LOCUS       ACXX02000019            6138 bp    DNA     linear   BCT 14-MAR-2011
DEFINITION  Ruminiclostridium papyrosolvens DSM 2782 ctg47, whole genome shotgun
            sequence.
ACCESSION   ACXX02000019
VERSION     ACXX02000019.1
DBLINK      BioProject: PRJNA33587
            BioSample: SAMN00002727
KEYWORDS    WGS; GSC:MIGS:2.1; NONCONTIGUOUS_FINISHED.
SOURCE      Ruminiclostridium papyrosolvens DSM 2782
  ORGANISM  Ruminiclostridium papyrosolvens DSM 2782
            Bacteria; Firmicutes; Clostridia; Clostridiales;
            Hungateiclostridiaceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     The Non-contiguous Finished genome of Clostridium papyrosolvens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L.,
            Mouttaki,H., He,Z., Zhou,J. and Hemme,C.L.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 6138)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name             :: Clostridium papyrosolvens DSM 2782
            collection_date          :: Missing
            lat_lon                  :: 57.162143 -2.719417
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: United Kingdom
            environment              :: Mud
            num_replicons            :: Missing
            ref_biomaterial          :: DSM 2782, ATCC 35413, NCIB 11394
            biotic_relationship      :: Free living
            trophic_level            :: Chemoorganotroph
            rel_to_oxygen            :: Anaerobe
            isol_growth_condt        :: Missing
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            GOLD Stamp ID            :: Gi02584
            Type Strain              :: Yes
            Greengenes ID            :: 15957
            Isolation Site           :: estuarine sediment, River Don,
            Source of Isolate        :: Christopher L. Hemme (hemmecl@ou.edu)
            Cell Shape               :: Rod-shaped
            Motility                 :: Motile
            Sporulation              :: Sporulating
            Temperature Range        :: Mesophile
            Temperature Optimum      :: 25C
            Gram Staining            :: Gram-
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Noncontiguous Finished
            Current Finishing Status :: Noncontiguous Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454; Illumina
            ##Genome-Assembly-Data-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085558
            Source DNA and Organism available from Christopher L. Hemme
            (hemmecl@ou.edu)
            Contacts: Christopher L. Hemme (hemmecl@ou.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            Aberdeenshire, Scotland
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Ruminiclostridium papyrosolvens DSM 2782"
                     /mol_type="genomic DNA"
                     /submitter_seqid="ctg47"
                     /strain="DSM 2782"
                     /isolation_source="estuarine sediment from River Don"
                     /type_material="type strain of Ruminiclostridium
                     papyrosolvens"
                     /db_xref="taxon:588581"
                     /country="United Kingdom: Aberdeenshire, Scotland"
     gene            1..1698
                     /locus_tag="Cpap_0272"
     CDS             1..1698
                     /locus_tag="Cpap_0272"
                     /inference="protein motif:PFAM:PF00404"
                     /note="PFAM: Dockerin type 1; KEGG: cce:Ccel_0752 glycoside
                     hydrolase family 26"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM35|GH26"
                     /protein_id="EGD45902.1"
                     /db_xref="GO:0016787"
                     /db_xref="InterPro:IPR000805"
                     /db_xref="InterPro:IPR002105"
                     /db_xref="InterPro:IPR018242"
                     /db_xref="InterPro:IPR018247"
                     /translation="MALTALMPLVSVVNVPANAMTAEGVKYEFENGVQNNAEIHTDYVG
                     KTDDGLIFDLSGATCSYVGQRGTSTSVNVDIEQAGLYELVVRYAQPFDKTKKIQYLNVN
                     DSNQGEVSFPYTLNWKEMSAGIVKLNKGTNNIEFEGYWGYTFFDYLIVKPADESITSLK
                     VDKKLVNPNATKETKALMSYLVDVYGKKIISGQQEICGSHNYEGSESDFAYIKEKTGKM
                     PALRGFDFMNFRGNGLLWDDLCAERVIDWYKNKGGIPTVCWHWFSPGNIGKKADNSFYT
                     ESTTFSISKALTSGTAENTALLNDIEFMAGKFKQLQEADVPVLFRPLHEAEGGWFWWGA
                     EGPENCVKLYRLLYEKFTNEYHLNNIIWVWTSYTYDTSSKWYPGDDVVDILGYDKYNAK
                     DGLPNGSAISSTFYNMIKLTNGKKLVTMSENDTIPQVSNLTNEMAGWLYFCPWYGWYLT
                     GEQNNPVDWLKQMYTSDYCITLDELPDLETYPFPSGGDTPTITYGDLNGDNLIDAIDLA
                     LLKKCILDNNQSIKSADVDGNGSVDAIDFVYMKKFLTGEISSFPASGK"
     gene            1734..4070
                     /locus_tag="Cpap_0273"
     CDS             1734..4070
                     /locus_tag="Cpap_0273"
                     /inference="protein motif:PFAM:PF00759"
                     /note="PFAM: glycoside hydrolase family 9; Dockerin type 1;
                     KEGG: cce:Ccel_0753 glycoside hydrolase family 9"
                     /codon_start=1
                     /transl_table=11
                     /product="GH9|CBM3"
                     /protein_id="EGD45903.1"
                     /db_xref="GO:0016853"
                     /db_xref="GO:0030246"
                     /db_xref="InterPro:IPR001701"
                     /db_xref="InterPro:IPR001956"
                     /db_xref="InterPro:IPR002105"
                     /db_xref="InterPro:IPR018242"
                     /translation="MKKGLIFKVARASVMSVVVLAMLLTSFTVMNVSAASTDYNYAKLL
                     QYSQYFYDANMCGTEVSEKNQYTWRNDCHTYDAKLPLDSKNTNMTDSFISQNKSVLDPD
                     GDGTVDVSGGFHDAGDHVKFGMPEAYSGSTLGWGFYEFKDQYVTTGQDSHIKTILRYFN
                     DYFMRSTFLDSTGKAIAFCYQVGDGDVDHAYWQAPEVDEMFRRGWFATDEMPSTDCVAA
                     AAASLAVNYMNFKDEDPAYAEKSLKYAKALFQFAERCPSKSVNIDGPKGYYGSSKWQDD
                     YCWAASWLYLATKDDHYLDETLKNFDYYAPSCWTHCWNDVWAGTACIIAEINDSIDKNG
                     QVLEDRYKKLTNKSSYEEIDFWSQIAKLADGWMSGKNVTVSPGGYAFLNQWGSARYNTA
                     TQLLALVYDKNHGDKASKYSQWAKSQMDYLLGNNPLNRCYVVGYGDNSVKYPHHRASSG
                     YATAEGTGPQKYVLYGALVGGPDASDQHKDITSDYVYNEVTIDYNAAFVGASAGIYRYF
                     GNSAMQVTPNFPPPPKEDDPDDPSGSKYWVEGFCVDVAQSDGPKATEVTLYVKSDVSKT
                     SKNISVRYYFDSTGMTSLDPNKIEMRQLYDQTAAETDYAAEISGPHLYKDKIYYIEVKW
                     PGYVIANSNKKYQFAIGTYAWQNNWNPTDDWSHKGLKVVEDSWKGTPEKTDNICVYDNG
                     VLVGGTEPDGTTPGGNNPEPTIKLGDINNDKSIDSLDLTLMKMHILGVKILEGNELKAA
                     DMDASGDVDSIDYAKLKKIILTQAD"
     sig_peptide     1734..1838
                     /locus_tag="Cpap_0273"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.958) with cleavage site probability 0.895 at
                     residue 35"
     gene            4114..6138
                     /locus_tag="Cpap_0274"
     CDS             4114..6138
                     /locus_tag="Cpap_0274"
                     /inference="protein motif:PFAM:PF00759"
                     /note="PFAM: glycoside hydrolase family 9; Dockerin type 1;
                     KEGG: cce:Ccel_0755 glycoside hydrolase family 9"
                     /codon_start=1
                     /transl_table=11
                     /product="GH9"
                     /protein_id="EGD45904.1"
                     /db_xref="GO:0016787"
                     /db_xref="InterPro:IPR001701"
                     /db_xref="InterPro:IPR018221"
                     /db_xref="InterPro:IPR018242"
                     /db_xref="InterPro:IPR018247"
                     /translation="MGTPFAFTKLIAVLLQGGNNSTKILEVDCMSLISYERRRKLRAGL
                     ACFVVSGIVATSGGLIPGTFSQNVSAASTSPGDYQQDSRIRLNSIGYLPGAEKKATIAA
                     SASDFILVNSSGTAVLTGKTTSTNNSDTNEQVSIADFSSIKTNGTYTLLVPGIGKSVTF
                     KIDPNIYVNPFKTSMLGMYLWRCGTSVSTTYNGKVFSHEACHTKDGYTDYINGQHNIKD
                     GGKGWHDAGDYNKYVVNAGITVGSMFFAWEQFKDQIKGISLTMPESNNSMPDYLDELKY
                     ETDWLLTMQYPDGSGKVSHKLTTKDFGGFVLPERETADRFFTPWGSAATADFAAMMAMA
                     SRAFRPYDASYADKCIAAAKVSYACLKANPGNTKPNLSGFTTGDYDTTDTDDRLWAAAE
                     MWETTGDNSYLADFESGANYFSKKIDVDFDWGNVNNLGMFTYLLSEKSGKNASLYNTIK
                     NALISAADSIVGTADKHGYARPLGETYYWGCNGTVARQTMILNIANKLSPKADYVNTSL
                     DALNFLFGRNYYNRSFVTGMGINPPMKPHDRRSGGDSQIDPWPGYLVGGGWPGAKDWKD
                     DQDSYQTNEIAINWNGALIYALAASLDVSDTSDLLLGDINMDANVDSIDYALMKQHILG
                     LSILSGDAFKAADVNHDNAVDAIDLSTLKQYLLGKITSL"
ORIGIN
        1 ttggcattga cagcactgat gccattagtg tcagttgtaa atgttcctgc aaatgccatg
       61 acagcagaag gtgtaaagta tgaatttgaa aacggtgtcc agaacaatgc tgaaattcat
      121 actgactatg ttgggaagac cgatgacggc ctgatatttg acctaagcgg tgcaacttgc
      181 agctacgttg gacaaagggg gacaagtact tctgttaatg ttgacattga acaggcagga
      241 ctttacgaat tggttgtacg atatgcacag ccatttgata agaccaaaaa gatacagtat
      301 ctcaatgtca atgattccaa tcaaggagag gttagctttc cgtacactct caactggaag
      361 gaaatgtccg caggaattgt aaagctcaat aagggtacta acaatattga atttgaaggc
      421 tactggggat acactttttt tgactacctt attgttaagc ccgcagatga aagtattact
      481 tcactgaaag tcgataaaaa gcttgtgaac cctaatgcta ccaaagaaac aaaagcactt
      541 atgagctact tggttgacgt gtatggaaag aaaattattt caggtcagca ggaaatttgc
      601 gggtctcata actatgaagg ctcagagtca gactttgcct atattaagga aaaaacaggc
      661 aagatgcctg cattaagagg ttttgatttt atgaacttca gaggaaacgg cttgttatgg
      721 gatgatcttt gtgcggaacg tgtcattgac tggtacaaaa ataaaggtgg tataccaaca
      781 gtttgctggc attggttttc gcccggaaat atcggcaaaa aagctgataa cagcttctat
      841 acagaaagca caaccttcag tatatcaaag gctttgacat ccggaactgc cgaaaacacc
      901 gcactgctta atgatattga atttatggca ggaaaattca aacagctaca ggaggcagat
      961 gttcctgtac ttttcaggcc actccatgaa gcagaaggag gatggttctg gtggggggcc
     1021 gaaggccctg agaattgtgt aaagctatat aggctgttat acgagaaatt tacaaatgaa
     1081 taccacttaa acaatatcat atgggtatgg acttcctata catatgacac atcttcaaaa
     1141 tggtatcccg gtgatgatgt tgttgatatt cttggctatg acaaatacaa tgccaaggat
     1201 ggactgccaa acggaagtgc tatttcttca accttctata atatgattaa actcacaaac
     1261 ggtaagaagc ttgttacaat gagtgaaaac gatacaattc cgcaggtttc aaaccttaca
     1321 aatgagatgg ccggatggct atatttctgc ccttggtacg gatggtatct tacgggagag
     1381 cagaataatc cggttgattg gctaaaacag atgtatacca gcgattattg tattacactg
     1441 gacgaacttc ccgatcttga aacctaccca ttcccatctg gcggggatac tcctacaatc
     1501 acgtacggtg atttgaatgg agataaccta atcgatgcaa ttgatctcgc tttactaaaa
     1561 aaatgtatat tagacaacaa tcagagtata aagtcagcag atgttgacgg taacggttct
     1621 gtagacgcta tagatttcgt atatatgaag aaatttctga caggtgaaat ttccagcttt
     1681 ccagccagtg gaaagtaatt gatttttttt atttaaagaa aggaagatat attatgaaaa
     1741 aaggattgat ttttaaggta gcaagagctt ctgtaatgag cgttgttgtc cttgcaatgc
     1801 tgctaacttc attcactgtt atgaatgtaa gtgcagcaag tacagactat aattatgcta
     1861 aactactaca gtactcacag tatttttacg atgcaaatat gtgtggtacc gaagttagcg
     1921 aaaaaaacca atacacttgg agaaatgact gtcatactta tgatgcaaaa ctaccgctag
     1981 attcaaaaaa tacaaatatg acagatagct ttatcagcca aaataagagt gtgctagatc
     2041 ctgatggaga cggaacggta gatgtttccg gtggttttca tgatgcaggt gaccacgtaa
     2101 aattcggtat gcctgaagcg tactcaggtt caactcttgg atggggattt tatgaattca
     2161 aagatcaata tgttacaacc ggacaagaca gtcatataaa aacaatatta agatatttca
     2221 atgattactt tatgagaagt actttccttg actcaaccgg taaggctata gcattctgct
     2281 atcaggttgg agacggagat gtagaccacg catactggca agcacctgaa gttgatgaaa
     2341 tgttcagaag aggctggttt gcaactgatg aaatgccttc aactgattgt gtggcagcag
     2401 cagcagcttc acttgcagtc aattacatga attttaagga cgaggaccct gcatatgcag
     2461 aaaaatcact taaatatgca aaagccttat ttcaatttgc agaaagatgt cctagcaaat
     2521 cagtaaacat tgatggacct aaaggttatt acggatcctc caaatggcag gatgattact
     2581 gctgggctgc atcatggctg tatttagcaa caaaggatga ccattacctt gacgaaacac
     2641 taaagaattt tgattactat gcaccttcat gctggacaca ttgctggaac gatgtttggg
     2701 caggtacagc atgtattatt gctgaaataa atgattcaat tgataaaaac ggacaagtat
     2761 tggaagatag atacaaaaaa ttaacaaata aaagttcata tgaagagatt gatttctgga
     2821 gtcagattgc aaagttagca gatggctgga tgtcgggtaa aaatgttaca gtatcacctg
     2881 gcggttacgc ttttctgaat caatggggtt ctgcgagata caatacagct actcagctgt
     2941 tagcacttgt ttatgataag aatcacgggg ataaagcatc aaagtacagc caatgggcta
     3001 aatcacagat ggattacctt ttaggtaaca atccgttaaa ccgctgctac gtagtgggat
     3061 atggagacaa ttcagtaaaa tatcctcatc acagagcttc atcagggtat gctactgcag
     3121 aaggaacagg tcctcaaaaa tatgttcttt acggtgcatt agttggtgga cctgatgcca
     3181 gcgaccaaca taaggatatt acttcagact acgtgtataa tgaagtaaca atagactaca
     3241 acgctgcatt tgttggagct agtgccggta tttacagata ctttggaaac tcagctatgc
     3301 aggttacccc taacttccca ccacctccaa aagaagacga tcctgatgac ccttccggtt
     3361 caaaatattg ggtagaaggt ttctgtgttg atgtagctca atcagacgga ccaaaagcaa
     3421 ccgaagttac actgtatgta aaatctgatg tttcaaaaac ctcgaaaaat atttctgtaa
     3481 gatattattt tgattctaca ggaatgacat cccttgaccc aaataaaata gaaatgagac
     3541 agttgtatga ccaaacagct gctgaaacag attatgcagc tgagatatca gggccacacc
     3601 tctataaaga taaaatctat tatatagaag taaaatggcc gggttatgta attgcaaatt
     3661 ccaacaagaa ataccagttt gcaataggta catacgcttg gcaaaataac tggaatccta
     3721 ctgatgactg gagtcataag ggattgaagg ttgttgagga cagctggaaa ggaacacctg
     3781 aaaagacaga caatatctgt gtttatgaca atggcgttct agttggcggt acagagcctg
     3841 atggaactac accgggcgga aataatcctg aaccgacaat taaattgggt gacataaaca
     3901 acgataaatc aattgattcg ttggacttaa cattgatgaa aatgcacata ttaggtgtca
     3961 aaattcttga aggaaatgag cttaaggcag ctgatatgga tgccagcggc gatgtagatt
     4021 ctattgatta tgctaagttg aagaaaatta tattgacaca ggcagactaa taaaaaattg
     4081 caaagggagt ttatataact accaggagtt ggcttgggaa ctccctttgc ctttactaaa
     4141 ttaatagctg ttttacttca aggaggtaac aacagtacaa aaattttgga ggtagattgt
     4201 atgagtttaa taagttatga aagaaggcgg aaattaaggg cagggctagc atgttttgtt
     4261 gtaagcggaa ttgtggcaac atccggaggt ctcattcccg gaacttttag tcaaaatgta
     4321 tctgcggcaa gtacatctcc cggagactac cagcaagatt cccgtatacg tctcaattct
     4381 atagggtatc ttcccggagc tgaaaaaaaa gcaacaattg cagcctctgc aagtgatttt
     4441 attttagtaa attcaagtgg aacagctgtt ctcactggta aaactacttc caccaacaac
     4501 tctgatacca atgagcaggt tagcatagca gatttttcgt caataaagac gaatggaact
     4561 tacaccctgc ttgtacctgg tataggaaaa agtgtaacct tcaaaattga cccaaatatt
     4621 tacgtaaacc catttaaaac atcaatgctt ggaatgtatc tgtggcgctg cggaacatca
     4681 gtttccacaa cctataatgg caaggtgttc tctcatgagg cttgtcatac caaagatgga
     4741 tatactgact atattaatgg acagcacaat ataaaagatg gcggaaaagg ctggcacgat
     4801 gcgggggatt acaacaaata tgttgttaat gccggtatta ccgtaggctc tatgtttttt
     4861 gcatgggagc agtttaaaga tcagataaaa ggcatttcct taacaatgcc tgaaagcaac
     4921 aactcaatgc cggattattt ggatgaactc aaatatgaaa cagattggct tttaaccatg
     4981 caatatcctg acggaagcgg aaaggtaagc cataagctta ctacaaaaga ctttggtgga
     5041 tttgtgttac ctgagagaga aactgccgac aggtttttca ctccatgggg aagtgctgca
     5101 acagcagact ttgcagctat gatggctatg gcttcaagag ctttccgtcc atacgatgcg
     5161 tcatatgctg acaaatgtat tgctgctgca aaagttagtt atgcttgttt aaaggcaaat
     5221 ccgggaaata cgaagcctaa tctcagcggt ttcactacag gtgattatga tactacagac
     5281 actgatgata gattgtgggc tgctgcagaa atgtgggaaa ctaccggaga caacagttat
     5341 ctggcagatt ttgaatccgg tgcaaactac ttttctaaga aaattgacgt tgacttcgac
     5401 tggggaaatg taaacaatct tggaatgttt acatacctgt tatccgaaaa gagcggaaaa
     5461 aacgcttcac tatataacac aattaagaat gcattaattt cagcagcaga cagtatagtt
     5521 ggtactgctg acaaacacgg ttatgcaagg ccactgggag aaacctatta ctggggctgc
     5581 aacggtaccg tggcacgtca gaccatgatt cttaatattg caaataaatt atcgccaaaa
     5641 gcagattatg ttaacacttc gctggatgca ctgaactttc tgttcggcag aaactattat
     5701 aatcgttcct ttgttacagg aatgggaata aaccctccaa tgaaaccaca tgacagacgt
     5761 tcaggcggtg attcccaaat tgatccatgg cccggatatt tagttggtgg tggttggccg
     5821 ggagcaaagg attggaagga tgatcaggat agttatcaga ccaatgagat tgcaatcaat
     5881 tggaatggtg cattaattta tgcactggcc gcatcacttg acgtatcgga tacttccgac
     5941 ctactattgg gagatataaa tatggatgca aacgttgatt ccatcgatta cgctctgatg
     6001 aaacagcata tattgggttt gagtattttg tctggagatg catttaaagc tgctgacgta
     6061 aatcatgaca atgcagtaga tgctatagat ttatcaactt taaaacagta tttgttaggt
     6121 aagattacaa gcttataa
//
