LOCUS       KOU61727                3850 bp    DNA     linear   BCT 22-JUL-2016
DEFINITION  Klebsiella oxytoca PTS antiterminator (casR) gene, partial cds,
            cellobiose-specific PTS permease (casA) and phospho-cellobiase
            (casB) genes, complete cds.
ACCESSION   U61727
VERSION     U61727.1
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Lai,X., Davis,F.C., Hespell,R.B. and Ingram,L.O.
  TITLE     Cloning of cellobiose phosphoenolpyruvate-dependent
            phosphotransferase genes: functional expression in recombinant
            Escherichia coli and identification of a putative binding region for
            disaccharides
  JOURNAL   Appl. Environ. Microbiol. 63 (2), 355-363 (1997)
   PUBMED   9023916
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Lai,X., Davis,F.C., Hespell,R.B. and Ingram,L.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-1996) Microbiology and Cell Science, University of
            Florida, Gainesville, FL 32611, USA
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="P2"
                     /db_xref="taxon:571"
     gene            <1..291
                     /gene="casR"
     CDS             <1..291
                     /gene="casR"
                     /note="regulatory protein"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS antiterminator"
                     /protein_id="AAB51562.1"
                     /translation="LQLVKYQLTLNYDEESLSYQRFVTHLKFFAQRMLTRTVVEDDDVT
                     LHSAVKDNYPKAWKCAETVARHLQKSYQRPLTTEEIMFLAIHIERVRKEGR"
     gene            456..2321
                     /gene="casA"
     CDS             456..2321
                     /gene="casA"
                     /note="PTS enzyme II homolog"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P08722|4.A.1.2.2"
                     /protein_id="AAB51563.1"
                     /translation="MEYKALAQDILTRVGGRENIVSLVHCATRLRFKLKDSKKADAEGL
                     KANPGVIMVVESGGQFQVVIGNHVHDVWLAVRSEAGLTDDSEPVAVKGEKVSLIGQLID
                     IVSGIFTPFIGVLAASGILKGLLALAVVCGWLTTQQATYKIWFAASDALFFFFPLFLGY
                     TAGKKFGGNPYITMVIGGALTHPLMIQAFDASMAPGAATEYFLGIPVTFINYSSSVIPI
                     ILASWVSCWIEKRSNAILPSSMKNFFTPAICLAVVVPLTFLIIGPVATWLSQLLANGYQ
                     LIYQVAPWLAGAAMGALWQVCVIFGLHWGLIPLMINNLAVLGHDSMMPMLLPAVMGQVG
                     AALGIFLRTRDARQKVLAGSAVSAGIFGVTEPAIYGLNLPLRRPFIFGCVYWPIGGAMV
                     GFSDSHVSTYSFGFGNIITLAQMIPPEGIDATVWGGAAGMFASLIIACVLTLVAGLPRS
                     SAEQAAVVVAPASVNDILAPMTGSVLALDQVPDSTFASGLLGQGVAIIPSVGKVIAPFS
                     GEVASIFQTKHAIGLLSDSGIELLIHVGIDTVKLDGAPFTAHVKEGDKIKAGDLLLEFD
                     RQAILDAGYDLATPIIISNSDDFRTLDMVSASAVDAGQPLLSVSR"
     gene            2339..3733
                     /gene="casB"
     CDS             2339..3733
                     /gene="casB"
                     /note="glycohydrolase homolog"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1"
                     /protein_id="AAB51564.1"
                     /translation="MKTFPQAFLWGGATAANQVEGAYLEDGKGLTTSDVQPRGVFGDVV
                     ERVPGDSGIKDIAIDFYHRYPEDISLFAEMGFNCLRVSIAWARIFPHGDEAQPNEAGLA
                     FYDKLFDEMAKHNITPLVTLSHYEMPWALVKNYGGWGNRKVIGFFERYARTVFERYQAK
                     VKLWLTFNEINMSLHAPMTGVGLPADSSKAEVYQAIHHQLVASALAAKACHDIVPEGKI
                     GNMLLGGLMYPLSCKPDDIFETLQQNRSWQFFGDVQCRGAYPGYMLRYFRDNGINLDIT
                     DADRAALKETVDFISFSYYMTGCVTADEELNKKARGNILSMVPNPHLASSEWGWQIDPL
                     GLRTLLNVLWDRYQKPLFIVENGLGAKDKVEADGSINDDYRISYLNDHLVQVREAIEDG
                     VELMGYTSWGPIDLVSASKAEMSKRYGFIYVDRDDDGNGTLARSRKKSFWWYKEVIATN
                     GGSLKE"
ORIGIN
        1 ctgcaactgg tgaagtatca gctgacgctg aactacgacg aagagtcgct gagctaccag
       61 cgctttgtca cccacctcaa gtttttcgcc cagcgcatgc tgacgcgaac ggtggtcgaa
      121 gatgatgatg tgacgctgca cagcgcggta aaggataact accctaaagc gtggaagtgt
      181 gcggaaaccg tcgcccggca cctgcaaaag agctaccagc ggccactgac gacggaagag
      241 attatgtttc tcgccattca tattgagcgg gtgagaaaag agggacgcta agcgccgata
      301 gggcctggcg catagctccc gcgctacaac tggattgtta ctgcattacg caggcaaaac
      361 ctgagccgac gcctacacgg cgttgttctc gggttttttt atttttaata ctcagttaac
      421 gatgcctgcg ggcagcggaa gtaaggaaaa acagcatgga atataaagca ctcgcgcagg
      481 atattctcac tcgggtaggc ggcagagaga atattgtgag tctggttcac tgcgctacac
      541 ggctgcgttt taagctcaaa gatagcaaga aggcggatgc cgaaggactg aaggcgaacc
      601 ctggcgtcat tatggtcgtc gaaagcggcg gccagtttca ggtggttatc ggtaatcacg
      661 tgcacgatgt ctggctggcg gtgcgcagcg aagccggcct gacggatgac agtgagccgg
      721 tggcggtaaa aggcgaaaaa gtgtcgctta tcggccagct tatcgacatt gtttccggta
      781 ttttcacccc gtttatcggc gtcctggcgg cctcggggat cctcaaaggc ttgctggcgc
      841 tggcggtggt ttgcggctgg ttaacgacgc agcaggcgac ctataaaatc tggtttgccg
      901 ccagcgatgc gctgttcttc tttttcccgc tgtttctcgg ctataccgca ggcaagaaat
      961 ttggcggcaa cccctatatt accatggtga tcggcggcgc gttaactcat ccgctgatga
     1021 ttcaagcgtt tgacgccagc atggctcccg gcgccgcgac ggagtatttc ctcggtattc
     1081 cggtgacctt tatcaattac agctcctcgg tcattccgat tatcctcgcc tcgtgggtca
     1141 gctgctggat tgaaaaacgc agcaacgcga tactgccttc ctcgatgaag aacttcttca
     1201 cgccggcgat ttgcctggcc gtcgtcgtac cgctcacgtt tctgattatc ggccccgtag
     1261 ccacctggct gagccagctg ctggccaatg gttatcagct tatctatcag gttgcgccct
     1321 ggcttgccgg agcggcgatg ggagcgctgt ggcaggtatg cgttatcttc gggctgcact
     1381 ggggcctgat accgttaatg attaacaacc tcgccgtgct gggccatgat tcgatgatgc
     1441 cgatgctgct gcctgcggtg atgggccagg tgggcgccgc gctggggatt ttcctgcgca
     1501 ctcgcgatgc gcggcaaaaa gtgctggcgg gttctgcggt ttccgccggg atctttggcg
     1561 tgaccgagcc ggcgatttat ggtctgaacc tgcctttacg ccgtcccttt atcttcggct
     1621 gcgtttactg gcccatcggc ggcgcgatgg ttggttttag cgacagccac gtctcgacct
     1681 actcttttgg tttcggtaac attatcaccc tggcgcaaat gatcccgccg gagggcattg
     1741 acgctacggt gtggggcggc gcggcgggga tgtttgcctc gttaatcatc gcctgcgtgc
     1801 ttaccctcgt cgccgggctg ccgcgcagca gcgctgagca ggctgcggtt gtcgtggcgc
     1861 cggcctcggt aaacgacatc ctggcgccga tgaccggcag cgtgctggcg ctggatcagg
     1921 tgcctgacag taccttcgcc agcggcctgt tgggccaggg cgtggcgatt attccctcag
     1981 tgggcaaggt tatcgcgccg ttctcgggcg aagtcgcctc catttttcaa accaaacacg
     2041 ccatcggtct gctgagcgac agcggcattg agctgctcat ccacgttggc atcgacaccg
     2101 ttaagctcga tggcgcgccg ttcaccgccc atgtaaaaga gggtgacaaa atcaaagcgg
     2161 gcgacctgct gctggagttt gaccgccagg cgattctgga cgccggctat gatctggcga
     2221 cgcccattat tatcagcaac agcgacgatt ttcgtactct cgacatggtc tccgccagcg
     2281 cggtggatgc cggtcagccg ctgttgtccg taagccgtta atcacaggag agagatgaat
     2341 gaagacattc ccacaggcgt ttttatgggg cggcgcgacc gcggcaaacc aggttgaagg
     2401 cgcttatctg gaagacggga agggattaac cacttctgac gtccagccgc gcggcgtatt
     2461 tggcgatgtg gtagaacgcg tgcccggcga tagcggcatt aaggatatcg ctatcgactt
     2521 ttaccaccgt tatccggaag atatcagcct ctttgccgag atgggcttta actgcctgcg
     2581 cgtatcgatt gcctgggcgc gtattttccc ccacggcgat gaggcgcagc caaacgaagc
     2641 cgggctggcc ttctacgata agctgtttga tgaaatggcg aagcacaata ttactccgct
     2701 ggtaaccctg tcccactatg agatgccgtg ggcgctggtg aaaaactacg gcggctgggg
     2761 caatcgcaaa gtgattggct tcttcgagcg ctacgcccgt acggtgttcg agcgctacca
     2821 ggcgaaggtc aaactgtggc tgaccttcaa tgaaatcaat atgtccctgc acgcgccgat
     2881 gaccggcgtc ggtctgccgg ctgacagcag caaagcggaa gtctaccagg ctattcacca
     2941 tcagctggtg gcgagcgcgc tggcggctaa ggcctgccat gacatcgtgc ctgagggcaa
     3001 aatcggcaat atgctgctcg gcggcctgat gtatccgctc agctgcaaac cggacgatat
     3061 ttttgaaacc ctgcagcaga accgtagctg gcagttcttt ggcgatgtcc agtgccgcgg
     3121 cgcttacccg ggctatatgc tgcgctattt ccgcgacaac ggcattaacc tcgacatcac
     3181 cgatgccgac cgcgccgccc tgaaagagac cgtcgacttt atctccttca gctactatat
     3241 gaccggctgc gtgaccgccg acgaagagct gaacaaaaaa gcgcgcggca atattctcag
     3301 catggtgccg aacccgcacc tggcaagctc ggaatggggc tggcagattg acccgctcgg
     3361 tctgcgcacg ctgctgaacg tgctgtggga tcgctatcag aagccgctgt ttatcgtgga
     3421 aaacggcctg ggcgccaagg ataaggtgga agccgacggc agcatcaacg acgattaccg
     3481 gatcagctat cttaacgacc atctggtgca ggttcgtgaa gcaatcgagg acggcgttga
     3541 gctgatgggc tacaccagct gggggcctat tgacctggtg agcgcgtcga aggcggagat
     3601 gtccaaacgc tatggcttta tctatgtcga tcgcgacgat gacggcaacg gcaccctggc
     3661 gcgcagtcgc aagaagagct tctggtggta taaagaggtc attgccacta acggcggcag
     3721 cttaaaagag taatcgtctg gaggttgcgt cgctaaccgc cacccggcag aaatgcgcga
     3781 cagtagctaa aaccctcgcc cggataacca ccggcaattt tcgtattacg ccgttacgcg
     3841 cgcgcagcaa
//
