LOCUS       NZ_GG692727             9529 bp    DNA     linear   CON 20-DEC-2019
DEFINITION  Roseburia intestinalis L1-82 Scfld14, whole genome shotgun sequence.
ACCESSION   NZ_GG692727
VERSION     NZ_GG692727.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00008856
            Assembly: GCF_000156535.1
KEYWORDS    WGS; RefSeq.
SOURCE      Roseburia intestinalis L1-82
  ORGANISM  Roseburia intestinalis L1-82
            Bacteria; Firmicutes; Clostridia; Clostridiales; Lachnospiraceae;
            Roseburia.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B.,
            Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C.,
            Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C.,
            Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M.,
            Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2009) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B.,
            Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C.,
            Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C.,
            Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M.,
            Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2009) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   3  (bases 1 to 9529)
  AUTHORS   Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B.,
            Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C.,
            Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C.,
            Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M.,
            Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2009) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   4  (bases 1 to 9529)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2008) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2019 04:46:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 4,283
            CDSs (total)                      :: 4,204
            Genes (coding)                    :: 3,739
            CDSs (with protein)               :: 3,739
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 2, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 8 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 467
            CDSs (without protein)            :: 465
            Pseudo Genes (ambiguous residues) :: 39 of 467
            Pseudo Genes (frameshifted)       :: 133 of 467
            Pseudo Genes (incomplete)         :: 320 of 467
            Pseudo Genes (internal stop)      :: 22 of 467
            Pseudo Genes (multiple problems)  :: 46 of 467
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            GG692727.
            Roseburia intestinalis L1-82 (GenBank Accession Number for 16S rDNA
            gene: AJ312385 is a member of the division Firmicutes in the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from the laboratory of Prof. Harry Flint,
            Rowett Research Institute, Aberdeen, Scotland, UK
            (H.Flint@rowett.ac.uk).
            We have performed one round of automated sequence improvement
            (pre-finishing), along with manual improvement that includes
            breaking apart any mis-assembly, and making manual joins where
            possible. Manual edits also are made where the consensus appears to
            be incorrect. All low quality data on the ends of contigs is
            removed. Contigs are ordered and oriented where possible.
            This work was supported by the National Human Genome Research
            Institute and the NIH Roadmap Human Microbiome Project
            [http://nihroadmap.nih.gov/hmp/].
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Genome Coverage: 15.8X
            Sequencing Technology: 454.
            Roseburia intestinalis L1-82 (GenBank Accession Number for 16S rDNA
            gene: AJ312385) is a member of the division Firmicutes in the
            domain bacteria and has been isolated from human feces. The
            sequenced strain was obtained from the laboratory of Prof. Harry
            Flint, Rowett Research Institute, Aberdeen, Scotland, UK
            (H.Flint@rowett.ac.uk).
            We have performed one round of automated sequence improvement
            (pre-finishing), along with manual improvement that includes
            breaking apart any mis-assembly, and making manual joins where
            possible. Manual edits also are made where the consensus appears to
            be incorrect. All low quality data on the ends of contigs is
            removed. Contigs are ordered and oriented where possible.
            This work was supported by the National Human Genome Research
            Institute and the NIH Roadmap Human Microbiome Project
            [http://nihroadmap.nih.gov/hmp/].
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were
            blasted against NCBI's non-redundant (NR) database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.0. Gene names are generated at the contig level and may not
            necessarily reflect any known order or orientation between contigs.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Genome Coverage: 15.8X
            Sequencing Technology: 454
            Annotation was added to the contigs in August 2009.
            Product names were updated in June 2013.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Roseburia intestinalis L1-82"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld14"
                     /strain="L1-82"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Roseburia intestinalis"
                     /db_xref="taxon:536231"
     gene            complement(1..3927)
                     /locus_tag="ROSINTL182_RS11930"
                     /old_locus_tag="ROSINTL182_07683"
                     /db_xref="GeneID:43319281"
     CDS             complement(1..3927)
                     /locus_tag="ROSINTL182_RS11930"
                     /old_locus_tag="ROSINTL182_07683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM27|GH26|CBM23"
                     /protein_id="WP_044999186.1"
                     /db_xref="GeneID:43319281"
                     /translation="MRKRFMGKRLAAALLAAAVAFSSGNFAGGYEYVYAAEASVFYTGT
                     NTVTKDKLDLATEDWDNPPMITYDESIADCVTTKDFTMKADITLDDEAYASLGTGESYL
                     KVQGIVKLGSEWTWTDSQDIPYLQQSSFSEDTHKTTITIKFEDKDADDLKGVYFRLIGQ
                     GFSGTCTFSNVTLSGVAEAQPELPAKDPSVIDDFESAETGSSAGWEQEGGWQYDNAVGI
                     SVAEFQGSKMLKADLDYTGCEGFTWSEAKIKKSFAEGLDVSAYNVLSYEMIYPEAFDGS
                     FKAKIFAKNGADDVEIINKEAKIETSDLGDGYKKAVVTVKFSPNTAKITDLMIGTVGVS
                     TAFLGSVYLDNLTLSQYNAAGDYTEITETAGEAVLADTSSMPEEVTLSDVNASGSAKAL
                     YAYLKGLDAADQVLFGHQNDTSKHVSTRDGVYSDTKDVTGSISGLVGIDSLALTGVELG
                     IDNVDDAVQKSIEISKAAAAEGAIITLSTHMPNMSNEKIIATPDAARKYDFSQCDFSEA
                     KDLSNNCSAEVLPGGKYNAQFTTYLDIIADYANGLGDIPVLFRPFHENTGGWFWWGAAT
                     TDKETYRALFQYTQDYLASKGVHNFIYVYSPNGPLTSEEEYMDRYPGDDCVDIVAFDYY
                     DDYNTYPAEYSGEFVDNLKKTCQVVKNIADAKGKVAAISETGVRVMKADGSDNEGILVK
                     GNPIAGHNWYKQVNQVAVDTGMPYFLLWANFGDTNFYVPYKYDDTHGQELINEFIEFYN
                     EKSSVFANGTNFYGNADQKEVRNVNQGNAAGYFANVFSKMAITSAFTLKANVRNAQSVS
                     FVLTNPDTNTEKTITAEKTEESGAYEGVLSADILSALGTTDTGKISLVADGKTLVTVSY
                     ISFGKEKETLAKNVIENFELYYDDNDYLNGTFTENSAANCSSSFVLDKDHKAEGVYGGA
                     FTYQLKTGGPEVWTGRMKGLSTNDYSEYNAVSMWVKPDGKGQKLVVQLVSGGEDFEVFL
                     TDFVKTTEAKYVTIPFNKLKGKQNGTFDPKNVTKFAIWCNSISEDGNGVDIQSKIVFDD
                     IRFVNVDESKLNVTEGGYVLTDQSLVSGGQKPGDTEKPGDTEKPGDTEKPGDTEKPGNT
                     EKPGDTEKPGNTDKTTVAKVSGFKQSRATETSIRLTWKKVKKADGYQVYRYNKSTKKYQ
                     KVATVKTNTYTDKKLKTATVYQYKVRAYRKSGKKTVTGKYSNVIKAATSPQKVTAVKAK
                     RVKSKVKLTWKKVKGADGYEIYRATSKKGKYQKIKTLKKGTIVSYTDGKAKKGKTYYYK
                     VRAVKNAGKTSVKGTASNAVRCGK"
     gene            complement(4120..6924)
                     /locus_tag="ROSINTL182_RS11935"
                     /old_locus_tag="ROSINTL182_07684"
                     /db_xref="GeneID:43319282"
     CDS             complement(4120..6924)
                     /locus_tag="ROSINTL182_RS11935"
                     /old_locus_tag="ROSINTL182_07684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3"
                     /protein_id="WP_006857766.1"
                     /db_xref="GeneID:43319282"
                     /translation="MGNSGIGVPLPELAAYCREAAAEGAVLLKNEGHMLPIKKDETVSI
                     FGRSQIEYYRSGTGSGGAVNVPYVKNILDGIKENNAFPVNEDLVETYKEWLKEHPFDNG
                     GGGWAAEPWHQEEMEITDEIARRAAEKSEKAIFLIGRTAGEDKDYEDTEGSYLLTKREK
                     ENLRVVTKYFNEVAVLLNVSNIIDMSWTKDAAYQDHIKAILYIWQGGMEGANAVADLLS
                     GRVTPSGKLTDTIAEKLSDYPAADHFGSKTENIYAEDIYVGYRYFETFAPEKVMYEFGF
                     GLSYTEFSMEMVKAESTGNGKDAKIALSIRVKNTGAAAGKEAAQVYVSAPQGQLGKPAR
                     VLCGFAKTKLLAPGEEEVLELTIPVSRFASYDDSGVTGHKSCYVLEEGLYKIYVGNSVR
                     CTEKANVDGKGGYEVSSCIVTEELEEALAPTKEFLRLKTGRQKEDGVFARAYEKAPQQM
                     VDLAERIKSRLPKELPQTGNKGITLQAVAENIKNGSSVEEELDAFVAQFTNEELAVIVR
                     GEGMSSPKVTPGTASAFGGVSDSLHGYGIPIACASDGPSGIRMESGLKATQLPIGTLLA
                     CSFNIPMMEELYQMEGRELVGNEIDTLLGPGINIHRYPLNGRNFEYFSEDPLVTGQFAA
                     AMTRGIRSAGSSATVKHFAANNQETERHNVNSVVSERALREIYLKGFEIAVKEGNANSI
                     MTSYNPVNGHWTASNYDLNTTILRGEWGYQGIVMTDWWAKMNDVVNGGEADRRYTSFMV
                     RAQNDLYMVVNNNGAAINAAGDDTVEALEAGKLTVGELQRCAKNICRFLLGTPVMKRPL
                     KDFDPLLTVTAKEAVNTDEKQVCALQSGNRIEAGKFENTVLYVEKESILNVNAHFCFPV
                     EGLAQGAANLYLNGELMATVQTGGTGERFLTQRMCRVKFMPGYYELKAEYVTSELKLDW
                     IELE"
     gene            complement(7112..9529)
                     /locus_tag="ROSINTL182_RS11940"
                     /old_locus_tag="ROSINTL182_07685"
                     /db_xref="GeneID:43319283"
     CDS             complement(7112..9529)
                     /locus_tag="ROSINTL182_RS11940"
                     /old_locus_tag="ROSINTL182_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3"
                     /protein_id="WP_006857767.1"
                     /db_xref="GeneID:43319283"
                     /translation="MEKWQRSLYQPNLPLGADGTKVTASKAHITLSKEAAKEGMVLLKN
                     NESLLPFQAGTKLALFGKGSFDYVKGGGGSGDVTVAYTTNLYEGFKKLPEKVEVYEALS
                     DYYRKEVEKQYEAGAEPGMTVEPAFPEETAKKARAYTDTAVICISRFSGEGWDRKSSYD
                     KEMDESVQTDPLLEKAERIFPDGDFYLTKEESAMVEQVQQLFPKVAVVMNVGGMVDTDW
                     FAENEKIQAVLMAWQGGMEGGSAAAELLCGVGSPSGKLSDTFAKKLEDYPSSETFHESV
                     KYVDYKEDIYVGYRYFETIPGADKAVNYPFGYGLSYTTFERALVSAEEKQGVIRVRVNV
                     TNTGKYPGKEVMQLYAQAPQGVLGKAKRVLAAFEKTRLLAQGETQLLTLEAPVAQLASY
                     DDLGKIQKSAYVLEKGKYQFYLGTSVRETEQVFCFTMPEDTVTEQLTAKLVPTLLAERM
                     LSDGSFEKLPQSEPNDPDYSAIKRVPREESDGFSPAVRALPGHQIWSQPYKKDAHIFME
                     VAEGKITLDEFVTQMTDEELAHILGGQPNTGVANTFGFGNMPEYGIPNIMTADGPAGLR
                     IEKKCGVVTTAWPCATLLACTWNPDMLYQVGAAGAAEVKENNIAVWLTPAVNIHRSPLC
                     GRNFEYYSEDPYLTGKMASAMVKGIQSKHIAATVKHFACNNKETNRKESDSRVSERAAR
                     EIYLKAFEIIVKEADPWCIMSSYNIVNGHRTSENRELLEDILRGEWNYQGMVTTDWWTS
                     GEHYKEVKAGNDIKMACGFPESLLRAKEAGVLTREEMEICAKRILGLILKID"
ORIGIN
        1 ttattttcca cagcgcactg catttgaagc agttcctttc accgatgttt ttccggcatt
       61 ttttacggct cttactttat agtaatatgt ttttcctttc ttcgctttac cgtctgtgta
      121 agaaacaata gtgccctttt tcagcgtctt tatcttttga tattttcctt ttttggatgt
      181 tgcacggtag atctcgtatc catctgcacc ttttaccttc ttccaggtta attttacctt
      241 ggacttcaca cgttttgcct taaccgcagt caccttctgc gggctggtcg cagccttgat
      301 cacattggag tactttccgg ttaccgtctt tttaccgctt ttcctgtatg cacgcacttt
      361 atactgataa accgttgctg ttttgagctt tttatccgta taggtattag tttttaccgt
      421 tgcaaccttc tgatattttt tcgttgattt gttataacgg tatacctgat aaccatctgc
      481 tttctttact tttttccatg ttaagcggat ggaagtctcc gttgccctgg actgtttgaa
      541 gccggaaacc tttgcaactg tcgttttatc tgtattaccc ggtttctctg tatctcccgg
      601 tttctctgta tttcccggtt tttctgtatc tcccggtttc tctgtatctc ccggtttctc
      661 tgtatctccc ggtttctccg tatctcccgg tttctgtcct ccggacacga gtgactgatc
      721 tgtcagtaca tatccacctt ctgtcacatt taactttgat tcatccacat ttacaaagcg
      781 gatatcatca aaaacaattt tcgactggat atcaacccca tttccgtctt ctgaaatgct
      841 gttacaccag attgcaaact tcgtaacatt ctttggatca aaagttccat tctgtttacc
      901 ctttaatttg ttaaatggga tcgtaacata ttttgcctct gtcgtcttta caaagtcagt
      961 cagaaatact tcgaaatcct ctccgccgga aacaagctga actaccagtt tctgtccttt
     1021 tccatccggt tttacccaca tgctgaccgc attgtattcg ctgtaatcgt ttgtgcttaa
     1081 acctttcata cgtccggtcc atacttccgg tccgcccgtc ttaagctgat aagtaaatgc
     1141 acctccgtaa acaccttctg ctttatgatc cttatctaat acaaaggaag aactgcagtt
     1201 tgctgctgaa ttttctgtaa aagttccatt caggtagtca ttgtcatcat agtataattc
     1261 aaaattttca atgacattct ttgcaagtgt ttccttttct tttccaaaac tgatatagga
     1321 aacagtcaca agtgttttgc catctgccac aaggctgatc ttaccggtat ccgttgttcc
     1381 aagtgcactc agaatatccg cagacagcac accttcgtaa gcaccggatt cttccgtttt
     1441 ctctgccgtg atcgtttttt ctgtatttgt atccgggttt gtcagcacaa aacttacact
     1501 ctgtgcattt ctcacatttg cttttaaagt aaatgcactt gtgattgcca tcttagagaa
     1561 aacattggca aaataacctg ctgcatttcc ctgatttaca tttcttactt ctttctggtc
     1621 tgcatttccg taaaagtttg ttccattcgc aaaaacagaa gatttttcat tgtagaattc
     1681 aataaactcg ttgatcagtt cctgtccgtg ggtatcatca tatttatatg ggacataaaa
     1741 gttggtgtct ccaaaatttg cccacagtaa gaagtacggc attccggtgt caactgccac
     1801 ctgattaacc tgtttgtacc agttgtgtcc tgcgatcgga tttcctttta ctaaaattcc
     1861 ttcattatcc gatccatcgg ccttcatcac ccttacaccg gtttcggaaa ttgcagctac
     1921 ttttcctttt gcgtctgcaa tattctttac cacctgacag gttttcttta aattgtccac
     1981 aaactcgccg gaatattctg ccggataagt attgtaatca tcatagtaat caaatgcaac
     2041 gatatccaca cagtcatcgc ccggatagcg gtccatatac tcttcctctg aagttaacgg
     2101 accatttggg gaatatacat aaataaagtt gtgtactccc tttgatgcca gataatcctg
     2161 tgtgtactgg aacagcgcac ggtatgtctc tttatcggtt gtcgcagcac cccaccagaa
     2221 ccatccgcct gtattttcat ggaatggacg gaataaaaca ggaatatcgc caagtccgtt
     2281 tgcgtaatct gcaatgatat caagataggt tgtaaactgc gcattgtatt ttccacccgg
     2341 aagtacttct gcggagcagt tattgctcag gtcttttgcc tccgaaaaat cacactgtga
     2401 aaaatcatat tttctcgccg catccggtgt tgcaatgatt ttttcattgc tcatattcgg
     2461 catatgggtg gataatgtaa tgattgcacc ctctgcagct gctgccttgc tgatctcgat
     2521 agatttctga actgcatcat ctacgttatc aattccaagt tccacgcctg tcagtgccag
     2581 cgaatcgatt ccgaccagac cactaatact tccggtcacg tcctttgtat ccgaatagac
     2641 accgtctctg gttgaaacat gcttgcttgt gtcattctga tgtccgaata atacctggtc
     2701 agcagcatcc agtcctttta aatatgcata taacgctttt gctgaacctg atgcattcac
     2761 atccgaaagt gttacttctt ccggcatact ggacgtatct gcaagcacag cctctcctgc
     2821 cgtctctgtg atctccgtat aatcgcccgc tgcattatac tgggataaag tcaggttatc
     2881 cagatatacg cttcccaaaa aggcagtgct cacaccaact gttccaatca tcagatccgt
     2941 gatctttgca gtgtttggtg agaatttgac agtgaccact gctttcttat atccgtcacc
     3001 gagatctgac gtttcgatct ttgcttcttt gttgatgatc tccacatcat ctgctccgtt
     3061 ttttgcaaaa atctttgcct taaaagaacc gtcaaaagct tccggataga tcatttcata
     3121 gcttaaaaca ttataagccg atacatcgag tccttctgca aacgactttt taatctttgc
     3181 ctcactccat gtaaaacctt cacaaccggt ataatcgaga tctgccttta acattttgct
     3241 tccctgaaat tcagctacac tgattcctac cgcattgtcg tactgccagc cgccttcctg
     3301 ctcccagcct gcactgcttc ctgtctctgc agattcaaaa tcatcgatca cactcggatc
     3361 ttttgcaggc agttccggct gcgcttctgc cacaccgctt aatgtaacat tggaaaatgt
     3421 acatgttcct gaaaagccct gaccaatcag tcggaaatat acacctttta aatcatctgc
     3481 atccttatcc tcaaacttga tcgtaatcgt tgttttatgt gtatcttctg aaaaactgct
     3541 ctgctgtaaa tacgggatat cctgactgtc tgtccatgtc cactcacttc ccagcttcac
     3601 gataccctga acctttaagt aactttcccc ggtacccaat gatgcgtacg cctcatcatc
     3661 cagtgtaata tctgccttca ttgtgaaatc tttcgttgtc acacaatctg caatactttc
     3721 atcataagtg atcattggcg gattatccca atcctctgtt gcaagatcca gtttgtcttt
     3781 tgttactgtg tttgtcccgg tataaaacac actcgcttct gccgcataga catactcgta
     3841 tccccctgca aaattgccgc ttgaaaacgc aactgcagcc gctaaaagag cggctgcaag
     3901 tcgttttccc ataaaacgtt ttctcataaa cttacctcca atacatttat tagctttatg
     3961 cactttgtct tttaattata tgttacttta aaaaacattg tcaattaaga ttatattagt
     4021 ttgtaaattt tcagagtttt tacttaattt ttgctttttt aagttgtttt atgagattaa
     4081 aattgtatac ttttacttta taaattcaga taataaattt tactctaact caatccagtc
     4141 caacttcagt tccgatgtca catactctgc tttcagttca taataccccg gcataaattt
     4201 cacacgacac atccgctgtg tcaggaaacg ctctccggtt ccacctgtct ggacggttgc
     4261 catcagttct ccattcagat acagattggc agctccctgt gctaatcctt ccaccgggaa
     4321 acagaaatgt gcattaacgt ttaaaatgct ttctttttcc acataaagga ctgtgttttc
     4381 aaactttcct gcctcgatcc ggtttccgct ctgcagtgca cagacctgct tttcgtctgt
     4441 atttactgct tcttttgcag taactgtaag cagtggatca aaatctttta acggacgttt
     4501 catcacaggt gttccaagca ggaaacggca gatgtttttc gcacagcgct gtaactctcc
     4561 aacagtgagt tttcccgcct ccaacgcttc tacggtatca tcgcccgctg cgttgatggc
     4621 agcaccatta ttgttgacta ccatgtataa gtcattctgt gcacgaacca tgaatgaggt
     4681 gtatctgcgg tctgcctccc cgccgtttac aacgtcattc atctttgccc accagtctgt
     4741 cataacaatt ccctgatatc cccactcgcc acgcaggatc gtcgtattta agtcataatt
     4801 ggatgccgtc cagtgtccgt tgaccgggtt gtaagaggtc atgatcgaat ttgcatttcc
     4861 ctctttcacg gcaatctcaa agcctttcag atagatttca cggagtgccc gctcagatac
     4921 aacggagttt acattatgtc tttccgtctc ctgattattc gctgcaaaat gtttcacggt
     4981 cgcagaagaa ccggcacttc ggattcccct tgtcattgcc gccgcaaact gtcctgtcac
     5041 aagcggatct tcactgaaat actcaaagtt acgtccattt aatggatagc ggtggatatt
     5101 gattcccggt ccaagcaggg tgtcgatctc gtttccaact aattccctgc cttccatctg
     5161 atataattct tccatcattg gaatattaaa ggaacatgca aggagtgttc cgatcgggag
     5221 ctgtgttgcc tttaatccgc tctccatacg gatgccggac gggccatcgg atgcgcaggc
     5281 aatcgggatg ccataaccat gcaggctgtc gctgacaccg ccaaatgcgg atgctgtacc
     5341 cggtgttacc ttcgggctgc tcattccctc gccgcggacg atcacggcta actcctcgtt
     5401 ggtaaactgt gctacaaatg cgtccaattc ttcttccaca ctgctgccat ttttgatatt
     5461 ctctgcaacc gcctgtaagg taattccttt atttccggtc tgtggcagtt cttttggcag
     5521 acggcttttg atccgctctg ccagatcaac catctgctgt ggtgcttttt catatgcacg
     5581 ggcaaacacc ccgtcctcct tttgtctgcc tgtcttaaga cgcaaaaatt cttttgtcgg
     5641 cgcaagcgcc tcctccaact cctctgtgac gatacaggaa gaaacctcat aaccgccttt
     5701 tccgtccaca ttggcttttt ctgtgcatct gacgctgttt ccaacataga tcttatacag
     5761 accttcctct aagacatagc aggacttatg accagtcaca ccgctgtcgt cataggaagc
     5821 aaaccggctt accggaatgg taagttcaag tacttcctcc tcgcccggag caagcagttt
     5881 tgtctttgca aatccgcaca atacccttgc cggttttcca agctgtccct gtggggcgga
     5941 cacataaacc tgtgcagcct cttttccggc tgctgccccg gtatttttca cacggatact
     6001 gagtgcgatc tttgcatctt ttccatttcc tgtactctcc gcttttacca tctccatgga
     6061 aaattccgta taagataaac cgaaaccaaa ctcatacatt actttttccg gtgcgaatgt
     6121 ctcaaaataa cggtagccga catagatatc ttccgcataa atattttctg tcttacttcc
     6181 aaaatggtct gctgccgggt aatcagaaag tttctccgcg atcgtatccg tcagttttcc
     6241 ggatggtgtc actctgcccg ataacagatc tgcaacggcg ttcgcgcctt ccataccacc
     6301 ctgccagatg taaagaatcg ctttgatgtg atcctgatat gccgcatctt tcgtccagct
     6361 catatcaatg atgttagaca catttaaaag cactgccact tcgttaaaat attttgttac
     6421 aacacgaaga ttttctttct cccgttttgt cagaagataa ctgccttccg tgtcctcgta
     6481 atctttatcc tcaccggcag ttcttccgat cagaaaaatt gctttttcag acttttctgc
     6541 tgcgcggcgt gcgatctcgt cggtgatctc catctcttcc tgatgccacg gctctgccgc
     6601 ccatccaccg cctccattgt caaacggatg ctcctttaac cattctttat aggtttctac
     6661 cagatcctca tttacaggaa aagcattatt ttctttgatt ccgtctaaaa tatttttgac
     6721 atacggcaca ttgaccgctc cacctgaacc ggttccgctc cggtaatatt cgatctggct
     6781 tcttccgaaa atagaaactg tctcatcctt tttgatcgga agcatatgcc cctcattctt
     6841 taaaagcacg gctccctctg ccgctgcctc tctgcagtat gctgccagtt ccggcagcgg
     6901 aacaccgatt ccactgttac ccatgataat cctccatttt aatctccatt cttgcgctgt
     6961 ctttcaatga aaacaacgtt ttcatttgca tctcaagttt gtgcgtaagc acaaatcttg
     7021 cgtgtgaaaa atcttatttt tcacactata tatctgttgt tatcttttta gcctgtcatt
     7081 atttttgtct atgattgttt ttcgtctgat ctcagtcgat tttcagtatt aatcctaaaa
     7141 tacgctttgc gcagatctcc atctcttctc ttgtcagcac tcctgcttct tttgcacgga
     7201 gcagactctc cggaaatcca catgccatct tgatgtcatt tcccgctttt acttctttat
     7261 aatgctcacc gcttgtccac cagtcggtcg tgaccattcc ctgataattc cactcacctc
     7321 ttaagatgtc ctctaaaagt tcacggtttt cagaagttct gtgtccgttt acgatattgt
     7381 aagatgacat aatgcaccac ggatctgcct cttttacgat aatctcaaac gcctttaaat
     7441 aaatctccct tgctgcccgc tcggacactc tggaatcgct ttccttacgg tttgtctcct
     7501 tattgttgca tgcaaaatgt ttgaccgtcg cggcaatatg ttttgactgg attcccttta
     7561 ccattgcaga tgccattttc ccggtcagat acgggtcttc ggaataatac tcgaagtttc
     7621 ttccgcaaag cggacttctg tggatattga ccgcaggcgt cagccagacg gcaatgttat
     7681 tttccttcac ctcagcggca cctgctgcac caacctgata taacatatcc gggttccagg
     7741 tacatgctaa aagtgtcgca cacggccacg ccgtcgtaac tacgccgcat tttttctcaa
     7801 tgcggagtcc ggcaggtccg tccgccgtca taatattcgg gattccatac tccggcatat
     7861 ttccaaatcc aaatgtattt gccacaccgg tattcggctg tccgcctaaa atatgcgcaa
     7921 gttcctcatc cgtcatctgt gtcacaaatt catccagcgt gatctttcct tctgcaactt
     7981 ccataaaaat atgtgcgtct ttcttatacg gctgtgacca gatctgatgt cccggcagtg
     8041 cgcggacagc cggtgaaaat ccatcgcttt cctccctcgg aacacgtttg attgcggagt
     8101 aatccggatc attcggttcg gactgcggca gtttttcaaa acttccatcg gagagcatgc
     8161 gctccgcaag taaagtcgga accagttttg cggtaagctg ctctgttacg gtatcctctg
     8221 gcatcgtaaa acaaaatacc tgctctgttt cccgcacgga cgtgccaaga taaaactgat
     8281 acttaccttt ttccagcaca taggcagatt tctgaatttt tcccagatca tcataggatg
     8341 caagctgtgc caccggtgcc tctaacgtca gaagctgcgt ttctccctgt gctaacagtc
     8401 gtgttttttc aaatgctgca aggactctct ttgcctttcc aagtacgccc tgcggtgcct
     8461 gcgcatataa ctgcatgact tcttttcccg gatattttcc ggtgttggtc acattgacac
     8521 gcacgcggat cacaccctgt ttttcttccg cagatactaa cgcacgctcg aaagtcgtgt
     8581 aggaaagacc gtagccaaac ggataattga cggctttatc agcccccggt attgtctcaa
     8641 aatagcggta gccgacgtag atatcttctt tataatcgac atattttacc gattcatgga
     8701 atgtctccga tgacggatag tcttccagtt tctttgcaaa tgtgtcggaa agttttccgg
     8761 atggactgcc gacgccgcac agaagctctg ctgctgcgct tccgccttcc atacccccct
     8821 gccatgccat cagcaccgcc tgaatttttt cattttctgc aaaccagtcc gtatcgacca
     8881 tgccgccgac attcataacg actgccacct tcgggaacaa ctgctgcacc tgttccacca
     8941 tcgcgctctc ttccttcgtc aggtaaaagt ctccgtccgg aaaaatgcgc tctgcctttt
     9001 ccagcagcgg atctgtctgt accgactcat ccatctcctt atcataagaa ctctttctgt
     9061 cccagccttc cccggaaaaa cggctgatgc agatcactgc cgtgtccgtg tacgctctcg
     9121 ctttttttgc agtttcttcc ggaaacgcag gctctactgt cattcccggt tctgctcctg
     9181 cctcgtactg tttttctacc tctttacggt aataatcaga gagtgcctca tacacttcta
     9241 ctttttccgg cagcttcttg aatccctcat acaaatttgt cgtataagca acggttacat
     9301 ctccgcttcc gccaccaccc ttgacatagt caaaactgcc ttttccaaac agggcgagtt
     9361 tggtgcctgc ctgaaatggg agaaggcttt cattgttctt tagaagaacc atgccttctt
     9421 tggcggcttc ttttgacagg gtgatgtgtg cttttgaggc ggtgactttg gtgccgtcag
     9481 ctccgagagg gaggttgggt tgataaagtg atctttgcca tttttccat
//
