LOCUS       NZ_CP020030            11118 bp    DNA     linear   CON 20-DEC-2019
DEFINITION  Geobacillus thermodenitrificans strain T12 chromosome, complete
            genome.
ACCESSION   NZ_CP020030
VERSION     NZ_CP020030.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06459234
            Assembly: GCF_002119625.1
KEYWORDS    RefSeq.
SOURCE      Geobacillus thermodenitrificans
  ORGANISM  Geobacillus thermodenitrificans
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Daas,M.J.A., Vriesendorp,B., van de Weijer,A.H.P., van der Oost,J.
            and van Kranenburg,R.
  TITLE     Complete genome sequence of Geobacillus thermodenitrificans T12, a
            potential host for biotechnological applications
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11118)
  AUTHORS   Daas,M.J.A., Vriesendorp,B., van de Weijer,A.H.P., van der Oost,J.
            and van Kranenburg,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2017) Laboratory of Microbiology, Wageningen
            University & Research, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.0.4
            Genome Coverage                   :: 164.0
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/19/2019 16:15:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 3,737
            CDSs (total)                      :: 3,609
            Genes (coding)                    :: 3,470
            CDSs (with protein)               :: 3,470
            Genes (RNA)                       :: 128
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 75 of 139
            Pseudo Genes (incomplete)         :: 88 of 139
            Pseudo Genes (internal stop)      :: 31 of 139
            Pseudo Genes (multiple problems)  :: 45 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            CP020030.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Geobacillus thermodenitrificans"
                     /mol_type="genomic DNA"
                     /strain="T12"
                     /isolation_source="soil"
                     /db_xref="taxon:33940"
                     /country="Netherlands:ede"
                     /lat_lon="52.0439 N 5.6167 E"
                     /collection_date="22-Oct-2012"
     gene            complement(1..831)
                     /gene="kduI"
                     /locus_tag="GTHT12_RS10165"
                     /old_locus_tag="GTHT12_01416"
     CDS             complement(1..831)
                     /gene="kduI"
                     /locus_tag="GTHT12_RS10165"
                     /old_locus_tag="GTHT12_01416"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="WP_060475675.1"
                     /translation="MEIRYAHHPEDIKTYTTEQLRSQFLIERVFVEDEVCFTYTHVDRM
                     IFGGVMPKNKELTLPSGKTLGVAYFLERREMGVINVGGQGSIVIDGEEYEMNKRDGLYV
                     GKGAKEVIFRSNDPNAPAKFYVTSAPAHQTYPTVKISIEQIHPLRLGETNQLNKRAIYQ
                     YIHPNTCQSCQLQLGLTMLEPGSAWNTMPCHTHERRMEVYFYFDMAPETRVFHFMGKPD
                     ETRHLVISNEQAVISPSWSIHSGVGTSNYTFIWAMCGENISYDDMDHVKMEELR"
     gene            complement(865..1620)
                     /gene="kduD"
                     /locus_tag="GTHT12_RS10170"
                     /old_locus_tag="GTHT12_01417"
     CDS             complement(865..1620)
                     /gene="kduD"
                     /locus_tag="GTHT12_RS10170"
                     /old_locus_tag="GTHT12_01417"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="WP_060475677.1"
                     /translation="MTTNFSLHGKIAIVTGASRGLGQGMAIGLAEAGADIIGVGVSNME
                     GTKTEIEKLGRKFYGISLDLSQPDAAYRLVEDVFRIVDRVDILVNNAGIIRREQAENFS
                     DKDWEEVTSINLDSVFKITREIGKCMIKQGKGKIINIASMLSFQGGIKVPAYTASKHAV
                     AGLTKAFANEWASKGINVNAIAPGYMITDNTAAIRADKERNASISARIPAGRWGTPEDL
                     KGPVVFLASDASNYVNGHILCVDGGWMSY"
     gene            complement(1751..2512)
                     /locus_tag="GTHT12_RS10175"
                     /old_locus_tag="GTHT12_01418"
     CDS             complement(1751..2512)
                     /locus_tag="GTHT12_RS10175"
                     /old_locus_tag="GTHT12_01418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_DeoR,DBD-SUPERFAMILY|0043758"
                     /protein_id="WP_060475676.1"
                     /translation="MLSVKRYEKILEELNKRGAVKVAELSKQFSVTEKTIRADLEYLEK
                     RGYLKRVHGGAVLPEGDIDLFPIHKRQEGHSEEKMAIARTALATIQPNETILMDGGSTT
                     LALARLLGDFPVTVITNDVKIAYSLIEKDKVHLLVLGGMRVGSSSSLISSESLEKLKSI
                     HVDRLFLGTTAVDVKSGLSVFNSLHADWKKRCIQCANRVTLLADSTKFGKVALLQFASI
                     EDVDEIITDRMIDQAVLEQLGQFDLRVILPT"
     gene            complement(2888..4099)
                     /locus_tag="GTHT12_RS10180"
                     /old_locus_tag="GTHT12_01419"
     CDS             complement(2888..4099)
                     /locus_tag="GTHT12_RS10180"
                     /old_locus_tag="GTHT12_01419"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.0,HMM:NF024242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0036286,DBD-SUPERFAMILY|0035607"
                     /protein_id="WP_087960078.1"
                     /translation="MYKILLVDDDRLIRRSLATTIPWKRYGYELVGEASNGEEGLSLID
                     QHKPQIVISDIKMPFMDGLQMASIGKTRYPEMKVILLTGYEDFKFAQEAIKIKAFDYLL
                     KPVESSILLKKVQQAAMEWEYEQRSQMQKVKGIRFLKQKWLKKLLHQRHHSLEIYNEME
                     KFNVYLKGECVAVSIIRLEDIPKEGQLDEERLDHAYKICSQAIENQGEVFDWEENEFIV
                     ILSGHSKEEILKEMKKLIEQICLDIEAELGIRATAAAGEVHNTTSIALSYFQAREVLKS
                     QRVVAQKQIGYRPHEDYGSVVDKAIEYMEQHYNKEGLTLNEVAQAVHVNPSYLSNMFKV
                     KKGTNFSDCLLELRMRKAMELLRDRNAKVYEVAEMVGYSNPQYFSVCFKKYVGLSPLDF
                     KKSG"
     gene            complement(4123..5919)
                     /locus_tag="GTHT12_RS10185"
                     /old_locus_tag="GTHT12_01420"
     CDS             complement(4123..5919)
                     /locus_tag="GTHT12_RS10185"
                     /old_locus_tag="GTHT12_01420"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014567.0,HMM:NF018305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="WP_060475608.1"
                     /translation="MKEPVGRKGKALESIKRYLFSSFQSTILLLYLPIIILCLALTGWI
                     SYHLSSKQIEENAYQNVRDTVFQTKNYLDNRLHDIFEQLVSLSNNSSILSIMSRDPFYT
                     EPDDYIQVNKQISQIYSTYNSLLDSVFVNFHNGDFVLLKCDVSPKKIHFSYEEYRGRFK
                     GNREGYYWRNLHYDDVLNSNEVVSVFKLIGQNTSSTHGILLFNLRKDFFEKVLSKSLLG
                     ENGYLLLISPDGQMLSKQVDAKYQLTSEWIDHLRNIEEKSGRMEFKKHDKETMIVVYDT
                     LSINNWKVVAVFSEGDMLKNVNYIKYTTVVVIISLIIAASLLAKVFSEYAARPISLLAR
                     DMKRFYKDRLGMPADISTLREIHILQKGIQELMQHIDNLMEQIKKEQEKKKKLEFAVMH
                     AQIHPHFLYNTLYSIKSLCDMGMNREASSMISALSNFFRISISKGQEMISIEEEIEHIK
                     SYLFIQEMRYGDDFSYEIDVDQHILSFRIIKLTLQPLVENAIYHGVKQKRGKGTILVKG
                     YCINDTIYFEVIDNGVGMDVNKIKEIMDALNNKEENAALGIGLRSVHERIQLHYGSSYG
                     MHIESELGKGTKVTVTVPAEKV"
     gene            complement(6234..7559)
                     /locus_tag="GTHT12_RS10190"
                     /old_locus_tag="GTHT12_01421"
     CDS             complement(6234..7559)
                     /locus_tag="GTHT12_RS10190"
                     /old_locus_tag="GTHT12_01421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009328690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL1_6|PL1"
                     /protein_id="WP_087960079.1"
                     /translation="MRRWYTNIFLVVLLACLSIPMQANAKELGHEVLKPYDGWAAYGEG
                     TTGGAMASPQNVFVVTNRTELIQALGGNNHTNQYNSVPKIIYVKGTIDLNVDDNNQPVG
                     PDFYKDPHFDFEAYLREYDPATWGKKEVEGPLEEARVRSQKKQKDRIMVYVGSNTSIIG
                     VGKDAKIKGGGFLIKNVDNVIIRNIEFEAPLDYFPEWDPTDGTLGEWNSEYDSISIEGS
                     SHIWIDHNTFTDGDHPDRSLGTYFGRPFQQHDGALDIKNSSDFITISYNVFTNHDKVTL
                     IGASDSRMADSGHLRVTLHHNYYKNVTQRLPRVRFGQVHIYNNYYEFSNLADYDFQYAW
                     GVGVFSQIYAQNNYFSFDWDIDPSLIIKVWSKNEESMYETGTIVDLPNGRRYIDLVASY
                     NESNTLQLKKEVTWKPMFYHVIHPTPSVPALVKAKAGAGNLH"
     gene            complement(7556..8686)
                     /locus_tag="GTHT12_RS10195"
                     /old_locus_tag="GTHT12_01422"
     CDS             complement(7556..8686)
                     /locus_tag="GTHT12_RS10195"
                     /old_locus_tag="GTHT12_01422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006636120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH105"
                     /protein_id="WP_060475610.1"
                     /translation="MAGQVKSYSPLYLAEQACQSLMRTYSPQELPPAGKWHYHQGVFLY
                     GMLKVWEKTGKQEYFDYVKGYFDYLIDEEGNFVFARDELDSLQPGMLLFTLYKETGDRR
                     YKIAAKKLRGLFSTLNRTREGGFWHKDKYPYQMWLDGLFMAGPFALMYSYEYHEPELID
                     MVLFQEKLMRTHMKDAKTGLLYHAWDEKRETPWSHPVTGCSPEFWGRSIGWYCAAVVEI
                     LDRLPQNHIERRELMESIQNLVKSLIDFQDQETGLWYQVVDKGDREDNWLESSCSSLFI
                     YAIAKAIQKEYIDSKYLQAAQFAYDGLLKHMIAYDTNGNWVMKGICIGTSAGKYDYYVS
                     RATSENDLHGVGAFVLASTALQEVIENEGKAGGVLE"
     gene            complement(8927..10249)
                     /locus_tag="GTHT12_RS10200"
                     /old_locus_tag="GTHT12_01423"
     CDS             complement(8927..10249)
                     /locus_tag="GTHT12_RS10200"
                     /old_locus_tag="GTHT12_01423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="WP_087960377.1"
                     /translation="MCVISLVALLVLSGCNNSEKTAVKDDANKSASSKEQIVLTFWDEN
                     AGPQRTPIWQELIKRFEEANPNIKVEYVGLPKDMAKQKYDAAISANDTPDVGSIQTSWL
                     SEFSIREALVPLDDYFDKWSEKEKINKGAIEFNRNTVIDGKLYGIPYTQNIDILWYRPD
                     WFKQAGVKPPETWDEFFNAIEKMTDKSQKRYGFTIRGGDGASIQLQRMMYAYSGIEQYF
                     DENGKSTINHPKHVEFVKKYLGLYKKYTPESDITNGYKEMIAAFDTGVAALVQHNIGSY
                     GEHSKALKPDQFAALPLPKTDDGKYMVEGGNTINISIFKNTKHPEAAWKFVSFLTSAES
                     QSYWNQQTGQVPTHADVMNEQWVKDAQHIKVAFNVLENKDTVFYNPAFYLPDYRSILDN
                     LVDPGIQSVMSGKKSVEDFLNEWASALEKSKQQYDKQFSKK"
     gene            complement(10294..11118)
                     /locus_tag="GTHT12_RS10205"
                     /old_locus_tag="GTHT12_01424"
     CDS             complement(10294..11118)
                     /locus_tag="GTHT12_RS10205"
                     /old_locus_tag="GTHT12_01424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|G4FGN6|3.A.1.1.41"
                     /protein_id="WP_060475618.1"
                     /translation="MTKLDRFITFHMPLTLMVVFTIFPFYWTLNIAFKREGDILQRPLE
                     YIPSSPTIENFLVAWNNVGFATYFKNSLFVAVCTVLLVLVLATLSGYALSRYQFRGKRA
                     FLLLLLSTQFVPRAMLIIPLFIIFKHLGLISNPLSLIIAYTTVELPFTSILMNGFISSV
                     PKELEEAAMIDGCNRLQSIRYVVFPLLLPGIVATGVFTFIYVWNEFLLALMLTNQQSRF
                     TLPVGLSYMLGEFNINYGALAAGSVIALLPAILLFMYAQRHLVSGLGGAVKG"
ORIGIN
        1 ttatcttaat tcttccatct tgacatgatc catatcatcg tatgaaatat tctcgccgca
       61 catagcccaa ataaatgtat agttgctcgt tccaacccct gaatggatgg accagcttgg
      121 agaaatcacg gcttgttcgt tgctgatcac aagatgcctt gtctcatccg gcttacccat
      181 aaagtgaaat acccgagttt ctggagccat atcaaagtaa aagtacactt ccattcgccg
      241 ttcatgagtg tgacatggca tcgtattcca cgcactgcct ggctctagca tcgtcaaccc
      301 taactgtagt tggcaacttt ggcaagtatt tggatgaata tactgatata tggctcgttt
      361 atttaactga ttggtttcac ctaaacgaag cgggtgaatt tgttcaatcg aaatttttac
      421 agttggatat gtttgatgag ccggcgcaga tgttacataa aatttggcag gggcattagg
      481 atcgttggag cgaaagatca cttccttcgc accttttcca acgtataaac catcccgttt
      541 gttcatctca tactcttcgc catcgatcac aatgctgcct tgtcctccaa cattaataac
      601 gcccatttca cgccgctcta aaaaatatgc tactcccaac gtttttcctg acggcaaagt
      661 caattctttg tttttaggca tgacaccgcc aaaaatcata cgatcgacat gagtgtaagt
      721 gaaacaaact tcatcttcca cgaatactct ttcaatcaaa aattgactgc gtaactgttc
      781 tgttgtatac gttttgatat cctcggggtg atgtgcataa cgtatttcca tcagcatttc
      841 ctcccgccca ttgtatgtaa tgttttaata gctcatccat ccaccatcaa cacataaaat
      901 atgtccattt acataattag aagcatctga agccaaaaac acaaccggtc cttttaaatc
      961 ttccggagtc ccccaccgtc ccgctggtat ccgcgcagaa atggatgcat tccgctcttt
     1021 atcagcccga atggctgctg tgttatccgt gatcatataa ccaggagcaa tggcattaac
     1081 attgattccc ttgcttgccc attcattggc gaatgctttt gtcaagccag ctactgcatg
     1141 tttgctagct gtataagccg gcactttgat gccgccttgg aaagacaaca tagaggcaat
     1201 attaataatt tttccttttc cttgtttgat catgcacttg ccaatttctc ttgtgatttt
     1261 aaatacactg tctaaattga tgctagtgac ttcttcccaa tctttatcag aaaaattttc
     1321 agcctgttct ctccgaatga tccccgcatt gttaaccagg atatcgactc gatcaacaat
     1381 cctaaacaca tcctcaacta aacggtatgc cgcatctggt tgggaaagat cgagagaaat
     1441 tccataaaac ttacgtccta atttctcaat ttccgttttt gtcccttcca tattgctgac
     1501 tcctacaccg ataatatcgg cgcctgcttc agctaaacct atcgccattc cttgccctaa
     1561 tcctctactt gcaccagtga caatcgcgat ttttccatgc aacgaaaaat tggttgtcat
     1621 ttcgtgcagc acccccattc ccgtttatgt acgttaatgt tcgtttgtgt tctcttttct
     1681 ccgattatat catcccaaaa aagaaacgtc aatgaatttt ctatccatac ataatgttca
     1741 gagtattttg ttatgtgggt aaaatgactc gtaaatcgaa ttgtcctagt tgttctaaaa
     1801 cagcttgatc aatcattcgg tccgtaatga tttcgtccac gtcttcgatg gacgcaaact
     1861 gaagcaaagc tacttttccg aactttgtag aatcagccaa taatgtcacc cgattggcgc
     1921 attgaatgca cctttttttc caatcggcat gcaagctgtt gaaaacactt aatccgcttt
     1981 tgacatcgac ggctgttgtt cctaaaaata gccgatccac atgaatggac tttaactttt
     2041 ccaatgattc cgaactaatt aatgaggaag aactccccac tctcatccca cccaaaacaa
     2101 gcaaatgtac tttatccttt tcaattagac tataggcgat ttttacatca ttcgtaataa
     2161 ctgtcacagg gaaatctccc aataatcgcg ccagtgccaa tgtagtgcta ccgccgtcca
     2221 tcaaaatcgt ttcatttggc tgtatggtag caagcgccgt ccgagcgata gccatttttt
     2281 cttcactatg tccttcttgc ctcttgtgaa ttggaaataa atctatatcc ccctcaggaa
     2341 gaacagctcc accgtggacc ctttttaaat accctctttt ttcaaggtat tccaaatcag
     2401 ccctaatcgt cttctccgtt accgaaaatt gcttgcttaa ctccgcaact tttacagccc
     2461 cccgtttgtt tagttcctcc agtatttttt cgtaacgttt cactgaaagc ataccgtctc
     2521 ctccgacaca ataaataata tggtccgcac acagattgag aaaacaagag aagatatgga
     2581 gaggcaacat ttttcgattg gcatcctaac cataaacacc taaaatatta ccacaaaaac
     2641 aaacattttc aaaccttttc gaacaaccat aacgaaaaac acctcaagaa acaaacaagc
     2701 ttctaagcat gattgtcccc cctacttttt accagtaacg gatctgtatc tcaccattgc
     2761 ccttatccct tattcttccc ttacatttca cgacttctaa catcctatgt gaagtcttgc
     2821 ggaacgagag gcataaatga gcaaaaaaaa aatatttcct taaaacatga gttaagaaac
     2881 aacgccttta tcctgatttt ttgaaatcca acggagaaag acctacatac tttttaaagc
     2941 aaacgctaaa atattgcgga ttgctgtatc ccaccatttc cgcaacctca tacacctttg
     3001 cattcctgtc ccttaatagt tccattgctt ttctcatcct tagctccaaa agacaatcag
     3061 agaaattcgt cccttttttc accttaaaca tgttactgag gtaagaagga ttcacatgga
     3121 cggcttgagc tacttcattt aaagtaagcc cttctttgtt ataatgttgt tccatgtatt
     3181 caattgcttt atccaccaca gacccatagt cttcatgcgg tctatatcca atctgttttt
     3241 gtgcaacaac acgctgggat ttcaaaacct ctctcgcctg aaaatacgac agagctatcg
     3301 atgtagtgtt atgcacttcc cccgctgcag ctgtagctct gatccctagc tctgcctcaa
     3361 tgtccaagca tatctgttcg attaactttt tcatctcttt taagatttcc tctttagaat
     3421 gacctgaaag aataacaata aactcgtttt cctcccaatc gaatacttca ccttggtttt
     3481 ctatcgcctg gctgcatatt ttataagcat gatctaaacg ttcttcgtct agttgccctt
     3541 cctttggaat atcctctaaa cgaatgatgg agacagcgac acactctcct tttaaataaa
     3601 cattgaattt ctccatttca ttatatattt ccaatgaatg atgacgttga tgaagcaatt
     3661 tctttaacca tttttgcttc agaaatcgaa tcccctttac tttttgcatt tgactccttt
     3721 gttcatattc ccattccatt gccgcctgtt ggactttttt caataatata gaactttcaa
     3781 ccggcttgag caaataatca aaagccttta tttttatcgc ttcttgcgca aacttaaaat
     3841 cttcataacc tgtcagtaat atcactttca tctctggata acgtgtcttt cctatactcg
     3901 ccatctgcaa tccatccata aaaggcattt taatatcgga aatcacaatc tggggcttat
     3961 gctgatcaat caatgataat ccctcttccc cgttactggc ctcgccaacc aactcatatc
     4021 catatctctt ccatggaata gtggtggcta agcttcttct aatcaggcga tcgtcatcca
     4081 caagaagaat cttatacatt ctcgacacct ctgctcccct ttttatactt tttctgccgg
     4141 aacagttaca gtgactttcg tacctttccc aagttcactt tcaatatgca ttccataaga
     4201 tgagccgtaa tgaagttgaa ttcgctcgtg aacgctgcga agaccgatcc ctagcgcagc
     4261 attttcttct ttattattta aagcgtccat aatctctttt attttattta cgtccattcc
     4321 cactccatta tcaatcacct caaaataaat ggtatcgttg atacaatatc cttttactaa
     4381 gatggttccc ttgccccttt tttgtttcac cccatggtaa attgcatttt ccaccaatgg
     4441 ctgcaaggtc aacttgataa ttcgaaatga taaaatatgt tgatctacat cgatctcata
     4501 ggaaaaatca tctccatacc gcatttcttg aatgaacaaa taacttttta tatgctcgat
     4561 ttcctcctct atgctaatca tttcttgccc tttgcttatg ctgatcctga aaaagttaga
     4621 aagcgcgctg atcattgaac tcgcttctcg attcataccc atatcgcata agctttttat
     4681 cgaataaaga gtgttatata aaaaatgagg atgaatctgg gcatgcatca ccgcaaactc
     4741 taattttttc ttcttctctt gttccttttt tatctgctcc attaagttgt caatatgctg
     4801 cattaattct tgaataccct tctgcaaaat atggatctct cttaaggtag aaatatccgc
     4861 tggcatacct aatcgatcct tataaaatct cttcatatca cgcgctaaca aagaaatggg
     4921 acgagccgca tattcagaaa aaactttcgc taataaggat gcggcgataa taagactaat
     4981 aatcaccaca acggtggtat atttaatgta atttacattt tttaacatat caccctcaga
     5041 aaagacggca accactttcc aattattgat cgataatgta tcataaacaa cgatcattgt
     5101 ttctttgtca tgttttttga attccattct cccgcttttt tcttctatgt tcctcaagtg
     5161 atcaatccac tcgctggtta attggtattt ggcatccacc tgcttagata acatctgtcc
     5221 atccggacta atcaaaagaa ggtatccatt ctctccaaga agcgacttgc tcaacacttt
     5281 ttcaaaaaaa tctttacgta gattgaataa aaggatgcca tgagtggaag aagtgttttg
     5341 gccaatcaac ttaaatacac tgactacctc attactgttt aaaacatcat cgtaatgaag
     5401 atttctccaa tagtatccct ccctgtttcc tttaaaacgt cccctatatt cttcatatga
     5461 aaaatgaatc ttttttggag aaacatcaca ttttaaaagc acaaaatcgc cgttatggaa
     5521 attgacaaat acagagtcca atagcgagtt atacgtagaa tagatttggc tgatttgttt
     5581 attcacttga atgtaatcat cgggttctgt ataaaaagga tctcttgaca taatcgataa
     5641 aatggaagaa ttattcgata aggagaccaa ttgttcgaat atatcatgca gacgattatc
     5701 taaatagttt ttcgtttgaa aaactgtatc tcttacattt tggtatgcat tttcctcaat
     5761 ttgcttagaa gatagatgat aagagatcca cccagtgagt gccaaacaca aaatgataat
     5821 tggcagatat aacaaaagga tggtggactg aaatgaagaa aataaatacc ttttgatgct
     5881 ctccaacgct tttcccttcc tgccgaccgg ctctttcatt acaattattt cctcacttcc
     5941 cctcttattt aaacgctttc attttcttgc aaataagaaa aataagcggc tcttgcactc
     6001 cttttggcaa gatcaaagag gcaatcatca atgcaacact gagaagttct cgtttttcct
     6061 actgtccaat cgagagcctc ttttcaccaa aatgtgcaag aaccacaaaa agtaaatata
     6121 agataataat aaggtattcc aatttttgtt gacaatccct cattgatgat cccgagaaaa
     6181 aagatcatag cccaaagatg agcattttac atataccgca tagaattcgg agactaatga
     6241 agatttcccg cacccgcttt tgctttgact aaagctggca cacttggagt agggtgtata
     6301 acatgataaa acatcggctt ccacgtgact tcctttttca gttgcaaagt gtttgattca
     6361 ttataggatg cgactaagtc aatgtatcgc cgtccattag gaagatcaac tattgttcca
     6421 gtttcataca tcgattcttc atttttgctc cagactttta taatcaaact aggatcgata
     6481 tcccaatcaa aggaaaaata attattttga gcataaatct gagaaaaaac acctactccc
     6541 caagcatatt gaaagtcata atcagcaaga ttcgagaatt cataataatt attgtatata
     6601 tgaacttgac caaatcgaac gcgcggcaaa cgctgtgtga catttttata ataattgtgg
     6661 tgcagtgtca ctcgcaaatg tccactatcg gccattcggc tgtcacttgc tccgattagc
     6721 gtcaccttat catggttagt aaatacatta taggaaatag tgataaaatc ggaactattt
     6781 ttgatatcca gtgctccatc atgctgctga aacggtctcc caaagtatgt gcctaaagaa
     6841 cgatcaggat gatcgccgtc agtaaaagta ttatgatcga tccatatatg cgaagatcct
     6901 tcaatggaaa tgctatcata ttcagaattc cattcaccta gtgttccatc agttggatcc
     6961 cattcgggga aatagtcaag aggagcttca aattcaatat tgcgaatgat tacgttatcg
     7021 acatttttta ttaaaaaacc tccccctttg atttttgcgt ctttccctac gccaataata
     7081 gatgtgttag agccaacata aaccatgatt cgatcttttt gttttttctg cgaacgtacc
     7141 cgtgcctcct ctaacggacc ttcgacttct tttttccccc acgttgcagg gtcatattcc
     7201 cgcaagtatg cttcaaaatc aaaatgtgga tccttataaa aatccggtcc tacaggttga
     7261 ttattatcgt ctacattcag gtctatcgtg ccttttacat aaatgatttt aggaacacta
     7321 ttgtattggt ttgtatgatt atttcctcct aacgcttgaa tgagttccgt cctatttgtc
     7381 acaacgaaaa cgttttgcgg actggccatc gcccctcccg ttgttccttc tccatacgct
     7441 gcccatccat catagggttt cagtacttcg tgccctaatt ccttggcatt ggcctgcata
     7501 gggatagata aacatgctaa aaggacgacc aagaatatat tcgtatacca tcgtctcatt
     7561 ctagaacacc ccctgccttt ccttcatttt caataacttc ttgcaaagcg gtgctggcta
     7621 acacaaacgc ccctacccca tgtaaatcat tctctgaagt ggcacgtgac acataataat
     7681 catatttccc tgctgaagtg ccaatacaaa tccctttcat gacccagtta ccattcgtgt
     7741 cataagcaat catgtgtttc aataatccgt cataagcaaa ttgagctgct tgcaggtatt
     7801 ttgaatctat atactctttt tggatcgcct tggcgatggc atagatgaat agacttgagc
     7861 atgaagattc cagccagtta tcctcccggt ctcccttatc taccacttga taccataatc
     7921 ccgtttcctg atcttgaaaa tcgatgagtg acttgaccaa gttctgtatg gattccatta
     7981 attctcttct ctctatatga ttttgtggca atcggtcgag tatttccaca acagcagcgc
     8041 aataccatcc aatcgatctt ccccaaaatt caggcgaaca acctgtcact ggatgggacc
     8101 aaggggtttc tcttttttca tcccaagcat gatataataa ccctgttttg gcatctttca
     8161 tgtgagtacg catcaatttt tcttgaaata acaccatatc aatcaattcc ggttcatgat
     8221 attcataact gtacattaaa gcgaatggcc ctgccatgaa caatccgtca agccacatct
     8281 gatagggata tttatcttta tgccaaaatc cgccttctct tgttcgattc aatgtcgaaa
     8341 ataacccccg cagcttcttg gcagcaattt tatatctacg atcccccgtt tctttatata
     8401 gagtgaacag caacatccca ggctgcaacg agtcgagttc atctctagca aagacaaaat
     8461 tcccctcttc atcaatcaaa taatcaaaat atcccttcac ataatcaaaa tattcctgtt
     8521 tgcctgtctt ctcccagacc tttagcattc catacaaaaa taccccctga tggtaatgcc
     8581 acttgccggc aggaggcaat tcctgtggtg aatacgttct catcaaagat tggcatgcct
     8641 gttccgctaa gtacaatgga gaatacgatt ttacttgacc ggccatagaa cgatccctcc
     8701 tagaccaaag ctttttcacg tcttttctga aagacaattt ccgtatcaag gaatgggcag
     8761 agtgatcgcc caatgagtaa gcggaaaatg aatgtcgaat agcatcagcc aaaacccatg
     8821 tctagcaaac gggatatttc atacaaagga cacagaggag aaatctattc ccctatacca
     8881 gtgtaagatg tgcggatgct aaaaagcatc cgcccttatc atccttttat tttttactga
     8941 attgcttatc atattgctgt ttcgattttt ccaaagcact tgcccactcg ttcaagaaat
     9001 cttcaactga ttttttaccg ctcatcacac tttgaatgcc tgggtcaaca agattatcta
     9061 agattgagcg ataatctgga agatagaaag ctggattgta gaaaacagta tctttatttt
     9121 ccaacacatt aaaagcgact ttaatatgct gagcatcttt cacccattgt tcgttcatca
     9181 catctgcatg tgtaggaact tgtcctgttt gttgattcca ataactttgg ctttcagcgg
     9241 aagtcaaaaa gctaacaaat ttccaagcag cttcaggatg tttagtattt ttaaagatac
     9301 taatattaat agtgtttccg ccttcaacca tatattttcc atcatctgtt tttggcaacg
     9361 gcaatgcggc aaactgatcc ggctttaatg ccttgctatg ctcaccgtac gaaccgatgt
     9421 tatgttgtac gagcgccgcc acccctgtat caaatgctgc aatcatttct ttgtacccgt
     9481 ttgtaatatc gctttctgga gtatatttct tatacaatcc taaatacttt ttgacaaatt
     9541 caacatgttt aggatggtta atcgtacttt ttccattctc atcaaaatat tgttcaattc
     9601 ccgaatatgc atacatcatc cgttgcaact ggatagaggc gccatctcca ccgcgaatcg
     9661 tgaatccgta gcgtttttgg cttttatctg tcattttttc gatcgcattg aaaaattcat
     9721 cccaagtttc cggtggtttt acaccagctt gtttaaacca atctggccga taccaaagaa
     9781 tatcgatgtt ttgcgtatat ggaatcccat ataattttcc gtcgatcaca gtattacggt
     9841 taaactcgat tgccccttta ttgatttttt ctttttcact ccacttgtca aaataatcat
     9901 ccaacggcac taaagcttcg cgaatcgaaa attcggacaa ccagctcgtt tgaatacttc
     9961 cgacatccgg cgtatcatta gcagaaatgg ccgcatcata tttttgtttt gccatatctt
    10021 tcggaaggcc cacgtactct actttaatat tcggatttgc ttcttcaaac cgcttgatca
    10081 gttcttgcca aattggcgtt ctttgcggcc cggcattctc atcccagaat gtcaatacga
    10141 tttgctcttt ggatgatgca gacttgtttg catcatcttt gacggctgtt ttttcactat
    10201 tattgcatcc gctaaggacc aataatgcca ctaagctgat tacacacaca aaactccaaa
    10261 cttttttata cacgttctat ccccccatct tttttatccc tttacagcac cgcccaagcc
    10321 gcttacgaga tgtctttgag catacatgaa tagaagaatg gctggcaata aagcaattac
    10381 acttccagct gctagcgcac cgtagttaat gttgaattct cctaacatgt agctcaaacc
    10441 gacaggaaga gtaaagcgac tttgttggtt agtaagcatt aacgccaata aaaattcatt
    10501 ccaaacgtag ataaacgtaa agactcctgt tgctacaata cctggcaata gtaaaggaaa
    10561 aacgacataa cgaatagatt ggagacggtt acatccgtcg atcatagcag cttcttccag
    10621 ttccttcggt acgctggaaa taaatccatt catcagaatt gatgtaaaag gaagctcaac
    10681 agttgtataa gcaataatga gcgaaagtgg attgctaatt aatccaagat gtttgaaaat
    10741 aataaataac gggataatca gcatagccct aggaacgaat tgcgtactga gcaacagaag
    10801 aagaaatgct cgtttccctc taaactgata tctgcttagt gcataaccag ataatgtggc
    10861 caaaacaaga accaataata cagtacaaac agcaacaaac aagctgtttt taaaataagt
    10921 agcaaacccc acattattcc aagccactag gaagttctca atcgtaggtg aactaggtat
    10981 atattccagt gggcgttgaa gaatatctcc ttcacgttta aaagcaatat tcaatgtcca
    11041 gtaaaatggg aaaatcgtga acacaaccat tagtgtcaac ggcatatgga acgtaataaa
    11101 acgatctaac ttcgtcat
//
