LOCUS AAXF02000034 8973 bp DNA linear BCT 04-AUG-2012 DEFINITION Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont531, whole genome shotgun sequence. ACCESSION AAXF02000034 VERSION AAXF02000034.1 DBLINK BioProject: PRJNA18191 BioSample: SAMN00627058 KEYWORDS WGS. SOURCE Bacteroides ovatus ATCC 8483 ORGANISM Bacteroides ovatus ATCC 8483 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. REFERENCE 1 (bases 1 to 8973) AUTHORS Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M., Liep,D. and Gordon,J. TITLE Draft genome sequence of Bacteroides ovatus (ATCC 8483) JOURNAL Unpublished REFERENCE 2 (bases 1 to 8973) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (15-FEB-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 3 (bases 1 to 8973) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-MAR-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans, it represents, on average, 0.034% of all 16S rDNA sequences and 0.071% of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were blasted against NCBI'snon-redundant (NR) database and predictions generated based on proteinalignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the contig level and may notnecessarily reflect any known order or orientation betweencontigs. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in August 2007, and the CDS comments were updated in January 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. FEATURES Location/Qualifiers source 1..8973 /organism="Bacteroides ovatus ATCC 8483" /mol_type="genomic DNA" /strain="ATCC 8483" /type_material="type strain of Bacteroides ovatus" /db_xref="ATCC:8483" /db_xref="taxon:411476" gene complement(1..1731) /locus_tag="BACOVA_00557" CDS complement(1..1731) /locus_tag="BACOVA_00557" /inference="protein motif:HMMPfam:IPR012944" /inference="similar to AA sequence:INSD:AAO75591.1" /note="COG: NOG26547 non supervised orthologous group" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q8A1G2|8.A.46.1.1" /protein_id="EDO13793.1" /db_xref="InterPro:IPR012944" /translation="MEEEMMKQYMKNKSLFLQKEELLSRIAELFSKKILRPSGVLVFLL ITCLGLFSCSKFLEENPKDKLPEDDVYNTISEVYLNAVASLYTYVGGYSDSQGLQGTGR GVYDLNTFTSDEAIIPTRGGDWYDGGFWQGLYLHDWGIENDAIQATWEYLYKVVMLSNK SLERIDKFAETHSATELPAYRAEVRAMRAMYYYYLMDLFGRIPLVQSSSVAMKDVVQSE RKTVFDFVVKELQEAAPLLSDAHSNQSGPYYGRITRPVVTFLLAKLALNSEVYTDNDWT DGQRPDGKNIKFTVNGSELNAWETVIYYCDQLKTMGYKLEPEYETNFSIFNEPSVENVF TIPMNKTLYTNQMQYLFRSRHYNHAKAYGLSGENGPSATIEALETFGYETAEQDPRFDI CYFAGIVHDLKGNIIKLDNGTVLEYLPWKVSLDITDTPYEQTAGARMKKYEVDPTATKD GKLMENDIVLFRYADALLMKSEAKVRNGANGDEELNEVRSRVNASPRTATLENILAERQ LELAWEGWRRQDLVRFGKFTRAYSSRPQLPDEASGYTTVFPIPEKIRVMNERLKQNPGY " gene complement(1731..4475) /locus_tag="BACOVA_00558" CDS complement(1731..4475) /locus_tag="BACOVA_00558" /inference="protein motif:HMMPfam:IPR000531" /inference="protein motif:HMMPfam:IPR012910" /note="COG: NOG26198 non supervised orthologous group; Psort location: OuterMembrane, score:9.49" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q45780|1.B.14.6.1" /protein_id="EDO13794.1" /db_xref="InterPro:IPR000531" /db_xref="InterPro:IPR012910" /translation="MVQHARLIFYSLLLLVIPCESTLAQKIPVTPIDSLITVGYATGSL KTLSGSVEKITETQMNKDQITNPLEAIRGRVPGLTIQRGSNGPAALDAVRLRGTTSLTS GNDPLIIVDGVFGDLSMLTSIYPTDIESFTILKDASETAQYGSRGASGVIEVTTKKGMS GRTQVAYNGSFGISTVYKNLKMLSGDEYRRIASERGISILDKGYNTDFQKEIEQTGLQQ NHHIAFYGGSSESSYRVSLGFMDRQGVILNEDMKNFTSNMNMNQKMFDGFLNCELGMFG SIQKNHNLVDYQKTFYSAATFNPTYPNHKDPVTNSWDGITTASQITNPLAWMEVQDDDA TSHISTHARLTFNLLEGLKLNLFGAYTYNIVENSQYLPTSVWANGQAYKGTKKRESLLG NMMLTYKKNWKKHFFDVLALAELQKETYTGYYTTVSNFSTDKFGYNNLQAGALRLWEGT NSYYDQPRLASFMGRFNYTYADRYVLTLNARTDASSKFGANHKWGFFPSASAAWVISEE EFMKQLPMVDNLKFRIGYGLAGNQSGIDSYTTLNLVKPNGVVPVGNSAVVSLGDLRNTN PDLKWEVKHTFNTGIDVALFGNRLLLSANYYNSRTTDMLYLYNVSVPPFTYNTLLANIG SMRNWGTEIAIGITPLKTKDMELNINANITFQRNKLLSLSGMYNGEMLSAPEYKSLSGL DGAGFHGGYNHIVYQMVGQPLGVFYLPHSTGLESDGNGGYTYGIADLNGGGVSLEDGED RYVAGQAVPKTILGSNISFRYKRFDLSLQINGAFGHKIYNGTSLTYMNMNIFPDYNVMK KAPKQNIKDQTATDYWLEKGDYVNFDYVTLGWNVPIEKVQKLKKYVRSLRLAFTVNNLA TISGYSGLSPMINSSTVNSTLGVDDKRGYPLARTYTLGLSINF" gene complement(4574..6649) /locus_tag="BACOVA_00559" CDS complement(4574..6649) /locus_tag="BACOVA_00559" /inference="protein motif:Gene3D:IPR013781" /inference="protein motif:HMMPfam:IPR001540" /inference="protein motif:superfamily:IPR008979" /note="KEGG: bth:BT0460 0. beta-hexosaminidase precursor K01207; COG: COG3525 N-acetyl-beta-hexosaminidase; Psort location: Periplasmic, score:9.44" /codon_start=1 /transl_table=11 /product="GH20" /protein_id="EDO13795.1" /db_xref="InterPro:IPR001540" /db_xref="InterPro:IPR008979" /db_xref="InterPro:IPR013781" /translation="MNIRKEYPKVCLFLWILGMCFHAHPILAQSVIPVPLKMEQGTGSF LLSEKTKLYTNLQGGEAELWENYLKALPVQLKEARMKDRKQMLFLLITPKTPQLPSPES YTLSVTSQRIEIRATSGAGLFYGMQTLLQLMQPASTGSYSVPSVEIEDTPRFAYRGLML DVSRHFSTKEFIKKQIDALAYYKINRLHLHLTDAAGWRLEIKKYPLLTDFAAWRTDPTW KKWWNGGRKYLRYDEPGASGGYYTQDDIREILEYARQHYITVIPEIEMPSHSEEVLAAY PQLSCSGEPYKNSDFCVGNEETFTFLENVLTEVMELFPSEYIHVGGDEAGKSAWKTCPK CQKRMKDEHLANVDELQSYLIHRIEKFLNNHGRRLLGWDEILQGGIAPNATVMSWRGEE GGIAAVTSGHHAIMTPGAYCYLDSYQDAPYSQPEAIGGYLPLKKVYAYDPVPASLTAEQ AKLVYGVQGNLWVEYIPTPEHVEYMIYPRMLALAEVAWSAPERKSWPDFHTRALSAVAD LQKKGYHPFDLSKEIGSRPESLQPVSHLALGKKVTYNSSYSPHYPAQGNTALTDGIRGD WTYGDGSWQGFISDNRLDVTIDMEKETPIHSITAAFMQVVGAEVFLPETVIISISDDGI NFTELQKQHFEVSKETPIRFTDISWQGEAKGRYVRYQAQAGSEFGGWIFTDEIIVK" gene complement(6649..8973) /locus_tag="BACOVA_00560" CDS complement(6649..8973) /locus_tag="BACOVA_00560" /inference="protein motif:Gene3D:IPR013781" /inference="protein motif:HMMPfam:IPR000421" /inference="protein motif:HMMPfam:IPR001540" /inference="protein motif:superfamily:IPR008979" /note="KEGG: bth:BT0459 0. beta-hexosaminidase precursor K01207; COG: COG3525 N-acetyl-beta-hexosaminidase; Psort location: Periplasmic, score:9.76" /codon_start=1 /transl_table=11 /product="GH20" /protein_id="EDO13796.1" /db_xref="InterPro:IPR000421" /db_xref="InterPro:IPR001540" /db_xref="InterPro:IPR008979" /db_xref="InterPro:IPR013781" /translation="MKQLFKLTGCLALAGLFASCQSVQQEANYQIIPMPQEIVTAQGSP FILKSSVKILYPEGNEKMQRNAKFLADYLKTATGKDFAIEAGTEGKNAIVLALGTENEN PESYQMKVTGDGITITGPTEAGVFYGIQSLRKSLPVAVGADIAMPAVEINDAPRFGYRG AHFDTSRHFFTVDEIKTYIDMQALHNMNRLHWHITDDQGWRLEIKKYPKLTEIGANRTE TVIGRNSGEYDGKPYGGFYTQEQAKEIVDYAAERYITVVPEIDLPGHMQAALAAYPELG CTGGPYEVWRQWGVSEDVLCAGNDQVLKFLEDVYGELIEIFPSQYIHVGGDECPKVRWE KCPKCQARIKALGLKSDQSHSKEERLQSFVINHIEKFLNDHGRQIIGWDEILEGGLAPN ATVMSWRGEKGGIEAAKQKHDVIMTPNTYLYFDYYQAKDIENEPFGIGGYLPMERVYSY EPMSASLTPEEQKYIKGVQANLWTEYIATFPHAQYMVLPRWAALCEIQWSSPEKKNYAN FLSRLPQLIKWYDAEGYNYAKHVFDVQAEFDPNPAEGTMDVTLSTIDGAPVYYTLDGTE PTAASPVYEGVLKIKENVTLSAKAIRPNGESKTVTEKIDFSKSSMKPIVANQPINEQYL FKGASTLIDGLKGNSSYKSGRWIAFNGNDMDMTIDLQQPTEISSVAISTNVAKGDWVFD ARNLSVETSDDGKTFKKIASEEYPEMKETDKDGIVEHKLTFAPVTTQYVRVIASPEKSL PAWHGGKGKNAFLFVDEIKID" ORIGIN 1 ctaatacccc ggattctgtt tcaacctctc attcatcacc cgtattttct ccggtatagg 61 gaatacagtt gtatatccac ttgcctcatc cggtagttgc ggacggctgc tgtaagctct 121 tgtaaacttc ccgaagcgga ctaagtcctg tcgtctccat ccttcccaag ccagttccag 181 ctgacgttcg gcaaggatat tctctaaagt ggcagtgcgg ggagaagcgt tgacacggct 241 acgaacttca ttgagttctt catctccgtt tgctccgttc cggactttcg cttcactctt 301 catcaacaaa gcgtctgcat agcggaacaa tacaatgtca ttctccatca acttaccgtc 361 ttttgtggca gtcggatcaa cttcatactt tttcatgcgg gctcctgcgg tttgttcgta 421 tggggtatct gttatatcca gagatacttt ccagggcaga tattccagta ccgttccatt 481 atccagtttg attatgtttc ctttcaggtc gtggacaatt cctgcaaaat agcaaatatc 541 aaaacgggga tcttgttccg cggtttcata accgaaagtt tcgagggctt caatggtggc 601 gctgggaccg ttctcaccgc ttagtccgta agccttggcg tgattatagt ggcgggaacg 661 gaagagatat tgcatctggt tggtgtacag agttttattc atcgggatag tgaagacatt 721 ctctacggac ggttcgttga atatagagaa attcgtttcg tattccggtt ccagtttgta 781 gcccatagtc ttgagttggt cacaatagta gatgactgtt tcccaggcat ttagttcgct 841 gccgttcact gtgaacttta tattttttcc atccgggcgt tgtccgtccg tccaatcatt 901 gtctgtataa acttcggagt tcaaagccag tttggccagt aggaaagtta ctacggggcg 961 agtgatacgg ccatagtaag ggccggactg gttgctatgt gcatcgctca acaaaggagc 1021 cgcctcttgc aattccttga caacaaagtc aaagaccgtt tttcgttcac tttgcactac 1081 atctttcatt gctacggaag aggattgaac tagcgggatg cgtccgaaca aatccatcag 1141 gtaataataa tacatagccc gcatggcacg tacttcggca cggtatgccg gcagttccgt 1201 agcggaatga gtttcggcga atttgtctat ccgttccagt gatttgttac ttaacatgac 1261 gactttatag agatattccc atgtagcctg aatggcatca ttctctattc cccaatcgtg 1321 caggtaaagt ccttgccaaa aaccaccgtc ataccagtca cctccacggg tggggataat 1381 ggcttcgtcg gaggtaaagg tgttcaggtc gtagactccc ctacccgttc cctgcaatcc 1441 ctgactatca ctataaccgc ctacgtatgt ataaagcgaa gctactgcat tgagatatac 1501 ttccgagatt gtattgtaaa cgtcatcttc gggtaactta tctttcggat tttcctccaa 1561 aaacttacta catgagaata aacccaggca cgttataaga aggaatacca gcactcccga 1621 aggacgtaag atcttctttg aaaacagttc cgctatcctt gaaagcagtt cttctttctg 1681 caaaaaaaga cttttatttt tcatatattg tttcatcatc tcctcctcca ttaaaagtta 1741 atactcaatc ccagtgtgta cgtccgcgcc aacggatatc cccgtttatc atccacgcct 1801 aaagtggagt tcaccgttga actgttaatc atgggtgaaa gtcccgaata accggaaatg 1861 gttgccaggt tgttgactgt aaatgccaga cgcagggagc ggacatattt cttcagtttc 1921 tgtactttct caatcggcac gttccaaccc agcgtcacat agtcgaagtt gacataatct 1981 cccttttcca accaatagtc ggtggcggtt tgatccttga tgttctgttt cggggctttc 2041 ttcattacgt tatagtcggg aaatatattc atgttcatat aggtcaggga ggttccgttg 2101 tagattttat gtccgaaagc tccgttaatc tgcaaagaca gatcgaaacg cttgtagcgg 2161 aaactgatgt tggagccaag aatcgtttta ggtacggctt gtcctgccac gtagcggtct 2221 tcaccatctt caaggctgac acctccgcca ttcagatcgg cgatgccata ggtgtatccg 2281 ccgtttccgt cggattccag tcccgtactg tggggaagat agaacacgcc caacggttga 2341 cccaccatct gatatacgat gtggttgtat cctccatgaa agccggctcc gtcgaggcca 2401 gaaagacttt tatattcggg agcagaaagc atttcgccgt tgtacatacc gcttaatgag 2461 agcagtttgt tgcgctggaa agtgatattg gcattgatat tcagttccat atccttggtc 2521 ttcaaggggg tgatgccgat ggcgatttcc gtaccccagt tgcgcatgga accgatgttg 2581 gcaagcaagg tgttataggt gaaaggcggt acgcttacgt tgtaaaggta aagcatgtct 2641 gtggttcggg agttgtaata gttggcggaa agcagcaggc ggttgccgaa aagagctacg 2701 tcgataccgg tattaaaggt atgtttcact tcccatttca ggtcgggatt cgtgttccgc 2761 aagtctccta aagatacgac ggcggagttt ccgacgggga ctacaccgtt cggctttaca 2821 aggttcaatg tagtgtatga gtcaatccca ctctgattac ctgccagacc gtagccaata 2881 cggaatttca gattatccac catcggcaac tgcttcataa attcttcttc actaatcacc 2941 catgcggcag atgcggaggg gaagaatccc catttatggt tcgctccgaa tttggaggaa 3001 gcatctgtac gggcgttcag cgtcagtacg tagcggtcgg cgtatgtata gttgaaacgt 3061 cccatgaaag atgccagacg gggttggtcg tagtaagaat tagtgccttc ccacaagcgg 3121 agtgcgcctg cctgaagatt attatacccg aatttgtcgg tgctgaaatt gcttactgtt 3181 gtataatatc ccgtgtaggt ttctttttga agttcggcaa gtgccagcac gtcaaagaaa 3241 tgttttttcc agtttttctt ataggtcagc atcatgttgc ccagcaagga ttctcgcttt 3301 ttggttcctt tgtaagcctg gccgttcgcc cagacggaag tgggaaggta ttgcgaattt 3361 tccacgatat tataggtata ggcaccgaag aggttaagtt tcagcccttc cagcagattg 3421 aaagtcagac gggcgtgcgt gctgatgtgc gaagtggcat catcatcctg cacttccatc 3481 catgccaatg gattggttat ttggctggcg gttgttattc cgtcccagga attggtgacg 3541 gggtctttat ggttgggata ggtgggattg aatgtggctg ccgaatagaa cgttttctga 3601 taatccacca gattatgatt cttctgaatg gaaccgaaca ttcccagctc gcagttcagg 3661 aagccatcga acattttctg attcatgttc atattcgaag tgaagttttt catgtcttca 3721 ttcaaaatca ctccctgacg atccatgaaa ccaagagaaa cgcgatagct ggactcactg 3781 gaaccaccat aaaaggcgat gtgatggttt tgttgtagtc ctgtctgttc aatctctttc 3841 tgaaaatcgg tattatatcc tttgtccaga atggaaatcc cgcgttccga agcgatacgg 3901 cggtactcat cgccggacag cattttcaga ttcttgtaga cggtggaaat tccgaagcta 3961 ccgttgtagg ctacttgtgt tctgccgctc attccttttt tagtggtgac ttcgatcaca 4021 ccggaagctc ctcgtgaacc gtattgtgcg gtttcggaag cgtctttcaa aatggtgaag 4081 ctctcaatat ccgtagggta aatggaggtg agcatgctca agtcgccaaa tactccgtcg 4141 acgattatca aagggtcatt gccactggtc agtgaggtag ttccccgcag gcgtaccgca 4201 tccaaggctg ccggaccgtt tgatccgcgt tggatggtca agcccggcac acgtccgcgg 4261 attgcttcca aagggttcgt tatctggtct ttattcatct gtgtctccgt aatcttctct 4321 acggaaccgg agagggtttt caaacttccg gtggcatatc caacagttat caatgagtct 4381 atgggcgtga cgggaatctt ctgcgccaag gtgctctcgc acgggattac cagaagaaga 4441 aggctgtaga aaatgagtct agcgtgttgt accatattct tagtttttaa ggtaataata 4501 caaatataac aaaaaaagag ggaatctggt tgagtagatt ccctctttta ttcataaatg 4561 atattgacaa tgtctatttc acgattatct catccgtaaa tatccaacct ccgaattcgc 4621 ttccggcctg tgcctggtaa cgtacgtatc ttcctttggc ttctccctgc catgaaatat 4681 ccgtaaaccg aataggggtt tctttgctca cttcaaagtg ttgtttttgc aactccgtga 4741 agttaattcc atcatcggaa atggaaataa tcactgtctc cggcaggaat acttccgcgc 4801 cgaccacttg cataaaagcg gcggtgatgg aatggatagg ggtttctttt tccatatcaa 4861 tcgtcacatc gagacggttg tccgagataa atccttgcca tgaaccatca ccgtaagtcc 4921 agtcaccacg tatgccgtct gtcagggctg tgttgccttg tgcgggataa tgggggctat 4981 aggaggagtt ataggtcact tttttaccga gcgccaaatg gctgactggt tgaagagatt 5041 cgggacggct gccgatttct ttgctcaaat cgaaaggatg gtatcctttt ttctgtaaat 5101 cggccactgc tgacagtgcg cgggtatgga aatcgggcca cgacttacgt tccggagccg 5161 accaggctac ttccgccaat gccagcatac ggggataaat catatattcc acgtgttcgg 5221 gagtggggat atattccacc cataggttac cctgcacacc atagactaac tttgcctgct 5281 ccgcagttaa ggaagcgggg acaggatcgt aggcatatac tttcttcaat ggcaggtagc 5341 caccgatagc ttccggctgg gagtacgggg cgtcctgata gctgtccaga taacagtatg 5401 cacccggtgt catgatggca tgatggccgg aggtgacggc agcaatacca ccctcttctc 5461 cacgccacga cataacggtt gcattgggcg cgatacctcc ttgcagaatc tcgtcccatc 5521 ccaagagacg gcgtccgtga ttgttgagga acttttcgat acggtgtatc agatagcttt 5581 gcagctcgtc tacattggcc agatgctcgt ctttcattct cttctgacat ttcggacagg 5641 tcttccaagc cgatttgccg gcctcatcac ctcctacatg aatgtattcg gaagggaaaa 5701 gttccatgac ttccgtcagt acattctcca gaaaggtgaa agtttcttca ttaccgacac 5761 agaaatcgga gttcttgtaa ggttctcccg aacaggatag ctgcgggtag gcagccagca 5821 cttcttccga atgggaaggc atttcaatct ccggaatcac cgtgatgtaa tgctgacggg 5881 catattccag tatttcacga atatcgtcct gggtgtaata gccgccggaa gctccgggtt 5941 catcataacg gaggtattta cgtccgccgt tccaccattt cttccaggtg gggtctgtac 6001 gccaggctgc aaaatcggtc agtaaagggt atttcttgat ttcaagccgc cagcctgccg 6061 catcggtcag gtgcaggtgc aaacggttga ttttgtaata tgccaatgcg tctatctgct 6121 tctttataaa ctctttggta gagaaatgac gggatacgtc cagcatcagt ccgcgataag 6181 caaaacgagg ggtgtcttct atctcgacag agggtacgga atagctgcct gtgcttgccg 6241 gttgcatcag ctgtaacagg gtttgcattc catagaataa tccggctccc gaagttgccc 6301 ggatctcaat tcgttgagag gtgacggaga gtgtatagct ttccggtgat ggcaattggg 6361 gggtcttggg ggttatcaat aagaaaagca tctgtttcct gtctttcatt ctcgcttctt 6421 tcagctgaac aggtaatgct ttcaggtaat tctcccagag ttccgcttct ccaccttgca 6481 ggtttgtata aagtttagtc ttttctgaca gtaaaaaaga gcccgtcccc tgctccattt 6541 ttaatgggac aggtatgacg gactgtgcca aaatgggatg tgcatggaag cacatcccca 6601 atatccataa gaaaagacaa actttagggt attcttttct tatattcatt aatcaatttt 6661 gatttcgtct acaaataaaa atgcgttttt accttttcct ccatgccatg ccggaagtga 6721 tttctccggt gaagcaatga ctctcacgta ttgggtggtc acaggagcaa aggttagttt 6781 gtgctcaacg attccgtctt tgtctgtctc tttcatctcc ggatattctt cggaagcaat 6841 ctttttgaag gtcttgccat catctgaagt ttctacggac aggtttctgg catcaaatac 6901 ccagtcccct tttgctacat tggtagagat tgccacgctt gagatttcag taggttgctg 6961 gaggtcgata gtcatatcca tgtcgtttcc gttgaaagcg atccaacggc ctgacttata 7021 gctgctattt cctttcagac catctatcaa agtggaagct cctttgaaca ggtattgttc 7081 attgataggc tgattggcta caatcggctt catgctggat ttgctgaaat caatcttctc 7141 tgtcactgtc ttgctttcgc cgttgggacg aatggctttg gcggacaggg ttacattttc 7201 cttgattttc aatactcctt cgtaaacggg ggaagcggca gtaggttctg tgccgtctaa 7261 tgtatagtaa acaggggcac cgtcgatggt agacagggta acgtccattg ttccttctgc 7321 cggattcggg tcaaactcgg cttgtacgtc aaatacgtgt ttagcgtaat tgtatccttc 7381 tgcatcgtac cacttaatca gttgtggaag acgggacagg aagttggcat agtttttctt 7441 ttcggggctg gaccactgaa tttcacacaa agcagcccaa cgaggcaata ccatgtattg 7501 tgcatgcggg aaggtagcga tgtattctgt ccagaggttg gcttgcactc ccttgatgta 7561 tttctgttct tccggagtaa gagaagccga catcggctcg tagctataca ctctttccat 7621 cggcagataa ccaccgatac cgaacggttc gttttcgatg tctttagctt ggtagtagtc 7681 gaaatacaga taggtgttcg gtgtcatgat gacatcgtgt ttctgcttcg cagcttcgat 7741 accacctttc tcaccacgcc atgacatcac ggtagcattc ggagcaagtc cgccttcgag 7801 gatttcatcc caaccgatga tttgacgtcc gtggtcgttc aggaatttct caatatggtt 7861 gatgacaaag ctctgcaaac gttcttcttt gctgtggctc tgatctgact tcaatcccaa 7921 tgctttgata cgtgcctggc acttcggaca tttttcccat cttactttcg ggcattcgtc 7981 gccccctaca tgaatatatt gtgatgggaa gatttcaatc aattcaccat aaacatcttc 8041 caagaatttc aatacttggt cgtttccggc gcaaagcaca tcctcggata caccccattg 8101 tctccatact tcgtatggac caccggtaca acccagttca gggtaagcgg caagggcagc 8161 ttgcatgtgt cccggaaggt cgatttcggg aacaacggtg atgtagcgtt cggctgcata 8221 atctacaatt tctttggctt gttcctgggt gtagaatcca ccgtaaggtt tgccgtcgta 8281 ttcgccggag ttacgtccga tgactgtttc tgttctgtta gcaccgattt cagtcagttt 8341 cgggtatttt ttgatttcca gacgccaacc ttgatcgtcg gtgatatgcc agtggaggcg 8401 gttcatgtta tgtaaagcct gcatgtcgat gtaggtcttt atttcatcca cggtaaagaa 8461 atgacggctg gtatcgaaat gcgcgccacg atagccaaag cggggagcgt cattgatttc 8521 tacggctggc atggcaatat ccgcaccgac agccacaggc aatgatttgc gcagggactg 8581 gatgccatag aatacgccgg cttccgtagg accagtaatc gtgattccgt caccggtaac 8641 tttcatctgg taggattccg gattttcgtt ctccgtgcct aatgccagta cgattgcgtt 8701 tttaccttct gttccggctt caatggcgaa atcttttccg gtagccgttt tcagatagtc 8761 tgccaggaat tttgcgttgc gttgcatctt ctcgtttcct tccggataga gaattttcac 8821 actgctcttg aggatgaagg gacttccttg agcggttaca atttcttgtg gcatggggat 8881 gatctggtag ttggcttcct gttgcaccga ttggcaagag gcaaaaagtc ctgctagtgc 8941 cagacatccg gttaatttaa agagttgctt cat //