYIIPGIIGVFNMIV
                     IRSFIEGLPDGIMESAKIDGAGEFITFMRIVLPLTVPALATVSLFVAVSQWNSWFDVFL
                     YNSSHLNLSTLQYELMKILQTSNTAASSTNAGDQFAAGQSGVTAVTPTSIRATMTIVAS
                     LPIILVYPFLQKYFVKGMTVGGVKG"
     regulatory      7211..7216
                     /regulatory_class="minus_35_signal"
     regulatory      7231..7239
                     /regulatory_class="minus_10_signal"
     gene            7268..9344
                     /gene="aguA"
     regulatory      7268..7274
                     /regulatory_class="ribosome_binding_site"
                     /gene="aguA"
     CDS             7281..9344
                     /gene="aguA"
                     /note="alpha-glucuronidase A; glycosyl hydrolase family 67"
                     /codon_start=1
                     /transl_table=11
                     /product="GH67"
                     /protein_id="ABV90485.1"
                     /translation="MGDNGYAAWLRYDQVKDETRLEQYAGWTGELVLPAGVPMEGIMKT
                     AAVELSRGIRSMLGTTPSVTHEASGQRFIVLEVLGGGSWIDQAAGDAAALSDEGYFLKT
                     VREAEKEYIIAAGKSEKAVLYAVFHLLRLMQSGTAIDQLNLVESPKYSLRMINHWDNMD
                     GSVERGYSGRSIFYDNNKVLSDSERIRDYARLMASVGINGIAINNVNVHREETFLITEK
                     LLPDVVRIAEVFGEYGIKLFLSVNYAGTIEIGGLETADPLDPAVRGWWKEKAAEVYRYI
                     PDFGGFLVKADSENRPGPFTYGRDHADGANMLAEALEPFGGLVLWRCFVYNCHQDWRDR
                     STDRARAAYDHFKPLDGRFKDNVILQIKNGPMDFQVREPVSPLFGAMEHTNQMMEFQIA
                     QEYTGQQKDVCFLIPQWKEVLNFDTYVKGAGSTVKEIAAGSVHAYTHSGITAVSNIGND
                     ENWTGHHLAQANLYGYGRLIWNPELSSEEIAAEWAAQTFGGNKNVQDVLVNFLLESLSI
                     YENYTAPLGVGWMVTPHYHYGPDIDGYEYSKWGTYHFADHKGIGVDRTAQTGTGYSSQY
                     ALPNAEVYDSLEACPDELLLFFHHVPYTHQLKSGKTVIQHIYDTHFAGVERVEYWMNRW
                     QELEGLVDGDRFRHVTGRMNWQRENAKQWRDIVNTYFFRKSGIPDTHNRVIY"
     misc_feature    9175..9188
                     /gene="aguA"
                     /note="catabolite repression element"
     gene            9352..10397
                     /gene="xynA2"
     regulatory      9352..9359
                     /regulatory_class="ribosome_binding_site"
                     /gene="xynA2"
     CDS             9372..10397
                     /gene="xynA2"
                     /note="endoxylanase; glycosyl hydrolase family 10"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM22|GH10"
                     /protein_id="ABV90486.1"
                     /translation="MSYTSELPALRSVYKDYFDIGAAVNLTTIASQKDVLTAHYNSLTA
                     ENDMKFERVHPQEGQYTFEAADKIADFAAANAMKLRGHTLVWHNQTPDWIFQNANGSPV
                     DRETLLARMKSHIEKVVGRYKGIIYGWDVVNEVIDDKNGVWLRESKWLNLAGEDFIAKA
                     FEYAHAADPKALLFYNDYNECIPEKRDKIIRIVQSLQAKQVPIHGIGLQGHWNLNGPSL
                     AEIREAIERYAATGLKLQVTELDISVFDHDDKRTDLTEPTADMLERQAERYGQVFELFR
                     EYKEAITAVTFWGAADDYTWLDNFPVRGRKNWPFVFDANHEPKASFRKITDWQEAGQID
                     S"
     gene            10376..11959
                     /gene="xynB"
     regulatory      10376..10382
                     /regulatory_class="ribosome_binding_site"
                     /gene="xynA2"
     CDS             10394..11959
                     /gene="xynB"
                     /note="beta-xylosidase; glycosyl hydrolase family 43"
                     /codon_start=1
                     /transl_table=11
                     /product="GH43_12|GH43"
                     /protein_id="ABV90487.1"
                     /translation="MTYASTGKITYRNPVLPGFYPDPSAVRVGEDYYMVTSTFEYFPGV
                     PVFRSKDLVHWEQIGHVLTRESQVNLLSRNSSEGIYAPALRYHEGVFYMITTDVYGIGN
                     FYVTATDPAGPWSDPIRIPYGNIDPSLFFDDDGKAYVSAQAGFGETSHIIQYEIDILTG
                     EALSEPVVVFEGDEGPWVEGPHLYKINGFYYMMTASGGTGPMHREIIGRGTSPYGPFEM
                     LPHPILTHNELQDHPIQYTGHVELLDDVRGQWWALFLGVRPQGGNGSVLGRETFLAPVR
                     WSEDGWPMIDNNEGHVSMVMEGPAIRESKTSMAGMDKSVTTKFTSEGLSLEWMYNRVIP
                     GPEELSLTEKEGCLRLSGNAKGLRDGGANVFVCRRQQHHRMSIETRLTFVPGREGEQAG
                     IAARLSDKSHYSIGITKKDGQTGILVTAMAQGSGTEVFTPIEEQVPVRLSIRSDEKEYE
                     LLYTLGGASAEWVSLRTLPVEALTPDADGAFTGVCLGMFAAGTEEGHSAPAYYDYFEYR
                     PESG"
ORIGIN
        1 agggggcctt gcatatgtac aaagtgttat tggtggatga tgagattttt gtgcgcaaag
       61 ggttaaggaa tttaatagac tgggaatcgc ttggctatga aatttgcgat gaagcgggta
      121 acggacagga agcattagag aaaatacagc tgattaagcc tgatctcgtt atcgcggata
      181 tccggatgcc agtgctggac ggattggagc ttatccgcaa ggttaccgag gagggcgtac
      241 atagcccgac ctttattatc gtcagcgggt atcatgattt ccagtacgcg cagcgagctc
      301 tccgttacgg agtccatgac tatatattaa agcctatcga tgaagcggaa ctggagacaa
      361 cgttgaaggc gctttccgga acgttgggat taaaaaagct ggccacgatc gcgggggaca
      421 gcctggtcac ggactctatt gtggaaacgc ttgttcaagg ccagttctcg gaggccgata
      481 acggagcttt atgcgcggcg ctgcaaatgc ctgagttctc cagcttcgat tacgtgctgg
      541 ttgagcttca tgccagcgcg caatccgcag gcaccgaatg gcaggccaag gagctggcag
      601 cagtcgtgca gtcagtgagc gctactccaa gcagcaaaat tcctgtctat gagcagacga
      661 gcggtttatt tggcctgctt attgatcgga agccgttttt accgccggaa atacgacttg
      721 aatctgccta taacagcttg catgcggcca ttgcgaaatc ttcgggcaga ccggtcacct
      781 tatatatcgg caagaccgtg gaacgactgc aagaaatgtg cctatcctac cgggcggcga
      841 atgaagccat gtcctataaa tacgccgagc aaggcagctc cgttatctat gcgaatcagg
      901 tacaggggac ccctctgtat tatttcgatg tggattcgga gctgtacagc cgactactcg
      961 agagactgga agagaataac gcggatttgt atgagcagga tatcgagctt ttattccgcc
     1021 aatttgtcga gaagcggttt gctcccaatg cggttgccaa tacgattacc cggtttgtga
     1081 tcgggattat taatataatt cggaagatgg agggagacga gaagggactt agcaagctcc
     1141 cgttcattat ggactggcag ggcagcaata gccgtctgca ggacttgaag ggactattca
     1201 cggatttcct ccgcgaggct gccgtctatt tggcagagct tcggaacgag cagtccaagg
     1261 gcgggattga acgcatcaag aaatacatcg aagcgaacta tacggagaat attagcctta
     1321 agagcattgc aggaaaattt tatatgaact ccgtttattt gggtcagctg ttccgtaaaa
     1381 cctatgggat ctatttcaat gattttctac tgcagatccg gatcggagaa gccaaaaagc
     1441 tgcttcgcca gaccgatctt aggatgtacg agattgcgga gaaagtgggc tttcagaatg
     1501 ccgattattt cgtgacccaa tttgagaagc tggagaaagt gacaccgacc gattaccgca
     1561 acaagctgct gggcaaaaaa taaacgggtg gagatttgat gggcagattt cattggaatc
     1621 atcttaaatt gcgggacaag cttttgctga tgtacgtctt ttgcgtattt atcccgatcg
     1681 tattgacgaa tatcgtcttc tataacgtta caaccaacaa tattaaaaac caaaaatcgc
     1741 atgacgccga catcgccctg gagaagcttc agggcgagct tcgtgctgtc atcgatgaag
     1801 ccgcgggtat ttcctacctg tattatatcg atccgatgct gaatcagctg ctggataaaa
     1861 agtacgattc ccaaatcgaa tacgttgaag cttttaataa tatccgaagc gtcttcaaca
     1921 aatccgagca ggcttacaaa acgaccagcg ctacggttat ttataccgat aatccaactg
     1981 tgttatcctc cggacctatt atgccgctta gcgaaacgga gaaggaagcc gactggtacc
     2041 gggcttttat gaagacgaat gtttcttatc cgttgtttat acaggatgga gacacgttta
     2101 gccttgttca aagactgaac tatgtcaaag gcaacggcta taacaatctg atcaagattg
     2161 atctgaatat ggcaacggtc aagcagttgt ttgcgttatc cggctttgag ggcagcattt
     2221 acttcttgaa tccggaaggt gccgtcctct attccaacga ctccagcgta gacaggcatt
     2281 cacagacgct tccgatgccg aaaaactctc tctcctttga caaggtttac acgaacaaca
     2341 attatttgaa cgactggtcc ctgcatggcg tcattaacga agggagcttt ctgcatgacg
     2401 tgcggaaatc gggttccttt gtcatttttt tggcgcttat taactttgtg ctgccttctt
     2461 tgattatcgc ggctctctcc agatccataa acaaacgtct cgtcaagatt gtaaagcata
     2521 tgaaaaaggt gaaaaaccag catttcgaga cgatcccgct tgatgatgcg cgcgatgaga
     2581 tcggccaatt aacgggtgaa ttcaaccgga tgaccgagcg gatcgacaac ctcattaccg
     2641 atgtctatca ggctgatatt cagaagaagg acctggagat ccgccagcgc caggcacagc
     2701 ttcatgccct tcacagccag atcaatcccc acttcctgtt caactcgctc gagacgattc
     2761 ggatgcggag cctgatgaag ggggagaccg agaccgcgaa gaccattcac tatatggcga
     2821 agattttccg gaagtcgatt tcctggaaac gcagctgggt gtcgatccgg gaagagattg
     2881 agctgacgga atgcttcctt gaaattcaga aatacaggtt tggcgacaag ctgcaatacc
     2941 agatcaccgt cgaagatacc gtatacgacc agatgattcc gaagatgacc ttcctgccgt
     3001 ttgtcgagaa cgccagcatt catggcattg aaagctcgcc gggaatcggt cttattcaga
     3061 ttcatatcgg aatcgccggc aacaagctga tgttcaggct gtccgataac gggatcggca
     3121 tgtcccaagc caagctgtcg gagctgctta attacctgcg tctggatgat tccatcggtg
     3181 acaatgtcgg catgaaaaac gtctataccc ggctgaagct atgctataaa gactcgtttg
     3241 aattcgacat tgctagcgaa gaaggtcatg gaacaaccgt tgagctgcgt cttcctctcg
     3301 acatgaacgg gcaataaatg acaaaaaagt tgaactatgc ttttcaaagt acgaactaaa
     3361 gtttgagggg taatcaagcc ttcggattcg ttttatgatg aaagtgctta caacaaacac
     3421 gttgaaatga cgaaaggggt ctaaacagaa atggttaaca aaaagctttt aactgtcagc
     3481 ttggctgctg ctatgttagc tgttacagca tccgcttgcg gcggcaataa cgataacagc
     3541 aatgcaaata gcggtaaatc cggcaatagc ggcaacgcag cttccaactc cgcttctcct
     3601 gaagcggatg acaaaacacc cgtcactttc tcgtactaca gcttcaccag ccaaaaggat
     3661 gctcttgcga gcgacaccgt tatcggtaaa gagctgcaac agcagaccgg cgtagactgg
     3721 aaaatggaat tcctcgttgg cgatccgcaa acgaaatccg gcgttatgat cgcaagcggc
     3781 gactatccgg acgtaatcgt tccggaaggc gaaatcgaca agctgcttga cgctggcgca
     3841 tttattcctc ttgatgatct gatcgagaaa tacggtccaa acatcaagcg cgtatacggt
     3901 ccttattttg ataaattcag acaagcagac ggcaaaatgt acttcctgcc gtttggcgcg
     3961 aaccaaggct atatcggcga tcccggcatc agccaaggcg cattctggat ccaacgctcc
     4021 gttctgaagg aagcgggata tccgaagatc aagacgcttg accagtactt cgatctgatc
     4081 aagcaatacc aagagaagca tccgcaagta gacggcaagg acacgattgg ctttgcatcc
     4141 cttgcaggtc ctgccgacag cttcttctcg atcaccaacc cttccatgca ccttgcgggc
     4201 tacccgaatg acggcgacgt tatcgtcgac atgaacacgc atgaggctaa aacttacgca
     4261 gctacggata tcgagaaaaa atggctgcaa aagctgaacg aagtaaacgc ggaaggcttg
     4321 ttcgatcctg agacctttac agcgaacaaa gaccaattcc tggctaagct gacctccggc
     4381 cgcgtacttg gctacttcaa ctacgcatgg caggttggcg acgcgacaaa caacctgaag
     4441 aaagccggca tcgacgagaa gcgttacgct ccgctgccaa tcgtattcga cgagaatacg
     4501 aaagaccaat acgtagatcc gcctagcttt gttaacaacc gcggtatcgg tatctcggta
     4561 aaagcgaaag acgctgtacg cattattaag tacttcgaca accttctcaa agaagagaac
     4621 caagttctcg tacagtgggg cgttaaagac cagaactaca ccgtggacgc taacggccgt
     4681 tacgtgatgg atgcgcagca aatcgctgac cgcaacgacc ctgagaagaa acgcacagtc
     4741 ggatggcagt actttgaata cagctggccg cgttacggca acaactccgt actggctgac
     4801 ggcaactcgt atggcgtagg caaccagcct gaagtggctt acgcgggtta cacggacggc
     4861 gacaaagcgc tgctggacgc ctacggcgtg aagaccttct ccgaatactt ctcgaagcct
     4921 gacgatcgct cctggtaccc ggcatggagt atcaacaaag gtcaaggcac gcctgagcag
     4981 atcttccagc aaaaagccgg cgacctgcaa aagaaattcg ttccgaagct tgtacttgcc
     5041 aagcctagcg aattcgactc gatctggagc gactatacag gccaaatggg caagcttgac
     5101 gtgaaaggct atgaaacctt cgttacgaaa gtagttcaag accgtatcgc gggcaagtgg
     5161 taagcaaata aaagtagacg gaaggccgga aagctgattc cggccttccg atctattgga
     5221 ttcatgcagg atgctagaga ggggaagagc aagtggagaa catacatgaa gttacaccat
     5281 ctgttgtcag gagtcccaaa gcaagcgaat cacggcttca actgtttttc aaaaagttat
     5341 tgcagcaaaa agtgctcgtg tttatgtcga tgccgtttgt attatggctt ttcctgttta
     5401 agtacgtacc gctctggggc tggaccatct cctttcaaaa gttcagaccg gcaaaggatt
     5461 tgttcgatca ggagtgggta ggctggaaaa acttcaactt cctgtttaac gacgactcct
     5521 tctatcgcgt gttaagaaat acgatcgtta tgagctccat caatcttgta ttaggttttg
     5581 ttaccgcaat cgtattggcg attttattaa acgagcttcg ccaaattatg ttcaagcggg
     5641 tcgtacagac gatcagctac ctaccgcatt ttatttcctg ggtcgttgcg gccaatatta
     5701 tttcttcggc gcttgcgccc gaagggatcg ttaatattct attgacgaga atgcatctga
     5761 ttgatcagcc tatcttatgg ctgggcaaag ggaattactt ctggggcatc cttggcgcat
     5821 cggaagtttg gaaaaacgtc ggctggaaca ccattatcta tttggcggct attacaacca
     5881 ttgacccttc ccaatacgaa gctgccgaga ttgacggggc gaaccgttta caacggatcc
     5941 tgcatattac tttaccgggt ctgaagtcgg ttatcgtaat cctgttgatc atgaaccttg
     6001 gaaatattct ggaatcggga tttgagccgc aatacttgct cggcaacggg atgaccgtgg
     6061 actattccga gaaccttgat atatttgtgt tgaaatacgg catgaacatg ggcaactatt
     6121 ccttggctac agcagccggc atgttcaaaa cggtcgttag ctttatcttc ctcttatcgg
     6181 ccaactcgat tgcgaagcgg cttggcgaga gcagactgtt ctagatttaa ccgaaagttg
     6241 gacgaagcaa ggaggctatc gacatgaaag agagttcggc caaagccagg tacagctctt
     6301 taccggataa gatttttgat acaagtaata tagtttttat gttgctcgtc gtgacggtta
     6361 cgctatatcc tttccttaat atgttcgcgt tgtcgttcaa cgatgcgaat gactcgatcc
     6421 gcggcggcat ttatgcttgg ccaaggatgt ggacatggga taactacagc tatattttta
     6481 atgaagcatc catctatcac gctacgttga tctccgcctt gcgtacgatt gccggcacga
     6541 ttacttccgt cttctgtacg gctatgctgg cttatacgat cagccgccag gagtttgttc
     6601 tgcgcaaatt cgttacgctg gtcgcaatct tcacgatgta cttcagcgga ggtctcattc
     6661 cgggatacct gctgatcaaa gagctccata tgattaactc gttctgggtt tatattatcc
     6721 ccggcattat cggcgtcttt aatatgatcg taatccgatc ctttatcgag ggattgccgg
     6781 acggcattat ggaatcggcg aaaattgacg gagcgggcga attcattacc ttcatgcgaa
     6841 ttgttttgcc gctgaccgtg cctgctcttg cgaccgtttc gctcttcgta gcggtgtctc
     6901 aatggaattc ctggtttgac gtatttctgt acaactcttc gcatcttaac ctgagcacct
     6961 tgcaatacga attaatgaaa atattgcaaa cctccaatac ggcggcatcg tcaaccaatg
     7021 caggcgatca gtttgcagcc ggacaaagcg gcgtaacggc ggttacgcca acctcgatcc
     7081 gtgcgacgat gactattgtt gcgagtctgc cgattattct ggtatatccg ttcctgcaga
     7141 aatactttgt taaagggatg accgtaggcg gcgttaaagg ttaatcgctt gaatcacgcg
     7201 tccctcgact ttgcatcggg cggacgcttt ttttctaatt ttggctgttt gtataacaat
     7261 tcttattagg gggattagag atgggagaca acggatacgc ggcatggctg agatacgatc
     7321 aagtgaagga tgaaacgcgc ttggagcaat acgcaggctg gacgggtgag ctcgtgctgc
     7381 ccgcgggagt accgatggaa gggatcatga agacagccgc cgtggaattg tcgcggggaa
     7441 tccgctccat gctgggcaca acgccttcgg ttacgcatga agcatcaggg cagcgtttta
     7501 tcgtgctgga ggttcttggc ggcggctctt ggatagatca agcggccggc gatgcggcgg
     7561 ctctatctga tgagggctac ttcttaaaaa ccgttcgaga agcggagaag gaatatatta
     7621 tcgcggccgg caagtccgag aaagcagtac tgtacgcggt attccatctg ctgcgcctga
     7681 tgcagtccgg gactgcgatt gatcagttaa atctggtcga gtcgccgaaa tacagccttc
     7741 gcatgattaa ccattgggat aacatggacg gcagcgtgga gcgcggttac tccggccgtt
     7801 cgatttttta cgataacaac aaagtgctgt ccgattcgga acggattcgc gattacgccc
     7861 gtctgatggc atcggtcgga attaatggca tcgcgattaa taacgtaaac gtccaccgcg
     7921 aagagacatt ccttattacg gagaagcttc tgccggatgt tgtccgtatc gccgaggtat
     7981 ttggcgaata cggcataaag ctgttcctga gcgtgaacta tgccggaacc attgagattg
     8041 gcggcctgga gacagccgat cctcttgatc cggccgtgcg cggctggtgg aaggagaagg
     8101 cggctgaagt gtaccgttat attccggact ttgggggctt ccttgtgaaa gccgattccg
     8161 agaatcgccc cggtccgttt acctacggac gcgaccatgc ggacggagct aacatgctgg
     8221 ctgaagcatt ggagccgttt ggcggacttg tgctctggcg gtgcttcgtt tacaactgcc
     8281 atcaggactg gcgggatcgc tcaaccgacc gtgcgcgtgc cgcttacgat cactttaagc
     8341 cgctggacgg acgcttcaag gacaatgtga ttttgcagat taagaacggt ccgatggact
     8401 tccaggtccg cgagccggtc tcgccgctct ttggcgcgat ggagcatacc aaccagatga
     8461 tggaattcca gattgcccag gaatatacgg gccagcagaa ggatgtctgc ttcctcattc
     8521 cgcagtggaa ggaagtcttg aactttgaca cttacgtcaa aggtgcgggc agcacggtga
     8581 aggagattgc ggcaggcagc gtccatgcct atactcatag cggcattacg gccgtcagca
     8641 atatcggcaa cgacgagaac tggaccgggc atcatctcgc gcaggcgaat ttgtacggct
     8701 acggacgctt aatctggaat ccggagcttt cttcggagga gattgcggcg gaatgggctg
     8761 cccaaacgtt tggcggcaat aagaacgtac aggatgttct tgtaaacttc ctgctggagt
     8821 cgttgtccat ttacgagaat tatacagcgc cgctaggcgt aggctggatg gttacgcctc
     8881 attatcacta cggtccggat attgacggct acgaatattc caagtgggga acataccatt
     8941 ttgccgatca taagggaatt ggggttgacc gtacggctca gacgggtacc ggctacagca
     9001 gccagtatgc tttgccgaac gccgaggtct acgacagcct ggaggcttgt ccggatgagc
     9061 tgctgctctt cttccatcat gtgccttaca cgcatcagtt gaaatccggc aagacggtta
     9121 ttcagcatat ttacgatacg catttcgccg gcgtggagcg ggttgaatat tggatgaacc
     9181 gctggcagga gcttgagggt ctcgtggacg gcgaccggtt ccgccatgta acgggacgca
     9241 tgaactggca gcgtgaaaat gccaagcaat ggcgcgacat cgttaatacg tatttcttcc
     9301 gcaaatccgg tattccggat acccataacc gggtgattta ctaatctaca aggaaagagt
     9361 tggtgcagga tatgtcatat acttcggagt tgccggcact tcgttccgtt tataaggatt
     9421 actttgatat tggggcagct gtgaatctga cgacaattgc atcccagaag gatgttctta
     9481 ctgcccatta caacagtctg actgccgaaa atgacatgaa gttcgaacgc gtgcatccgc
     9541 aggaagggca gtatacgttc gaagcggcgg acaagatcgc tgactttgcg gctgcaaacg
     9601 ccatgaagct gcgcgggcat acgctggtgt ggcacaatca gacccctgac tggatattcc
     9661 aaaatgcgaa tggatcgcca gtcgataggg aaacgctgct tgcccggatg aaaagccata
     9721 tcgaaaaggt tgttggccgg tataaaggca ttatttacgg ctgggatgtt gtgaacgagg
     9781 ttatcgacga taaaaacggt gtctggctta gggagtccaa atggctaaac ttagccgggg
     9841 aagattttat cgcaaaagcc ttcgagtatg cgcatgccgc cgacccgaaa gcgcttctgt
     9901 tctacaatga ttacaacgag tgcattccgg agaagcggga taaaattatc cgtatcgttc
     9961 aatcgctgca ggctaagcaa gtgccgattc acggtatcgg cctgcagggg cattggaatt
    10021 tgaacggtcc aagtcttgcc gagatccggg aagccatcga acgttacgcg gcaacgggtc
    10081 ttaagctgca ggtaacggag ctggatattt cggtattcga tcatgacgat aagcggacgg
    10141 atctgacgga gcctacggca gacatgctgg agcggcaggc ggaacgttac gggcaggtat
    10201 tcgagctgtt ccgcgaatac aaagaggcga ttaccgccgt tacgttctgg ggcgcagccg
    10261 acgattacac ctggctggat aacttcccgg tacgcgggcg gaaaaattgg ccgtttgtgt
    10321 ttgatgccaa ccacgagcct aaagcttcct tccggaaaat aaccgattgg caggaagcgg
    10381 ggcaaatcga ttcatgacat acgcatcaac aggcaaaatc acgtacagga acccggtact
    10441 gccgggcttt tatccggatc caagcgctgt tcgggtaggc gaagattatt acatggtgac
    10501 cagcaccttt gaatattttc cgggagttcc ggtgtttcgc agcaaggatc tcgttcattg
    10561 ggagcagatt ggtcatgtgt taaccaggga aagccaagtg aatctgctga gccgcaacag
    10621 ctcggagggc atctatgcgc ctgctcttcg ttatcatgaa ggcgtatttt atatgattac
    10681 aacggatgtg tacgggatag gcaacttcta tgtaaccgca acggatccgg caggaccatg
    10741 gtccgatccg attcggattc cttacggcaa tatcgatcct tctctgtttt ttgacgatga
    10801 cggcaaagcg tacgtctcgg cgcaggccgg ctttggcgag acgtctcata ttatccaata
    10861 cgagattgat atcctgacgg gcgaggcctt atcggagcct gttgttgtat ttgagggaga
    10921 cgaagggcct tgggtagaag ggccgcatct gtacaaaata aacggctttt attatatgat
    10981 gaccgcatcc ggcggaacgg ggccgatgca cagggagatt atcggccgcg gcacctctcc
    11041 ttacggaccg tttgaaatgc tgccgcatcc gattctgacg cataacgagt tacaggatca
    11101 tccgattcaa tacaccggcc atgttgagct gctcgatgac gttcgtggcc agtggtgggc
    11161 attgtttctt ggcgtccggc cgcaaggcgg caacggcagc gtgcttggcc gcgagacgtt
    11221 ccttgctccc gtacgctgga gtgaagacgg ctggccgatg attgataata acgaaggtca
    11281 tgtatcaatg gttatggagg gaccggctat ccgggagagc aaaacctcca tggccggcat
    11341 ggacaagtcg gtgaccacca agttcacttc cgaagggctt agtctcgaat ggatgtataa
    11401 ccgggtgatc cccggaccgg aggagctgtc tttaacggag aaggaaggct gccttagact
    11461 aagcggcaat gccaaaggtc ttcgcgacgg aggagctaat gtattcgtat gccgccgcca
    11521 gcagcatcat cggatgagca tcgagactcg cctgaccttt gttccgggaa gagaagggga
    11581 gcaggcagga attgccgcgc ggctgagtga caagtcccat tactcgatcg gaatcacgaa
    11641 gaaggacgga caaaccggca ttcttgtaac ggcgatggcg caaggaagcg gaacggaagt
    11701 ctttacccca attgaggagc aggttcccgt ccggctctcg atccgttcgg acgagaagga
    11761 atacgagctt ttgtatacct tgggaggtgc gtccgcggag tgggtcagcc taaggacctt
    11821 gccggtggaa gccttaacgc cggatgcgga cggagctttt acgggcgtat gtcttggcat
    11881 gtttgcagca ggaacggagg aaggccattc ggctcccgct tattacgatt acttcgagta
    11941 tcgtcccgag agcggttga
//
