LOCUS       FP476056               19612 bp    DNA     linear   BCT 27-MAR-2015
DEFINITION  Zobellia galactanivorans strain DsiJT chromosome, complete genome.
ACCESSION   FP476056
VERSION     FP476056.1
DBLINK      BioProject: PRJEB8976
            BioSample: SAMEA3315864
KEYWORDS    .
SOURCE      Zobellia galactanivorans
  ORGANISM  Zobellia galactanivorans
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Zobellia.
REFERENCE   1
  AUTHORS   Thomas,F., Barbeyron,T., Tonon,T., Genicot,S., Czjzek,M. and
            Michel,G.
  TITLE     Characterization of the first alginolytic operons in a marine
            bacterium: from their emergence in marine Flavobacteriia to their
            independent transfers to marine Proteobacteria and human gut
            Bacteroides
  JOURNAL   Environ. Microbiol. 14 (9), 2379-2394 (2012)
   PUBMED   22513138
REFERENCE   2  (bases 1 to 19612)
  AUTHORS   Genoscope -,C.E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
FEATURES             Location/Qualifiers
     source          1..19612
                     /organism="Zobellia galactanivorans"
                     /mol_type="genomic DNA"
                     /strain="DsiJT"
                     /db_xref="taxon:63186"
     gene            complement(1..1485)
                     /locus_tag="ZOBELLIA_2194"
     CDS             complement(1..1485)
                     /locus_tag="ZOBELLIA_2194"
                     /EC_number="3.1.6.-"
                     /function="RO-SO3- + H2O = ROH + HSO4-"
                     /note="The family 1 of sulfatases is composed of enzymes
                     that require the posttranslational oxidation of a conserved
                     cysteine (or serine) to a catalytic formylglycine to
                     hydrolyze various sulfate ester substrates; Belongs to the
                     family 1 of sulfatases (S1: formylglycine-dependent
                     sulfatases), subfamily 20; Putative lipoprotein signal
                     peptide cleaved between the residues 27 and 28; Possibly
                     localized in the outer membrane; Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Sulfatase, family S1-20"
                     /protein_id="CAZ96350.1"
                     /db_xref="GOA:G0L5L4"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="InterPro:IPR024607"
                     /db_xref="UniProtKB/TrEMBL:G0L5L4"
                     /translation="MNTTMRLRFVPNLSILFVFSLLLSLGACKEETKTVTTTTANAQQK
                     PNIIYILADDLGYGDLSSYGQEKFETPRIDELAQRGMKFTQHYSGAPVCAPARSSLMTG
                     QHTGHTPIRGNMELEGEGQTPLPGNSVTIAEMLKKAGYITGAFGKWGLGFIGTEGDPVA
                     QGFDEFYGYNCQRMSHRYYPTHLWHNEEKVLLEGNDWTKTETYAPDKIQAATLNFIETN
                     KEKPFFAYVPFVLPHAELISPNDSIFDKFKDKYEETPYTAENLYTSDYGPDIVPQEYAP
                     QLKPHAAFASMVYRMDVYVGQILDKLDELGIADNTIVMFTSDNGPHAEGGADPEFFNSS
                     GGLKGIKRDLYEGGIRSPFLAVWPNKIKAGSTSDHVSAFWDLMPTLAEITNTDVPASSD
                     GISFLPTLLGKKDQKQHEYLYWEFSIQNGRKAVRKGDWKGVIYNFNKQPQGPFELYNLA
                     DDPEETHNLADQHPEIVTEMKQIMENASEESELFPF"
     gene            1773..4502
                     /locus_tag="ZOBELLIA_2195"
     CDS             1773..4502
                     /locus_tag="ZOBELLIA_2195"
                     /function="Receptor and transporter activity"
                     /note="Protein localized in the outer membrane involved in
                     uptake of macromolecules that are too large to diffuse via
                     the outer membrane porins channels or are encountered at
                     very low concentrations; Contains a carboxypeptidase
                     regulatory domain (29-112) and the Plug module (125-207)
                     acting as a channel gate; The signal peptide is cleaved
                     between the residues 26 and 27; Specificity unclear"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Receptor"
                     /protein_id="CAZ96351.1"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR014766"
                     /db_xref="InterPro:IPR027804"
                     /db_xref="UniProtKB/TrEMBL:G0LBP9"
                     /translation="MVKFQLKYGFILILFILSLTSMQSQSSGAISGRVLSAEKEALEAV
                     AVVLLGVKDSTYVDYTITDANGDFNISEVPKGNYILNISSLGYLSVYKDLSYTGEAIAL
                     GDLILREDTYELEGVTLTAVVPVQIKQDTIAFNANSFKVDSDDNLEELLNKIPGMEIDS
                     DGKVNAQGSEVTKIMIDGKEFFGGDPSIVLKNLSADAIANVEIIDQKSDESELTGVADG
                     NKEVVINFTLKESRKNNGFGKISAGGGLDNRYFGNLNYNKFTSKTQFSVIGKINNINIT
                     GSNIQDFLKNADGVDGDGDDEEEQNSAQRTKSLSGYLNTAVAGVNVGHEFKEKESLNGD
                     YFYNYSKNDGSSLSKRITFSNANNFDYEAENEYINTKNNHNLNLDYKNKSNPNNSLIVK
                     GQLISDEVNSYSNRKGRYFKEEGLSTKNDNLSTVDRDRKRGNMALNFYQKLRKKGRSLA
                     TGLKTTFNRSTMKNDQYTLITRQVNTQNESVKELNTLRDQKLNTSAINFNFKYTEPLGG
                     NHYLKLESYMGNKIDRENVYQNKTTITSSNKEEILQYDYDHNENNYQTRLAHSYNVGTI
                     NTYAAMELQDIVRSFGVVNEASMTTDRLFVNPIATVQYIPKRGRKYRLSYKRNVRTPRP
                     TESSTVVNDLNPFSIRKGNPDLVAEKIDALTVSAIVHDFRSSLSFNTRLQYQYTKDAII
                     KSVDVDEDYVRTLSYQNNGIKRRLNTSLGFSQKLKGLGLRYSIRNRNFFNTNNAIINFQ
                     LNEVTSRDFLVSATLENNNKSMVDLKAGATYSVNNTSFSIEHDLDRKYTKQQYFSMLDC
                     DLGEKVNFNTQFDYFIYTDNAFASNENIPLWNATVSYAFTKQKNHVLKLLFIDLLDKNI
                     DIYRRSTTNYFEETTSESLGRYFIVSYMHKLNNRPRKG"
     gene            complement(4537..6027)
                     /gene="sgsA3"
                     /locus_tag="ZOBELLIA_2196"
     CDS             complement(4537..6027)
                     /gene="sgsA3"
                     /locus_tag="ZOBELLIA_2196"
                     /EC_number="3.10.1.1"
                     /function="N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine
                     + sulfate"
                     /note="The N-sulfoglucosamine sulfohydrolase, called also
                     Sulfoglucosamine sulfamidase is involved in the
                     glycosaminoglycan degradation. It hydrolyzes the N-sulfate
                     groups from the D-glucosamine-N-sulfate-6-O-sulfate
                     residues in heparan sulfate and heparin; Belongs to the
                     family 1 of sulfatases (S1: formylglycine-dependent
                     sulfatases), subfamily 8; Signal peptide putatively cleaved
                     between the residues 25 and 26; Possibly localized in the
                     periplasmic space; High confidence in function and
                     specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="N-sulfoglucosamine sulfohydrolase, family S1-8"
                     /protein_id="CAZ96352.1"
                     /db_xref="GOA:G0L5L5"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="InterPro:IPR024607"
                     /db_xref="UniProtKB/TrEMBL:G0L5L5"
                     /translation="MMKKNTIKKTFAASIYLLFGCMLFAQEKPNIIWLMAEDMSLDLEC
                     YGMKGVKTPNLNKMAEEGVKFENCFVTNPICSPSRSAMLTGTHQVKINAHHHRSNRDVP
                     LDERFKPFTYWLRKAGYTTILGHHGVMGKGRKIDANFKHEAIGPWDGKTQFGLFDKYDT
                     FEKEDQPFFAQIQLVASHRGDWWDEVRENSKHPVDPADVELPKFMADDPVIRLDWAKYL
                     DQIEFLDHEVGMIFQELEDKGMADNTVVIFIGDNGRCNIRGKGYLQDPGLHIPLIVYYP
                     KDLKAGQVRRDVVSATDITATVLDFADVKIPDYMTGKPIFDKNFKREYVYGARDLWDEI
                     EEKSRAITSGEWKYIRNDMPEVPFDAHQAYLEFYRPAVHRMRALKAEGKLNESQKLFFA
                     PRKDKEELYDLKNDPQELHNLAEDPKYDKVLQKMRQKTLEFDRQMEPVSSVYHPVHAIS
                     VDALEWVKKERPEDYQRMLAGEEIGFKKAVEGYKASLK"
     gene            complement(6047..7387)
                     /locus_tag="ZOBELLIA_2197"
     CDS             complement(6047..7387)
                     /locus_tag="ZOBELLIA_2197"
                     /EC_number="3.1.6.-"
                     /function="RO-SO3- + H2O = ROH + HSO4-"
                     /note="The family 1 of sulfatases is composed of enzymes
                     that require the posttranslational oxidation of a conserved
                     cysteine (or serine) to a catalytic formylglycine to
                     hydrolyze various sulfate ester substrates; Belongs to the
                     family 1 of sulfatases (S1: formylglycine-dependent
                     sulfatases), subfamily 16; Signal peptide cleaved between
                     the residues 19 and 20; Localized in the periplasmic space;
                     Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Sulfatase, family S1-16"
                     /protein_id="CAZ96353.1"
                     /db_xref="GOA:G0LBQ0"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="InterPro:IPR024607"
                     /db_xref="UniProtKB/TrEMBL:G0LBQ0"
                     /translation="MKNTLFLAALFISIFKGIAQEQPNIVLLFVDDYGWADVGFRNPKF
                     HTPNIDQLKKDGLNFNRAYIATPTCSPSRASLLTGKEPVRFQMVRHIIDSKEKAAKNNV
                     PGGEFNLWPTDPVQMPSRNWLPLEEVTYAERLKEFGYYNMFIGKWHLGSKKYHPVHQGF
                     DAQYGTGDHGHPGNYYAPFFKKGNPLPDSPEGTYLTDVLTDGAEKFIGEYDKEQPFMLS
                     LWYYNVHGPHIGRKDWVERYKKEGLTGKDAEYAAMVSTMDESVGRVRKAIEAKGIADNT
                     VIILLSDQGGYFSNAPLSGGKTGGNTLGEGGARVPFIVHYPGVTTPGISCDVPVQSIDL
                     FPTLVEIASGKKPKTKDIQGKSLMPLLQGKKFKERNLFFFRSYEDQYTAIMNGDWKMVK
                     YHSGRHDLFNIRTDRSESKNLIDIEVDRAKKMKKQLEKWEKEAVPEF"
     gene            complement(7404..9530)
                     /locus_tag="ZOBELLIA_2198"
     CDS             complement(7404..9530)
                     /locus_tag="ZOBELLIA_2198"
                     /EC_number="3.1.6.-"
                     /function="RO-SO3- + H2O = ROH + HSO4-"
                     /note="Modular protein consisting of a N-terminal domain
                     belonging to the family 1 of sulfatases and a C-terminal
                     PA14 domain putatively involved in the protein or
                     carbohydrate binding; The family 1 of sulfatases is
                     composed of enzymes that require the posttranslational
                     oxidation of a conserved cysteine (or serine) to a
                     catalytic formylglycine to hydrolyze various sulfate ester
                     substrates; Belongs to the family 1 of sulfatases (S1:
                     formylglycine-dependent sulfatases), subfamily 20; Contains
                     a lipoprotein signal peptide cleaved between the residues
                     22 and 23; Localized in the outer membrane; Family
                     membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Sulfatase, family S1-20"
                     /protein_id="CAZ96354.1"
                     /db_xref="GOA:G0L5L6"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR011658"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="UniProtKB/TrEMBL:G0L5L6"
                     /translation="MSIQKFSVQLLSVLVFTATLLSCAEKKQQEKADQTAGPKKKPNII
                     YILTDDLGYGDIGVFFQNQRQKENDRSEPWALTPQLDKMASEGAMLTHHYTAAPVCAPS
                     RSSLLLGVSQGHANVRNGQFDKALADNHTLGNLMQRAGYTTAAIGKWGLQGSSRWSENG
                     DSWPAHPNNRGFDYYYGYMRHRDGHEHYPVEGIYRGKKEVYENKNEVSEGLDKCYTGDL
                     WTAVAKKWIVEQVKEKDAQEPFFMYLAFDTPHAVLELPTQAYPEGGGLNGGLQWIGQAG
                     NMINTASGEVDSYIHPDYAHATYDHDKNPETPEVAWPDVYKRYATSTRRIDNEVGDLLQ
                     LLKDLNIDENTLVIFNSDNGPSIESYLPEEEYAADFFNSFGPFNGIKRDVLEGGERTPL
                     IARWPNKIPAGQVIDAPSISYDWLPTFSEAAGYTPPANTDGISLLPILTGTGEQEESLI
                     YVEYNQGGKTPEYEEFAPNNRGRRRKQMQMMRMGDIVGLRYDIQSADDDFELYDVTKDS
                     HQTENLAGKPDMTALQSRMKERALQVRMPDTAAARPYDPAMVSPLPVPKVSKGLKWAAF
                     EDSAPWLPKVDGLSPIANGTSQTPTLENIVGKGEVFLFEGFIKIPTDGNYTFSLATSGK
                     AFVRIHEASVIDADYGYEAGTLKEGSMNLKAGYHPIRIYYKKKTGTKPTLDIQWSGPTI
                     EKGPIPESAFYSNN"
     gene            complement(9562..11454)
                     /locus_tag="ZOBELLIA_2199"
     CDS             complement(9562..11454)
                     /locus_tag="ZOBELLIA_2199"
                     /EC_number="3.2.1.22"
                     /function="Alpha-galactosidase"
                     /note="Alpha-galactosidase catalyzes the hydrolysis of
                     terminal, non-reducing alpha-D-galactose residues in
                     alpha-D-galactosides. Belongs to the family 110 of the
                     glycoside hydrolases. Likely adopts a right handed parallel
                     beta-helix fold. Signal peptide cleaved between the
                     residues 26 and 27. Localized in the periplasmic space.;
                     High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="GH110"
                     /protein_id="CAZ96355.1"
                     /db_xref="GOA:G0L5L7"
                     /db_xref="InterPro:IPR006626"
                     /db_xref="InterPro:IPR011050"
                     /db_xref="InterPro:IPR012334"
                     /db_xref="UniProtKB/TrEMBL:G0L5L7"
                     /translation="MKNTSPYKNLIALTLCLVGIMGFSQSASLNEKVLFKTDIADDATP
                     ALLARIRQAKDHPISQIKFEKGTYHFYPDKAFERFTRISNHDDVMAKTALPLLGFKDLV
                     IDGQGSTFIYHGRMIPFLIEGSENITVQNVTIDWAMPFHSEGTIVANDEKNNTFDIHFS
                     DEYPYEIRNEQLIFLKEYYEHDLGQSILYDPIRNAIAFDTEAYTPITTIRNSKTQRNFD
                     KIKYKYKVDPRSKVEKVRGKEDNLRVKEIKPGTVRIYNHRKKLPPVGMILVCKGDQSLN
                     RLAPAFRISGTDGFNANNVTVNHAGGMGLIAENSSDLILDGFHVKPSHGRMVSTSADAT
                     HFVGCRGKVVLKNCTFNNQLDDATNVHGTYQEVVDILSENSIGVRMGHAQQQGFPIGTP
                     NDTIGLVRLSDSFFAYDHLTLKAIENINGRYQIVTFNEKLPATLKVGDLIENLEGYPDL
                     LVQNCNISRNRARGLLISNPKNTLIENNFFSTEMEAILVPVESSHWFESGNAANLVIRN
                     NTFQDNQHSGFNRGVIRFVTDDDNDNIAFKNIEITNNTFNQFDNLILEISNVDGLLFEG
                     NKITNSGTFPMLHPENPAIKVKASKNIVFKKNGYKGKAKPILEKDESQPKLKFK"
     gene            complement(11451..13256)
                     /locus_tag="ZOBELLIA_2200"
     CDS             complement(11451..13256)
                     /locus_tag="ZOBELLIA_2200"
                     /EC_number="3.2.1.22"
                     /function="Alpha-galactosidase"
                     /note="Alpha-galactosidase catalyzes the hydrolysis of
                     terminal, non-reducing alpha-D-galactose residues in
                     alpha-D-galactosides. Belongs to the family 110 of the
                     glycoside hydrolases. Likely adopts a right handed parallel
                     beta-helix fold. Signal peptide cleaved between the
                     residues 19 and 20. Localized in the periplasmic space.;
                     High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="GH110"
                     /protein_id="CAZ96356.1"
                     /db_xref="GOA:G0LBQ1"
                     /db_xref="InterPro:IPR006626"
                     /db_xref="InterPro:IPR011050"
                     /db_xref="InterPro:IPR012334"
                     /db_xref="UniProtKB/TrEMBL:G0LBQ1"
                     /translation="MKSTYVFVIFLLGVFHLTAQQTIQFQPDAEDMTPVVRKALENVSD
                     KNLTLVFETGVYKFLPDYATQRYSYITNHGNGLKNIIFLLEDFDSVTIEGNGAEFIFHG
                     QVAPFQFKNCKKVTVNDLSIDWDIPFTFQGEVVAVNEKEGWRDIKPFTEGYSWKLNNGR
                     ILFPDIDGFVFPEMGSTLAFDPEHKRVAHGAWDMSSRPRWVEKRPNGTLRFYEHLKQYP
                     SVGSILHSKGDHDHNRYAPAFQVTSSQNILLKNIKVHHALGMGFLFERTENITIDNCGV
                     YVREGSDRVVSTIADATHFANCKGDILIENCTFKHMLDDGTNVHGTYVVVDRIIDQRTV
                     RVALQHFEQMGFEFAAPNDEIWFIHQPNPKRGSVHTVASVSVVNDKYIDLSFNDGLPPE
                     LAVGDILENKTWNPTFTMRGCTVKDHRARNIVLKTPLKTVIENNNFSSMMSSVFFRGES
                     FFWFESGAVQDVLIQNNNFEYCAYSGMEHAVLYITPRLGKGFDQNIRYDRNIRFVNNRI
                     QTFGNRIVWADRVDGLEVSGNTIIQTKDAPELYPNAPMFEITKGKATKIIKNSYSGTNE
                     NIIKSDPTSTKTLQTKNNKGFKIKK"
     gene            complement(13284..14783)
                     /gene="sgsA4"
                     /locus_tag="ZOBELLIA_2201"
     CDS             complement(13284..14783)
                     /gene="sgsA4"
                     /locus_tag="ZOBELLIA_2201"
                     /EC_number="3.10.1.1"
                     /function="N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine
                     + sulfate"
                     /note="The N-sulfoglucosamine sulfohydrolase, called also
                     Sulfoglucosamine sulfamidase is involved in the
                     glycosaminoglycan degradation. It hydrolyzes the N-sulfate
                     groups from the D-glucosamine-N-sulfate-6-O-sulfate
                     residues in heparan sulfate and heparin; Belongs to the
                     family 1 of sulfatases (S1: formylglycine-dependent
                     sulfatases), subfamily 8; Lipoprotein with a signal peptide
                     cleaved between the residues 26 and 27; Localized in the
                     outer membrane; High confidence in function and
                     specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="N-sulfoglucosamine sulfohydrolase, family S1-8"
                     /protein_id="CAZ96357.1"
                     /db_xref="GOA:G0L5L8"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="InterPro:IPR024607"
                     /db_xref="UniProtKB/TrEMBL:G0L5L8"
                     /translation="MFQNQRHTMTSKLFTPVLIFLLLFVGCTTKDKTKDLAKKDAKNTS
                     PNIILFVSDDHGTDAIGAYGNKVIKTPNLDQLAAEGVRFNNAYCTSASCAASRSVILTG
                     LYGHATGSYGHVHDYHHFSSYDNVKSLPVLLEKAGYETARIGKYHVAPESVYHFQKVLE
                     ADPRNTVEMAEKCKDVLNSEKPFFLYFCTDDPHRGAPFQSDPWYLPNSFGNKKEGYEGV
                     ETVTYDPKDVVVPDFLPDSKETREEIAQYYQSVSRIDQGFGKLMKMLKDTGKDKNTVVI
                     YISDNGMAFPGAKTTVYEPGIKLPCIIKGPLAGLKGGSVNNALISWTDLTPTILDMANV
                     SYDSNAFHGKSFKKILAEENPKGWDEIYASHTFHEITMYYPMRVVRDKNFKLIRNIAWR
                     QEYPFASDLWAASTWQEVYRSEKEYFGKRKVKDYLFRPEFELFDLSKDPSESHNLADDT
                     NYKDVLEALKVKMKAFQKETKDPWLITWDHDNSLQGTGVNL"
     gene            complement(14938..16476)
                     /locus_tag="ZOBELLIA_2202"
     CDS             complement(14938..16476)
                     /locus_tag="ZOBELLIA_2202"
                     /function="Transporter activity"
                     /note="Protein probably involved in nutrient binding and
                     belonging to the SusD/RagB family; Gene very often
                     associated with a gene encoding for a TonB-dependent
                     receptor or transducer; Contains a lipoprotein signal
                     peptide cleaved between the residues 22 and 23; Probably
                     localized in the outer membrane; Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|C6Y217|8.A.46.1.3"
                     /protein_id="CAZ96358.1"
                     /db_xref="InterPro:IPR011990"
                     /db_xref="InterPro:IPR012944"
                     /db_xref="UniProtKB/TrEMBL:G0LBQ2"
                     /translation="MKRIYNTYTVLFAFIILLGFGSCEKLDEGSDGVVASSTFYSDENT
                     LDAGIIGIYGTLRRNTWGLDNYAHFTGADDLTSRLGSNKWLVLESDQFARTAGNSWATN
                     DYNGNYQTILACNSYIADAHPEGVEESVINAAHANAYFIRGLCYFRLATSFGDVPMPLD
                     PVPDLEIPLTPMREVMAQAIKDFEFAIKWAVNERDSNPTISNGRVSKTAAKAFLAKTYM
                     QLTGYPYNEADKWEQVKQLTGEIIADGVYSLTDDFAYNFQDPHQINKEVIFAHIMSKES
                     WPIITQNRVYGYRWANWMDLYMEWTYFNNFPEGYRKEFCTATEGNQFFEEFKNPIVTKY
                     TWGTPAPNSTDPLDLVVEHTWETSNDRIAMRYAEVLLMHAEAAANTGDLATALEELNRV
                     KRRAYAKGATKQAEVAALEPGFWMTPDPAIDYTLAELSTPQLMIDAIVNERAYEFLGEI
                     SGNRWLDLVRLEKVAEANATRDAREVPLIGDPADKNLWFTPIPATETTLNPNLN"
     gene            complement(16493..19612)
                     /locus_tag="ZOBELLIA_2203"
     CDS             complement(16493..19612)
                     /locus_tag="ZOBELLIA_2203"
                     /function="Receptor and transporter activity"
                     /note="Protein localized in the outer membrane involved in
                     uptake of macromolecules (here, the sulphated sugars) that
                     are too large to diffuse via the outer membrane porins
                     channels or are encountered at very low concentrations;
                     Contains a carboxypeptidase regulatory domain (35-109) and
                     the Plug module (125-232) acting as a channel gate; The
                     signal peptide is cleaved between the residues 32 and 33;
                     Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="CAZ96359.1"
                     /db_xref="GOA:G0L5L9"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /db_xref="InterPro:IPR014766"
                     /db_xref="InterPro:IPR023996"
                     /db_xref="InterPro:IPR023997"
                     /db_xref="InterPro:IPR027804"
                     /db_xref="UniProtKB/TrEMBL:G0L5L9"
                     /translation="MKRDQLFINWKHTLRISVSFLMLFLITSTIYAQDTTVSGTVVDQT
                     GLPLPGANILEQGTSNGVTADFDGNFSITVKSPNAVLTVSYIGFSTQQIATQGQKTLKI
                     VMQENAAGLDEVVVVGYGTQKKSDLTGSVGSISAKDLLKAPITKIGAGLQGKISGVNIQ
                     QTTGAPGQKMKIRIRGGSSINYGNEPLYVIDGFIGADISTINPNDIATINFLKDASATA
                     VYGSRGANGVVLITTVAPKEGPLKVTFDSNISMSSMVNKYEILPIDEMAELMNEQQASL
                     NKSPYFSAEDIAGFRSKGGTDWVDLITRQGYKQNYSLNLTGGSENLKYFFSANHLNDEG
                     VIKNSFYRRTSIRSNISGNIGERIQFKFNTYGTHIESQANGRGTGGTANPIGNAVIFPQ
                     MWESRDANGEFIDGTTYNGYNGAFLVGRLINPEMQVRQNQETLNDKITSNIDLNIDVAN
                     HLTLSLSAAGSLSMNYHGQRTLPDFINISRDNITAQQAYGRNNSWLANSILTYENNFGD
                     HNLKISGVYEFSKSINRNNTATVGNLSTLANEWHLLANGSPSSLNSGYGTGKLRSYMGR
                     LNYSFKDRYLLTASMRADGSSRFNADNRWGYFPSAAFAWRASEENFLKTSDFVSNLKFR
                     LGFGSVGNQAVGYGTIQQTFAASDSGDGRNYGWYQLDNGESVQGVVPGSITDKDLKWET
                     TTSYNAGIDFGILDNRLTGTIDVYSKKATDVIIAKSIPRYTGQSSITDNFADIDNNGIE
                     VGLNWNIVSKDDFQWSAYANFSKNKNIVQNLGQSGGESVEEIFIANEEPIGIWSLVGGN
                     NKFIVRKGESMGSFYGLKAIGIWQEDEAVEADSYSSRPGEVRYEDIDNDGAISAEDRQI
                     IGNASPDFTYGLGTSIAYKGLDLSIQCIGTVGNDIYNWTDNRLNYGILSTDYRNRWSPT
                     NTSSTQQVMPYGTDYSLTYVVSQYIEKGSFFKISNMTLGYNIPDNTTDALGLSSIRLYV
                     SSDNILTITNYSGLDPEGSSTALSSDSQSGVDAFSYPLTRTISLGLKVSF"
ORIGIN
        1 ttagaagggg aagagttccg attcttcgct ggcattttcc ataatctgct tcatttccgt
       61 aactatttcg gggtgttggt cggccagatt atgggtttcc tcggggtcat cggccaggtt
      121 atagagttcg aatgggcctt gcggttgttt gttgaaattg tagattactc ctttccaatc
      181 ccccttgcga acggctttac ggccgttttg aatggaaaac tcccaataga ggtactcgtg
      241 ctgcttttgg tctttttttc cgagtagggt gggaaggaat gaaataccat cactacttgc
      301 cggcacatcg gtattggtga tttccgcgag tgttggcata aggtcccaaa atgcggagac
      361 gtggtctgag gtggatccgg cttttatttt attgggccaa accgctagaa agggtgaacg
      421 gatacctcct tcataaaggt cccttttgat tccttttaag ccaccgctac tgttaaagaa
      481 ttctgggtct gcacctcctt cggcgtgggg accgttgtca ctggtgaaca ttacgatggt
      541 gttgtcggca ataccgagct catctaattt atccaatatc tgtcctacat aaacatccat
      601 acggtatacc atggaggcaa aggcggcatg tggcttgagt tgtggggcat actcttgggg
      661 aacgatatcc ggcccgtagt cggaggtata taaattctcc gctgtatacg gggtttcttc
      721 gtatttgtcc ttgaacttgt caaatatgga atcattcggt gaaattaatt ccgcatgtgg
      781 cagaacgaag ggcacatagg caaaaaaggg tttttctttg ttggtctcga taaagtttaa
      841 ggtagcggcc tgaattttgt cgggagcata ggtctctgtc ttggtccaat cgttcccttc
      901 gagcaaaacc ttttcttcgt tgtgccataa gtgggtcggg taatacctgt gggacatacg
      961 ttggcaattg tagccgtaga attcgtcaaa accttgggct acggggtcgc cttccgtacc
     1021 gatgaatccc aatccccatt ttccgaaagc tcctgtaata tagcctgctt ttttcaacat
     1081 ctcggcaatg gtcaccgaat tgcccggtag gggcgtttgc ccctcgcctt ccaactccat
     1141 gttgcccctt atgggggtgt ggccggtatg ttgtccggtc atgagggaag aacgggccgg
     1201 ggcgcaaacg ggcgcaccgc tgtaatgttg ggtaaatttc atcccccttt gtgccaattc
     1261 gtctatacgg ggcgtttcaa atttttcctg gccgtacgaa cttaagtcgc cataaccgag
     1321 gtcatcggct agaatataga tgatattcgg tttttgttgg gcgtttgccg tggttgtagt
     1381 tacggttttg gtttcttctt tgcacgcacc taagcttagt aaaagggaaa aaacaaaaag
     1441 aatggatagg ttggggacaa aacgtaatct catagtggta ttcatttagg ttttaaaagg
     1501 gtacgatcaa ttttttaggg tcgacaacta ttgggtatag ctatacgatt cgtttcatat
     1561 gttaaaggaa aaagataccg agcaatgtag ctattttacc tcgtataggt tagtcccgtg
     1621 atcatatgtt gcataattat agcaaattaa tcataagttc tgtggatttt gtactccaca
     1681 atacatttct tgtattctta tgtacaggtg ttcagttctt tcaaattatc tttagccttg
     1741 ttcatttcga attataattc aataaaagtt tgatggtcaa gtttcaatta aagtatggtt
     1801 ttatactcat tcttttcatc ctatcgctta cttcaatgca atcacaatct tccggtgcta
     1861 tctcgggaag ggtgctttcg gcggaaaaag aagccttgga agccgttgcc gtggtattgc
     1921 tcggggtaaa ggattccact tatgtggatt ataccattac cgatgcgaat ggggatttca
     1981 acataagcga ggtgcccaag gggaattata tccttaatat ttcgtccttg ggataccttt
     2041 cggtgtataa ggatctgtcg tacacggggg aagccattgc tttgggagac ctcattttaa
     2101 gggaagacac ttatgagctc gaaggggtga ccttgaccgc cgtagtgccg gtgcaaatca
     2161 aacaagatac catagccttt aatgccaatt cgttcaaggt agattccgat gataacttag
     2221 aggaattgtt gaataaaatt ccaggtatgg aaatcgattc cgacggaaag gtcaatgccc
     2281 aaggcagcga ggtgaccaaa attatgatcg atgggaagga attcttcgga ggggatcctt
     2341 ccatcgtact aaagaacctg tccgcagatg ccattgccaa tgttgagatc atcgatcaga
     2401 agagcgacga atcggaactc acgggcgtgg cggacggaaa caaggaggtg gtcatcaatt
     2461 ttaccttgaa agagtcgcga aaaaataacg gcttcggaaa gatctctgcc ggaggcggat
     2521 tggataatcg gtattttggg aatttgaatt acaacaaatt tacctctaaa acccagtttt
     2581 cggttattgg taaaatcaat aacatcaata taaccgggtc gaatatccaa gattttttaa
     2641 agaatgccga cggtgttgat ggcgatggcg acgatgaaga agagcagaac tcggcgcaaa
     2701 ggaccaaaag cctcagcgga tatctgaata cggccgtggc cggggtgaac gtggggcacg
     2761 agttcaagga aaaggaatcg ttgaacggcg attatttcta taactattct aagaatgacg
     2821 gtagctccct ttctaagcgt atcacttttt cgaatgccaa taactttgat tatgaggccg
     2881 aaaacgaata catcaacact aagaacaacc acaacctgaa cctcgattat aaaaacaagt
     2941 cgaaccctaa caacagtctt atcgtaaaag gccagttgat ctcagatgag gtcaatagct
     3001 attccaaccg taaggggagg tatttcaagg aggaaggact aagcactaaa aatgacaatc
     3061 tatctaccgt tgatagagat agaaaaagag gcaatatggc ccttaatttc tatcaaaaac
     3121 tgcgtaaaaa ggggaggagc ctagcgactg gtctgaaaac tacctttaat cggtctacca
     3181 tgaagaacga tcaatatact ttgatcacaa ggcaggtcaa tacccaaaat gaatcggtga
     3241 aggagctgaa taccctacgg gatcagaaac tgaatacttc ggcaatcaat ttcaatttta
     3301 aatataccga gccccttggg ggaaaccatt atttgaagtt ggagtcgtat atggggaata
     3361 aaatcgatag ggaaaacgtc tatcagaata agacgaccat tacttccagt aacaaagaag
     3421 aaatcttaca gtacgactac gaccataacg aaaacaatta tcagacccgc ttggcacata
     3481 gttataacgt ggggaccatc aatacctatg cggccatgga actgcaagac atcgtacgtt
     3541 cttttggcgt ggtcaatgaa gcgtcgatga cgacggaccg cttgttcgtc aacccgatag
     3601 caacggttca atatatccca aaaaggggaa ggaagtatag attgagctat aaaaggaacg
     3661 ttcgtacgcc acgacctaca gaaagctcga cggtggtcaa cgacctaaat cctttttcca
     3721 ttagaaaggg aaaccccgac ctggttgccg aaaagataga tgcccttacc gtatcggcca
     3781 ttgttcatga ctttaggtcg tctttgagtt ttaatacacg gttacagtac caatacacca
     3841 aagatgccat tataaaaagt gttgatgtgg atgaagacta cgttagaacc ctcagctacc
     3901 aaaacaacgg catcaaacgt cggcttaata cttccttggg ctttagccaa aaactaaaag
     3961 gtctgggact acgatattcc atccgtaacc gtaacttttt caatacgaac aatgcgatta
     4021 tcaattttca gcttaatgag gtgacttccc gagattttct ggtaagtgca accctagaga
     4081 acaataacaa aagtatggtg gaccttaagg cgggggctac ctatagcgtt aacaacacct
     4141 ctttttccat agaacacgat ttggaccgga agtataccaa gcagcagtat ttttcaatgc
     4201 tcgattgcga tttgggcgaa aaggtgaatt tcaataccca attcgattat tttatttata
     4261 ccgataatgc ctttgcctca aacgaaaata tccccctgtg gaatgccacg gtgtcgtatg
     4321 cctttacaaa acagaaaaac catgttttaa aactgttgtt cattgacctg ctcgataaaa
     4381 atatagatat ctacaggcgg agtacgacca actactttga ggaaacgaca tctgaaagtt
     4441 tgggccgtta ctttatagtg agttatatgc acaagttgaa caaccggcca cgaaagggat
     4501 aaaggaacaa aaatgaattc agtatgctct agattcctac ttaagactgg ctttatagcc
     4561 ttctacagct tttttaaagc ctatttcttc accggctaac attctttggt agtcttcagg
     4621 cctttccttt ttcacccatt cgagagcatc tacggaaatg gcgtgtaccg gatggtaaac
     4681 cgagcttacg ggttccattt gccggtcaaa ctccaaggtc ttttggcgca tcttttgaag
     4741 caccttgtca tatttggggt cttcggccaa gttgtgcaat tcctgggggt cgttttttag
     4801 gtcgtacaac tcttctttgt ccttgcgagg cgcaaagaac aacttttggc tttcgttcag
     4861 tttgccttcg gcctttaggg cacgcattcg gtgtaccgca gggcggtaga attccaaata
     4921 ggcctgatgg gcgtcgaagg gtacttcggg catgtcgttg cggatgtatt tccattcgcc
     4981 cgaggtaatg gcacgcgatt tctcttctat ttcatcccat aggtcccggg ctccatagac
     5041 gtattcgcgt ttgaagtttt tatcgaatat gggtttaccg gtcatgtagt cgggaatttt
     5101 tacatcggca aaatccaata cggttgcggt gatatccgta gccgaaacca catcccgtct
     5161 cacctggccc gcttttaggt ctttggggta atacacgatt aggggaatgt gcaaccccgg
     5221 gtcttgcagg tatcctttgc cccgtatatt acaacggccg ttgtcaccga taaaaatgac
     5281 cacggtattg tcggccattc ctttgtcttc cagttcttga aagatcatgc cgacttcatg
     5341 gtccaaaaac tcgatttggt ctaggtattt ggcccaatcc aaacgaatga cggggtcgtc
     5401 tgccataaac ttgggcagct cgacgtcggc tgggtctacg gggtgttttg agttttcgcg
     5461 gacttcgtcc caccaatcgc ccctatggct ggccacaagt tggatttgtg caaagaaggg
     5521 ttggtcttcc ttttcaaagg tgtcgtattt gtcgaagagg ccgaactgtg tttttccgtc
     5581 ccaaggaccg atggcctcgt gcttgaaatt ggcatctatc ttacggcctt tgcccattac
     5641 cccgtgatga cccaaaatag tggtatagcc agcttttctt agccaatagg tgaaaggttt
     5701 gaaccgttcg tctaagggta cgtccctgtt gcttctatgg tgatgggcgt tgatttttac
     5761 ctgatgcgtg ccggtcagca tggccgaacg actgggcgaa cagatggggt tggtcacaaa
     5821 gcaattttca aatttgacgc cttcctccgc cattttgtta aggttggggg tctttacgcc
     5881 tttcataccg tagcattcaa ggtcaaggct catatcttcg gccatgagcc agatgatatt
     5941 gggcttttcc tgggcgaaga gcatacagcc gaacaaaagg tatatactgg ctgcaaacgt
     6001 cttttttatc gtgttttttt tcatcatcgg tagtcttttt tagaggtcaa aattcaggta
     6061 ccgcctcttt ttcccatttt tcgagttgct tcttcatctt cttggcccga tctacttcta
     6121 tatcgataag gtttttcgat tcgctcctat ccgttctaat attgaatagg tcgtgtcggc
     6181 cactgtggta tttgaccatc ttccagtcgc cgttcataat ggcggtatat tggtcttcat
     6241 aacttcggaa aaagaacagg ttgcgttcct tgaatttctt accttgaagc aggggcatca
     6301 ggcttttgcc ttgaatatcc tttgtctttg gttttttgcc ggaagcgatc tctaccaaag
     6361 tcgggaaaag gtcaatggat tgcaccggga catcacatga aatacctggt gtggtcaccc
     6421 cgggatagtg aacgataaac ggaaccctgg ccccaccttc accgagggta ttgcctccgg
     6481 tttttcctcc cgataaggga gcattgctaa aataaccgcc ttgatccgat agcaatatga
     6541 taacggtatt gtcggcgata cctttcgctt cgatggcctt tctgaccctg cctaccgatt
     6601 cgtccatagt actgaccatg gcggcatact cggcgtcctt accggttaag ccttcctttt
     6661 tatagcgttc cacccagtct tttcggccta tatgtggccc gtgcacgtta tagtaccaaa
     6721 gcgatagcat aaagggctgt tctttatcgt attcgccaat gaatttttcc gcaccatcgg
     6781 tcaacacatc ggtcaggtag gtgccttcgg gggaatcggg tagcggattt ccttttttaa
     6841 agaagggtgc ataataattt ccagggtggc cgtggtctcc ggtgccgtat tgcgcatcga
     6901 aaccttgatg aacgggatgg tactttttgc ttcccaaatg ccatttccca ataaacatat
     6961 tgtaatagcc gaattcttta agtcgctcgg cataggtaac ttcctctaag ggcaaccaat
     7021 ttctggaggg catttgtacc ggatcggtag gccataggtt gaattccccc ccgggtacgt
     7081 tgttcttggc ggctttttct ttactgtcta tgatgtggcg taccatttga aaccgcacgg
     7141 gttccttgcc cgttaagagc gaagccctgc ttggactaca ggtaggtgta gcgatgtagg
     7201 cccggttaaa gttcaagcca tcttttttga gctggtcgat gttcggcgta tgaaatttcg
     7261 ggtttctaaa tccaacatcg gcccagccgt aatcgtctac aaagagcaat acgatgttcg
     7321 gttgttcttg ggctatacct ttaaaaatgc tgataaagag tgccgctaaa aaaagggtat
     7381 ttttcatttg actagagtgt tttttaattg ttggagtaaa atgccgactc aggaatcggg
     7441 cccttttcta tagtaggtcc gctccactgt atgtctaatg taggctttgt tcctgttttc
     7501 tttttgtagt aaattcgaat aggatggtaa ccggctttta aattcatgct accttctttt
     7561 aaggtgcctg cctcgtagcc gtaatcggca tcgatcacgc ttgcttcgtg aatgcgtaca
     7621 aaggccttgc cggaagtggc caaggaaaag gtgtaattgc cgtccgtagg tattttaata
     7681 aagccttcaa atagaaagac ctcgcctttt cctacgatgt tttccaatgt aggggtctga
     7741 gacgtgccgt ttgctatagg gcttagacca tcgactttag gtagccaggg tgcactgtct
     7801 tcgaatgcgg cccactttag gcccttgctt acctttggta cgggaagggg tgaaaccatg
     7861 gccgggtcat agggccttgc ggcggcggta tcgggcatac gtacttgtaa cgcgcgttct
     7921 ttcatccgtg attgtagggc cgtcatgtcg ggtttgcccg ctaggttttc agtttggtgg
     7981 gagtcttttg tgacatcgta gagttcaaag tcatcatcgg cactttggat gtcgtaccgt
     8041 aaaccgacga tatcgcccat gcgcatcatt tgcatctgtt ttcgtcgcct tccccggtta
     8101 ttgggtgcga attcttcgta ttcaggagtc tttccgcctt gattgtactc tacatagata
     8161 aggctttcct cttgttcgcc cgttccggtc aggatgggta ataaggagat cccatcggta
     8221 ttcgccggtg gtgtatagcc tgcggcttcc gaaaaagtag gaagccagtc ataggagata
     8281 cttggggcat cgatcacttg gccggcggga atcttgttgg gccatcgggc tattaacggg
     8341 gtgcgttcgc ctccttcgag cacatctctt ttaataccgt tgaaggggcc gaaactattg
     8401 aagaagtcgg cggcatattc ttcctcgggt aaatacgact ctattgatgg gccattatct
     8461 gagttgaaaa tgaccaaggt gttttcatca atgttcaggt cttttaggag ttgcagcagg
     8521 tctcctactt cattatcgat tctacgggtg ctggttgcat agcgtttgta tacatcgggc
     8581 caagcgactt cgggcgtttc tgggttttta tcgtggtcat aggtagcatg ggcataatcg
     8641 ggatgaatgt aggagtcgac ttcccctgat gcggtgttga tcatgtttcc ggcttggccg
     8701 atccattgta aacctccgtt caaaccacct ccttctgggt aagcttgtgt aggtagttcg
     8761 aggacggcat ggggcgtgtc aaacgccaaa tacataaaga aaggctcttg tgcgtctttt
     8821 tctttcactt gctctacgat ccattttttg gctacggccg tccataaatc gcccgtatag
     8881 cacttgtcga gtccttcgct tacctcattc ttgttttcgt aaacctcttt tttaccgcgg
     8941 taaatacctt caacgggata gtgctcatgg ccatccctgt ggcgcatata gccgtaatag
     9001 tagtcaaagc ccctattgtt ggggtgtgcc ggccacgaat cgccattttc cgaccacctg
     9061 ctggagcctt ggagtcccca tttgcctata gcggcggtgg tatagcccgc ccgttgcata
     9121 aggttgccca aggtgtggtt atcggccagg gccttgtcaa attggccatt acggacattg
     9181 gcatggcctt ggctgacccc caataataag gaggaacgcg aaggggcaca taccggtgcc
     9241 gccgtataat gatgggtgag catggcacct tctgaagcca ttttgtccaa ctgtggggtc
     9301 agggcccagg gttcgctacg gtcgttttct ttttgtctct ggttctggaa aaatacgcca
     9361 atgtctccat agccgaggtc atcggtaaga atgtagataa tattcggttt cttttttggt
     9421 ccggccgttt ggtccgcttt ttcttgttgc ttcttttcgg cacatgagag tagggtagct
     9481 gtaaaaacca gaaccgataa gagttgaact gaaaattttt gtattgacat gctttgctct
     9541 taatcttatt gtagtgttgt tttatttgaa cttcaactta ggctggcttt cgtctttttc
     9601 taagataggc ttggccttgc ccttataacc gtttttcttg aacacgatgt ttttggaggc
     9661 tttcaccttt atcgccggat tctcgggatg tagcataggg aaggttcccg aattggtgat
     9721 tttattccct tcgaacaata atccgtctac atttgaaatc tcaaggatca gattgtcgaa
     9781 ttggttaaag gtgttgttcg tgatctcaat atttttaaag gcaatgttat cattgtcatc
     9841 atcggttacg aaacgtatga cccctctatt gaacccactg tgttggttgt cttgaaaggt
     9901 attgttcctg atgaccaaat tagcggcatt gcccgactcg aaccaatgac tgctctctac
     9961 aggtactaag atagcttcca tttcggtact gaaaaagtta ttttctatca aggtgttctt
    10021 agggttggaa atcaataatc cacgtgccct gttacggcta atgttgcaat tttggaccaa
    10081 taggtcgggg tagccttcca aattttcgat taggtcacct acttttaggg tagcgggaag
    10141 cttttcattg aaagtaacga tctgataccg cccattgatg ttttcaatgg ctttcagggt
    10201 caagtggtca taggcaaaaa aggagtcgga caggcgaacc aggcctatgg tgtcatttgg
    10261 ggtgccgata ggaaagcctt gttgctgtgc atgccccatt ctgaccccaa tgctgttttc
    10321 gcttaagata tccactactt cctgataggt gccatgtacg ttcgtggcat cgtccaactg
    10381 attgttgaag gtgcagttct ttagtaccac tttcccgcgg catcccacga aatgggtggc
    10441 atctgcagag gtggaaacca ttctaccatg ggagggtttt acatgaaaac catctaagat
    10501 gaggtccgat gagttttcgg cgatcaggcc cataccgccg gcatggttta ccgttacgtt
    10561 atttgcgtta aaaccgtcgg ttccggaaat acggaaggca ggggccaacc ggtttaagct
    10621 ttggtcgcct ttacagacca agatcatgcc tacgggcggc agtttttttc tgtgattgta
    10681 tatgcgaacg gtaccaggtt ttatctcttt tacccttaaa ttatcttcct tgccccttac
    10741 tttttctact ttagagcgtg ggtcaacttt gtatttgtat tttattttat cgaagttgcg
    10801 ctgcgtcttt gaattacgta tcgttgtgat gggtgtgtac gcttctgtgt caaaggcaat
    10861 agcatttctt atggggtcgt atagaatgga ctggccaagg tcgtgctcat agtattcttt
    10921 tagaaagatg agttgttcat tcctaatctc atacggatat tcatctgaaa aatggatgtc
    10981 aaaagtgttg tttttttcgt cgttagctac tatagtaccc tcgctgtgaa aaggcatggc
    11041 ccaatctata gtaacattct gcaccgtaat gttttcagag ccttcaatta agaaagggat
    11101 cattctgcca tggtaaataa aggttgaacc ttgaccatca attactaggt ctttaaaacc
    11161 gagaaggggc aaggccgttt ttgccattac gtcgtcatgg ttcgagatgc gggtaaagcg
    11221 ttcaaaagcc ttatcgggat aaaagtgata ggtgcccttt tcaaatttta tttgggaaat
    11281 aggatggtct tttgcttgac gtatccgtgc tagaagggca ggggtggcat catcggcaat
    11341 atcggtttta aaaagtactt tttcgttgag ggatgccgat tgtgaaaatc ccataatacc
    11401 cacaagacat agggttaggg cgataaggtt tttataggga gatgtattct tcattttttg
    11461 attttaaagc ctttattgtt ttttgtttgc agtgttttgg tcgaggtcgg atcacttttg
    11521 atgatgtttt cgtttgtgcc cgaataactg ttctttataa tcttagtggc cttgcccttg
    11581 gtgatttcga acatgggggc gttcgggtat agctcggggg catccttggt ttgaataatg
    11641 gtattgcccg agacctccaa tccgtctacc cgatcggccc atacgatacg gtttccgaag
    11701 gtctgtatac ggttgttcac gaagcggata tttcggtcat agcgaatatt ttggtcaaag
    11761 cccttgccta gacgtggggt aatatagaga acggcgtgtt ccataccact ataggcgcaa
    11821 tattcaaaat tgttgttttg tatgagtacg tcttgtaccg cgccgctttc gaaccagaaa
    11881 aagctttctc cgcgaaagaa aacggaagac atcatagaag agaaattatt gttttcgata
    11941 accgttttta aaggggtctt cagtacaatg tttcgggccc tgtggtcttt aacggtgcag
    12001 ccgcgcatgg tgaaggtagg gttccaggtt ttgttctcca gaatatcgcc tacggccaat
    12061 tccgggggga gtccgtcgtt aaaggaaagg tcgatatact tgtcgtttac taccgatacc
    12121 gaagctacgg tatgaaccga acctcttttg gggttcggtt ggtgtataaa ccagatttca
    12181 tcattgggag cggcaaattc gaatcccatt tgctcaaaat gttggagtgc tacccttacc
    12241 gtccgttggt caatgatccg gtcgacgacc acataggtgc catgtacgtt cgtaccatcg
    12301 tcaagcatgt gtttaaaggt gcagttttct ataaggatat cacctttaca gttggcaaaa
    12361 tgtgtggcgt cggcaatggt cgagaccaca cggtcagacc cttctcgtac atacacgccg
    12421 caattgtcga ttgtaatgtt ttcggttcgt tcgaacaaga agcccattcc aagggcgtgg
    12481 tggaccttta tgttttttag gaggatgttt tgtgacgagg tcacctgaaa ggccggggca
    12541 tatcggttat ggtcatggtc cccctttgaa tggaggatgg agcctacaga agggtattgc
    12601 tttaaatgtt cgtaaaaacg aagcgttcca ttggggcgtt tttcaaccca acgtggtcta
    12661 ctgctcatgt cccaagcgcc atgggccacc cgcttgtgtt cggggtcaaa ggccaaggtg
    12721 cttcccattt cggggaagac gaagccgtct atatcgggaa aaaggatccg cccgttgttc
    12781 agtttccatg aatagccctc ggtgaagggc ttgatatcac gccagccttc cttttcgttc
    12841 actgcaacta cttcaccctg aaaggtaaaa gggatatccc agtctatact gaggtcgttt
    12901 accgtgactt tcttgcagtt tttgaactga aaaggggcta cttggccgtg gaagataaat
    12961 tcggccccat tgccttcaat ggtaaccgaa tcaaaatcct ccaacagaaa aataatattc
    13021 tttaatccat tgccgtgatt ggtgatatac gaatatcgtt gggtggcata atctggtaaa
    13081 aatttataaa cccctgtctc aaaaaccaag gtcaggtttt tatcgctcac attctctaag
    13141 gctttacgga ccacgggggt catatcttcg gcatcgggtt gaaattgaat ggtttgttgt
    13201 gccgtaaggt ggaatacccc taaaaggaat attacgaata catatgttga tttcatagat
    13261 ctaaaaaaat gtcggttttg tgcttagagg ttaactccgg tgccttgtaa agaattgtcg
    13321 tggtcccatg taatgagcca agggtctttg gtctcttttt gaaaagcttt cattttgact
    13381 tttaaggcct ctagaacgtc tttgtaattt gtgtcatccg ccaaattatg gctttcactg
    13441 gggtcttttg acaggtcgaa caattcaaat tcaggtctga agaggtagtc tttgaccttg
    13501 cgttttccaa agtattcttt ttcgcttcgg tatacttcct gccaagtaga tgcggcccaa
    13561 agatccgatg cgaagggata ttcttgacgc catgcgatat tgcggataag tttgaagttt
    13621 ttatccctga ctacccgcat ggggtagtac atggtaatct cgtggaaggt atgggaggca
    13681 tagatttcat cccatccctt tgggttttcc tcggcgagta tttttttgaa cgatttacca
    13741 tgaaaggcgt tggagtcata gctgacgttc gccatatcta agatggtggg agtaaggtcg
    13801 gtccacgata taagggcgtt gttgacagaa ccacccttca ggccggccag agggcctttt
    13861 ataatgcagg gcaatttaat gcccggctca taaacggtag tcttggcacc gggaaaggcc
    13921 atgccgttat ctgaaatata gatgactacg gtgttcttgt ccttgcccgt atcttttagc
    13981 attttcatga gctttccaaa accttggtct attcttgaaa cgctttggta gtattgtgct
    14041 atttcttctc gggtttcctt gctgtcggga aggaaatcgg gtacgaccac atctttgggg
    14101 tcataggtaa cggtctctac gccctcgtat ccttctttct tattgccgaa actgttggga
    14161 aggtaccaag ggtccgattg aaagggggcg ccacggtggg ggtcgtctgt acagaaatag
    14221 agaaagaaag gtttttcgga attaaggacg tccttgcact tctcggccat ttctacggta
    14281 ttccgtgggt cggcctccaa tactttttga aagtggtaaa ccgattcggg ggcaacgtgg
    14341 tattttccta ttcgagccgt ttcgtaacct gctttttcca gtaggacggg cagggattta
    14401 acattatcat agctgctaaa gtgatgatag tcgtgtacat gtccgtacga accggtagcg
    14461 tgcccatata gaccggtgag tataaccgat cggctggcgg cacagcttgc actggtgcaa
    14521 taggcattgt tgaaacgtac accttcggcg gccagttgat ctaggttggg ggttttgatc
    14581 actttgtttc cataggcgcc gatagcatct gtgccatggt cgtcggaaac aaagagaatg
    14641 atgttgggcg atgtgttttt cgcgtctttt ttagcaaggt cttttgtttt gtctttggtg
    14701 gtacagccaa caaatagtaa aagaaagata aggactgggg taaaaagttt tgaggtcatg
    14761 gtatgtcttt gattttgaaa cataaggcat tgccgtacaa agtagatata tattcttgat
    14821 ttctatgtat gcaagggttt atgaaatgta tttgggagta caaatgtggt tatagagaaa
    14881 aagtcattcc cgatgaaagg aatgactttt agactaaata gggtgaaggc taaggcctta
    14941 gtttaggtta gggtttagtg tagtttcggt agcgggaata ggggtaaacc ataggttttt
    15001 gtcggcggga tctccgatta agggaacctc ccttgcatca cgcgtggcat tcgcttccgc
    15061 aactttttct aaacggacca agtctaacca gcggttgccg ctaatttccc ctaagaattc
    15121 ataggccctt tcgttaacga tcgcatcgat catcaactgg ggagttgaaa gttcggcaag
    15181 tgtatagtcg atggccgggt cgggagtcat ccaaaatccg ggttccaagg cggctacttc
    15241 ggcttgtttg gtcgctcctt tggcataggc ccttcttttg accctgttca attcttccaa
    15301 ggcggtagct aggtctcctg tatttgcagc ggcttcggca tgcattagta aaacttccgc
    15361 ataacgcatg gcaatacggt cgttggaggt ttcccatgta tgttcaacga ccaggtcaag
    15421 gggatcggtt gaattggggg ccggtgtacc ccaggtatac ttggtgacta tagggttttt
    15481 gaattcttca aagaactgat ttccctctgt agcggtacaa aactcttttc ggtagccttc
    15541 agggaaattg ttaaaatagg tccactccat gtacaagtcc atccagttgg cccatctgta
    15601 accatatacc ctgttttggg taattattgg ccaagattcc ttagacataa tatgggcaaa
    15661 aatgacttcc ttgttgatct gatggggatc ttgaaagtta taggcaaaat catcggttag
    15721 ggaataaacg ccgtcagcaa taatttcacc cgtcagttgt tttacttgct cccatttatc
    15781 cgcttcattg tagggatagc cggtcaactg catatatgtt ttcgccaaga aggccttggc
    15841 cgcagttttt gaaaccctac cgttcgatat cgtgggattg gaatctctct cgttaacggc
    15901 ccatttaatg gcgaactcaa aatctttgat ggcttgggcc ataacctctc tcataggggt
    15961 taatggaatt tccaggtctg gaacgggatc caaaggcata ggcacatcgc caaaacttgt
    16021 tgcaagtctg aaatagcata ggcctctaat aaaataggcg ttggcatggg ccgcgtttat
    16081 tacggattcc tctacacctt ctgggtgggc atcggcgata tagctattgc aggccagaat
    16141 ggtttgatag ttcccattgt aatcattggt agcccacgag ttacctgcag tccttgcaaa
    16201 ttggtccgat tcaagaacga gccatttgtt gctgcccaat cttgaggtta ggtcatccgc
    16261 acccgtgaaa tgtgcataat tatctaagcc ccaagtattt ctacgtaggg tgccgtagat
    16321 gcctattata ccggcatcta atgtgttttc gtccgaatag aacgtcgatg aggcaactac
    16381 cccgtccgat ccttcgtcta acttttcaca acttccaaat cctagaagga taatgaaggc
    16441 aaagagtact gtatatgtat tataaattcg tttcattttc tttgtttttt gattaaaaac
    16501 ttactttgag tccgagggaa atagttcttg ttaatgggta tgaaaaggca tcgacacctg
    16561 attgggagtc actggatagt gccgtactcg aaccttcagg gtctagacca gaataattgg
    16621 tgatagtcag tatattgtct gaactaacgt atagcctgat actactgagc cctagggcgt
    16681 cggttgtatt atcgggtatg ttgtacccaa gggtcatgtt actgatcttg aagaaggagc
    16741 ctttttcgat atactggctt acgacatagg taagggagta gtccgttcca taaggcatca
    16801 cctgttgggt agatgaggtg ttggtaggtg accacctatt tctgtaatcc gttgaaagaa
    16861 taccatagtt caacctgttg tccgtccaat tatagatatc attacctacg gttccaatac
    16921 attgtatgga aaggtcgagg cctttgtagg cgatgcttgt accaagtcca tacgtaaaat
    16981 cgggagaagc gttaccgata atttgtcggt cttccgccga tatggctcca tcgttgtcta
    17041 tgtcctcata gcgcacttca cctggtctgg agctatacga atcggcttct acagcctcat
    17101 cttcttgcca aatacctatg gccttcaatc cgtagaacga gcccatagat tcaccttttc
    17161 ttacaatgaa tttattattg cccccaacta agctccatat gcctatgggc tcttcattgg
    17221 cgataaaaat ttcttctacg gattcacctc ccgattggcc taggttttga acgatatttt
    17281 tgttttttga gaaattggcg tacgcactcc attgaaaatc atctttgctc acgatattcc
    17341 aattaaggcc cacttcaata ccattgttgt ctatatcggc aaaattgtcg gtaatggagg
    17401 attgaccggt atatcggggt atggatttag cgatgattac atcagttgcc ttttttgaat
    17461 agacatctat ggtaccggtt aacctattgt ctagaatacc gaaatcgata ccggcgttat
    17521 aggaggtggt ggtttcccat tttaaatctt tatcggtaat agagccgggc acgacccctt
    17581 gaacggattc cccattgtct aattggtacc aaccataatt acgtccgtct ccactatcgg
    17641 aagctgcaaa tgtttgttga atggttccat aaccgaccgc ttggttcccc acggaaccga
    17701 aacctaacct aaatttcagg ttgcttacga agtcggaggt tttaaggaaa ttttcttcgg
    17761 aagccctcca tgcaaaagca gctgagggaa aataacccca tctgttgtcg gcgttaaatc
    17821 gagacgatcc atctgcacgc atagaagctg tgagtaggta acggtcctta aaactataat
    17881 taagacgtcc catatacgaa cggagttttc ctgtaccata gcctgaatta agtgacgagg
    17941 gagagccatt ggccaaaagg tgccattcgt tggctaaggt ggaaagattg ccgacggtgg
    18001 ccgtattgtt tcggtttatc gattttgaaa actcatatac gcccgatatt ttcaggttgt
    18061 gatcgccaaa gttgttttca taggtaagaa tactgttggc caaccagcta ttgtttctgc
    18121 cgtaggcttg ttgtgcggta atgttgtctc tacttatgtt gataaagtcc ggtagggttc
    18181 gctgaccgtg ataattcatt gagaggcttc cggcggcact tagcgataag gtcaaatggt
    18241 tggccacatc aatgttaagg tcaatgttgg aagtgatctt atcattgagg gtttcttggt
    18301 tttgcctaac ctgcatttcg gggtttatga gtcttcccac aagaaaagcc ccattatatc
    18361 cattataggt ggttccatct ataaattcac cgttggcgtc acgcgactcc cacatttggg
    18421 ggaatatcac ggcattaccg attgggtttg ccgtaccgcc cgtacccctt ccattggctt
    18481 gggattcgat atgggttcca taggtgttaa atttaaattg gatacgttct ccaatatttc
    18541 ctgatatatt ggaacggatg gaagttcttc tataaaatga attttttata acgccttcat
    18601 cgtttaaatg gttggcggag aaaaagtatt ttaaattttc agagccgccc gtaagattca
    18661 aggaatagtt ttgtttatat ccttggcgtg tgataaggtc gacccagtcg gttccgcctt
    18721 tagacctaaa accggcaatg tcctcagctg aaaagtaagg ggatttgttg agcgaagctt
    18781 gttgctcgtt cataagttcg gccatttcgt cgatgggtaa gatctcgtat ttattgacca
    18841 tggaactcat ggagatattg gaatcgaaag taacctttaa gggcccttcc ttcggggcga
    18901 cggttgttat caagaccacg ccattggcac ctcttgatcc gtacacggct gttgcggatg
    18961 catcttttag aaagtttatc gtggcgatgt cgttagggtt gatggttgaa atatcggccc
    19021 ctataaagcc atcgattaca tataaaggtt cgttgccata gtttatggaa cttccccctc
    19081 tgatacgtat tttcattttt tgacctggtg ctcccgtggt ttgttgaata tttaccccac
    19141 tgatctttcc ctgtagacct gccccgattt tggtaatggg cgccttgagt aggtctttgg
    19201 cggaaataga gcctaccgat ccggtaaggt cgcttttctt ttgtgtacca taacctacta
    19261 ccacgacctc atctagccct gcagcgtttt cttgcatcac tatttttagg gttttttggc
    19321 cttgtgtggc gatttgttgt gttgagaacc ctatatatga taccgttaat acggcattgg
    19381 ggcttttaac ggttatggaa aaatttccgt caaaatcggc agtgacaccg tttgaggttc
    19441 cttgttccaa gatgttggcc ccaggtaagg gtaaaccggt ttggtctacc accgtaccac
    19501 taacggtagt gtcttgggca taaatagttg atgttataag aaataacatc aagaatgata
    19561 cgcttatcct aagcgtatgt ttccaattaa taaatagttg atctcgcttc at
//
