LOCUS       AF027499                5591 bp    DNA     linear   BCT 25-JUL-2016
DEFINITION  Azotobacter vinelandii mannuronan C-5-epimerase (algG) gene, partial
            cds; and AlgX, alginate lyase (algL), AlgI, and AlgV genes, complete
            cds.
ACCESSION   AF027499
VERSION     AF027499.1
KEYWORDS    .
SOURCE      Azotobacter vinelandii
  ORGANISM  Azotobacter vinelandii
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Azotobacter.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Vazquez,A., Moreno,S., Guzman,J., Alvarado,A. and Espin,G.
  TITLE     Transcriptional organization of the Azotobacter vinelandii
            algGXLVIFA genes: characterization of algF mutants
  JOURNAL   Gene 232 (2), 217-222 (1999)
   PUBMED   10352233
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Vazquez,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-1997) Microbiologia Molecular, Inst. de
            Biotecnologia. UNAM, Av. Universidad s/n Col. Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Azotobacter vinelandii"
                     /mol_type="genomic DNA"
                     /strain="ATCC 9046"
                     /db_xref="ATCC:9046"
                     /db_xref="taxon:354"
     gene            <1..99
                     /gene="algG"
     CDS             <1..99
                     /gene="algG"
                     /note="AlgG"
                     /codon_start=1
                     /transl_table=11
                     /product="mannuronan C-5-epimerase"
                     /protein_id="AAC04565.1"
                     /translation="LEDKQEEILDLLVRRQKAVLIDPVVDPAQAEL"
     gene            120..1571
                     /gene="algX"
     CDS             120..1571
                     /gene="algX"
                     /note="similar to Pseudomonas aeruginosa AlgX"
                     /codon_start=1
                     /transl_table=11
                     /product="AlgX"
                     /protein_id="AAC04566.1"
                     /translation="MKTHNSKWIGPAALAAAIALAAAGVRAEETPTGLPVYRAESCCDL
                     CPAAADPNSYTSNYMKGFVTLVQGNESDWLFRTNEDLRTEFGTTPEGYRQLKALHDAFK
                     SRGVELVIVYQPTRGMVQRNKLLPADYARFDYDKAVRNFRATLKHFEQLGYWVPDLTPL
                     TDEKVEPAFYFRGDHHWTSYGAERSARIVAETVKEIPAFADIPRKEFVTKKMGRMGKRG
                     THHRVAGQLCNTTYAFEHSDQFFTEPKGEGGGDLFGDSSLPQITLVGTSHSGTNYNFAG
                     FLSEYMGAEILNVAFPGSGLEGSMLKYLASDEFQKNPPKILIWEFSPLYDLAEDKFYRQ
                     ALSMLGNACEGEKTLLAGKATLRPGEAGKEVLINGAGRLVEATNSRHQVDIRFSDPSVK
                     KLEGTIWYMTGRREKFQFDKPVTTETNGRFAFNMRDEADWGGLNFFAMEIQPPEGLKEP
                     VEVEVRLCKRHDYHAPANLTARSGN"
     gene            1580..2704
                     /gene="algL"
     CDS             1580..2704
                     /gene="algL"
                     /note="AlgL; similar to Pseudomonas aeruginosa AlgL"
                     /codon_start=1
                     /transl_table=11
                     /product="PL5|PL5_1"
                     /protein_id="AAC04567.1"
                     /translation="MHKTRLALSCLLGSLLLSGAVHAAEALVPPKGYYAPVDIRKGEAP
                     ACPVVPEPFTGELVFRSKYEGSDAARSTLNEEAEKAFRTKTAPITQIERGVSRMVMRYM
                     EKGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPL
                     AAYPEQARRIESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDW
                     AVEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGVDLRGDNDG
                     ALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWLEPYCALYSCSPALRERK
                     AEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKRD"
     gene            2897..4396
                     /gene="algI"
     CDS             2897..4396
                     /gene="algI"
                     /note="similar to Pseudomonas aeruginosa AlgI"
                     /codon_start=1
                     /transl_table=11
                     /product="AlgI"
                     /protein_id="AAC04568.1"
                     /translation="MVFSSNVFLFLFLPLFLGLYYLSPARYRNLLLLTASYVFYAWWRI
                     DFLGLFAAVTVFNYWIGLRIGAAGVRTQSAQRWLTLGVVVDLCVLGYFKYANFGVDSLN
                     AIITSFGVEPFVVTHILLPIGISFYVFESISYIIDVYRGDTPATRNLVDFAAFVAIFPH
                     LIAGPVLRFRDLVDQFNHRTHTLDKFAEGCTRFMQGFIKKVFIADSLAPIADHCFALSD
                     PTTGDAWLGALAYTAQLYFDFSGYSDMAIGLGLMIGFRFMENFNQPYISQSITEFWRRW
                     HISLSTWLRDYLYISLGGNRGTTFQTYRNLILTMLLGGLWHGANWTFIIWGAWHGTWLA
                     IERALRIDAAPKTIRPLRWVFAFLLVMVGWVIFRAENLDVAWRMYAAMFSFGDWTLSDL
                     NKAQLTSLQIATLLLAYVVIAVYGIRQFYAQPLTGAPKAKANDQADAPQGIVHASSGTL
                     AYSQAIDIPALATRVAVLLLFAASVLKLSAQSFSPFLYFQF"
     gene            4409..5575
                     /gene="algV"
     CDS             4409..5575
                     /gene="algV"
                     /note="similar to Pseudomonas aeruginosa AlgJ"
                     /codon_start=1
                     /transl_table=11
                     /product="AlgV"
                     /protein_id="AAC04569.1"
                     /translation="MNRTTNLVYAGTFAGTLLALSLWSLKGAAGFSTADNTPVLNGKLA
                     LAFEKHYDEEFPIKKLGTNLWAALDYTLFGEGRPGVVIGANQWLFSDEEFKPTAAASQN
                     ITDNQALIQGVRETLARNNVQLVMAILPAKARLYPENFGEQQPASLHEQLYQNFRRIVA
                     DAGIQAPDLLGPLQQAKAGGQVFLRTDTHWTPYGAQVVAGQLATTIKPIGVLPESGNVY
                     VTETLPGGPHKGDLTNFLPLDPLFEELLPPPDQLAKHNTRQQEESAPAGDDLFAETQVP
                     VALVGTSYSADERWNFAGALRQALGSDLVNFAEDGRGPLLPMLKFLQSEDFKKSPPRLV
                     IWEFPERYLPMAYDLSEFDADWIAQLKAAGRQDKQLADNTATNQGARH"
ORIGIN
        1 ctcgaggaca aacaagaaga gatcctcgat ctgctggtgc gccgccagaa ggccgtgctg
       61 atcgaccccg tcgtcgatcc cgcccaggcc gagctgtaga accgatgaga ctgcgcaaca
      121 tgaagaccca caacagcaaa tggattggac ccgccgcgct ggccgcggcg atcgccctgg
      181 ccgccgccgg ggtgcgtgcc gaagaaaccc ctaccgggct gcccgtctac cgcgccgagt
      241 cctgctgcga cctgtgcccg gccgccgcgg accccaacag ctacaccagc aactacatga
      301 agggcttcgt caccctggtg cagggcaacg agagcgactg gctgttccgt accaacgagg
      361 acctgcgcac cgaattcggt accactccag aaggttaccg tcagctcaag gcattgcacg
      421 acgccttcaa gagccgcggc gtcgagttgg tgatcgtcta ccagccgacc cgcggcatgg
      481 tgcaacgcaa caaactgttg cccgccgact acgcccgctt cgactacgac aaggcggtga
      541 ggaacttccg ggcgaccctg aagcatttcg agcaactcgg ctactgggtg cccgacctga
      601 ctccgctgac cgacgaaaag gtcgagccgg ccttctactt ccgcggcgac caccactgga
      661 cctcctacgg cgccgaacgc agtgcgcgga tcgtcgccga gaccgtgaag gagatccccg
      721 ccttcgcgga catcccgcgc aaggagttcg tcaccaagaa aatgggccgc atgggcaagc
      781 gcggcaccca ccaccgggtc gccggccagt tgtgcaacac cacctacgcc ttcgagcaca
      841 gcgaccagtt cttcaccgaa cccaagggtg aaggcggcgg cgacctgttc ggcgacagca
      901 gcctgccgca gatcaccctg gtcggtacca gccacagcgg caccaactac aacttcgccg
      961 gcttcctctc cgaatacatg ggtgccgaaa tcctcaacgt cgccttcccc ggcagcggcc
     1021 tagaaggctc gatgctcaaa tacctggcca gcgacgaatt ccagaagaat ccgccgaaga
     1081 tcctcatctg ggaattctcg ccgctctacg acctggccga ggacaagttc taccgccagg
     1141 cgctgtccat gctcggcaat gcctgcgaag gcgaaaaaac cctgctcgcc ggcaaagcca
     1201 ccctgcgccc cggcgaagcc ggcaaggaag tgctgatcaa cggcgccggc cgactggtcg
     1261 aggccaccaa ctcccgccat caggtggaca tccgcttcag cgacccctcg gtgaaaaagc
     1321 tggaaggcac catctggtac atgaccggcc gccgcgagaa gttccagttc gacaagccgg
     1381 tcaccaccga aaccaacggc cgtttcgcct tcaacatgcg cgacgaggcc gactggggcg
     1441 gactcaactt cttcgccatg gaaatccagc cgccggaggg gctcaaggag cccgtcgagg
     1501 tcgaggtgcg cctctgcaag cgccacgact atcacgctcc ggccaacctg accgcccgta
     1561 gcggcaactg aggcctacca tgcacaagac cagactcgcc ctctcctgcc tgctcggcag
     1621 cctgctgttg agcggcgccg tccacgccgc cgaagccctg gttccgccca agggctacta
     1681 cgcgccggtg gatatccgca aaggcgaggc gccggcctgt ccggtggtgc ccgaaccctt
     1741 caccggggaa ctggtctttc gcagcaagta cgaaggctcg gacgcggccc ggtcgaccct
     1801 caacgaggaa gcggaaaagg ccttccgcac caagaccgcg ccgatcaccc agatcgagcg
     1861 cggcgtcagc cggatggtca tgcgctacat ggagaaaggc cgcgccggcg acctggaatg
     1921 caccctggcc tggctggacg cctgggccga agacggcgcc ctgctcacca ccgagtacaa
     1981 ccacaccggc aaatccatgc gcaagtgggc cctcggcagc ctggccggcg cctacctgcg
     2041 cctgaaattc tccagctcgc agccgctggc ggcctatccc gaacaggcac ggcgcatcga
     2101 gagctggttc gccaaggtgg gcgaccaggt gatcaaggac tggagcgacc tgccgctcaa
     2161 gcggatcaac aaccactcct actgggcggc ctgggcggtc atggccgccg gcgtggcgac
     2221 caaccgccgg ccgctgttcg actgggcggt cgagcagttc cacatcgccg ccgggcaggt
     2281 cgactcgaat ggcttcctgc ccaacgaact caagcgccgc caacgtgcgc tggcctacca
     2341 caactacagc ttgccgcccc tgatgatggt cgccgccttc gccctggcca acggtgtcga
     2401 cctgcgcggc gacaacgatg gcgccctggg ccgcctggcc ggcaacgtgc tggccggcgt
     2461 cgagaagccc gaaccgttcg ccgaacgggc cggcgacgag gatcaggaca tggaggatct
     2521 ggagaccgac gccaagttct cctggctgga accctactgc gcgctctact cctgctcccc
     2581 cgccctgcgg gaaaggaagg ccgagatggg cccgttcaag aatttccgcc tgggtggcga
     2641 cgtgacccgg atcttcgatc cggccgagaa gtcgcccaga tcgaccgtcg gcaagcgcga
     2701 ctagcgcggc cccgacgtcg ccccagcagc cggcagtcag tccgtcgccg gccgccacgc
     2761 ccttccgggc caggcggaat gccccgaaat gcgcaacaga cgcagccggg aacggacctc
     2821 ttcggggacc ccccggggga gaggccgggc tcagagaaga gcctgaaaca ttggtgctag
     2881 aagcgagaca cgggtaatgg tgttttcatc caacgtcttc ctgtttttat tcctgccgct
     2941 gttcctcggg ttgtactacc tgagcccggc gcgctaccgc aacctgttgc tgctgaccgc
     3001 cagctatgtc ttctatgcct ggtggcgcat cgacttcctt ggactgtttg ccgccgtcac
     3061 cgtcttcaac tactggatcg gcctacgcat cggcgccgcc ggtgtgcgca cccagtccgc
     3121 acaacgctgg ctgacgctcg gcgtggtggt cgacctctgt gtgctcggct acttcaagta
     3181 cgcgaacttc ggcgtggaca gtctgaacgc gatcatcacc tcgttcggcg tggaaccctt
     3241 cgtggtcacc cacatcctgc tgccgatcgg catctccttc tacgtcttcg aatccatcag
     3301 ttacatcata gacgtttatc gcggagatac cccggcgacc cgcaatctgg tcgacttcgc
     3361 ggccttcgtg gcgatcttcc cgcacctgat cgccggtccg gtgctgcgct tccgcgacct
     3421 ggtcgaccag ttcaaccatc gcacccacac gctggacaag ttcgccgaag gctgcacccg
     3481 cttcatgcag ggcttcatca agaaggtgtt catcgccgac agcctggcgc cgatcgccga
     3541 tcactgcttc gccctcagcg atccgaccac cggcgatgcc tggctcggcg ccctggccta
     3601 caccgcccag ctgtacttcg acttctccgg ctacagcgac atggccatcg gcctcggcct
     3661 gatgatcggc ttccgcttca tggagaactt caaccaaccc tacatcagcc agtcgatcac
     3721 cgaattctgg cgccgctggc acatcagcct gtccacctgg ctgcgcgact acctgtacat
     3781 cagcctgggc ggcaaccgcg gcaccacctt ccagacctac cgcaacctga tcctcaccat
     3841 gctgctcggc ggcctgtggc acggcgccaa ctggaccttc atcatctggg gcgcctggca
     3901 cggcacctgg ctggcgatcg agcgcgccct gcgcatcgac gccgcgccga agaccattcg
     3961 cccgctgcgc tgggtcttcg ccttcctgct ggtgatggtc ggctgggtga tcttccgcgc
     4021 cgagaacctc gacgtcgcct ggcgcatgta cgccgccatg ttcagcttcg gcgactggac
     4081 cctgtccgac ctgaacaagg cacaactgac cagcctgcag atcgccaccc tgctgctcgc
     4141 ctacgtggtg atcgcggtgt acggcattcg ccagttctac gcccaaccgc tcaccggcgc
     4201 gcccaaggcc aaggcgaacg atcaggccga cgcgccccag ggcatcgtcc atgcgtcgag
     4261 cggcaccctg gcctattcgc aggccatcga tataccggca ctggcgaccc gcgtcgcggt
     4321 gctcctgctg ttcgccgcct ctgtgctcaa gctttcggcg cagagtttct cgcctttcct
     4381 ctacttccag ttttgaagag ggaccgccat gaatagaaca accaacctgg tctacgccgg
     4441 taccttcgcc ggcaccctgc tggcgctcag cctgtggtcg ctgaaaggcg ccgccggctt
     4501 ctccaccgcc gataacaccc cggtgctcaa cggcaagctg gccctggcct tcgagaaaca
     4561 ctacgacgag gaattcccga tcaagaagct cggcaccaac ctctgggcgg cgctggacta
     4621 cacgctgttc ggcgaaggcc gtcccggcgt ggtgatcgga gccaaccagt ggctgttcag
     4681 cgacgaggag ttcaagccca ccgccgccgc ctcgcagaac atcacggaca accaggcgct
     4741 gatccagggc gtgcgtgaaa ccctggcacg gaacaacgtg caactggtga tggcgatcct
     4801 gccggccaag gcacgcctgt acccggagaa cttcggcgag cagcagcccg cgtccctgca
     4861 cgagcagctc taccagaact tccgccggat cgtggccgat gccggcatcc aggcacccga
     4921 cctgctcggc ccgctgcagc aggccaaggc cgggggccag gtgttcctgc gcaccgacac
     4981 ccactggacg ccctacggcg cccaggtggt cgccggccag ttggccacga cgatcaagcc
     5041 gatcggcgtg ctcccggaaa gcggcaacgt ctacgtcacc gaaaccctgc cgggcgggcc
     5101 gcacaagggc gacctgacca acttcctgcc gctcgacccg ctgttcgagg aactgctgcc
     5161 gccgccggat caactggcca agcacaacac ccgccagcag gaggaaagcg cccccgccgg
     5221 ggacgacctg ttcgccgaaa cccaggtgcc ggtggccctg gtcggcacca gctacagcgc
     5281 cgacgaacgc tggaacttcg ccggcgcgct gcgccaggcg ctcggcagcg acctggtgaa
     5341 cttcgccgag gacggccgcg ggccgctcct gccgatgctc aagttcctgc agagcgagga
     5401 cttcaagaaa tccccgccgc gcctggtgat ctgggaattc cccgagcgtt acctgccgat
     5461 ggcctatgac ctgagcgagt tcgacgccga ctggatcgcc cagctcaagg ccgccggccg
     5521 ccaggacaag cagttggccg acaacaccgc taccaaccag ggcgcacgcc actgaacgcc
     5581 cttccatcga t
//
