LOCUS       KF840174               13632 bp    DNA     linear   BCT 16-MAR-2014
DEFINITION  Geobacillus stearothermophilus strain T-1 carbohydrate utilization
            gene cluster, complete sequence.
ACCESSION   KF840174
VERSION     KF840174.1
KEYWORDS    .
SOURCE      Geobacillus stearothermophilus
  ORGANISM  Geobacillus stearothermophilus
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Zehavi,A., Shulami,S., Lavid,N. and Shoham,Y.
  TITLE     Carbohydrate utilization gene cluster from Geobacillus
            stearothermophilus T-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13632)
  AUTHORS   Zehavi,A., Shulami,S., Lavid,N. and Shoham,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2013) Department of Biotechnology and Food
            Engineering, Technion-Israel Institute of Technology, Haifa 3200,
            Israel
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: MAQ v. 0.7.1; VELVET v. 0.7.26; EDENA v.
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            2.1.1; DNAdiff v. 3.20
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Geobacillus stearothermophilus"
                     /mol_type="genomic DNA"
                     /strain="T-1"
                     /db_xref="taxon:1422"
     misc_feature    1..13632
                     /note="carbohydrate utilization gene cluster"
     gene            1..1437
                     /gene="gan1D"
     CDS             1..1437
                     /gene="gan1D"
                     /EC_number="3.2.1.85"
                     /note="glycoside hydrolase family 1"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1"
                     /protein_id="AHL67640.1"
                     /translation="MEHRHLKPFPPEFLWGAASAAYQVEGAWNEDGKGLSVWDVFAKQP
                     GRTFKGTNGDVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGNGAVNEKGLDFY
                     DRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVK
                     YWVTLNEQNIFISFGYRLGLHPPGVKDMKRMYEANHIANLANAKVIQSFRHYVPDGKIG
                     PSFAYSPMYPYDSRPENVLAFENAEEFQNHWWMDVYAWGMYPQAAWNYLESQGLEPTVA
                     PGDWELLQAAKPDFMGVNYYQTTTVEHNPPDGVGEGVMNTTGKKGTSTSSGIPGLFKTV
                     RNPHVDTTNWDWAIDPVGLRIGLRRIANRYQLPILITENGLGEFDTLEPGDIVNDDYRI
                     DYLRRHVQEIQRAITDGVDVLGYCAWSFTDLLSWLNGYQKRYGFVYVNRDDESEKDLRR
                     IKKKSFYWYQRVIETNGAEL"
     gene            complement(1443..2198)
                     /gene="ganR2"
     CDS             complement(1443..2198)
                     /gene="ganR2"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|HTH_6,STP|SIS"
                     /protein_id="AHL67641.1"
                     /translation="MFTPEQIAQFSELDYAIYDYVMKHPHEVAYMRIRELAEAVHVSPP
                     TVLRFCKKVGCEGFSEFKTKWKLYLRETKRTAIISSQEVLKEFFERTLTTDYERTILDA
                     AAVIARADHVIFVGSGSSGILAEYGSRYFSAFQKFSVYIKDPFFPIYGHYFHNCAVIAL
                     SVSGETSYTLAQVHRFKENGSTIISITNRKHCTLANISDYNLTYYTTPERIGQTDVTTQ
                     LPVVYLLERLAKEMYRLLPAQTDRGPSGR"
     gene            2323..3666
                     /gene="gan4C"
     CDS             2323..3666
                     /gene="gan4C"
                     /EC_number="3.2.1.86"
                     /note="glycoside hydrolase family 4"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 6-phospho-beta-glucosidase"
                     /protein_id="AHL67642.1"
                     /translation="MGKRLKMATIGGGSSYTPELVEGLIKRYHELPVGELWLVDIPEGK
                     EKLEIVGALAKRMVEKAGVPIEIHLTLDRRRALEGADFVTTQFRVGGLEARAKDERIPL
                     KYGVIGQETNGPGGLFKGLRTIPVILDIIRDMEELCPDAWLINFTNPAGMVTEAVLRYT
                     KQEKVVGLCNVPIGMRMGVAKLLGVDADRVHIDFAGLNHMVFGLHVYLDGVEVTEKVID
                     LVAHPDRSGVTMKNIVDLGWEPDFLKGLKVLPCPYHRYYYQTDKMLAEELEAAKTKGTR
                     AEVVQQLEKELFELYKDPNLAIKPPQLEQRGGAYYSDAACSLISSIYNDKRDIQPVNTR
                     NNGAIASIPPESAVEVNCVITKDGPKPIAVGDLPVAVRGLVQQIKSFERVAAEAAVTGD
                     YQTALVAMTINPLVPSDTIAKQMLDEMLEAHKEHLPQFFKQAKTVAGN"
     CDS             3880..4746
                     /codon_start=1
                     /transl_table=11
                     /product="putative transposase"
                     /protein_id="AHL67643.1"
                     /translation="MSETRIDHDRLFKELLSTFFEEFLLLFFPHVYEQVDFRHVSFLSE
                     EVFTDVAAGEKHRVDLLVETKLKGEDGLIIVHIEHQSYTQPTFPERMFIYVSRLFQKYR
                     RRILPIAIFSYDEHRDEPSSFTIKFPFLTVVDFRFLTVELRKQPWREYIRRDNPVAAAL
                     LSKMGYNESERVAVKREFLRMLVRLELDEAKQRLLFGFFETYLTLSEQEEIQLRNEVNQ
                     MGTKEAKKVTDLIVSYEQRGMEKGIEKGIEKGKMDIAKRMLGKGYDVPTICELTGLSVE
                     AVEKLKG"
     gene            4966..5979
                     /gene="ganP"
     CDS             4966..5979
                     /gene="ganP"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|G4FGN5|3.A.1.2.20"
                     /protein_id="AHL67644.1"
                     /translation="MRDWWKRLFYGGGIAALIGSASFTAYCAWNVYAYPKSEKERVPET
                     TKEAYHFVLVPEELDNDYWRLVEKGAKAAAKELGVDLEYIGPRQANIDEHLRILKKAAA
                     AKVDGIITQGLTEAEFVPVINEITDKNIPVVTIDTDAPTSRRVAYVGTDNYYAGFLAGR
                     ALAEDTKGKATVAIITGSLTAAHQQLRVRGFEDAVRQEKGIRIVAIEESHITRVQAAEK
                     AYTILKKHPDVNAFYGTSALDAIGVAKVVEQFHREQKTYIIGFDTLPETIRYLQKGTIA
                     ATVVQEPYEMGYKAVKMMAEIVAGKDVPVVTNTETKVIRKKDLPLRPARNYEVNTP"
     gene            6142..7611
                     /gene="ganS"
     CDS             6142..7611
                     /gene="ganS"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="AHL67645.1"
                     /translation="MNIRTKMLLCFTVFLLLLNGAVFLLYQTSEEMMSDYDQRMRRLLL
                     LNEVSQRANRMMEQLNAYVSEKEGRYARAYEREFRWLQQRRRQLGAILPVLSDRLAAEN
                     YEHMIESLLEEAALTVYHFQAGNIGLYSSHLHETMNIASFLQEETLNLIDDELTAYQRR
                     YDEVERRNRYFRYMGMGLFVTTLLLGALLAVFFSGHLTKPIILLSRAARSIADGRLDGP
                     DIEPMTNDELRLLTITFNDMRRNLKQLIAEMKQKAELDRLVKELELKSLQSQINPHFLF
                     NTLNTVAKMAYLEDAQQTSRLIEAVAAILRYNLGDLQRTVTLADEVRIAREYFFIQQTR
                     FFDRIKFSLEAEPSCLDQPIPPLTLQPLIENAFIHGIETYEQGAELSVSVFAENGRVVV
                     EVRDNGVGMDEQVKAELEALIRGEEPRTRREQGRGHSTGIGLHNVIRRLQLFYGVMDVA
                     EIESALGKGTTVRLWLPRWQGGMNGESGDRR"
     gene            7589..9130
                     /gene="ganT"
     CDS             7589..9130
                     /gene="ganT"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|Response_reg,STP|HTH_18,STP|HTH_AraC"
                     /protein_id="AHL67646.1"
                     /translation="MKVAIVDDEALERRALCKMISDHLPDIEVVAEGANGREAVEIAKR
                     YRPDIMLIDIKMPGLDGLQAIEAIRQDGLDLEFIIVSAFDLFDYAKQAMRFGVKEYLLK
                     PSRKEEVISALERVSQEVAAKRRHEESSRQLEEQIRRLQTLVESEWLSVLMTEDVSADE
                     WERWKELLPFSIASGMFLVIQFPDAGVADEWKSWLDKQLSGKAPTRYWIGRMANRRLPV
                     LFFRSPNDGEPAWKPVIQALALDLARQFSARYGAALYIGLGSPFSRLDQLRSSYYEALS
                     AAHYYADRQKAQVGFLPAEATRAGGEAERDKQLFEALRLGDIEQARMICLTYIEELASS
                     HSLPAAGRKAEETFVWLGRLLSELGIRYERLASFASCRSAAELKRAALDELDRIAADLE
                     VWRQQQAHGKLGKAKDYIDRHYAEPLTLEEVAEQAGISPYYFSKLFKEQFGITFIDYVT
                     NVRIERAKEALAETDQSLKEICFSVGYNDPNYFSRVFKKQTGLSPSEYRKKVQAR"
     gene            9445..10743
                     /gene="ganE2"
     CDS             9445..10743
                     /gene="ganE2"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q72KX2|3.A.1.1.24"
                     /protein_id="AHL67647.1"
                     /translation="MRKKASAWLALALGVGMALSGCSSSNSSSSDDAKQGSQKAGEKLE
                     IFSWWTGAGEEDGLKALIKLFQEKYPDIEVENAAVAGGAGTNAKAVLASRMQGNDPPST
                     FQVHGGAELNEGWVAAGKMEPLNDLYEKEGWMDKFPKALIDMVSKDGNIYSVPVNIHRG
                     NVLWYNKKVFADNGLQPPKTFDEFFQVADKLKAKGITPLALGDKEPWAATHLFENVLLG
                     TLGTENYKKLWTGELSFNDPQVKQAVETFKKMLGYINEDHSSRNWQDAAQLVAEGKAAM
                     YVMGDWVKGYFVNDLKLKVNQDFGYVPVPNTEGKFMVITDTFGLPKGVKNPDDVKKFLS
                     VLGSVEGQDAFNPLKGSIPARIDADPSKYDEYGKQTMQDFKTAELAPSLAHGSAAPEGF
                     VTKVNQAVNIFVTQKDVKTFIDTLASAAAELKK"
     gene            10805..11776
                     /gene="ganF2"
     CDS             10805..11776
                     /gene="ganF2"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q72KX3|3.A.1.1.24"
                     /protein_id="AHL67648.1"
                     /translation="METVNITPAEKTAAVPAVRKKRKWTADHWLAAAFLAPSVVLIFLF
                     VYGFIGWTGYVSLSNWNSLVPDWSFAGLKNYLYLFHDFRFQADLRNTLVFTVLFIAAVI
                     VLGQLLAILLDQKLRGESLFRNIFFFPMALSFVVTGVVWQWLFNPSTGVNLFLKPLGLD
                     GKWYTDTNILFGFHWGKIEFGIPVAIIAVVVAAVWQMTGFAVAMYLAGLRAIPDEVREA
                     ARMDGATEFQLYWKIILPLLRPITVSVIIIMAHISLKIFDLIYAMTGPGANFVTDVPGV
                     YMFETTFRGNYYANGAAIAIVMLLSVAIFIVPYLISSRKGGS"
     gene            11776..12597
                     /gene="ganG2"
     CDS             11776..12597
                     /gene="ganG2"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q72KX4|3.A.1.1.24"
                     /protein_id="AHL67649.1"
                     /translation="MARSMWARPFLYLLALVMSVFFLLPVYVMVTTSLKPLDEVTLADM
                     WKLPSTIDWSSYATAFDKLAPNFWNSILLVVPATLLSALLGSLNGYVLSKWKFKGADTL
                     FTLILFGMFIPYQSILIPLIQFLREIGLYNTIPGLVFVHVVYGIPITTLMFRNFYAAIP
                     DEMIESAKIDGAGFVRIFRYIMLPLSITGFVVVAIWQFTNIWNEFLFAVTITTSDKQPI
                     MVALQNLSGSQIVQWNVQMAGALLAALPTLLVYIFLGKYFVRGLLAGSVKG"
     gene            12760..13632
                     /gene="frk"
     CDS             12760..13632
                     /gene="frk"
                     /codon_start=1
                     /transl_table=11
                     /product="putative fructokinase"
                     /protein_id="AHL67650.1"
                     /translation="MILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDF
                     FRPHRIEAIGIGSFGPVDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTD
                     VNAAALGELRWGAAQGLDSCLYMTVGTGIGVGAVVEGRLLHGMLHPEMGHMFVRRHPDD
                     AFAGICPYHGDCLEGMASGPAIERRWGKKGAELANRSEVWELEAFYLAQAIANYILILS
                     PKKVITGGGVMKQTHVLPLVRRHVQELLNGYVQHETILEKIDEYIVLPGLGDNAGVAGA
                     LALAAAANE"
ORIGIN
        1 atggagcatc gtcatcttaa accgttcccg cctgagtttt tatggggcgc agcatcagct
       61 gcgtatcaag tcgaaggggc gtggaacgaa gatggaaaag gattatcggt atgggatgtg
      121 tttgccaaac agccgggccg aacgtttaag ggaaccaacg gcgacgtcgc tgtcgatcat
      181 tatcatcgct atcaagaaga tgtagcgttg atggcggaaa tggggttgaa agcgtatcgg
      241 ttttcggtgt cgtggagccg cgtgtttccg gatggaaacg gggctgtcaa cgaaaaaggg
      301 cttgattttt acgatcgctt gattgaggag ctgcgaaacc atgggatcga gccgattgtg
      361 acgttatacc attgggatgt gccgcaagcc ttgatggatg cctatggggc atgggaatca
      421 cggcgcatca ttgatgattt tgaccggtat gccgtgacat tgtttcaacg gtttggcgac
      481 cgggtcaaat attgggtgac actcaatgaa caaaacattt tcatttcttt tggctatcgt
      541 cttggcttgc atccgccggg cgtaaaagac atgaaacgca tgtatgaggc aaaccatatc
      601 gccaatttgg cgaatgcgaa agtgattcaa tcgttccgtc attacgtgcc tgacggaaaa
      661 atcgggccga gctttgccta ctcgccaatg tacccgtacg acagccgtcc ggaaaatgtg
      721 ctcgcttttg aaaacgcaga agaatttcaa aaccattggt ggatggatgt gtatgcgtgg
      781 ggcatgtacc cacaggcggc ttggaactat cttgaatcgc aagggctgga gccgacggtg
      841 gcgccgggcg attgggagct gctccaagcg gcgaaaccgg actttatggg agtcaactat
      901 taccaaacta cgactgtgga gcataatccg ccagatggcg tcggagaagg tgtgatgaat
      961 acgacgggaa aaaaagggac atccacttcg agcggcattc cgggactgtt taaaaccgtg
     1021 cgcaatccgc atgtcgatac gacgaactgg gattgggcga ttgatccggt tggcttgcgt
     1081 attggcctgc gccgcattgc gaaccgttac cagctgccga ttttgattac agaaaacggc
     1141 ttaggtgagt tcgatacgct ggagccaggc gatatcgtga atgacgatta tcgaatcgac
     1201 tacttgcgcc gccatgttca agaaattcaa cgcgccatca cggatggggt ggacgtgctc
     1261 ggttactgtg cttggtcgtt caccgatttg ctcagctggc tgaatggcta ccaaaaacgg
     1321 tacggatttg tgtacgtcaa ccgtgacgat gaatcggaaa aagatttgcg ccgcatcaaa
     1381 aagaaaagct tttattggta tcagcgtgtg attgagacga acggtgccga gctgtagcct
     1441 tgtcaccgcc cgctcggccc tcggtcagtc tgcgccggca gcagccgata catttccttc
     1501 gccagccgct caagcaagta gacgaccgga agctgagtgg tgacatccgt ctgtccgatc
     1561 cgctccggcg tggtatagta tgtcaagttg tagtccgaga tgttcgcgag tgtgcaatgt
     1621 ttccgattgg tgatgctgat gatcgtgctg ccgttttcct tgaagcggtg cacttgggcg
     1681 agcgtatagg acgtctctcc agacaccgac aaggcgatca cggcgcagtt gtggaaataa
     1741 tggccgtaaa tcggaaaaaa cggatccttg atgtagacgg aaaacttttg aaacgcggaa
     1801 aaataacgcg agccgtattc ggccaaaatg cccgagctgc ccgatccgac gaaaatgacg
     1861 tgatccgccc gggcgatcac agctgccgca tccagaatgg tccgctcgta atcggtcgtc
     1921 agcgtccgtt cgaaaaattc cttaagcacc tcttgactgc tgatgatcgc ggttcgcttt
     1981 gtctcgcgta aatacagctt ccatttcgtt ttaaattcac tgaacccttc gcagccgacc
     2041 tttttgcaaa agcggagcac agtcggcggc gagacatgga cggcttcggc gagctcgcgg
     2101 atgcgcatgt acgcgacttc atgcggatgt ttcatcacat agtcgtaaat ggcgtagtcg
     2161 agttcggaga actgagcgat ttgttcggga gtgaacatcg ggatacctcg ctttcatggg
     2221 acagaacatg cgatagccgg gaaaggatgg cgctttcaag cccactggtt gattcctaca
     2281 ttgattatac catgcccaac tgggcgaaag gggaagagga gtatggggaa acgattgaaa
     2341 atggcgacga tcggcggcgg gtcaagctac acgccggaat tggtcgaagg gttgatcaag
     2401 cgttatcatg aattgccggt cggggaactg tggcttgtcg atattccgga aggcaaagaa
     2461 aaacttgaaa tcgtcggcgc ccttgccaaa cggatggtcg aaaaagccgg tgtgccgatc
     2521 gagattcatt tgactttgga tcgccgtcgg gcgctagaag gagctgattt tgtcacgacg
     2581 cagtttcgcg tcggcggtct tgaggcgcgg gcgaaagacg agcgcatccc gctcaaatac
     2641 ggggtgatcg gccaggagac aaacggcccg ggcggattgt ttaagggatt gcggacgatt
     2701 ccggtcattt tggacatcat tcgcgatatg gaagagcttt gccccgatgc atggctgatc
     2761 aacttcacaa acccggctgg aatggtgacg gaagccgtct tgcgctacac gaagcaggaa
     2821 aaagtcgtcg gtttgtgcaa cgtgccgatc ggcatgcgca tgggcgtcgc caagctgctt
     2881 ggcgttgatg ccgaccgcgt gcacattgat tttgccggct tgaaccatat ggtgtttggc
     2941 ttgcacgtct atctggacgg cgtcgaggtg accgaaaagg tgattgatct cgtcgcccat
     3001 ccagaccgct ccggtgtgac gatgaaaaac atcgttgacc ttggctggga gccggatttc
     3061 ttaaaagggt tgaaggtgct gccttgcccg taccaccggt attactacca aacagacaaa
     3121 atgctcgccg aggagctgga ggcggcgaaa acgaaaggga cgcgcgccga agtcgtccaa
     3181 cagctcgaaa aagagctgtt tgagctgtac aaagacccga acttggccat caagccgccg
     3241 cagctcgaac agcgcggcgg ggcgtactac agcgacgccg cctgcagctt gatcagctcc
     3301 atctacaacg acaagcgcga cattcagccg gtcaacacga gaaacaacgg cgccattgcc
     3361 agcattccgc cagaatcagc cgttgaggtc aactgcgtca tcacaaaaga tgggccgaaa
     3421 ccgatcgccg tcggcgactt gccggtcgcg gtgcgcgggc tcgtccagca aatcaagtcg
     3481 ttcgagcgcg tcgcagccga agcggcggtc accggcgact accaaacggc gctcgtcgcc
     3541 atgacgatca acccgcttgt cccgtctgac accatcgcca aacaaatgtt ggacgaaatg
     3601 cttgaagcgc ataaagaaca tttgccgcag ttttttaaac aggccaaaac agtagctgga
     3661 aactaaatcg tttggaaaag cgattgacaa agccgcctgt cgccggggcg gctttgtcat
     3721 ttcatcgtga aaagccgcac aacacaaggg ctttcttccc gttcacgttc cccttatttt
     3781 agacaaaatc aatgcttttc accagccttt tcggcaggat tttgcctaag gcgcggcgaa
     3841 gaaaataaag gaagttcaaa tatcacaagg agggagaccg tgtccgaaac gcgaatcgat
     3901 cacgaccggt tgtttaagga gttgctgtcg acgttttttg aagagttttt gcttctgttt
     3961 tttccgcacg tgtatgaaca agtcgacttc cgccacgtct cctttttgtc cgaagaggtg
     4021 tttacggatg tggcggccgg cgaaaagcac cgtgtggatc tattggtgga aacaaaattg
     4081 aaaggggaag atgggctgat catcgtccat atcgaacacc aaagttacac gcagccgacg
     4141 tttcccgagc gcatgttcat ttatgtcagc cgtttgtttc aaaaataccg ccgtcgcatt
     4201 cttcccattg ccatcttcag ctatgacgaa caccgcgatg aaccgtcctc gttcaccatc
     4261 aagtttccgt tcctaaccgt tgtcgatttc cgctttctta ctgttgaact gcgcaaacag
     4321 ccatggcgtg agtacattcg ccgcgacaac ccggtcgcgg ccgccttgtt aagcaaaatg
     4381 gggtataatg aaagtgagag agtggcagtg aaaagagaat ttttgcgcat gctcgtccgc
     4441 ttggagcttg acgaagcgaa acagcggctg ttgtttggat ttttcgagac gtatttgacg
     4501 ctgtccgaac aagaggaaat ccaattgcga aacgaggtga accaaatggg aacgaaggaa
     4561 gcgaaaaaag tgacggatct catcgtgtcg tatgagcaac gggggatgga aaagggaatc
     4621 gaaaaaggaa tcgaaaaagg caagatggac atcgcgaaac ggatgctagg gaaaggatat
     4681 gatgtgccaa cgatttgtga attgactgga ctgtcggttg aggcagtgga aaagttgaaa
     4741 ggataaacga gtggagagac cccgttccga tgcagcgagc ttgtttcgga acggggtttt
     4801 tttgctcgct ctgcacttga tgatgggcat ggccatcgcc gtatggcgaa tgatgccggc
     4861 cggactgacc cctatttgga tttcatatag atgcaattca tccattttct tttgtctagg
     4921 ctagcgcccc gacgttcgtg tacaatggcg gtaggaggga tcaagatgcg cgattggtgg
     4981 aaacggctgt tttacggggg cggcatcgcc gcgctgattg gaagcgcttc cttcaccgcc
     5041 tattgcgctt ggaacgtgta tgcgtatccg aaatcagaaa aggaacgggt tccggaaaca
     5101 acaaaagagg cctatcattt cgtgcttgtg ccggaggaac tcgataatga ttactggcgg
     5161 ctcgtggaaa aaggagcgaa agcggcggcg aaagaactgg gggttgacct cgagtatatc
     5221 ggcccgcggc aggcgaatat tgatgagcat ttgcgcattt tgaaaaaagc cgcggcagcg
     5281 aaagtcgatg ggatcatcac ccaagggttg acggaagcgg aatttgtccc agtgattaac
     5341 gaaatcacgg acaagaatat tcctgtggtg acgatcgata cggatgctcc aacgagccga
     5401 cgcgtcgcct atgtgggaac ggataattat tatgccggtt ttctcgcggg acgggcgttg
     5461 gctgaggata caaagggcaa ggcgacggtc gcgattatta ccggcagttt gacggcggcg
     5521 caccaacagc tgcgagtgcg cggatttgag gatgcagtga gacaggaaaa aggaatccgc
     5581 atcgttgcca tcgaagagtc gcacattacg cgtgtgcaag ccgcggaaaa ggcgtatacg
     5641 attttgaaaa agcacccgga tgtgaatgcg ttttacggca cgagcgcgct tgatgcgatt
     5701 ggcgttgcca aggtggtcga gcagtttcat cgggagcaaa agacgtatat tattggcttt
     5761 gacacgctgc cggagacgat ccgctatttg caaaaaggaa cgattgcggc gacggtggtg
     5821 caggagccgt atgaaatggg atacaaggct gtcaaaatga tggccgagat cgtcgccggg
     5881 aaagacgtgc cggtggtgac gaatacggag acgaaagtca ttcggaaaaa agacttgccg
     5941 ctgcgcccgg cgcgcaatta tgaggtcaac acgccatgaa ggggcctgcc aggcctccgt
     6001 agggaggggg ctgaaagcgg agaagggttg gcgagcgcta aagcatgaga gatggccgtc
     6061 cttgacgttc gaacacgttc gacttgaagc ggcaggacga tgcaggcaac aaagcgccgg
     6121 tgacagcggg gtggaattca gatgaacatt cggacgaaga tgctgctttg ctttaccgtt
     6181 tttttgcttt tgctgaacgg agcggtgttt cttttgtatc agacgagcga agagatgatg
     6241 agcgattatg atcaacggat gcgccgattg ttgttgctca atgaggtgtc gcagcgggca
     6301 aaccgaatga tggaacagct caatgcgtac gtctcggaaa aagaaggacg gtatgcacgc
     6361 gcttatgagc gggagttccg ctggctgcag caacgccgcc ggcagctcgg cgccatcttg
     6421 ccagtgctgt ccgaccggct ggcggcggaa aactatgaac atatgattga aagtttgctg
     6481 gaggaggcgg cgctgacggt ctatcatttt caagctggaa atattggctt gtactcatcc
     6541 cacttgcatg aaacgatgaa cattgcgtcc tttttgcaag aggaaacgtt aaacttgatt
     6601 gacgatgagc tgacggccta ccaacggcgg tacgatgaag tggagcggcg caaccgctat
     6661 ttccgctata tgggaatggg attgtttgtc acgacgttgt tgcttggcgc cttgctggcg
     6721 gtgttctttt ccggccactt aacgaagccg atcattcttc tctcgcgcgc tgcccgatcc
     6781 atcgctgacg ggcggctgga cgggccggat attgagccga tgaccaacga tgagctgcgg
     6841 ctgttgacga tcacgtttaa tgacatgcgc cgcaatttaa agcagctgat tgcggagatg
     6901 aaacaaaagg ctgagctcga ccggctggtg aaggagctgg aactcaaaag cttgcaaagc
     6961 caaatcaatc cgcatttttt attcaatacg ctgaacacgg ttgcgaagat ggcgtacttg
     7021 gaggacgccc agcaaacgtc gcggctgatt gaagcggtgg cggccatttt gcgctacaac
     7081 ttgggtgatt tgcagcggac ggtgacgctt gccgatgagg tgcgcatcgc ccgtgaatat
     7141 ttcttcatcc agcagacgcg gttttttgat cggatcaagt tttcgttaga ggcggaacca
     7201 tcttgcctcg atcaaccgat tccgccgctc acgttgcagc cgctgattga aaatgcgttc
     7261 atccatggca ttgagacgta cgagcaggga gccgagctgt ccgtgtccgt tttcgccgaa
     7321 aacgggcggg tcgttgtgga agtgcgcgac aatggcgttg gcatggatga gcaggtgaaa
     7381 gcggagcttg aagcgttgat ccgcggcgaa gaaccgcgga cgcgccgtga gcaagggcgc
     7441 ggccattcaa ccggcatcgg cttgcacaat gtcattcgcc gactgcagct gttttacgga
     7501 gtgatggatg tggccgaaat cgagtctgca ctaggaaaag gaacgacggt gcggttatgg
     7561 ctgccaagat ggcaaggggg gatgaacggt gaaagtggcg atcgtcgatg atgaggcgtt
     7621 ggagcgaaga gcgctttgca aaatgattag cgaccacctt ccggatatcg aggtggtcgc
     7681 cgagggcgcc aacgggcgcg aggcggtcga gatcgccaag cgttatcgtc cggatatcat
     7741 gctgattgac atcaaaatgc ccggccttga cgggctgcaa gcgatcgaag cgattcgcca
     7801 agacggcctc gatctggagt tcattattgt gtcggcgttt gatttgttcg actatgcgaa
     7861 acaagcgatg cggtttggcg tcaaggaata tttattgaaa ccgagccgga aggaggaggt
     7921 catttcggct ttggaacggg tcagccaaga agtggcggcc aagcggcgac acgaggagag
     7981 cagccgccag ctcgaggagc agattcgccg gttgcagacg cttgtggaaa gcgaatggct
     8041 gtccgtcctc atgacagaag acgtatcggc tgatgagtgg gagcgatgga aggaactgct
     8101 gccgttttcg attgcgtcgg ggatgttcct cgtcattcag ttcccggatg caggggtggc
     8161 tgacgaatgg aaatcatggc tggacaagca gcttagcggg aaggcgccaa cgcgctattg
     8221 gatcgggcgg atggcgaacc ggcgcctgcc ggtcttgttt ttccgcagcc caaacgatgg
     8281 cgagccagcc tggaagcccg tcatccaggc attggcgctc gatttggcgc ggcagttttc
     8341 ggcccggtac ggcgccgcgc tgtatatcgg gctcggctcc ccgttttccc gccttgacca
     8401 acttcgttcc tcgtactatg aggcgctgtc ggccgctcat tattacgccg accggcaaaa
     8461 agcacaagtg gggtttctgc cggcggaagc gacgcgcgcc ggcggggaag cggaacggga
     8521 taaacagctg tttgaagcgc tgcgccttgg cgacatcgag caagcccgga tgatttgtct
     8581 gacgtatata gaggaactgg cctcttctca ttcactgccg gccgccggcc gcaaagcgga
     8641 agagaccttt gtgtggctcg ggcgtcttct atcggagctg ggcattcgtt atgagcggct
     8701 cgcttccttt gcttcctgcc ggtcggcggc agaattgaag cgagcggcgc ttgatgaact
     8761 ggaccgcatc gccgctgatc tcgaggtttg gcgccagcag caagctcacg gcaaactcgg
     8821 caaggcgaaa gactacattg accgtcacta cgccgagccg ctgacgcttg aggaggtggc
     8881 ggaacaagcg ggcatcagtc cgtactactt cagcaaactg ttcaaagagc agtttggcat
     8941 cacctttatc gactacgtga cgaacgtgcg catcgaacgg gcgaaagaag cgctggctga
     9001 gacggatcaa agcttaaaag aaatttgttt ttcagtcggc tacaacgacc ccaactattt
     9061 cagccgcgtc tttaaaaagc aaaccggcct gtcgccgagc gaataccgga aaaaagtaca
     9121 ggcgcgctga caaggggagt gctgtctcca acaaggaagg tgcatggcaa gtcaaaaggt
     9181 aagtcccgcg gacaaagcag gctccgccct tcttccggcg tccgaagcga agcccgcccc
     9241 cctgatgggg agacgggctt ttttcgcgcc ataaaagcga aatggcagtc cgctgcacac
     9301 cgcttgcctg acaggagaac ggaaaacaaa aaagtgaaga aaaacgcaaa aaagtgaaga
     9361 aatcaagccg ccgattgata agcggcccat ggtacactca acattgaaag ggatttcaaa
     9421 aacaaacgga aaagggggag gaacatgaga aagaaagcat ccgcatggct ggcgttggcg
     9481 cttggcgtcg gtatggcgct gtcgggctgc agcagctcga actcttcttc gtccgatgac
     9541 gccaaacaag gcagccaaaa agcgggagag aagctcgaga ttttcagctg gtggaccgga
     9601 gccggtgagg aagacggctt aaaagcgctc atcaaactgt tccaagaaaa atatccggat
     9661 attgaagtcg aaaacgcggc ggtggccggc ggggcgggaa cgaacgccaa ggcggtgttg
     9721 gcgagccgca tgcaaggaaa cgatccgcca tcgacgttcc aagtacacgg cggagcggag
     9781 ctgaatgaag gctgggtggc cgccggcaaa atggagccgc tcaacgattt atatgaaaaa
     9841 gaaggctgga tggacaaatt cccgaaagcg ttgattgaca tggtgagcaa agacggcaac
     9901 atttactccg tgcctgtcaa cattcaccgc ggcaacgtgc tttggtacaa caaaaaagta
     9961 tttgccgaca acgggcttca gccgccgaaa acattcgacg agtttttcca agtggccgac
    10021 aagctgaaag caaagggcat tacgccgctc gccttaggcg acaaagagcc gtgggcagcg
    10081 acgcacctgt ttgaaaacgt gctgcttggc acgctcggaa cggaaaacta caagaaactt
    10141 tggacgggag aattatcgtt taacgaccca caagtgaaac aagccgttga gacgtttaag
    10201 aaaatgcttg gctatattaa cgaagaccat agctcgcgca actggcaaga cgccgcccag
    10261 ctcgtcgccg aaggaaaggc ggccatgtac gtgatgggcg actgggtgaa gggctatttt
    10321 gtcaacgatt tgaaattgaa ggtgaaccaa gacttcggct atgtgccggt gccgaatacg
    10381 gaaggcaagt ttatggtcat taccgatacg ttcggcctgc cgaaaggcgt gaaaaacccg
    10441 gacgatgtga agaagttctt atcggtgctt ggttcggtgg aagggcaaga tgcgtttaac
    10501 ccgctgaaag gctccatccc ggcccgcatc gacgcggatc cgtccaagta cgatgaatac
    10561 ggcaaacaaa cgatgcaaga cttcaagacg gcggagctgg cgccgagctt agcgcacggt
    10621 tcagcagcgc cggaagggtt tgtcacgaag gtcaatcagg ccgtcaacat tttcgtgacg
    10681 caaaaagatg tgaagacgtt catcgacacg ttggcatcgg ccgccgcaga actgaagaag
    10741 taatcgggaa aaggggacgg gagccattct cttccccttt tcgtcatcat gaggagggat
    10801 cttagtggaa acggtcaaca tcacgccagc cgagaagaca gcggctgtcc ccgcggtgcg
    10861 gaaaaagcgg aaatggacgg ccgatcattg gctggcagcg gcgtttttgg caccgtcggt
    10921 cgtactgatt ttcttatttg tgtacgggtt tatcggctgg acggggtatg tgtccttgag
    10981 caactggaat tcgctcgtgc cggattggtc gtttgccggg ttgaaaaact atctgtattt
    11041 gtttcatgat ttccgcttcc aggccgactt gcgcaataca cttgtgttta cggtgttgtt
    11101 tatcgccgcc gtcattgtgc tcggccaact gcttgccatt ttgcttgacc aaaaactgcg
    11161 cggcgagtcg ctgttccgca acattttctt cttcccgatg gccttgtcat ttgtcgtcac
    11221 cggagtcgtg tggcaatggc tgttcaaccc gtcaacgggc gtcaacttgt ttttaaaacc
    11281 gcttggcttg gatggaaaat ggtatacgga tacgaacatt ctgttcggat ttcattgggg
    11341 caagattgag tttggcatcc cggtcgccat cattgcggtg gtcgtggcgg ctgtttggca
    11401 aatgactggc tttgccgtcg cgatgtatct cgccggcctg cgcgccattc cagatgaagt
    11461 gcgggaagcg gcgcggatgg acggagcgac cgagtttcaa ttgtattgga aaatcatttt
    11521 gccgctgctg cggccgatca ctgtcagcgt catcatcatc atggctcata tttcgctcaa
    11581 aattttcgat ttgatttacg ccatgaccgg gccgggggcg aactttgtca ccgatgtgcc
    11641 gggcgtgtac atgtttgaaa cgacgttccg cggcaactat tacgccaacg gcgcggcgat
    11701 tgcgatcgtg atgctgctgt cggtcgccat ctttatcgtt ccatatctca tctcaagccg
    11761 caagggggga tcgtgatggc gagatccatg tgggcgcggc cgtttttgta tttgttggcc
    11821 ctcgtcatga gcgtgttttt cctgttgccg gtgtatgtca tggtcacaac gagcttgaag
    11881 ccgctcgatg aagtgacatt ggccgatatg tggaagctgc cgtcaacgat cgattggagc
    11941 agctacgcga cggcatttga caagctcgcc ccaaactttt ggaattcgat tttgctcgtc
    12001 gtgccggcga cgctgctctc cgcgctgctg gggtcgctca acggctatgt actgtcgaaa
    12061 tggaagttta aaggagcgga tacgctcttt acgctcattt tgttcgggat gttcattccg
    12121 tatcaaagca ttctcattcc gctcatccaa tttttgcgcg aaatcgggct gtacaacacg
    12181 attccggggc ttgtgttcgt ccatgtcgtc tacggcattc cgatcaccac actgatgttt
    12241 cggaatttct acgcggcgat ccccgatgaa atgattgagt cggcgaaaat cgacggcgct
    12301 gggtttgtcc gtatttttcg ttatattatg ctccccttgt ccatcactgg atttgtcgtc
    12361 gtggcgatct ggcagtttac aaacatttgg aacgagtttt tgttcgccgt gacgatcacg
    12421 acatcggaca aacagccgat catggtcgcc ttgcaaaact tatccggcag tcaaatcgtc
    12481 caatggaacg tgcaaatggc gggagccctg ttggcggcgc tgccgacctt gcttgtgtac
    12541 atcttccttg gcaaatattt tgtccgtggg ttgttggctg gttcggtgaa ggggtgagcc
    12601 aatcagcagt gtagatcaac aaacccggta gacaatagaa tcgattgcgc tatgtttcaa
    12661 aaagccgctg atcagcggct ttttgtcatt gatgccctct ccctttttct agtaagatag
    12721 atacaagaaa agagcagatg aagaaagaga ggcgtacata tgatcctagg agccattgaa
    12781 gctggaggca cgaaatttgt ctgcgccatc ggcgatgaac aggggaacat ccatgaacga
    12841 gcggtgtttc cgacgacggc gccggaagag acgatggcgc acgtcatcga ctttttccgt
    12901 ccgcaccgca tcgaggcgat tggcatcggg tcgttcggcc cagtcgattt gcgtccggac
    12961 agtccgacgt acgggtacat tacgagcacg ccgaaacagg cgtgggcgca gtttgatttt
    13021 gtcggcacga tgaagcagca ttttcccgtg ccgatcggct ttgacacgga tgtcaacgcg
    13081 gcggcgctcg gcgagctgcg ctggggggcg gcgcaaggat tggatagctg cctatatatg
    13141 acggtcggga caggcattgg tgtcggcgcc gtcgtggaag ggcgtctgct tcacgggatg
    13201 cttcatccag aaatgggaca tatgttcgtc cgccgccatc cggatgatgc gtttgccggc
    13261 atctgcccgt atcatggcga ctgtttggaa ggcatggcgt ccggcccggc gattgagcgg
    13321 cgttggggga aaaagggggc ggagctggcc aaccggagcg aggtgtggga gcttgaggcg
    13381 ttttacttag cccaagcgat cgccaactac atcctcattt tatcgccgaa aaaggtgatc
    13441 acgggcgggg gcgtcatgaa gcagacgcac gtgttgccgc ttgtgcgccg tcatgtgcag
    13501 gagttgctca atgggtatgt tcagcacgaa acgattctcg agaagatcga cgagtacatc
    13561 gtcctcccag ggcttggcga caacgccggt gttgcgggtg ccttggcgct cgcagcggcg
    13621 gcaaatgaat ag
//
