LOCUS       U00096                  4071 bp    DNA     linear   BCT 24-SEP-2018
DEFINITION  Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION   U00096
VERSION     U00096.3
DBLINK      BioProject: PRJNA225
            BioSample: SAMN02604091
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1462 (1997)
   PUBMED   9278503
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Hayashi,K., Morooka,N., Yamamoto,Y., Fujita,K., Isono,K., Choi,S.,
            Ohtsubo,E., Baba,T., Wanner,B.L., Mori,H. and Horiuchi,T.
  TITLE     Highly accurate genome sequences of Escherichia coli K-12 strains
            MG1655 and W3110
  JOURNAL   Mol. Syst. Biol. 2, 2006 (2006)
   PUBMED   16738553
REFERENCE   3  (bases 1 to 4071)
  AUTHORS   Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
            Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
            Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
            Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
  TITLE     Escherichia coli K-12: a cooperatively developed annotation
            snapshot--2005
  JOURNAL   Nucleic Acids Res. 34 (1), 1-9 (2006)
   PUBMED   16397293
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4071)
  AUTHORS   Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
            Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
            Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
            Wanner,B.L.
  TITLE     Workshop on Annotation of Escherichia coli K-12
  JOURNAL   Unpublished
  REMARK    Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE   5  (bases 1 to 4071)
  AUTHORS   Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
            Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
  TITLE     ASAP: Escherichia coli K-12 strain MG1655 version m56
  JOURNAL   Unpublished
  REMARK    ASAP download 10 June 2004 (annotation updates)
REFERENCE   6  (bases 1 to 4071)
  AUTHORS   Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
  TITLE     A more accurate sequence comparison between genomes of Escherichia
            coli K12 W3110 and MG1655 strains
  JOURNAL   Unpublished
  REMARK    GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE   7  (bases 1 to 4071)
  AUTHORS   Perna,N.T.
  TITLE     Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
            sequence correction
  JOURNAL   Unpublished
  REMARK    GenBank accession AY605712 (sequence corrections)
REFERENCE   8  (bases 1 to 4071)
  AUTHORS   Rudd,K.E.
  TITLE     A manual approach to accurate translation start site annotation: an
            E. coli K-12 case study
  JOURNAL   Unpublished
REFERENCE   9  (bases 1 to 4071)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   10  (bases 1 to 4071)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   11  (bases 1 to 4071)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   12  (bases 1 to 4071)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2004) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   13  (bases 1 to 4071)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein updates by submitter
REFERENCE   14  (bases 1 to 4071)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database collaboration
REFERENCE   15  (bases 1 to 4071)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2013) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Sequence update by submitter
REFERENCE   16  (bases 1 to 4071)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   17  (bases 1 to 4071)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2013) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein update by submitter
REFERENCE   18  (bases 1 to 4071)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2014) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein update by submitter
COMMENT     On Sep 26, 2013 this sequence version replaced U00096.2.
            Changes to proteins and annotation made on September 24, 2018.
            Current U00096 annotation updates are derived from EcoCyc
            https://ecocyc.org/.  Suggestions for updates can be sent to
            biocyc-support@ai.sri.com. These updates are being generated from a
            collaboration  that includes EcoCyc, the University of Wisconsin,
            UniProtKB/Swiss-Prot, and the National Center for Biotechnology
            Information (NCBI).
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     gene            complement(1..909)
                     /gene="melR"
                     /locus_tag="b4118"
                     /gene_synonym="ECK4111"
                     /db_xref="ASAP:ABE-0013486"
                     /db_xref="ECOCYC:EG11230"
                     /db_xref="EcoGene:EG11230"
     CDS             complement(1..909)
                     /gene="melR"
                     /locus_tag="b4118"
                     /gene_synonym="ECK4111"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0043644,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607"
                     /protein_id="AAC77079.1"
                     /db_xref="UniProtKB/Swiss-Prot:P0ACH8"
                     /db_xref="ASAP:ABE-0013486"
                     /db_xref="ECOCYC:EG11230"
                     /db_xref="EcoGene:EG11230"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWHG
                     QVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHLFL
                     SWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLMLKR
                     FSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVKLNAN
                     YAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYSTFGKY
                     VGMSPQQYRKLSQQRRQTFPG"
     gene            1192..2547
                     /gene="melA"
                     /locus_tag="b4119"
                     /gene_synonym="ECK4112"
                     /gene_synonym="mel-7"
                     /db_xref="ASAP:ABE-0013493"
                     /db_xref="ECOCYC:EG10577"
                     /db_xref="EcoGene:EG10577"
     CDS             1192..2547
                     /gene="melA"
                     /locus_tag="b4119"
                     /gene_synonym="ECK4112"
                     /gene_synonym="mel-7"
                     /EC_number="3.2.1.22"
                     /codon_start=1
                     /transl_table=11
                     /product="GH4"
                     /protein_id="AAC77080.1"
                     /db_xref="UniProtKB/Swiss-Prot:P06720"
                     /db_xref="ASAP:ABE-0013493"
                     /db_xref="ECOCYC:EG10577"
                     /db_xref="EcoGene:EG10577"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTR
                     LEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVCKR
                     HGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYAR
                     YPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNL
                     YPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGR
                     EDLIERYKVPLDEYPKRCVEQLANWHKELEEYKKASRIDIKPSREYASTIMNAIWTGEP
                     SVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLL
                     TEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR"
     gene            2650..4071
                     /gene="melB"
                     /locus_tag="b4120"
                     /gene_synonym="ECK4113"
                     /gene_synonym="mel-4"
                     /db_xref="ASAP:ABE-0013495"
                     /db_xref="ECOCYC:EG10578"
                     /db_xref="EcoGene:EG10578"
     CDS             2650..4071
                     /gene="melB"
                     /locus_tag="b4120"
                     /gene_synonym="ECK4113"
                     /gene_synonym="mel-4"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A7ZUZ0|2.A.2.1.1"
                     /protein_id="AAC77081.2"
                     /db_xref="UniProtKB/Swiss-Prot:P02921"
                     /db_xref="ASAP:ABE-0013495"
                     /db_xref="ECOCYC:EG10578"
                     /db_xref="EcoGene:EG10578"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVG
                     TLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTT
                     QIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVT
                     LPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLV
                     TLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTAL
                     FWVLQVIMVADIVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPN
                     VEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEP
                     VHADIPVGAVSDVKA"
ORIGIN
        1 ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt gcggcgacat
       61 gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc ctgccgtcag
      121 ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg
      181 catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata tccccattgc
      241 atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg cctgatcata
      301 gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc gcgagacgct
      361 gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagccag agaggctaaa
      421 tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct cgttcggact
      481 gtttaattcc tgctgccagc ggcgcacttc aaacgggcta agttgctgtg tggccagtga
      541 tttgatcacc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg gccaggagag
      601 aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg tatctgttag
      661 ttggtgcggt gtacaggccc agaacagcgt gatatgaccc tgattgatat tcactttttc
      721 attgttgatc aggtattcca catcgccatc gaaaggcaca ttcacttcga cctgaccatg
      781 ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc gctggtattc
      841 cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca taaacgtatc
      901 tgtattcatg gatggctctc tttcctggaa tatcagaatt atggcaggag tgagggagga
      961 tgactgcgag tgggagcacg gttttcaccc tcttcccaga ggggcgaggg gactctccga
     1021 gtatcatgag gccgaaaact ctgcttttca ggtaatttat tcccataaac tcagatttac
     1081 tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc ggaggttttc
     1141 tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg catgatgtct
     1201 gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa tattcttggt
     1261 gatgtgttcc atcgcgaggc gctgaaaacg gcgcatattg ccctgatgga cattgacccc
     1321 acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc aggggccagc
     1381 ggcaaaatca cctgccacac ccaacagaaa gaagccttag aggatgccga ttttgtcgtg
     1441 gtggcatttc agattggcgg ttatgaacct tgcacggtga ctgatttcga ggtctgtaag
     1501 cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat tatgcgcgcg
     1561 ctacgtacca ttccgcatct gtggcaaatt tgcgaggaca tgacggaagt ctgccccgat
     1621 gccaccatgc tcaactatgt taacccaatg gcgatgaata cctgggcgat gtatgcccgc
     1681 tatccgcata tcaaacaggt cgggctgtgc cattcggtgc agggaacggc ggaagagttg
     1741 gcgcgtgacc tcaatatcga cccagctacg ctgcgttacc gttgcgcagg tatcaaccat
     1801 atggcgtttt acctggagct ggagcgcaaa accgccgacg gcagttatgt gaatctctac
     1861 ccggaactgc tggcggctta tgaagcaggg caggcaccga agccgaatat tcatggcaat
     1921 actcgctgcc agaatattgt gcgctacgaa atgttcaaaa agctgggcta tttcgtcacg
     1981 gaatcgtcag aacattttgc tgagtacaca ccgtggttta ttaagccagg tcgtgaggat
     2041 ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt cgagcagctg
     2101 gcgaactggc ataaagagct ggaggagtat aaaaaagcct cccggattga tattaaaccg
     2161 tcacgggaat atgccagcac aatcatgaac gctatctgga ctggcgagcc gagtgtgatt
     2221 tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg ttgcgtggaa
     2281 gtagcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac gctaccttcg
     2341 catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac cgaagctatt
     2401 cttacggaaa atcgcgaccg tgtttaccac gccgcgatga tggacccgca tactgccgcc
     2461 gtgctgggca ttgacgaaat atatgctctt gttgacgacc tgattgccgc ccacggcgac
     2521 tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc cgggggattt
     2581 attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt aacagcgacc
     2641 cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg agcgttcggg
     2701 aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac cgatgtcgtc
     2761 gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg ggatgctatt
     2821 aacgatccga ttatgggatg gattgtaaat gctacgcgat cgcgatgggg taagttcaaa
     2881 ccctggatcc tgatcggtac gttggcaaac tctgtaatct tatttctcct ctttagtgcg
     2941 catctgtttg aaggtactac tcagattgtc tttgtttgcg tgacctacat cctctggggc
     3001 atgacttaca ccattatgga tattcccttc tggtcgctgg ttccaaccat cacgctcgat
     3061 aaacgtgagc gcgaacaact ggttccttat ccgcgttttt ttgccagtct ggcaggcttt
     3121 gttacggcag gtgtgacgct accatttgtt aattatgtcg gcggtggcga tcggggattt
     3181 ggctttcaga tgttcactct ggtactgatc gcctttttta ttgtttcaac catcatcact
     3241 ctgcgcaatg tgcatgaagt cttttcgtca gacaatcaac cgtctgctga aggaagccat
     3301 ctgacactta aagccatcgt tgcgctaatt tataaaaacg atcagctttc atgcctcttg
     3361 ggtatggctc ttgcttataa tgtagccagc aacattatta ccggctttgc tatctattat
     3421 ttctcatatg ttatcggtga tgcggatttg ttcccctatt atctgtcgta tgcgggagct
     3481 gctaacctgg tgacgttagt attcttccca cgcttagtta aatcattatc ccgacgcatt
     3541 ttatgggccg gagcatctat tcttccggtg ttaagctgtg gtgttctcct gttaatggca
     3601 ttaatgagct atcacaacgt cgtcctcatt gtgattgcgg gtattttgct gaatgtggga
     3661 acggcgcttt tctgggtatt acaggtcatc atggtggcag atatcgttga ttacggtgaa
     3721 tataaactgc acgtacgctg tgaaagtatc gcttactccg tgcagactat ggtggtgaag
     3781 ggcggttcag cctttgcggc ttttttcatt gcggttgtgt tagggatgat tggctatgta
     3841 ccgaatgttg aacagtctac gcaagcccta ttaggtatgc agtttattat gattgctcta
     3901 ccaactctgt ttttcatggt aacgctgatt ctctacttcc gtttctatcg cctcaatggt
     3961 gacacgctgc gcaggatcca gatccatctg ctggataaat atcgcaaagt accgcccgag
     4021 cctgttcatg ctgatattcc ggtcggtgca gtgagtgatg tgaaagcctg a
//
