LOCUS       NC_008261              13669 bp    DNA     linear   CON 17-FEB-2020
DEFINITION  Clostridium perfringens ATCC 13124, complete sequence.
ACCESSION   NC_008261
VERSION     NC_008261.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604008
            Assembly: GCF_000013285.1
KEYWORDS    RefSeq.
SOURCE      Clostridium perfringens ATCC 13124
  ORGANISM  Clostridium perfringens ATCC 13124
            Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Myers,G.S., Rasko,D.A., Cheung,J.K., Ravel,J., Seshadri,R.,
            DeBoy,R.T., Ren,Q., Varga,J., Awad,M.M., Brinkac,L.M.,
            Daugherty,S.C., Haft,D.H., Dodson,R.J., Madupu,R., Nelson,W.C.,
            Rosovitz,M.J., Sullivan,S.A., Khouri,H., Dimitrov,G.I.,
            Watkins,K.L., Mulligan,S., Benton,J., Radune,D., Fisher,D.J.,
            Atkins,H.S., Hiscox,T., Jost,B.H., Billington,S.J., Songer,J.G.,
            McClane,B.A., Titball,R.W., Rood,J.I., Melville,S.B. and
            Paulsen,I.T.
  TITLE     Skewed genomic variability in strains of the toxigenic bacterial
            pathogen, Clostridium perfringens
  JOURNAL   Genome Res. 16 (8), 1031-1040 (2006)
   PUBMED   16825665
REFERENCE   2  (bases 1 to 13669)
  AUTHORS   Shrivastava,S., Brinkac,L.M., Dodson,R.J., Harkins,D.M., Durkin,A.S.
            and Sutton,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2009) J. Craig Venter Institute, 9704 Medical
            Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   3  (bases 1 to 13669)
  AUTHORS   Myers,G.S., Rasko,D.A., Cheung,J.K., Ravel,J., Seshadri,R.,
            DeBoy,R.T., Ren,Q., Varga,J., Awad,M.M., Brinkac,L.M.,
            Daugherty,S.C., Haft,D.H., Dodson,R.J., Madupu,R., Nelson,W.C.,
            Rosovitz,M., Sullivan,S.A., Khouri,H.M., Dimitrov,G., Watkins,K.L.,
            Mulligan,S., Benton,J.L., Fisher,D.J., Atkins,H.S., Hiscox,T.,
            Titball,R.W., Rood,J.I., Melville,S. and Paulsen,I.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) The Institute for Genomic Research, 9712
            Medical Center Dr, Rockville, MD 20850, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2020 13:38:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,949
            CDSs (total)                      :: 2,828
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 17 of 32
            Pseudo Genes (incomplete)         :: 10 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            CP000246.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Clostridium perfringens ATCC 13124"
                     /mol_type="genomic DNA"
                     /strain="ATCC 13124"
                     /culture_collection="ATCC:13124"
                     /type_material="type strain of Clostridium perfringens"
                     /db_xref="taxon:195103"
     gene            1..3012
                     /locus_tag="CPF_RS01980"
                     /old_locus_tag="CPF_0394"
                     /db_xref="GeneID:29572482"
     CDS             1..3012
                     /locus_tag="CPF_RS01980"
                     /old_locus_tag="CPF_0394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL8"
                     /protein_id="WP_003455341.1"
                     /db_xref="GeneID:29572482"
                     /translation="MKESRKKLNRLISTAIVSCMVMGASYENVLALENKSQNNSEKVVK
                     YDNEAEYIKNIRLRWKEDLVGNSSLDTSNATISKKIISYTNNTDKLVAKLNMDPNAQWL
                     WEDLKDYKQNPARITSMFNNLVTMSMAYSLPNNKYYKNEDLKNKIIYSLDWINKNAYNE
                     NIDQYGNWWDWMIGIPARLNNIVVLMYDDLTEEQVKNYMNAIQKFLPSIEPGSKYHTGA
                     NLADVCMNKLLQGVNENDPEKIKEASEDIVGVFDYVTSGDGFYKDGSYLQHGMVAYTGS
                     YGNVLIEKISNIMFLLEKTPWSIKSESKDNVYKWIFESFNPIIYKGYVMDMVRGRAISR
                     YNANGYLQASGIIEGMIKIGMISDGDKASEINALVKKWATEAKSVLDFGARFKSINVIG
                     EFYGIMNNDNIKPLEEGDKHYALNSMDKTVHKRENFALGISRSSSRISKYEFMNKENLT
                     PWFQGDGMTYLFNNDLNQFSGNFWATVDPYRMPGTTVDTRKREPKEILPGLDPGASQQN
                     EIYYELGKSNWSGGSKLGAYGVAGMEIDNKYDSLKAKKSWFMFDDEIVALGSGITNPED
                     FETETIVENRKIKSDGSNKFIVDGKERVSKLKEKDKVDNAKWAYLEGNVSGSNIGYYFP
                     EGANINLIKDEREGNWINVNSSKPEADKVVKDNYLTMYIDHGKAIKDQKYSYVLLPNKT
                     EDKVKEYSENPNVEIIQNDDVAHSVKHKKLNIEAANFWKEGKNTAGNITSTGKSSIIIK
                     ENKDNTLSIAVSDPTFLEKNLSIEINKPAMEVIKSDKRISNINLENGKIKFDANTENLS
                     GAPLELLVKLGDKNNGNNENNNEIKNEAPVIEGEDANLFVGDKWDKSLHKLKAKDKEDG
                     DLTKNIKIKDNQIPLNDQFEVTKPGTYPVTFEVSDNNGKKAEKKLNVLVKEKEENKPEN
                     KPENQENKPEIKPEDQENQSTKPESEENKGENPQANNNTEKLPNTGGASNLSLGAIGVL
                     LATVGTMFTKKRKK"
     gene            3119..3949
                     /gene="kduI"
                     /locus_tag="CPF_RS01985"
                     /old_locus_tag="CPF_0395"
                     /db_xref="GeneID:29572481"
     CDS             3119..3949
                     /gene="kduI"
                     /locus_tag="CPF_RS01985"
                     /old_locus_tag="CPF_0395"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="WP_003455501.1"
                     /db_xref="GeneID:29572481"
                     /translation="MRNRYANNPKDSKRYDTEELRENYLVKDIFKDDQIELVYSHVDRI
                     IFGGIKPVYKELKLEAGKEMGVDYFLERRELGIINIGGKAIVTIDGTEYELKEKDGLYV
                     GKGNKEVSFKSVNPEEPAKLYVNSVPAHKEYETVKIDIEKANPVRLGDNKTLNKRTIYQ
                     YVHPNVCESCQLLMGLTMLEPGNAWNTMPCHTHERRMEVYFYFDMDEDTRVFHLMGEPT
                     ETRHLVVKNEECVISPSWSIHSGVGTSNYTFIWGMCGENKTFDDMDHISMDILR"
     gene            3965..4741
                     /gene="kduD"
                     /locus_tag="CPF_RS01990"
                     /old_locus_tag="CPF_0396"
                     /db_xref="GeneID:29572479"
     CDS             3965..4741
                     /gene="kduD"
                     /locus_tag="CPF_RS01990"
                     /old_locus_tag="CPF_0396"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="WP_003455308.1"
                     /db_xref="GeneID:29572479"
                     /translation="MNMEKFSLDFFKLDGKVAIVTGGNTGLGMAYVEALAAAGADVLVT
                     TFDNNTEEVKNIVESLGRKIVFVQGDLTKKETRDKVVSTCLEEFGKIDILVNNAGTIRR
                     APLLEYKDEDWQAVMDINLNSVYYLSQAVAKVMAKQGYGKIINIASMLSFQGGKFVPPY
                     TASKHGVAGITKAFANELADLNIQVNAIAPGYIKTANTAPIRADKARNQEILSRIPAGR
                     WGEVSDLMGTVVFLSSKASDYLNGHILAIDGGWLVR"
     gene            4738..5379
                     /gene="eda"
                     /locus_tag="CPF_RS01995"
                     /old_locus_tag="CPF_0397"
                     /db_xref="GeneID:29572478"
     CDS             4738..5379
                     /gene="eda"
                     /locus_tag="CPF_RS01995"
                     /old_locus_tag="CPF_0397"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="WP_011590177.1"
                     /db_xref="GeneID:29572478"
                     /translation="MIKKIKTLTNLINEAFVLVLRTNTKDEGIETAKAAIKGGCNIIEV
                     TFTIPNADKVIEELIKDKNEEVVIGAGTVLDAETARIAILAGAEFIVSPSFDKETATLC
                     NRYGIPYIPGCFTPREIKEARECGSDVIKLFPGSAFKPTIVKDLKAPIKGIAVMASGGV
                     SFENMDEWFNNSCDIVSIGSAIIKLKDPAKIEAETRKYIERVKKLRMQRG"
     gene            5383..6402
                     /locus_tag="CPF_RS02000"
                     /old_locus_tag="CPF_0398"
                     /db_xref="GeneID:29572477"
     CDS             5383..6402
                     /locus_tag="CPF_RS02000"
                     /old_locus_tag="CPF_0398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|PfkB"
                     /protein_id="WP_003455275.1"
                     /db_xref="GeneID:29572477"
                     /translation="MKKVLVLGEMLMRLTPPNNNKIGQANSFNVCFGGAEVNVAVGLSN
                     LGIETRVLTALPDNEIGNFAKSFLKSQYVDCDFIISKGDRLGLYYYEEGCSARKAKVVY
                     DRKHSSITELQYEDIDVESLFKDVELLHISGITFGLSEEVSEVALKLVNEAKKRGIKIS
                     VDLNYRSKLFESYEEFVRIMKPVVQDAYLCFGWLNREDEPFKVLDASTTELSDEDFIER
                     FRYTIEDLKVKNIVTTLRSGTPYNYHTLQGVFYDGERVYKSSKYDFSMISRIGGGDAFA
                     AGVIRTLIENKGEDLSHVIEFGTAAAVLKQTQLGDVSISSKEEVLDLINNKNLGSVNR"
     gene            6567..7397
                     /gene="kduI"
                     /locus_tag="CPF_RS02005"
                     /old_locus_tag="CPF_0399"
                     /db_xref="GeneID:29572476"
     CDS             6567..7397
                     /gene="kduI"
                     /locus_tag="CPF_RS02005"
                     /old_locus_tag="CPF_0399"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="WP_003455313.1"
                     /db_xref="GeneID:29572476"
                     /translation="MRTRYANNPRDSKRYDTEELRENYLVEDIFKDDQIELVYSHVDRI
                     IFGGIKPVYKELKLEAGKEMGVDYFLERRELGIINIGGKAIVTIDGTEYELKEKDGLYV
                     GKGNKEVSFKSVNPEEPAKLYVNSVPAHKEYETVKIDIEKANPVRLGDNKTLNKRTIYQ
                     YVHPNVCESCQLLMGLTMLEPGNAWNTMPCHTHERRMEVYFYFDMDEDTRVFHLMGEPT
                     ETRHLVVKNEECVISPSWSIHSGVGTSNYTFIWGMCGENKTFDDMDNIPMDVLR"
     gene            7414..8604
                     /locus_tag="CPF_RS02010"
                     /old_locus_tag="CPF_0400"
                     /db_xref="GeneID:29572475"
     CDS             7414..8604
                     /locus_tag="CPF_RS02010"
                     /old_locus_tag="CPF_0400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013853183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH88"
                     /protein_id="WP_003455311.1"
                     /db_xref="GeneID:29572475"
                     /translation="MIKEIRVEEIAKKDEFLKTKLLTRAEVKNAIDLVIKQIDANMEYF
                     KEKFPSSATKNNQYGIIENIEWTDGFWTGLLWLAYEYTGDEKYRELADKNVASFKNRVE
                     KDIELDHHDLGFLYSLATVSGYKLTGSEDAREASIKAANKLISRYQEKGEFIQAWGELG
                     SKDHYRFIIDCLLNIPLLYWASDETGDAKYRNIANKHFVTSCNNVIRDDASAFHTFYMD
                     NETGKPLRGVTRQGYSDDSAWARGQAWGVYGIPLNYRYTRNESCFNLYEGMTNYFLNRL
                     PKDNVCYWDLIFNDGDDHSKDSSAAAIAVCGMHEMNKYLPEVDENKEVYKYAMHNILRS
                     LMENYMNPEIEPGKPVLLHGVYSWHSGKGVDEGNIWGDYFFLEALIRFYKDWNLYW"
     gene            8621..9109
                     /locus_tag="CPF_RS02015"
                     /old_locus_tag="CPF_0401"
                     /db_xref="GeneID:29572474"
     CDS             8621..9109
                     /locus_tag="CPF_RS02015"
                     /old_locus_tag="CPF_0401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR76|4.A.6.1.14"
                     /protein_id="WP_003455664.1"
                     /db_xref="GeneID:29572474"
                     /translation="MGVNIEMMRIDERLIHGQGQMWLNSLGVNTVIVANDIASEDKIQQ
                     TLMKTVVPKSIAMRFFSIQKTCELIHKASPQQKIFLVCKTPEDALKLIAGGVPVKEINI
                     GNIHNAEGKEQVTRSIFLGKEDKDALRELSEKYNVKFNTKTTPRGDDGAMQVDITKYL"
     gene            9157..9948
                     /locus_tag="CPF_RS02020"
                     /old_locus_tag="CPF_0402"
                     /db_xref="GeneID:29572473"
     CDS             9157..9948
                     /locus_tag="CPF_RS02020"
                     /old_locus_tag="CPF_0402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR75|4.A.6.1.14"
                     /protein_id="WP_003455334.1"
                     /db_xref="GeneID:29572473"
                     /translation="MEITLIQCILIGLWTAFCLAGMLLGLYTNRCIILAFGVGIILGDI
                     PTALAMGAVSEIAFMGFGVGAGGTVPPNPMGPGIIGTLMAITMKGQGMDPATALALSFP
                     FAVAFQFLITATYTFATGLTQFANKGIEEGNWIKFKIGANATIWVFAIVGFIIGFAGAF
                     STTGLQAAVEMIPAWLIGGLSVAGKMLPAIGFAMILSIMAKTELIPFVLLGYVCIGYLN
                     LPVMGVAFVGAIFALIEHFRGNNSNNSSDSNDEEVVFEGGI"
     gene            9917..10747
                     /locus_tag="CPF_RS02025"
                     /old_locus_tag="CPF_0403"
                     /db_xref="GeneID:29572472"
     CDS             9917..10747
                     /locus_tag="CPF_RS02025"
                     /old_locus_tag="CPF_0403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR74|4.A.6.1.14"
                     /protein_id="WP_162467389.1"
                     /db_xref="GeneID:29572472"
                     /translation="MRRLYLKVESRVLTKSDYIKTSLRAFFLQNGFNYGNYQGLGYANI
                     LYPALKKIYRNNEEGFKKALGENVEFFNSNPHFLPFITSLHLVMLDSGRSSEEARTIKL
                     ALMGPLAGIGDSLSQFCLAPLFSTIAASLAQDGLIAGPILFFVAMNAILLTIKLLTGIY
                     GYKLGTSIIDVLSEKMAQISAVASMIGVTVISGLAVSFTKIQIAYKYTAQMPDGAEKVV
                     SIQEMLDKIAPALLPALYTILIFYLIKKRKWTTYHLVILTIVVGILGSVLKILA"
     gene            10817..11230
                     /locus_tag="CPF_RS02030"
                     /old_locus_tag="CPF_0404"
                     /db_xref="GeneID:29572471"
     CDS             10817..11230
                     /locus_tag="CPF_RS02030"
                     /old_locus_tag="CPF_0404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DR78|4.A.6.1.14"
                     /protein_id="WP_003455482.1"
                     /db_xref="GeneID:29572471"
                     /translation="MKILVVGHGEYSTGIKSVIKLLTGVEENIDAINLNGELTHDEFTV
                     KVKNYIEDNNDLIVFADITGGAPFQITSREILLNEKSENQYVVGGVSVACILEVVMNTL
                     VLENNENHRGVIEEALNKLPEMSSVICRKDLVG"
     gene            11233..11511
                     /gene="yajC"
                     /locus_tag="CPF_RS02035"
                     /old_locus_tag="CPF_0405"
                     /db_xref="GeneID:29572470"
     CDS             11233..11511
                     /gene="yajC"
                     /locus_tag="CPF_RS02035"
                     /old_locus_tag="CPF_0405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="WP_003455254.1"
                     /db_xref="GeneID:29572470"
                     /translation="MNDRVLWLIILVLATYTIIISFVSPMIHKKRMKEQEKARDIYLSK
                     LKPGDKVIIISGIYGIIKGINNNIVKLEISKGVIIEIDKESIMGVLN"
     gene            11651..13669
                     /locus_tag="CPF_RS02040"
                     /old_locus_tag="CPF_0406"
                     /db_xref="GeneID:29572469"
     CDS             11651..13669
                     /locus_tag="CPF_RS02040"
                     /old_locus_tag="CPF_0406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL12_1"
                     /protein_id="WP_003455377.1"
                     /db_xref="GeneID:29572469"
                     /translation="MSNYSNKIEGLKAKVNEYMQVYDAEFVRYYINHNCKNEVDKKLIG
                     SNLILNNSFIFDDEWDMEQCKIPYLNRDLDWNFTPNGDEEWVFMLNRHEYFEKLIAAYY
                     FSNDEKYLDKLKELIFNWIEKNEIKECGGPTIRTIDTGIRCFSWMKSLLHLIHENKLED
                     EEILKIISSIKEQLEYLKKSYIDKYVLSNWGVLQTTAIITCSLWLKEFIEDEELYKWAL
                     EELYREINLQVLEDGSHWEQSVMYHIEVLNCSMATIHYVKYFNVDLDEEFLEKIHSMAK
                     YLVYCGDSNSIQVAQGDSDRSDIRDVLLRASILFNDPHLKFRAYETMDLTSILLFGRDG
                     FLKYTNMDAEEITKLNKSFIDSGNIYIRSGWDKEASFTYLQNGTLGSGHGHSDLCHFSI
                     HYGGEPFLIDSGRYTYVESDLLREYLKSVKAHNVSVIDDSPFAIPKNSWKYNKYPDVMK
                     NYFNEKDNIAYAEMAYLATLSDGTPYTVIRKVLVISPDIWVIVNDIRCSGKHICKNYYN
                     LDYKVKVIKEEGYFRCVNKESEIKIYNNNVDKKYIENTLISTNYNSINNSKKIVTQGTF
                     ENNFVNYDIILGQNLKSIEIKDPSIVQYNSKEKIDTSVAITKEFVINENESYTVIIFNK
                     ETFKGAKVYMYDDLALYGKVIVVHRVKDKREIIRLKA"
ORIGIN
        1 atgaaagaat ctagaaaaaa acttaacaga cttatatcaa cggcaattgt aagttgtatg
       61 gttatgggag cttcctatga gaatgttttg gcacttgaaa ataagagtca aaataattct
      121 gaaaaggtag tgaaatatga caacgaagct gagtatatta aaaacattag attaagatgg
      181 aaggaggatt tagtaggtaa ttcttcttta gatacaagta atgctacaat atctaagaag
      241 ataataagtt atacaaataa tacagacaaa ttagtagcaa aacttaatat ggatcctaac
      301 gctcaatggc tttgggaaga tttaaaagat tataaacaaa atccagctag aataacatct
      361 atgttcaata acttagtaac aatgtctatg gcatatagct tacctaataa taagtattac
      421 aaaaatgaag atttgaaaaa taagataata tattccttag actggatcaa taaaaatgct
      481 tacaatgaga atattgacca gtatggcaac tggtgggatt ggatgattgg aataccagca
      541 agattaaata atattgttgt tttaatgtat gatgatttaa ctgaagaaca agttaaaaat
      601 tatatgaatg caatacaaaa attcttacca agtattgagc ctggaagtaa atatcataca
      661 ggagcaaatt tagcagatgt atgtatgaat aagttattgc aaggtgtcaa tgagaatgat
      721 ccagaaaaaa ttaaagaagc atcagaggat atagttggag tttttgatta tgtaactagt
      781 ggggatggat tctataagga tggttcatat cttcaacatg gaatggtagc atacacaggt
      841 tcatatggaa atgttcttat tgagaaaata tctaatataa tgtttttatt agaaaaaact
      901 ccatggtcaa taaaatctga aagtaaagat aacgtttata agtggatttt tgagagtttc
      961 aatccaatta tatataaagg atacgttatg gacatggtta gaggaagagc aatatcaaga
     1021 tataatgcta atggatactt acaagcatct ggaattattg aaggtatgat taaaattgga
     1081 atgatttctg atggagataa ggctagtgaa ataaatgctc tagttaaaaa atgggctaca
     1141 gaagctaaga gtgtattaga ttttggagca agatttaagt caattaatgt aataggtgaa
     1201 ttctatggaa ttatgaataa tgacaatata aaacctttag aagaaggtga taagcattat
     1261 gcattaaata gtatggacaa gactgttcat aaaagagaga acttcgcttt aggtatatca
     1321 agaagttcaa gtagaattag taaatatgaa ttcatgaaca aggaaaattt aacaccatgg
     1381 ttccaagggg atggaatgac ttatttattc aataatgatt taaatcaatt ctcaggaaat
     1441 ttttgggcta cagtagatcc atatagaatg ccaggtacaa ctgtagacac tagaaaaaga
     1501 gaaccaaaag aaatattacc agggttagat ccaggagcat cacaacaaaa tgaaatttat
     1561 tatgaattag gaaagagtaa ttggtctggt ggaagtaagt taggagctta cggagtagct
     1621 ggaatggaaa tagataataa gtacgattcc ttaaaagcta agaaatcttg gtttatgttt
     1681 gatgatgaaa tagttgcctt aggttcagga ataactaatc cagaagattt tgaaactgaa
     1741 acaatagttg aaaatagaaa gataaaaagt gatggatcaa ataaatttat agtagatgga
     1801 aaagaaagag taagtaaatt aaaagaaaaa gataaagttg ataatgcaaa atgggcttac
     1861 ttagaaggaa atgtaagtgg atcaaatata ggatattatt tcccagaggg agcaaatatt
     1921 aatttaataa aagatgaaag agaaggtaat tggattaatg taaactcttc taaaccagaa
     1981 gcagataagg tggttaaaga taattactta actatgtata tagatcatgg aaaagctata
     2041 aaagatcaaa aatatagtta tgtattatta ccaaataaga ctgaggataa ggtaaaagaa
     2101 tattctgaga atccaaatgt tgaaattatt caaaatgatg atgtagctca tagtgttaag
     2161 cataaaaaat taaatattga agcagctaac ttctggaaag agggaaaaaa tactgctgga
     2221 aatataacat caacaggaaa atcatctata ataataaaag aaaataaaga taatacctta
     2281 agcatagctg tgtcagatcc aactttctta gaaaaaaatc tttctataga aataaataaa
     2341 ccagcaatgg aagtaataaa atcagataaa agaatatcaa atataaattt agaaaatgga
     2401 aaaataaaat ttgatgcaaa cacagaaaac ctttcagggg cacctttaga gcttcttgta
     2461 aaattaggcg acaaaaataa tggaaacaat gaaaataata atgaaattaa aaatgaagct
     2521 cctgtaatag aaggagaaga tgctaattta tttgtaggag ataagtggga taaatctctt
     2581 cacaaactta aggctaaaga taaggaggat ggagatttaa ctaaaaatat taagattaag
     2641 gataatcaaa ttcctttaaa tgatcaattt gaagttacaa agcctgggac atatccagtt
     2701 acttttgaag taagtgataa taatgggaaa aaagcagaga aaaagcttaa tgttttagtt
     2761 aaagaaaagg aagagaacaa gccagaaaat aaaccggaaa atcaagagaa taaaccagag
     2821 attaaaccag aggatcaaga aaatcaatca acaaaaccag aaagtgaaga aaataaaggg
     2881 gaaaatccac aagcaaataa taatactgaa aagctaccta acactggagg agcaagtaat
     2941 ctaagccttg gagcaatagg tgttcttcta gctactgttg gaacaatgtt tactaagaaa
     3001 agaaaaaaat aatttatagt gattaaaggc gttgacaata taagtaagga attatataat
     3061 taacgtaagg ttggtaaacc agtttatata aaacgtttta acaataggag gagagtttat
     3121 gaggaataga tatgctaata acccaaagga ttcaaaaaga tatgatactg aggaattaag
     3181 agaaaattac ttagttaaag acatatttaa agatgatcaa atagaacttg tatatagtca
     3241 tgtagataga ataattttcg gaggaataaa accggtttac aaagaattaa aattagaagc
     3301 cggcaaagag atgggggttg attatttctt agaaagaaga gagctaggaa taatcaatat
     3361 aggcggaaaa gctatcgtaa ctattgacgg aactgagtat gaattaaaag aaaaagatgg
     3421 tttatatgtt ggaaaaggaa ataaagaagt aagctttaaa tcggtaaatc cagaagaacc
     3481 agcaaagtta tatgttaact cagtaccagc tcataaggaa tatgaaaccg ttaagattga
     3541 tatagaaaag gctaatccag ttagattagg agacaacaaa acattaaaca agagaacaat
     3601 atatcaatat gtacatccaa atgtatgtga aagttgtcaa ttattaatgg gattaacaat
     3661 gttagaacca ggaaatgctt ggaacacaat gccttgccat acacatgaaa gaagaatgga
     3721 agtttacttc tacttcgata tggacgaaga cacaagagta ttccacttaa tgggagaacc
     3781 tacagaaaca agacatttag ttgttaagaa tgaagagtgt gtaatatcac caagttggtc
     3841 aatacattct ggagtaggta caagcaatta cacatttata tggggtatgt gtggagaaaa
     3901 taaaactttt gatgatatgg atcatatctc aatggatatt ttaagataaa aggagaggat
     3961 taagatgaat atggagaaat tttctttaga cttttttaaa ttagatggta aagtagctat
     4021 agtaactggt ggaaacactg gattaggaat ggcttatgta gaagctcttg cagcagcagg
     4081 tgctgatgtt ttagtaacaa cttttgataa caatactgaa gaggtaaaaa atattgttga
     4141 atcattagga agaaaaattg tatttgtaca aggagattta actaaaaaag aaacaagaga
     4201 taaggttgta tcaacttgtt tagaggaatt tggaaaaata gatatattag taaataatgc
     4261 aggaacaata agaagagcac cattacttga gtacaaagat gaagattggc aagctgtaat
     4321 ggatataaac ttaaattcag tttactattt atcacaagca gttgctaaag taatggctaa
     4381 gcaaggatat ggtaagatta taaacatagc atctatgtta tcattccaag gtggaaaatt
     4441 tgttcctcca tacactgcaa gtaaacatgg agttgcagga ataacaaagg catttgctaa
     4501 tgaattagca gatttaaata tacaagtaaa tgctattgca cctggatata taaaaactgc
     4561 taacactgca ccaattagag cagataaagc tagaaaccaa gaaattcttt caagaatacc
     4621 agcaggtaga tggggagaag tttcagattt aatgggaaca gttgtattct tatcaagtaa
     4681 agcatctgat tacttaaatg gacacatatt agctatagac ggtgggtggt tagtgagatg
     4741 ataaagaaaa taaaaacatt aacaaattta ataaatgaag cttttgtatt ggttcttaga
     4801 accaatacaa aagatgaagg tatagaaact gcaaaggctg caataaaggg aggatgtaat
     4861 attatagagg ttacatttac tattccaaat gcagataagg tgattgaaga gcttattaaa
     4921 gataagaatg aagaagtagt tattggagca ggtactgttt tagatgctga aacagctaga
     4981 attgcaatac ttgcaggtgc agaatttatt gttagtccaa gctttgataa agaaacagct
     5041 acactatgta atagatatgg aattccttat attccaggat gttttacacc aagagaaatt
     5101 aaggaagcta gagaatgtgg atcagacgta ataaaattat tcccaggatc agctttcaag
     5161 ccaactatag taaaagattt aaaggctcct ataaaaggta ttgcagtaat ggcatctggt
     5221 ggagttagct ttgaaaatat ggatgaatgg tttaataata gttgcgatat agttagtatt
     5281 ggaagtgcta ttataaaact aaaagatcct gcaaaaatag aggcagaaac tagaaaatat
     5341 atagagcgtg ttaaaaaatt acgtatgcaa cgaggttaga gaatgaagaa agttttagta
     5401 ttgggagaaa tgttaatgag gctaactcct ccaaacaaca ataaaatagg acaagcaaat
     5461 agctttaatg tgtgttttgg tggagccgaa gtaaatgtgg cagtaggatt aagtaattta
     5521 ggaattgaaa ctagagtatt aacagcttta cctgataatg aaattggaaa ctttgcgaaa
     5581 tcttttttga aatcacaata tgttgattgt gatttcataa tttcaaaagg ggataggctt
     5641 ggactatact attatgagga aggatgttct gctagaaaag ctaaggttgt ttatgatagg
     5701 aaacattctt caattactga gttacaatat gaagatatag atgttgaaag cttatttaaa
     5761 gatgttgagt tattacatat atctggaata acttttggtt tatctgaaga agtaagtgaa
     5821 gttgccctta aattagttaa tgaggctaaa aaaagaggta taaagataag tgttgattta
     5881 aactatagat caaaattgtt tgaaagttat gaggagtttg ttagaataat gaagcctgta
     5941 gtccaagatg cctatttatg ctttggttgg ttaaatagag aggatgagcc ttttaaggtt
     6001 ttagatgcat caactacaga actttcagat gaggatttta ttgaaagatt tagatataca
     6061 attgaagatt taaaagttaa aaatatagta actaccttaa ggtctggaac accatataat
     6121 tatcatactt tacaaggcgt gttttatgat ggggaaagag tatataaatc ctcaaaatat
     6181 gattttagta tgatttcaag aattggtgga ggagatgctt ttgccgcagg ggttataaga
     6241 actctaattg aaaataaagg ggaggactta tcccatgtta tagagtttgg aacagctgct
     6301 gcagtattaa aacaaactca gcttggagat gtaagtattt cttcaaagga agaggtttta
     6361 gatctaatta ataataagaa tttaggtagt gtaaatagat aaattttcta taaatgtaat
     6421 taaatattat tgaagtataa atgttgtaaa aaaactataa aaagaaaaag aaaacgttga
     6481 caataatatt ttaggataat ataataaaaa tataagttag taaaacagtt tatgtaaaac
     6541 gttttaataa aaggtgggag gaaattatga gaactagata tgctaataat ccaagggact
     6601 caaaaagata tgatactgag gaattaagag aaaattactt agttgaagac atatttaaag
     6661 atgatcaaat agaacttgta tatagtcatg tagatagaat aattttcgga ggaataaaac
     6721 cggtttataa agagcttaaa ttagaagctg gtaaagagat gggtgttgat tatttcttag
     6781 aaagaagaga gctaggaata attaatatag gcggaaaagc tatcgtaact attgatggaa
     6841 ctgagtatga attaaaagaa aaagatggtt tgtatgttgg aaaaggaaat aaagaagtaa
     6901 gctttaaatc agtaaatcca gaagaaccag caaagttata tgttaactca gtaccagctc
     6961 ataaggaata tgaaaccgtt aagattgata tagaaaaggc taatccagtt agattaggag
     7021 acaacaaaac attaaacaag agaacaatat atcaatatgt acatccaaat gtatgtgaaa
     7081 gttgtcaatt attaatggga ctaacaatgt tagaaccagg aaatgcttgg aacacaatgc
     7141 cttgccatac acatgaaaga agaatggaag tttacttcta cttcgatatg gacgaagaca
     7201 caagagtatt ccacctaatg ggagaaccta cagaaacaag acatttagtt gttaagaatg
     7261 aagagtgtgt aatatcacca agttggtcaa tacattctgg agtaggtaca agcaattaca
     7321 catttatatg gggtatgtgt ggagaaaata aaacttttga tgatatggat aatattccaa
     7381 tggatgtctt aagataattt gagaggggta tttatgatta aggaaataag agttgaagag
     7441 attgcaaaga aagatgaatt tctgaaaact aagttattaa ctagagctga ggttaaaaat
     7501 gctattgatt tagttataaa acaaatagat gctaatatgg aatatttcaa agaaaaattt
     7561 ccatcatcag caacaaaaaa taatcagtat ggaattatag aaaatataga gtggactgat
     7621 ggattctgga caggcttatt atggcttgct tatgagtaca caggagatga aaaatataga
     7681 gagttagcag ataaaaatgt tgcatctttt aaaaacagag tggaaaaaga tatagaatta
     7741 gatcatcatg atttaggatt cttatattca ttagctacag taagtggtta taaattaaca
     7801 ggctctgagg atgcaagaga agcttcaata aaagctgcaa ataaacttat ttctagatac
     7861 caagaaaaag gtgagtttat tcaagcttgg ggagagctag gtagcaaaga tcactacaga
     7921 tttataatag attgtttatt aaatatacca ttattatact gggctagtga tgaaacagga
     7981 gatgcaaagt atagaaacat agcaaataaa cattttgtta catcttgtaa taatgttata
     8041 agagatgatg catctgcatt ccatacattc tatatggata atgaaactgg aaaaccttta
     8101 agaggagtta ctagacaagg atatagtgat gattcagctt gggctagagg acaagcttgg
     8161 ggagtttatg gtattccatt aaattataga tatacaagaa atgaaagttg ttttaatctt
     8221 tatgagggga tgactaatta tttcttaaat agattaccta aggataacgt ttgttattgg
     8281 gatttgattt tcaatgatgg tgatgatcat tcaaaggatt cttcagcagc agcaatagct
     8341 gtttgtggta tgcatgaaat gaataaatat ttaccagaag ttgacgaaaa taaagaagta
     8401 tataaatatg caatgcataa tattttaaga tcattaatgg aaaattatat gaatcctgaa
     8461 atagaacctg gaaaaccggt tttattacat ggagtatatt catggcattc aggaaaaggt
     8521 gtagatgaag gaaacatatg gggagattat ttcttcttag aagccttaat aagattttat
     8581 aaagattgga acctttattg gtaaaaagga gtgttgaaaa atgggcgtaa atattgaaat
     8641 gatgagaatt gatgaaagat taattcatgg acaaggacaa atgtggttaa attcattagg
     8701 agttaatact gtaatagttg caaacgatat tgctagtgaa gataaaatac aacaaacatt
     8761 aatgaaaact gttgtaccaa aatcaatagc tatgagattt ttctcaatac aaaaaacttg
     8821 tgagttaata cacaaagcat caccacaaca aaaaatcttc ttagtttgta aaactccaga
     8881 ggatgcatta aaactaattg caggtggagt accagttaaa gaaataaaca taggaaatat
     8941 acacaatgct gaaggtaaag agcaagttac tcgttcaatt ttcttaggaa aagaagataa
     9001 agatgcatta agagaattat cagaaaagta taatgttaag tttaatacaa aaacaacacc
     9061 tagaggtgac gatggagcaa tgcaagtaga tataactaaa tatctatagt aaataaattt
     9121 aataagggtt attagaaaaa gcggaggtaa tattatatgg aaattacatt aattcaatgt
     9181 attttaatag ggttatggac agccttttgt ttagcaggta tgttacttgg actttataca
     9241 aaccgttgta ttatcctagc ttttggagtt ggaataattt taggtgatat tccaactgca
     9301 ttagctatgg gggcggtttc agaaatagct ttcatgggct ttggagtagg agctggtgga
     9361 acagtgccac caaacccaat gggaccagga attataggta cattaatggc aattactatg
     9421 aaaggtcaag gaatggatcc agcaactgca cttgctttat catttccatt cgcagtagct
     9481 ttccaattct taattacagc aacttataca tttgcaacag gtttaactca atttgcaaat
     9541 aagggtattg aagaaggaaa ctggattaaa tttaaaatag gtgctaatgc aactatttgg
     9601 gtattcgcta tagtaggatt tattattgga tttgcaggag catttagtac aactggatta
     9661 caagcagcag tagaaatgat accagcttgg ttaataggtg gattatcagt agcgggtaaa
     9721 atgttaccag caataggatt tgcaatgata ttatcaataa tggcaaaaac agaacttata
     9781 ccatttgtat tattaggata tgtatgtata ggatacttaa acttaccagt aatgggagta
     9841 gcttttgtag gagcaatatt cgctttaata gaacatttca gaggaaataa ttctaacaat
     9901 tcaagcgata gtaatgatga ggaggttgta tttgaaggtg gaatctagag tattaaccaa
     9961 aagtgattat ataaaaacat ctcttagagc attcttttta cagaatggat ttaactatgg
    10021 taactatcaa ggattaggat atgcaaatat actatatcct gctttaaaga aaatttatag
    10081 aaataatgaa gaaggcttta agaaagcatt aggtgaaaac gtagaattct ttaactctaa
    10141 ccctcacttt cttcctttca tcacaagttt acatttagta atgcttgact caggaagatc
    10201 atctgaagag gctagaacaa taaaacttgc attaatggga cctctagcag gaataggtga
    10261 ttcactttca caattctgtt tagcaccatt attctcaaca atagcagcta gtttagctca
    10321 agatggttta atagctggac caatattatt ctttgtagct atgaacgcta tattattaac
    10381 aattaaatta ttaacaggta tatatggtta taaattagga actagtatta tagatgtttt
    10441 aagtgaaaaa atggctcaaa tctctgctgt agctagtatg atcggtgtta ctgttatatc
    10501 aggattagca gtttctttca caaaaataca aattgcttat aaatacacag ctcaaatgcc
    10561 agatggagct gaaaaagttg tatcaataca agaaatgtta gataaaatag caccagctct
    10621 tttaccagca ttatatacaa tacttatatt ctaccttata aagaaacgta aatggacaac
    10681 ttaccattta gttatcttaa caatagtagt aggtatttta ggatcagttt taaaaatttt
    10741 agcttagtaa atgtgtagag ggtagtattt taaaatacta ctctctaaaa taaattaaga
    10801 ttaggagtat taagagatga aaatattagt agtaggacat ggagaatatt caactgggat
    10861 aaaatcagta ataaaattac taactggagt agaagaaaat attgatgcaa taaatttaaa
    10921 tggagaatta actcatgatg agtttactgt taaagttaaa aactacatag aagataataa
    10981 tgatctaatt gtttttgcag atataacagg gggagcacct ttccaaataa catcaagaga
    11041 aatattatta aatgaaaaat cagaaaatca atatgttgtt ggtggagttt cagtagcttg
    11101 tattttagaa gttgttatga atacattagt attagaaaac aatgaaaatc atagaggagt
    11161 tattgaagaa gctttaaata aattacctga aatgtcatca gttatttgta gaaaggactt
    11221 agttggttaa ctatgaatga tagagtgctt tggttaataa tattagtact ggctacatat
    11281 actattataa tatcttttgt ttcaccaatg atacacaaaa aaagaatgaa agagcaagaa
    11341 aaggctagag acatttattt atcaaaatta aaaccaggag acaaggttat tattatatca
    11401 ggcatatatg ggataattaa aggaataaac aataatatag ttaaattaga aatttcaaag
    11461 ggcgttataa tagaaatcga taaagaaagt attatgggcg ttttaaatta aaagcagaat
    11521 atttaataaa aataatactt attagagtaa gtggcataaa tgccgaattt catggataat
    11581 aaaatattaa tttgaaaaga agactactta atataagtgg tctttatttt atttataagg
    11641 tggagatatt atgagtaatt attctaataa aatagaggga ttaaaagcaa aggttaatga
    11701 gtatatgcaa gtatatgatg ctgagtttgt aaggtattat ataaatcata attgtaagaa
    11761 tgaagtagat aaaaagttaa taggttctaa tttaattctt aataattctt ttatatttga
    11821 tgatgaatgg gatatggaac aatgtaaaat tccatattta aatagagatt tagattggaa
    11881 ctttactcct aatggagatg aggaatgggt atttatgctt aatagacatg aatactttga
    11941 aaaattaatt gcagcctatt attttagtaa tgatgaaaaa tatttagata agttaaagga
    12001 attaatattt aattggattg aaaagaatga aataaaggag tgtggaggac caacaataag
    12061 aacaatagat actggaataa gatgttttag ttggatgaag tctcttttac acttgattca
    12121 tgaaaataaa ttagaagatg aagaaatatt aaaaataatt tctagtataa aagaacaatt
    12181 agagtatttg aaaaaatctt atattgataa atatgttctt agcaattggg gagtattaca
    12241 gacaacagca ataattactt gctcactatg gttaaaagaa tttattgagg atgaggaatt
    12301 atataagtgg gcacttgagg aactatacag ggaaataaat cttcaagttt tagaagatgg
    12361 ttcacattgg gagcagtctg taatgtatca tattgaagta ctaaattgtt ctatggcaac
    12421 tatacactat gttaagtatt ttaatgttga tttagatgag gagttcttag aaaaaataca
    12481 ttctatggct aaatatttag tttactgtgg agattcaaat tcaattcaag ttgctcaagg
    12541 ggacagtgac agaagtgata ttagagatgt tcttcttagg gcatctatat tatttaatga
    12601 tcctcattta aaatttagag catatgaaac tatggattta accagtatat tattgtttgg
    12661 aagagatgga tttttaaaat atacaaatat ggatgcagag gaaattacaa agcttaataa
    12721 gtcctttata gactctggta atatttatat aagaagtgga tgggacaaag aggcaagttt
    12781 tacttattta caaaatggta ctcttggaag tggacatgga cattcagatt tatgccattt
    12841 ttcaattcat tatggtggag aaccattttt aatagattca ggtaggtata cctatgttga
    12901 aagtgatctt ttaagggaat atttaaaatc tgttaaggct cataatgtat ctgtaattga
    12961 tgattctcct tttgctattc ctaagaattc atggaaatat aataaatatc cagatgttat
    13021 gaaaaattat tttaatgaaa aagataatat agcttatgct gaaatggctt atttagcaac
    13081 tttaagtgat ggaacaccat atacggttat tagaaaggtg ttagttatat ctccagatat
    13141 atgggtaata gttaatgata taagatgtag tggaaagcat atatgcaaaa attattataa
    13201 tttagattat aaggttaagg tgattaaaga agagggttat tttagatgtg taaataaaga
    13261 aagtgaaatt aagatttata ataacaatgt agataagaaa tatatagaaa atactttaat
    13321 ttcaacaaat tataatagta taaataatag taaaaagata gtaacacaag gtacttttga
    13381 aaataatttt gtgaactatg atataatttt agggcagaat ttaaaaagta tagaaataaa
    13441 ggatcctagt attgtacaat ataattcaaa agaaaagatt gatacaagtg tggcaataac
    13501 taaagaattt gtaataaacg aaaatgagag ttatactgtt ataatattta ataaagaaac
    13561 atttaaaggg gctaaggtat atatgtatga cgatttggct ttatatggta aggttattgt
    13621 tgtgcataga gttaaagaca agagagaaat aattcgtttg aaagcataa
//
