LOCUS       SIWZ01000001            6515 bp    DNA     linear   BCT 29-JAN-2020
DEFINITION  Bacillus subtilis strain ABP1 contig1_ABP1, whole genome shotgun
            sequence.
ACCESSION   SIWZ01000001
VERSION     SIWZ01000001.1
DBLINK      BioProject: PRJNA523170
            BioSample: SAMN10965541
KEYWORDS    WGS.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Serra,C.R., Alburquerque,P., Matas,I.M., Munoz-Merida,A.,
            Oliva-Teles,A., Enes,P. and Tavares,F.
  TITLE     enome sequence of 2 marine fish-gut Bacillus isolates with
            NSP-hydrolytic potential
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Serra,C.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2019) Biology, Aquaculture and Seafood Quality,
            CIIMAR - Interdisciplinary Centre of Marine and Environmental
            Research, Erminal de Cruzeiros de Leixoes. Av. General Norton de
            Matos s/n, Matosinhos, Porto 4450-208, Portugal
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date          :: 13-FEB-2015
            Assembly Method        :: Pacific Biosciences SMRT Analysis v.
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 258.0x
            Sequencing Technology  :: PacBio RSII
            ##Genome-Assembly-Data-END##
            Bacteria and source DNA available from NUTRIMU collection.
            2.3.0
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Bacillus subtilis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig1_ABP1"
                     /strain="ABP1"
                     /isolation_source="gut"
                     /host="Dicentrarchus labrax (European sea bass)"
                     /db_xref="taxon:1423"
                     /country="Portugal: Porto"
                     /collection_date="10-Sep-2013"
                     /collected_by="Claudia Serra"
                     /identified_by="Claudia Serra"
     gene            complement(1..900)
                     /gene="ytaP"
                     /locus_tag="ABP1_4096"
     CDS             complement(1..900)
                     /gene="ytaP"
                     /locus_tag="ABP1_4096"
                     /EC_number="3.-.-.-"
                     /note="GO_function: GO:0004252 - serine-type endopeptidase
                     activity; GO_function: GO:0016787 - hydrolase activity;
                     GO_function: GO:0008236 - serine-type peptidase activity"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydrolase YtaP"
                     /protein_id="KAF1343299.1"
                     /translation="MRVERRKQLFRLLGDLPDRRPISVETLRIEEREENIVETLLLDLN
                     GHEKVPAYFVKPKKTEGPCPAVLFQHSHGGQYDRGKSELIEGADYLKTPSFSDELTSLG
                     YGVLAIDHWGFGDRRGKAESEIFKEMLLTGKVMWGMMIYDSLSALDYMQSRSDVQPDRI
                     GTIGMSMGGLMAWWTAALDDRIKVCVDLCSQVDHHVLIKTQNLDRHGFYYYVPSLAKHF
                     SASEIQGLIAPRPHLSLVGVHDRLTPAEGVDKIEKELTAVYAGQGASDCYRVVRSASGH
                     FETAVMRHEAVRFLQKWL"
     gene            1118..2152
                     /gene="msmR"
                     /locus_tag="ABP1_4097"
     CDS             1118..2152
                     /gene="msmR"
                     /locus_tag="ABP1_4097"
                     /EC_number="5.1.1.1"
                     /note="GO_function: GO:0003700 - transcription factor
                     activity, sequence-specific DNA binding; GO_function:
                     GO:0008784 - alanine racemase activity; GO_function:
                     GO:0003677 - DNA binding; GO_process: GO:0006351 -
                     transcription, DNA-templated"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558"
                     /protein_id="KAF1343300.1"
                     /translation="MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEEL
                     GYQTVAKRRKSRGQKQRAQPLIGVLSCLSPDQERQDPYFSSIRKGIEKECFEQEIFITN
                     SIHLGSFQEHIFRELDGVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGIDFE
                     SASRQAIDHLLDLGYKRLGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHV
                     LIGEYSMREGYRLMKKAIDQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFN
                     GIEEAEFASTPLTTVKVYTEEMGRTGVKLLLDRLNGRTLPHHVTLPTTLIVRQSCGCTA
                     KEVT"
     gene            2186..3466
                     /gene="msmE"
                     /locus_tag="ABP1_4098"
     CDS             2186..3466
                     /gene="msmE"
                     /locus_tag="ABP1_4098"
                     /note="GO_function: GO:0005215 - transporter activity;
                     GO_process: GO:0008643 - carbohydrate transport"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="KAF1343301.1"
                     /translation="MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSER
                     DTFRKLAAAFEKEHSDIHVSLHFPGNDYENMMRVRMAANDLPDLFDTHGWGKIRYGEYT
                     ADLRDMEWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIAPPETMDD
                     FIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDLAHNEKKQLINGTFQ
                     WSKFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQINP
                     NVKVGIIPTPAIHPGDNPIWIGGERYTLAAWKDSPQLKEAKEFIAFMARPANAKQMAEA
                     TSLPSGLTNVKADIFYANDYEHYQDVKVEPYFDRLYLPNGMWDVLGTVGQELAADIMAP
                     QEISQKLGREYKRLREQSETQGAENNE"
     gene            3459..4370
                     /gene="amyD"
                     /locus_tag="ABP1_4099"
     CDS             3459..4370
                     /gene="amyD"
                     /locus_tag="ABP1_4099"
                     /note="GO_component: GO:0005886 - plasma membrane;
                     GO_component: GO:0016021 - integral component of membrane;
                     GO_process: GO:0008643 - carbohydrate transport;
                     GO_process: GO:0006810 - transport"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O51924|3.A.1.1.7"
                     /protein_id="KAF1343302.1"
                     /translation="MSEIARDVHVKQVKPKKQSSLWWMYIPALLSVLVFMIYPFVKGTL
                     ITFTNWNGFSQVYQWVGFAQYERLFSDPDTWHILKNTLLYGLGSTFFQNVVGLLYALLL
                     NQSIKTKAVTRTIVYLPVMISPLIMGYIWYFFFSYDGGALNDLLGVFGISPINALASPS
                     LNPWIIVMINTYQYVGIAMVVYLAGLQSIPKDYYEAAQMDGAKQGQQFFTITLPLLMPS
                     ITINMVINIIGGLKLFDVIIALTAGGPGNASQSMSTFMYDLYFKRQDAGYAATQGIFMA
                     FVILIISFCALAYFKRKETEMS"
     gene            4367..5197
                     /gene="amyC"
                     /locus_tag="ABP1_4100"
     CDS             4367..5197
                     /gene="amyC"
                     /locus_tag="ABP1_4100"
                     /note="GO_function: GO:0022891 - substrate-specific
                     transmembrane transporter activity; GO_process: GO:0006810
                     - transport; GO_process: GO:0008643 - carbohydrate
                     transport"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8RJU8|3.A.1.1.18"
                     /protein_id="KAF1343303.1"
                     /translation="MRAARTKSMRIITLLAAIVACAHFIPFYILLTTSLKAKGDYSSKW
                     IFPADISFHNFSEAWERASLGNSFINTMIITGFSALLLIIFGSLAAYPLARRETKLNKA
                     VFALLISIMIIPPLTSMVPLYRMVVDAGMVNTHAIAIFINTAAYMPLTVFLYSGFIRST
                     IPKELEEAARIDGAGMLKIFFTIVFPLLKPITATICIISCVFIWNDYQFAIFFLQDQKV
                     QTLTVAMAGFFGENANNLHLVAAAALMAMLPMVVLFLALQKYFIAGLSSGAVKG"
     gene            5217..6515
                     /gene="melA"
                     /locus_tag="ABP1_4101"
     CDS             5217..6515
                     /gene="melA"
                     /locus_tag="ABP1_4101"
                     /EC_number="3.2.1.86"
                     /EC_number="1.1.1.-"
                     /EC_number="3.2.1.22"
                     /EC_number="3.2.1.-"
                     /note="GO_function: GO:0052692 - raffinose
                     alpha-galactosidase activity; GO_function: GO:0046872 -
                     metal ion binding; GO_function: GO:0004553 - hydrolase
                     activity, hydrolyzing O-glycosyl compounds; GO_function:
                     GO:0004557 - alpha-galactosidase activity; GO_function:
                     GO:0008706 - 6-phospho-beta-glucosidase activity;
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor;
                     GO_process: GO:0005975 - carbohydrate metabolic process;
                     GO_process: GO:0005995 - melibiose catabolic process"
                     /codon_start=1
                     /transl_table=11
                     /product="GH4"
                     /protein_id="KAF1343304.1"
                     /translation="MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQE
                     SQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGL
                     RQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRYTNI
                     KTIGLCHSVQVCTKDLCKALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAK
                     EKQKTKHHDMVRFELMDKFGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRR
                     CVKQIENWEKMRDDIVNNKDLTHERSKEYGSRIIEAMETNEPFTFGGNVLNTGLITNLP
                     SKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTQKKEAVYQAAM
                     LDPHTSAELSMKDIISMCDDLFAAHGDWLPEYK"
ORIGIN
        1 tcacagccac ttttgcaaaa accggacagc ttcatgcctc ataactgctg tttcgaaatg
       61 tcccgaagca gaacggacca ctcggtagca atcagaagct ccctgtccag catagacagc
      121 tgtcaattct ttttcgattt tgtccacgcc ctcggccggc gttaaccgat cgtgtacacc
      181 aacaagacta aggtgcgggc gcggcgcgat caggccttgg atctccgatg cggaaaaatg
      241 ctttgcaagc gatgggacgt aataataaaa gccgtgccta tcaagattct gtgttttgat
      301 cagcacgtga tgatccacct ggctgcacaa atccacacac accttaatcc ggtcatcgag
      361 cgccgctgtc caccacgcca tcaaacctcc catagacatg ccgatggttc cgatcctgtc
      421 aggctgaaca tcagagcgtg actgcatata gtccaacgca cttaaactgt cataaatcat
      481 catcccccac atcacttttc cggtgagaag catttcttta aatatctcgc tttccgcttt
      541 tccccgccga tctccaaatc cccaatgatc aatcgcaaga acgccgtatc cgagtgaagt
      601 aagctcatca gaaaaagaag gcgttttcaa ataatccgct ccctcaatca gctcactttt
      661 tcccctatca tactggcccc catgcgaatg ctgaaacaat acggctgggc acggtccttc
      721 tgttttcttc ggcttcacaa aataagcggg caccttttca tgtccattta aatcgagcag
      781 gagcgtttca acaatatttt cttctctttc ttcaatacgt aatgtctcaa cactgattgg
      841 cctccgatca ggcaaatcac caagcaaacg aaacagttgc tttcttcttt cgactctcat
      901 cccatcaccc ttcctttttc gcttacttca catttacgag acagatctgg gttccccttt
      961 atttttccaa cataaatcac atcatttact aaatatttag taaatataac gaaatattcc
     1021 gaaaacaatt attgtaaccg cttactttta tatgatagta tcaatttatc aaaaacagat
     1081 gagttaatat tttactaaat agatgagagg gatacccatg gttcgtatta aagatatcgc
     1141 cttgaaagct aaagtttcca gcgcaactgt gtccagaatt ttaaatgaag atgagtcgct
     1201 ttctgttgcg ggcgaaacga gacaaagagt catcaacatc gctgaagagc ttggttatca
     1261 aaccgttgcc aaacgccgaa aatcccgcgg gcaaaaacag cgggctcagc cgctgatcgg
     1321 tgtgttgagc tgtctgtccc ctgatcagga aaggcaggac ccttattttt cttccattcg
     1381 gaaagggatt gaaaaggaat gctttgaaca ggaaattttc attacaaatt cgattcatct
     1441 cggctccttt caggaacata tctttcggga attggatggt gtcattgtca tcggccgtgt
     1501 tcatgatgaa gcggttaagc atatcagcgg gaggctggag catgccgtat ttatcaatca
     1561 ttcaccagat ccgcaagcat acgattcgat tggaatcgat tttgaatcgg cttcacgcca
     1621 ggcgattgat caccttctcg acttaggcta caaacggtta ggctacattg gcggacaaga
     1681 aaaagagcat acgctgaagg acggccaaag cattcgcaga acgattgaag ataaacgcct
     1741 gaccgctttt ttggagtcag ccgcccccca gcctgagcat gtgctgatcg gagaatacag
     1801 catgcgtgag ggctatcgcc tgatgaagaa agcaatcgat caaggccatc tgccggaagc
     1861 attctttatt gccagcgatt ctatggcgat cggcgcatta aaagcgcttc aggaagccgg
     1921 actgcaagtg ccgcgggata ccgcaatcgt cagctttaac ggcattgagg aagctgaatt
     1981 tgccagcacg cctttaacga cggtgaaggt atacacagag gaaatgggcc ggacaggcgt
     2041 aaaactgctg cttgaccgtc tcaatggccg aacgcttcct caccatgtca ccctgcctac
     2101 aacattaatc gtaagacaaa gctgcggatg tacagcaaag gaggtgacat aagcaaagat
     2161 tcatcacgat gataaggagg aaaagatgaa acacactttt gttttatttc tctctcttat
     2221 tctgcttgtt ctgcccgggt gttcagcaga gaaaagctca gcagataccg caaaaaagac
     2281 gttaaccatt tattctacaa tgtcaaccga cagtgaaaga gatacgttca gaaaactagc
     2341 ggcagcattc gaaaaggaac acagtgacat tcatgtcagc cttcatttcc caggcaatga
     2401 ctatgaaaat atgatgcgtg tcagaatggc ggccaatgat ttgccagatc tattcgatac
     2461 acatggctgg gggaaaatca ggtacggtga atatacagcg gatctccggg atatggaatg
     2521 gactcaagat cttgatccca atttaaacag catcctcaaa aataaaagcg gaaaggtgta
     2581 tgcctatccg atcaatcagg caaaagacgg tctggcgtat aaccgcaata ttttagaccg
     2641 ttacggcatt gctccgccgg aaaccatgga tgactttatc aaagcactga gaacgattaa
     2701 agaaaagagc aagggaagca ttgttccctt ctggtttgcc ggctatgaca aaagctcatt
     2761 cgctcaatat tacgaccaat tcgctacacc tcttctcatc acagaccttg cccataatga
     2821 aaaaaaacag ctcatcaatg gcacttttca atggagtaaa ttcacttatt tatcagaaat
     2881 cctcaaacaa atgcagaaag aaaaattgat taacatcgac gccgtcacgg caaaaaaatc
     2941 acagctcatt gaattaatgg cccaaaacaa aatcgccttt acaatgcaag gcggcaccct
     3001 cggccaagac gttgcccaga tcaatccgaa cgtcaaggtc ggcattatcc cgacacccgc
     3061 catacatccc ggtgataacc cgatatggat cggcggtgaa cgctacaccc ttgcagcatg
     3121 gaaagattcg cctcaattaa aagaggcaaa agaatttatc gcatttatgg cccgcccggc
     3181 caatgccaaa caaatggctg aagccacatc gcttccttca gggctgacca atgtgaaagc
     3241 tgatattttc tacgcaaacg attatgagca ttatcaagat gtcaaagtcg agccttactt
     3301 cgaccgttta tacttgccaa acggaatgtg ggatgtcttg ggcacggttg ggcaggagct
     3361 ggcggctgac attatggcgc ctcaagaaat ttcgcagaaa ctgggaagag aatataaacg
     3421 actcagggag caatcggaaa cacagggagc tgaaaacaat gagtgagatc gcaagggacg
     3481 ttcatgtgaa acaggttaag ccaaaaaagc agtcctcact ttggtggatg tacattcctg
     3541 ctttgctctc cgttctggtc tttatgattt atccgtttgt gaaaggcaca ttgattacct
     3601 ttacaaactg gaacggcttt tcacaagttt accaatgggt tggattcgcg caatatgaaa
     3661 ggctgttttc tgatcctgac acttggcata tcttaaaaaa cactctgctt tacgggcttg
     3721 ggagcacctt ttttcaaaat gtggtcgggc ttctgtacgc tctgctctta aatcaaagca
     3781 ttaaaacgaa agcggtaaca agaacaattg tgtatttgcc ggtgatgatc agcccgctca
     3841 ttatgggcta catttggtac tttttcttct cctatgacgg aggcgcgctc aacgatttgc
     3901 tcggggtctt cggcatcagc cctattaatg cgctcgccag tccctcgctc aatccatgga
     3961 tcattgtcat gattaacacc tatcaatatg tcgggatcgc tatggttgtc tacttagccg
     4021 gtctgcaaag cattccgaaa gactactatg aagcggcgca aatggatggc gcgaaacaag
     4081 gccagcagtt ttttaccatc acgctgccgc tgttaatgcc ctccattacc attaatatgg
     4141 tcatcaacat tatcggaggc ttaaagctgt ttgacgtcat cattgcactc accgctggcg
     4201 gccccggaaa tgcgtcacaa tccatgtcca catttatgta tgatttgtat ttcaaacggc
     4261 aggatgccgg ctatgccgca acgcaaggca tatttatggc atttgtcatt ttgatcatca
     4321 gcttttgcgc gcttgcgtac tttaaaagaa aggagacgga aatgtcatga gagccgcccg
     4381 tacaaaaagc atgcggatca ttacgcttct tgcagccatt gtggcctgtg cgcattttat
     4441 tcctttttat atcctgctga ccacttcatt gaaagcaaaa ggagactaca gttcgaaatg
     4501 gatatttcca gccgacatct cctttcataa tttttcagag gcgtgggagc gcgcttcgct
     4561 aggaaactct tttataaaca ccatgatcat cacaggtttt tctgccttgt tattaattat
     4621 attcggctca cttgccgcct acccgcttgc ccggcgggaa acgaaactga ataaagccgt
     4681 ttttgccttg ctgatttcca ttatgatcat ccctccgtta acgtccatgg ttcctttgta
     4741 ccgaatggtc gtggacgccg gaatggtcaa tacacacgca atcgccattt tcatcaatac
     4801 agcggcttac atgcctttaa ccgtattctt atattcaggc tttatccgat cgaccattcc
     4861 aaaagagctt gaagaagccg caagaattga cggcgcaggc atgctgaaaa tcttttttac
     4921 gattgtgttt cctctgctga agccgatcac tgcgaccatc tgtattattt cttgtgtctt
     4981 tatttggaac gactatcaat ttgccatttt ctttttacaa gatcaaaagg ttcagacatt
     5041 aacagtagcc atggcaggtt ttttcggaga aaacgcaaac aaccttcatt tagttgccgc
     5101 agcggcactt atggcaatgc tgccgatggt tgttctgttt ttggcgctgc aaaaatactt
     5161 tattgccggc ctgtcttccg gagcggtaaa gggttaacat taaaggagga agcaatatga
     5221 aaaagattac atttatcggt gcagggagca cgattttcgc caaaaatgtt ttgggagact
     5281 gcctgttaac agaggcactg aatggatttg agttcgctct ttacgacatt gatcccaaac
     5341 gcctgcagga atcccagctc atgctcgaaa atctaagaga ccgttataac ccaagtgtgg
     5401 ccatcaacag ctatgatgac agaaaactcg ccttgcaaaa cgcaggctat gtcatcaatg
     5461 cgattcaggt tggaggatac aaaccgagca cagtcatcga tttcgagatt cctaaacggt
     5521 atgggctgcg ccagacgatc gccgacacag tcggcatcgg cgggatattc agatctctca
     5581 gaacaatccc ggtcttattt gatatcgcaa aagatatgga ggaaatgtgc cctgacgcat
     5641 ggttcttaaa ctatacaaat ccaatggcca ctcttacagg cgccatgctc cgctatacca
     5701 atatcaaaac aatcgggctc tgccacagtg ttcaagtgtg cacgaaggat ttatgtaaag
     5761 ctctcggaat ggagcatgac ggaattgagg aacgcatcgc aggaatcaat catatggctt
     5821 ggcttttgga agtcaaaaaa gacggcacag atttatatcc agaaatcaaa aggagagcga
     5881 aagaaaaaca aaaaacaaag catcatgata tggtgcggtt tgaattaatg gataagttcg
     5941 gctattatgt cacggaatcg tccgaacata acgcagagta tcacccatac tttattaaac
     6001 ggaattaccc tgaattgatc agcgagctgc aaatcccgct cgacgaatat ccgagaaggt
     6061 gcgtcaagca gattgaaaat tgggagaaaa tgcgggatga tatcgtcaat aataaagacc
     6121 ttacgcacga acgctcaaag gaatatggtt caagaattat agaagcgatg gaaacaaacg
     6181 aaccattcac cttcggagga aacgtcttga atacagggct gatcaccaac cttccttcaa
     6241 aagcggttgt ggaagtgaca tgcgtcgccg acagaaaaaa aattacaccg tgtttcgccg
     6301 gagaactgcc tgagcagctt gcagccttaa accggacgaa tattaataca cagctgatga
     6361 caatcgaagc ggctgtcaca caaaaaaaag aagcggttta ccaagctgca atgcttgatc
     6421 cgcatacaag cgcggagctt tccatgaagg acatcatttc catgtgtgac gatttatttg
     6481 cagcacacgg cgattggctt ccggaataca aataa
//
