LOCUS       NZ_DS995511            11090 bp    DNA     linear   CON 20-DEC-2019
DEFINITION  Bacteroides eggerthii DSM 20697 Scfld3, whole genome shotgun
            sequence.
ACCESSION   NZ_DS995511
VERSION     NZ_DS995511.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00000667
            Assembly: GCF_000155815.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacteroides eggerthii DSM 20697
  ORGANISM  Bacteroides eggerthii DSM 20697
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides eggerthii (DSM 20697)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides eggerthii (DSM 20697)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11090)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2008) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   4  (bases 1 to 11090)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2008) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/16/2019 03:49:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 3,453
            CDSs (total)                      :: 3,392
            Genes (coding)                    :: 3,323
            CDSs (with protein)               :: 3,323
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 5 of 69
            Pseudo Genes (frameshifted)       :: 36 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 15 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            DS995511.
            Bacteroides eggerthii (GenBank Accession Number for 16S rDNA gene:
            L16485) is a member of the Bacteroidetes division of the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from Deutsche Sammlung von Mikroorganismen und
            Zellkulturen GmbH (DSMZ) (DSM 20697).
            This is a Newbler assembly
            (http://www.454.com/enabling-technology/the-software.asp)
            consisting of 2 full plate runs of a fragment library and 2 full
            plate runs of a paired end library with a Q20 coverage of 60.8X.
            This sequenced strain is part of a comprehensive,sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Bacteroides eggerthii (GenBank Accession Number for 16S rDNA gene:
            L16485) is a member of the Bacteroidetes division of the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from Deutsche Sammlung von Mikroorganismen und
            Zellkulturen GmbH (DSMZ) (DSM 20697).This is a Newbler assembly
            (http://www.454.com/enabling-technology/the-software.asp)
            consisting of 2 full plate runs of a fragment library and 2 full
            plate runs of a paired end library with a Q20 coverage of 60.8X.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut  microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.0. Gene names are generated at the contig level and may not
            necessarily reflect any known order or orientation between contigs.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Annotation was added to the contigs in December 2008.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Bacteroides eggerthii DSM 20697"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld3"
                     /strain="DSM 20697"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Bacteroides eggerthii"
                     /db_xref="taxon:483216"
     gene            complement(1..2271)
                     /locus_tag="BACEGG_RS14715"
                     /old_locus_tag="BACEGG_03245"
     CDS             complement(1..2271)
                     /locus_tag="BACEGG_RS14715"
                     /old_locus_tag="BACEGG_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL6_1|PL6"
                     /protein_id="WP_004291728.1"
                     /translation="MLKSFIKLCWIFPLLAACSQGNWTIDLNSLQNTLEKARPGDTIYI
                     KSGTYTNIQLQLEGYGTVEEPIVVMTQQPGSVFIEGVSNLRLCGEYVEINGLHFRNGYT
                     PKGAVIEFRKGEKVANNCRITDCVIDYFNPIDRGVSGSWILLYGRNNRLDHNSILGKLY
                     AGVTLAVILNGERDRNNNHRIDHNYFGERPILGSNGGETIRVGTSHHAFFSSNTVIEDN
                     MFHHCNGEVEVVSIKSSDNIIRNNVFLECRGILALRHGNRNLVEGNAFIGNGLPCTGGV
                     RVVNEGHIIKGNLFYGLKGDRFFAALGLMNAVPNSLPNRYHHVKDVTLEDNRFINCDNI
                     LFCVGKDNERTLPPSNISFIRNQFISKSDKALYQSFDDISGFTFIDNVVNYPYTVTQRG
                     FQNNTTLLDSIDLKPYMEKKLGASWYTFSENHKLMLTGNEISVKAGQNTLLEAFNKAQS
                     GDILNLSEEGVYWLDNTLLIDKYIRIQADSHLSKRPVLCFNGMSGKAFVTIVNGGNLEI
                     QGLAFNGEGEAGKALSEGGITVKSGTITPYLLTVDNCEFYNFNESGLAAIRGEKSTFSP
                     MVIIRNSFFHDMSGEAINFAGEKDDKGKYNVEELHVGNCIFYRLLGSALNIYRGGNDES
                     TSGPLLTVNHCTIENVDNKEQGSAMRLIGVQSATVTNCSFANSGKGGASIRFNEMSWDK
                     LSVSYINLYNSGCIASFWGKLGSKSITNYKPEYVDVNTGNFYQTSTSPLRNRASDKKDL
                     GIT"
     gene            complement(2302..3882)
                     /locus_tag="BACEGG_RS14720"
                     /old_locus_tag="BACEGG_03246"
     CDS             complement(2302..3882)
                     /locus_tag="BACEGG_RS14720"
                     /old_locus_tag="BACEGG_03246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="WP_004291730.1"
                     /translation="MMKRGCIFVCLQICICWGLLWGVSTGNNFQLSLAEVFGEGMVLQQ
                     EDINNIWGIGRPFAIVQVEFQNQKVKTEVKPDGRWMLSLKNLAAGGPYRLKVTSGNESV
                     LLSEVYVGEVWLATGQSNMERKLFMSENGEAVVRSANNQNIHFLIVPQVYYKGHNVNKE
                     MKWQTATAPQVANMSAIGYYFACKLQKELNVPIGIICCYRGGTPAEAWVKEETLERDDK
                     LRPILDNYRKVAIMDDTLYEKKMEEYKVLYRVYNDSVTMGYKNVVRPFEPVGPKHHKRP
                     CGLYHTMLQRIIPYTAKGVIWYQGEGNAERAEQYRVLFPALIRQWRTDFHQPDLPFYFV
                     QLSNFDHPFWQDRPNWAELRDAQLFTWKTVKNTAMVVSIDKGEKNDVHPIYKKPIGERL
                     ASCALHLQYDKKVPYSGPVYKSMQIKDNKAEISFDFVYSGLTALGEELKGFSICGADYN
                     FVSARAEIRDGKVFVWSDVVERPIAVRYGWSNWTEANLFNKEGFPATPFRTDNFSLMTE
                     GIYYPMKIR"
     gene            complement(3888..5345)
                     /locus_tag="BACEGG_RS14725"
                     /old_locus_tag="BACEGG_03247"
     CDS             complement(3888..5345)
                     /locus_tag="BACEGG_RS14725"
                     /old_locus_tag="BACEGG_03247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_004291732.1"
                     /translation="MLTDMKKMKYIIGLGLSLFLASCTDALDLQPKSDLTQTNFFNKTS
                     DVELALVGCYDGLQQMSLNHDYVLMEMRSDLSSSYLAEGEFGYIDTFVKDIVESGDVYN
                     YWKACFNALSRTNLVIDNLGIVNDEVTRNRIEGEARFIRALVYFNMVRLWGDLPIITRT
                     IDANETDMFRRKPLSEVYSTVIIPDLEFAINNLPDMTKGKANAMAAKALLAKVYLTLGR
                     HSDAKTLLATLINMSDPSKSTVSGVGLASDYSSIFSESTKWNSEILFAVQFNSTSGEGQ
                     TFSYDFSVFGGNSGLNTPTTPFAEECESDVVRSQTNLMEEDKAGIWIVCGKYLKRGTKP
                     SQKDNAADAPILRYADVLLMYVEAVLGAPSEPYANDITNDVNALAVYNAVRARAGHLPV
                     TSVSRNELLQERKFEFAFENQRWFDLQRVVGVSETIRIMEAHGQDSRLLPKQKFTKVER
                     FQMLYPIPGREIRLLGKEILSQNEGYN"
     gene            complement(5352..8393)
                     /locus_tag="BACEGG_RS14730"
                     /old_locus_tag="BACEGG_03248"
     CDS             complement(5352..8393)
                     /locus_tag="BACEGG_RS14730"
                     /old_locus_tag="BACEGG_03248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="WP_115615987.1"
                     /translation="MQLYGMAIFAQTKIVTGQVIDVTHSPLPGVNILVKGTTIGCITDI
                     DGNYSISVPDLEADILVFSYIGFTQKEVACKGQPKINVTLTEDQQQLDEVVVVGYGVQK
                     KSHLTGSISKIKNENLGQLPVSRADQALVGKLAGVQIQNVSAQSGASPKIQIRGIGSIS
                     ADTNPLIVVDGYPIPGDLSDVDMNDVESIEVLKDAASAAIYGSRGANGIIIVTTRSGSS
                     EGSKPTIRINSYGGVKERLLKGIQRTRPEDWKIKAEQIYAEMGRPTSGAHASMVDDMKK
                     VYVASDVLGTQTDWVEETIKLGYIQNHQISVSGGSKNVNYYASGSFLDEKGIVITDRYK
                     KFNLNAKVDAKINDKLEFGVNVNAVVDSQRAYRSGSQSNINQVFRDAMYIPVRHTAETI
                     EWARLSDPTLNIGDYAHEYHFSKVPYNGEYISVKNAGGNNGISTTLERNDRTGYIKALG
                     NIYLQYQPVKGLILKSSFGGYASMMDYKFYQSSLAHNNGKTSGQYKTAKVIDWLNENIA
                     TYTPNLGENHSLSILAGYTVQKTTGYNSNMTASDFPTDNIQTLNAGIINSGNTTEYEEL
                     LLSALGRVTYSYKGKYLASVSSRWDGSSRFGINNKWGYFPSVSLGWRISEESFFTPVKK
                     IIDELKIRASFGASGNKNIGNYQAFGLLSQSNAVINNKVTPGYTQISSENKDLGWERTY
                     QYNGGFDLGMFNNRLRINLDAYYTITDKLLLEREISSMTGQTNQLVNMGKMSNTGYEIE
                     ITTQNIQTKDFSWSTSLNIAQNFNKVLNLGGVDKIISSPKGEEKRPTYFVTEVGKPLVQ
                     FYGFVVEKEIPNEGLISPVWPVDVKPELVHVKDLDGNGKIDDDDMVAQGSPYPKVTWGM
                     TNDLKWKDFDLNVVVQGSHGNKVFNIDEHYAQSQWNGKLLPEYQNSYNNIKHKASACWF
                     VEDASFIAIRSINLGYTLPKNIMKNCSLRVYASIYNPCYFLLGDYKGYNPEGIKSFDTP
                     LIDGYQSGAMPLARTYTLGFNFQF"
     gene            8928..11090
                     /locus_tag="BACEGG_RS14735"
                     /old_locus_tag="BACEGG_03249"
     CDS             8928..11090
                     /locus_tag="BACEGG_RS14735"
                     /old_locus_tag="BACEGG_03249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL17|PL17_2"
                     /protein_id="WP_004291737.1"
                     /translation="MKTKSILTTIFLFLAFTIQAKLPEHPCLLLTQKGIEKIKAQNKPA
                     MLFNTTLTQIKNQIYPILKQPIEVPLPKDAGGGYTHEQHKRNYNNMYNAGVLFQLTEKK
                     EYAEYVKKMLIQYAEMYPNLPLHPIQKSNYRGKLFWQGLNECVWLVYTSQAYDCIYNYL
                     NPKEREFIENNLFKPMVEFIAIHNKSTFEKIHNHATWAVTGVGMISYVMGDKDMLEKAL
                     YGLDKSGKSGFIRQIDQLFSPDGYFEEGPYYQRYSLQPFIMFAQAIENNEPERKIFEYK
                     NGVILKAVTTLLQMSENNGQFFHFNDALDKTWHSTELVWGIDISYNRTGNKQLLSIAKE
                     QNTVILNEAGYKVATDIELAQPFIHQTMVISDGAKGDEGGIGILRCPNKNNESCVALKY
                     TSQGMGHGHFDRLSFTYYDNNKEIIQDYGAARFLNIEPKNGGHYLPENNSFSKQTIGHN
                     TLIVDETSHFNGKLDEASKYSPTFYAFINGEQVKMISAKDKHAVPGVTMQRNLLLISHP
                     AFEHPLIIDIFKIISQKKHQYDLPYYYKGHLINTDYKYNAFDTTRTLLGSKNGYQHLWI
                     EAQGNPRQETTSTTWLNDNRFYTLTAVTDENTEIFLTRIGGNDPNFNLRNDPCLMFRQK
                     ESDNHTFVSLIEAHGEYNPRLEYTLAPYTNVQNIQIIMDTEEYTILKVTTKNKQAITIC
                     FANTDNNPHSVHKIRDYSWQGVCEIK"
ORIGIN
        1 ttatgtgatt cctaaatctt tcttgtcaga agctctattt cttaatggtg atgttgaagt
       61 ctgataaaaa ttaccagtat tcacatctac atattcaggt ttatagtttg taatactctt
      121 actccccaat ttaccccaaa aagaagcaat acacccagaa ttatacagat taatataaga
      181 tactgacaat ttatcccaac tcatctcatt gaaacgaata ctggctcctc cctttccact
      241 gttggcaaaa gaacaattag tcacagtagc actttgtact cctatcaaac gcatagcaga
      301 accttgttct ttattgtcca cattttcaat tgtacaatgg ttaactgtca gcaatggtcc
      361 actggtgctt tcatcatttc cacctcgata aatattcaaa gcactaccta ataatcgata
      421 aaatatacaa ttaccaacat gtaactcttc tacattgtat ttacctttat catctttctc
      481 tccagcaaaa ttaattgctt cccctgacat gtcatgaaaa aaagaattcc ggataatcac
      541 cattggtgag aaagtagatt tttcgccacg aatggcagcc aagccagatt cattgaaatt
      601 atagaattcg caattatcaa cagtaagtaa ataaggagta atagtacctg atttcacagt
      661 tataccacct tcagacaaag ctttccctgc ctcaccttca ccattgaaag caagaccttg
      721 tatttccaag tttccaccat tgacaatggt cacaaatgct ttaccagaca taccattaaa
      781 acataaaacg ggacgtttag ataaatggga gtctgcttga atacgaatat atttatctat
      841 caacaaagta ttatccagcc aataaacacc ctcttccgag agattcaaaa tatcaccgga
      901 ctgcgctttg ttaaaagcct ctaatagcgt attttgtcct gccttcacac tgatttcatt
      961 accagtaagc atcaatttat gattttcaga aaaagtatac caacttgcac cgagtttttt
     1021 ctccatatat ggctttaaat caatactatc taataaagtc gtattattct gaaaacccct
     1081 ttgtgttacg gtatagggat aattcaccac attatcaata aaagtaaagc cagaaatatc
     1141 atcaaaagac tgataaagcg ctttatctga cttactaatg aactgattac ggataaaaga
     1201 gatatttgag ggtggtaatg tacgttcatt atcttttcct acacaaaata ggatattatc
     1261 acaattaata aaacgattat cttctaacgt tacatccttt acatgatgat aacggttagg
     1321 taaagaatta ggaaccgcat tcatcaaccc aagagccgca aaaaaacgat cacctttcag
     1381 cccatagaac aaattcccct taataatatg gccttcattg actacacgta ctccgcctgt
     1441 acaaggtaat ccatttccta taaatgcgtt tccttcaact aaattacggt taccatgacg
     1501 taaagccaaa ataccacgac attctaaaaa tacattatta cgaataatgt tatcacttga
     1561 tttgatagaa acaacttcaa cttccccatt acaatggtga aacatattat cttctatcac
     1621 tgtattcgaa gaaaaaaatg catgatgtga ggttcccaca cgaatcgttt cgcccccatt
     1681 tgagcccaat atcgggcgtt caccgaaata attgtgatca attctatgat tattattacg
     1741 gtctctctca ccatttaaaa taacagccaa agttactcct gcataaagct tacccaaaat
     1801 agaattatgg tccagtcgat tatttcgtcc gtaaagaaga atccagctcc cagatacccc
     1861 acgatctatc ggattgaagt aatcaatgac acaatctgtt atacggcagt tgttagcaac
     1921 tttttcacct ttacgaaact caatcacagc tcctttagga gtgtaaccat tacgaaaatg
     1981 caatccgttt atttcaacgt attctccaca taaacgaaga ttagatacac cttctataaa
     2041 aacactgcct ggttgttgag tcataacaac aatcggctct tctaccgtac catacccctc
     2101 gagttgtaac tggatatttg tatatgtacc tgatttaata taaatcgtat cacctggacg
     2161 agccttctcc aaagtatttt gaagagaatt taagtcaata gtccaatttc cttgcgaaca
     2221 tgcggccaac aaaggaaaaa tccaacaaag tttaataaat gatttaagca tcacaagcta
     2281 tatatattaa agtttcacta gttaacgaat tttcatcgga taataaatac cctctgtcat
     2341 taatgagaaa ttatctgtac ggaaaggagt tgctgggaaa ccctctttat taaaaagatt
     2401 agcttccgtc caattagacc aaccataacg taccgcaata ggacgctcta caacatcaga
     2461 ccagacaaat acttttccat ccctaatttc cgctcttgcc gatacaaaat tataatccgc
     2521 cccacaaata gaaaaacctt tcaattcctc gcccaaagca gtcaaaccag aataaacaaa
     2581 gtcaaaacta atttctgctt tattgtcttt tatttgcatt gatttatata ccgggcctga
     2641 atatggtact tttttatcat attgcaaatg caaagcacag gatgctaaac gttcaccaat
     2701 aggtttctta taaatagggt gtacatcgtt tttctcccct ttatcaatac tgactaccat
     2761 agcagtattc tttactgttt tccatgtaaa tagctgggca tcacgtaact cagcccaatt
     2821 tggacgatct tgccaaaaag gatgatcaaa attcgacaac tgaacaaaat aaaatggtaa
     2881 atcaggttga tgaaaatcag tacgccattg tctaattaat gctggaaaaa gtactctata
     2941 ctgctccgcc cgctctgcat taccctcgcc ttgataccaa attacacctt tagccgtata
     3001 aggaataata cgctgcaaca tagtatgata caatccacaa gggcgtttat gatgtttagg
     3061 gcccactggt tcgaaaggac gaacaacgtt tttatatccc atagtcactg agtcattata
     3121 aaccctataa agtaccttat actcctccat ttttttctca tataaggtat catccataat
     3181 agcaacttta cgataattat ccaaaatagg acgtaattta tcatctcttt ccagcgtctc
     3241 ttctttcacc caagcttcag caggcgttcc accacgataa caacaaatga taccaatagg
     3301 aacatttaat tctttctgaa gtttacaagc aaaataataa ccgatggcag acatattcgc
     3361 aacttgtggc gctgttgcgg tctgccattt catttcttta ttaacattat gccctttata
     3421 atatacttga ggtactatta gaaaatgaat attctgatta ttagctgaac gaacaacagc
     3481 ttctccattt tcactcataa acagcttacg ctccatattt gattgtcctg ttgctaacca
     3541 cacttctcca acatacactt cagacagtag aacagattca tttcctgaag ttacttttaa
     3601 ccgatatggc cccccagcag ccagattctt caaagacaac atccagcgcc cgtcaggctt
     3661 aacttctgtc tttacttttt gattttgaaa ttcgacctgt actatagcaa aaggacgtcc
     3721 tattccccaa atattattta tatcttcttg ctgcaatacc attccttcac caaaaacttc
     3781 agctaaagac aactggaaat tattacccgt ggagactccc caaaggagcc cccaacagat
     3841 acaaatttgc aaacatacaa atatacaacc tcttttcatc atttttatca gttataaccc
     3901 tcgttctgag ataaaatttc ttttcctaac aaacgaattt cacgtcccgg aataggataa
     3961 agcatttgaa atctttctac cttagtaaat ttctgctttg gtaaaagtct agagtcctgt
     4021 ccgtgtgctt ccataattcg aatcgtttca gatactccaa ctacacgctg taaatcaaac
     4081 caacgttggt tttcgaacgc aaactcaaat ttacgttcct gaagtaattc atttctagaa
     4141 accgaagtta cgggaagatg ccctgcacgt gccctcactg cattataaac ggctaaagca
     4201 ttcacatcat tagttatatc attcgcataa ggttcagagg gagcacctaa taccgcttca
     4261 acatacatta aaagtacatc agcataacgt aaaataggag catcggcagc attatccttt
     4321 tgtgaaggct tagttcccct tttcaagtat ttaccacaca caatccaaat acccgcctta
     4381 tcttcttcca ttaaattggt ctggctacgt acaacatcac tttcacattc ctcagcgaat
     4441 ggagtagtag gtgtatttaa acctgaatta ccaccaaata cagaaaaatc ataactaaat
     4501 gtttgtcctt cacccgaagt agaattaaat tgaacagcaa ataatatctc actattccat
     4561 ttagtgcttt ctgagaatat agagctatag tctgaagcta atcctactcc tgaaacagta
     4621 ctcttgctag gatctgacat attaatcaac gtagcaagca aagtctttgc atcggaatga
     4681 cgacctaaag taagatatac tttagcaagc aacgctttag ctgccatagc atttgctttg
     4741 cccttagtca tgtcaggcaa attatttata gcaaactcta aatcgggaat aatgacagta
     4801 gaataaacct cagaaagagg cttccggcga aacatatctg tttcattggc atctatcgta
     4861 cgagtaataa taggtaaatc cccccataaa cgaaccatat taaagtaaac taatgcacgt
     4921 ataaagcgtg cttcaccttc tattcggttg cgagtcacct catcatttac tatacccaaa
     4981 ttatctatta ctaagtttgt acgggaaaga gcattgaaac aggctttcca ataattatat
     5041 acatcaccac tctcaacaat atctttcaca aaagtgtcaa tatatccaaa ctcaccttct
     5101 gccaaataag aagaagaaag atctgaacgc atttccatca atacataatc atgattcaaa
     5161 gacatttgtt ggagaccgtc ataacaacca accaaagcca attcaacatc acttgtctta
     5221 ttaaagaagt ttgtttgtgt caaatctgat ttaggttgta aatccaatgc atccgtacag
     5281 gaagccaaaa ataagcttaa acccaaacct attatatatt tcattttttt catatcagtc
     5341 aacattagta attaaaattg aaaattaaag ccaagcgtat aagtacgagc caacggcatt
     5401 gcaccactct gataaccatc aatcaatgga gtatcaaagc tcttaatacc ttcaggatta
     5461 taccctttat aatcacccaa caagaaatag catggattat aaatagacgc ataaacacgt
     5521 aatgaacagt tcttcataat atttttaggc aatgtatatc ctaaattgat actgcgtatt
     5581 gcaataaaag aagcatcctc tacaaaccag caggctgaag ctttatgttt gatattatta
     5641 taggaattct ggtactctgg caacaatttc ccattccatt gactctgagc ataatgctca
     5701 tcaatattaa atactttatt accatgtgac ccttgaacca ctacatttaa atcgaaatct
     5761 ttccatttta aatcattagt cataccccaa gttactttcg gatatggaga gccctgtgct
     5821 accatatcat catcgtctat ttttccattg ccgtccaagt ctttaacatg taccaattct
     5881 ggtttcacat caaccggcca tacaggactg ataagccctt cgttaggaat ctctttttca
     5941 acaacaaagc catagaattg aactaaaggt ttaccgacct cagttacaaa atatgtagga
     6001 cgtttttctt ctcctttagg actgcttata atcttatcta caccacctaa gtttaaaacc
     6061 ttattaaagt tctgtgcaat atttaaactt gtagaccaag agaaatcctt tgtttgaata
     6121 ttctgggttg taatttcaat ttcataaccg gtattactca ttttacccat attaaccaac
     6181 tggtttgttt gtcccgtcat actagagatc tctctttcca aaagtagttt atccgttatc
     6241 gtatagtaag catcaagatt aattctcaaa cgattattaa acatacccaa atcaaaacca
     6301 ccattatact gataagttct ctcccaaccc aagtctttat tttcactgga aatttgtgta
     6361 tatccaggag ttactttatt attgattaca gcattggact gtgaaagaag accaaatgct
     6421 tggtaattac caatattttt attaccactg gcaccaaaac ttgctctaat ctttaattca
     6481 tcaattatct tcttaaccgg agtaaagaat gattcctcag aaatacgcca acctaaagat
     6541 actgaaggga aataccccca tttattatta ataccgaaac gagaacttcc atcccaacgt
     6601 gaacttacag aagcaagata tttaccttta taagaatatg tgacacgtcc tagtgccgat
     6661 aatagcagtt cctcatactc agtagtatta cccgagttaa tgataccagc attcaaggtt
     6721 tgaatattat cagtcggaaa gtctgaagct gtcatattgc tattatagcc tgtagtcttt
     6781 tgaactgtat aaccagccaa tatacttaaa gaatgatttt ctcctaaatt aggagtatag
     6841 gtcgcaatat tttcattcaa ccaatctatt acttttgcag ttttatattg tccgctcgtt
     6901 ttaccattat tatgagctaa actactctga taaaatttat agtccatcat agaagcatat
     6961 ccaccaaaag aactttttaa aatcaaacct ttaacaggtt gatactgtaa gtagatattt
     7021 cctaaggctt taatataacc agttctatca ttacgctcta aagtagtaga aataccatta
     7081 ttaccaccag cattttttac actaatatat tctccattat aagggacttt cgaaaaatga
     7141 tattcatgag catagtcacc tatatttaat gtaggatcag acaaacgagc ccattcaatt
     7201 gtctcagcag tatgccttac tgggatgtac atagcatctc taaatacctg attaatattt
     7261 gattgagatc ctgaacgata agcgcgttgg gaatcaacaa ctgcattcac attgacgccg
     7321 aattctaatt tatcattaat cttagcatca accttagcat ttaaattaaa ctttttataa
     7381 cgatctgtta taacaattcc tttctcatcc aaaaaagaac cagatgcgta gtagttcacg
     7441 ttttttgaac cgccagaaac actaatctga tggttctgta tatatcccag ttttattgtt
     7501 tcttctaccc agtcagtttg agttcccaaa acatcagaag caacataaac ttttttcata
     7561 tcatctacca tagacgcatg agctccactg gtcggtctac ccatttctgc atatatttgt
     7621 tctgctttta tcttccaatc ttcaggacgt gtacgttgta ttcctttcaa cagtcgctct
     7681 ttaacaccac cataactatt tatgcgaata gtaggtttag agccttcaga gctaccacta
     7741 cgtgtagtca caataataat accatttgca cctcttgaac cataaatagc agctgatgca
     7801 gcatctttca atacctcaat agattctaca tcattcatat caacatctga caaatctcct
     7861 gggataggat aaccgtctac tacgatcaga ggattcgtat ctgcagaaat agacccaata
     7921 ccacgtatct gaattttagg actagctccc gattgcgctg aaacattttg aatttgcaca
     7981 cccgccaatt taccaactaa cgcctggtct gcacgagaaa cgggcaattg ccccagattc
     8041 tcatttttaa tttttgaaat agaacctgtt aaatgagatt tcttctgaac accataacca
     8101 accactacta cttcatctag ctgttgttga tcctcagtca aagttacatt tatttttggc
     8161 tgtcctttgc aagctacttc tttctgagta aacccgatat atgaaaatac aagaatgtct
     8221 gcttctaaat ctggtacact aattgagtaa tttccatcaa tatcagtaat acatccaata
     8281 gtagtcccct ttacaagaat attcacacca ggcaaaggcg aatgtgtcac atctataact
     8341 tggcctgtca caattttcgt ttgggcaaaa atcgccatac catacagttg catccagcaa
     8401 aacatgatta acaaaaaagt tcgccgagaa gcagtttgaa ataattgtct gttcttcata
     8461 aaactataaa attaatgatt aacaataaat atatatcaca ataagataat aatcctaata
     8521 gtagttttac catcttccat agccattcgc tacagaaaag ttgatcactg cacaaattgg
     8581 tttgccaaaa ataatagtat ttttaataat atcaaagtaa aaagaagaaa aatataaaat
     8641 aaatcatatt accacataag ggttctataa ataacgatat acacataaaa tagtacgcgt
     8701 acattattat atcattaagc aaaaatcaat ataatatact aataatcaac atatttatat
     8761 ttaaacaaag aaagcaaaag aagaaaggga acaatcataa aaagtgcgca caaaaaaata
     8821 ttaggtgaac ctatgataaa aaacataaaa aatatctggt attttatttg ctcaaaataa
     8881 aattaaccac tatttttgga aaaccaattt aaaacaaaga aatactcatg aaaacaaaaa
     8941 gtattctaac aactattttc ttatttctgg catttacaat ccaggccaag ttaccagagc
     9001 atccctgctt attgcttact caaaaaggaa ttgaaaaaat aaaagctcaa aataagccgg
     9061 ctatgctatt caatacgaca ttaactcaaa taaaaaatca aatatatcct atcttaaaac
     9121 aaccgataga agttccactt cctaaagacg caggaggtgg ctatacacac gaacaacata
     9181 agcgcaacta taataacatg tataatgcag gtgtactttt ccaattaact gaaaaaaaag
     9241 aatatgcgga atatgtaaaa aaaatgctta tacaatatgc agaaatgtat cctaatctac
     9301 ccttacatcc aattcaaaaa tctaattata gaggaaagct cttttggcaa gggttaaatg
     9361 aatgtgtttg gttagtatac acatctcaag cctatgattg tatatataat tacttaaatc
     9421 ccaaagaaag agagtttatt gaaaataatt tatttaagcc aatggttgaa tttatcgcta
     9481 tacacaataa atccacattc gaaaaaattc acaaccacgc tacctgggca gtaacaggag
     9541 tggggatgat ttcctatgtt atgggagata aagatatgct agagaaagca ttatatggac
     9601 tagataaaag cggaaaaagt ggttttatta ggcaaataga tcaacttttt tcacccgacg
     9661 gatatttcga ggaagggcct tattatcaga gatattcatt acagccattc attatgtttg
     9721 ctcaagctat tgagaacaat gagcctgaaa gaaaaatatt tgaatataaa aatggagtaa
     9781 tattaaaagc cgtcactacc ctattacaaa tgtctgaaaa caacggccaa ttctttcatt
     9841 tcaatgacgc tttagacaag acctggcaca gtacagagtt agtttggggt attgatatct
     9901 cctataatag aacaggcaac aaacaactat tatctatagc aaaagaacaa aatactgtta
     9961 tcctaaatga agctggatat aaagttgcta ctgacataga acttgcacaa ccttttatac
    10021 atcaaacaat ggtcataagc gatggagcca aaggcgacga gggtggcatt ggaattctac
    10081 gctgcccaaa taaaaataat gaaagctgtg tagctctaaa atacacatca cagggtatgg
    10141 gacatggaca ctttgatagg ttatcattta cctattacga caataataaa gaaataatac
    10201 aagactacgg ggctgcacgt tttttaaata tagagcctaa aaatggcggc cattatttac
    10261 ctgaaaataa tagctttagt aagcaaacaa tagggcataa caccctaata gtcgacgaaa
    10321 ccagccactt caatggtaaa ttggacgaag catcaaaata ctctcccaca ttctatgctt
    10381 tcatcaatgg cgaacaggta aaaatgatca gtgcaaaaga caaacacgct gttcccggag
    10441 taactatgca gcgaaatcta ctgctaatct cccatcctgc ttttgaacat cctctcatta
    10501 ttgatatatt caaaataatt tcacaaaaaa aacatcaata cgatttgcct tattattata
    10561 aagggcatct cattaatact gattataaat acaacgcatt cgatacaact cgcactttgt
    10621 taggcagcaa aaacggatac caacatttat ggatagaagc acaaggaaac cccaggcaag
    10681 agacaacttc taccacttgg ttaaacgata atagattcta tacattaacc gctgtaaccg
    10741 atgaaaatac agagatattt ctaaccagaa ttggtggtaa cgaccctaac ttcaatttga
    10801 gaaacgatcc ttgcttaatg tttcgccaga aagagagtga taatcataca ttcgtatctc
    10861 tcatagaagc acacggagaa tacaacccac gtctggaata tacattagct ccttacacaa
    10921 acgtacaaaa catccaaatt ataatggaca cagaggaata tactatcctc aaagttacaa
    10981 ctaaaaataa acaggccata actatctgtt ttgcgaatac agataataat ccacattctg
    11041 tacataaaat aagagattat agctggcaag gagtctgcga aataaaataa
//
