LOCUS       AF135399                5311 bp    DNA     linear   BCT 20-MAR-2000
DEFINITION  Thermus thermophilus strain TH125 AidB, alpha-galactosidase (agaT),
            and galactose-1-phosphate uridylyltransferase (galT) genes, complete
            cds.
ACCESSION   AF135399
VERSION     AF135399.1
KEYWORDS    .
SOURCE      Thermus thermophilus
  ORGANISM  Thermus thermophilus
            Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae;
            Thermus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Fridjonsson,O., Watzlawick,H. and Mattes,R.
  TITLE     The structure of the alpha-galactosidase gene loci in Thermus
            brockianus ITI360 and Thermus thermophilus TH125
  JOURNAL   Extremophiles 4 (1), 23-33 (2000)
   PUBMED   10741834
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Fridjonsson,O. and Mattes,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-1999) Universitaet Stuttgart, Institut fuer
            Industrielle Genetik, Allmandring 31, Stuttgart 70569, Germany
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Thermus thermophilus"
                     /mol_type="genomic DNA"
                     /strain="TH125"
                     /db_xref="taxon:274"
                     /note="HB27 derivative; auxotrophic for tryptophan"
     CDS             771..2114
                     /note="adaptive response gene product; similar to AidB of
                     Escherichia coli"
                     /codon_start=1
                     /transl_table=11
                     /product="AidB"
                     /protein_id="AAD32627.1"
                     /translation="MDLVPYGKNHYEADPELRDLLRHFWPGAGDREAELVMHDLDGNRV
                     DRVRLSPVQREVLKRLRPIVRPPYEGGSWHHHFALGYLLADGGLYCILTITHQVAYPIH
                     KYAPGLASWKERVLYGEAFGATWMTEIQGGSDLGANPHRGPQEGEVWRLYGGDKYFASG
                     AGLADVALVTARPEGAPPGPKGLALFLLPRLDREGRLNYRVRRLKRKSGTRAVPSGEVE
                     LEGSERYLIGRAEEGIYYTLETLTVSRLANAIAAMGIAAKALLETRERVRRRQSFGRFL
                     ADHPLVRYDLLDLSVRQAGGLALALAAVEAFDRAWHERPLTARPTTWPRFLSHLAKNRT
                     AEHSAEITRLAMELFGGLGFLEEYAVARWHRESLITPIWEGPSNIQALDLLEAMQKKGA
                     HRPFLAWLEARLKGRGEEAEGPWRPPARPWTASPPSSPRPPSSRPRRP"
     gene            2559..3989
                     /gene="agaT"
     CDS             2559..3989
                     /gene="agaT"
                     /note="AgaT"
                     /codon_start=1
                     /transl_table=11
                     /product="GH36"
                     /protein_id="AAD32628.1"
                     /translation="MRLNLGGAEVFLRAEGLEEAPGGVRLWGREVRVFPPFPAKGFFRH
                     GWQSWSLAAWVDPAQAPTPLLPEARRPQADDPFLLEAGAWWGSGVGALRGPDGRALLLG
                     ALDLGARVLGREDLLLGRYAGEGGAWFLAHGPEEEVFAAYAQLLPRRLSGRPPRVWCSW
                     YSFYTRIGEDLLLRVLDEVAAFSFEVFQIDDGWQRALGDWEPNDRFPRGMAFLAERIRE
                     RGLRPGLWFAPFLVTADSPLFQMRPDWVLRDGEGRPVRAGFNWGRPLYALDAGNEEVVE
                     WAADLVRKALAWGYDYLKLDFLYAAALPGAEGEARYRRAMARLREAAGEAYLLFCGAPV
                     LASLGLADGLRVGPDVAPYWDNEERSFWLADPTGPGLRNALRSTLHRLWLMENVHVDPD
                     VVYFRTRFNLLSPEEMRLQEALAHFTGFKATSDPPSWLLPEEKGRLEAFLAREVPVRRL
                     GPYRFRVGEEEVDYAPLL"
     gene            3973..5022
                     /gene="galT"
     CDS             3973..5022
                     /gene="galT"
                     /note="GalT; putative"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="AAD32629.1"
                     /translation="MPPFYKRLHRKKDGRELFLYGLMPLEGPPLPEDAEPFRPAPHLRY
                     HPLRGEWVVYAAHRQGRTFLPPKEHCPLCPSREGGFPTEIPFPSFQVAVFENRFPSFVE
                     EAPPPPEGLPVAAGKALGRCEVVVYTPAHTGSLATLTEEERLLLAWAWRDRYEALYALP
                     GVRFVMPFENGGEAVGVTLHHPHGQIYAYPFVPPVLERESQAFRERPVLLELFPNLGPY
                     VVDEEEGFLAFVPPFARYPFEVWVAPVERHPGPWTFSEGEMAAFARLLGRVVARYDALF
                     GEPFPYVMVFHAAPLGEEATFHFHVEFYPPRRTRDKLKFLAGTELGAGTFVVDALPEET
                     AKALRAALP"
ORIGIN
        1 ggatccgtag tcgtagactt tggccgtccc cgaaggaaag gagggccgtt tcgccgccca
       61 cgactcgtcg gcccaggtag ttcaggattt ggacggcctg ggtgcgggag ccgctgatgg
      121 cggtctggcg gatgctggag agcaggacgg cgttcatggc gaccaggacg aggctcagga
      181 cggcgatggc cacgaggacc tctaccaggg tgagccccag ggccttcttc acggcaccac
      241 ctccgtttcc ccgatcaggg agaccgtata ggtgtaggtc ttggacccca ccgtcgcggt
      301 gaagctcagg ggctggcgga ggtttctccc gtcctggtcc gccaccttgc cgggaggcgt
      361 aaaccgggcg accacgcccg gcgttaggct tagagaaacc cccgggggga ggtccaaggc
      421 gcggagagcc gtaccgtccg tccgcctcac ctcgaggcgt tgccccgtga agtggaggac
      481 gaggttttcc cctctgcttg ccgcctcggt ggccgcctcc cagaacaagc cttgcaggga
      541 gcgcaaaaag gcggcctgct cctggcttag gagcgtgcgc cgtgcgttga aggcccctat
      601 ggcgaagatg acggccagta cggcgaggac caccagaagc tctatcacgc tgaaaccctt
      661 ccgcaagctc tccctccctc cttacatggt tggggatttt cgcagggggc gcatccccca
      721 aatgggggag gggcctgtat aatgcccctg catcaggagg aggtagtgga gtggatctcg
      781 taccctacgg caagaaccac tacgaggcgg acccggagct tagggacctc ctccgccact
      841 tctggcccgg ggcgggggat agggaggcgg agctcgtcat gcacgacctg gacgggaacc
      901 gggtggaccg ggtgcgcctg tcgcccgtcc agcgggaagt gctcaagcgc ctgcgcccga
      961 tcgtccgccc gccctacgag ggagggagct ggcaccacca cttcgccctg ggctaccttt
     1021 tggctgacgg cgggctctac tgcatcctca ccatcaccca ccaggtggcc taccccatcc
     1081 acaagtacgc ccccgggctc gcctcttgga aggagcgggt cctctacggg gaggcctttg
     1141 gggccacctg gatgacggag atccaggggg gaagcgacct cggggcgaac ccgcaccgtg
     1201 gcccgcagga aggggaggtc tggcgccttt acggggggga caagtacttc gcctcggggg
     1261 cggggcttgc cgatgtggcc ctggtgacgg cgcggcccga gggggcgccc cctggcccca
     1321 aggggctggc cctcttcctc ctgcccaggc tggaccgcga ggggcgcctg aactaccgag
     1381 tgcgccgcct caagcggaaa agcggtaccc gggccgtgcc ctcgggggag gtggagctgg
     1441 aggggagcga acgctacctc atcggccggg cggaggaggg gatctactac accctggaga
     1501 ccctcaccgt ctcccgcctg gccaacgcca tcgccgccat gggaattgcc gccaaggccc
     1561 tcttggagac gcgggagcgg gtgcggcggc ggcagagctt cgggaggttc ctcgcggacc
     1621 atcccctggt gcgctacgac ctcctggacc tctccgtgcg ccaggcgggg gggctggcct
     1681 tggccctggc ggcggtggag gcctttgacc gggcctggca cgagaggccc cttacggcga
     1741 ggcctaccac ctggccccgc ttcctctccc acctggcgaa gaaccgcacc gccgagcaca
     1801 gcgccgagat cacccgcctg gccatggagc tcttcggggg gctgggcttc ctggaggagt
     1861 acgccgtggc ccgctggcac cgggagtccc tgatcacccc catctgggag gggccgagca
     1921 acatccaggc cctggacctc ctggaggcca tgcagaagaa gggggcccat aggccctttc
     1981 tcgcctggct ggaggcgcgt ctgaagggac ggggcgagga ggccgagggg ccttggcggc
     2041 cgcccgcaag accctggacc gcctcgccgc cctcgagccc gaggccgccc agttctcggc
     2101 caagacggcc ctgaggggcc tggcggacgc tgcggcggtg gcgggcctcc tcgaggcgag
     2161 cgccaccgcc gggccccgct accgggagct cgccgggctt tacgcccgcc gcttcctcct
     2221 cggggaggac tacccgcccg aggccctggg cgcgcgggcg ttgtacctgc ccgagggcgg
     2281 ggcgtgaggc cgcttccctt cgccctcgcc tggggcctcg gcctcggcgg ggtcctcttc
     2341 ctgccgccca tccctgaggg gtggggaatg tcccgcctcg aggccctggc cgccctcccc
     2401 tgggttctgg cgcaggcgcc tgccctgagg gccttggtag cttggggata tggacggctt
     2461 ggcctgggag aggcttcgca tccggatagg ggagaggggt gcccgcgtgg ggcctcgagg
     2521 ccaggccggg tggggtacct gggcggcaag gagggaggat gaggctgaac ctaggaggag
     2581 cggaggtttt cctcagggcg gagggcctgg aggaggcccc ggggggagtg cgcctttggg
     2641 gcagggaggt gcgggtcttt ccccctttcc ccgccaaggg gttcttccgc cacggctggc
     2701 agagctggag cctcgccgcc tgggtggacc ccgctcaggc ccccacgccc ctcctccccg
     2761 aggcgcgccg cccccaggcg gatgacccct tcctcctgga ggccggggcg tggtggggaa
     2821 gcggcgtggg ggcccttagg gggccggacg ggagggcgct ccttctcggc gccctagacc
     2881 ttggggccag ggtcctgggc cgggaggacc tcctcctcgg ccgctacgcc ggagagggcg
     2941 gggcctggtt cctcgcccac ggcccggagg aggaggtctt cgccgcctac gcccagcttc
     3001 tccccaggcg cctttccggg aggcccccca gggtgtggtg ctcctggtac agcttctaca
     3061 cgaggattgg cgaggacctc cttcttcggg ttctggacga ggtggcggcc ttctcctttg
     3121 aggtcttcca gatagacgac ggctggcagc gggccctcgg ggactgggag cccaacgacc
     3181 gcttcccccg ggggatggcc ttcctcgcgg agaggatccg ggaaaggggc ctgaggccgg
     3241 ggctttggtt cgcccccttc ctggtcacgg cggatagccc cctcttccaa atgcggcccg
     3301 actgggtgct gagggacggg gaggggaggc ccgtgcgggc gggcttcaac tggggcaggc
     3361 ccctctacgc cctggacgcg gggaacgagg aggtggtgga gtgggccgcg gacctcgtgc
     3421 gtaaggccct cgcctggggc tacgactacc tgaagctgga cttcctctac gccgccgccc
     3481 ttcccggggc cgagggggag gcccgctacc ggagggccat ggcccgcctg cgggaggcgg
     3541 cgggggaggc ctacctcctc ttctgcgggg cccccgtcct cgcctccctg ggcctcgccg
     3601 acggcctccg ggtaggcccc gacgtggccc cctactggga caacgaggag cgctcctttt
     3661 ggcttgcgga ccccacgggg ccgggcctaa ggaacgccct ccgctccacc ctccaccgcc
     3721 tctggctcat ggagaacgtc catgtggacc ccgatgtggt ctatttccgc acccgcttta
     3781 acctgctttc ccccgaggag atgcggcttc aggaggcctt ggcccacttc accggcttca
     3841 aggccacttc cgacccgccc tcgtggctcc tcccggagga aaaggggcgc ctcgaggcct
     3901 tcctcgcccg ggaggtcccg gtgcggcgcc tcggccccta ccgctttagg gtaggggagg
     3961 aggaggtgga ctatgccccc cttctataag cgcctccacc ggaagaagga cggccgggag
     4021 ctcttcctct acggcctaat gcccctcgag ggcccccctc tccccgaaga cgccgagccc
     4081 ttccgccccg ccccccacct ccgctaccac cccttgcggg gggagtgggt ggtctacgcc
     4141 gcccaccgcc aggggcgcac cttcctcccc cccaaggagc actgcccctt gtgcccgagc
     4201 cgggaagggg gcttccccac ggagatcccc ttcccctcct ttcaggtggc cgtctttgaa
     4261 aaccgcttcc cctccttcgt ggaggaagcc cctccccctc ccgaggggct tcccgtggcg
     4321 gcggggaagg ccttggggcg gtgcgaggtg gtggtctaca cccccgccca cacgggaagc
     4381 ctcgccaccc tcacggagga ggaaaggctc cttttggcct gggcgtggcg ggaccggtac
     4441 gaggccctct acgcccttcc cggggtgcgc ttcgtcatgc cctttgagaa cgggggggag
     4501 gcggtggggg tcaccctcca ccacccccac ggccagatct acgcctaccc cttcgtgccc
     4561 cccgtcctgg agcgggaaag ccaggccttc cgggaaaggc ccgtgctcct tgagcttttc
     4621 ccgaaccttg ggccctacgt ggtggacgag gaggagggct tcctcgcctt cgtccccccc
     4681 ttcgcccggt acccctttga ggtctgggtg gcccccgtgg aaaggcaccc cggtccctgg
     4741 accttctccg agggggagat ggcggccttc gccaggctct tgggccgggt ggtggcccgg
     4801 tacgacgccc tctttgggga gcccttccct tacgtcatgg tcttccacgc cgcgcccttg
     4861 ggggaggagg ccacctttca cttccacgtg gagttctacc ccccgaggcg cacccgggac
     4921 aagctcaaat tcctggcggg gacggagctc ggggcgggga cgttcgtggt ggacgccctg
     4981 cccgaggaga ccgcgaaggc ccttagggcg gcgttgccgt gacggccaaa gcccaggcgt
     5041 ggggcgtcct ggggtgggga ctttgcccta gggtacaaac ccgttcttcg tgaaacatag
     5101 aatgcaaagg gtgaaagcgg ctccgaaggc gctcctcccg tggctcgcgt tgctcagcgc
     5161 cctggcgaca gtcgcggtaa acgccgccgc caacgccctt cccctgaacg gccttaagac
     5221 cggggagatc tccgaccggt ttgcggtgta cttcgtgatg caccggcggg gtacgtcttt
     5281 gcggtgtggg gcctgattta cgtggggatc c
//
