LOCUS       NC_011898              14790 bp    DNA     linear   CON 04-FEB-2020
DEFINITION  Ruminiclostridium cellulolyticum H10, complete sequence.
ACCESSION   NC_011898
VERSION     NC_011898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00623037
            Assembly: GCF_000022065.1
KEYWORDS    RefSeq.
SOURCE      Ruminiclostridium cellulolyticum H10
  ORGANISM  Ruminiclostridium cellulolyticum H10
            Bacteria; Firmicutes; Clostridia; Clostridiales;
            Hungateiclostridiaceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Saunders,E.,
            Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Clostridium cellulolyticum H10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14790)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Saunders,E.,
            Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/04/2020 07:32:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 3,537
            CDSs (total)                      :: 3,447
            Genes (coding)                    :: 3,334
            CDSs (with protein)               :: 3,334
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 37 of 113
            Pseudo Genes (incomplete)         :: 81 of 113
            Pseudo Genes (internal stop)      :: 17 of 113
            Pseudo Genes (multiple problems)  :: 20 of 113
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            CP001348.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4002584
            Source DNA and bacteria available from Jizhong Zhou
            (jzhou@rccc.ou.edu)
            Contacts: Jizhong Zhou (jzhou@rccc.ou.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Ruminiclostridium cellulolyticum H10"
                     /mol_type="genomic DNA"
                     /strain="H10"
                     /culture_collection="ATCC:35319"
                     /type_material="type strain of Ruminiclostridium
                     cellulolyticum"
                     /db_xref="taxon:394503"
     gene            complement(1..456)
                     /gene="tnpA"
                     /locus_tag="CCEL_RS10665"
                     /old_locus_tag="Ccel_2108"
     CDS             complement(1..456)
                     /gene="tnpA"
                     /locus_tag="CCEL_RS10665"
                     /old_locus_tag="Ccel_2108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="WP_015926613.1"
                     /translation="MANKENSLAHTKWMCKYHIVFTPKYRRKVIYNQYKQSIREILIRL
                     CKYKGVEILEGHLMPDHIHMLVSIPPKISVSTFMGYLKGKSALMIFDKHANLKYKFGNR
                     HFWAEGFYVSTVGLNEATIRKYIQEQEKHDIMVDKLSVKEYEDPFKG"
     gene            complement(718..3153)
                     /locus_tag="CCEL_RS10670"
                     /old_locus_tag="Ccel_2109"
     CDS             complement(718..3153)
                     /locus_tag="CCEL_RS10670"
                     /old_locus_tag="Ccel_2109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH94"
                     /protein_id="WP_015925554.1"
                     /translation="MKYGFFDDTNREYVITTPKTPYPWINYLGTQEFFSLISNTAGGYC
                     FYKDARLRRITRYRYNNVPIDMGGRYFYINDNGVLWSPGWSPVKADLDKYECRHGLGYT
                     KITGSKNGISTEVLYFVPLNFNGEIHRVRVKNTTSDNKSVKLFSCIEFCLWNAYDDMTN
                     YQRNLSTGEVEVENSVIYHKTEYKERRNHFSFYSVNADLTGFDTDRDEFIGLYNGFDAP
                     QVPVSGEPKNTIAHGWSPMASHCITVDLKAGEEKELDFILGYVENDVNEKWESKGVINK
                     KKAYKMIEEKGNPAGVQTAFDELQNYWSQLFTQYKLEHKDEKLSRMVNIWNQYQCMVTF
                     NMSRSASYFETGIGRGMGFRDSNQDILGFVHQIPDRARERILDIAATQLENGGAYHQYQ
                     PLTKKGNNEIGGNFNDDPVWLIASVAAYIKETGDMDILKENVPFDNDDTKAASLFEHLR
                     RSFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSETPDESFQTTTSKDGKVAESVLIAGM
                     FVYYGPEYVKLCELNGLEDEAAKAQVEIDKMIKTVKEYGWDGEWFIRAYDDNSEKIGSN
                     ENEEGKIFIESQGFCSMAEIGLEDGYVEKALDSARKYLDTPYGLVLQNPAFTKYYVNMG
                     EISTYPAGYKENAGIFCHNNPWIIAGETVLGRGDRAFEYYSKIAPAYTEEISDIHKTEP
                     YVYSQMIAGKDAKRPGEAKNSWLTGTAAWNFVVISQNILGIKPDYSGLKIDPCIPASWD
                     GYKITRKFRNAVFEIVISNPEHVSKGVKKVVVDGKEIGGNIIPIFNDEKTHQVEVIMG"
     gene            complement(3888..4715)
                     /locus_tag="CCEL_RS10675"
                     /old_locus_tag="Ccel_2110"
     CDS             complement(3888..4715)
                     /locus_tag="CCEL_RS10675"
                     /old_locus_tag="Ccel_2110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P94530|3.A.1.1.34"
                     /protein_id="WP_015925555.1"
                     /translation="MEQSNRVYSFIMYIFLAVCSVIAIGPFYLMIIMSTYDSNALFSDL
                     HLLPGNNFMINLKDVFLNAGFGRFYFNSIYIAVLSTALTVFVCAMCGYALAKFDFKLKK
                     TAYYAILITMMLPTQLGLIAFVTEMNVIGWTDSHLSLIIPAAANAFAVYWIRQYTVQGV
                     PTEILESGRMDGCTEGGIFFKLVIPFIKPALGSQALLCFMAAWNSYLLPLVLISDPKKY
                     TVTLGLSTLDALYRANYGARIAALVIGTIPMFCIFLVFSKSLIQGITAGSVKG"
     gene            complement(4702..5622)
                     /locus_tag="CCEL_RS10680"
                     /old_locus_tag="Ccel_2111"
     CDS             complement(4702..5622)
                     /locus_tag="CCEL_RS10680"
                     /old_locus_tag="Ccel_2111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P94529|3.A.1.1.34"
                     /protein_id="WP_015925556.1"
                     /translation="MKNLSKKSAPYLFMAPYFLLFLTFSLIPIIFTLYMSLNEWNGYEG
                     IKFVGLANYQRMFENGEFLLALKNTAIIMLLLIPLQLIFAVIIAYMINSKLVRHKEIFK
                     TAVFTPYLVIPIAAGLLWAFFFDGGSSGTINAILMKLHILKEPVDYLASPKLAKVVIAV
                     IMLWRYTGYCVLFFIAGFVSVPEELYEAARVDGARAWHNFWNISLPMIKPIIIYMVITS
                     LIGGFQTFDEPNIIYTQGNYQTGPYSGGPDGAVLTLVMLMGKGAFQNSQYGYGSAVAYG
                     MFVVIAIFSFASLKFMNRGDKDGAI"
     gene            complement(5729..7078)
                     /locus_tag="CCEL_RS10685"
                     /old_locus_tag="Ccel_2112"
     CDS             complement(5729..7078)
                     /locus_tag="CCEL_RS10685"
                     /old_locus_tag="Ccel_2112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="WP_015925557.1"
                     /translation="MFKKVIASVLTGVMVLGMAACGNTESGDSTASASSSAASSSSVAS
                     TSSPAATSTDFTKASGTVVMWNWENQDQLKTAIADFNTRYPNVKVESVPVASADYVKKI
                     QTAVAAKTALPDVIRGEVGFRGTLFDMDICEDLSQAPYNFDKTKLAEKSLPLVTNDKGQ
                     VLGAPTQFNPSGIAYRRSMTKKLFNTDDPKELADKFKTWEDVIAAFQNAKIDGKKVYAF
                     RSVRDIFHIVDGYNPQNPIQDGTIKFNEVYLPTFQVIEKMWKAGVINKYDMWTPAWNAS
                     FAKSEDVFAAAAPWFLKYVIEPNDEKGKGDWGVTVAPGGMFNWGGTALSIWKDSKVKDA
                     AWGYIADQILNENGVKNSFTTGMNITPVKEFIDKPGFFSQKEEYWGGQDVGQFYMDNMD
                     AVKVKSLGKYDNFLENNFVKGLLEIKKGKTAQEAVDFMIADMKKNVPELK"
     gene            complement(7414..9030)
                     /locus_tag="CCEL_RS10690"
                     /old_locus_tag="Ccel_2113"
     CDS             complement(7414..9030)
                     /locus_tag="CCEL_RS10690"
                     /old_locus_tag="Ccel_2113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0036286,DBD-SUPERFAMILY|0035607"
                     /protein_id="WP_015925558.1"
                     /translation="MYKVLIIDDESIIRKGIKNIINWKQLDCEVCADASDGIEGIELIK
                     KYVPEVIITDIRMPGMDGLEMIKNVKEIVPNSKIIILTGYRDFDYVQKAIKCGAYDFLL
                     KPSKIEELTSVLTNAVRELDEQKNKYNEIERFRMLFEQNIPVLREKLLYDIIYGINTSE
                     NEIYEKMKLFDISIKNFVLVVMENDYNDKSNSSLYDKHLYQFGIVKSFEEIFAENYEVL
                     SIMLNSNRVGFIIQKSDNLPLDIEKVSEKCAYLQEVINSGFGFTVTIAVSSNGKEALEL
                     PEKLKECLGSLEYKSYMGDNSIIQYSDLNSFFRYEDYSILDKYQKQLIENIKSGNELGV
                     KENTKLISDYLTNNNINIHYMRNFYYTTLSSINNIRISVSSVDTEKKYSEGEDIASLIK
                     LIEKTDKAEELNSLLEEVAVRIASKVNSFNNKSIKLILRKAIDYIQEHYNEQVTLNEVA
                     ENIYVSTFYISRMFKKELGKSFVDYLNDVRIDKAKELLKDVRYKTYEVAEKVGISDPHY
                     FSKLFKKYSGMTPSEYRDSCY"
     gene            complement(9008..10891)
                     /locus_tag="CCEL_RS10695"
                     /old_locus_tag="Ccel_2114"
     CDS             complement(9008..10891)
                     /locus_tag="CCEL_RS10695"
                     /old_locus_tag="Ccel_2114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="WP_015925559.1"
                     /translation="MINKITSSMFSKMLRSLQFYKNISIKKKLLLVVNLQILIPLIFVG
                     FFAYKSSEEIINSKSLSYSNDVLSSIGLRLQDTVTNLTNISGDISMDKNIYDALISYDI
                     GNNNNNLTETGIREIDSQLTNLFITVKGNRPEINSICIITDSGLVLPRRFPSTDSELRS
                     VVNREYSRMSEKADEVSRKAAWFFETSSGIVKNAYLVKKVYNRDNYTEIGMLVVQIKMD
                     SLRDVLKGLESEVMQNTTILSPNNDIIVSKNKSALEKYKTIIKDTPYDQDSFIDQDNNI
                     FVAYTYLKDNINWRIVSFVPLDELYSDVNKLRNKIILLCFGSIVLLSVVSIYMSFDMIK
                     PINRLVKAMKNMNINNVSDSYIEIDRGDELGFLHKTFNNMAKEIDHLVTWIYREQITRK
                     EAELKALQSQINPHFLFNTLESINWMAQLNNVPEISNIVTDLSALLEASIGRDDRLIPI
                     EEEFMYIDKYISLLKRRFEDKITLKKEIDPQVLYIKIPRLLIQPLIENAVYHGVESSRE
                     KGVIMLNARIQGDTILLEVIDNGNGISKEDLLKLNKSLEMDNDTYFKSLREKKNKSIGI
                     DNVNRRIKLFYGEKYGIKIESSINIFTKVTVCLPLQTFNTKESYYVQGSNN"
     gene            complement(10946..12244)
                     /locus_tag="CCEL_RS10700"
                     /old_locus_tag="Ccel_2115"
     CDS             complement(10946..12244)
                     /locus_tag="CCEL_RS10700"
                     /old_locus_tag="Ccel_2115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="WP_015925560.1"
                     /translation="MQSLKKLLCLVLFTLVSIISVSCSSDNSYVSNARNSKTTQAETST
                     ISLMTSWGGVDSKAGCLMDLLDRFENGNPSIKVSNQSIFGDEYLPTLKTRFASGNEPDV
                     FGLWPCSDIKYMIMANKLADLTDMLTKDSEWMDSFKGNYFDLTTYNNRIYGIPFELVFE
                     GMFINKDLFQQFNVKIPQNYEELKNAINIFNKHNITPIAYNATAEGSYIYQNMIASLGG
                     NDGVENYMVNNQINKCYIDAMKYMKELHKMHAFPTDLISITSEERNNLFIKKQAAMIVQ
                     GSWFAAYFGKFDKTVEMIPFPSMGNGNRKIPAGLGGGTFYISKSAWGTPNSKENTVKLL
                     KFLTSKETSDYLYKESGLFSTLNISRETPFNALAKQSIDIYENTPEQDRCAIPDHVIDR
                     STWEKIIVKEFPDYLDDKISAEQIWEKALDKLQ"
     gene            complement(12445..14790)
                     /locus_tag="CCEL_RS10705"
                     /old_locus_tag="Ccel_2116"
     CDS             complement(12445..14790)
                     /locus_tag="CCEL_RS10705"
                     /old_locus_tag="Ccel_2116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9FI56|3.A.9.1.2"
                     /protein_id="WP_015925561.1"
                     /translation="MIKCSICNKNIAVVFVTKIIDGKQIQEGLCVSCAKKQNLQPIDQL
                     LSQTGMTEYELENISKQVGDMLEEMDGSEIMEAMQVDPENINQANPFFSILNKAFPKTG
                     DSELNNSNKNIQPVGQDKNGEDKDRNYTKTKAQDKKNVKKKKYLEMYGTNLIDKAKEGS
                     IDNIIGRTREIERVIQILNRRTKNNPVLIGEPGVGKTAIAEGLALRIAERSVPAKIQNS
                     EVYLLDMTSVVAGTQFRGQFESRMKGIIDEAKSFGNIILVIDELHNIMGAGEAEGAMNA
                     ANILKPALSRGEIQVIGATTLNEYRKHIEKDTALERRFQPIIVDEPSIEESIEILRGIK
                     HYYEQYHRVKINDEIVKAAVNYSERYITDRLLPDKAIDVIDEAGSRANLRNTKLAELQS
                     YKDELKKVQEEKEFAVSADSIEDYQKAADLKVYECKLQELIKDIEDTSKDVELTVEDIA
                     AVIEAWTKIPIQRLSEGESEKLMSLESRIHQRVIGQEKAVEGVAKAIRRSRSGFKKKKK
                     PSSFIFVGPTGVGKTELVRALSTELFGSEEALIRLDMSEYMEKHTVSKLIGSPPGYVGY
                     DDAGQLTEKVRRRPYSVILLDEIEKAHPDVFNMLLQILEDGRLTDSHGKTVNFENTIII
                     MTSNAGTNFKSGGIGFANNTYIALESRVRDVIKETFRPEFLNRIDEIIVFTELGTDELK
                     KIIDLMLEEVYQEASEKDIRVNVSDKVKEFILEKGYDPKFGARPLRRTVQNYIEDRLSE
                     EYLKGTMKEGSLVGIDIDENNEVVLKIV"
ORIGIN
        1 ctaccccttg aaggggtctt catactcttt cacgcttagt ttatctacca taatatcgtg
       61 cttttcctgc tcttggatat actttctaat agttgcttca ttaagcccta cagtactcac
      121 atagaagccc tctgcccaaa aatgtctatt tccgaactta tactttaggt ttgcatgctt
      181 atcgaatatc atcaatgcac tttttccctt aagataaccc ataaatgttg aaactgatat
      241 ctttggtggt atacttacta acatgtgtat atgatctggc attagatgcc cttcaagtat
      301 ttctactccc ttatacttgc ataatcgtat caatatttct cgtatgcttt gtttgtattg
      361 attgtatatt acttttcgtc tatactttgg agtgaatact atgtgatact tacacatcca
      421 tttggtatgt gctaaactat tttctttatt tgccattaaa atcacctttt ccctcgttaa
      481 tctagcagct tgaacaactc tattttaacg aaaaggtgat ttttttgtat aactactgtc
      541 gcgcacccgc atagcgggtg gtttattatt tcgtatgtct ccgtttctct gttgagaaac
      601 tccaacatac tcaacaggct aaagcccata acaacaaagc agccttctat tgtatagcct
      661 gtacaatagg aggctgcttt gcgtgcatat tgaggaatct atttttaaat acagaaatta
      721 tcccattatt acttcgacct gatgtgtctt ttcatcatta aatataggaa ttatattacc
      781 acctatttcc ttcccatcta caactacttt cttaactcct tttgaaacgt gttcgggatt
      841 gctaataaca atttcaaaaa ctgcatttct gaattttctg gttatcttgt aaccatccca
      901 acttgctggt atacacggat ctatctttag tcctgaataa tcaggcttta taccaagtat
      961 attttgtgag attactacaa agttccatgc tgctgtacca gtaagccaag aattctttgc
     1021 ttctcccggt ctcttagcat ccttacctgc aatcatctga gaataaacat aaggttctgt
     1081 tttgtgaata tcacttatct cctcagtata tgcaggagca attttggaat aatattcaaa
     1141 agctctgtcg cctctgccta aaactgtttc acctgcaata atccaaggat tattgtggca
     1201 gaatatgcct gcattttctt tatatcctgc aggatatgta gatatctcac ccatgttgac
     1261 atagtatttt gtaaatgccg gattttgaag tacaagtccg tacggagtat ccaaatactt
     1321 tcttgcagaa tccaaggctt tctcgacata accgtcttcc agacctatct ccgccataga
     1381 acagaagccc tgtgattcaa tgaaaatctt accttcttca ttttcattac ttccaatctt
     1441 ctcactgtta tcatcgtaag cacggataaa ccattcgccg tcccaaccat actccttaac
     1501 agtttttatc attttatcaa tttcaacttg agccttagca gcttcatctt caagtccatt
     1561 aagctcacaa agttttacgt actccggacc gtagtaaaca aacattccgg ctatcaaaac
     1621 agactctgcc actttcccat ctttacttgt ggttgtctgg aatgattcgt ctggagtctc
     1681 ggagaaacag tttagattga gacagtcatt ccagtctgcc cttcctataa gaggcagtcc
     1741 atgaggtcca agattgttca caacatgata gaaagacctt cttaagtgtt caaaaagaga
     1801 agcagctttt gtgtcatcgt tgtcaaaagg aacattttcc tttagtatgt ccatatctcc
     1861 tgtctcttta atatatgcag ctacagaagc aataagccac accgggtcgt cattaaaatt
     1921 tcccccaatt tcgttgttac ccttctttgt aagcggttgg tattgatgat atgcaccacc
     1981 gttttccaac tgggttgcag ctatgtctag gatcctttct ctagctctgt caggaatctg
     2041 gtgtacaaat cccaatatat cctgatttga gtctctaaaa cccatacccc ttccgatacc
     2101 tgtttcaaaa tatgaagcac ttcttgacat attaaatgta accatacatt gatactgatt
     2161 ccatatgttc accattctgg acagtttctc atctttgtgc tcaagtttat actgtgtaaa
     2221 caactggctc caataattct gcagttcatc aaaagctgtc tgaactccgg caggattacc
     2281 tttttcttct atcatcttat atgctttctt tttatttata acgcctttac tttcccattt
     2341 ttcatttaca tcgttttcaa cataacccag aatgaaatca agttcctttt cttcaccagc
     2401 tttcaaatca acggttatgc aatgtgatgc catagggctc catccgtggg caatggtgtt
     2461 ttttggttca ccgctgacag ggacctgtgg agcgtcaaaa ccattgtata agccaataaa
     2521 ctcatctcta tccgtatcaa agcctgttaa gtcagcattg acagagtaaa aggaaaaatg
     2581 gtttcttctt tccttatatt ctgttttatg gtatattacc gagttctcaa cttcaacctc
     2641 acctgtactt aggtttctct gataatttgt catgtcgtca taggcattcc ataagcagaa
     2701 ttcaatacat gagaataact taacactttt attatcagat gttgtattct taactcttac
     2761 acgatgaatt tcaccattaa agttcaatgg tacaaaatat aaaacctctg tactgatacc
     2821 gttcttactt ccggttattt ttgtataccc caaaccatgc ctgcactcat atttatccaa
     2881 atctgcctta actggagacc atcccggtga ccagagaacg ccattgtcgt ttatataaaa
     2941 atatcttcct cccatgtcta tcggaacatt attatatctg taacgggtta ttctacgtaa
     3001 acgtgcgtcc ttataaaaac aataaccgcc tgcagtattt gatatcaaag agaagaactc
     3061 ctgagttccc agataattaa tccaaggata aggtgtttta ggtgttgtaa tgacatattc
     3121 tctatttgta tcatcaaaga aaccgtattt caaatttata acccctttcg atgtcgaatt
     3181 attttatgag aaacctcttc tatttacttt gcaactgttt ctctaataat cattatttta
     3241 aatattttta attagaactt ttttctttac ttttttaaat atgtaataca taaaaaaatt
     3301 gtactaagga acatgcgtaa ttgaaagata agtgtcagaa aaaggaatca gtatttatat
     3361 taaaatcaaa aagacggggg ccccttttta gagacattat atgaacatta gcttctctta
     3421 acaattcttc tcccccgcct atttctactt tttaacaaat actgttacta tactaatata
     3481 ttgtttagac aacatataaa acaatactct atattccagc attgtataaa tgtcgatgcc
     3541 attttttcgt acaaaaagtt taataatgca actttttgta gaaaatcatt ctatttatat
     3601 tttattcaaa ttaagaaaaa cttgaatgga acaacttgct ttaatgtgca aaattttgtc
     3661 attgcttagt ataatagata taaacccata tttacaaact tacattatcc agttaatttt
     3721 ctttgagcag tcgaaaatct gctcaaagag taatttaacc agttgtttta aaacgctgaa
     3781 taccttttaa aaaggtattc aagacaaaac cttttataaa aattacatgt tcggtatctt
     3841 acatctttct atcaaatcaa taggaactct gtacataaac ctgcaagtta gccttttaca
     3901 gaccctgcag taataccttg aataagtgat tttgagaata ccaggaatat gcagaacata
     3961 ggtattgtac caatgaccaa tgctgctatt ctggcaccat agtttgctct gtacaacgca
     4021 tccagtgttg ataatccaag agtaacagta tacttcttcg ggtcactgat taataccagc
     4081 ggtaacagat atgaattcca agctgccata aagcataaca aagcctgtga gccaagtgcc
     4141 ggcttaataa atggtataac caacttgaag aaaattcccc cttcagtaca accgtccatt
     4201 cttccgctct ccagtatttc agttggaacg ccttgaactg tgtattgtct tatccaataa
     4261 actgcaaatg catttgcagc tgcaggaatg ataagtgaca aatgtgaatc agtccaacca
     4321 atcacgttca tttcagtaac aaatgctatc agacccaact gagtaggaag catcatagta
     4381 atcaaaattg cataatatgc tgtctttttg agcttaaagt caaatttggc aagtgcatat
     4441 ccgcacatgg cacatacaaa tactgtcaat gctgttgaga ggacagcaat gtaaatactg
     4501 ttaaaataga atctcccaaa ccctgcattt aggaatacat ccttcaaatt tatcataaag
     4561 ttgtttccgg gcaataaatg aaggtcagaa aaaagagcat ttgaatcata ggtactcatt
     4621 attatcatga gataaaaagg acctatagca attactgaac aaactgctag aaatatatac
     4681 ataataaagg aataaactct attagattgc tccatcttta tcccccctat tcatgaactt
     4741 taatgaagca aatgagaata ttgctattac tacaaacatt ccatatgcaa cagcactacc
     4801 ataaccatat tgagagttct ggaacgcacc cttacccata agcattacca aagtcaaaac
     4861 tgcaccatca ggtccccctg agtatggtcc cgtttgataa tttccctgag tataaataat
     4921 atttggttca tcaaatgtct ggaaaccacc tataagggat gtgataacca tatatattat
     4981 tataggcttt atcattggaa gagaaatgtt ccagaaattg tgccatgctc tggctccatc
     5041 aacacgtgca gcctcataca attcctccgg tactgataca aaaccggcta taaagaacag
     5101 tacacaatat ccggtgtatc tccacaacat gataactgca ataactactt tagctaattt
     5161 gggacttgca agataatcaa ccggctcttt aagaatatgc aatttcatta agattgcgtt
     5221 gattgttcct gatgaacctc catcgaagaa aaatgcccac aacaaacctg cagctattgg
     5281 tataacaaga tatggagtaa aaaccgcagt cttaaaaatt tctttgtgcc tgacaagctt
     5341 tgaattaatc atataggcaa taattactgc aaatatcaac tgcaacggta tcaacagcaa
     5401 catgataatc gcagtatttt tcaaagctag aagaaactcc ccattttcaa acattctttg
     5461 ataatttgct aatcctacaa attttattcc ctcgtatccg ttccattcat taaggctcat
     5521 atacaaagta aatattatag gtattagaga aaatgttaga aaaagaagga agtaaggtgc
     5581 catgaaaaga taaggtgctg atttctttga gagatttttc attactaacc acccccgtgt
     5641 gcttgattaa gtacaaacag taaaataatc aaggagaggg aatttcaccc tctcctaaat
     5701 cataaataac attcagataa ttccgaaatt acttcaattc aggaacattt ttcttcatat
     5761 cagcaatcat gaaatcaact gcttcttgag ctgtctttcc ttttttgatt tccagtaatc
     5821 cttttacgaa attattttca aggaaattgt catatttacc gagtgattta actttaactg
     5881 catccatgtt atccatgtag aattgtccta catcctgtcc gccccagtat tcttcttttt
     5941 ggctgaagaa acctggttta tcaatgaatt ctttaacagg agtaatattc ataccagttg
     6001 taaatgagtt cttaacaccg ttttcattaa gtatctggtc agcaatgtat ccccacgctg
     6061 catccttaac ctttgaatcc ttccagattg acaatgcagt accaccccag ttgaacattc
     6121 cgcccggagc tactgttact ccccagtcac ctttaccctt ttcatcgttt ggttctataa
     6181 cgtacttgag gaaccaaggt gctgctgctg caaatacatc ctcgctcttt gcgaatgatg
     6241 cattccatgc aggagtccac atgtcatatt tgttgataac gccagccttc cacatctttt
     6301 ctataacttg gaaagtagga agatatactt cgttaaactt gattgttcca tcttgaattg
     6361 ggttttgagg attatatcca tcaactatgt ggaatatatc tcttactgat ctgaatgcgt
     6421 aaaccttctt gccatctatc ttagcattct ggaaggcagc gattacatcc tcccaagtct
     6481 taaacttgtc tgccaattct ttcggatcat cagtattgaa taatttcttt gtcatgcttc
     6541 ttctatatgc aatacctgat gggttgaact gtgttggagc tcctaatacc tgtcccttgt
     6601 catttgtaac aagaggtaat gacttttcag ctaactttgt cttgtcaaag ttgtatggag
     6661 cttgtgaaag atcttcacaa atatccatgt caaagagggt tcctctgaaa ccaacttctc
     6721 ctctgattac atctggtaaa gctgtttttg ctgcaactgc agtctgtatt ttcttaacat
     6781 agtctgcact tgcaactggt actgattcaa ccttaacatt tggatatctg gtattaaaat
     6841 cagctattgc tgtcttaagc tgatcctgat tttcccagtt ccacattact accgttccag
     6901 aagcctttgt aaagtctgtt gatgtagcag ctggtgaaga agtagatgct actgaactac
     6961 tgctagctgc tgagcttgat gcagaagcag tactgtcacc tgactctgtg tttccacaag
     7021 cagccattcc cagtaccata acgcctgtta aaacagaagc tattaccttt ttaaacattt
     7081 aaaaatcctc ctttttattt ttggcatttt catattgaaa tgcttacatt agtgccatta
     7141 aagcactttt caacattaat tctataaaaa tagattctct ttctaaatgg ataatgttgc
     7201 tttatgtttt aatattttga ctatatgggt atggcaagaa attgcacaaa aaagacacat
     7261 attattacaa atatgtgtct tttttgacga attgtattat tttttaaatt tacgggtcta
     7321 attatgttta tatccacagc atggttacct acaattattt ccattaaagg tcaatattga
     7381 acaccatgtt atagcattat taattagtat gaattaataa caactatctc tgtattccga
     7441 gggcgtcatg cctgaatact tcttgaataa ctttgaaaaa tagtgaggat cagaaatacc
     7501 aaccttctct gcaacttcgt aggttttata ccttacatcc tttagaagtt cttttgcctt
     7561 atcaattctc acatcgttaa ggtaatctac aaagcttttt ccaagttctt ttttgaacat
     7621 tctgctaata taaaaggtac taacatatat gttttctgct acttcgttaa gtgttacctg
     7681 ttcattgtaa tgttcttgta tataatctat agctttacgt aatatgagct ttatgctctt
     7741 attgttgaag ctgttaacct tggatgcgat tctcacggct acttcttcca atagtgaatt
     7801 aagttcttct gccttatcgg ttttttctat aagctttatg aggcttgcta tatcctcacc
     7861 ttctgaatat ttcttctccg tatctaccga cgaaaccgat attcttatgt tatttattga
     7921 agacaacgtt gtataataaa aattccgcat ataatgtata tttatattgt tgtttgtcag
     7981 ataatcagat ataagctttg tattttcctt tactccaagc tcattccctg attttatatt
     8041 ttcaataagc tgtttttgat acttgtccag tattgagtaa tcttcatatc tgaaaaatga
     8101 gttcagatca ctgtattgaa ttatagagtt gtctcccata taactcttat attccaatga
     8161 gcccagacac tcctttaatt tttctggaag ctccagagct tcttttccgt ttgaactgac
     8221 agctattgta acagtaaaac caaatccgct attaataacc tcttgaaggt aagcacattt
     8281 ttcacttacc ttctcaatat ccaaaggtaa attatcagat ttctgtatta taaaacctac
     8341 tctgttactg ttgagcataa tgcttaaaac ttcataattc tctgcaaaga tttcttcaaa
     8401 ggattttaca ataccgaatt ggtaaaggtg tttatcgtat aacgaactgt ttgatttatc
     8461 gttgtagtcg ttttccatta caaccagaac aaaatttttt attgatatat caaacagctt
     8521 cattttttcg tatatttcat tttcacttgt gttaattccg tatataatat cataaagcag
     8581 cttttctcta agtacgggaa tattttgttc aaaaagcatc ctgaaccgtt ctatctcatt
     8641 atacttgttc ttctgttcat ctaattccct gactgcatta gtcagcacag aagtcagttc
     8701 ttcaattttt gagggtttca ggaggaaatc atatgccccg catttaatag ccttctgcac
     8761 atagtcaaaa tccctgtaac ccgtcagtat gatgattttt gagttaggga caatttcctt
     8821 tacgttcttg atcatttcaa gaccatccat tccaggcata cgaatgtcag ttataattac
     8881 ttcaggtaca tatttcttta ttagctcaat tccttcaatg ccgtcactgg catctgcaca
     8941 aacctcacaa tcaagctgtt tccaattaat aatattctta atgccttttc gtataataga
     9001 ttcatcatca attattagaa ccttgtacat aataactctc cttagtattg aatgtttgaa
     9061 gcggtaaaca aaccgttact ttagtgaata tgttaatact gctctctatt ttgataccgt
     9121 atttttcacc atagaagagt ttgattcttc tatttacgtt atctattcct atgcttttat
     9181 ttttcttttc tctaagagat ttaaaatatg tgtcattatc catttcgaga ctcttattta
     9241 actttagaag gtcttctttt gatattccgt taccgttatc aattacttct aatagtatcg
     9301 tatccccctg tattctggca tttagcataa taacgccttt ttccctgctg ctttccactc
     9361 catggtagac agcattttcg attaacggtt gtatcagaag tcttggaatc tttatatata
     9421 aaacttgagg atctatttct tttttcagcg ttattttatc ttcaaatctt cgttttaaga
     9481 gagatatgta tttatctata tacataaact cctcttctat gggtataagc ctgtcgtccc
     9541 ttccaatact ggcttcaagc agtgcagaca ggtcagttac tatattgctt atctcaggaa
     9601 cattatttaa ttgtgccatc caatttatgg attccagtgt attaaacagg aaatgagggt
     9661 ttatttgtga ttgtaatgcc tttaattccg cctctttgcg ggtaatctgc tccctgtaaa
     9721 tccatgtaac aaggtggtca atttctttag ccatattatt gaaggtttta tgcaggaagc
     9781 ccagttcatc acctctgtca atctctatat aactatccga aacgttgtta atgttcatat
     9841 ttttcatagc ttttaccaat ctgtttattg gttttatcat atcgaaagac atgtaaatgc
     9901 ttactacaga cagcaggact atagacccga aacaaagcaa gattatcttg tttctcaatt
     9961 tatttacatc cgaatataat tcatctaaag gaacaaacga aactatcctc cagttgatat
    10021 tatcttttaa atatgtatac gcaacaaaaa tattattatc ttgatctata aaagaatctt
    10081 gatcatacgg cgtatccttg attatggttt tatatttttc caatgccgat ttatttttgg
    10141 aaacaattat atcattgttt ggtgatagta ttgtagtatt ttgcataacc tcggattcca
    10201 gtccctttaa aacatctctc aaggagtcca tttttatttg gacaaccaac attcctattt
    10261 ctgtataatt gtccctgttg tataccttct tgaccaaata agcattcttt actatacccg
    10321 aagaagtttc aaagaaccag gctgcctttc tgcttacttc gtcagccttt tcactcattc
    10381 tggagtattc cctatttacc actgacctga gttccgaatc agtacttgga aaccttcttg
    10441 gtaaaactaa gccgctatca gttataatac aaatagaatt gatttctggt ctgttaccct
    10501 ttacagttat aaataggttg gttaactggg aatctatttc tcgaattccc gtttctgtta
    10561 aattattatt gttattacct atatcatagc tgattaaagc atcgtatata ttcttatcca
    10621 tacttatatc accggatatg ttagtcaagt ttgttaccgt atcctgaagc cttaatccaa
    10681 tagagctaag tacatcgttt gagtacgaaa gggatttact gttaataatt tcttcagaac
    10741 tcttatatgc gaaaaatcct acaaatatta ggggtattaa aatttgcaga tttactacga
    10801 gcaatagctt cttctttatt gatatgtttt tgtaaaattg taaacttcta agcatcttag
    10861 aaaacatact tgaagtgatt ttgtttatca ttcttccacc atatcctaat aaacatgagt
    10921 aattttatta aatgttgcta tttttttact gcaatttgtc taatgctttt tcccatattt
    10981 gttccgcaga tattttatca tcaagataat ctggaaactc cttgacaata attttttccc
    11041 aagtacttct atctataaca tgatcaggga ttgcacaccg gtcttgttca ggagtattct
    11101 catatatatc tatactttgt tttgccaatg cattaaaagg ggtttccctt gatatattta
    11161 gtgtgctgaa taggccggat tccttgtata aataatctga tgtctctttt gacgtaagga
    11221 atttcaaaag ttttacggta ttttctttgc tattgggtgt accccaggca cttttactta
    11281 tataaaaggt tcccccgcct aaacctgctg gtatttttct gtttccattt cccatggacg
    11341 gaaaaggtat catctcaaca gtcttatcaa actttccgaa gtatgctgca aaccatgaac
    11401 cctgaactat catggctgcc tgttttttta taaaaaggtt gtttctttct tcgctggtaa
    11461 tagagattaa atcagtcgga aatgcatgca tcttgtgaag ttccttcata tacttcatag
    11521 catcaatata acatttgttt atctggttat taaccatata attttctact ccatcattgc
    11581 cgcctagact ggcaatcata ttctgatata tatatgaacc ttcggcggta gcattatagg
    11641 ctataggcgt tatattatgt ttattaaaga tatttatagc gtttttaagc tcctcatagt
    11701 tttgaggtat ttttacatta aattgctgaa ataagtcctt attaataaac atcccttcaa
    11761 atacaagctc aaaaggtatc ccatatatcc tgttgttata ggtagtcaag tcaaagtaat
    11821 ttcccttaaa gctatccatc cactcactgt ctttcgtaag catgtccgtc aggtcagcaa
    11881 gcttatttgc catgatcata tactttatat ccgaacatgg ccataatccg aaaacatcgg
    11941 gttcatttcc tgatgcaaac ctggttttaa gagtaggtag atattcgtct ccaaaaatag
    12001 attgattaga aacttttata gatggatttc cattttcaaa tctatcaagc aaatccatca
    12061 agcagccggc tttactatca acacctcccc aacttgtcat taaacttatt gtactggttt
    12121 cagcctgagt agttttgctg ttacgagcat ttgaaacata cgaattgtca ctgctgcaag
    12181 aaactgatat tatacttact agtgtaaata gtactaaaca taaaagtttt tttaagcttt
    12241 gcatttgttt cactccaatt cttatttaat atagccatgt tttgtctact attaagaacg
    12301 agcataacat atcatataca attataaata ctaataggta tatattgcaa ggatttacaa
    12361 ttaattatcg acgtgatgtt ttgtaatatg taacaagtaa aatagcagca agagctgaat
    12421 cctgctgcta tttatataaa attattaaac aatttttaaa acaacttcat tattttcatc
    12481 aatatcaatc cctaccagtg aaccttcctt catcgtacct tttaaatact cttctgacag
    12541 gcggtcctca atgtagttct gtactgttct tctcaaaggt ctggcaccaa actttggatc
    12601 atatcctttt tccagtatga attccttaac tttatctgaa acattcactc tgatatcctt
    12661 ttcactggct tcctgatata cctcttctaa catcagatcg attattttct ttagttcatc
    12721 agttccaagt tcagtgaata caattatctc atctattctg ttcaggaact ccggcctgaa
    12781 agtttctttt attacatctc ttaccctgct ttcaagtgca atataggtat tatttgcaaa
    12841 acctatgcca ccggatttaa aattagttcc tgcatttgat gtcataataa taatagtatt
    12901 ctcaaagttt acagtctttc cgtgactgtc tgtaagcctt ccgtcttcaa gaatttgaag
    12961 aagcatatta aaaacgtccg ggtgggcctt ctcaatttca tccaacagta ttactgaata
    13021 cggtcttctt cttactttct ccgttaattg acctgcatca tcataaccca catatcccgg
    13081 aggtgaacct attaattttg atactgtatg cttttccatg tattctgaca tatcaagcct
    13141 tattagggct tcttcgcttc caaaaagttc tgttgaaaga gctcttacca gctcggtctt
    13201 acctacacca gtgggaccga caaaaataaa ggaggatggc ttcttcttct ttttgaaacc
    13261 tgacctactt cgtcttattg cctttgcaac gccctctaca gctttttcct gtcctattac
    13321 cctctgatga atacgtgatt caagactcat aagcttttcg gattcaccct cactaagtct
    13381 ttgaataggt atctttgtcc atgcttctat tactgctgct atgtcctcca cagtaagttc
    13441 tacatcttta cttgtatcct ctatatcttt aataagctcc tggagcttac attcataaac
    13501 ctttaaatca gctgccttct ggtaatcttc tatggaatca gctgaaaccg caaattcctt
    13561 ctcctcttgt actttcttca gttcatcctt gtatgattga agctcagcaa gctttgtatt
    13621 tctcaggttg gctctggagc ctgcttcatc tattacatca atggctttat caggtaaaag
    13681 tctgtcggta atataccttt cagagtaatt tactgcggcc tttactatct catcatttat
    13741 tttaactcta tggtattgtt catagtagtg cttgatgcct ctaagaattt ctatactttc
    13801 ctcaatggat ggttcgtcaa caataatagg ctggaatctg cgctctaaag cggtgtcctt
    13861 ttctatatgc tttctgtatt cgtttagcgt tgtcgcacca atcacctgaa tttcacctct
    13921 ggataaagcg ggctttaaaa tgttagcagc attcatagct ccttcagcct cgccggcacc
    13981 cattatatta tggagttcat caatgacaag aattatattt ccaaacgatt ttgcttcatc
    14041 gattattccc ttcatcctgc tttcaaactg tcctctgaat tgtgttccgg caaccacact
    14101 ggtcatatcc agaaggtaaa cctcagaatt ctgtattttt gcaggaacgg atctctcagc
    14161 tattctcaaa gccagacctt cagcaatagc tgttttacct acacccggtt ctcctatcaa
    14221 tacaggatta ttttttgttc ttcgattcaa aatttgaatt actctttcta tctcacgagt
    14281 tctgcctatg atattatcta tgctaccttc tttggcctta tctataaggt ttgtcccata
    14341 catttcgaga tattttttct ttttaacatt ctttttgtcc tgagctttgg tttttgtata
    14401 attcctatcc ttatcttccc catttttatc ctgaccgact ggttgaatgt ttttatttga
    14461 attgtttaac tctgaatcac ctgtctttgg aaatgcctta ttcaatatac tgaaaaaggg
    14521 atttgcctga ttaatatttt cagggtctac ctgcattgcc tccattattt cagacccatc
    14581 catctcttca agcatatctc ctacttgttt gcttatattt tcaagctcat attctgtcat
    14641 tccggtttgt gacagtaatt gatctattgg ctgtaaattt tgtttttttg cacagcttac
    14701 gcataagccc tcctgaatct gttttccgtc tatgattttt gttacaaaga caactgcgat
    14761 gtttttattg caaatggagc attttatcat
//
