LOCUS       AY526725                6510 bp    DNA     linear   BCT 07-JUN-2004
DEFINITION  Cellvibrio mixtus Unk1 (unk1), Unk2 (unk2), Man5A (man5A), and
            Aga27A (aga27A) genes, complete cds.
ACCESSION   AY526725
VERSION     AY526725.1
KEYWORDS    .
SOURCE      Cellvibrio mixtus
  ORGANISM  Cellvibrio mixtus
            Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae; Cellvibrio.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Dias,F.M., Vincent,F., Pell,G., Prates,J.A., Centeno,M.S.,
            Tailford,L.E., Ferreira,L.M., Fontes,C.M., Davies,G.J. and
            Gilbert,H.J.
  TITLE     Insights into the molecular determinants of substrate specificity in
            glycoside hydrolase family 5 revealed by the crystal structure and
            kinetics of Cellvibrio mixtus mannosidase 5A
  JOURNAL   J. Biol. Chem. 279 (24), 25517-25526 (2004)
   PUBMED   15014076
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Centeno,M.S., Dias,F.M., Guerreiro,C.I., Ferreira,L.M. and
            Fontes,C.M.
  TITLE     Genes encoding galactomannan degrading enzymes are clustered in
            Cellvibrio mixtus genome
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6510)
  AUTHORS   Centeno,M.S., Dias,F.M., Guerreiro,C.I., Ferreira,L.M. and
            Fontes,C.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2004) Centro Interdisciplinar de Investigacao em
            Sanidade Animal, Faculdade de Medicina Veterinaria, Rua Prof. Cid
            dos Santos 1300-477 Lisboa, Portugal
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Cellvibrio mixtus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:39650"
     gene            112..1293
                     /gene="unk1"
     CDS             112..1293
                     /gene="unk1"
                     /codon_start=1
                     /transl_table=11
                     /product="GH130"
                     /protein_id="AAS19693.1"
                     /translation="MSSFKEKAKALLQQHETLITKKNAVKRDGNGIYDCYENPILTAEH
                     APVFWRYDLNEKTNPHLMERQGINAAFNSGAMYWNGKYILAVRVEGVDRKSFFAIAESP
                     NGIDNFRFWDYPITMPETDRPDTNVYDMRLTNHEDGYIYGLFCTERKDLNHPNDTSAAE
                     AQCGIARTKDLITWERLPDLVTYSGQQRNVVLHPEFIDGKYGLFTRPQDGFISVGAGGG
                     IGWGLVDDMTRAEVKSEVIVDGKVYHTIKEVKNGQGPAPIKTAEGWLNLAHGVRNTAAG
                     LRYVLYMFMTELERPWVVTHRPAGHFIAPHGAERVGDVSNVAFSNGWIANEKNEVFIYY
                     ASSDTRMHVATSTVEKLIDYCKNTPEDGLRSAASVAVRNELISANLQVMKDLV"
     gene            1297..2529
                     /gene="unk2"
     CDS             1297..2529
                     /gene="unk2"
                     /codon_start=1
                     /transl_table=11
                     /product="Unk2"
                     /protein_id="AAS19694.1"
                     /translation="MNAAMLPSSVIVLQQEFRAELSAIADWWASHTIDQQQGGFYGEVG
                     ADNLPVPSASKGIILNARILWFFSETAQQINNPVYRDCAERAYHYLCANFFDDVHGGVY
                     WELDAAGAPINTKKQVYAQAFTIYALCAYHQLTGDAQALARALECFNLIEQHAIDSVNQ
                     GYLEAFTRDWGVIEDLRLSEKDLNYPKSQNTHLHILEAYTTLYQAQPSAEVRAALQYNI
                     EMFDRYMIDRNTHHLRMFMDLQWNDFSPGYTYGHDIEASWLIAKALESLGDEAYTRALT
                     PTLIRIAEVTLQEGIGELGQVVDSYDFASATVNNDTVWWVQAEALVGFLYAYSVSGEQK
                     YFAAAEAVWAFIKKYQIDNEAGEWLWLSRLHAPSATPYYKVGFWKCPYHNGRAMMEAIR
                     YLQGDASVHTT"
     gene            2542..3912
                     /gene="man5A"
     CDS             2542..3912
                     /gene="man5A"
                     /note="mannosidase; glicoside hydrolase family 5"
                     /codon_start=1
                     /transl_table=11
                     /product="GH5_7|GH5"
                     /protein_id="AAS19695.1"
                     /translation="MKNIIAVIVGFVSILLLAACDKKTPVAESNSAVAPTANVATSPAH
                     EHFVRVNGGHFELQGKPYVITGVNMWYAAYLGAPNEVGDRDRLAKELDNLKAIGVNNLR
                     VLAVSEKSEINSAVKPAVTNGFGNYDETLLQGLDYLLVELAKRDMTVVLYFNNFWQWSG
                     GMTQYMAWIEGEPVQDPNVTNEWEAFMAKSASFYRSEKAQQEYRKTLEKIITRVNSING
                     KAYVDDATIMSWQLANEPRPGNSQTTAEEKQIYIDWVHAAAAYIKTLDAHHLVSSGSEG
                     EMGSVNDMQVFIDAHATPDIDYLTYHMWIRNWSWFDKTKPAETWPSAWEKAQNYMRAHI
                     DVAKQLNKPLVLEEFGLDRDMGSYAMDSTTEYRDNYFRGVFELMLASLEQGEPSAGYNI
                     WAWNGYGRTTRANYWWQEGDDFMGDPPQEEQGMYGVFDTDTSTIAIMKEFNARFQPK"
     gene            4040..5257
                     /gene="aga27A"
     CDS             4040..5257
                     /gene="aga27A"
                     /note="alpha-galactosidase; glycoside hydrolase family 27"
                     /codon_start=1
                     /transl_table=11
                     /product="GH27"
                     /protein_id="AAS19696.1"
                     /translation="MIKKLLINAGLFGFALAASMSVSAQKFEHLAKTPQLGWNSWNTFA
                     CDVNEKMIREMADAMVASGMKDAGYEYINIDDCWHGERDKQGFIQVDKKSFPSGMKALA
                     DYVHSKGLKLGIYSDAGNTTCAGRPGSRGHEYQDAVTYASWGIDYVKYDWCDTKDINPK
                     AAYATMRDAIHKAGRPMLFSICEWGDNKPWEWATDVGHSWRTTGDIYPCWNCEHNHGSW
                     SSWGVLPILDKQAGLRKYAGPGHWNDMDMMEVGNGMNEDEDRAHFSLWAMMASPLIAGN
                     DLRKMSEATKKILTNKDMLAINQDKLGIQAMKWIDEGDIEIYVKPLEKGDYAVLFLNRA
                     DTTVNYSLDWGFHYMKDDISKHEIFFDKKKFNWRDIWNGGKGSTAEKLNLTMAAHSVAV
                     LRLTPQ"
ORIGIN
        1 cagcacaaaa aattttaact acgggtgtaa acgattacgt tataagttcg atgtgtgatg
       61 aaacactcat cagaatgaca cactggaaaa actcattgga gtatttgaaa catgagtagt
      121 tttaaagaaa aagcaaaagc cttactgcaa caacatgaaa cgctaatcac gaaaaaaaat
      181 gctgtaaaac gcgatggcaa cggtatttat gattgttacg aaaacccgat tctcaccgcc
      241 gaacatgctc cggttttttg gcgttacgat ttaaatgaaa aaaccaatcc gcatttaatg
      301 gagcgccagg gcattaatgc cgcgtttaat tccggtgcaa tgtattggaa cggtaaatac
      361 attttagcgg tgcgtgtaga aggtgtggat cgcaaatcct tttttgcgat tgcggaaagc
      421 ccaaatggta tcgacaactt ccgcttttgg gattacccca tcaccatgcc ggaaacagat
      481 cgccccgata ccaacgtgta cgacatgcgt ttaaccaatc acgaggatgg ttacatctac
      541 ggtttgtttt gtaccgagcg caaagattta aatcacccga acgatacctc tgcggcggaa
      601 gcgcaatgtg gtattgcacg caccaaagat ttaattacct gggagcgcct gcccgatttg
      661 gtgacctact ccggtcagca gcgcaatgtg gtattgcacc cggaatttat cgacggcaag
      721 tacggtttgt ttacccgccc acaagacggt tttatcagcg tgggcgccgg tggtggtatt
      781 ggttggggtc tggtggacga catgactcgc gccgaagtga aatcggaagt gattgtggat
      841 ggcaaggttt atcacaccat taaagaagtt aaaaatggcc aaggccctgc gccgattaaa
      901 actgccgaag gctggttgaa cctcgcccac ggtgtgcgta ataccgccgc cggtttacgt
      961 tatgtgctct acatgttcat gaccgaattg gagcgcccct gggtagtaac ccatcgccct
     1021 gccggacatt ttattgcacc gcacggtgct gaacgggtgg gtgatgtatc caatgtcgct
     1081 ttctcgaacg ggtggattgc gaacgaaaaa aatgaagtgt ttatttatta cgcctcatcg
     1141 gatacacgca tgcacgtggc aacgtctacc gtggaaaaac tgatcgacta ttgcaaaaac
     1201 accccggaag atggcctgcg ttctgcggca tcggttgcgg tgcgcaatga attaatcagt
     1261 gcgaatttgc aagtaatgaa ggatttggtt taaaccatga acgccgccat gcttccttca
     1321 tctgtcattg tgttgcaaca agaatttcgc gcagaactta gcgctattgc tgattggtgg
     1381 gccagccata ctatcgatca gcaacagggt ggtttttacg gtgaagtggg ggcagacaat
     1441 ctgcctgttc cctctgccag taaaggcatt attctgaatg cgcgtattct ttggtttttt
     1501 agtgaaaccg cgcagcaaat caataacccg gtttatcgcg attgtgctga gcgcgcatac
     1561 cattatttgt gtgcgaattt ttttgatgat gtgcacggcg gtgtgtattg ggagctggat
     1621 gcggcgggcg cgccgatcaa taccaaaaag caagtttatg cgcaggcatt tactatctac
     1681 gcgctctgcg cttaccacca actcacgggt gatgcacagg cacttgcccg tgcgctggaa
     1741 tgttttaatt taattgaaca gcatgctatc gactcggtaa atcaaggcta ccttgaagct
     1801 tttacccgtg actggggtgt gattgaggat ttacgtctca gcgaaaaaga tttgaattat
     1861 cccaaatcac aaaataccca tctgcatatt cttgaagctt acaccacctt gtatcaggcg
     1921 cagccgagtg cagaagttcg tgcagcactg caatacaata ttgaaatgtt tgatcgctac
     1981 atgatcgaca gaaacacgca tcacctgcgt atgtttatgg acctgcagtg gaacgatttt
     2041 tcacccggtt atacctacgg gcacgatatt gaagcgagct ggttaattgc caaagcattg
     2101 gaatcgctgg gtgacgaagc ctatacgcgc gcacttacac cgacattaat tcgtattgcc
     2161 gaagtgacgc tgcaagaagg cattggcgag ttggggcagg tggtggactc ttacgatttt
     2221 gccagtgcca ccgtgaataa cgacaccgtg tggtgggtac aagcagaagc gttagtgggt
     2281 tttctttatg cttacagtgt cagtggcgaa caaaaatatt ttgcggcggc ggaagcggtg
     2341 tgggccttta ttaaaaaata tcaaattgat aatgaagcgg gtgagtggtt gtggttgtcg
     2401 cggctgcacg cgcccagcgc cacaccctat tacaaagtgg gtttttggaa atgcccctac
     2461 cataatggcc gcgccatgat ggaagcgatc cgttatttac agggcgatgc ttctgtacac
     2521 acaacttaat ctggaaaaat aatgaagaat attattgctg ttattgtcgg atttgtatcc
     2581 atattgttgt tagctgcctg cgataaaaaa acacccgttg cagaaagtaa ctcagcagtt
     2641 gcacctacgg ccaatgttgc aacatcgcct gcccacgaac attttgtgcg cgtcaacggt
     2701 gggcactttg agctgcaggg taaaccctat gtgattaccg gcgtaaatat gtggtacgcg
     2761 gcttatcttg gtgcgcccaa cgaagtcggc gatcgcgatc gcttggcgaa agagctagat
     2821 aatttgaaag ccatcggcgt aaataatttg cgtgtattag ctgtatccga aaaaagtgaa
     2881 attaattcag cggtaaagcc ggccgtgacc aatggttttg gcaactacga tgaaaccttg
     2941 ctgcaaggtt tggattattt attagttgaa ctcgctaaac gcgatatgac cgtggtgctg
     3001 tattttaata atttctggca atggtcgggc ggtatgactc aatacatggc ctggattgaa
     3061 ggcgagccag tgcaagaccc gaacgtgact aacgaatggg aagcctttat ggccaagtcg
     3121 gcgagtttct accgcagcga aaaagcgcag caggaatatc gtaaaacgct ggaaaaaatt
     3181 attacccgcg tgaacagcat taacggcaaa gcctatgtcg atgacgcgac gattatgtcc
     3241 tggcaattgg ctaacgagcc gcgccccggt aattcacaaa cgacggcgga agaaaaacaa
     3301 atttatatcg attgggttca cgctgctgcg gcttatatta aaactttgga tgcacatcat
     3361 ttagtgagca gtggtagtga aggtgaaatg ggttcggtaa atgacatgca agtgtttatt
     3421 gatgcacatg ccacgccgga tatcgattac ctcacctacc acatgtggat tcgcaactgg
     3481 agctggtttg ataaaaccaa accggcagaa acctggccat cggcgtggga aaaagcacag
     3541 aactatatgc gtgcacacat tgatgttgcc aaacaattaa acaaaccctt ggtgttggaa
     3601 gagtttggtt tggatcgcga tatgggttcc tacgcgatgg attccaccac cgaatatcgc
     3661 gataactatt ttcgcggtgt atttgaattg atgctggcga gccttgaaca aggtgaaccg
     3721 agtgcgggtt acaacatttg ggcctggaat ggttatggcc gcaccacacg cgcgaactac
     3781 tggtggcaag agggcgacga ttttatgggt gacccgccac aagaagagca gggcatgtac
     3841 ggtgtgtttg ataccgatac atctactatt gccattatga aagagtttaa tgcgcgtttt
     3901 cagccgaaat aatttgcacg ctgcgtcagt aaaaaactgc aatgaaaatt gcagtttttt
     3961 ttgacgccgt attaaattat cagattatta atataatgat gtattcgacg atatcctcac
     4021 gacaaaaaag gtcaacacca tgataaaaaa actattgata aacgctggtc tgttcggatt
     4081 tgcattggct gccagcatga gtgtcagtgc gcaaaaattt gagcatctcg ccaaaacccc
     4141 gcagcttggt tggaatagtt ggaatacttt tgcttgcgat gtaaatgaaa aaatgattcg
     4201 cgaaatggct gacgctatgg tggcatcggg catgaaagac gcgggttatg aatatattaa
     4261 tatcgacgac tgctggcacg gcgagcgcga caaacaaggt tttatccagg tcgataaaaa
     4321 gagttttccg tcgggcatga aagcgctggc ggattatgtt cacagcaaag gattaaaact
     4381 cggcatctat tctgacgcgg gcaatactac ctgcgccggt cgcccaggca gtcgcggcca
     4441 cgaatatcag gatgcggtga cctatgcgag ttggggcatt gattacgtga aatacgattg
     4501 gtgcgatacc aaagacatca accccaaagc tgcttacgcc accatgcgcg atgccattca
     4561 taaagcgggc cgccctatgt tattcagcat ttgcgaatgg ggcgataaca aaccttggga
     4621 atgggcaact gacgttggtc attcatggcg caccacgggt gacatttacc cctgctggaa
     4681 ttgcgagcac aaccacggtt cctggtcgtc gtggggcgta ttacccattc tggataaaca
     4741 agccggtttg cgtaaatacg cgggccccgg ccattggaat gacatggaca tgatggaagt
     4801 cggcaacggc atgaatgaag acgaagaccg cgcacatttt tccctgtggg cgatgatggc
     4861 ctcaccgctg atcgccggta atgatttacg caagatgagc gaggccacca aaaaaattct
     4921 caccaacaag gacatgctcg ccattaatca ggacaaactg ggcattcagg cgatgaaatg
     4981 gattgatgaa ggcgatatcg aaatttatgt caaacccttg gagaaaggcg actacgccgt
     5041 actctttttg aatcgcgcgg ataccacagt gaattacagt ctggattggg gcttccacta
     5101 catgaaagac gatatcagca aacatgagat tttctttgat aagaaaaaat tcaactggcg
     5161 cgatatctgg aatggcggca agggctccac tgcagaaaaa ttaaacctga ccatggctgc
     5221 ccatagcgtg gcggtgttgc gtttaacacc gcagtaagtt tttgtgctgt atgtcattta
     5281 acaaagccgg cacttgccgg ctttgttgca ttttgcctgg ctcaaattcg ggtatatagt
     5341 gctgccttgc catatctgca gggagaaatc aggtgcagct gtttgcgtac aatcaagtgg
     5401 ggcgcccgga tgaattatta atctgggtcg gcatgttggc ggtttaccct ccgccgtcag
     5461 tgatttttga aattgccggt cagccggtgg ccgcacaatt attgccgccg ggttttgaag
     5521 cgctgggtga tgaagtctgc gatgcgcgcg gcaaagcgat taactatcgc gggattttta
     5581 aattggcgtg gccggatacc gctgagcgtt tcacggtcac ggttagcgct aatggctgtg
     5641 tgaccacact cagtagccgc cgaccaccac aacaattgcc gctaaaaaat gcggccagtt
     5701 ttaacctgct gctctcctcc tgttattacc aacccaatga taaaagtcgc gcattggcgg
     5761 atgtgataaa acacattaag cccgcgccgg atttcacggt gcttgcgggt gatcaggtgt
     5821 atctggatct gccctcgcaa caagatttac cctgcaacaa aacggcgttg gcaaaaacgc
     5881 tggggaaaaa atatcaactc aattggtttt ccaacagcgc gcaacagccg gggttggcca
     5941 atattttgcg ccaaggccca gtgctttgtg tgccggacga tcacgaattc tggaataact
     6001 ttccgctctt ccaagcccag ttggacaata cccactgcga acaggatcgt gccaattggg
     6061 ctgagttagc caatcgttta tatgagcgct accagcgttc cccggcgcaa gcagcgggtt
     6121 ttttccggca ggatattgcg ccgctaagca tgttgttttt ggatgggcgc acccagcgcg
     6181 atgacgacgg cgtgcagatg tttaatgccg caactcatgc ggcgattggg cagtggaaaa
     6241 gcgatttgct ccagcgcaaa caacaagggc acgccgcggt gggtttgctc agttcggggc
     6301 aggccctatt gattgaaacc cccggcctct gggcgtgcaa aattgccgat atggaaatgc
     6361 ccaactacgc cgattttgcg ctgatcacca cggcgctcgc cgaattattt gccgccgaaa
     6421 taccggtgat ttatatcacc ggtgatgtgc attgggggcg catagtcgag ggtaaaaatg
     6481 tgcgcggcaa tactctcttt tatgaagtga
//
