LOCUS       Z49992                  6863 bp    DNA     linear   BCT 18-APR-2005
DEFINITION  B.subtilis celA, celB, celC, celD and ywaA genes.
ACCESSION   Z49992
VERSION     Z49992.1
KEYWORDS    6-phospho-beta-glucosidase; cel operon regulator; celA gene; celB
            gene; celC gene; celD gene; cellobiose phosphotransferase.
SOURCE      Bacillus subtilis subsp. subtilis str. 168
  ORGANISM  Bacillus subtilis subsp. subtilis str. 168
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Tobisch,S., Glaser,P., Kruger,S. and Hecker,M.
  TITLE     Identification and characterization of a new beta-glucoside
            utilization system in Bacillus subtilis
  JOURNAL   J. Bacteriol. 179 (2), 496-506 (1997)
   PUBMED   8990303
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Glaser,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-1995) Philippe Glaser, Regulation de l'Expression
            Genetique, Institut, Pasteur, 28 Rue du Dr Roux, Paris, Paris,
            75724, France
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Bacillus subtilis subsp. subtilis str. 168"
                     /mol_type="genomic DNA"
                     /strain="168"
                     /sub_species="subtilis"
                     /type_material="type strain of Bacillus subtilis"
                     /db_xref="taxon:224308"
     regulatory      10..40
                     /regulatory_class="terminator"
     gene            173..2112
                     /gene="celR"
     regulatory      173..179
                     /regulatory_class="ribosome_binding_site"
                     /gene="celR"
     CDS             187..2112
                     /gene="celR"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_11,DBD-Pfam|Mga,DBD-SUPERFAMILY|0039
                     384,DBD-SUPERFAMILY|0039384"
                     /protein_id="CAA90284.1"
                     /db_xref="GOA:P46321"
                     /db_xref="InterPro:IPR002178"
                     /db_xref="InterPro:IPR003501"
                     /db_xref="InterPro:IPR007737"
                     /db_xref="InterPro:IPR011608"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR013011"
                     /db_xref="InterPro:IPR013196"
                     /db_xref="InterPro:IPR016152"
                     /db_xref="UniProtKB/Swiss-Prot:P46321"
                     /translation="MLHGRLRDILRLLMAAEAPVTSSFFAAQLNVTTRTVRNDIKELQG
                     VLSGHGAFVQSVRGSGYKLRIDDEQVFRTLLQDEFQQKKGLPVLPEERMAYLMKRLLLA
                     DHYLKLDELAEELFISKSTLQTDLKEVKKRLLPYRIVMETRPNYGFKLRGDEVQMRYCM
                     AEYIVDERETEIDVLNEKADILPKEEIEIIRSAILKKMKNDRIPLSNMGLNNLIIHIAI
                     ACKRIRTENYVSLFPKDMDHILHQKEYQAAEAIVKELESKLSVTFPKDETAYITMHLLG
                     TKRMTQSQCGEDTFSIEEETDQLTLAMIKAVDRELKLGILHDKELKIGLALHMKPAISR
                     NRYGMNLRNPMLAAIKEHYPLAFEAGIIAGIVIKEQTGIEIHENEIGYLALHFGAAIER
                     KKTESPPKRCIIVCASGAGSAQLLREKLRSHFGKRLDILGTAEYYSLDQMSYESIDFVI
                     STIPIKKELPVPVLKVNTILGGTDFTKIESILSDEKEKANRYLKKELVFFQEDLRSKEE
                     VIQFLGQKVVECGFADEEIIDSIFEREDMSPTCFGNLVAIPHPLVPQTKTTFWAVCTLK
                     KPIDWESQRVQFVCLLCVEKENKADLQSMYKLLGSILDDPAAMNQLIKCRSYQELSDVF
                     DQKMLS"
     stem_loop       2129..2150
     stem_loop       2161..2180
     gene            order(2226..2232,2240..2548)
                     /gene="celA"
     regulatory      2226..2232
                     /regulatory_class="ribosome_binding_site"
                     /gene="celA"
     CDS             2240..2548
                     /gene="celA"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46318|4.A.3.2.2"
                     /protein_id="CAA90285.1"
                     /db_xref="GOA:P46318"
                     /db_xref="InterPro:IPR003501"
                     /db_xref="InterPro:IPR013012"
                     /db_xref="InterPro:IPR014350"
                     /db_xref="UniProtKB/Swiss-Prot:P46318"
                     /translation="MNILLVCAAGMSTSLLVSKMEKSAQEQGKDYTIWAVSGDSVQNHI
                     DKADVLLLGPQVRYMLPQLKKLGESKGVPVDVINTVHYGTCNGAEVLKSAEQLGHVS"
     gene            order(2548..2555,2564..3922)
                     /gene="celB"
     regulatory      2548..2555
                     /regulatory_class="ribosome_binding_site"
                     /gene="celB"
     CDS             2564..3922
                     /gene="celB"
                     /note="gtg start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46317|4.A.3.2.2"
                     /protein_id="CAA90286.1"
                     /db_xref="GOA:P46317"
                     /db_xref="InterPro:IPR003352"
                     /db_xref="InterPro:IPR004501"
                     /db_xref="InterPro:IPR004796"
                     /db_xref="UniProtKB/Swiss-Prot:P46317"
                     /translation="MNKVNQILEEKVMPIAGRIAGQRHLQALRDGIILTMPLIIIGSFF
                     LIIGNLPIPGYAEFMAKTFGSSWSEKLAYPVDATFEIMGLVAAFGIAYRLAEKYGVDAL
                     SAGAISLAAFLLATPYQVPFMPDGATKEIMVGGGIPLSLMGSKGLFVAMIIAMVSTEIY
                     RLIIQRNLVFKMPDGVPPAVSKSFVALIPGFAVIFLIWAARLIVEATPFESLHNIVSVL
                     LGTPLSILGGSLGGSLVAEAVKMLLWACGLHGANIVGGVMAPIWYGAMDANRIAFQAGE
                     ELPKIFTQQFFDIWVNIGGSGATLALVVTMFLRARSKQMKQLGKLAVGPAIFNINEPII
                     FGMPIVMNPMLLLPFIITPLVTVTLTYIGMSTGLVAKPAGIAVPWTMPPIFSGYLATGG
                     KVSGAVMQAINIAVSFVVYYPFFRMWDKQKLKEENDLELVQTPAATDDKEAAL"
     gene            order(3926..3934,3941..4273)
                     /gene="celC"
     regulatory      3926..3934
                     /regulatory_class="ribosome_binding_site"
                     /gene="celC"
     CDS             3941..4273
                     /gene="celC"
                     /note="gtg start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P46319|4.A.3.2.2"
                     /protein_id="CAA90287.1"
                     /db_xref="GOA:P46319"
                     /db_xref="InterPro:IPR003188"
                     /db_xref="UniProtKB/Swiss-Prot:P46319"
                     /translation="MNEEMEQIIFQIILHGGNGRSSAMEAIAAAKSGDAEEARKKLQDA
                     AEELSKAHHYQTELIQNEAGGEKTEMTLLMVHAQDHLMNAMTVKDMAAEIIELYEKITE
                     QRGASI"
     gene            4255..4262
                     /gene="celD"
     regulatory      4255..4262
                     /regulatory_class="ribosome_binding_site"
                     /gene="celD"
     gene            4270..5598
                     /gene="celD"
     CDS             4270..5598
                     /gene="celD"
                     /codon_start=1
                     /transl_table=11
                     /product="GH4"
                     /protein_id="CAA90288.1"
                     /db_xref="GOA:P46320"
                     /db_xref="InterPro:IPR001088"
                     /db_xref="InterPro:IPR015955"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR019802"
                     /db_xref="InterPro:IPR022616"
                     /db_xref="UniProtKB/Swiss-Prot:P46320"
                     /translation="MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGE
                     EKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPL
                     KYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYS
                     NLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIE
                     AMGDPKNAMTMKNISGAEWEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRA
                     EVVQKVEKELFELYKDPNLAIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTIN
                     NGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDY
                     QTALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA"
     regulatory      5597..5630
                     /regulatory_class="terminator"
     gene            complement(5632..6736)
                     /gene="ywaA"
     CDS             complement(5632..6723)
                     /gene="ywaA"
                     /codon_start=1
                     /transl_table=11
                     /product="Unknown, highly similar to yeast TWT1 protein and
                     to Mouse ECA39 protein, similar to amino acid
                     aminotransferases"
                     /protein_id="CAA90289.1"
                     /db_xref="GOA:P39576"
                     /db_xref="InterPro:IPR001544"
                     /db_xref="InterPro:IPR005786"
                     /db_xref="InterPro:IPR018300"
                     /db_xref="UniProtKB/Swiss-Prot:P39576"
                     /translation="MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWY
                     DPRIIPYQPLSMDPTAMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLC
                     IPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIIL
                     SPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLW
                     LDGIEKKYIEEVGSMNIFFKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKI
                     AIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLSINNGETGEIAKKLYDTITG
                     IQKGAVADEFGWTTEVAALTESK"
     regulatory      complement(6730..6736)
                     /regulatory_class="ribosome_binding_site"
                     /gene="ywaA"
ORIGIN
        1 ggatcctcta gaacagccgg ctgatcccgg ctgttttttt ataggtcagt tttccctcta
       61 gtcctccgga tgttcattga cttgccattc tctccattat agaatttata tacatttcgc
      121 atcccttcat catcctttct gcctattcac tgaatccctg catctactag ttaggaggtt
      181 tttctcatgc ttcatgggcg actacgtgat attctgcgct tgctgatggc ggctgaagcc
      241 ccggtgacaa gttcgttttt tgccgcgcaa ttaaacgtaa cgacgcggac agtccgcaat
      301 gatataaagg aacttcaagg tgtgctaagc ggccacggtg cctttgtaca atctgtaaga
      361 ggctccggct acaagctccg cattgatgac gagcaagtat tcagaacact gctgcaagat
      421 gaatttcagc aaaaaaaggg cctgccggta ttgccggagg agagaatggc ctatcttatg
      481 aaacgtctgc tgctggcgga ccattatctc aagctcgatg aacttgctga agaactattt
      541 atctcaaaat ctacactcca aacggattta aaggaagtaa agaaacgtct gctgccttac
      601 cgcatcgtca tggaaacacg tccgaattac ggcttcaagc tgcgcgggga cgaggtgcag
      661 atgaggtact gcatggcgga gtacattgtt gatgaaagag aaacggagat agatgtttta
      721 aatgaaaagg ctgatatttt gccaaaagaa gaaattgaga tcatccgttc cgccatcttg
      781 aaaaaaatga aaaacgaccg catccctctg tccaacatgg gactgaataa cctcattatc
      841 catatcgcta tcgcctgtaa acggatcaga acggaaaact atgtttctct tttcccgaaa
      901 gatatggatc atatcctgca tcaaaaggag taccaggctg cagaagccat cgtaaaagag
      961 ctggaatcaa agctttccgt tacgtttccg aaagacgaga cggcctacat caccatgcat
     1021 ctccttggca caaaacgaat gacacaatca caatgcggag aggacacatt ttcaatagaa
     1081 gaggaaaccg atcagctgac acttgccatg atcaaagctg ttgatcgtga actgaagctt
     1141 ggcatcctgc atgacaaaga gctgaaaatc ggcttggcac tgcatatgaa gccggcgatc
     1201 agccgcaacc gatacggcat gaatcttcga aatccgatgc tggcagcgat caaggaacat
     1261 tatccactcg cttttgaagc cgggatcatt gccggaatcg tgataaagga acaaacgggt
     1321 attgagattc atgaaaacga aattggatat ttggctctcc actttggcgc cgcgattgaa
     1381 cgaaaaaaaa cagaaagccc gccgaaacgc tgtatcattg tatgcgcatc gggagccgga
     1441 agcgcccagc ttctgcgcga aaaactgcgt tcccatttcg gcaagcggct cgatattctt
     1501 ggaacggctg agtattatag tcttgatcaa atgtcctacg aatcaattga ttttgtcatc
     1561 agcaccattc cgattaaaaa agagctccct gtaccagtac tgaaggttaa caccatctta
     1621 ggcggaactg actttacaaa aatagaatct attctgagtg atgaaaagga aaaagccaat
     1681 cgctacctaa aaaaagaact cgtttttttt caggaggatc tgcgctcaaa agaagaagtg
     1741 attcaatttt tagggcagaa agtggtcgaa tgcggctttg ccgatgaaga aatcatagat
     1801 tccatttttg aaagagaaga catgtcgcca acgtgttttg gcaatcttgt tgccatcccg
     1861 cacccgcttg ttccgcagac gaaaacaacg ttttgggccg tttgtacgct caaaaaaccg
     1921 atcgattggg agagtcagcg agttcaattt gtctgtctgc tttgtgtcga aaaggagaac
     1981 aaagcggatt tacaaagcat gtataaactg cttggcagca ttttggatga cccggcggcg
     2041 atgaaccagc tgatcaaatg ccgttcatat caagagcttt cggatgtgtt tgaccaaaaa
     2101 atgctgagct gacagcctgt atataccttt tttccgttgc tgcggaaaaa ccctgagtgt
     2161 ttatgaaagc gatttcataa tatgatgata gcaacagcag gacattacaa aattgatcaa
     2221 actctagggg gtcttttaca tgaatatatt actcgtttgt gcagcaggca tgtctactag
     2281 cttactggtg agcaagatgg aaaaaagtgc gcaggaacag ggaaaagact ataccatttg
     2341 ggcagtttca ggcgattctg tacaaaatca tatcgataaa gcggatgtat tgcttctcgg
     2401 tccgcaagtc cgttacatgc ttccgcagct caaaaaatta ggagagtcaa agggggttcc
     2461 ggtagacgtc attaataccg tccattatgg aacgtgcaat ggggcggaag tgctgaaatc
     2521 agctgaacag cttggacatg tgtcatagag aggggcgggc tatgtgaata aagtcaatca
     2581 gatcttagaa gaaaaggtca tgcctatagc gggaagaatt gcgggacagc gtcatttgca
     2641 ggcgctgcgt gatggtatca tcctcacgat gcccctgatt atcatcggtt cctttttcct
     2701 catcattggc aacctcccga tcccgggata cgctgagttt atggcaaaaa cgtttggcag
     2761 ctcctggtct gaaaaactgg cctacccagt cgacgcgacc tttgaaatta tgggtcttgt
     2821 cgccgcgttc ggcattgcct accggctcgc cgagaaatac ggtgttgatg ccttgtcggc
     2881 cggagccatt tcccttgcgg catttttatt agcaacacct tatcaagtgc cgtttatgcc
     2941 ggatggcgcg acgaaagaaa tcatggttgg cggcgggatt ccgctttcgc tcatgggaag
     3001 caaaggctta tttgtcgcca tgattatcgc aatggtgtcc acagagattt accgccttat
     3061 tatccagcgc aatttagtct tcaaaatgcc ggacggtgtc ccgccggcgg taagcaagtc
     3121 atttgtcgcg ttaatccccg gctttgccgt catcttccta atttgggcag cccggctgat
     3181 tgttgaagcg acaccgtttg aaagccttca taatattgta agcgttttat tggggactcc
     3241 gctttccatt ctgggaggaa gccttggagg aagccttgtc gccgaagccg tcaaaatgct
     3301 tttatgggcc tgcggccttc acggtgcaaa cattgttggc ggcgtcatgg caccgatttg
     3361 gtacggcgca atggatgcca accggatcgc attccaagcc ggtgaggagc ttcctaagat
     3421 tttcacccag caatttttcg atatctgggt caatattggc ggaagcggcg ccacattggc
     3481 actggttgtc acgatgttcc tgcgcgcccg aagcaaacaa atgaagcagc tcgggaagct
     3541 tgcggtcggc ccggccatct tcaatattaa cgaaccgatt atattcggaa tgccgatcgt
     3601 catgaaccca atgctcttac ttccgtttat catcacgccg cttgtcacag tgacactcac
     3661 gtacatcgga atgagcaccg gccttgtcgc caaaccggcg ggtatcgcag tgccgtggac
     3721 catgccgccg atcttttcag gctacttggc gacaggagga aaagtctcag gcgcggtcat
     3781 gcaagccatt aatattgccg tgtcctttgt tgtctactat ccattcttca gaatgtggga
     3841 taagcagaag ctgaaagaag aaaacgatct ggagctggtc caaacgcctg cagcaacaga
     3901 tgacaaagaa gcggctctgt agagaaagga agtgaatcgc gtgaatgagg aaatggaaca
     3961 aatcattttt caaatcatcc ttcacggagg aaacggtagg agctccgcaa tggaggccat
     4021 tgccgctgcg aaaagcggtg acgcagaaga agcgcggaaa aagcttcaag acgctgctga
     4081 agaattgtca aaagcccatc attatcagac agagctgatc caaaatgaag cgggcggaga
     4141 aaagacggaa atgacgcttc tgatggttca tgcacaggat catttaatga atgcaatgac
     4201 agtgaaggat atggctgcag agatcattga gttatatgaa aaaatcaccg agcaaagggg
     4261 agcaagcata tgacaaaagg attgaagatt gtaacgattg gcggaggttc aagctatacg
     4321 cccgagctag tggaggggtt cattaaacgg tatgatgagc tgcctgtccg tgaattatgg
     4381 cttgtcgata tccccgaggg cgaagaaaag ctgaatattg tcggcacact cgccaaacgg
     4441 atggttgaaa aagctggcgt cccgattgac attcatctta cgctcgaccg gagaaaggcg
     4501 ctgaaagacg cagactttgt cacgacccaa ttccgcgtcg gacttttgca ggcaagagca
     4561 aaggatgagc gcattccgct aaaatacgga gtgataggcc aggaaacaaa cggcccggga
     4621 ggtctgttta aaggattgcg cacaattccg gtcatccttg agatcgcaaa ggacatagaa
     4681 gagctttgcc cgaacgcttg gcttgttaac ttcacaaacc ctgccggcat ggtaacagaa
     4741 gccttgcttc gctactccaa cctgaagaaa gtcgtcggtc tttgcaatgt tccaatcggc
     4801 atcaaaatgg gcgtagccaa agcgcttgat gtagatgtgg accgcgtaga agtccaattt
     4861 gccggactga accatatggt attcgggctg gatgttttct tggacggcgt aagtgtgaag
     4921 gagcaagtca tcgaggcgat gggcgacccg aaaaacgcga tgacgatgaa aaatatctca
     4981 ggcgccgaat gggaaccgga tttcttaaaa gcgctcaatg tcatcccttg cggctatcac
     5041 cgttactatt ttaaaacaaa agagatgctg gagcacgaac tggaagcatc acaaacggag
     5101 ggcacccgcg ctgaagtcgt acagaaggtc gaaaaagagc tgttcgagct ttataaagat
     5161 ccaaaccttg cgatcaaacc gccgcagctg gaaaaacggg gcggagctta ctacagtgat
     5221 gcagcatgta acttaatcag ctccatttat aatgataaac atgacattca gccggtgaac
     5281 acgatcaata atggtgcaat agcgagtatt ccagacgatt ccgcagttga agtgaactgt
     5341 gtgatgacga aaaccggccc taagccaatt gctgtcggcg acttgccggt gtctgtccgc
     5401 ggccttgtcc agcaaatcaa atcctttgaa cgtgtcgcgg cagaagcggc agttacaggt
     5461 gactatcaaa ctgcgctcct tgccatgacg attaacccgc ttgtcccgtc cgatactgtg
     5521 gccaaacaga ttttagacga aatgctagag gcgcacaaag cgtatctgcc gcagtttttc
     5581 aacaaaattg aagcgtaaaa aaagccggcc tgtaatgggc cggctttttt cttacttgct
     5641 ttcagtcagc gctgcgactt cggtcgtcca tccgaattcg tctgcgacag cgcctttttg
     5701 aatgcctgta atcgtgtcat atagtttttt tgcgatttct cctgtttcac cgttgttgat
     5761 cgaaagtgtt tcatcctgcc agatcagctc gccgactggg gaaataacag ctgctgtacc
     5821 tgttccgaag gcttcttcca ggatgccgtc tttatgggct tggatgacct catcgatcgc
     5881 aatttttcgt tctgaaactt gaaggcccca atgcttaagc aaggcgatga ctgaattgcg
     5941 cgtaatgcct tccaggatgc tcccgttcag catcggtgtt acgatttcac cgttgatttt
     6001 gaagaagatg ttcatgcttc cgacttcttc gatgtatttc ttctcaatgc cgtccagcca
     6061 gagtacttga gaaaatcctt tctcttcggc tacctgctgc gcttttaagc ttgaagcata
     6121 gtttcctgcg gttttggcat ttcctgttcc gccttttacc gcacggacaa attcactttc
     6181 aacagcgatt ttgaccggct taatgccttc tttgtaataa gagccgaccg gagaaagaat
     6241 gatcaagagc ttatacgtat gagatgccgc aacaccaagg aaaggctcgg ttgcgatgat
     6301 gaacggacgg atgtaaaggg acgtgccctc cgcatttgga atccagtctt tatcaattgc
     6361 gacaagctgc tttaagcctt caagaacctg ttcttcatca atttgcggga tgcagaggcg
     6421 gtcgtttgat tgattcaggc gttccatatt tttttccggt ctgaaaagca gaacatggtc
     6481 atcctctgac acgtaagcct ttaacccttc aaacacggtt tggccgtagt gatagaccat
     6541 tgcagttgga tccattgata agggttgata aggaatgatt cttggatcgt accaaccttt
     6601 atctgcggca tagtccatta caaacatgtg gtctgtaaac actcttccga acgaaagctg
     6661 atttgggtct ggtttcggtt tttttgttga tgtcaattca acgcgaattg tttgtttagt
     6721 catggtcaat ctccctgctg ttaaattgtt tatattatca aacttatttg aaaataatta
     6781 tattctattg tataccttca acaaataatc gcaataggtt tgctcaaatt tgtaagaaat
     6841 tcatatgaaa aaagcaggag aaa
//
