LOCUS       CP002534               11822 bp    DNA     linear   BCT 16-JAN-2015
DEFINITION  Cellulophaga lytica DSM 7489, complete genome.
ACCESSION   CP002534
VERSION     CP002534.1
DBLINK      BioProject: PRJNA50743
            BioSample: SAMN00713590
KEYWORDS    GSC:MIGS:2.1.
SOURCE      Cellulophaga lytica DSM 7489
  ORGANISM  Cellulophaga lytica DSM 7489
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Cellulophaga.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Pati,A., Abt,B., Teshima,H., Nolan,M., Lapidus,A., Lucas,S.,
            Hammon,N., Deshpande,S., Cheng,J.F., Tapia,R., Han,C., Goodwin,L.,
            Pitluck,S., Liolios,K., Pagani,I., Mavromatis,K., Ovchinikova,G.,
            Chen,A., Palaniappan,K., Land,M., Hauser,L., Jeffries,C.D.,
            Detter,J.C., Brambilla,E.M., Kannan,K.P., Rohde,M., Spring,S.,
            Goker,M., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V.,
            Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Ivanova,N.
  TITLE     Complete genome sequence of Cellulophaga lytica type strain (LIM-21)
  JOURNAL   Stand Genomic Sci 4 (2), 221-232 (2011)
   PUBMED   21677859
REFERENCE   2  (bases 1 to 11822)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S.,
            Kyrpides,N., Mavromatis,K., Pagani,I., Ivanova,N., Ovchinnikova,G.,
            Teshima,H., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D.,
            Spring,S., Schroeder,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name             :: Cellulophaga lytica Cy l20, DSM 7489
            collection_date          :: 1969
            lat_lon                  :: 10.131928 -83.518524
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: Costa Rica
            environment              :: Mud
            num_replicons            :: 1
            ref_biomaterial          :: DSM 7489, ATCC 23178, IAM 14306
            biotic_relationship      :: Free living
            trophic_level            :: Chemoheterotroph
            rel_to_oxygen            :: Aerobe
            isol_growth_condt        :: 10425785
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            GOLD Stamp ID            :: Gi04010
            Type Strain              :: Yes
            Funding Program          :: DOE-GEBA 2009
            Gene Calling Method      :: Prodigal 1.4, GenePRIMP
            Isolation Site           :: Beach mud; Limon, Costa Rica
            Isolation Comments       :: isolated by R.A. Lewin
            Cell Shape               :: Rod-shaped
            Motility                 :: Motile
            Temperature Range        :: Mesophile
            pH                       :: 7.5
            Gram Staining            :: Gram-
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: Illumina, 454-Titanium
            ##Genome-Assembly-Data-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4087657
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Cellulophaga lytica DSM 7489"
                     /mol_type="genomic DNA"
                     /strain="DSM 7489"
                     /isolation_source="beach mud"
                     /culture_collection="DSM:7489"
                     /type_material="type strain of Cellulophaga lytica"
                     /db_xref="taxon:867900"
                     /country="Costa Rica: Limon"
                     /lat_lon="10.131928 N 83.518524 W"
                     /collection_date="1969"
                     /collected_by="R.A. Lewin"
     gene            complement(1..951)
                     /locus_tag="Celly_0292"
     CDS             complement(1..951)
                     /locus_tag="Celly_0292"
                     /note="COGs: COG0385 Na+-dependent transporter; InterPro
                     IPR002657; KEGG: lby:Lbys_2571 na+-dependent
                     transporter-like protein; PFAM: Bile acid:sodium symporter;
                     SPTR: Possible lipoprotein transmembrane protein; PFAM:
                     Sodium Bile acid symporter family"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P39836|2.A.28.3.4"
                     /protein_id="ADY28127.1"
                     /translation="MKIDKFVLFIIVAIVLAYFFPELGVKQVINTISSVGISLIFFFYG
                     LKLSPSKLKAGLKNWKLHILVQVATFILFPLLVLIVKPFLQNQEQETIWLAFFFLAALP
                     STVSSSVVMVSIAKGNMPAAIFNASISGIIGILVTPLWMGLFVTNDQTSFNFIDIYTKL
                     IVQIILPVVLGLLLQRYLGTFAQKHSSKITLFDKSIILLIIYKSFASSFTNNIFSTVSA
                     LDLVLLLAGVLVLFTITYFSTGFIATKLNFNIEDKITAQFCGTKKSLVHGTVFSKIIFG
                     NMAAIGIILLPIMLFHATQILIISVIASKLAKRKV"
     gene            1108..1872
                     /locus_tag="Celly_0293"
     CDS             1108..1872
                     /locus_tag="Celly_0293"
                     /EC_number="4.2.99.18"
                     /note="COGs: COG0708 Exonuclease III; InterPro IPR005135:
                     IPR000097: IPR004808; KEGG: cst:CLOST_2026
                     exodeoxyribonuclease III; PFAM:
                     Endonuclease/exonuclease/phosphatase; PRIAM: DNA-(apurinic
                     or apyrimidinic site) lyase; SPTR: Exodeoxyribonuclease III
                     Xth; TIGRFAM: Exodeoxyribonuclease III xth; AP
                     endonuclease, family 1; PFAM:
                     Endonuclease/Exonuclease/phosphatase family; TIGRFAM:
                     exodeoxyribonuclease III; exodeoxyribonuclease III (xth)"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III Xth"
                     /protein_id="ADY28128.1"
                     /translation="MHIVSWNVNGVRAVVKKEFFESVKYMNPDILCIQETKAQDNEVAK
                     ALSPLENFSLASNSANKKGYSGTATLSSTKPLTIKADIGIEAHDTEGRVLCTEYENFYL
                     VNVYVPNSGQKLDRLEYRKQWDKDFLDYLKNLEKKKPVIACGDFNVAHKAIDLKNDKSN
                     YNKTAGYTQTEIDGMNNFIADGFVDSFRMLHPDEVVYTFWSYRFKSRERNTGWRIDYFL
                     VSQSIKDKVKEVTVFSDIMGSDHCPIGLKIDF"
     gene            2306..4633
                     /locus_tag="Celly_0294"
     CDS             2306..4633
                     /locus_tag="Celly_0294"
                     /EC_number="4.2.2.3"
                     /note="InterPro IPR006626; KEGG: sde:Sde_3285
                     TonB-dependent receptor; PRIAM: Poly(beta-D-mannuronate)
                     lyase; SPTR: Putative alginate lyase"
                     /codon_start=1
                     /transl_table=11
                     /product="PL6_1|PL6"
                     /protein_id="ADY28129.1"
                     /translation="MQKHTLYLAFILLFSITSCTQKKNTVATLQQFNTAVKNAQPGDVI
                     TLANGVWNNTELLFAAKGTAEKPITLTVEEKGKVTLEGQSNLRIAGEHLIINGLVFKNG
                     YTPTNEVISFKQNAKTLANNTRLTECVIDNFSNPERHEPDTWVAIYGKNNRIDHNHLTG
                     KKNRGVTMTVRLNSKESQDNNHKIDHNYFGPRQNLGSNGGETLRIGTSHFSRTNSNTIV
                     ENNYFDRCNGEHEIISNKSCQNTYKNNVFYECTGTLTMRHGNRTMVDGNIFIGNNKPST
                     GGIRIINGEQTVINNYGIGLTGYRFRGAFVIMNGIYNSPINRYDQVKDAVVKNNTFVNS
                     NHIQLCAGSDEERSAPPVNSVMENNIFYNESKDSIFTVYDDINGITFTNNIISKNIKPI
                     TDTGFVSKDIEWVKNTDGLLVPANSNLRAGAKLPGEILKKEDTGVNWYSKKNTAVAFGT
                     GKKITVEPGLNTLFEAVNNSSAGDVVELTAGETYTLSKTIAINHPITILSNSDKKPTIL
                     FEKKTLFEINNGGSLKLTGINFDGESAPDRTGNSVITTSKYSMNKNYKLFIDNCDFVNL
                     DVNHSFDAVMVYKNTFADTISIKNSKFNTISGNVLALDKETEDIGIYNAEYVILKNNSF
                     NNVNGAVLSLHRGGKDESTFGPFLELDHNTFDAIGYGKRNKYNAAINLYGVQVNTITNN
                     IFNNTKSLKMHLVVGEPIVDILNNNFYKSDGLEVTGDQKYNVKNLWTLDPKFKEGTYTL
                     SKNSDLLNKGTDGKNLGLISNN"
     sig_peptide     2306..2371
                     /locus_tag="Celly_0294"
                     /note="Signal predicted by SignalP 3.0 HMM"
     gene            4637..6901
                     /locus_tag="Celly_0295"
     CDS             4637..6901
                     /locus_tag="Celly_0295"
                     /note="InterPro IPR012480; KEGG: cat:CA2559_11508 putative
                     chondroitin AC/alginate lyase; PFAM: Heparinase
                     II/III-like; SPTR: Heparinase II/III-like protein; PFAM:
                     Heparinase II/III-like protein"
                     /codon_start=1
                     /transl_table=11
                     /product="PL17_2|PL17"
                     /protein_id="ADY28130.1"
                     /translation="MIVKKYICFILICFLFACKKDNKAAIKNNVEQNTASHPSLILTQK
                     GVKNIKAQLGTIPLFDKTLEDVKQDVDAEISKGIAVPIPKDFSGGYTHEQHKKNFLILQ
                     KAGVLYQILDDEKYAVYVRDMFLEYAKLYPTLPLHPQERSYARGKLFWQCLNDSNWLVY
                     ASQAYDCIYNWLSKEEREHLENDLFIPFANFISEKNPQFFNRVHNHSTWGNVAVGMIAL
                     VMDNDELLQKALYGLKTDSIAANLKDDDGGFIKNKDQKVGFLANLDEPFSPDGYYTEGP
                     YYQRYAMYPFLIFAQALENKKPELKIFEHKNAVLTKAVEALINLTDKDGEFYTINDAQK
                     GMSYYSRELVTAVDIAYHYGGNNPELLSIAKKQNKVNLDDTGLAVALGLKNNLDKPFRK
                     KSITLTDGPNGNQGGISILRNNNLEVIFKYTAQGLSHGHYDKLSFFMYNNGTEVIQDYG
                     LARFVNIEQKGGGNYLKENTTWAKQTIAHNTVIQNETSHFNGKYSIGSKNHSNQYLFNI
                     SEKIKVVSAKENNAYPGTELHRTMAIVTDSSFIKQPFVLDIFNVIGTSTNKYDLPFYYK
                     GQIMQTNFKYTQQATLKTLGDKNGYQHLFVEALADAPNSNLKFNWMHNNVFYTLTSATQ
                     ATDKIIFARIGANDPEYNLRRDPTLIIRKTNTKNALYVNAIEAHGSYSPVSEFSVNAYS
                     SIAKIETLLNTKDYTAIRITNNNKQSKLFIFSNTNPDKTTAHSITINDNEFNWVGSYTY
                     N"
     gene            7250..10351
                     /locus_tag="Celly_0296"
     CDS             7250..10351
                     /locus_tag="Celly_0296"
                     /note="InterPro IPR012910; KEGG: gfo:GFO_1153
                     TonB-dependent outer membrane receptor; PFAM:
                     TonB-dependent receptor, plug; SPTR: Putative outer
                     membrane protein, probably involved in nutrient binding;
                     PFAM: TonB-dependent Receptor Plug Domain; TonB dependent
                     receptor"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="ADY28131.1"
                     /translation="MNLKTKLILVMTLFCSAITIAQESFPIKGTVVSSADNLPILGVNI
                     IIKNTATGTTTDFDGNYEIKAKKGDVLQFSFIGFITKSITVADQTTINVTLDEDASKLD
                     EVVVIGYGTQKKSHLTGSISKVVNEDLGQIAVSRVDDALVGQVSGVNIQATEGEAGSDP
                     TIRIRGTGSITGNSGPLVVVDGVVVPNDYLGSLDMNDVESFEILKDAASGAIYGSRGGN
                     GIIQITTKSGKSGETRFSYSTLTGVKTARQSDAYYSTVAETAAAELQFTHTLSDRTRYK
                     QLIGVDTNWQEIIFDGGIITNHSLGIRGGNDKLRFSTNLNYNHDEGVLLTDDFKKYSVK
                     VRLDYDVTDNLTIGLSATPSYTERRRFDGSTHDILRQPSWLPVYHDENTIQFVNRVQDG
                     GKWADVQVGDYALQYHFDGYDLDAGAPADSGGTSISNTSNTNPAAKVLERDRRDFRSKI
                     LGSFYAKYKINDDLSFKTTLSGDYQRYRQQRWQGVEANRNGASAAQLDSTNTRTQHLAL
                     DNVLSYNKTFDKHDLGIVLGASGEQYKTEFETISSQGYTDDSSRYINRDDITNEYEEEY
                     ESTLLSFFTRVNYAYSDKYLASFSFRRDGASVFGPNNKYGNFFAGSLGWNISRENFLKN
                     SDVVNNLKFRVSYGVTGNNSFRTGNNLINNYPYLSLIDNSIASGVTGGVVTNAANPLNI
                     SNPDLKWERQVEFNPGVDFGFFGNILSGSVDYYKRTSDQLLLNNPIASTTGFTNALVNI
                     GEVENSGVEVELRTRNVAGPKFKWTTTFITSTNKNELTDFADSNGQIQNVDSKRASEWI
                     NLEGNPISSFYGWVVERDIPLEFLNNPFHPVGGEAQDVYVKDLNGDGIIDDDDKTILGN
                     PYPEFVWSVSNDFKIGNFDFSFMFQGSHGAEIRNMGDQYIFNHFNSSQDFVTGPTGDYV
                     LTTPNQEFIKQKIFTNDIIQDASYIALRNINLGYNFSKDVASQLKIRSARIYVSAQNIM
                     YLTADDYTGFNPESINTTSATTYGYQRAGSPVFSTISAGLNIEF"
     sig_peptide     7250..7312
                     /locus_tag="Celly_0296"
                     /note="Signal predicted by SignalP 3.0 HMM"
     gene            10377..11822
                     /locus_tag="Celly_0297"
     CDS             10377..11822
                     /locus_tag="Celly_0297"
                     /note="InterPro IPR012944; KEGG: cat:CA2559_11493 putative
                     outer membrane protein; PFAM: RagB/SusD; SPTR: Putative
                     outer membrane protein, probably involved in nutrient
                     binding; PFAM: SusD family"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|C6Y217|8.A.46.1.3"
                     /protein_id="ADY28132.1"
                     /translation="MKKLKFLTLIVITIFSVSCEDSFLSPELKTGTSGDAFYSNDEELE
                     AAIINIYDGLQGVNASKLTNSNLNHATQVEFYVTEMRSDNTRTKSQEGEAAQFESFAIE
                     ATNGIVADYYRSFYDVIFRANVVLDNIGSASEATAGKIEGEAKFLRAYAYFNLVRLYGD
                     VPLVTNVIAPLDTETAFTRVNSAEIYNVIVADLQTAVDNLDDTYKDRASKAAAQGLLAK
                     VYLSLPTPNYAQAQLLCEEVMNPARGYSLLTNYSDIFFSELNNEVIFAIGYEQGDNNNS
                     QNFSAEWLNSVGRTSGVNYATTDVVAALEEMGGDRTQYTYRVDPFQPTENQAIKYLPNG
                     ESGGDNGRTFSSDAQAAGNDWIVLRYADVVLLHVEAILGGGESTSNSNALTSFNSIRNR
                     AGLPDDEDGVITKEELLNERRVELAFENKRLFDLIRMGEAVNVLSEFATENGYSFSSTD
                     LLLPIPQREIGLSKGVLTQNPGY"
     sig_peptide     10377..10436
                     /locus_tag="Celly_0297"
                     /note="Signal predicted by SignalP 3.0 HMM"
ORIGIN
        1 ttatactttt ctttttgcta gtttgcttgc aattacactt attattaaaa tttgtgttgc
       61 gtgaaaaagc attataggca gtaatataat gcctattgct gccatattac caaatattat
      121 tttagaaaaa acagtaccat gtactaatga tttttttgtg ccgcaaaact gagccgtaat
      181 tttatcttct atattaaaat taagctttgt agctataaag cctgtactaa agtaagtaat
      241 ggtaaaaaga accaatacac ctgctaaaag tagtactaaa tctaatgcag agactgtact
      301 aaaaatatta tttgtaaagg atgacgcaaa acttttgtaa attattaata atataataga
      361 cttgtcaaac aatgtaattt tactactgtg tttttgtgca aatgtaccaa ggtaacgttg
      421 caacaataaa cctaatacta caggcaatat tatttgtaca attagctttg tataaatatc
      481 tataaaatta aaacttgttt ggtcattagt aacaaataac cccatccata agggtgtaac
      541 taaaatccct ataatgccag atatacttgc attaaaaata gctgcaggca tattaccttt
      601 tgctatagaa accattacaa cagatgaaga tactgtagat ggtaatgctg ctaaaaaaaa
      661 gaatgcaagc caaatagttt cttgctcttg gttttgtaaa aatggtttaa ctattaatac
      721 caaaagagga aacaatataa aggtagctac ttgcactaaa atatgcagtt tccaattctt
      781 taaaccagct tttaatttag acggacttaa ttttaatccg taaaaaaaga aaattaaaga
      841 tataccaaca ctactaattg tatttataac ctgttttact cctagttctg gaaaaaaata
      901 ggccaaaaca attgctacaa ttataaacag tacaaactta tcaatcttca taatctactt
      961 ttctaaaata caatattaag cacttgttac ttcacattaa aaataatctc tcttaaaata
     1021 cttttatttt tgaggttaaa ccaattcaat tttttaattt catcctagaa atacaatatt
     1081 ttattttaac ccataaagta acgactaatg cacatagtat cttggaatgt aaatggtgta
     1141 cgcgctgtag taaaaaaaga gttttttgaa tctgtaaaat atatgaatcc agatatactt
     1201 tgtattcaag aaactaaagc acaagataat gaggttgcga aggcactttc tccattagaa
     1261 aactttagtt tagcatctaa ctctgccaat aaaaaagggt attctggtac tgcaacatta
     1321 agtagcacaa aaccccttac tattaaagca gatattggta tagaagcaca cgatactgaa
     1381 ggtagagtac tttgtactga gtatgaaaat ttttacttgg taaatgttta cgtacctaat
     1441 tctggtcaaa aattagatag gttagagtac agaaaacagt gggataaaga ctttttagac
     1501 tatttaaaaa atttagaaaa gaaaaagcct gtaattgctt gtggcgattt taacgtagca
     1561 cacaaagcaa tagacctaaa aaatgataaa agtaactaca ataaaactgc tggttataca
     1621 caaacagaaa ttgatggtat gaacaatttt atagctgatg gttttgtaga ttcttttaga
     1681 atgctacacc cagatgaagt tgtttatacg ttttggagct accgttttaa atctagagaa
     1741 cgcaataccg gttggcgtat agattatttt ttagtaagcc aatctataaa agacaaagtt
     1801 aaagaggtaa ctgttttctc tgacattatg ggatctgacc actgccctat tggacttaaa
     1861 attgattttt aatttaaggt attaagtata tagaataaaa atttctatag gcgtttttta
     1921 gtgttattaa ttatcataat ttattaaata tttataaatg caaatttata ttttagtcac
     1981 taccttaaat tgtaacattg tatttatata aggtcaatta taaaagttat aaaacaatat
     2041 tttataactt aaaactgata ttctgttata gagttaaaat tcttcatttt taggtattct
     2101 ataaattact agcgtacaat cctctagcac gccagtatta acaatgcaat aataaaaaaa
     2161 taacacctta ataacccatt agcaactacg tattttttta tttatttttt tacactatta
     2221 gtaatttaac agtcaataaa ttacattttt aatttataat atatatattt ggtaaaccaa
     2281 tctggttaac caattttata tttttatgca aaaacataca ttatacttag cttttatact
     2341 actttttagt attacttcat gcacacaaaa aaagaatacc gtagctactt tacaacaatt
     2401 taacactgca gtaaaaaatg cacaacctgg agatgtaatt acactggcta atggcgtatg
     2461 gaacaatacc gaattacttt ttgctgcaaa aggcacagca gaaaagccaa taactttaac
     2521 tgtagaagaa aaaggtaagg taactttaga agggcaaagt aacttacgta ttgcaggtga
     2581 acacttaatt attaatggtt tggtatttaa aaatggttat actcccacta atgaagttat
     2641 ctcttttaaa caaaatgcta aaactcttgc aaataacact agactaacag aatgtgttat
     2701 agataatttt agtaacccag aacgtcacga accagatact tgggttgcta tatacggtaa
     2761 aaataaccga attgaccaca accatttaac tggtaaaaaa aatagaggtg ttactatgac
     2821 ggttaggtta aattctaaag aaagtcaaga caacaatcat aaaatagatc acaactattt
     2881 tggacctaga caaaacttag gctctaacgg tggtgagact ttacgtattg gaactagcca
     2941 tttttctaga acaaactcta atactattgt agaaaataat tattttgata ggtgtaacgg
     3001 agaacatgaa ataatctcta acaaatcatg tcaaaacaca tacaaaaaca atgtttttta
     3061 tgagtgtaca ggtacattaa ctatgcgcca cggtaacaga actatggtag atggcaacat
     3121 atttattggc aacaataaac ctagcacagg tggtattagg attattaatg gtgagcaaac
     3181 tgttataaat aattatggta ttggattaac tggttaccgt tttagaggtg cttttgttat
     3241 tatgaacgga atttacaatt ctcctattaa taggtatgac caagttaaag atgctgttgt
     3301 aaaaaataac acttttgtta atagtaacca cattcagtta tgtgcaggta gtgatgaaga
     3361 acgtagtgct cctcctgtaa actctgttat ggaaaataat attttttata atgagagtaa
     3421 agattcaatt tttacggttt atgatgatat taatggtatt acatttacaa ataacattat
     3481 tagtaaaaac ataaaaccaa ttactgatac tggatttgta agtaaagata ttgagtgggt
     3541 aaaaaataca gacggattgc tagttcctgc taactctaac ttaagagctg gtgctaaatt
     3601 accaggtgaa attcttaaaa aagaagatac aggtgttaac tggtactcta aaaaaaatac
     3661 tgctgttgcc tttggtactg gtaaaaaaat tacagtagaa ccaggtttaa atactttgtt
     3721 tgaagctgta aataattctt cagccggaga cgtagtagag cttactgctg gcgaaactta
     3781 tactttatca aaaactatag ctataaatca tcctattact atcctttcta attcagataa
     3841 aaaacctact attttatttg aaaagaaaac cttatttgaa attaacaatg gaggcagctt
     3901 aaaattaact ggaattaatt ttgatggtga aagcgcccca gacagaacag gaaattctgt
     3961 aattactact agtaaatact caatgaacaa aaattacaaa ttgtttatag ataattgtga
     4021 ctttgtaaac ttagatgtta accattcttt tgatgctgta atggtgtaca aaaacacatt
     4081 tgcagacaca attagtatta aaaattcaaa atttaatact attagtggta acgttttggc
     4141 tttagataaa gaaactgaag atattggtat ctataatgca gaatatgtaa tcttaaaaaa
     4201 taatagtttt aacaatgtaa atggtgctgt tttaagttta cacagaggtg gtaaggatga
     4261 aagtactttt ggtccgtttt tagaattaga ccataacact tttgacgcta ttggttacgg
     4321 taaaagaaat aaatacaatg ctgcaattaa cttatatggt gtgcaagtaa atactattac
     4381 caacaatata tttaacaaca caaaatcgtt aaaaatgcat ttagttgttg gtgagccaat
     4441 agtagatatt ttaaacaata atttttacaa atctgatggt ttagaagtta ctggtgatca
     4501 aaaatacaat gttaaaaacc tatggacatt agatcctaaa tttaaagaag gtacttatac
     4561 attatctaaa aattctgacc ttttaaataa aggtacagat ggcaaaaatt taggattaat
     4621 tagtaataac tagataatga ttgtaaaaaa atatatatgc tttattttaa tatgttttct
     4681 ttttgcttgc aaaaaagata ataaagctgc cattaaaaat aatgtagaac aaaatactgc
     4741 aagccaccct agtttaatac ttacccaaaa aggcgtaaaa aatattaaag cacagttagg
     4801 aacaattcct ttgtttgata aaactttaga agatgttaaa caagatgtag atgcagagat
     4861 tagtaaaggt attgctgttc ctattcctaa agatttttct ggaggttata cgcatgaaca
     4921 gcacaaaaaa aactttctaa tactacaaaa agctggtgtg ctttatcaaa ttttagatga
     4981 tgaaaaatat gctgtatatg taagagatat gtttttagag tacgcaaaat tataccctac
     5041 tcttccttta cacccacaag aaagatctta tgcaagagga aaactttttt ggcagtgttt
     5101 aaatgattct aactggttgg tatatgctag ccaagcatac gattgtatct ataattggtt
     5161 atcaaaagaa gaaagagaac atttagaaaa tgatttattt attccgtttg ccaattttat
     5221 atcagaaaaa aatccacagt tttttaatag agtacacaac catagtacct ggggtaatgt
     5281 tgctgtagga atgattgctc ttgtaatgga taatgatgag ctattacaaa aagctctata
     5341 cggactaaaa acagattcta ttgcggctaa tttaaaagat gatgatggtg gatttataaa
     5401 aaataaagat cagaaagttg gttttttagc aaacttagat gaaccatttt ctcctgacgg
     5461 atattatact gaaggtcctt attaccaaag gtatgctatg tacccatttt taatttttgc
     5521 acaagcacta gaaaataaaa aacctgaact taaaattttt gagcataaaa atgcagtact
     5581 aacaaaagct gtagaagcat taattaacct aactgataaa gacggagagt tttacacaat
     5641 taatgatgca caaaaaggaa tgtcttacta ctccagagaa ttagtaacag ctgtagatat
     5701 tgcttaccat tacggaggca ataacccaga actattaagt attgcaaaaa aacaaaataa
     5761 agtaaattta gacgatacag gtttagcagt agcattgggt ttaaaaaaca atctagataa
     5821 accttttaga aaaaaatcta ttactttaac agatggtcca aatggcaacc aaggtggcat
     5881 aagtatatta cgcaataata atttagaggt tatttttaaa tatacagcac aaggtcttag
     5941 tcacggtcat tatgacaagt tatctttttt tatgtacaat aatggtacag aggttataca
     6001 agattatggc ttggcaaggt ttgttaatat agaacaaaaa ggtggcggta actacttaaa
     6061 agaaaatact acttgggcaa aacaaacaat agcgcataat accgttatac aaaatgaaac
     6121 ttctcatttt aatggcaagt atagtatagg tagcaaaaac cactctaacc aatacttatt
     6181 taatatttct gaaaaaatta aagtggttag cgctaaagaa aataacgcat accctggcac
     6241 agagttacac aggactatgg ctatagtgac agactcttct tttattaaac aaccatttgt
     6301 tttagatatt tttaatgtta ttggtacatc aacaaacaaa tacgatttgc ctttctatta
     6361 caaggggcaa ataatgcaaa caaattttaa gtatacacaa caagctacat taaaaacatt
     6421 aggagacaaa aatggctatc aacatttatt tgtagaggct ttagcagacg caccaaatag
     6481 taacttaaaa tttaattgga tgcacaataa tgtattttac acattaactt ctgctacaca
     6541 agcaacagac aaaattattt ttgccagaat tggtgctaac gatccagaat ataatttaag
     6601 aagagatcct actttaatca ttagaaaaac taatactaaa aacgcattat acgtaaatgc
     6661 catagaagca catggtagct acagtcctgt atcagagttc tctgtaaatg cttacagtag
     6721 tattgctaaa atagaaaccc ttttaaatac taaagactat acagctatac gcattacaaa
     6781 caataataaa cagtctaaac tatttatttt ttctaataca aacccagaca aaactacagc
     6841 acattcaatt acaattaatg ataatgaatt taactgggta ggttcataca cttataacta
     6901 acttaatttt atctatatga aaaaaaatga aatgtctttt atcctaatac agagagtttt
     6961 acaaaaaaac ttattaatgt gtatttaatt tttttttaac aaaattttta catatattcg
     7021 aaaagtataa actggttaac cagtttggtt aaccaaattt aaatagagca caaaaaaagg
     7081 ataggtgcac gcctatcctt ttacaaatac ttctttggag ggaagtaata aatatgtagt
     7141 ataaaaacat attctttaca aaagtaatga ttatttgctt ttagcctaga gaaatggttt
     7201 ttaagttact acagaacact attaactcaa ttaaactaaa caattaatta tgaacttaaa
     7261 aacaaagcta attttggtta tgacactatt ttgtagtgct ataactatag ctcaagaaag
     7321 ctttcctata aaaggtacgg tagtctcttc tgcagacaac ttacctattt taggagtaaa
     7381 cattatcatt aaaaatactg ctacaggcac aactacagat tttgatggca actatgaaat
     7441 taaagctaaa aaaggtgatg ttcttcaatt ttcttttatt ggatttatta ctaaaagtat
     7501 tactgtagca gaccaaacta ccattaatgt tactttagat gaagatgcta gtaagttaga
     7561 tgaagttgtg gttattggtt atggtacaca aaaaaaatct cacctaacag gttctatttc
     7621 taaagtagta aacgaagatt taggacaaat tgctgtttct agggtagatg atgccttagt
     7681 aggtcaagta tctggtgtta acatacaagc tacagaaggt gaagctggtt ctgatcctac
     7741 aattagaatt agaggaacag gttctattac aggtaactct ggaccactag ttgttgtaga
     7801 tggtgttgtg gttcctaatg actatttggg ttctttagat atgaatgacg ttgaatcttt
     7861 tgaaatttta aaagatgcag cttctggtgc tatttatggt tctagaggtg gtaatggtat
     7921 tatacaaatt actacaaaat ctggtaaatc gggtgaaact aggtttagct actctacatt
     7981 aactggtgtt aaaacagcaa gacagagtga tgcttactac tctacagtag cagaaactgc
     8041 cgctgcagaa ttacagttta cacatacact ttcagaccgt acaagataca aacagttaat
     8101 tggcgtagat accaactggc aagaaattat ttttgatggt ggtattatta caaaccactc
     8161 tttaggtatt agaggtggta atgataaatt aagatttagt acaaacctaa actacaacca
     8221 tgatgaaggg gttttactta ctgatgattt taaaaaatac agtgtaaaag ttagattaga
     8281 ttatgatgtt acagataact taacaattgg tcttagtgct actccttctt acacagaacg
     8341 tagacgtttt gatggtagta cacatgatat tttaagacaa ccttcatggc taccagttta
     8401 ccatgatgaa aacactatac aatttgtaaa cagagtgcaa gatggtggta aatgggcaga
     8461 cgtacaagtt ggcgattatg ctttacaata ccattttgat gggtatgatt tagatgctgg
     8521 tgctcctgca gacagtggag gtacaagtat tagtaacact tctaacacta accctgctgc
     8581 aaaagtacta gaaagagacc gtagagattt tagaagtaaa attcttggta gcttttatgc
     8641 taaatacaaa attaatgacg atttaagctt taaaactaca ctttctggtg attaccaaag
     8701 atacagacaa caaagatggc aaggtgtaga ggctaacaga aacggagcaa gtgctgctca
     8761 gttagatagt acaaatacca gaacacaaca cttagcttta gataatgtat tatcatacaa
     8821 caaaactttt gataagcatg atcttggcat agtattaggt gcatctggtg aacaatataa
     8881 aacagaattt gaaactatta gctcacaagg ttatacagat gatagttctc gttacattaa
     8941 cagagatgac attactaatg aatatgaaga agagtacgaa agtacattac tatcattctt
     9001 tacaagagta aattatgcgt attcagacaa atacttagct tcttttagtt ttagaagaga
     9061 tggtgcttct gtatttggtc caaacaataa atatggtaac ttctttgcag gttctttagg
     9121 ttggaacatc tctagagaaa acttcttaaa aaatagtgat gtagttaata accttaaatt
     9181 tagagttagt tatggtgtaa ctggtaacaa ctcttttaga accggtaata acttaattaa
     9241 caactaccct tacctatctt taatagacaa ttctattgcc tctggtgtta caggtggtgt
     9301 ggttacaaat gctgctaacc ctttaaatat atctaatcca gatttaaaat gggaaagaca
     9361 agtagagttt aaccctgggg tagactttgg tttctttggt aatattttat ctggttctgt
     9421 agattactat aaaagaacta gtgaccaact actactaaat aacccaattg cctctacaac
     9481 tggttttact aatgctcttg taaatattgg agaggtagaa aactctggtg tagaagttga
     9541 actaagaact agaaacgttg ctggtcctaa atttaaatgg acaacaactt ttattacatc
     9601 taccaataaa aatgaattaa cagattttgc tgactctaac ggacaaatac aaaatgtaga
     9661 ttctaaaaga gcttcagagt ggataaattt agaaggtaac cctatctcat ctttttacgg
     9721 gtgggttgta gaaagagaca ttcctttaga atttttaaac aatccttttc acccagtagg
     9781 cggtgaagca caagatgttt atgtgaaaga tttaaatggt gatggcatta ttgatgatga
     9841 tgataaaact atattaggaa atccttaccc agaatttgtg tggagtgtat ctaacgactt
     9901 taaaataggc aattttgatt ttagttttat gtttcaaggt agccacggag cagaaattag
     9961 aaatatggga gaccaataca tatttaacca ctttaacagt tctcaagatt ttgtaactgg
    10021 tcctacagga gattatgtat taactactcc taatcaagaa tttattaaac aaaaaatatt
    10081 tactaatgac attattcaag atgcttcata catagcatta cgtaatatta accttgggta
    10141 taactttagt aaagatgttg catctcaatt aaaaattaga agcgcaagaa tttatgtatc
    10201 tgcacaaaac ataatgtacc taacagcaga tgactatact ggttttaacc cagagtctat
    10261 taatactaca tctgcaacca cttatggtta ccaaagagct ggttctccag tattcagtac
    10321 aatctcagca ggtttaaaca tagaatttta aactactaat ttaaaaatat ataatcatga
    10381 aaaaacttaa atttttaaca ttaatagtca ttacaatttt ctctgtatct tgtgaagatt
    10441 catttttatc accagaacta aaaactggta caagcggaga tgctttttat tctaacgatg
    10501 aagaattaga agctgcaata attaatattt acgacggttt gcaaggtgta aatgcatcca
    10561 agcttacaaa ctctaactta aaccacgcaa cacaagtaga gttttacgtt acagaaatgc
    10621 gtagtgataa tacaagaaca aaaagtcaag aaggagaagc tgcacagttt gaaagttttg
    10681 ctatagaagc tactaacggt attgtagctg attattacag aagtttttac gatgtaattt
    10741 ttagagcaaa tgttgtttta gataacattg gttctgcatc agaagctact gctggtaaaa
    10801 ttgaaggtga ggctaagttt ttaagagctt acgcatactt taatttggtt cgtttatatg
    10861 gcgatgttcc tttagttact aacgttattg caccattaga tacagaaaca gcttttacaa
    10921 gagttaactc tgcagaaatt tacaatgtaa ttgttgcaga cttacaaact gctgtagata
    10981 atttagatga tacttataaa gacagagctt ctaaagctgc tgcacaaggc ttactagcca
    11041 aagtatattt atctctacca actcctaact atgcacaagc gcaattgctt tgcgaggaag
    11101 ttatgaaccc tgcaagaggt tatagtttat taaccaatta cagtgatatc ttcttctctg
    11161 aacttaataa cgaagttatt tttgctattg gttatgaaca aggagacaat aataacagtc
    11221 aaaatttttc tgcagaatgg ttaaactctg ttggcagaac tagtggcgta aactacgcta
    11281 ctacagatgt tgttgctgcg ttagaagaaa tgggcggaga cagaactcaa tatacataca
    11341 gagtagaccc ttttcagcct acagaaaacc aagcaattaa atatttgcca aacggagaaa
    11401 gcggtggaga caacggaaga acttttagtt ctgatgcaca agctgctggt aatgattgga
    11461 ttgtcttaag atacgcagat gttgttttac ttcatgtaga agccatttta ggcggtggtg
    11521 aatctacctc taactcaaat gcacttacat catttaactc tattagaaat agagctggct
    11581 tacctgatga tgaagacggt gttattacaa aagaagaatt gctaaatgaa agacgtgtag
    11641 aacttgcgtt tgagaacaaa cgtttgtttg atttaattag aatgggagaa gctgtaaatg
    11701 ttttatcaga atttgctaca gaaaatggat atagtttttc ttctacagac ttgttattac
    11761 ctattccaca aagagaaata ggcttaagta aaggtgtatt aacacaaaac cctggttact
    11821 aa
//
