LOCUS       CP028460               14035 bp    DNA     linear   BCT 22-OCT-2018
DEFINITION  Bifidobacterium animalis subsp. animalis strain CNCM I-4602
            chromosome, complete genome.
ACCESSION   CP028460
VERSION     CP028460.1
DBLINK      BioProject: PRJNA445301
            BioSample: SAMN08774047
KEYWORDS    .
SOURCE      Bifidobacterium animalis subsp. animalis
  ORGANISM  Bifidobacterium animalis subsp. animalis
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 14035)
  AUTHORS   Egan,M., Bottacini,F., O'Connell Motherway,M., Casey,P.G.,
            Morrissey,R., Melga,S., Faurie,J.-M., Chervaux,C., Smokvina,T. and
            van Sinderen,D.
  TITLE     Staying alive: Growth and survival of Bifidobacterium animalis
            subsp. animalis under in vitro and in vivo conditions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14035)
  AUTHORS   van Sinderen,D., Chervaux,C. and Smokvina,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2018) School of Microbiology, University College
            Cork, Western Road, Cork ND, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SMRT Analysis v. 2.3
            Expected Final Version :: yes
            Genome Coverage        :: 400.0x
            Sequencing Technology  :: Pacbio SMRT sequencing
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14035
                     /organism="Bifidobacterium animalis subsp. animalis"
                     /mol_type="genomic DNA"
                     /strain="CNCM I-4602"
                     /host="Homo sapiens"
                     /sub_species="animalis"
                     /db_xref="taxon:302912"
                     /country="France"
                     /collection_date="01-Oct-2014"
     gene            complement(1..1242)
                     /locus_tag="CNCMI4602_0058"
     CDS             complement(1..1242)
                     /locus_tag="CNCMI4602_0058"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8G3Q9|3.A.1.2.22"
                     /protein_id="AYN22928.1"
                     /translation="MTTANATPAPAKAPAPKKDAKGNGVLSTLANNSKQYGIVGALIVI
                     VLVFQFLTDGVLLKPNSFVSLIQQNAYVIILAIGMVMVIIATHIDLSVGSIVAFVGGVC
                     AILMERQGVNWMLAILISLLVGLVIGCWQGFWVAYVGIPGFITTLAGMLIFRGLATVIV
                     GESVPVTSPEFRGIARNYLPNILGWWGPFDGLTIVVGIICIALYAWSLLRRRAKVAKAG
                     LNPEPMALTVTKIVIATLLIGFVTYLLALSGNATQGGIPIMLVILAVLVFIYNFILTKT
                     VFGRHVYAVGGNRKAAILSGINTKRVDFTLFVHMGFLSAVAAVCVLSRLASATAQAGME
                     FEMDAIAACFIGGTAVTGGVGTIPGAVVGALVMGVINQGLSIMGVDTAIVKTIKGLVLL
                     GAVAVDIMTKRKKS"
     gene            complement(1239..2795)
                     /locus_tag="CNCMI4602_0059"
     CDS             complement(1239..2795)
                     /locus_tag="CNCMI4602_0059"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8G3R0|3.A.1.2.22"
                     /protein_id="AYN22929.1"
                     /translation="MPHNDTILTMNNIVKTFGPVKALSGVDLTVGRGEIHAICGENGAG
                     KSTLMNVLSGVYPYGSYSGTITYNGKECKYKTINDSEADGIVIIHQELALSPFLSIAEN
                     IFIGNEQATHGMIDWDETRAKAKALLEKVGLPEDPDTKIMDIGVGKQQLVEIAKALSKD
                     VKLLILDEPTAALNDEDSAHLLNLVRQLRDEQGVTSIIISHKLNEIADIADNVTIIRDG
                     ATVGEMFITPETPLDQDELIRKMVGRSLTNLYPYHESNAGGEEYLRIEDWTVHHPLDQN
                     RVIVDHANINVHSGEIVGLAGLMGAGRTELAMSVFGKSYGSRISGKVFVKGKEVHLPNV
                     QAAIDAGLAYATEDRKVYGLNLLQNIRENAALASLGKMSSHGIMDENGERTEVEEYRND
                     FHIKCSNIDVNVGTLSGGNQQKVVLAKWVISNPDILILDEPTRGIDVGAKYEIYEIINK
                     LADEGKAIIVISSELPELIGICDRIYTVSQGVVTDNVNKNGFTQEYLMKGMTKEKEVVA
                     S"
     gene            complement(2825..4000)
                     /locus_tag="CNCMI4602_0060"
     CDS             complement(2825..4000)
                     /locus_tag="CNCMI4602_0060"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8G3R1|3.A.1.2.22"
                     /protein_id="AYN22930.1"
                     /translation="MMKSTKRIIALISAIAICAGLGACSSTRAGFEQTGGTQKLEKGAT
                     IGISMPTKSEERWNKDGNNLKDKLEKAGYKVVLNFADDKPAQQNADIENMVNQNAKILV
                     VASKDGSAVGPAVEKARDAGATVIAYDRLIMNTDAVNYYATFQLEQVGKLEAEYLVDQL
                     GLNDGQKGPFNMELFTGSPDDNNAKYFFKGAWEVLQPYFAKGVLVNPSKHGGGVTKDFK
                     VDDWQKISVQSWKTEQAQKDMESILDSTYASGRKLDAVLTPYDGIAQGVINAIESKRPD
                     MQPGTESWPPITGQDAMEIAVSNIAAGKQGETVFKDVNKLADAVYDMIIEIAEGKQVTG
                     INGKFNNNVVDVPSKLLNPQNITGENLDELVKAKYITQERFDKLTKGEDVR"
     gene            4217..4825
                     /locus_tag="CNCMI4602_0061"
     CDS             4217..4825
                     /locus_tag="CNCMI4602_0061"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|MarR,DBD-SUPERFAMILY|0040517"
                     /protein_id="AYN22931.1"
                     /translation="MLLDMAGRFSKKKLGSQSSLREANRANLLESIHKFGAMTQVELAE
                     IMGVSTATISALVHQLVDEGQLETQGTVRNGRRAQLVTLAHHQGLAAGVHITRRGLRVV
                     LCDDTRTPFADHYYPLPQRHLPDTTLDVVIRLIRESLDQMGAQPSELFAIGVALGAPSI
                     RAHTCLPCPDCSPAGRRWTSAPCWNRRSACLCWWTTMRI"
     gene            4771..5427
                     /locus_tag="CNCMI4602_0062"
     CDS             4771..5427
                     /locus_tag="CNCMI4602_0062"
                     /codon_start=1
                     /transl_table=11
                     /product="NagC/XylR family repressor"
                     /protein_id="AYN22932.1"
                     /translation="MLESAFGVPVLVDNDANLSAFCEARVGAGAGIEDFVYVNASDGVG
                     AGIVEGGEIRRGITGLAGEIGHIQVDPLGSICQCGNRGCLNTVVDEQRLTSLLAVTHGN
                     LTLNDLVAKANEGDPGCRRVIADAAVRIGNVTSTLCISVDPELVVVGGELARAGDVFLE
                     PFRESLQRLLFPDALTPISVLEAKMPKISAALGAGILAMEGVERASINGGTQSEG"
     gene            5467..6318
                     /locus_tag="CNCMI4602_0063"
     CDS             5467..6318
                     /locus_tag="CNCMI4602_0063"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O50504|3.A.1.2.24"
                     /protein_id="AYN22933.1"
                     /translation="MSVQSTLIELHGVTKKYGNVEALREVSLAVRRRQIVAIVGDNGAG
                     KSTLVHVIAGLEQPDSGSITICGRQVRIDGIAKAAELGIASAFQQPEFCENLDVSANIF
                     LGQELRRGIAARDDTGMYDKAREVLNTLSTPVRASQSISSLSGGQRQTVAIARTLLNDP
                     SLVVLDEPTASLSVTQTAEVLEYITELRSANRSVIMVCHDLPNVFAIADRIVVMRHGEI
                     RAVHNTSETSYEEVIAEISGVDRPRTPLPRLRERASRTRTQVGREPRSRPSRGGDHDGT
                     IA"
     gene            6535..6876
                     /locus_tag="CNCMI4602_0064"
     CDS             6535..6876
                     /locus_tag="CNCMI4602_0064"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AYN22934.1"
                     /translation="MGAAEIVVTVVATIACLGMGVFLGSLPFKRDELTEWHVKYQQLCG
                     VLACGIVIILMLLGYDLASWLAIAALAVGVAVSRIPVVRRGLLARFPELHPAERQSPLR
                     KGASKKRRS"
     gene            complement(6871..7905)
                     /locus_tag="CNCMI4602_0065"
     CDS             complement(6871..7905)
                     /locus_tag="CNCMI4602_0065"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="AYN22935.1"
                     /translation="MAKGVTIKDVAAEAGVSFKTVSNVINGTGSMRESTRGRVLEAMDK
                     LNYTINLSARSLKTGTTGLLGLAIFDFSQPFASYLADQIIVCAREHHYGVIINTYGQNE
                     HGLARAMRQANNLAADGWIVFADHAMGQHSKMLDQSYPLVLTGDWDAYGKVDQVTMPNV
                     EAMRYTTNRLLDSGYRSIALFGADGSLGARHYRQATEGTQELRVQGYMQAYEEHGIEAR
                     MDMLFSGGLLTSDSGVRATNLMMDQGVRPDAIICLNDAMALGALHQLQVRGIRVPDDVQ
                     VVGFDNVPEATYANPSLTTIDPHIDSYARHAVDMLIERIQGYDGAKRVYTSDFTLVERS
                     STSL"
     gene            complement(8023..9564)
                     /locus_tag="CNCMI4602_0066"
     CDS             complement(8023..9564)
                     /locus_tag="CNCMI4602_0066"
                     /codon_start=1
                     /transl_table=11
                     /product="GH51"
                     /protein_id="AYN22936.1"
                     /translation="MAEHTPAHARLVVDDEFRVGPVHDRVFGSFVEHLGRCVYTGIYEP
                     GHPTADAEGFRQDVIDLVKELGATTIRYPGGNFVSGYRWEDGIGPKENRPRRLDLAWHS
                     TETNQFGLHEMVSWLNKTGGNELMEAVNLGTRGLPEALDLLEYANVPEGTARSEERIRN
                     GADNPFDIRMWCLGNEMDGPWQLGHKDAEDYGKLAASVAAGMRMLDPDLELVVCGSSSH
                     TMDSFGKWEETVLEHTFDKVDFVSAHAYYFPQLMENGSRDMASFLASGVDMDGFIKDVG
                     AAIDATKSRLKSDHNVYISFDEWNVWYQEEEPSKNPEGIGNWPVAPRLLEDVYTVADAV
                     VVGDLLITLLQNADRVHAASLAQLVNVIAPIMTEPNGPAWRQTTFYPFSLTAAWAKGGD
                     VLEPKIESAQYHTNRYGNVNSTNAVAVRGKDGSVSVFVTNRSLDDGCDFEIKLPEGFTP
                     TELDVRTLHDDDMLATNTVNHQDRVVPHPNDTASIQDGMVTVTLPPVSWTAIHLR"
     gene            9901..11514
                     /locus_tag="CNCMI4602_0067"
     CDS             9901..11514
                     /locus_tag="CNCMI4602_0067"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="AYN22937.1"
                     /translation="MADSKAIAQAIRDGRTSLGIEFGSTRIKAVLIDDERNTITTGDYG
                     WQSHLVDGLWSYDVDKIWRGLQSVYADVADRVEADYGLKLTRIGQIGFSAMMHGYLAFD
                     ADGELLVPFRTWQNTNTSEAHEKLSELFQYNIPERWSIAHLYQAVLDNEPHIGSVRYFT
                     TLSGYVHWKLTGRKVLGIGDASGMFPIDPNTKTYEQGFIEQFDALPEVAAQPWKLNDLL
                     PEPLVAGTPAGELTPEGAALLDPSGVLQPGTMLAPPEGDAGTGMVATNSVRRRTGNVSA
                     GTSIFATVVLEHKLSKLRPEVDLVTTPAGDLAGMSHANNFTADLNGWVDLFRQFSQLNG
                     RNVDDGTLYGELFRAAIGEDADPDAGGLLAYPFRTGEFLAGLAEGRPLFVRGPEARFSL
                     ANFMRAQLFGAFSPVTIGMNVMTEDEGVEIDSLVGHGGIFATPVVAQRILAAAFNTPIR
                     VMTTASEGGAWGMAVLADYLRHADLTLADYLDDVVFAGAESVTEAPRPDDVDGFQRFFA
                     RFVKALPVERSAVEHVEIGG"
     gene            11570..12262
                     /locus_tag="CNCMI4602_0068"
     CDS             11570..12262
                     /locus_tag="CNCMI4602_0068"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="AYN22938.1"
                     /translation="MATLADYGPEVRAEVKQVREVVAALHEQLIKWNLVVWTAGNVSQR
                     LRTADLMVIKPSGLRYEYLTPSSMVVCDLDGNVVDGAESPSSDTASHAYIYRHMPEVYG
                     VVHTHSTYATAWAATGQNIPCGLTMMGDEFGGPVPVGPFRLIGSEAIGEGVVETLKAYP
                     KSPAVLMQNHGPFTIGKDAEAAVKAAAMTEEVAHTMWAAKQLGDIIPIPQEDIDKLNDR
                     YQNVYGQH"
     gene            12518..14035
                     /locus_tag="CNCMI4602_0069"
     CDS             12518..14035
                     /locus_tag="CNCMI4602_0069"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="AYN22939.1"
                     /translation="MAMENPFEGKEIWFGVGSQDLYGEEALRQVAQQSAEIVDSLNASG
                     RIPVKLVLKPTLKSSDGVRRFMVDASADDSCIGVITWMHTFSPAKMWIRGLEALRKPLL
                     QLNTQHHFEIPWETIDMDFMNLNQAAHGDREYGYIVTRLGIPRKIVVGHYTDPAVADKI
                     GVWARACAGWQASNTMNVMRWGDNMRNVAVTEGDKTEAERVFGASINTWAVNELVAAYD
                     AVKENQIKDLIEDYKAKYEIAPELLDKDYDSLFIAAREECAMVNMMRANGCTAGVDNFE
                     DLGALPQLPGVGPQRFPSEYGWGFSAEGDWKTAVLVRIGAVMGYGLEGGASLMEDYSYN
                     FTPGNEMIMGSHMLEVSPSVGTIAKPRLEIHPLGIGGKADPVRLVFTVAPKKDAVVVSL
                     ADVRTRFRMLMNVVDVVEPLGSLDKLPCARALWKPEPNLEISAECWLRAGGSHHTCMTT
                     SVGREAWEDFARIAGVELATIDAETTPREFERELEIEDMFHELANKH"
ORIGIN
        1 tcagctcttc ttgcgcttcg tcatgatgtc gacggcaacc gcaccgagga gcacgaggcc
       61 tttgatcgtc ttgacgattg cggtgtcgac gcccatgatc gacaggccct ggttgatgac
      121 gcccatgacg agtgcgccaa ccactgcgcc gggaatggtg ccgacgccgc cggtgactgc
      181 cgtgccgccg atgaagcagg ctgcgatcgc atccatctcg aactccatgc cggcttgtgc
      241 ggttgccgag gcgaggcggg agagcacgca gacggctgcc actgccgaaa ggaagcccat
      301 atgcacgaag agtgtgaaat cgacgcgctt cgtgttgatg cctgagagga tggccgcctt
      361 gcggttgccg cccacggcat agacatgacg cccgaacacc gtcttcgtca gaatgaagtt
      421 gtagatgaac acgagcaccg caagaatgac gagcatgatg ggaatgccgc cttgggtcgc
      481 gttgcccgac agtgccagca ggtaggtgac gaagccgatc agcagcgtcg cgatgacgat
      541 cttcgtgacg gtcagcgcca tcggttcggg attgaggccg gccttggcga ccttggcacg
      601 ccgacgcagc aagctccacg catacagcgc gatgcagatg atgccgacga ctatggtcaa
      661 tccgtcgaat ggaccccacc agccgaggat gttcggcagg tagttacgtg cgatgccgcg
      721 gaattccggg gaggtgaccg gaacggattc gccgacgatg accgttgcga ggccacggaa
      781 gatgagcatg cccgccagtg tggtgatgaa gcccgggatg ccgacgtacg ccacccagaa
      841 gccctgccag cagccgatga ccaatccgac gagaagcgag atgagaatgg ccagcatcca
      901 gttgacgcct tgtcgttcca tcaatatcgc gcagacaccg cccacaaagg cgacaatcga
      961 accgacggac aaatcgatat gggtggcgat gatgaccatg accatgccga ttgccagaat
     1021 gatcacgtag gcgttctgct ggatcagaga gacgaagctg ttcggcttca acaggacgcc
     1081 atcggtgagg aactggaaca cgagcacgat gacgatcagc gcgcccacga tgccgtattg
     1141 ctttgaattg ttggccaatg tgctcaggac gccattgccc ttcgcatcct tcttcggtgc
     1201 cggcgccttt gccggtgctg gcgttgcatt tgcggtggtc atgatgcaac aacctccttc
     1261 tctttcgtca tgcctttcat caggtattcc tgtgtgaagc cgttcttgtt cacgttgtcg
     1321 gtgaccactc cctggctcac ggtgtagatg cggtcgcaga tgccgatcag ttccggcagc
     1381 tccgacgaga tcacgatgat cgccttgcct tcatcggcga gcttgttgat gatttcgtag
     1441 atctcgtatt tggcgccgac gtcgatgccg cgggtgggct cgtcgaggat caggatgtcg
     1501 ggattcgaga tcacccattt cgcgagcacg accttctgct ggttgccgcc cgacagggtg
     1561 ccgacattga cgtcgatgtt gctgcacttg atgtggaagt cgttgcggta ctcctccact
     1621 tcggtgcgtt cgccgttctc gtccatgatc ccgtgcgagc tcatcttgcc cagcgaggcg
     1681 agtgccgcgt tctcacgaat gttctgcagc agattgaggc cgtagacctt gcggtcttcc
     1741 gtagcgtacg ccagcccggc atcgatcgcc gcctgcacgt tcggcagatg cacctccttg
     1801 cctttgacga acaccttgcc ggagattcgc gatccgtacg acttgccgaa cacgctcatc
     1861 gcgagctcgg tgcggcctgc gcccatgagt cccgccaggc ccacgatctc tcccgaatgc
     1921 acgttgatgt tcgcatggtc gacgatgacg cggttctggt cgagcgggtg atgcaccgtc
     1981 cagtcctcga tgcgcagata ttcctcgccg ccggcgttcg actcgtggta tgggtacaga
     2041 ttcgtgaggg acctgccgac catcttcctg atcagctcgt cctggtcgag cggggtctcc
     2101 ggcgtgatga acatctcacc gaccgtcgcc ccgtcgcgaa tgatcgtcac gttgtcggca
     2161 atgtcggcga tctcgttgag cttgtggctg atgatgatgc tcgtgacgcc ttgctcgtcg
     2221 cgcagttggc gcaccagatt caacagatgc gccgaatcct cgtcgttcaa ggccgcggtg
     2281 ggttcgtcga ggatcagcag cttgacgtcc ttcgacaacg ctttcgcgat ttcgacgagc
     2341 tgctgcttgc ccacgccgat gtccatgatc ttcgtgtccg gatcttcggg cagaccgacc
     2401 ttctccagca gcgccttggc cttcgcacgc gtctcgtccc agtcgatcat gccgtgggta
     2461 gcctgttcgt tgccgatgaa gatgttctcc gcgatcgaca ggaacgggct cagcgcgagt
     2521 tcctggtgga tgatgacgat gccgtcggct tcggaatcgt tgatggtctt gtacttgcac
     2581 tccttgccgt tgtaggtgat cgttccggaa taggacccgt aggggtagac gccggacagc
     2641 acgttcatca gtgtggattt tccggctccg ttctcgccgc agatcgcgtg gatctcgccg
     2701 cgccccaccg tcagatccac gcccgacagc gccttgaccg ggccgaacgt cttgacgatg
     2761 ttgttcatcg tcagaatcgt gtcgttatgt ggcatgacct gctccttccg cacgggatgc
     2821 cgcgtcagcg gacgtcctcg cctttcgtga gcttgtcaaa ccgttcctgc gtgatgtatt
     2881 tcgccttgac cagctcatcg agattctctc cggtgatgtt ctgagggttg agcagcttgg
     2941 atggcacgtc gacgacattg ttattgaatt tgccgttgat gccggtgacc tgcttgcctt
     3001 cggcgatttc gatgatcatg tcgtacaccg catccgcgag cttgttgaca tccttgaaca
     3061 ccgtttcgcc ttgcttgccg gctgcgatgt tcgacaccgc gatctccatc gcgtcctgac
     3121 cggtgatcgg aggccagctc tcagtgcccg gctgcatgtc gggtctcttc gattcgattg
     3181 cgttgatgac gccctgtgca atgccgtcgt atggggtcag caccgcgtcg agcttgcgtc
     3241 cgctggcgta ggtggagtcg aggatcgatt ccatgtcttt ctgtgcctgc tcggtcttcc
     3301 acgactgcac cgagatcttc tgccagtcgt ccaccttgaa gtcctttgtg acgccgccac
     3361 cgtgcttgga ggggttcacg agcacacctt tggcgaagta tggctgcagc acttcccagg
     3421 cgcccttgaa gaagtatttc gcattgttgt cgtcgggcga accggtgaac agttccatgt
     3481 tgaacgggcc cttctggccg tcgttgagcc cgagctggtc gacaaggtat tctgcctcga
     3541 gtttgcccac ctgttcgagc tggaatgtgg cgtagtagtt gacggcatcg gtgttcatga
     3601 tcagtcggtc gtatgcaatc accgtcgcgc cggcatccct ggccttctcg acggccggtc
     3661 cgaccgccga accgtccttc gaggcgacga cgaggatctt cgcgttctgg ttcaccatgt
     3721 tctcgatgtc ggcattctgc tgtgccggct tgtcgtcggc gaagttcagc acgaccttgt
     3781 agccggcctt ctcgagcttg tctttgaggt tgttgccgtc tttgttccag cgttcctcgg
     3841 atttcgtggg cattgagatg cctatggtag cgcctttttc gagcttctgc gtgccgccgg
     3901 tctgttcgaa accggcacgg gtggagctgc acgccccgag acccgcgcat atcgcaatgg
     3961 cgctgatgag ggctatgatc cttttggtgc tcttcatcat ctgctccatc tcctttgacg
     4021 gaatgtgatt ggtggcttca tcgccgcgat ctcgttgaga tctagttcca tatcatacga
     4081 tgttgagtta agttgtcaaa ctcaacatgg cgtgttgtgt cgttttgagt tcaggcaata
     4141 aatccaaaac gatgttttcg ccatcatttt gtggcatttc tcaccgattt gcttcgattt
     4201 cgagggtttt ggatttatgc tgttagatat ggcaggacga ttctcgaaga agaagcttgg
     4261 ctcccaaagc tccctccgcg aggcgaatcg cgccaatttg ctggaatcga tccacaaatt
     4321 cggggcgatg acgcaagtcg agctcgccga aatcatgggc gtgtcgaccg ctacgatctc
     4381 ggcgctcgtg catcaattgg tggatgaggg gcagctggag acgcagggca ccgtgcgcaa
     4441 cgggcgacgt gcccagttgg tgacgctcgc gcaccatcag ggacttgcgg ccggggtgca
     4501 catcacacga cgtggattgc gcgttgtgct gtgcgacgat acgcgtaccc cgtttgcaga
     4561 ccactactat ccgttgcccc aacggcatct gcccgacacg accctggacg tggtgattcg
     4621 cctcatccgc gaatccctgg accagatggg agcgcagccc agcgagctgt tcgccatcgg
     4681 ggtggccttg ggtgccccgt cgattcgcgc tcacacatgc ttgccgtgcc cggattgctc
     4741 cccggctggg aggaggtgga cgtcggcgcc gtgctggaat cggcgttcgg cgtgcctgtg
     4801 ctggtggaca acgatgcgaa tctgagcgcc ttctgcgagg cgcgtgtggg agccggtgcg
     4861 ggcattgagg acttcgtgta tgtgaacgca agcgacggtg tcggggccgg catcgtggaa
     4921 gggggagaga ttcgccgtgg gattaccgga ttggcagggg agatcggcca tattcaggtg
     4981 gacccgctcg gatcgatctg ccaatgcgga aatcgcggtt gcctgaacac cgtggtcgat
     5041 gagcagcgat tgacctcgct gttggcggtc acccacggca atctcacatt gaacgatctc
     5101 gtggccaagg cgaatgaggg cgacccgggt tgccgccgtg tgattgccga cgcggcggtg
     5161 cgcattggca acgtgacctc gacgctgtgc atctcggtcg accccgaact cgtcgttgtt
     5221 ggcggcgagt tggcgagggc cggcgacgtg tttctcgaac cgttccggga atccctgcag
     5281 cgcctgctgt tcccggatgc cctgacgccg atctcggtgc ttgaggcgaa gatgccgaaa
     5341 atcagcgccg ctttgggggc ggggattctg gcgatggagg gggtggagcg tgcgtcgatc
     5401 aacggtggaa cgcagtccga aggataatct ggaaggcgga gagctcgcac gggaatgggg
     5461 aaggcaatga gtgttcagtc gacgttgatt gaactgcatg gcgtaacgaa gaagtacggg
     5521 aacgtggaag cgttgcggga ggtcagcctc gcggtgcgga ggcggcagat cgttgcgata
     5581 gtgggagaca acggcgccgg caaatcgaca ttggtgcatg tcatcgccgg actcgagcag
     5641 ccggattccg gcagcataac gatatgcggc aggcaagtgc gtatagacgg tatcgcgaag
     5701 gcggccgagc tcggcatagc atctgcgttc cagcagcccg agttctgcga gaatctggat
     5761 gtctcggcga atattttcct cgggcaggag ctgcggcgag gcattgctgc gcgcgatgac
     5821 accggcatgt acgacaaagc gcgcgaagtg ctcaacacgc tatccacgcc ggtgcgcgcc
     5881 agccaaagca tatcctccct ttcgggaggg cagcggcaga ccgtggcgat tgcgcgcacg
     5941 ctgctcaatg atccgagtct ggtcgtactt gacgagccga cggcgtcgct gtcggtgacg
     6001 cagaccgccg aggtgctcga atacatcacc gagttgagat cggcgaatcg cagcgtcatc
     6061 atggtctgcc atgatctgcc gaacgtgttc gcaatcgcgg atcgcatcgt cgtgatgcga
     6121 cacggggaga tccgggccgt gcacaacacg tcggagacct cctacgaaga ggtcatcgcg
     6181 gagatctccg gtgtggacag gccccgcacc ccgctgcctc ggttgcgtga acgggcgtcg
     6241 cgcacgcgaa cacaggtcgg tcgtgagccg cgctcgcgcc cgtcacgagg aggggaccac
     6301 gatggaacca ttgcgtgaaa gaagtgagcc attgcgtaaa aagagacact cctcggacgt
     6361 atatgtccct tattacgcag atttgttgcg cagattgaat acgcaatccg cgcattcgag
     6421 cttctctcaa tcttgcgtaa ttggagacaa atcacagctg attttgtctc tttttacgca
     6481 ggatgagaag caagaagcaa tgaagtaaga aggaaaagaa aggaagggaa tggcatgggt
     6541 gcagcggaaa tcgtagtcac cgtggtggcg acaatcgcat gcctgggcat gggcgtgttt
     6601 ctggggtcat tgccgttcaa gcgagacgaa ctgacggaat ggcatgtcaa ataccaacag
     6661 ctatgcggcg tgctcgcctg cggcatcgtg atcattctga tgctgctcgg ctatgatctg
     6721 gcatcgtggc tcgcaattgc ggctctcgca gtgggtgtgg cggtgagtcg cattccggtg
     6781 gttcggcgag gcttgctggc gcgtttcccc gagctgcatc cggccgaacg gcagtcgccg
     6841 ttgcgcaagg gcgcgtcaaa gaagcgccgg tcatagactg gttgacgagc gctccaccaa
     6901 ggtgaaatcg gatgtgtata cgcgtttcgc gccatcgtac ccttgaatgc gttcgatgag
     6961 catgtccacc gcgtgccgcg cgtagctgtc gatatgcggg tcgattgtcg tcagagacgg
     7021 gttcgcatac gtcgcctccg gtacattgtc gaaacccacc acctgcacat cgtcgggtac
     7081 gcggattccc cgtacctgca attgatgcaa cgctcccagc gccatcgcgt cattgagaca
     7141 gatgatggcg tccggtcgca cgccttggtc catcatcaga ttcgttgcgc gaacgccgct
     7201 gtcgctcgtc aataagccac ctgagaacag catgtccatg cgggcctcga tgccatgctc
     7261 ctcgtatgcc tgcatatacc cttgcacgcg gagttcctgc gtaccctcgg tggcttgtcg
     7321 gtaatgcctt gcgccgagcg agccgtctgc gccgaacagc gcgatggaac ggtacccgga
     7381 atcaagcagg cgattggtgg tgtatcgcat cgcctccacg ttcggcatgg tgacctgatc
     7441 caccttgcca taggcgtccc agtcgccggt gagcaccaga ggatagcttt ggtcgagcat
     7501 cttgctgtgt tgtcccattg catgatcggc gaagacgatc cacccgtctg cggccagatt
     7561 attcgcctgt cgcatcgccc gtgccaggcc gtgctcattc tggccgtagg tgtttatgat
     7621 gaccccgtaa tgatgctccc gcgcgcagac gatgatctga tcggccagat acgaggcgaa
     7681 tggctgggag aaatcgaaga tggccaatcc gagcaacccg gttgtgccgg ttttcagtga
     7741 acgtgccgag aggttgatcg tgtagttcag cttgtccatt gcctcaagca cgcgtccacg
     7801 tgtcgattca cgcatgctgc cggtgccgtt gatcacgttc gagactgttt tgaacgatac
     7861 gccggcctcg gccgcgacat ccttgatggt cacgcctttt gccatggtcg gtctccgatg
     7921 cgccttgctg tttacctatg cattgcctat cgccttgaat cgtatcacta tgcacgttgc
     7981 cggggatgcc ggtggcccag accgacggtc cgggccgttg tgtcagcgca gatggattgc
     8041 ggtccaactc accggcggaa gggtcacggt gaccatgcca tcctggatgg aggcggtgtc
     8101 gttggggtgg ggcaccacgc gatcctgatg gttcacggtg tttgtggcca gcatgtcgtc
     8161 atcgtgcaac gttctcacat caagctcggt aggcgtgaaa ccttccggca acttgatctc
     8221 gaagtcgcat ccatcgtcca gcgaacggtt ggtgacaaac acggagaccg agccgtcttt
     8281 gcctcgcacg gccacggcat tcgtggagtt cacgttgcca tagcggttgg tgtggtactg
     8341 ggcggactcg atcttgggct ccagcacatc gccgcccttg gcccaggctg cggtgaggga
     8401 gaacgggtag aaggtggttt ggcgccaagc gggcccattc ggctcggtca tgatgggggc
     8461 gatgacgttg acgagctgag cgagtgatgc cgcatgcaca cgatctgcgt tttgcagcaa
     8521 tgtgatgagg aggtcaccca ccaccacggc atcggccacg gtgtagacgt cctcgagcag
     8581 ccgaggggct acgggccagt tgcctatgcc ttccgggttc ttgctgggtt cctcctcttg
     8641 gtaccacaca ttccactcat cgaaggaaat gtacacgttg tggtcgctct tgagtcgcga
     8701 cttcgtggcg tcgatggcgg cgcccacatc cttgatgaat ccatccatat ccacgccgga
     8761 ggcgaggaac gacgccatgt cgcgcgagcc gttctccatc agctgcggga aatagtaggc
     8821 atgtgctgaa acgaaatcca ccttgtcgaa ggtgtgctcg agcacggtct cctcccattt
     8881 gccgaaagag tccatcgtat ggctcgatga accacagacg accagctcaa gatcggggtc
     8941 taacatgcgc atgcctgccg ccaccgaggc cgcgagcttg ccgtagtcct cggcgtcctt
     9001 gtgcccgagc tgccaagggc cgtccatttc attgcccaag caccacatgc ggatgtcgaa
     9061 cgggttatcg gcgccgttgc gaatacgttc ctccgagcgg gcagtgcctt ccggaacgtt
     9121 cgcatattca agcagatcca gtgcttctgg caatccgcgg gtgccgagat tgacggcttc
     9181 catcaactcg ttgccgccgg tcttattcag ccatgagacc atttcgtgca gcccgaactg
     9241 attcgtttcg gtggaatgcc aagccagatc aaggcgacga ggtcggttct cctttgggcc
     9301 aatgccatct tcccatcgat agcctgacac aaagttgccg ccggggtagc ggatggtggt
     9361 ggcaccgagt tccttgacca gatcgatgac gtcttggcgg aacccctcgg catcggcagt
     9421 gggatgcccc ggttcataga tgccggtgta tacgcagcgt cccagatgct cgacaaatga
     9481 gccgaacaca cggtcgtgca ccgggccgac cctgaattcg tcatctacca cgagtctggc
     9541 atgtgctggg gtatgctcgg ccatattgcc tcctgttcct cggtgtcgca ctaagtacaa
     9601 cgttgttctt ttgtgcaagg gtccttcgtc gtcgaagaag gattggggtt gggtttgaag
     9661 gttgggttag tacaaccttg tacttcatgg taccgctcat attcggtcgg tggggacgaa
     9721 agggtggcgt gggtcggttg acgcttggga tgacgccgtt tgctcggcgt gttggtgtgg
     9781 tggcgttcgc tcagaacaat tgaagtttgg tttccttggc gtgttatcgg ttgtgtgagc
     9841 gctaacatat acatgacgca tgagttcatt gcagaactga atcccgaagc aggaggaacc
     9901 atggcagatt caaaggcgat tgcacaggcc attcgtgacg ggcgcacgtc gttgggcatt
     9961 gaattcggtt ccacgcgcat caaggcggtg ctcatcgacg atgagcgcaa tacgatcact
    10021 accggtgact atggttggca gtcacatctt gtggacggcc tgtggtcgta tgacgtggac
    10081 aagatctggc gcggattgca gagtgtctat gccgatgtgg ccgaccgcgt ggaggccgat
    10141 tacggcctca agctcacgcg catcggccag attggcttct ccgcgatgat gcatgggtat
    10201 ctcgctttcg atgcggatgg tgaactgctt gtgccgttcc ggacctggca gaacaccaac
    10261 acgagcgagg ctcatgagaa actctccgag cttttccaat acaatattcc ggaacgttgg
    10321 tcaatcgcgc acctgtatca ggcggtactc gacaatgagc cgcatatcgg ttccgtgcgc
    10381 tatttcacca cgctttccgg ctatgtgcac tggaagctca ccggtcgcaa ggtactgggc
    10441 atcggtgatg cgtccggaat gttcccgatt gatccgaaca ccaagaccta tgagcaggga
    10501 ttcattgaac agttcgatgc gctgcctgaa gtcgccgcac aaccgtggaa gctcaacgat
    10561 ttgcttcccg aaccgcttgt tgcgggcacc ccggccggcg aactcacgcc ggaaggtgcg
    10621 gcgctgctcg acccgtctgg cgtacttcag ccgggaacga tgctcgctcc tccggaaggt
    10681 gatgccggaa ccggcatggt ggccacgaac tcggtacgcc gtcgtaccgg caacgtgtct
    10741 gcgggaacct cgatcttcgc caccgttgtg cttgagcaca aactctccaa gttgcgcccc
    10801 gaagtggact tggtgaccac ccccgcgggc gatctggccg gtatgagcca tgcgaacaac
    10861 ttcaccgccg atctcaatgg atgggtggac ctgttccgcc agttctcaca gctcaacgga
    10921 cgaaacgtcg atgacggcac cctgtacggc gagctgttcc gcgcggccat aggcgaggat
    10981 gcggatccag atgccggtgg tctgctcgcc tacccattcc gtaccggcga atttctcgcc
    11041 gggctcgccg aggggcgtcc gctgttcgtg cgggggcccg aggcgcgatt ctcgctcgcc
    11101 aacttcatgc gggcacagct gtttggcgca ttctcgccgg tgaccattgg tatgaacgtg
    11161 atgaccgagg acgagggcgt cgagattgac tcgctggtgg gccatggtgg catcttcgcc
    11221 acgcctgtgg tggcgcagcg tattctcgcg gcggcgttca atacgccgat ccgtgtgatg
    11281 accaccgcgt ccgaaggggg agcgtggggc atggccgtgc ttgcggacta tttgcgtcac
    11341 gccgatctca cgcttgcaga ttatttggac gatgtcgtgt tcgccggggc ggaatcggtg
    11401 accgaagctc cccggccgga cgatgtggat ggattccagc gattcttcgc cagattcgtc
    11461 aaggcgttgc cggtggagcg cagtgccgtt gagcacgtcg aaatcggtgg gtgaatctgc
    11521 agcgtacgga tctgcgcaag tatttgaaac tgtacagaaa ggaacaacaa tggcaacact
    11581 ggcggattat ggaccggaag tccgtgcgga ggtcaagcag gtgcgggagg tggtcgccgc
    11641 actgcatgaa cagctcatca agtggaatct ggtggtctgg acggcaggga acgtttcgca
    11701 gcgtctgcgc acagctgacc tcatggtaat caaaccatca gggctgcgtt acgaatatct
    11761 gacaccgagc tcgatggtcg tgtgcgacct cgacggcaac gtcgtcgacg gtgcggaatc
    11821 accatcgtcg gacactgctt cacatgcata catctaccgc cacatgccgg aggtgtatgg
    11881 cgtggtgcat acccattcca cctatgcgac cgcatgggcc gccaccgggc agaatattcc
    11941 atgcggattg acaatgatgg gcgacgagtt cggtggtccg gtgccggtgg ggccgttccg
    12001 tctgatcggt tcggaagcca ttggcgaagg tgtggtcgag accctcaagg cgtacccgaa
    12061 atcaccggcc gtgctcatgc agaatcacgg cccattcacc atcggcaaag atgcagaggc
    12121 cgcggtcaag gccgccgcga tgaccgagga ggtggcgcat acgatgtggg cggccaagca
    12181 gctcggcgac atcattccga ttcctcagga agacatcgat aaactcaatg accgttacca
    12241 gaatgtgtat ggccagcact gacttgcacc atcacaatac aactgcgtaa acaaattcaa
    12301 ctgcgtaaga agagacagat tcgcatggtt tttgtctcga attacgcaag gtagcaaggc
    12361 ggcaaggtag caagttggtt tgcgtaaaaa gggacagaaa ctgcggttat tcgtcccgga
    12421 ttacgcaagg gcgggcaaac agctgagtca tccacacatc aatccacatc ggttcataat
    12481 caattccaac aatccacaat gaagcgagga aaacaacatg gcaatggaaa atccgttcga
    12541 aggcaaggag atctggttcg gcgtcggttc gcaggacctg tacggcgagg aggcactgcg
    12601 acaggtggcg caacagtccg ccgagattgt cgattcgctc aacgcgagtg gccgcatccc
    12661 cgtcaagctt gtacttaagc ccacgctgaa gtcgtctgac ggcgtgcgtc gcttcatggt
    12721 ggacgcctcg gccgatgatt catgcatcgg cgtgatcacg tggatgcaca ccttctcgcc
    12781 ggcgaagatg tggatccgtg ggctcgaggc gctgcgcaag ccattgctgc agcttaacac
    12841 ccaacaccat ttcgagattc catgggagac cattgacatg gatttcatga atctcaacca
    12901 ggccgcgcac ggtgatcgtg aatacggcta cattgtcact cgcctcggca tcccgcgcaa
    12961 gatcgtcgtg gggcattaca ccgatcccgc ggtggccgac aagatcggcg tgtgggcgcg
    13021 cgcctgcgcc ggttggcagg cctcgaacac gatgaacgtg atgcgctggg gcgacaatat
    13081 gcgcaacgtt gccgtcaccg aaggcgacaa gaccgaggcg gagcgtgtat ttggcgcctc
    13141 gatcaacacc tgggccgtca acgagctcgt cgccgcgtac gatgcggtca aggagaacca
    13201 gatcaaggac ctcatcgagg actacaaggc gaagtacgag atcgcgcccg agctgctgga
    13261 caaggactat gattcgctgt tcatcgccgc tcgtgaggaa tgcgcaatgg tgaacatgat
    13321 gcgtgccaat ggctgcacgg cgggcgtcga caacttcgag gacctcggcg cgttgccgca
    13381 gctgcctggt gtggggccgc agcgcttccc atccgaatat ggctggggtt tctctgccga
    13441 aggtgactgg aagacggcag tgctcgtgcg catcggggcc gtgatgggct acggtctcga
    13501 gggtggtgcg tcgctcatgg aggactactc ctataacttc accccgggca acgagatgat
    13561 catgggatcg cacatgcttg aggtctcgcc gtcggtgggc acaatcgcca agccgcgtct
    13621 cgagatccat ccgctcggca tcggcggcaa ggccgatccg gtccgtctgg tgttcaccgt
    13681 ggctccgaag aaggatgcgg tcgtggtctc gctcgccgac gtccgtactc ggttccgtat
    13741 gctcatgaat gtggtcgatg tggtcgaacc gctcggctcg ctcgacaagc tgccatgcgc
    13801 ccgtgcgctg tggaagccgg agccgaatct ggagatttcc gccgaatgct ggctgcgtgc
    13861 aggcggctcc caccacacgt gcatgaccac ctcggtcggc cgtgaggcat gggaggactt
    13921 cgcgcgcatc gccggcgtcg aactcgcgac gatcgacgcc gagaccacgc cgcgcgaatt
    13981 cgaacgcgaa ctcgagattg aggacatgtt ccacgagctc gccaacaagc actga
//
