LOCUS       NC_009004               4738 bp    DNA     linear   CON 17-FEB-2020
DEFINITION  Lactococcus lactis subsp. cremoris MG1363, complete sequence.
ACCESSION   NC_009004
VERSION     NC_009004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3138204
            Assembly: GCF_000009425.1
KEYWORDS    RefSeq; complete genome.
SOURCE      Lactococcus lactis subsp. cremoris MG1363
  ORGANISM  Lactococcus lactis subsp. cremoris MG1363
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Wegmann,U., O'Connell-Motherway,M., Zomer,A., Buist,G., Shearman,C.,
            Canchaya,C., Ventura,M., Goesmann,A., Gasson,M.J., Kuipers,O.P., van
            Sinderen,D. and Kok,J.
  TITLE     Complete genome sequence of the prototype lactic acid bacterium
            Lactococcus lactis subsp. cremoris MG1363
  JOURNAL   J. Bacteriol. 189 (8), 3256-3270 (2007)
   PUBMED   17307855
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Wegmann,U. and Linke,B.
  CONSRTM   MG1363 Sequencing Consortium: Institute of Food Research (Norwich,
            United Kingdom), Groningen Biomolecular Sciences & Biotechnology
            Institute (Groningen, The Netherlands), Alimentary Pharmabiotic
            Centre and Department of Microbiology (Cork, Ireland)
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) Wegmann, U., Institute of Food Research,
            Food Safety Science Division Norwich Research Park, Colney, Norwich
            NR4 7UA, England Linke, B., Center for Biotechnology, Bielefeld
            University Universitaetsstrasse 25, 33615 Bielefeld, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2020 12:52:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,549
            CDSs (total)                      :: 2,463
            Genes (coding)                    :: 2,317
            CDSs (with protein)               :: 2,317
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 73 of 146
            Pseudo Genes (incomplete)         :: 48 of 146
            Pseudo Genes (internal stop)      :: 42 of 146
            Pseudo Genes (multiple problems)  :: 16 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            AM406671.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Lactococcus lactis subsp. cremoris MG1363"
                     /mol_type="genomic DNA"
                     /strain="MG1363"
                     /sub_species="cremoris"
                     /db_xref="taxon:416870"
     gene            1..327
                     /locus_tag="LLMG_RS02225"
                     /old_locus_tag="llmg_0437"
     CDS             1..327
                     /locus_tag="LLMG_RS02225"
                     /old_locus_tag="llmg_0437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9CIF0|4.A.3.2.4"
                     /protein_id="WP_011675488.1"
                     /translation="MADKVIALACAAGMSTSLLVSKMQKAAAENGKDYEIFAKSTADID
                     NMLAGTGSPKPDVLLLGPQVAFMKGEVAKKAEIAGVPMDVIKMQDYGMMRGDKVLAAAE
                     NLMN"
     gene            410..760
                     /locus_tag="LLMG_RS02230"
                     /old_locus_tag="llmg_0438"
     CDS             410..760
                     /locus_tag="LLMG_RS02230"
                     /old_locus_tag="llmg_0438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9CIE9|4.A.3.2.4"
                     /protein_id="WP_011675489.1"
                     /translation="MTDKYENPTSDDYMGVVMGIIMSGGNAKGLAFQAIQQAKDGKFAE
                     AESSLNEASEQLREAHDVQTDLLTRLAQGEKIGWNLYMVHAQDHLMNAITFKDLAVEVV
                     GQERRLQALENK"
     gene            854..1582
                     /locus_tag="LLMG_RS02235"
                     /old_locus_tag="llmg_0439"
     CDS             854..1582
                     /locus_tag="LLMG_RS02235"
                     /old_locus_tag="llmg_0439"
                     /function="Transcriptional regulators"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|HTH_6,STP|SIS"
                     /protein_id="WP_011834482.1"
                     /translation="MSFFGNVDFQKLTYTERTCYSYLRDNVDKIPYLRVRDIALEAHVG
                     TSSVMRLIHKMGYDSYTDFKEYIIDKKELEKGISNTAIPFSSDIFSGDVEQRLDNLAQR
                     VIESDNIIFTGVGSSGLICDYAARRLAGVGINTFSFSDVTYPIASKLQNTTNTLVIALS
                     ISGETNEIIEVLTSLRSNKDVYISSITPKINSSIAELSDFVLTYRINEHRINTHYDLTR
                     QLPTVYLTERLTDLVYQRSN"
     gene            1875..3212
                     /locus_tag="LLMG_RS02240"
                     /old_locus_tag="llmg_0440"
     CDS             1875..3212
                     /locus_tag="LLMG_RS02240"
                     /old_locus_tag="llmg_0440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q72XQ0|4.A.3.2.8"
                     /protein_id="WP_011675491.1"
                     /translation="MNNFIQNKIMPPMMKFLNTRAVTAIKNGMIYPIPFIIIGSVFLIL
                     GQLPFQAGQDFMNKIKLGPLFLQINNASFGIMALLAVFGIAYAWVRDAGYEGVPAGLTG
                     VIVHILLQPDTIHQVTSVTDPTKTSTAFQVGGVIDRAWLGGKGMVLSIIVGLLVGWIYT
                     GFMRRNITIKMPEQVPENVAASFTSLVPAGAIITMAGVVHGITTIGFNTTFIELVYKWI
                     QTPLQHVTDGPVGVFVIAFMPVFIWWFGVHGATIIGGIMGPLLQANSADNAALYKAGHL
                     SLSNGAHIVTQSFMDQYLTVTGSGLTIGLVIFLLVSAKSVQGKTLGRMEIGPAVFNINE
                     PILFGLPIVLNPILAIPFILAPLISGILTYLVIYLGIIPPFNGAYVPWTTPAVLSGYLV
                     GGWQGMVWQIIILALTTVLYWPFAKAYDNILLKEEAETEAGINAAE"
     gene            3302..4738
                     /locus_tag="LLMG_RS02245"
                     /old_locus_tag="llmg_0441"
     CDS             3302..4738
                     /locus_tag="LLMG_RS02245"
                     /old_locus_tag="llmg_0441"
                     /function="Beta-glucosidase/6-phospho-beta-
                     glucosidase/beta -galactosidase"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH1"
                     /protein_id="WP_011834483.1"
                     /translation="MTLKKDFLWGGAVAAHQLEGGWNAGGKGVSVADVMTAGSNGVERK
                     ITDGVIEGENYPNHEAIDFYHHYKEDVALFAELGLNCFRTSIAWTRIFPKGDESEPNEE
                     GLQFYDDLFDECLKNGIEPVITLSHFELPYHLVTEYGGFRNRKLIDFFVHFSEVVMNRY
                     KDKVKYWMTFNEINNQANFMRDFAPFTNSGLKFPEGASEKEREEIMYQAAHYELVASAK
                     VVELGHKINPDFQIGCMIAMCPIYPATCKPEDMMASTVAMQRRYWFADVHVRGHYPSYL
                     KAYFERKEFKLDISDEDLEVLKNGKVDYIGFSYYMSFAIKDHGKAPTFDYNEDKDLVKN
                     DYVKASEWGWQIDPLGLRYAMNWFYERYEKPLFIVENGFGAVDEVEADGSIHDPYRIEY
                     LKAHIESMKEAVEYDGVDLMGYTPWGFIDLVSAGTGEMKKRYGVIYVDKDNEGNGTLKR
                     SKKDSFDWYQKVIATNGEEL"
ORIGIN
        1 atggcagata aagttattgc acttgcatgt gcagccggta tgtcaacatc acttttagta
       61 tcaaaaatgc aaaaagcagc agcagaaaac ggaaaagatt acgaaatttt cgcaaaatca
      121 acagcagaca ttgataatat gcttgcagga acaggttcac caaaaccaga cgttttactt
      181 ttaggcccac aagttgcctt catgaaaggt gaagttgcta aaaaggcaga aattgctggc
      241 gttccaatgg acgtgattaa aatgcaagat tacggaatga tgcgtgggga taaagttctc
      301 gcagctgctg aaaatttgat gaattaagct ttgttttatt aaacctgtgg gagggcgaaa
      361 gctcttttac tatgtaaaaa taagtataaa cagaaacgga atataagaaa tgactgataa
      421 gtacgagaat ccaacaagtg atgactacat gggtgtggta atgggcatta tcatgagtgg
      481 aggaaacgcc aaaggtttag ccttccaagc cattcaacaa gctaaagatg gaaaatttgc
      541 agaagcagaa agctcattaa atgaagcgag tgaacaactt cgtgaagccc atgatgttca
      601 aacagatctc ttaacacgtt tggcacaagg agaaaaaatc ggctggaatc tttacatggt
      661 tcacgctcaa gaccatttga tgaatgctat tacctttaaa gaccttgcag tagaagttgt
      721 tggtcaagaa cgacgccttc aagcacttga aaacaaataa tacagctcct agcttattag
      781 aaaagcaaga ttaaaagttg cggagacgca acttttttct aacaaaaatc gttgagtaag
      841 aaaggagtag aaaatgagtt tttttggaaa tgttgacttt caaaaattaa cctatacaga
      901 aaggacttgc tacagttatc ttcgcgataa tgttgataaa atcccttatt tacgcgtccg
      961 tgacattgct ttagaggccc atgttggaac ttcaagcgtc atgcgtttaa tacataaaat
     1021 gggttatgat tcgtatacgg attttaaaga atatattatt gataaaaaag agctagaaaa
     1081 aggaatttct aacactgcta ttcctttctc atcagatatt ttttcaggag acgttgaaca
     1141 gcgtttagat aatttagcgc aacgtgtgat tgaaagtgat aatatcattt ttaccggagt
     1201 tggctcaagt ggtttaatct gtgattatgc agctagaaga ttagctggtg tgggcattaa
     1261 tactttttca tttagcgatg taacttatcc gattgcttca aaattgcaaa atacaaccaa
     1321 tactttagta attgctttat ccatttcagg tgaaactaat gaaatcattg aagtactgac
     1381 gagtcttcgg tcaaataaag atgtttatat ttcgagtatc acgcccaaaa ttaattcgag
     1441 tatcgctgaa ctgagcgatt ttgtcctgac ctatcgaatc aatgaacacc gaattaacac
     1501 gcattatgac ttaacgaggc aattacctac ggtctatttg acagaacgtt taacagatct
     1561 cgtttatcaa cgttccaact aaaaaagcca atctggcttt tttctatgct ctgtcgttcc
     1621 taatggtttg atatctaaaa gtaaaaaagt taaattgaga taacaaatat aattatcaag
     1681 gctgaacctc gcaagcttaa aggaaactta tatgacaatt ttggtacagg agtcttcaaa
     1741 agtggcacag aaccaaagtg atggaaaaat aagaaaccgc tttctttact ttgcctatta
     1801 atgctataat gaaaatgtag aaaagatgga cgtgaaacca gttcatcaaa aaaagtaaag
     1861 gagactgttc aaccatgaac aattttattc aaaacaaaat catgcctcca atgatgaaat
     1921 ttttgaatac ccgtgcagtc acggcaatca aaaatggtat gatttatcct atcccattta
     1981 tcattattgg ttcagtattc ttgattcttg gtcaactgcc attccaagca ggacaagact
     2041 tcatgaacaa aatcaaattg ggcccactct ttttacaaat taataatgct tcatttggta
     2101 ttatggcttt gcttgccgtg ttcggtattg cttacgcttg ggttcgagat gcaggttatg
     2161 aaggagtacc cgctggttta acaggtgtca ttgttcacat cttgttgcaa ccagacacaa
     2221 tccatcaagt aacaagtgtt actgacccaa ctaaaacatc aacagcattt caagtaggtg
     2281 gtgtcattga ccgagcttgg ttaggtggga aagggatggt tctctcaatc atcgttggac
     2341 tcttagtagg ttggatttac actggcttta tgcgtcggaa catcacaatc aaaatgccag
     2401 aacaagttcc agaaaacgtt gccgcatcat ttacttcact tgtacctgca ggagcaatca
     2461 ttacaatggc tggtgtggtt catggaatca caacgattgg cttcaacaca actttcattg
     2521 agttagttta taaatggatt caaacaccat tgcaacacgt gactgacggt ccggttgggg
     2581 tcttcgttat tgcctttatg ccagtattta tctggtggtt cggtgttcac ggagcgacaa
     2641 tcattggtgg gattatggga ccattgcttc aagcaaactc tgctgacaat gctgctctct
     2701 acaaagcagg acatcttagc ctgtcaaatg gcgcccatat cgttactcaa tcatttatgg
     2761 accaatactt gacagttact ggttctggtt tgaccattgg tttggttatc ttcctcttag
     2821 tgagtgcaaa atcagttcaa ggtaaaactt taggacgaat ggaaattgga cctgcagtat
     2881 tcaatatcaa cgaacctatt ctgtttggac ttcctatcgt tttgaatcca attcttgcta
     2941 ttccatttat cttggctccg ttgatttcag gaattttgac ttacttagtg atttatctag
     3001 gaatcattcc accatttaat ggtgcctatg ttccttggac aacccctgcg gtcttgtcag
     3061 gatatctagt aggtggctgg caaggtatgg tttggcaaat tattattctt gctttgacca
     3121 cagttctcta ttggccattt gccaaagctt atgacaatat tcttctgaaa gaagaagctg
     3181 aaacagaagc tggaattaat gctgccgaat aattgcaaaa tttagatgag ctcaagactt
     3241 cttgagctta tcttgttaag aaatgataat ttaataattc taataattac aaggagaaat
     3301 tatgacttta aaaaaagact tcctctgggg aggagcagtt gctgcacatc aacttgaagg
     3361 aggctggaat gctggtggaa agggagtttc cgtagcagat gtgatgacag caggtagtaa
     3421 cggcgtagaa agaaaaatca cagatggagt aattgaggga gaaaattacc ctaatcatga
     3481 agctattgat ttctatcacc actataaaga agatgtagca ctttttgcag aactcggttt
     3541 aaattgtttt agaacatcta ttgcttggac acgcattttt cctaagggag atgagtctga
     3601 acctaacgaa gagggcttgc aattctatga tgacttattt gatgaatgtt taaaaaatgg
     3661 tattgaacct gtcattactc tttctcactt tgaacttcct tatcatttag tgacagagta
     3721 tggtggattc cgaaaccgaa aactcattga tttctttgtc catttttcag aagtagtgat
     3781 gaatcgttat aaagataaag ttaagtattg gatgactttc aatgaaatta ataaccaagc
     3841 aaactttatg cgcgactttg ccccttttac caattctggt ttgaaatttc cagagggagc
     3901 aagcgaaaaa gagcgtgaag aaatcatgta tcaagcggct cattatgaac ttgttgcctc
     3961 ggccaaagtt gtagagttgg gacataaaat taatcctgat ttccaaattg gttgtatgat
     4021 tgccatgtgt ccgatttatc ctgcaacttg taaaccggaa gacatgatgg cttcaaccgt
     4081 tgctatgcaa cgtcgttatt ggtttgcaga tgtccacgtt cgcggacatt acccaagtta
     4141 tttaaaggct tattttgaac gtaaagaatt taaacttgat attagtgatg aagacttaga
     4201 agttttgaaa aatggaaaag ttgattatat tggtttttct tactatatga gttttgcaat
     4261 taaggaccat ggaaaagccc caacttttga ttataacgaa gataaagatt tagttaaaaa
     4321 tgactatgtt aaggcttctg aatgggggtg gcaaattgac ccattaggtt tacgttatgc
     4381 gatgaattgg ttctatgaac gttatgaaaa accacttttc attgttgaaa atggctttgg
     4441 tgctgtagat gaagttgaag ctgatggaag tattcacgac ccatatcgaa ttgaatattt
     4501 aaaagctcat atagaatcaa tgaaagaagc tgttgaatat gatggggttg atttaatggg
     4561 atatactcct tggggcttta ttgaccttgt ttctgctgga accggtgaga tgaaaaaacg
     4621 ctatggtgtc atctatgtgg acaaagataa tgaaggaaat ggaacattaa aacgctctaa
     4681 aaaagattca tttgattggt atcagaaagt tattgcaacc aatggtgaag aactttaa
//
