LOCUS       YYYXYLRN                6760 bp    DNA     linear   BCT 05-FEB-1997
DEFINITION  Thermophillic bacterial sp. ORF1, 3' end; complete ORF2; xylose
            repressor protein (xylR) gene, complete cds; xynA gene, complete
            cds; complete ORF5; ORF6, 5' end.
ACCESSION   L18965
VERSION     L18965.1
KEYWORDS    repressor protein; transport protein; xylR gene; xynA gene.
SOURCE      Caldicellulosiruptor sp. Rt8B.4
  ORGANISM  Caldicellulosiruptor sp. Rt8B.4
            Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
            Thermoanaerobacterales Family III. Incertae Sedis;
            Caldicellulosiruptor; unclassified Caldicellulosiruptor.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Dwivedi,P.P., Gibbs,M.D., Saul,D.J. and Bergquist,P.L.
  TITLE     Cloning, sequencing and overexpression in Escherichia coli of a
            xylanase gene, xynA from the thermophilic bacterium Rt8B.4 genus
            Caldicellulosiruptor
  JOURNAL   Appl. Microbiol. Biotechnol. 45 (1-2), 86-93 (1996)
   PUBMED   8920183
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Caldicellulosiruptor sp. Rt8B.4"
                     /mol_type="genomic DNA"
                     /strain="RT8.B4"
                     /db_xref="taxon:28238"
     CDS             <1..423
                     /note="putative membrane bound sugar transport protein;
                     ORF1; putative"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42041.1"
                     /translation="DPNVAFYSVVAVICWQYIPFYMIFFIAALSNIPQELYEAAKIDGA
                     TQGQYFWRIELPLLTPSIKTACILSLIGSLKYFDLIYVMTEGGPSNATELMATYMYKNA
                     FASFKMGYGSTIASAMFLIITTAGIFAYFVTRRKEE"
                     /product="gnl|TC-DB|Q72H67|3.A.1.1.25"
     CDS             456..1283
                     /note="putative membrane bound sugar transport protein;
                     ORF2"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42042.1"
                     /translation="MSFIKETKKWYFIFLAVWTLIADVPFLFMLFTSFKTQSELLSGNT
                     WQIPRQPTIGNFSTVLEGNFFTYLKNSVIAVSISVVLILIISSMAAFAFSRFKFALNNL
                     LYSLIIAGMAIPIHVTLIPIYVLTNKIKLYDTVFALIGPYVALSLPMSIFILTEFMREI
                     PLELEEAAKIDGCSMFRLYSDILLPLSAPALITVGIYNGTYLWNEFVFALVLTSSPTRR
                     TLPLGIWDFQARYGSDIPAIMAFLTLSLLPMLIAYIFGQDKIIKGMMAGAVKG"
                     /product="gnl|TC-DB|G4FGN6|3.A.1.1.41"
     gene            1371..2570
                     /gene="xylR"
     CDS             1371..2570
                     /gene="xylR"
                     /note="putative xylose repressor gene; putative"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42043.1"
                     /translation="MGNHALIKQINKLLILKTILDHRTISRAKISKLVDLNKATVSNLT
                     DELIKEGFVIEKGYGHSKGGRRPVLLEVNKNVGLIIGIDLGVNYIHLVLTNFIGEIIWE
                     RNMAIKLGEKQEKILESLFIILEEAIKAAPPTQKGILGIGIGVPGIVEKNSGTVLLAPN
                     LKWQDVPLKRMVESRFGLPVYIDNEANAGALGEKWFGGWGDVSHLLYVSVGIGIGAGIV
                     IGEEVYRGAKGFAGEVGHMTIDFNDDVCSCGNVGCLENFASERALLSLIKTLVESGVED
                     EYINRDTVEEMDAGYIIQSALQRSRVAMNAITDIANKLGIGIANLVNIFNPDIVVIGNK
                     ASFMGDLFLEELRRIVYKRAFITQYHHVRVEVSKLKDRACVLGCVAMVISDMLAFPDYV
                     "
                     /product="DBD-Pfam|MarR,DBD-SUPERFAMILY|0040266"
     gene            2599..4653
                     /gene="xynA"
     CDS             2599..4653
                     /gene="xynA"
                     /EC_number="3.2.1.8"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42044.1"
                     /translation="MMRSLKSRKLVFILAMLFLINAIVSLKFITYSSANQLASKSKYIE
                     YNFENKSVSPLAKYPSNVVLKVTNSTCAEGTFSVLVAGRKNANDGVIVDITKFLDFSRE
                     YEVSFYVLQTTKKLQRISVTLEILDSNDKNQVIAAEKVLLPNIWTKVSAKVQASNYKKA
                     KRINLIVNMPTSKSDSFYIDLFTIKDLENAYVLKQENFENKNTGGFLPEDKNCKITLAK
                     DRAYSSAYSLKVQPSQKTKNGKILFPIKGLLQKGGTYDFSLLVYQDSSKPVNFSAGIKL
                     NDGKSTKEIVLAKQNVAPKKWTQLFATLDLDTRFSAKDVSFFVKPAAAISYYLDLYSIS
                     DENWGQPVPDYNLPSLCEKYKNYFKIGVAVPYRALTNPVDVEVIKRHFNSITPENEMKP
                     ESLQPYEGGFSFSIADEYVDFCKKDNISLRGHTLVWHQQTPSWFFTNPETGEKLTNSEK
                     DKEILLDRLKKHIQTVVGRYKGKVYAWDVVNEAIDENQPDGYRRSDWYNILGPEYIEKA
                     FIWAHEADPKAKLFYNDYSTEDPYKREFIYKLIKNLKAKGVPVHGVGLQCHISLDWPDV
                     SEIEETVKLFSRIPGLEIHFTEIDISIAKNMTDDDAYNRYLLVQQAQKLKAIFDVLKKY
                     RNVVTSVTFWGLKDDYSWLRGDMPLLSDKDYQPKFAFWSLIDPSVVPKE"
                     /product="CBM22|GH10"
     gene            4887..5498
                     /gene="ORF5"
     CDS             4887..5498
                     /note="ORF5"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42045.1"
                     /translation="MIENFKEDRCIVCHLKNRAMDKFFDDFLYESVNDYSLRDRIRKGG
                     ICPGHARKLESFGDVLAHAIIYSDLLRSFKHNHHIEILPRKKKTQGQNICVFCEKEQGF
                     EDIYTRAFSYFFFTSPQFKSAFAERGFICQRHLNQVLEKTASVSAKRELLSVLESKIDK
                     ILNHLERIKEKNDYRNIHINYTADEIRAWHMAVEFVAGTQ"
     gene            5554..>6760
                     /gene="ORF6"
     CDS             5554..>6760
                     /note="ORF6"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB42046.1"
                     /translation="MRTIEYDVVQDMIRNFTEQLQEISNEINSESIKKLAGSIKEKIEK
                     NAFYLVVLGQFKRGKSTLINYMLGANLLPTGVLPLTSVITKIYYSPEVKVDVIFESGVK
                     KEIPVDELDLYCTERGNPKNQKCVDTIEIGYPFDFLNKDVVIVDTPGIGSVYQHNTDVT
                     YEFIDKSDAVVFVLSVDPPITEVEKQFLLKIAENVDKIFFVINKSDLTSKNEIEEIVSF
                     TTNVIKDITKKGNINIFPLSAKMALEGKISKNEEMIEKSCVEIFEKELKQFLKEEKGKI
                     QILSNLKSLDGFLGVCEAFLENDMKLKIMPVKQLEENIEKFNEFLERVNQNKIEIYKLF
                     KIEMNDILQSFDDEMEKIKKELVVKITKKINDYYPSVARLKRIEQKEHLNKYLEKAIVE
                     EFD"
ORIGIN
        1 gatcccaatg tagcttttta ttcagtggtt gctgttatat gctggcaata tatacctttt
       61 tatatgatat ttttcatagc agctctgtca aatatacctc aggaactcta tgaggctgca
      121 aagatagatg gtgcaacaca ggggcagtat ttctggagaa tagaacttcc tcttttaaca
      181 ccttccataa aaacggcctg cattttatct ctaattggtt cattaaaata ttttgattta
      241 atttacgtta tgacagaagg tggtccttca aatgcaacag aattaatggc tacatatatg
      301 tacaaaaatg ctttcgcgtc attcaagatg ggatatggaa gcactatagc atctgctatg
      361 tttcttataa tcacaactgc cggcatattt gcttattttg taacaagacg caaggaagaa
      421 tagcttttac attctgtaag ggggttagat attcaatgag tttcataaag gagactaaaa
      481 aatggtattt tatatttctg gcagtatgga cattaattgc agatgttcca tttttgttta
      541 tgctgtttac ttcatttaaa acacagtcag aacttttatc tggcaacaca tggcaaatac
      601 caagacaacc aactataggg aatttttcaa ccgtgttgga gggtaatttt tttacttatt
      661 taaaaaacag tgtaatagcg gtctctatat cggttgttct gattttaata atatcttcaa
      721 tggctgcttt tgctttttcc aggtttaagt ttgctcttaa caatctactg tattcgctta
      781 taattgcagg catggcaata cctattcatg taactttaat accaatttat gtgttaacaa
      841 acaagattaa gctttacgat acggtatttg ctttgattgg tccatatgtt gcactcagtt
      901 taccaatgtc gatttttata ttaaccgagt ttatgcgtga aatacctctt gaactcgaag
      961 aagctgctaa aatagacggt tgttcaatgt tcaggttata ttctgatatt ttattaccct
     1021 tatcagcacc agctttgata actgttggta tttacaatgg aacatatctc tggaatgaat
     1081 ttgtgtttgc acttgttttg acaagttcac cgacaagaag aactttaccg cttggcattt
     1141 gggactttca ggcaagatac ggctctgaca ttcctgccat aatggcattt ttgacattat
     1201 cacttttgcc aatgctaatt gcatacatct ttggtcagga caagattata aagggtatga
     1261 tggcaggagc tgttaagggt taaaccagtg gtttgagggt atttcattgt ttgaggattt
     1321 tacattataa tatttttcaa accacaatta gaataaagga taaagggaag atgggaaatc
     1381 acgcattaat taagcaaatc aacaagctgc tgattttaaa aacgatattg gatcatagaa
     1441 caatttcgag agctaaaatc tcgaaacttg tagatttaaa taaagcaact gtatcaaatc
     1501 ttactgatga gctcataaaa gagggatttg tgatagaaaa aggatacggg cattcaaaag
     1561 gtggaagaag gccggtctta cttgaagtta acaaaaatgt gggactcatc attgggatag
     1621 accttggtgt taactatatt catttggttt tgacaaattt tattggtgag attatctggg
     1681 aaagaaatat ggctatcaaa cttggtgaaa aacaggaaaa gattctggaa agccttttta
     1741 taatacttga agaagcaata aaagctgctc caccaacaca aaagggaata cttggaatag
     1801 ggataggagt tccaggtatt gttgaaaaaa actctggaac tgtgctttta gcaccaaatt
     1861 tgaaatggca ggatgtacct ttgaaaagga tggttgaaag caggtttggg cttccagtat
     1921 atatagacaa tgaagcaaac gcaggtgctt tgggcgaaaa atggtttggt ggatggggag
     1981 atgtttccca ccttttatat gtaagtgttg gcataggtat tggtgccggg attgtaattg
     2041 gtgaagaggt ttacagaggt gcaaaggggt ttgctggaga agttggacat atgacaattg
     2101 attttaacga tgatgtatgc agctgtggta atgtcgggtg cttagagaac tttgcatcag
     2161 aaagggctct tttgagtctt ataaagacac ttgtggaaag tggagtagaa gatgaatata
     2221 taaacaggga tacggttgaa gagatggatg caggttatat aatccagtct gccttgcaaa
     2281 gaagcagagt agctatgaat gctatcacag atattgctaa taagcttggt ataggtattg
     2341 ccaatttggt caatatattt aatcctgata tagttgtaat aggcaataaa gcatcattta
     2401 tgggtgattt gtttttagaa gagctgagaa gaattgtata taaaagagca tttataaccc
     2461 agtaccatca tgtaagagtt gaagtttcaa agctaaaaga cagagcctgt gtactgggat
     2521 gtgttgcaat ggtgatttct gatatgcttg cattccctga ttatgtataa gaaaagtttt
     2581 aaaaggagtg tttatttgat gatgagaagc ctgaaaagtc ggaagctggt ttttattctt
     2641 gccatgctgt ttttaataaa cgccatagta tcattgaaat ttataactta ttcatctgca
     2701 aaccaattgg cttccaaaag taaatacatt gaatacaatt ttgaaaacaa aagtgtctct
     2761 ccacttgcaa aatatccatc caatgttgta ttaaaggtta caaattctac ttgtgcagag
     2821 gggacgtttt cggttctggt agcaggaaga aaaaatgcaa atgacggagt tatagttgat
     2881 attacaaagt ttttagactt ctcaagagag tatgaagttt ctttttatgt tttacaaacc
     2941 acaaaaaaac ttcaaagaat ttctgtaacc ttagaaattt tagattcaaa tgataaaaat
     3001 caggttattg ctgctgaaaa ggtgcttttg ccaaacatat ggacaaaggt gagtgcaaaa
     3061 gttcaggctt caaattataa aaaggcaaag aggatcaatt tgattgttaa catgccaaca
     3121 agcaaaagcg actcatttta cattgacctg tttacaataa aggacttgga aaatgcttat
     3181 gttctcaaac aggagaattt cgaaaacaaa aatactggcg gttttttgcc tgaagataaa
     3241 aactgcaaaa taacacttgc aaaggataga gcttacagca gcgcttacag tctcaaagtt
     3301 cagccatctc aaaagaccaa gaatgggaag attctctttc caataaaggg cctgctgcaa
     3361 aagggaggga catatgattt ttcactgctt gtttaccagg acagttcaaa gcctgttaat
     3421 ttttcagcag gcataaagct aaacgatgga aaatcgacaa aagagattgt tttggcaaaa
     3481 cagaatgttg cacccaaaaa gtggacgcag ctttttgcaa ctcttgatct tgacaccagg
     3541 ttttctgcaa aagatgttag cttttttgta aagccagctg ctgcaatctc ctattatttg
     3601 gacctttatt caatctcaga tgaaaattgg gggcagcctg tgcctgatta taatctgcca
     3661 tcactttgtg aaaagtacaa aaactatttc aagattggag ttgctgtgcc ctacagggct
     3721 ctgacaaatc cagttgatgt ggaagtgata aaaaggcatt tcaacagcat aacaccggag
     3781 aacgaaatga aaccagagag ccttcagccc tatgaaggtg gttttagctt tagcattgca
     3841 gatgagtatg tagatttttg caaaaaggac aatatctcgc tgcgagggca cacccttgtt
     3901 tggcatcagc aaaccccgag ctggttcttt acaaatcctg aaacaggcga aaaacttact
     3961 aacagtgaga aggacaaaga aatactattg gataggctaa agaagcatat ccagacagtt
     4021 gttggcaggt ataaggggaa agtatatgca tgggacgttg tgaatgaggc gattgatgag
     4081 aatcagccgg atgggtatag aagaagtgac tggtacaata tcttgggacc ggagtacatt
     4141 gaaaaggcat ttatctgggc gcatgaagca gacccgaaag caaagctttt ctacaatgac
     4201 tacagtacag aagacccata caagagagaa tttatataca aactaattaa aaatttaaaa
     4261 gcaaaaggtg tgccagttca tggtgttggg cttcagtgcc atatttcact tgactggccg
     4321 gatgtgagtg aaattgagga gactgtcaaa ttatttagca ggattccagg acttgaaata
     4381 cacttcacag aaattgatat aagtattgct aaaaacatga ccgatgatga tgcatacaat
     4441 cgctatcttt tggttcagca ggcacaaaaa ttaaaagcaa tttttgacgt tttgaaaaag
     4501 tacagaaatg tagttacaag tgttacattc tggggactga aggatgatta ctcatggcta
     4561 cggggagata tgccactttt gtccgacaaa gactaccagc caaagtttgc gttctggagc
     4621 ttgattgacc catcagttgt cccaaaagag taataaaact aattattgac taaaatatca
     4681 aaacttcata taataaaatt gaaaagaaaa agtgaatata gaggcgatgg agttcgccta
     4741 aaaatgccac ggcaatagaa aatggcttgt ggctgataac tcctgtccgt atttaaaaag
     4801 atgcgggcag gagttttttg cttttaataa gctcttattt ttgagtactc tgggggggat
     4861 tgaccttgga catagtgtat tttgagatga tagaaaactt caaagaagac aggtgcattg
     4921 tatgccatct taaaaacagg gcaatggata agttttttga tgattttctg tatgaatcag
     4981 taaacgacta cagcttacgt gacagaataa gaaagggtgg aatttgtcca gggcatgcaa
     5041 gaaagttgga gtcgtttgga gatgtgcttg cccatgccat tatatattcg gatttgctga
     5101 gaagtttcaa acacaaccac catattgaga tattgcctcg caaaaagaaa actcaaggac
     5161 aaaatatttg cgtcttttgt gaaaaagaac aaggctttga agacatctac acaagagcat
     5221 tttcctactt ttttttcact tcacctcaat ttaaatctgc atttgctgaa agaggtttta
     5281 tatgtcagag gcacttaaat caagttctgg aaaagacagc ttctgtatct gctaaaagag
     5341 agcttttgtc agttttagaa tccaagatag acaaaatctt gaatcatctt gagagaataa
     5401 aagaaaaaaa tgattatcga aatattcaca taaactatac agctgatgaa atcagggcat
     5461 ggcacatggc tgttgagttt gtggcaggta cacagtagtt tattatcaga cacgcaaata
     5521 ttttacaata atgataaaat gaggtgattt aggatgagga ctatagaata tgatgttgtg
     5581 caagatatga ttagaaactt tactgagcaa ctccaggaga tttcaaatga aatcaattca
     5641 gaatctatta aaaagttagc agggagtatt aaggaaaaaa ttgaaaagaa tgcattttat
     5701 ctggttgtat tgggtcagtt taagagggga aaatcgacgc ttataaatta catgcttggt
     5761 gcaaatttac taccaaccgg agttcttcct ttaacctcag taataacaaa gatttattac
     5821 agtccggagg ttaaagtgga tgtaattttt gaaagtggtg taaaaaaaga aattccagtt
     5881 gatgaacttg atctatattg tacagaaaga gggaatccta aaaatcaaaa atgtgttgat
     5941 acaattgaga taggttatcc ctttgatttt ttgaataaag atgttgtaat tgttgacaca
     6001 cctggaattg gttctgtata tcagcacaac acagatgtga catatgaatt tattgacaag
     6061 tcagatgcgg ttgtgtttgt actgtctgtt gacccaccaa ttactgaagt tgaaaaacag
     6121 tttctactca agattgctga aaatgtagat aaaatattct ttgtaattaa caaatctgac
     6181 ttgacaagca aaaatgaaat agaagagata gtgagtttta caactaatgt aatcaaagat
     6241 attacaaaaa aaggaaatat aaatatcttt cctctttcag caaagatggc acttgaaggg
     6301 aagatatcta aaaatgaaga aatgattgaa aagagctgcg tagaaatttt tgagaaagaa
     6361 ttaaaacaat ttttaaagga agagaaggga aaaatacaaa tcttgagcaa tctaaaaagc
     6421 ttagatggtt ttttgggagt ttgcgaagcc tttttagaaa atgatatgaa actcaagatt
     6481 atgcctgtaa agcaacttga agaaaatatt gaaaaattta atgagttttt ggagagagta
     6541 aatcaaaaca aaattgaaat ttataagcta tttaaaattg agatgaatga tattttacaa
     6601 agttttgatg acgagatgga gaaaataaag aaggaacttg tagttaagat aactaaaaag
     6661 ataaatgatt attatccatc agttgcaaga cttaaaagaa ttgaacaaaa agagcatctt
     6721 aataaatatc ttgaaaaggc tattgtggaa gagtttgatc
//
