LOCUS       AM886293                6013 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Escherichia coli 1540 plasmid pIP1206 complete genome.
ACCESSION   AM886293
VERSION     AM886293.1
KEYWORDS    complete genome.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Perichon,B., Bogaerts,P., Lambert,T., Frangeul,L., Courvalin,P. and
            Galimand,M.
  TITLE     Sequence of conjugative plasmid pIP1206 mediating resistance to
            aminoglycosides by 16S rRNA methylation and to hydrophilic
            fluoroquinolones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Perichon,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2007) Perichon B., Unite des Agents
            Antibacteriens, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris,
            FRANCE
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pIP1206"
     gene            1..1021
                     /gene="RafR"
                     /locus_tag="IPF_354"
     regulatory      1..6
                     /regulatory_class="ribosome_binding_site"
                     /gene="RafR"
                     /locus_tag="IPF_354"
     CDS             11..1021
                     /gene="RafR"
                     /locus_tag="IPF_354"
                     /note="Highly similar to helix_turn _helix lactose operon
                     repressor; transcriptional regulator PurR [Escherichia
                     coli]"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAP07693.1"
                     /db_xref="GOA:B1VC71"
                     /db_xref="InterPro:IPR000843"
                     /db_xref="InterPro:IPR001761"
                     /db_xref="InterPro:IPR010982"
                     /db_xref="UniProtKB/TrEMBL:B1VC71"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGYR
                     PNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDEHA
                     DCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDFDNH
                     AGTGQATQKLIALGHKRIALLSEDTSHTYVIARRQGWLDALNEHELEDTWLRLVSPTRR
                     AGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKAAVSLVVYDGLPQDSI
                     IELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHSPCS"
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
     gene            1104..3243
                     /gene="RafA"
                     /locus_tag="IPF_353"
     regulatory      1104..1109
                     /regulatory_class="ribosome_binding_site"
                     /gene="RafA"
                     /locus_tag="IPF_353"
     CDS             1117..3243
                     /gene="RafA"
                     /locus_tag="IPF_353"
                     /note="Highly similar to Alpha-galactosidase (EC 3.2.1.22)
                     (Melibiase)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAP07694.1"
                     /db_xref="GOA:B1VC72"
                     /db_xref="InterPro:IPR000111"
                     /db_xref="InterPro:IPR002252"
                     /db_xref="InterPro:IPR013785"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="UniProtKB/TrEMBL:B1VC72"
                     /translation="MVSKYCRLSSPRSDLIIKTHPHAEIIWWGSALKHFSPDDCASLER
                     PVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDSSPVFYTVDVEHTENTLRLTSE
                     DSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFHGRW
                     TREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWSGNHR
                     MRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYHRFLRD
                     EIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFKGRNDDR
                     AALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPDWVLSMPG
                     YSQPTGRYQYVLNLNIPEAFAYIYERFSWLLGEHPVDYVKWDMNRELVQAGHEGRAAAD
                     AQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNNDALERCTIQR
                     GMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVAADAKESDGYR
                     RYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQLAMPDYTLPGIL
                     RFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGEWLAQGGIQLPVL
                     DPESAILIALERAV"
                     /product="GH36"
     gene            order(3291..3296,3306..4583)
                     /gene="RafB"
                     /locus_tag="IPF_349"
     regulatory      3291..3296
                     /regulatory_class="ribosome_binding_site"
                     /gene="RafB"
                     /locus_tag="IPF_349"
     CDS             3306..4583
                     /gene="RafB"
                     /locus_tag="IPF_349"
                     /note="Highly similar to Raffinose permease [Escherichia
                     coli]"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAP07695.1"
                     /db_xref="GOA:B1VC73"
                     /db_xref="InterPro:IPR000576"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="InterPro:IPR018457"
                     /db_xref="InterPro:IPR020846"
                     /db_xref="InterPro:IPR022814"
                     /db_xref="UniProtKB/TrEMBL:B1VC73"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLSK
                     TDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLLRF
                     NIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCATMAGM
                     LFNVDPSLVFWMGSGGALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFSLFRM
                     RQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNAIIMFC
                     TPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLVGAFKYI
                     TAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIALTVTVIS
                     AFTLSSSRGSVHPSVEKAPAAHSEIN"
                     /product="gnl|TC-DB|P16552|2.A.1.5.2"
     gene            4570..4575
                     /gene="RafD"
                     /locus_tag="IPF_347"
     regulatory      4570..4575
                     /regulatory_class="ribosome_binding_site"
                     /gene="RafD"
                     /locus_tag="IPF_347"
     gene            4583..6013
                     /gene="RafD"
                     /locus_tag="IPF_347"
     CDS             4583..6013
                     /gene="RafD"
                     /locus_tag="IPF_347"
                     /inference="similar to sequence (same
                     species):UniProtKB:P16553"
                     /note="Very similar to sp|P16553|RAFD_ECOLI Raffinose
                     invertase (EC 3.2.1.26) (Invertase)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAP07696.1"
                     /db_xref="GOA:B1VC74"
                     /db_xref="InterPro:IPR001362"
                     /db_xref="InterPro:IPR006232"
                     /db_xref="InterPro:IPR008985"
                     /db_xref="InterPro:IPR013148"
                     /db_xref="InterPro:IPR013189"
                     /db_xref="InterPro:IPR018053"
                     /db_xref="UniProtKB/TrEMBL:B1VC74"
                     /translation="MKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLIYF
                     NGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPVALAPGDKYDRDGCFSGSAVDD
                     DGVLSLIYTGHICLEDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFRDPKV
                     WHEEGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGESYMWECPDFFRCG
                     NFHWLMFSPQGMTPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDFYAPQSM
                     LAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREVESLRRKC
                     QPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDNQAGRLVLW
                     RYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYPQADSRQLSL
                     YAAHGDAILTNGTLWMLT"
                     /product="GH32"
ORIGIN
        1 aggagataat atgtcactta aggcgattgc cactgcactc ggagtttctg tcaccactgt
       61 cagtcgggct cttggaggct atgcagatgt gtcagcttcc acccgtgcac gggtggaagc
      121 tgaagctcgt cgacgcggct accgtcctaa tactcaggca agaagactca aaaccggtaa
      181 aaccgatgct gtcggtctgg tttatcctgg acgtgatgtg ccatttaaca gtggtgtttt
      241 tatggatatg gtcagttgca tcagccggga acttgctcac catgatattg acttactact
      301 gatagctgat gatgagcatg ccgattgcca cagctatatg cggcttgttg aaagtcgcag
      361 ggttgatgct ctcatcattg cacacactct ggatgacgat ccccgtatca cacaccttca
      421 taaagcaggt attccatttc tggctcttgg tcgggtacca tctggtctgc cctgtgcgtg
      481 gtttgacttt gataatcatg ccgggactgg acaggcaacc cagaaactga ttgctttggg
      541 acataaacgt attgcattgt tgagtgaaga cacttcacat acctatgtta ttgcaagacg
      601 tcagggatgg cttgatgcac tgaatgagca tgaactggaa gatacatggc tgcggctggt
      661 ttctcccaca cggcgagcag gatatcaggc cgtgatggag ttaatgtcac taccgatacc
      721 accaacagct attattactg acaatgatct gagtggagat ggtgctgcca tggcgctgca
      781 gttgagcggt cgtctttcag gaaaagctgc ggtatctctg gttgtatatg atggtttgcc
      841 acaggacagt attattgagc tggatgtagc tgcagttatt cagtcaacac gaagcctcgt
      901 tggtcgccag atttccgaga tgatatatca gataattact gattcatcat caaaaccact
      961 acaggttgtc tggaacccgg tattttatcc gggaaagacg attcattctc cttgctcctg
     1021 atttttttat ccagctcaca tatccgaaac gttttggttg atacccgaaa cgtttcggat
     1081 caacagtaag gcatccctga gaaaggagaa tgtcttatgg tttcaaagta ctgcagactg
     1141 agcagtcctc gctctgattt aattattaaa acccatccac atgcagaaat tatctggtgg
     1201 ggctctgcac tgaaacattt ctcaccggat gactgtgcca gcctggaaag accggttgcg
     1261 aatggtcgtc tggatattga tacgccactg acactgatgg ctgaaaatgc cctgggactt
     1321 tttagctctc cgggactgga aggacacagg aatgggctgg attcatctcc tgttttttat
     1381 acagttgacg tggaacatac cgaaaacact ctgagactta ccagtgaaga ttcggtagcc
     1441 ggcctgcgtc tggtcagcga gctggtgatg acgccatcag gaattctgaa agttcgtcat
     1501 gcactgacca acctcagaga gggggactgg cagataaatc gtttcgccat cactttacct
     1561 ttagcggagc gtgcggaaga agtcatggct tttcacggac gctggactcg tgaatttcag
     1621 ccgcacaggg tacgtctcac tcatgatgct tttgttctgg aaaatcgcag agggcggaca
     1681 tctcatgagc attttccggc gctgattgtc ggcacgccag gcttctcgga acaacaggga
     1741 gaggtatggg ctgtgcatct ggggtggagt ggaaatcacc gtatgagatg tgaggcaaaa
     1801 actgacggca ggcgttacgt tcaggctgag gctctgtgga tgccgggtga gaaggctctc
     1861 aggaagaatg aaaccctgta caccccgtgg ctatatgcct gccactctgc ggatggcctg
     1921 aatggaatga gtcagcaata ccatcgtttt ttgcgtgatg aaattatccg tttccctgag
     1981 caaaaacccc gccccgtaca tctcaatacc tgggaaggta tttatttcaa tcacaatcct
     2041 gattacatca tgcagatggc tgagcgtgca gcagcactgg gcgttgaacg tttcattatt
     2101 gatgatggct ggtttaaagg acgtaacgat gaccgcgcgg ctctgggcga ctggtatacc
     2161 gatgaacaga aatacccgaa cgggctgatg ccggttatta aacatgtgaa atctctcggt
     2221 atggaatttg gcatatgggt tgagccggaa atgattaacc cggattctga cctgtttcgt
     2281 cttcatcctg actgggtatt gtcaatgcct ggatattccc agccaaccgg aagatatcag
     2341 tatgttctta acctgaatat tcctgaggcc tttgcttaca tttatgaacg tttctcatgg
     2401 ttactgggag aacatccggt tgattatgtg aaatgggaca tgaatcgtga gcttgtacag
     2461 gcagggcatg aaggccgtgc ggcagcagat gcacagaccc gtcagttcta tcgattgctt
     2521 gatctcctcc gtgaacgttt tccacatgtt gagtttgagt cctgtgcttc cggtgggggg
     2581 cgtattgact tcgaagtcct gaaacgcaca caccggttct gggcatctga caataatgat
     2641 gccctggagc gctgcaccat acaacgtggc atgagttact ttttccctcc tgaggtgatg
     2701 ggggcacata ttggccatcg ccgctgccat gcaactttcc gacagcacag catcgctttt
     2761 cgtgggctga cagcattgtt cggccatatg gggctggagc tggatccggt ggccgcagat
     2821 gcgaaggaat ctgacggtta tcgccggtat gccttgctct ataaagaatg gcgacaactg
     2881 attcatacag gtgttctctg gcgtgtggat atgccagatc cttcgataca ggttcaggga
     2941 gtcgtcagcc ctgatcagtc tcaggcactt tttatgatca gccagcttgc aatgccggat
     3001 tacaccttac caggcatact tcgttttccc ggtctggcgg cggaagtgcg ttaccggctt
     3061 cgggttattg atcacccgga cctccaggtg gtcggtgaag gcggtcatac catgcgcaaa
     3121 ttacctgtct ggatgaatca gagccttgag gccagtggtg aatggctggc acagggaggg
     3181 attcagctcc ccgtactgga tccggagagt gcgattttga tagcacttga aagagctgtg
     3241 tgaagagagg tacggagcct cagctccgtc ctgtttctca atattatccc gtgagggtaa
     3301 gaataatgaa ttctgcaagt acgcataaaa atcccgattt ctggattttc ggtctgttct
     3361 tctttctcta ctttttcatc atggcaacct gttttccgtt tttgcctgta tggctgtccg
     3421 atgtggtcgg gctgagtaaa acggatacag gtatagtctt ttcatgtctt tctctgtttg
     3481 ccatcagttt ccagccattg cttggggtca tatcagatcg cctgggactg aaaaaaaacc
     3541 tgatctggag tatcagcctg ttactggtat ttttcgcccc ctttttttta tatgtatttg
     3601 cgcccctgct gcgcttcaat atctgggcag gcgcactgac tggcggtgtt tttattggtt
     3661 ttgtgttttc tgcaggtgcc ggagctattg aagcttatat agagcgggtc agtcgcagtc
     3721 gtggatttga atacggtaaa gcgaggatgt tcgggtgtct gggctgggcg ttatgtgcga
     3781 ctatggctgg aatgcttttt aatgtcgatc cttctctggt tttctggatg gggtcaggag
     3841 gcgcattatt gttgcttctt ctgttgtttc tggcgcgccc cagtaccagc cagacggcaa
     3901 tggttatgaa tacactgggt gccaattctt ccctgatttc gaccagaatg gtcttcagcc
     3961 tgtttcgcat gcgtcagatg tggatgtttg ttctctacac gattggtgtg gcctgtgtct
     4021 atgatgtatt tgatcagcag tttgccacat tttttcgttc attctttgac actcctcagg
     4081 caggaataaa agcattcgga tttgctacca ctgcggggga gatttgtaat gccattatca
     4141 tgttctgtac accatggata attcatcgca ttggtgccaa aaataccctg cttgttgcgg
     4201 ggggaattat gaccatccgc attaccggtt ctgcttttgc caccaccgcg acagaagtgg
     4261 tgattctgaa aatgcttcac gctcttgaag ttccattttt gctggttggg gcgttcaaat
     4321 atattacggc agtgtttgac acccgactgt cagcgaccgt ttatttaata ggttttcagt
     4381 tttccaaaca acttgctgca atacttctct ctacctttgc cggccacctg tatgatcgta
     4441 tgggattcca gaatacgtat tttgtgctcg ggatgattgc tctgactgtt accgtgatat
     4501 cagctttcac gctgagttct tcccgcggga gcgtacaccc ttctgtagaa aaagcccctg
     4561 cagcgcattc ggagattaac tgatgaaaca gcgtcttgct ttggcacagt ccgcccttga
     4621 aaaactttgc gcacgtcgtg gtaatgcctg gtacccgatt tttcatctgg ctccacctgc
     4681 cggctggatg aatgatccaa atggccttat ttacttcaat gggcgttacc atgcgttctt
     4741 ccagcatcat cctgcaagcg catatcaggg gccaatgcac tgggggcatg ccaccagtac
     4801 cgacatgttg cactggcaac acgaacctgt cgcgctggca cccggagata aatatgatcg
     4861 tgatggctgt ttttcaggga gtgccgtgga tgatgatggc gtgctatcac ttatttatac
     4921 tggtcatatt tgtctcgaag atcgtggtaa tgacagcatt atccgtgaag tacagtgtct
     4981 ggctaccagt catgatggta ttcactttga gaagcagggc tgtgtgctga cacctccgga
     5041 aggtatcatg catttccgtg atcccaaagt ctggcacgaa gaaggctcct ggtggatggt
     5101 cattggtgcc cgggacgctt ctgacaatgg gcaagttctg ttgtatcgcg ggacatcttt
     5161 gcgggactgg catctggagc atgttctcgc tcattccgca gccggagaaa gttatatgtg
     5221 ggaatgcccc gatttcttca ggtgtggtaa ttttcactgg ctgatgttct caccacaggg
     5281 gatgacccct tccggttatc ggttccgtaa cctttttcag agcggtgtgt tggcagggag
     5341 ctggaagcct ggttctgtct ttgcgctgaa agggggattt gaagagctgg attatggtca
     5401 tgacttttat gctccacagt ccatgctggc tgaggacggc aggcgtatca ttatggcatg
     5461 gatgaatatg tgggattcac ccgtgcccac ccgcagtgaa gcctgggcag gatgtctgac
     5521 gctgcccaga gaggtttttg agcgcgatgg ccggctgtgc cagcgacctg tgcgtgaagt
     5581 cgaatctctg cgcagaaaat gccagccatt atcccctgta aggttacagg gtttgcaatt
     5641 actgaccgaa aatgtacagg ccgcagaatt attggtgacg tggcatacgg ttgacagtca
     5701 tgcggagcac tatggcgtcc gccttggaga cggtctgcga ctttatgtgg ataatcaggc
     5761 cggacgactg gtactgtggc gctattaccc tgaggaaggg ctggatggtt accgcagtgt
     5821 tgaacttcct gatacagaat atctgactct tcgtattttc ctggatcgtt catctgttga
     5881 agtgtttgtt aacgatggtg aggcaacctt atcaagtcgt atttatccgc aagcggactc
     5941 gagacaatta tcgttatatg ccgctcatgg cgatgcgata ttaactaatg gcactttatg
     6001 gatgctgacc tga
//
