LOCUS       Z25795                  8517 bp    DNA     linear   BCT 14-NOV-2006
DEFINITION  B.subtilis glycogen operon DNA.
ACCESSION   Z25795
VERSION     Z25795.1
KEYWORDS    1,4-alpha-glucan branching enzyme; ADP-glucose pyrophosphorylase;
            glgA gene; glgB gene; glgC gene; glgD gene; glgP gene;
            glucose-1-phosphate adenylyl transferase; glycogen phosphorylase;
            starch synthase; transfer RNA-Asn; transfer RNA-Glu; transfer
            RNA-Ser.
SOURCE      Bacillus subtilis subsp. subtilis str. 168
  ORGANISM  Bacillus subtilis subsp. subtilis str. 168
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Wawrousek,E.F., Narasimhan,N. and Hansen,J.N.
  TITLE     Two large clusters with thirty-seven transfer RNA genes adjacent to
            ribosomal RNA gene sets in Bacillus subtilis. Sequence and
            organization of trrnD and trrnE gene clusters
  JOURNAL   J. Biol. Chem. 259 (6), 3694-3702 (1984)
   PUBMED   6323435
  REMARK    (sites)
REFERENCE   2
  AUTHORS   Hay,R.E., Tatti,K.M., Vold,B.S., Green,C.J. and Moran,C.P. Jr.
  TITLE     Promoter used by sigma-29 RNA polymerase from Bacillus subtilis
  JOURNAL   Gene 48 (2-3), 301-306 (1986)
   PUBMED   3104146
  REMARK    (sites)
REFERENCE   3  (bases 1 to 8517)
  AUTHORS   Kiel,J.A., Boels,J.M., Beldman,G. and Venema,G.
  TITLE     Glycogen in Bacillus subtilis: molecular characterization of an
            operon encoding enzymes involved in glycogen biosynthesis and
            degradation
  JOURNAL   Mol. Microbiol. 11 (1), 203-218 (1994)
   PUBMED   8145641
REFERENCE   4  (bases 1 to 8517)
  AUTHORS   Kiel,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-1993) Kiel J., State University of Groningen,
            Center of Biol. Sciences, Dept. of Genetics, Kerklaan 30, Haren,
            Groningen, The Netherlands, NL-9751 NN
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Bacillus subtilis subsp. subtilis str. 168"
                     /mol_type="genomic DNA"
                     /strain="168"
                     /isolate="0G1"
                     /sub_species="subtilis"
                     /type_material="type strain of Bacillus subtilis"
                     /db_xref="taxon:224308"
                     /clone="pKBU1 and pKBU101"
                     /clone_lib="Lambda EMBL12 genebank"
     tRNA            <1..40
                     /product="tRNA-Asn"
                     /note="part of trnB region; nt 1-418 also sequenced by
                     Green CJ and Vold BS (1983). Sequence analysis of a cluster
                     of twenty-one tRNA genes in Bacillus subtilis. Nucleic
                     Acids Res. 16:5763-5774 (citation 4)."
                     /citation=[3]
     tRNA            44..134
                     /product="tRNA-Ser"
                     /note="codon recognized: AGC; part of trnB region; also
                     listed in (citation 4)"
                     /citation=[3]
                     /anticodon=(pos:78..80,aa:Ser,seq:gct)
     tRNA            160..231
                     /product="tRNA-Glu"
                     /note="codon recognized: GAA; part of trnB region; also
                     listed in citation 4"
                     /citation=[3]
                     /anticodon=(pos:193..195,aa:Glu,seq:ttc)
     stem_loop       235..263
                     /note="label:T1; putatitive terminator downstream trnB;
                     also listed in (citation 4)"
                     /citation=[3]
                     /function="terminator"
     stem_loop       381..402
                     /note="label:T2; putative terminator downstream from trnB;
                     also listed in (citation 4)"
                     /citation=[3]
                     /function="terminator"
     regulatory      417..424
                     /regulatory_class="minus_35_signal"
                     /note="-35 region of bvx promoter recognized by Bacillus
                     subtilis RNA polymerase containing the sporulation sigma
                     factor E"
                     /citation=[3]
     regulatory      438..445
                     /regulatory_class="minus_10_signal"
                     /note="-10 region of bvx promoter recognized by Bacillus
                     subtilis RNA polymerase containing the sigma factor E."
                     /citation=[3]
     regulatory      448..460
                     /regulatory_class="ribosome_binding_site"
                     /citation=[3]
     gene            464..2347
                     /gene="glgB"
     CDS             464..2347
                     /gene="glgB"
                     /EC_number="2.4.1.18"
                     /function="introduces alpha-1,6-linkages in starch and
                     glycogen"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="first gene of glycogen operon"
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|CBM48|GH13_9"
                     /protein_id="CAA81040.1"
                     /db_xref="GOA:P39118"
                     /db_xref="InterPro:IPR004193"
                     /db_xref="InterPro:IPR006047"
                     /db_xref="InterPro:IPR006048"
                     /db_xref="InterPro:IPR006407"
                     /db_xref="InterPro:IPR013780"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR013783"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="UniProtKB/Swiss-Prot:P39118"
                     /translation="MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWA
                     PHASEVRVAGDFNSWSGEEHVMHRVNDNGIWTLFIPGIGEKERYKYEIVTNNGEIRLKA
                     DPYAIYSEVRPNTASLTYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKKHSD
                     GRHYSYKELSQTLIPYIKKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDL
                     MKFVDECHQQNIGVILDWVPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDL
                     GKPEVHSFLISNALYWAEFYHIDGFRVDAVANILYWPNQDERHTNPYAVDFLKKLNQTM
                     REAYPHVMMIAEDSTEWPQVTGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQ
                     LISFSLLYAFSEHFVLPFSHDEVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKK
                     LIFMGSEFAQFDEWKDTEQLDWFLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQS
                     FEWIDVHNDEQSIFSFIRYGQKHGEALVIICNFTPVVYHQYDVGVPFFTQYIEVLNSDS
                     ETYGGSGQINKKPLSAKKGALHHKPCYITMTIPPYGISILRAVKKRGEIKR"
     regulatory      2327..2337
                     /regulatory_class="ribosome_binding_site"
                     /gene="glgB"
                     /citation=[3]
     gene            2344..3486
                     /gene="glgC"
     CDS             2344..3486
                     /gene="glgC"
                     /EC_number="2.7.7.27"
                     /function="activates glucose-1-phosphate using ATP"
                     /note="second gene in glycogen operon"
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glucose pyrophosphorylase"
                     /protein_id="CAA81041.1"
                     /db_xref="GOA:P39122"
                     /db_xref="InterPro:IPR005835"
                     /db_xref="InterPro:IPR005836"
                     /db_xref="InterPro:IPR011004"
                     /db_xref="InterPro:IPR011831"
                     /db_xref="InterPro:IPR023049"
                     /db_xref="UniProtKB/Swiss-Prot:P39122"
                     /translation="MKKQCVAMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSN
                     CSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTAS
                     SIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRF
                     GIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDI
                     IPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLKEDSELKLFERKWKIYSVNPNQ
                     PPQFISSDAQVQDSLVNEGCVVYGNVSHSVLFQGVTVGKHTTVTSSVIMPDVTIGEHVV
                     IENAIVPNGMVLPDGAVIRSEKDIEEVLLVSEEFVEKELI"
     regulatory      3494..3501
                     /regulatory_class="ribosome_binding_site"
                     /citation=[3]
     gene            3510..4541
                     /gene="glgD"
     CDS             3510..4541
                     /gene="glgD"
                     /function="unknown"
                     /note="third gene in glycogen operon"
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="protein with similarity to ADP-glucose
                     pyrophosphorylase"
                     /protein_id="CAA81042.1"
                     /db_xref="GOA:P39124"
                     /db_xref="InterPro:IPR005835"
                     /db_xref="InterPro:IPR011004"
                     /db_xref="InterPro:IPR011832"
                     /db_xref="UniProtKB/Swiss-Prot:P39124"
                     /translation="MFNNQMLGVIDETTYKHSLQDLTAQRSLGAIPFAGRYRLIDFMLS
                     NMVNADIRSVAIFPKYRYRSLMDHLGAGKEWDLHRKKDGLFFFPSPHLHHEYDEFGSFR
                     QFSDHLDYFHRSTQQYAVISNSHTVCNIQFQYVLKRHQEVGCDVTEVFQDGQSLQIYIM
                     STTLLKDLIYGHSEKGYKTIQEAVEKESSALTICPYEYSGYAAVIDSVEKYYTHSMELI
                     QPRFWQQVFLPQQPIYTKVKDEPPTKYGKHSTVKNSLVANGCVLEGEVENCILFRAVHV
                     GKGTKLKNCIIMQKTQIGEDCLLEQVISDKDVKIGKATEAAGTAEQPLVLRKGLVQGEL
                     MNS"
     regulatory      4519..4531
                     /regulatory_class="ribosome_binding_site"
                     /gene="glgD"
                     /citation=[3]
     gene            4538..5992
                     /gene="glgA"
     CDS             4538..5992
                     /gene="glgA"
                     /EC_number="2.4.1.21"
                     /function="synthesizes alpha-1,4-glucan chains using
                     ADP-glucose"
                     /note="fourth gene in glycogen operon"
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="GT5"
                     /protein_id="CAA81043.1"
                     /db_xref="GOA:P39125"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="InterPro:IPR011835"
                     /db_xref="InterPro:IPR013534"
                     /db_xref="UniProtKB/Swiss-Prot:P39125"
                     /translation="MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKYS
                     QIPEPWKKRMKKQAECTVAVGWRQQYCGIEHMAENDVNYYFIDNEYYFNRDSLYGHYDD
                     GERFAFFSRAVLEAAKVVNVQADIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIH
                     NLQFQGIFPPDVTHDLLGLEMDHFHYERLECNGFVNFMKAGIIAADHVTTVSPTYRNEI
                     MTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKL
                     QQRMGLPEKNDIPLISMVTRLTKQKGLDLVRRIMHELLEEQDIQLVVLGTGEREFEDYF
                     RYAEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRE
                     TGGLYDTVRAYQEEEGTGNGFTFSAFNAHDLKFTIERALSFYCQQDVWKSIVKTAMNAD
                     YSWGKSAKEYQRIFEQVTRSGRDVLE"
     regulatory      5965..5974
                     /regulatory_class="ribosome_binding_site"
                     /gene="glgA"
                     /citation=[3]
     gene            5979..8375
                     /gene="glgP"
     CDS             5979..8375
                     /gene="glgP"
                     /function="degrades starch and glycogen by phosphorylation"
                     /note="fifth and last gene of glycogen operon"
                     /citation=[3]
                     /codon_start=1
                     /transl_table=11
                     /product="GT35"
                     /protein_id="CAA81044.1"
                     /db_xref="GOA:P39123"
                     /db_xref="InterPro:IPR000811"
                     /db_xref="InterPro:IPR011833"
                     /db_xref="UniProtKB/Swiss-Prot:P39123"
                     /translation="MFSSKERFADLFLKRLEMTCGKSFKDSAKLDQYKTLGNMVREYIS
                     ADWIETNEKSRSNSGKQTYYLSIEFLLGQLLEQNLMNLGVRDVVEAGLKEIGINLEEIL
                     QIENDAGLGNGGLGRLAACFLDSLASLNLPGHGMGIRYKHGLFEQKIVDGHQVELPEQW
                     LKNGNVWEVRNADQAVDVPFWGEVHMTEKSGRLHFRHEQATIVTAVPYDIPIIGYETGT
                     VNTLRLWNAEPYAHYHGGNILSYKRETEAVSEFLYPDDTHDEGKILRLKQQYFLVCASL
                     KSIVNNYRKTHKSLSGLHKKVSIHINDTHPALAVPELMRILLDEENMSWEEAWHITVHT
                     ISYTNHTTLSEALEKWPIHLFKPLLPRMYMIIEEINERFCRAVWEKYPGDWKRIENMAI
                     TAHGVVKMAHLAIVGSYSVNGVAKIHSDILKEREMRDFHLLFPNRFNNKTNGIAHRRWL
                     LKANPGLSAIITEAIGDEWVKQPESLIRLEPYATDPAFIEQFQNNKSKKKQELADLIFC
                     TAGVVVNPESIFDVQVKRLHAYKRQLLNVLHIMYLYNRLKEDSGFSIYPQTFIFGAKAS
                     PSYYYAKKIIKLIHSVAEKVNYDPAVKQLIKVVFLENYRVSMAERIFPASDVSEQISTA
                     SKEASGTGNMKFMMNGALTIGTHDGANIEILERVGPDCIYTFGLKADEVLSYQENGGYR
                     SREYYQHDRRIRQVADQLINGFFEGEADEFESIFDSLLPHNDEYFVLKDFSSYADAQER
                     IQADYRERRKWSEHSIVNIAHSGYFSSDRTIREYAKDIWGIKPMM"
     stem_loop       8375..8401
                     /note="putative terminator of glycogen operon"
                     /citation=[3]
                     /function="terminator"
ORIGIN
        1 aaccgatcgg tcgcaggttc gaatcctgcc tgtggagcca aatggagaag tactcaagtg
       61 gctgaagagg cgcccctgct aagggtgtag gtcgtgtaag cggcgcgagg gttcaaatcc
      121 ctccttctcc gccatatgat tacagatatc ataattatcg gcccgttggt caagcggtta
      181 agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc atccagaagc
      241 cttgcatatc ctgcaaggtt tttttgtttt tataaatcat gatatgtctt agattttgtt
      301 ctttatttta aaaacagact acaaaaatct ccatatattt cgtttttctt cagaaaatga
      361 agttaattgt ctataagtat aagcgctttc aggaaagggc ttttttttat ttcttcgaat
      421 aaatactata aatgaaaact atgatgtcag aaaggatgat tacatggccg ctgccagccc
      481 gacagcacat gatgtttatc tttttcacga aggcagtctg tttaaaagct atcagctgtt
      541 tggctcgcat taccgcgagc tgaatggcaa aagcggatat gaattctgtg tgtgggcgcc
      601 tcatgcgtcg gaagtacgag tggctggaga ttttaacagc tggtctggag aagagcatgt
      661 gatgcacaga gtgaatgata atggcatttg gacgttattt attccaggta taggagaaaa
      721 ggaacgatat aaatacgaaa ttgtgacgaa taacggtgag atcaggctga aggcagatcc
      781 atacgctatt tattcagaag tcagaccaaa cacagcgtca cttacttacg atttggaagg
      841 atacagctgg caagatcaaa aatggcaaaa gaaacagaag gctaagacgc tgtatgaaaa
      901 acctgttttc atttatgaac tgcatcttgg gtcttggaag aagcattctg atggcaggca
      961 ttattcctat aaagaattaa gtcagacgct tatcccttat atcaagaagc atgggtttac
     1021 ccatattgaa ctgcttcccg tgtatgagca tccatatgat cgttcatggg ggtaccaagg
     1081 aacggggtat tacagcccga caagcaggtt tggcccgccg catgatctga tgaagtttgt
     1141 ggatgaatgt catcaacaaa atatcggagt cattttggat tgggtccccg gacatttttg
     1201 taaagatgca catggccttt atatgtttga tggagaaccg ctttatgaat acaaagaaga
     1261 acgtgatcgg gagaactggt tatggggcac ggcaaacttt gacctgggga aaccggaagt
     1321 tcatagtttt ttaatttcga atgcgttgta ctgggcggaa ttctatcata tagatggatt
     1381 tagggttgat gcagtggcta atatcttgta ttggccaaat caggatgaac gccacacgaa
     1441 tccatatgct gttgattttt taaaaaaact caatcaaacc atgagagagg catatcccca
     1501 tgtgatgatg attgcagaag attctacaga atggcctcaa gtgacaggtg ccgttgagga
     1561 aggggggctt ggttttcatt ataaatggaa tatgggatgg atgaatgacg tactgaaata
     1621 catggagacg ccgcccgaag aaaggcggca ttgtcatcag cttatctctt tttcattact
     1681 atatgcgttt agtgaacatt ttgtattgcc gttttctcat gatgaagtcg tatacggaaa
     1741 aaaatcactt ctcaataaaa tgcctggcga ctactggcag aaatttgccc agtatcggct
     1801 tttgctgggc tatatgacag tgcatcccgg aaagaaattg atctttatgg gatcagagtt
     1861 tgctcaattt gatgaatgga aagatacaga acagcttgat tggtttttgg actccttccc
     1921 catgcatcag aaagccagcg tttttacgca agatcttctc cgcttttacc aaaaaagcaa
     1981 aatcctttat gagcatgacc atcgtgcaca atcgtttgaa tggattgatg tccataatga
     2041 cgaacaatca atcttttctt ttattcgtta tggccaaaag cacggtgagg cgcttgtcat
     2101 catttgtaat tttacacccg tggtctatca tcaatatgat gttggagttc ctttttttac
     2161 tcaatacatt gaagtgctta acagtgacag tgaaacatat ggaggctcag ggcaaatcaa
     2221 caaaaagccg ttatcggcta aaaagggagc acttcatcat aaaccgtgtt atatcacgat
     2281 gaccatcccc ccttacggca tctcgatttt acgggcggtt aaaaaaagag gagagataaa
     2341 aagatgaaaa aacaatgtgt agccatgctc cttgccggcg ggaagggcag ccgtctcagc
     2401 ggattaacaa aaaatatggc caaaccggct gtatctttcg ggggaaaata caggattatt
     2461 gattttaccc taagcaactg ttctaattca ggcatagaca cagtcgggat tttaacgcaa
     2521 tatcagccgc tcgagctgaa ttcctatatt gggatcggca gtgcatggga cctggataga
     2581 tataatggcg gcgtgacggt tttaccccct tatgccgagt catcagaagt caaatggtat
     2641 aaagggacgg caagctccat ttatgaaaat ctcaattatt tgaatcagta tgatcctgag
     2701 tatgtactga ttttatcagg agatcacata tacaaaatgg actacggcaa aatgcttgat
     2761 tatcacattg aaaagaaagc agatgtgacc atctctgtca tcgaagtggg ctgggaggaa
     2821 gcaagccggt tcggcatcat gaaggccaat ccagatggga cgatcacaca ttttgatgaa
     2881 aagcccaaat tccctaagag caatcttgcc tcaatgggaa tttatatatt caattggccg
     2941 cttttgaaac agtatcttga gatggacgat caaaaccctt attctagcca cgacttcggc
     3001 aaagacatta ttccactgct tttggaagag aaaaagaagc tttctgctta tccgttcaag
     3061 ggatattgga aggatgtcgg aacggtgcag agcttgtggg aagccaatat ggatttgtta
     3121 aaagaggact cggagctgaa gctgtttgaa cgcaaatgga aaatttactc cgtcaatcca
     3181 aatcagcctc cgcaatttat ttcatctgat gcgcaggttc aagattcgct tgtgaacgaa
     3241 ggttgtgttg tatatggaaa tgtgtctcac tccgttcttt ttcaaggtgt gactgttggt
     3301 aagcatacga ctgttacatc atccgtcata atgccggatg tgacgattgg ggagcatgta
     3361 gtcattgaaa atgccatcgt tcctaatggg atggtgctgc cggatggcgc agtgattcgc
     3421 tccgaaaaag acattgaaga ggtgctgctc gtttcagagg aatttgtaga aaaagaattg
     3481 atttaattac tgaagagggg gcagagtcag tgttcaataa tcaaatgtta ggtgttatag
     3541 atgaaaccac atataagcat tctcttcagg atttaacagc acagcggtcg ctcggggcga
     3601 tcccttttgc cggcaggtac cgactgattg attttatgct ttccaacatg gtgaatgcgg
     3661 atattaggag tgttgcaatt tttccgaagt atcgttaccg ttcgctcatg gatcaccttg
     3721 gggcgggtaa ggaatgggat ttgcaccgga aaaaggacgg cctctttttt ttcccgtctc
     3781 cccatcttca tcacgaatac gatgaattcg gttcctttcg ccagttttcg gaccatctag
     3841 attacttcca cagaagcact cagcaatatg ctgtaatctc caacagccat accgtatgca
     3901 acattcaatt tcaatatgtg ctgaaacgcc atcaggaggt tggctgcgac gtcaccgagg
     3961 tatttcagga cggccagtcg ctgcaaatct atatcatgtc aacaacgctt ttaaaggatc
     4021 tgatctacgg acatagcgaa aaaggctata aaaccattca ggaggctgtt gaaaaggaat
     4081 cttccgccct gaccatttgc ccttatgaat attcaggtta tgccgctgtc attgattcgg
     4141 tggaaaaata ttatacccac agtatggagc tcattcagcc tcgtttttgg cagcaggtgt
     4201 ttttgccgca gcagccgatc tatacaaaag tgaaggatga gccgcctacg aaatacggaa
     4261 agcacagcac tgtcaaaaat tctttggtgg caaacggctg tgtgcttgaa ggagaggttg
     4321 aaaactgcat tttgttcagg gcggtccatg tcggaaaggg tacgaagctg aaaaactgca
     4381 ttattatgca aaaaacgcag attggagagg attgtcttct tgagcaggtc atttcggata
     4441 aggatgttaa aatcggcaag gcgacggaag ctgcaggaac agctgagcag ccgcttgtat
     4501 taagaaaagg tttggtgcaa ggagagctga tgaattcttg aagatattat ttgctgtgtc
     4561 agaatgcacc ccatttgtga aatcaggagg tcttgccgat gtagcaggtg ctctgccaaa
     4621 ggcgcttgcg cgattaggca atgaggtagc tgtgatgctg ccgaagtaca gccaaatacc
     4681 tgaaccgtgg aaaaagcgta tgaaaaaaca ggctgagtgt accgtagctg tcggctggcg
     4741 gcagcaatat tgcggaattg aacatatggc agagaatgat gtgaattatt acttcatcga
     4801 caatgagtac tatttcaaca gggattcgtt gtacgggcat tatgatgacg gtgagcggtt
     4861 tgcctttttt tcaagagctg tgctggaggc tgccaaagtt gtgaatgttc aagccgatat
     4921 tgttcatact cacgattggc atacagcgat ggtcaattat ttgctgaaag aggagtacag
     4981 aaaacatcct ttttatgaac ggatgaaaag cgtgctcacg atccacaacc tgcagtttca
     5041 aggcatattc cctcctgatg tcacacatga cttgctgggt cttgagatgg atcattttca
     5101 ttatgaaagg ttagaatgca acggatttgt taattttatg aaagcgggca tcattgcggc
     5161 tgatcatgtg acaactgtga gccccactta cagaaatgaa atcatgacgc cgtattatgg
     5221 tgagcagttg gaacaggttt tgcaatatag ggaagatgat gtaaccggca tattaaatgg
     5281 cattgatgat acgttctatc agccgaaaag cgacccttat atagaggcgc aatacgattc
     5341 aggggatctt gcatgcaagc tggaaaacaa aacgaagctt cagcagcgga tggggcttcc
     5401 agagaaaaac gatattccgc tgatcagtat ggtgacaagg ctcactaaac aaaaggggct
     5461 tgatcttgtc cgcagaatta tgcacgaact tcttgaggag caagacatac agctggttgt
     5521 gctaggcaca ggagaacgtg aatttgaaga ttatttccgg tatgcagaat ttgcttttca
     5581 tgagaagtgc cgggcttata tcgggtttga tgagccgcta gcccatcaaa tttatgcggg
     5641 ctctgatatg tttttgatgc cgtcgaaatt tgagccgtgc ggtttggggc agctcattgc
     5701 tcttcaatat ggcgccattc cgattgtcag ggaaacgggc ggtctttacg acactgtccg
     5761 tgcctatcag gaggaagagg gaacgggcaa tggctttacg ttttccgcct ttaatgcgca
     5821 tgatctgaaa tttacgatag aaagggcgct gtcgttttat tgccaacagg atgtatggaa
     5881 gagcatcgtg aagaccgcta tgaatgcaga ttacagctgg gggaagtcgg ctaaagagta
     5941 tcagcgtatt ttcgagcagg tgacaaggag tggacgggat gttctcgagt aaagaacgct
     6001 ttgcagactt atttttaaag cgtctggaaa tgacgtgcgg aaaaagcttt aaagattctg
     6061 ccaagctgga tcaatataaa acgttgggca atatggtcag agagtatatc agcgctgatt
     6121 ggatcgaaac gaatgaaaaa agcagatcaa attccgggaa gcagacatat tatttatcaa
     6181 tcgaatttct gctcggtcag ctccttgagc aaaacttaat gaatcttggt gttcgtgatg
     6241 ttgtagaagc tggtttaaag gaaatcggca tcaatttaga ggagatcctt cagatagaga
     6301 acgatgcagg tctcggcaat ggcggacttg ggcgcttggc cgcttgcttt ttagattcgc
     6361 ttgcttcgtt gaacctgcct gggcacggca tgggcatacg ttataagcat ggtttgtttg
     6421 agcaaaaaat tgtagacgga catcaggtgg agctgcctga gcaatggctg aaaaacggga
     6481 acgtatggga agtaaggaat gcggatcaag cagtggacgt gccgttttgg ggcgaagtcc
     6541 atatgacaga aaaaagcggg cgcctgcatt ttcgccatga acaagcgaca attgttaccg
     6601 ctgttcctta tgatatcccg atcatcggct atgaaacggg cactgtcaac acgttaaggc
     6661 tttggaacgc cgagccctat gcccattatc acggcggtaa tattttgtct tataagcgag
     6721 aaacagaagc ggtttctgaa ttcttatatc ccgatgacac ccatgatgag gggaaaattt
     6781 tacggctgaa gcagcagtat tttttagtat gcgcaagctt aaaaagcatt gtgaacaatt
     6841 accgtaaaac acataaatct ttatccggcc tgcataaaaa agtgagcatt catattaatg
     6901 acactcaccc tgcgcttgcg gtgcctgagc taatgcggat tttgctcgat gaagagaaca
     6961 tgagctggga agaagcttgg catatcactg tacatacgat ttcttataca aatcatacaa
     7021 cattgtcaga agcgctcgag aaatggccga ttcatttgtt taagccgctg ctcccgcgaa
     7081 tgtacatgat tattgaagag atcaatgaga ggttttgccg tgcagtttgg gaaaagtatc
     7141 ctggagactg gaaaaggata gagaacatgg cgattacagc gcatggtgtt gtcaaaatgg
     7201 ctcatctggc catagtaggc agctacagtg taaatggcgt tgcaaaaata cattccgata
     7261 tattaaagga aagggagatg cgcgactttc acttgctgtt tccgaatcgt tttaacaata
     7321 agacgaatgg aattgcccac aggcgctggc tgttaaaagc gaatccaggc ttgtctgcca
     7381 tcattacaga ggcgatcggc gatgaatggg tcaagcagcc ggaatcactg atccgtttag
     7441 aaccgtacgc cactgatcct gcatttattg aacagtttca aaacaacaaa agcaagaaaa
     7501 aacaagagct tgccgatttg attttttgca cggccggggt ggttgtgaac cctgagagca
     7561 tattcgacgt acaggtgaaa cggcttcatg catataaacg gcagctgctg aatgttcttc
     7621 acattatgta tttgtataac cgcctcaagg aggattcggg gttttcaatc tacccgcaaa
     7681 cctttatttt cggggcaaag gcttcaccaa gctattatta tgccaagaaa atcattaagc
     7741 tgatccattc tgtcgcagaa aaagtcaatt atgatccggc ggtcaaacag ctgatcaaag
     7801 ttgttttttt agaaaattat agagtttcta tggctgagcg aatttttccg gcatcagacg
     7861 taagtgagca aatttcaacc gcaagcaagg aagcgtctgg tacagggaat atgaagttta
     7921 tgatgaacgg ggctttgacg atcggcacgc atgacggcgc aaatatcgag atccttgagc
     7981 gggtgggccc tgactgtatt tacacgttcg gcttaaaggc ggatgaggtt ctatcgtatc
     8041 aggaaaacgg cggctatagg tcgagggaat attatcagca tgacaggaga atcaggcagg
     8101 tagcggatca gttgatcaat ggtttttttg aaggagaggc agatgaattt gaatcgatct
     8161 ttgactcgct gcttccgcat aatgatgagt attttgtgct gaaggatttc agctcatatg
     8221 cagatgcaca ggagcgaatt caagctgatt atcgagagcg aaggaaatgg tctgagcatt
     8281 cgattgtaaa cattgctcat tctggatatt tctccagcga ccgcacgatc cgggaatatg
     8341 cgaaggacat ttggggcatc aagccgatga tgtgaaaaag ccgctgtata agcggctttt
     8401 tttgttcaca gattcattga attgattgta aaggataaga acgtcgcaaa tgcactccat
     8461 aaaaaataag gcaaaagaag atagcttgcg gctctgctgt attttttggc aatgatc
//
