LOCUS NZ_DS264579 9403 bp DNA linear CON 25-NOV-2019 DEFINITION Bacteroides ovatus ATCC 8483 Scfld0226, whole genome shotgun sequence. ACCESSION NZ_DS264579 VERSION NZ_DS264579.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN00627058 Assembly: GCF_000154125.1 KEYWORDS WGS; RefSeq. SOURCE Bacteroides ovatus ATCC 8483 ORGANISM Bacteroides ovatus ATCC 8483 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. REFERENCE 1 (bases 1 to 9403) AUTHORS Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M., Liep,D. and Gordon,J. TITLE Draft genome sequence of Bacteroides ovatus (ATCC 8483) JOURNAL Unpublished REFERENCE 2 (bases 1 to 9403) AUTHORS Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M., Liep,D. and Gordon,J. TITLE Draft genome sequence of Bacteroides ovatus (ATCC 8483) JOURNAL Unpublished REFERENCE 3 (bases 1 to 9403) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-MAR-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 4 (bases 1 to 9403) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-MAR-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 5 (bases 1 to 9403) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (15-FEB-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 11/25/2019 16:35:53 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Method :: Best-placed reference protein Annotation Software revision :: 4.10 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; Genes (total) :: 4,931 CDSs (total) :: 4,837 Genes (coding) :: 4,690 CDSs (with protein) :: 4,690 Genes (RNA) :: 94 rRNAs :: 4, 4, 6 (5S, 16S, 23S) complete rRNAs :: 4, 3, 5 (5S, 16S, 23S) partial rRNAs :: 1, 1 (16S, 23S) tRNAs :: 78 ncRNAs :: 2 Pseudo Genes (total) :: 149 CDSs (without protein) :: 147 Pseudo Genes (ambiguous residues) :: 1 of 149 Pseudo Genes (frameshifted) :: 75 of 149 Pseudo Genes (incomplete) :: 69 of 149 Pseudo Genes (internal stop) :: 17 of 149 Pseudo Genes (multiple problems) :: 14 of 149 ##Genome-Annotation-Data-END## REFSEQ INFORMATION: The reference sequence was derived from DS264579. Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans, it represents, on average, 0.034% of all 16S rDNA sequences and 0.071% of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans, it represents, on average, 0.034% of all 16S rDNA sequences and 0.071% of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were blasted against NCBI'snon-redundant (NR) database and predictions generated based on proteinalignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the contig level and may notnecessarily reflect any known order or orientation betweencontigs. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in August 2007, and the CDS comments were updated in January 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline (PGAP) set; GeneMarkS-2+ repeat_region FEATURES Location/Qualifiers source 1..9403 /organism="Bacteroides ovatus ATCC 8483" /mol_type="genomic DNA" /strain="ATCC 8483" /isolation_source="biological product [ENVO:02000043]" /host="Homo sapiens" /type_material="type strain of Bacteroides ovatus" /db_xref="ATCC:8483" /db_xref="taxon:411476" /note="type strain of Bacteroides ovatus ATCC 8483" gene 1..816 /locus_tag="BACOVA_RS11930" /old_locus_tag="BACOVA_02741" CDS 1..816 /locus_tag="BACOVA_RS11930" /old_locus_tag="BACOVA_02741" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004298557.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH16" /protein_id="WP_004298557.1" /translation="MKKVALFLFLSVCFAQQSCSSDSVGTEPEENPQDILFKDDFNFFD EKVWTKETHEPGWTNQELQAYDAAHVSVGKDGDKSVLILTAERKGNKIYSGRINSKGKK SFKYRKIEASIKLPKTNGGLWPAFWMMGDNDKQWPACGEIDIMEMGEQSGMAAGDSEKQ VNTAIHYGPSAAAHEQQYYKANVANSLQDGNYHTYSLDWDENNLTISIDNVKFHTFDIS SNTYFHDNFYILFNLAVGGAFTGITDINKLTGLKDGQKVNMYIDWVKIL" gene 837..4025 /locus_tag="BACOVA_RS11935" /old_locus_tag="BACOVA_02742" CDS 837..4025 /locus_tag="BACOVA_RS11935" /old_locus_tag="BACOVA_02742" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004298558.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q93TH9|1.B.14.6.2" /protein_id="WP_004298558.1" /translation="MNMEKCKYLLFCGTLALWMAGSFQQVYAATEPSSQAIQQQSNKVT GKVSDATGPIIGASVVEKGTANGTITDLDGNFSLNVKAGATLVVSYVGYKSEEVKAGRG PLNITLKEDAKALDEVVVTALGIKRERKALGYGIDEVKGEALTKAKETNLINSMAGRVP GLVVSQTAGGPSGSTRVILRGSTEMTGNNQPLYVVDGVPLDNTNFGSAGTNGGFDLGDG ISSINADDVENMSVLKGPAASALYGSRASHGVILITTKRANKDKISVEYNGTLTFDTQL AKWDEVQQIYGMGSNGTYSYDAISNTNKSWGPKADGSNMLKYFDGVERPFLIVPDNTSN FFRTGITATNSAIIGVNSGKTGIRFTYTDMRNKDIVPQTHMSRDIFNLRANTSAGKVDL DFSVNYTREDVKNRPALGDSKSNIGKNLMTLATTYDQEWLQTYQTADGEYSNWNGMDPY NVNPYWDIYKNFNKSKKDLFRMNGKAVWNIDPHLKLQATLGAELNWFTFDDYKAPTTPG FEAGRLQNSAFRNRMYNFEALALYNNHWGDFDFNATLGGNVYKVNNQTTVTTAQDMKIR DVPSLTSFNEISVVPGSYRKQINSVYGAVNVGWKHMLYLDATLRGDQSSTLPTGNNMYV YPSFSGSFVFSELTKLGDLLPYGKVRMSWAQVGSDTDPYQLGLVYTKSKYAYPGYTIGY IDNGTIPNKDLKPTKTNSVEMGLELKFLKNRIGLDFTYYSQISKNQIMGMASSWTTGYN YRLINAGKIENKGIEIALSTRPIQTRDFSWDINLNFSKNSNKVKELDGESDMFELEKAS WLDVQVAAKVGENFGSIVGPDFQRNEKGDILIDPQTGLPQYDKSNHVLGNASWDWTGGL LTNFTYKNLSLVAVFDVKVGADLYSMSARAAYESGKSPETLAGRDAWYRSEEERLAAGI AKGADNWKPTGGFVAPGVIDNGDGTYRPNDIYINPEDYWMSVCRNAPSMFIYDNSYVKC RELTLSYNVPKSWLKNVVSGLTVSFVARNPFIVWKNIPNIDPDSNYNNTTGMGLEYGSL PSRRSYGFNVNVKF" gene 4037..5713 /locus_tag="BACOVA_RS11940" /old_locus_tag="BACOVA_02743" CDS 4037..5713 /locus_tag="BACOVA_RS11940" /old_locus_tag="BACOVA_02743" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004302737.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q8A0N7|8.A.46.2.2" /protein_id="WP_004298559.1" /translation="MRQSKYITIITMACALFFASCSDEYMENMNTDPSKAATIDPNAQL TTAQLQTYGDLSMMEIYRNYHYAFTQQLMGCWNTTNYGGRHTLDNNEMSRIWTSFYTQS LKNIIDAQYRTAEDAEKVNINSVLRIYRVYLMSIITDTYGDAPFSEAGLGFLEGKFNPK YDKQEDIYNAFFLELEDAVNKIDPTKDKVTGDLIYAGDVTKWQQLANSLRLRFAMRISS VNPTKAQTEFENALAANGGVITDASSDALIKYMTIAFSFGQEAYSDYRGNSLSQLLFGN DPANNPSYLCSTFFNQLYNSGDPRTFKISRCYYDGLMSATSPDNRVDITQEMIEKGIAF SPRDPGAYSWEPWPTGYDSDICAELAVNNPSVTATMAREVEPKLANNFLKSDNPGVVMT SAEVKFLMAEATVKKWNVGSVSAEDLYKQGVRAAIDFLTDNYGCTATTDAEFDAFIQDK GAFGHTDNQKLEAINTQAWILHFTNPAECWANVRRSGYPKLKSPAEYGFGQYLTGGTEI PVRLCYPVLESSYNKKSYNEAIERMGGTDNWHSLLWWDTEN" gene 5734..6996 /locus_tag="BACOVA_RS11945" /old_locus_tag="BACOVA_02744" CDS 5734..6996 /locus_tag="BACOVA_RS11945" /old_locus_tag="BACOVA_02744" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004298560.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_004298560.1" /translation="MKKIYIALFALLAVGSTFTACTEEEPFATVTENDDPHILAPVFPD RTNGQLATFANISRDANLSIALTVTPKDYTTVTWFIDGQEVESGTDSDKEINRSLKAGT YNLKIEVETVKGKKTSREGLVVVNPLADDPQSKEVAFERIVSPGKTARLYGSNLQNVTA ILLGGNTITDPTYVESADENYLEYTIPTGVSEGDYRIVLQDADGNQYGADMVKVTNASL VISGANRATANVDWTISGINLENIASLTIGGQTVSQFSNQSSTEITLTCPDLSDGSYTM TGKTRSGEAVQFLNDNITTTEQTVTVSTEITLWSGHHYVSWDKPDGDPNKTFGLIPMDV FAGITAGSTLKVVYSIEPTAEYHKMQLATGYWTGLASEMEFTENGEYTLILTQDMLNKI QAEAGFLCVGHGYYVDLVTVK" gene 7109..9403 /locus_tag="BACOVA_RS11950" /old_locus_tag="BACOVA_02745" CDS 7109..9403 /locus_tag="BACOVA_RS11950" /old_locus_tag="BACOVA_02745" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004298561.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH3" /protein_id="WP_004298561.1" /translation="MKLRNIILMTASIAMTACSKQAVPTAIPEDAKIEQQVEELLSKMD LDAKIGQMTELAIDVLGETINGEFQLDEAKLHKAIAEYKVGSFLNAPGPVAQSPEKWNE IIGRIQELSMAEIGIPCIYGLDQNHGTTYTLGGTLFPQNINMGAAFNPDLTYEAARVTA YETRASNCPWTYSPTVDMARDPRWPRVWENYGEDCLVNAIMGSTAVRGFQGDDPNHIPA DRIATSVKHYMGYSMPRTGKDRTPAYISVSELREKCFAPFRACVEAGALTIMVNSGSIN GKPVHADRELLTQWLKEDLGWDGMLITDWADINNLYTREHVAANKKEAIEMAINAGIDM AMEPYDLNYCTLLKELVQEKKIPMSRIDDAVRRVLRLKFRLGLFDHPNTLLKDYPLFGS KEHALIALHAAEESEVLLKNKDNILPLPQGKKLLVTGPNANSMRCLNGGWSYSWQGHLT DRFADKYNTIYEAICNKFGADHVRLEQGVTYKPEGAYMEENEPEIEKAVAAARNVDIII ACIGENSYCETPGNLSELAISASQSKLVKALAATGKPIILILNEGRPRIINELEPLADA VIDILLPGNYGGDALANILAGDVNPSAKMPYTYPRHEAALTTYDYRVSEEMDKMEGAYD YNAVVSVQWPFGYGLSYTTFEYSNFQTDKSSFTAGDDLHFSIDVTNTGKYAGKEVVMLF SSDLVASLTPENRRLRAFKKVELQPGETQTITLSIKGSDLAFVNSDGQWVLEQGDFRMQ CGNQTLNIICK" ORIGIN 1 atgaaaaaag tagcactatt cctattttta tccgtttgct tcgcgcaaca atcctgttcg 61 tcggattctg ttggaacgga accggaagaa aatccacaag acattctttt taaagacgat 121 ttcaactttt tcgacgaaaa agtatggacg aaagaaactc atgaacccgg ttggaccaac 181 caagagctac aggcatacga tgccgctcat gtatcggtag gaaaagacgg ggataaatcg 241 gttctgattc ttaccgccga acgcaaaggg aataaaatct attcaggcag gatcaattca 301 aaaggaaaaa agagtttcaa gtatcgcaag atagaagcaa gcatcaagct tcccaagact 361 aacggcggac tatggcctgc tttctggatg atgggagaca acgacaaaca gtggccggca 421 tgcggggaga ttgatattat ggaaatggga gaacagagcg gaatggctgc gggcgattcg 481 gaaaaacaag tgaacaccgc cattcactat ggccccagcg cagcggctca cgaacagcaa 541 tactacaaag ccaatgttgc caacagccta caagacggca attatcacac ctactccctg 601 gattgggatg aaaacaacct gacaatatcc attgacaacg tcaagtttca tacgttcgac 661 attagctcga acacatactt tcatgacaac ttctacatcc tgttcaacct cgccgtggga 721 ggtgcgttta caggaattac cgatattaat aaactgacgg gcctgaaaga cggtcagaaa 781 gtgaatatgt acatcgactg ggtgaaaata ttataggaat cttaaatata catgctatga 841 atatggaaaa atgtaagtat ctactgtttt gcggaacact cgccctttgg atggcgggaa 901 gtttccagca agtgtatgca gcaacggaac caagttccca agctattcaa caacaaagca 961 acaaggtaac cggaaaagtg agcgatgcca caggtcccat cattggggct tccgtagtgg 1021 agaaaggaac tgccaacgga acgattaccg atctggatgg aaatttcagt ttgaatgtaa 1081 aagcgggagc tacgctcgtt gtcagctacg tgggttataa atccgaagaa gtaaaagcgg 1141 gaagaggtcc tttgaacatc accttgaaag aggatgccaa agcattggat gaagtggtag 1201 ttaccgccct tggcatcaag agagagcgca aagcgttggg ctacggcatt gatgaagtga 1261 aaggtgaagc cttgaccaaa gcgaaagaga cgaatctcat caactccatg gcaggacgtg 1321 tgccaggctt ggtagtcagc cagaccgccg gtggtccttc cggttctacg cgcgttattc 1381 tgcgaggcag taccgaaatg accggcaata accagcctct ttatgtagtg gacggtgtac 1441 ctttggacaa cactaatttc ggcagtgccg gcacaaacgg aggcttcgac ttgggcgacg 1501 gtatttcgag catcaatgcc gatgacgtag aaaacatgtc ggtattgaaa ggccctgcgg 1561 catctgcact ttatggtagc cgtgccagcc acggtgttat cttgattaca accaagcgag 1621 ccaacaagga taaaatcagc gtggaatata acggaacgct gactttcgac acccaacttg 1681 ccaaatggga tgaagtgcaa cagatatatg gtatgggaag caacggtact tatagctatg 1741 atgccatatc caacacaaac aaaagttggg gccccaaagc ggacggttca aacatgttga 1801 agtatttcga cggcgtagaa cgccctttcc tcattgtccc cgacaatacg tccaacttct 1861 tccgcacagg tatcaccgcc accaactccg ccatcatagg tgtgaacagc ggaaaaaccg 1921 gaattcgctt tacctatacg gatatgcgca ataaagacat tgttccccag acgcacatga 1981 gccgtgacat tttcaacctg cgcgccaata cgtctgcagg aaaggtagac ttggatttca 2041 gtgtcaacta tacacgggaa gacgtgaaga accgtccggc tctgggcgac agcaagtcca 2101 atatcggtaa aaacctgatg acccttgcca ccacctacga ccaggaatgg ttgcaaacct 2161 atcagactgc cgacggcgaa tattccaact ggaacggcat ggatccctac aacgtgaatc 2221 cgtactggga tatctataag aacttcaaca aatccaagaa agacttgttc cgcatgaacg 2281 gcaaggccgt atggaacatc gacccacatc tgaaacttca ggcaacgctg ggtgccgaac 2341 tcaactggtt cacgttcgat gattacaaag cacctaccac tcccggattc gaagccggaa 2401 gacttcaaaa cagtgctttc cgcaaccgca tgtacaactt tgaagcgttg gctctctaca 2461 acaaccattg gggtgatttc gatttcaatg ctacattagg cggcaatgta tataaggtga 2521 acaaccagac caccgttacc accgctcaag acatgaagat acgcgatgtc ccttcattga 2581 cgagcttcaa cgagatcagt gtagtgcccg gcagttaccg caagcagatt aattcggttt 2641 acggagcagt gaacgtggga tggaaacaca tgctttacct cgatgctacg ttgcgtggcg 2701 accaatcgtc tacccttccc actggcaaca acatgtatgt gtatccttct ttctccggta 2761 gctttgtatt ctccgagctg acaaaactgg gcgacctcct gccttacgga aaggtgcgta 2821 tgtcatgggc ccaagtaggt agcgataccg atccttatca attgggactc gtctatacga 2881 aatccaaata tgcttatccc ggatatacca tcggatatat cgataacgga accatcccca 2941 acaaagactt gaaaccaacc aaaacaaact ccgtggaaat gggattggaa ttgaaattcc 3001 tgaagaaccg tatcggactg gatttcacct actattctca aatcagtaaa aaccagatta 3061 tgggaatggc aagttcctgg acaaccggtt acaactatcg cttgattaat gccggcaaga 3121 tagaaaacaa aggtattgag attgccctta gcacacgacc gattcaaaca cgcgattttt 3181 cctgggacat taatctgaac ttctccaaaa acagcaacaa agtaaaagaa ctggatggcg 3241 aatcagatat gtttgaactg gaaaaggcgt cttggctcga cgttcaggtg gccgctaagg 3301 taggcgagaa ctttggttct attgtaggcc cggacttcca acggaacgaa aagggagata 3361 ttctgattga cccgcaaacc ggtctgccgc aatatgacaa gagcaaccat gtattaggta 3421 atgcctcttg ggactggaca ggtggattac tcaccaactt tacctacaag aatctctcgc 3481 tggtagcagt attcgatgtg aaagtaggcg ccgacctcta ttctatgtct gcccgtgctg 3541 cttacgaatc gggtaaaagt ccggaaacat tggcaggacg cgacgcctgg tatcgctcgg 3601 aagaagaaag attggcagcc ggcattgcaa aaggagccga taactggaag ccgacaggcg 3661 gattcgttgc tccgggtgtc atcgacaacg gtgatggaac atatcgtccc aacgacatct 3721 acatcaatcc ggaagactac tggatgagcg tttgccgtaa tgcaccttct atgtttattt 3781 acgacaactc atacgtgaaa tgccgtgagc tgacgttgag ttacaatgta cccaaatcct 3841 ggctgaagaa tgtagtaagc ggactgaccg tttcgttcgt tgcccgcaat ccgttcatcg 3901 tatggaagaa tatcccgaat atcgacccgg attccaacta caacaatacc accggtatgg 3961 gacttgaata cggttcgttg ccttcacgca gaagctatgg ttttaatgta aatgtgaaat 4021 tctaaaaaga tatatcatga gacaatctaa atacatcaca atcatcacga tggcatgtgc 4081 cctgtttttc gcttcttgca gcgatgaata catggaaaac atgaataccg acccttccaa 4141 ggccgctacc atcgacccga atgcgcagct caccactgcc caactgcaaa cgtacggaga 4201 ccttagtatg atggaaatct accgtaacta ccattatgca ttcacccaac aactgatggg 4261 atgttggaac accaccaact acggcggacg ccatacgcta gacaataatg aaatgagccg 4321 tatctggaca tcgttctaca ctcaatccct gaagaatatc attgatgctc aatatcgcac 4381 agccgaagat gcagagaaag tgaatatcaa ctctgtactc cgcatttatc gggtttatct 4441 tatgtctatc atcaccgaca cttacggaga cgctcctttc agtgaagccg gcctgggatt 4501 cctcgaagga aagttcaatc cgaagtatga caaacaggaa gatatttaca acgctttctt 4561 tctggagctg gaagatgccg tcaataaaat cgatccgacc aaagataaag tgacaggtga 4621 cctcatctat gccggtgatg taactaaatg gcaacaactg gcaaactcgc ttcgcctacg 4681 ttttgccatg cgaatatcca gtgtcaatcc tacaaaggca caaacggaat ttgaaaacgc 4741 tttggctgcc aatgggggtg tgataacgga cgcctcaagc gatgcgctca tcaaatacat 4801 gacaatcgca tttagtttcg gacaagaagc ttactctgat tatcgcggaa attcattgtc 4861 acagttgttg ttcggcaacg accccgctaa taatccaagt tacctctgtt ccactttctt 4921 taatcagtta tacaattccg gtgacccccg tacattcaaa atttcccgtt gttattatga 4981 cgggctgatg agtgccacca gtcccgacaa tcgtgtcgac atcacccaag agatgattga 5041 aaaagggatt gctttcagtc cgcgcgaccc aggtgcctac tcgtgggaac catggcctac 5101 agggtacgac agcgatattt gtgcggaatt agccgttaac aacccttccg ttacagcaac 5161 catggctcgt gaagtggaac ccaaacttgc caataatttc ctaaaaagcg acaatccggg 5221 cgtagtgatg acctctgcag aagtgaagtt cctgatggcg gaagctaccg tgaaaaaatg 5281 gaatgtcggc agtgtgtcag cagaagatct ttacaagcaa ggagtacgtg ctgctattga 5341 tttcctgaca gacaactacg gttgcacggc tactactgat gcagagtttg atgcattcat 5401 tcaagataaa ggggcgttcg gacatacgga caatcagaaa cttgaagcta tcaatacaca 5461 ggcctggatt cttcacttca ccaatcctgc cgaatgttgg gcaaacgtgc gtcgttccgg 5521 ttatccgaag ttgaaatcac cggcagaata tgggttcgga caatacctca ccggtggaac 5581 ggaaattccg gtacgtctat gctatcctgt attggaatct tcctataata aaaagagcta 5641 caatgaagcc atcgagcgca tgggaggaac agacaattgg cacagccttc tgtggtggga 5701 tactgaaaac taactattaa acaatcatcc attatgaaaa agatatatat tgcactattt 5761 gccctactcg ctgtaggctc cacatttact gcatgcaccg aggaagaacc gttcgctacg 5821 gtgacagaaa atgatgatcc gcatatcctt gccccggtat tccctgacag aacgaacggc 5881 caattggcta cattcgccaa catcagtcgg gatgcgaact tatccatcgc gctgaccgtg 5941 actccgaaag attacaccac agtaacctgg ttcattgatg ggcaggaagt agaatcgggt 6001 accgactccg acaaagaaat caaccgtagt ctgaaagccg gtacgtataa tttgaaaata 6061 gaagtggaaa ctgtgaaagg gaagaagact tcccgtgaag gattggtggt ggtcaatccg 6121 ttggctgatg accctcaatc gaaagaagta gcctttgaga gaattgtatc tcccggtaag 6181 actgcccgtt tatatggcag taatttacag aacgtaacag ccatcctctt gggaggaaac 6241 acgatcaccg atcctactta tgttgaatcg gcagacgaga attatctgga atacaccatt 6301 ccgacaggcg taagcgaagg cgattaccgt atcgttctgc aagatgccga cggcaatcaa 6361 tatggagccg acatggtgaa agtaaccaat gcttcccttg tcatttcggg agctaaccgg 6421 gctactgcca acgtggattg gaccatttcc ggcatcaatc tcgaaaacat cgcttctcta 6481 accattggcg ggcaaacggt atctcaattc agcaaccaat cttctacaga gattacgctg 6541 acttgtccgg atttgtctga tggaagctat acgatgacag gcaagacccg cagcggagag 6601 gctgtgcagt tcttgaacga taatattacg actaccgaac agactgtgac cgtatctacc 6661 gaaataacgc tttggtcagg acaccattac gtttcgtggg ataagccgga cggtgatccc 6721 aacaaaactt ttggtttgat tccaatggat gtatttgctg gtatcacagc cgggtcgaca 6781 ctgaaagtag tctactccat agaacctact gccgaatatc ataaaatgca gcttgccaca 6841 ggctattgga caggcttggc aagtgagatg gaatttacgg aaaacggtga atatacactg 6901 atactaacac aagacatgtt gaataagatt caagctgaag ccggattctt atgtgtagga 6961 catggctatt atgtagattt ggtaaccgtg aaataatgtt cctaagtaag tttaaaaatc 7021 attcatacca taagtatctg tcattcttcg ggttaaagcc ctctgaatga cagattacat 7081 aaacgatgct acttaaaaac aaatcataat gaaactaaga aatattatat taatgaccgc 7141 atctatcgca atgactgcct gttccaaaca ggcagttccc actgccattc ctgaagacgc 7201 taaaatagaa caacaagtgg aagagcttct atcaaagatg gatcttgacg ccaagatagg 7261 gcaaatgacc gagcttgcta ttgatgtatt aggagagacg ataaatggag aattccaatt 7321 ggacgaggca aaactccaca aagcgattgc tgaatacaag gtaggttcgt ttctcaatgc 7381 tcccggcccg gtagcacaaa gccctgaaaa gtggaacgag attatcggtc gaatacaaga 7441 actgtcgatg gcggagattg gtattccatg tatctacggc ctggatcaga atcatggtac 7501 tacctataca ttgggcggca cgctctttcc tcaaaatatc aatatgggtg ctgccttcaa 7561 tccggatttg acctacgaag cggcacgtgt aacggcttat gaaacaaggg ctagcaattg 7621 tccctggaca tactccccca ctgtcgatat ggcacgtgat ccccgttggc cgcgtgtatg 7681 ggaaaattat ggagaagact gtttagtcaa tgccattatg ggcagcactg ctgtccgagg 7741 cttccaagga gatgatccta accatattcc cgccgaccga atcgccacct ccgtgaaaca 7801 ttacatggga tacagcatgc cacgtaccgg caaggaccgc acaccggctt atatatctgt 7861 ttccgaactg cgtgaaaaat gttttgcacc gttcagggct tgtgtggaag caggtgcact 7921 gactataatg gtcaacagtg gttccatcaa cggaaagcct gtgcacgccg accgcgaact 7981 ccttacacaa tggctgaaag aagacttagg atgggacgga atgttaatca ccgactgggc 8041 ggacatcaac aatctttata cccgcgaaca tgtggcagca aacaagaaag aagccatcga 8101 aatggcaatc aatgcaggta tagacatggc tatggaaccg tatgatctca attattgtac 8161 actactaaaa gagttagtgc aagagaagaa aatacccatg agccgtattg acgatgccgt 8221 ccgccgggtg cttagactta aattccgtct gggtctgttc gatcatccga acactttatt 8281 gaaagattat cctctcttcg gcagcaagga gcatgcactt atcgcacttc atgcagctga 8341 agaatcggaa gtattgctaa aaaataaaga taatattctt ccgcttcctc aagggaaaaa 8401 gctactggta accggtccga acgccaactc tatgcgttgc ttgaacggcg gttggagcta 8461 ttcgtggcaa ggacatctga ccgatagatt tgccgacaaa tataacacca tttacgaagc 8521 aatctgcaac aaattcggag ccgatcatgt ccgtctggaa cagggcgtaa cctacaaacc 8581 cgaaggtgct tacatggaag aaaatgaacc ggagattgag aaagctgtgg ccgccgcccg 8641 caacgtggat attattatcg cctgcatcgg tgagaattca tattgcgaaa cacccggtaa 8701 tctttccgaa cttgccatct ctgccagcca aagcaaactg gtaaaggcac ttgccgctac 8761 aggaaaaccg atcatcctga tactcaatga gggtcgtccc cgcattatca acgagttgga 8821 gccattggct gatgcagtta ttgacattct attacccgga aactatggtg gcgacgcatt 8881 ggcgaacatt ctggcaggag acgtgaaccc cagtgccaag atgccttaca catatccgcg 8941 acacgaagca gcactgacta cctacgatta tcgggtgagt gaagaaatgg acaaaatgga 9001 aggagcttat gactacaatg cagtagtctc cgttcaatgg cctttcggat acggattgag 9061 ttacaccact ttcgagtaca gcaacttcca aacggacaaa tcttcgttta ccgcaggaga 9121 tgatttgcac ttttccatag atgtaaccaa taccggtaaa tatgccggaa aagaagttgt 9181 catgctattc agcagtgatc ttgtggcttc gcttactccc gaaaaccgcc ggttacgagc 9241 cttcaaaaag gtggaactgc aaccgggcga aacccagact atcactctat ccatcaaagg 9301 cagcgacttg gcttttgtca attccgacgg gcaatgggta ctggaacaag gggatttccg 9361 tatgcaatgc ggaaatcaaa cactgaatat tatatgcaag taa //