LOCUS       KU644713               16598 bp    DNA     linear   BCT 31-MAR-2016
DEFINITION  Bacillus sp. N16-5 galactomannan utilization gene cluster, complete
            sequence.
ACCESSION   KU644713
VERSION     KU644713.1
KEYWORDS    .
SOURCE      Bacillus sp. N16-5
  ORGANISM  Bacillus sp. N16-5
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Song,Y., Li,J., Meng,S., Yin,L., Xue,Y. and Ma,Y.
  TITLE     A Novel Manno-Oligosaccharide Binding Protein Identified in
            Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
  JOURNAL   PLoS ONE 11 (3), E0150059 (2016)
   PUBMED   26978267
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16598)
  AUTHORS   Song,Y., Xue,Y. and Ma,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2016) State Key Laboratory of Microbial Resources,
            Institute of Microbiology, Chinese Academy of Sciences, No. 1 West
            Beichen Road, Chaoyang District, Beijing 100101, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. version 7.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Bacillus sp. N16-5"
                     /mol_type="genomic DNA"
                     /strain="N16-5"
                     /isolation_source="sediment of Wudunur Soda Lake"
                     /db_xref="taxon:122631"
                     /country="China: Inner Mongolia"
     misc_feature    1..16598
                     /note="galactomannan utilization gene cluster"
     CDS             complement(1..1020)
                     /codon_start=1
                     /transl_table=11
                     /product="GH130"
                     /protein_id="AML27054.1"
                     /translation="MTVKLIGEQLTNIPWEDKPEGLTMPVWRHTANPVIKRNPAKGIAR
                     IFNSAVIPYDNKFLGVFRAETTNGRPHLHLGYSDDALNWEIDEQKMTFVDDSGAEFQPR
                     YAYDPRLIKVDNTYYIIWCTDFYGASIGMAKTHDFKTFIRVENPFLPFNRNGVLFPRKI
                     NGNYLLLSRPSDSGHTPFGDIFLSESPDLTYWGKHRRVMGKGGEGWWQNVKIGGGPAPI
                     ETSEGWLIFYHGVTGTCNGLVYSMGGAILDLHEPSKVKYRCKTFLLTPETEYEERGFVN
                     NVIFPCATLSDAQTGRIAIYYGAADTYVGVAYTEVNQVVDYIKEHHEQVEDDQEIGTM"
     CDS             complement(1300..2304)
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="AML27055.1"
                     /translation="MRDIADKIGVSSVTVSKALNDKEGVSEELRKKIQSLAEELGYRFN
                     STAKSMKEGLSYNIGVIIPEHFIGETKSFYLSFYQEITKHLESYHYYGILQTLSQEDEM
                     NHVLPGTYYENKVDGFIILGQISNEYLNVLKNNGIPLICLDFYTDQTEVDCIITDNFYG
                     TYEVTNHLIKMGHREIAFVGNIYFTSSIQDRYLGYYKSLLEHRLPLKEHFIINDRDDNG
                     TFIDIQLPKERPTAFVCNCDQVAYNVVKKLQRLGYRIPEDFSVVGFDNDIYSTIASPQL
                     TTVQVNVPEMSEAAVRNIIEKVRDNQKASGRVLVKGKIVQRDSVADINSKLKK"
     CDS             complement(2366..3541)
                     /codon_start=1
                     /transl_table=11
                     /product="GH130"
                     /protein_id="AML27056.1"
                     /translation="MIHDHYYEMLEKQEKLLSKPNKVNDKFYNGIYEKYKNPVLTRHHV
                     PLHWRFDLNKETNPYFMERLGINATFNPGAIYWNHRYYLVVRTEGVDRKSFFALAVSDT
                     GIDNFRFIDSPLTWERIDEETNMYDMRLVEHEDGWVYGIYCSESKDPSAEPFDTSSAVA
                     QAGLVRTKDLKTWERLPNIKTPSPQQRNVVLHPEFINGKYALYTRPQDGFISTGAGGGI
                     AFGLCDSLENPVIDHETVIDEKVYHTVYEVKNGQGPAPIKTPKGWIHIAHGVRNTAAGL
                     RYVLYTFATQLDAPHKILAKPGGHFIAPYDDERTGDVSNVIFCNGAVLNKNGEIFIYYA
                     SSDTRIHIAKTTIDKLTDYTFNTPEDPYNSLECVRQRMDLIEKNLHLLKGK"
     CDS             complement(3777..5084)
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="AML27057.1"
                     /translation="MVTGLVVPLVFLAACGDENTGGNNDGIVKDEEANNGEVAGDITVL
                     IHRTDIVDTAFMEYKERFNETYPDVTVTFEAITDYEGQVSTRMNTDDYGDVLMIPDNVP
                     IEDAPHFFRPLGNTEELSEIYLFADEFAYEGRTYGLPITINSDGILYNQAVFEEAEINS
                     IPATPEEFISALQMIKDNTDAIPLYTNYGDSWPLDQWETNRLAIAGDEQFTNDLPHNKT
                     PFAEGEPHYILYNLMYEVANQGLIEGDPLTTDWELSKQMLADGDVATMVLGSWSYAQVE
                     ELAENPEDIQFMPFPYTHEDGTVYATVGPDFNIGINANTDYPAAAEAWVKFFLDESGYW
                     EDEGGISPKASDEFPDFLEAYEELGVEFISNAPARDGEQGLADRVDSSGEVGLWNSRFK
                     EQIIEAGIGNRDESFDDIMNQLNERWQDGRSLEGIE"
     CDS             complement(5855..7060)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AML27058.1"
                     /translation="MKSNYNPLYFLAALGNGGLSVSFFMYLMFMVPHPDTPMATFNHIY
                     PYITEGFSIVSVSIVLALVAMIYFAVKHFGKVIWNVKQFNQFKQTEAYNTLRSGNGEVS
                     LMAIPLTFSMMINVVFVLGAVFVPGLWDYVQLLLPFSLLGFMIVGYFALKIFVRMMTRF
                     MTTSSFKEEANNHFSQLLSVFAFTMIAVGFAAPGAMSHNMVVSAAGILFSILFTVLSFI
                     LIVVFIGLGLQSVFKNGLSLEASPSVWMLIPILTLIGITGVRITSGLSHNFLETNPHPL
                     IFFVGLGMIMTSQFVVGVLGYSTLKKIGYFNNFVGGEKKSAGSFGLICPGVAFFVLGMF
                     FAHLGLVQNGIVDKFSVSYFIILAPFVLVQFKTIQALSKLNKKHFSSFEVTKTNVQEQT
                     LS"
     CDS             complement(8027..9196)
                     /codon_start=1
                     /transl_table=11
                     /product="GH27"
                     /protein_id="AML27059.1"
                     /translation="MTLKPKRYAEGLAKTPPMGWNSFNTFGCEPTEELIKQSADVMVKS
                     GLLEAGYRYINIDDGWMADERDSAGNLVPDPQKFPNGMKPVTDYIHEKGLLAGTYLGCG
                     QKTYGEKPGSLGYEERDAQLIADQGFDLLKYDYRELPGDPIGRGVKEDYVTMRNALMKT
                     GRDMVFSICEHGKSHPETWAQEIGHMWRTTPDIKDSFDEDINWGWSINHIIDETHALHH
                     YAGPGGWNDPDMLVVGINGLNDWLGPGCTYNEYKSHFSLWCLLAAPLLIGCDIRKMSEE
                     TKTILLNKEMIAINQDPLGIQGHLLKKEHGIDYWVKPLANNDIAIGLLNRFNEPKEAVL
                     SLSDLLEDGNYLMKDVWTNERKELQSEWISKTLKSHECAVFRLMSKNKE"
                     /old_product="alpha-galactosidase"
     CDS             complement(9550..10353)
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8DT26|3.A.1.1.27"
                     /protein_id="AML27060.1"
                     /translation="MKYFWAVMKYLSLILGVLITAIPIIVVLFASFKTREEYATTGPLS
                     PPESWMNVENYVRAFTEGNMLLGFANTAFMLVVSVFFAILFGAMIAYILSRFKFRGSKV
                     LLGLFLLATLIPAVTTQVATFQIISGLGLFNTRWSAIVLFMSTDIIAVYIFLQFLDRVS
                     ISLDESAMLDGASYFTIFRKIILPQLKPAMATVLIIRGVTIYNDFYIPFLYMPRPDXLF
                     RFQGPYGSQWEVICAGVIIVIIPTLIVFVLLQKYIYNGFTEGSVK"
     CDS             complement(10356..11297)
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P94529|3.A.1.1.34"
                     /protein_id="AML27061.1"
                     /translation="MEGKRMAQSVYQKSTKISKLLSSRNFNYKKQKMLIIVLFSLIPVT
                     LLLTFSYLPLVNMIIYSFHNWNGISPNKEWVGFHNYVLMFTRPEYFAVFVVSLYYFFAT
                     FIQMGIALYFATVLSFNVRFKNFFKGVIFFPYLLNGVAIGFIFLFFFRPEGTLDSLLSL
                     IGLGEYTQLWLGNPDLINISLAGTSVWRYMGFNFIIFLGAISSISKEIYEAADIDGANS
                     WQKFRFIILPSIKRIVELNLILAISGAIGVFEIPFIMTGGANGSTTFVIQTMTTAFEYN
                     RIGLASAMAVVLLIIIIIVTLVQRLLLRDKEA"
     CDS             complement(11364..12308)
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-1,4-mannanase"
                     /protein_id="AML27062.1"
                     /translation="MDYIKGMTWGWIGNSEDWRSNEAERSMEEMTNLAINWTAIAFQGL
                     QETAHSPDITFAEPPMVTDENVRWAIAKAKSLGLSVILKPIVNVRDGTWRAHINFFDKD
                     VPCEPTWSQWFKSYESFMLHYAKLAEDTGCEMLCIGCEMVQTERREKEWRDLIQKVRQV
                     YSGIITYNCDKYQEDEVTWWDAVDVMSSSGYYPIGSWEHHESRIKKIVESWQKPFFFME
                     AGCPSRLESGSVPNDWNKNRGQIDMDEQRVFYEEMFKFFHGQKWFYGFMLWDWPAKLYR
                     LEDASENDDYCVYGKPAAEVIKSFFTSNKIAKR"
     CDS             complement(12330..13544)
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylglucosamine 2-epimerase"
                     /protein_id="AML27063.1"
                     /translation="MKELEKQRKLDELSQELKNELVANILPYWVTHVMDKTNGGFYGYL
                     TTHNKVDEKAPKGGILNSRILWTFSKAYHVFGNRHYLETANHAYVYLRDFFLDKEDGGV
                     YWMVDHKGKPQETRKHIYNQSFAIYALAEYFKITCHEESLQIAKDIFYLIEKYGYDRQY
                     KGYLEAFTKEWKMEQDPRLSESDMEAEKSMNTHLHILEAYTNLFKVWKSPQLKERITEL
                     IDVMLKHIVGPTDQFTLFFDRQWHGMANIVSYGHDIEGSWLLYEAAEVLGDEKRLEKVR
                     RTSIDMAEKVAIEGFSESGGLINEREGDDVDEEKVWWVQAEGMIGFFNAYQLTLSNDYL
                     EAVYKLWRFTKQHMVDKHYGEWYWKLDEQNNAYLDRPKVEPWKCPYHNSRCCFELIERI
                     DRCKS"
     CDS             complement(13792..14748)
                     /codon_start=1
                     /transl_table=11
                     /product="CE7"
                     /protein_id="AML27064.1"
                     /translation="MPTIDLPLSELKVYKGTNPKPVDFDDYWERGLSEMRAVDPQVHFK
                     KSDFQVPFATCYDMYFTGVKGARIHVKYVKPVSVSDPVPAVLMFHGYSMNAGDWSSKLA
                     YAALGHAVFTMDVRGQGGESEDVGGVKGNTLKGHIIRGLDDDKDNLFFRDVYLDCAQLA
                     QIVIGMDEIDGANVSATGWSQGGALTLVCASLEPRIKKAAAVYPFLSDFKRVWELDLAI
                     DAYEDIKHYFRRFDPQHKRVEEVFTTLGYIDIQHLAERIKAKTLMATGLMDTICPPSTQ
                     FAAFNRIKSEKQLEIYPDFSHENLPGLHDIIMQYLRS"
     CDS             15117..16598
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-mannosidase"
                     /protein_id="AML27065.1"
                     /translation="MKKKLSQIYHLIICTLIISVGIMGITTSPSEASSGFYVDGNTLYD
                     ANGQPFVMKGINHGHAWYKDTASTAIPAIAEQGANTIRIVLSDGGQWEKDDIDTVREVI
                     ELAEQNKMVAVVEVHDATGRDSRSDLDRAVDYWIEMKDALIGKEDTVIINIANEWYGSW
                     DGAAWADGYIDVIPKLRDAGLTHTLMVDAAGWGQYPQSIHDYGQDVFNADPLKNTIFSI
                     HMYEYAGGDANTVRSNIDRVIDQDLALVIGEFGHRHTDGDVDEDTILSYSEETGTGWLA
                     WSWKGNSAEWDYLDLSEDWAGNHLTDWGNRIVHGANGLQETSKPSTVFTDDNGGAPEPP
                     TTTTLYDFEGSTQGWHGSNVMGGPWSVTEWGASGNYSLKGDVNLSSNSSHELYSEQSRN
                     LHGYSQLNATVRHANWGNPGNGMNARLYVKTGSDYTWYSGPFTRINSSNSGTTLSFDLN
                     NIENSHHVREIGVQFSAADNSSGQTALYVDNVTLR"
ORIGIN
        1 tcacattgtc cctatttctt gatcatcctc cacttgttca tggtgttctt ttatataatc
       61 tactacctga tttacttctg tgtaagcgac ccctacataa gtgtcagcag ccccataata
      121 aatagcaata cgccctgttt gtgcatcact caatgtagcg catgggaaga taacattatt
      181 gacaaagcct ctttcttcat actctgtttc aggggtcaac aaaaatgttt tacaccggta
      241 ctttaccttt gatggttcgt gtaaatcaag aatcgcgccg cccatactat atactaggcc
      301 attacaagtg cctgttaccc catgataaaa tataagccat ccttcagatg tttcaatagg
      361 cgctggtccg cctccaattt taacattttg ccaccagcct tctccacctt ttcccatcac
      421 acggcgatgt ttcccccaat acgttaaatc cgggctttca cttaaaaaga tatctccaaa
      481 aggcgtatga ccgctatcac ttggacgtga taataacaga taattaccat taatctttct
      541 tggaaatagt acgccattac gattaaatgg tagaaatgga ttttcaactc ttataaacgt
      601 cttaaagtcg tgcgtttttg ccatgccgat agaagcacca taaaaatccg tacaccaaat
      661 aatatagtac gtattatcaa ctttaatgag tctcggatca tacgcatatc tcggttgaaa
      721 ttcagctccg gaatcatcaa caaaggtcat tttctgttcg tcgatttccc aatttaaagc
      781 gtcatcactg taacctaaat gcagatgagg tctgccattt gttgtttcag ccctaaatac
      841 accaagaaat ttattatcgt aagggatgac ggcactatta aatattctag caatcccttt
      901 tgcaggatta cgtttaatta caggattagc cgtatgacgc caaactggca ttgtcagtcc
      961 ctctggcttg tcttcccagg gtatgtttgt tagctgttcc ccaattaatt taactgtcat
     1021 tcatcaaact cctctacttt ttagccaata gctaattttt agtttatcgt taaggttata
     1081 atataaagat accttagaag cgctttcatg tcaattttct tttctgtatt tatcagtttt
     1141 caagcttcac ttttactttg atctgaacta gattgcactt aaaaccaact tagtttcgac
     1201 actatcatcc gcccactcac ccttttctaa ttacatacaa aaaaaccaac aatggctata
     1261 tcgtcgattg ttggttttca tctttaccgc atgcatgttt tattttttta attttgaatt
     1321 gatatcagca actgaatccc tttgaacaat ttttcctttc actaatactc ttcctgatgc
     1381 tttctgatta tccctgactt tttctataat atttctcacg gcagcttcag acatttccgg
     1441 aacgtttact tgtaccgtcg taagttgtgg tgaagcaatc gtcgaataaa tgtcattatc
     1501 aaagcctact accgaaaaat cttcaggtat ccggtaacct agacgttgca attttttaac
     1561 gacattgtaa gccacttgat cgcaattaca tacgaaagct gtcggtctct ctttaggtag
     1621 ctgaatgtct ataaacgtcc cattatcatc acggtcattt atgataaaat gctcttttaa
     1681 ggggagacga tgctcaagta gcgatttata atagcctaaa tagcggtcct gtatactact
     1741 cgtaaaataa atatttccaa cgaatgcgat ctctctatgt cccatcttga ttaaatggtt
     1801 tgttacttcg taagtgccat agaagttatc cgtaataata caatcaactt cagtttgatc
     1861 cgtataaaag tccaggcaaa taagtggaat accattattt tttaacacat taagatactc
     1921 attactaatc tggccaagaa tgataaagcc atctacttta ttttcgtagt atgtcccagg
     1981 aaggacatgg ttcatttcat cttcttgtga gagcgtttgt aaaatcccat aataatgata
     2041 actttcaagg tgtttggtaa tctcttgata aaagcttaaa taaaaggatt tcgtttcccc
     2101 aataaagtgt tccgggatga tgaccccaat attataggat aagccttcct tcatagattt
     2161 agccgttgaa ttaaatctat aacctaattc ttcagccaaa ctttggattt tttttcttaa
     2221 ttcttcactc acgccttctt tatcatttaa agctttagag accgtgacac tactcacacc
     2281 aattttgtca gcaatgtccc tcatcgtcac attgttccgc aactaagcca cctcctcaca
     2341 acacaggtat actataaata tgatactact ttcctttaag taagtgtagg tttttttcta
     2401 tcagatccat tcgctgtctc acacactcta gcgagttata cgggtcttct ggcgtgttaa
     2461 acgtataatc tgttaattta tcaatcgttg ttttggctat atgaatacgc gtatcactcg
     2521 atgcatagta tatgaagatt tccccatttt tattcaagac agccccattg cagaaaatca
     2581 catttgatac gtcccccgtt ctttcatcgt cataaggtgc gatgaaatgg cctcccggtt
     2641 tggcaagtat tttatgcggg gcatccagtt gagtagcgaa tgtgtagaga acataccgta
     2701 atccagcagc tgtattcctc acaccatgag ctatatgaat ccagccttta ggtgtcttta
     2761 tcggtgctgg cccttgacca ttttttactt cataaaccgt atgatacact ttctcatcga
     2821 tcactgtttc atgatcaatt acaggatttt ctagtgagtc acataagcca aatgcaattc
     2881 ctcctcccgc tccagtggaa ataaatccat cttgtggtcg cgtatataat gcatatttcc
     2941 cattaataaa ctctggatgg aggacaacat ttctctgttg tggtgatggc gttttaatat
     3001 tcggcagacg ctcccatgtt tttaaatctt ttgttctcac gagtccagct tgggctacgg
     3061 cacttgacgt gtcgaatggc tctgctgatg ggtctttact ttctgagcaa taaattccat
     3121 agacccagcc gtcttcatgt tcaacaagtc gcatatcata catattcgtt tcttcatcta
     3181 tcctttccca tgttaaaggt gaatctatga atctaaaatt atcaatgccc gtgtcactta
     3241 ccgctagagc aaaaaatgat tttctgtcta ccccttctgt cctaacgact aagtaatagc
     3301 gatgattcca atagatagct ccaggattaa aggtggcatt aattcctaaa cgttccataa
     3361 aatacggatt cgtttcttta tttaaatcga acctccagtg caaaggaaca tgatgtcttg
     3421 taagtacagg atttttatac ttttcataaa tcccattata aaacttatca ttaactttat
     3481 ttggcttgga aagcagtttc tcttgctttt ctaacatctc ataatagtga tcatgtatca
     3541 tctaattcac ctcttcgttt tctattactc tatcttgact actgaaagac tctactaacg
     3601 atatattcca cctttccttt gtagtaaagg tgctcacctc tcatgtgtac ctacttttat
     3661 ccattaaaat gcggccccat ttagaaactt ctgtatctta ataacatgaa tgagaagtcg
     3721 tttaccttgt ggagccgcaa aaagaacaaa gttattgttt aagtttaaat cgacacttat
     3781 tctatacctt caaggcttcg accatcttgc catctttcgt taagttggtt cataatatca
     3841 tcgaatgatt catcgcggtt gccaatgccc gcttcgataa tttgctcttt aaaccttgaa
     3901 ttccataaac ccacttcccc cgaactatcc acgcgatctg ccaagccctg ttccccgtca
     3961 cgagcaggtg cattagaaat gaattctact cctaattctt catatgcttc caagaaatca
     4021 gggaattcat cacttgcttt aggtgaaata ccaccttcat cttcccaata gcctgattca
     4081 tctaggaaaa atttaaccca tgcttccgct gctgcaggat aatctgtatt agcattaata
     4141 ccaatattaa aatccggtcc caccgtcgca tagacggttc catcctcatg agtgtaaggg
     4201 aatggcataa attgaatgtc ttcagggttc tctgctaatt cttctacttg agcatatgac
     4261 catgagccga gaaccatagt tgcaacatcg ccatctgcaa gcatttgttt agaaagttcc
     4321 cagtccgtgg tgagggggtc accttcgata agtccctgat tagcgacttc atacatgagg
     4381 ttataaagaa tatagtgtgg ttctccttcc gcaaaaggtg ttttattatg tggaaggtca
     4441 ttggtaaact gttcatcccc agcaatggca agccggttcg tctcccattg gtctaatggc
     4501 caactgtcac cataatttgt atagagtgga atggcgtcag tgttatcttt aatcatttgc
     4561 agggcagata taaactcttc gggtgttgct ggtatactat taatctctgc ttcttcgaaa
     4621 acagcttgat tgtagagaat gccatcagag ttaatcgtaa taggaaggcc gtacgttcta
     4681 ccttcatatg caaattcatc tgcaaataaa tatatctcac ttaactcttc tgtatttccc
     4741 agtggtctaa aaaaatgagg cgcatcttca attggtacat tatcaggaat catgagtaca
     4801 tccccataat catctgtatt catacgagta ctaacttgac cttcataatc ggtaatagct
     4861 tcgaacgtca cagtaacatc tggataggtt tcattaaaac gctctttata ttccataaaa
     4921 gcagtatcta cgatgtctgt tcggtgaatt aatacggtta tatctccagc cacttcacca
     4981 ttattcgctt cttcatcctt cactattccg tcattattac cgccagtatt ctcatctcca
     5041 cacgcggcca aaaagactaa aggcacgact aatcctgtaa ccaaccacaa ttttttgttc
     5101 attttctttc cccctttaaa attagttaat cggtaaagta acaacaagtc aagattaata
     5161 tgtaatcgct ttcttgtcaa tggatgtaag gaaaatagtg aataaaaaga ggtttgcatc
     5221 gcttactata ttggtttgat cagtgttatt aaattaaaac atggaacgag tttgatacag
     5281 gcaaatagcg ataaacttaa tctcatagat gacatacaaa aatgacccga aaacgaattc
     5341 aggtcatgag ctcactactc tttatttatt tttttgattt aaccctggcg cattcttggc
     5401 ttaatagtgt ttgatataaa acatactgtt tttcgattct tcagcaaacc tatataccaa
     5461 catcgttttc attttcatag taatcaaatc tccttttcgg tcatattttt ttgctttata
     5521 caaacgttca gccttagctt agggggacaa aatcttccag cttgccccag cgattatgtc
     5581 gattcaactg cccgctgcat atcatcaatt tcaccaacaa tcagttcctc cagcatctac
     5641 acaagcaaaa gttgaagtag cttcaatatg aaataaccgt atatctgact taaaaatcat
     5701 aatatacggt tatttaccat gcgtgccgtg aagatcggct tattttttcg tgcttacttc
     5761 ttaacatatt ttattcgtct atagagttag acattttaat tatgtcccaa cctctcaatt
     5821 tattttcgaa aggaatattg aacccctttt taacctaact aagtgtttgc tcttgaacat
     5881 ttgtttttgt tacttcaaat gatgaaaagt gtttcttatt taatttagat aaagcttgaa
     5941 tcgttttaaa ctgtacaaga acgaatggcg caaggatgat gaagtatgat actgaaaact
     6001 tatcaacaat gccgttctgg acaagcccca aatgagcaaa gaacatccca agtacgaaga
     6061 aagcaacccc cggacaaatt aatccaaaac tccctgcact tttcttttct cctccaacaa
     6121 agttgttgaa atagccgatt ttctttaacg tagaataccc aagtacacca acaacgaatt
     6181 gtgatgtcat tatcatacct aagccaacaa aaaagataag tggatgtgga tttgtctcta
     6241 aaaagttatg agagagacca gacgtaatac gcaccccagt aataccaatc agagttaata
     6301 tcggtattag catccataca cttgggctag cttctaatga aagaccgttt ttaaagactg
     6361 attgtaaccc taaaccaatg aagacgacga ttaaaataaa acttaagaca gtaaatagaa
     6421 tagagaaaag gatccccgca gctgacacaa ccatgttatg actcattgcc ccaggagctg
     6481 caaatcctac tgcaatcatt gtaaaggcaa aaactgatag caattgactg aaatgattgt
     6541 ttgcctcttc tttgaaagac gatgttgtca taaaacgtgt catcattcga acgaatattt
     6601 ttaaagcgaa atatccaaca atcatgaaac caagcaatga aaatggaagc agaagttgta
     6661 cgtagtccca taaacccggt acaaatactg cccctaaaac aaatacaaca tttatcatca
     6721 tagaaaatgt aagtggaatt gccattaagc taacttcacc attgccagaa cgcaatgtat
     6781 tgtatgcttc ggtttgttta aattgattaa attgctttac attccatata actttcccaa
     6841 aatgttttac tgcaaaataa atcattgcta ctaaggctaa aacaattgaa acagatacaa
     6901 tcgaaaaacc ttctgttata tacggataaa tgtgattaaa cgttgccatt ggtgtatccg
     6961 ggtgtggaac catgaacatt aaatacatga aaaatgagac ggatagccct ccgtttccta
     7021 gagcagctaa aaaatataat ggattgtagt tacttttcat atctttctct cctcattata
     7081 ccctaagggg tattttttgt atggttttat aatatcaaat aaatttgatt ttgtaaacga
     7141 taatctttca agtaaaaaag aatatggcat aattttgtac attgcatata aagatgacgt
     7201 ctttaatttg gtgcaacacc ggaacaacgg tcatattatt tctttaaatt gtgttgaaaa
     7261 aaggagaaag tgttaaaagc actcttcctt aattaataga taatacgatt taatattatt
     7321 actcgacgta ttcaaattat taacccataa caggaacctt caatcaggac attaccgtcc
     7381 gttagctcac gcctatatag atttatctct cctctctatt ttgagtcgga agttttacgg
     7441 acggttatct gtgatcaact ttttcctaat tgaatcgatg tcataaatca ttaatgtata
     7501 gttatttcgg aacgttatga ctcattttca caacagatgg gtgctttctg tggggcttgt
     7561 ggtcagctaa ttcttccatc gtaaagctct gtcagaatgg attttcagac ttcgctttat
     7621 cctctgggag ttgccgtttt acacattgcc tcccttaata ttttaagaga aataatcaaa
     7681 atcattcgtt tatttgtgtg aaaaatgtcc ttatgtaatt gattcacttt tattttaatt
     7741 caaaaaatcc tttataatgg ttgggtggta gtaaaccgat cccaatccaa aataaaggat
     7801 aaatcaatgt ataagttact catttaaaca ctatttttcg cttatttctt ctgatttttt
     7861 acagaaacaa gtgagaaacc atcaattata tctcactata caaagaatct gcatacggat
     7921 tcgtttttgc agttactgct ctatacatag ttacacgaaa ctgaggatta acgtgcgttt
     7981 tctttgaagg attacaacga gcaacgaact tggataccat tagccactac tctttatttt
     8041 ttgacataag cctaaaaacc gcacattcat ggctcttcaa tgttttactt atccattctg
     8101 actgtagctc cttccgttca tttgtccata catctttcat taaatagttg ccgtcttcga
     8161 ggagatcact caggctaaga acagcctctt taggctcatt aaagcgattt agcaagccaa
     8221 tagctatgtc attattcgca agaggtttta cccaataatc aatcccatgt tcttttttaa
     8281 gaagatgacc ttgtatacct aatggatctt gattaatggc aatcatctct ttatttagta
     8341 aaatcgtttt ggtttcctca ctcatttttc gaatatcaca accaataagg agaggggctg
     8401 cgaggagaca ccacaagcta aaatgtgatt tatattcatt atacgtacag ccgggtccaa
     8461 gccaatcgtt taatccatta atccccacga ctaacatatc tggatcgttc caaccacctg
     8521 gccctgcgta gtgatgaagt gcatgggtct catctataat atgattaatt gaccagcccc
     8581 agtttatatc ctcatcaaag ctgtctttaa tatctggggt tgtacgccac atatgaccaa
     8641 tttcctgagc ccacgtttct ggatgtgact tcccatgttc acaaatactg aagaccatgt
     8701 ctctaccagt tttcatcaag gcgttcctca tcgtcacgta atcttctttc acccctcttc
     8761 ctattggatc acctgggagt tcacggtaat catacttcag taaatcaaac ccttggtcag
     8821 caattaattg tgcatctctt tcttcatatc ctaagcttcc tggtttttct ccgtatgttt
     8881 tttggccaca gcctaaatag gtgcctgcta aaagtccttt ttcatggata taatcagtga
     8941 cgggtttcat gccgttcgga aatttttgag gatcaggaac tagattacca gcactatctc
     9001 tctcgtctgc catccacccg tcatcaatgt tgatatagcg gtatcctgct tctaaaagac
     9061 ctgattttac catgacatct gcactctgtt ttatcaactc ttccgttggt tcacacccga
     9121 aagtattaaa tgagttccat cccatcgggg gagttttagc caatccttca gcatatcgtt
     9181 ttggttttaa agtcatgcct tctcctccaa acaaataaaa ttgattagtt tctcttacac
     9241 tttatttctc catggtgata ccacttacgc caaacaattt ctaccttttc cccataaaat
     9301 gaacgttcaa tcagtcgtag ttgagataat caggatatta gtgtccgtta actcccgatt
     9361 aaatagagtt agctctcctt tcaattttga tccgggagtt tcacgaacgc ttatttgtgc
     9421 taaaatgaca atgcgattgg taggctcctc ttttcatatc ccatccatat gtcatctcct
     9481 tcataaataa ttacgccata tcttgtcctc cctactaatc aatgctattc attttataag
     9541 agtctttagc tatttcactg atccctcagt aaatccgtta tatatatatt tttgtaacaa
     9601 aacaaacaca atcagagttg ggataatgac gatgataacg cctgcacaaa ttacttccca
     9661 ttgtgaccca tatgggcctt gaaaacggaa taagngatct gggcgtggca tatataaaaa
     9721 tggaatataa aagtcgttgt aaatcgtgac accgcgaatg attaagaccg ttgccatggc
     9781 aggttttagt tgcggtaaaa taatttttct aaagatagta aaatacgaag caccatctaa
     9841 cattgctgac tcatctagcg atattgaaac tctatctagg aattgcaaga aaatatatac
     9901 cgcgataata tctgtgctca taaaaaggac gatggcagac cacctcgtat taaacaaacc
     9961 taatccacta ataatttgaa acgtggcgac ttgtgttgtc accgctggga ttaaagtagc
    10021 cagtaaaaac aaacctagta acactttact cccgcgaaat ttaaagcggc ttaagatata
    10081 agcaatcatt gcgccaaaaa gaatcgcaaa aaagacggaa acaacgagca taaatgctgt
    10141 atttgcaaaa ccaagaagca tatttccttc agtaaacgca cggacatagt tttcaacatt
    10201 catccaactt tctggtggag aaagcgggcc tgttgtggca tattcttccc gtgttttaaa
    10261 agaggcaaaa aggacgacaa tgatgggtat tgcagtaatt aacacaccaa gtatgagtga
    10321 caaatacttc ataactgccc aaaaatattt cattgtcacg cctccttgtc tcttaacagt
    10381 aatcgttgta ccaacgtcac gataatgata ataattaaca ggacaaccgc catggctgat
    10441 gcaagtccaa tacggttata ttcaaatgct gttgtcatcg tttggatcac aaatgtcgta
    10501 ctgccattag caccacctgt cataataaat ggaatttcaa acacaccgat agcaccacta
    10561 atcgcaagta ttagattaag ctcgacaatt ctcttaatac taggtagaat aatgaatcta
    10621 aatttttgcc aactatttgc accatcaata tctgctgctt catatatctc ttttgaaata
    10681 gacgaaatgg ccccgagaaa aatgatgaaa ttaaacccca tgtaacgcca cacggaagtt
    10741 cctgctaatg atatattgat gagatcagga tttccaagcc aaagttgcgt atattcccct
    10801 aaacctatca aactaagtaa tgaatctagc gtcccttctg gtcgaaagaa aaatagaaaa
    10861 ataaaaccaa tggcgacgcc attcaataaa taagggaaaa atataacgcc tttgaaaaaa
    10921 tttttgaacc tgacattaaa actcaaaacc gttgcaaaat ataacgcgat gcccatctga
    10981 ataaaggtgg caaaaaagta atataaactg acgacaaata cagcaaaata ttctggtctc
    11041 gtaaacatga ggacatagtt gtgaaaaccg acccactctt tgttcggact aatcccgttc
    11101 cagttatgga agctataaat gatcatatta actaaaggta agtaagaaaa tgttaatagt
    11161 aatgtcacag gaataagtga aaacaaaaca ataattagca ttttttgttt tttataatta
    11221 aaatttctag aggacaataa cttggagatt tttgtagact tttgataaac ggattgagcc
    11281 attcttttcc cctccatgta aacacctttt tatcatgtcg aagcgtcgtt ttatatcaag
    11341 ttgccaatga ctagccgtgt tttctatctc ttagctattt tatttgaggt aaaaaatgat
    11401 ttaatgacct cagccgcagg ctttccgtaa acacagtagt catcattttc acttgcatcc
    11461 tcaagacgat aaagcttagc tggccaatcc cataacataa atccataaaa ccacttttgc
    11521 ccatgaaaga atttaaacat ctcttcataa aacactcttt gttcgtccat atcgatttgc
    11581 cctcgattct tattccaatc gttaggaaca gaaccagatt cgagacggct cggacaccct
    11641 gcttccatga agaagaacgg tttttgccac gactccacaa ttttttttat gcgagattca
    11701 tgatgttccc aagaccctat tgggtaataa ccactagaag acatcacgtc aactgcatcc
    11761 caccaggtca cttcgtcttc ttgatatttg tcacagttat acgtgatgat gccagaatac
    11821 acttggcgga ctttttgaat taaatcccgc cattcttttt ctctcctttc tgtctgtacc
    11881 atttcacagc caatgcaaag catctcacat cctgtatcct ctgctaattt ggcataatgt
    11941 aacatgaaag actcatagct tttaaaccac tgtgaccatg taggttcaca agggacatcc
    12001 ttatcaaaaa aattaatatg cgctcgccac gttccgtctc ttacattaac aataggcttt
    12061 aagatgacgc ttagaccaag acttttcgct ttggcaattg cccaccgtac gttctcatct
    12121 gtgaccattg gtggctctgc aaatgttatg tccggtgaat gagccgtctc ttgtaaacct
    12181 tgaaaagcaa ttgctgtcca gttaattgct aagtttgtca tttcttccat agaacgttct
    12241 gcctcgtttg aacgccaatc ctcgctattt cctatccagc cccacgtcat acctttaata
    12301 tagtccatcg aaaaatgtcc tttctcaagt taagatttac aacgatcgat acgctctatt
    12361 aattcaaagc aacatcggga attatggtaa gggcacttcc aaggctctac tttaggtcta
    12421 tcaagatagg cattgttttg ctcatctaat ttccaatacc attcaccata gtgcttatcg
    12481 accatatgct gtttagtgaa tcgccataac ttatagaccg cttctaaata atcgttactt
    12541 aacgttaatt gataggcatt aaagaagcct atcatccctt ctgcctgcac ccaccatact
    12601 ttttcttcat ccacatcgtc gccttcacgt tcatttatta atcctccact ttcagaaaag
    12661 ccctctatcg ctactttttc tgccatatca atcgatgttc ttcttacttt ttcaagtcgc
    12721 ttttcatctc ctagaacttc tgcagcttca tataacaacc agctgccttc aatatcatgt
    12781 ccataagata caatatttgc cattccatgc cactgtcgat caaaaaatag cgtgaattga
    12841 tcagtcgggc caacgatgtg ttttagcatc acatcgatca gctcagtaat ccgctccttt
    12901 agttgaggac ttttccacac cttaaataga ttcgtatagg cttctaaaat atgaaggtgt
    12961 gtattcatag atttttccgc ctccatatca ctttcactta aacgaggatc ttgctccatc
    13021 ttccactctt tagtaaacgc ttccaaataa cctttatact gcctatcgta tccgtatttc
    13081 tctattaaat aaaagatgtc ttttgctatt tgaagacttt cttcatggca tgttattttg
    13141 aaatactcag ctaacgcata gatagcgaag gattggttgt atatgtgctt tcgtgtttct
    13201 tgaggtttcc ctttatgatc caccatccaa taaacaccac catcttcctt atctaaaaag
    13261 aagtctctta aatagacata agcatgattc gcagtctcta agtagtgacg attaccaaat
    13321 acatgatagg cctttgaaaa ggtccaaaga attcttgagt taagaatgcc ccccttaggt
    13381 gccttctcgt ctactttatt atgagtcgtg agatacccgt aaaatccgcc gtttgtttta
    13441 tccatcacat gtgttaccca ataagggaga atattagcca ctaattcatt tttaagctct
    13501 tgcgacaatt catctaactt tctttgcttc tccaattctt tcacctcctt gcatcaattt
    13561 attatcacct gtaaattact ttatcgttta agttataaaa taatcatata gtgataacgc
    13621 ttacttgtca atctttaatc accttaaaat tggtattatt gaatactaaa gtcggctctc
    13681 atgcattcaa aaagggggac acagctaatg taagtctatg acgacggctc aagtcatgaa
    13741 aaaagctgtt tcaaaagctt agacttttga aacagcatcg tggctttcca attatgaacg
    13801 taaatattgc atgattatat cgtgaagacc cggcaaattt tcatgtgaaa agtctgggta
    13861 gatttctaat tgtttttctg acttaatgcg attaaatgct gcaaactgtg ttgaaggggg
    13921 acaaatggta tccatcaatc cagttgccat gagcgttttt gctttaatcc tttcggctag
    13981 gtgctgaatg tcgatatatc caagagtcgt aaagacctct tcgacacgct tatgctgtgg
    14041 atcaaaacgt cgaaaataat gtttaatatc ctcatacgca tcaatcgcta aatctaattc
    14101 ccacacacgt ttaaaatcac ttaaaaaagg ataaacagcc gctgcttttt ttattcgcgg
    14161 ctccaatgag gcacatacta acgttaaagc accaccttgt gaccagcctg tagccgatac
    14221 attcgctcca tcaatctcgt ccattccaat tactatttgg gctaattggg cacaatcgag
    14281 ataaacatcc cgaaaaaata agttatcttt atcgtcatcc agccctctta tgatatgacc
    14341 ttttaatgta ttccctttaa caccgccaac atcctctgat tctccccctt gtcctctgac
    14401 atccattgta aatacggcat gacctaacgc cgcataagcc aatttactgc tccaatcccc
    14461 agcgttcata ctgtagccgt gaaacatgag cacagctggc acagggtcag acacagagac
    14521 tggcttcaca tatttcacat gaatacgcgc ccctttaaca cccgtaaaat acatgtcata
    14581 gcaagtggca aaaggaacct ggaagtcact tttcttaaaa tggacttgcg gatcgaccgc
    14641 acgcatttca ctaagcccac gttcccaata gtcatcaaaa tcaaccggtt ttggattagt
    14701 ccctttgtat acttttagtt cggataaagg taagtcaatt gtcggcacgt taatccactc
    14761 ctctttattt atattcaaat taccttaagg ataactactt aatagtttac ctgaaaaatg
    14821 aataacctca atccctgtgc gactttttat tcaatttaat aaaacattat ataacaaact
    14881 atattaaatt ttgttattaa gtaaatggta ttaaaataaa cctctttatt gactttatcg
    14941 attacctaat ttacctttaa ggaaacgctt tcttttttgc cattttgaaa gatagattaa
    15001 aaggcttggt cacttgagcc tacttccgct tcttgattat ctgaagaacg gcccgaaagg
    15061 aggagtgtga cttgccactc agataatata aataacccac aaggaggagt aataacatga
    15121 aaaaaaagtt atcacagatt tatcatttaa ttatttgcac acttataata agtgtgggaa
    15181 taatggggat tacaacgtcc ccatcagaag caagttcagg cttttatgtt gatggcaata
    15241 cgttatatga cgcaaacggg caaccatttg tcatgaaagg cattaaccat ggacatgctt
    15301 ggtataaaga caccgcttca acagctattc ctgccattgc agagcaaggc gcgaacacga
    15361 tacgtattgt tttatcagat ggcggtcaat gggaaaaaga cgacattgac accgttcgtg
    15421 aagttattga gcttgcggag caaaataaaa tggtggctgt cgttgaagtt catgatgcca
    15481 cgggccgtga ttcacgcagt gatttagatc gggcagtcga ttattggata gagatgaaag
    15541 atgcacttat cggcaaagag gatactgtca ttattaacat tgcaaacgaa tggtatggca
    15601 gttgggatgg cgccgcttgg gctgatggct acattgatgt cattccgaag cttcgcgatg
    15661 ccggcttaac acacacctta atggttgatg cagcaggatg ggggcaatat ccgcaatcta
    15721 ttcatgatta cggacaagat gtgtttaatg cagatccgtt aaaaaatacg atattctcca
    15781 tccatatgta tgagtatgct ggtggtgatg ctaacactgt tagatcaaat attgatagag
    15841 tcatagatca agaccttgct ctcgtaatag gtgagttcgg tcatagacac actgatggcg
    15901 atgttgatga agatacaatc cttagttatt ctgaagaaac tggcacagga tggctcgctt
    15961 ggtcttggaa aggcaacagt gccgaatggg attatttaga cctttcagaa gattgggctg
    16021 gtaaccattt aactgattgg ggaaatagga ttgtccacgg ggcaaatggc ttgcaggaaa
    16081 cctccaaacc atccaccgta tttacagatg ataacggtgg tgcccctgaa ccgccaacta
    16141 ctactacctt gtatgacttt gaaggaagca cacaagggtg gcatggaagc aacgtgatgg
    16201 gtggcccttg gtccgtaaca gaatggggtg cgtcaggcaa ctactcttta aagggcgatg
    16261 tcaatttaag ctcaaattct tcacatgaac tgtatagtga acaaagtcgt aatctacacg
    16321 gatactctca gctaaatgca accgttcgcc atgccaattg gggaaatccc ggtaatggca
    16381 tgaatgcaag actttacgtg aaaacgggct ctgattatac atggtatagc ggtcctttta
    16441 cacgtatcaa tagctccaac tcaggtacaa cgttatcttt tgatttaaac aacatcgaaa
    16501 atagtcatca tgttagggaa ataggtgtgc aattttcagc tgcagataat agcagcggtc
    16561 aaactgctct atacgttgat aatgttactt taagatag
//
