SUMMARISING RUN PARAMETERS ========================== Input filename: UT30.3_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.8 Python version: could not detect Number of cores used for trimming: 32 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.8 with Python 3.9.19 Command line parameters: -j 32 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC UT30.3_1.fastq.gz Processing single-end reads on 32 cores ... Finished in 1240.976 s (2.699 µs/read; 22.23 M reads/minute). === Summary === Total reads processed: 459,767,358 Reads with adapters: 162,787,030 (35.4%) Reads written (passing filters): 459,767,358 (100.0%) Total basepairs processed: 58,851,774,576 bp Quality-trimmed: 110,580,464 bp (0.2%) Total written (filtered): 58,459,027,953 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 162787030 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.1% C: 24.9% G: 17.1% T: 20.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 119223474 114941839.5 0 119223474 2 30471504 28735459.9 0 30471504 3 7369230 7183865.0 0 7369230 4 2411612 1795966.2 0 2411612 5 332847 448991.6 0 332847 6 122024 112247.9 0 122024 7 46341 28062.0 0 46341 8 31303 7015.5 0 31303 9 45463 1753.9 0 30607 14856 10 134593 438.5 1 107783 26810 11 59506 109.6 1 41935 17571 12 985006 27.4 1 934569 50437 13 138481 6.9 1 123507 14974 14 332739 6.9 1 312535 20204 15 267261 6.9 1 252588 14673 16 58045 6.9 1 50946 7099 17 14082 6.9 1 8240 5842 18 12438 6.9 1 6690 5748 19 11390 6.9 1 5961 5429 20 10497 6.9 1 5051 5446 21 11325 6.9 1 5587 5738 22 11684 6.9 1 5321 6363 23 10550 6.9 1 5051 5499 24 16261 6.9 1 5314 10947 25 12070 6.9 1 5889 6181 26 13873 6.9 1 7081 6792 27 13504 6.9 1 6849 6655 28 11381 6.9 1 6015 5366 29 10890 6.9 1 5866 5024 30 10229 6.9 1 5164 5065 31 9841 6.9 1 4923 4918 32 12260 6.9 1 4549 7711 33 9410 6.9 1 4424 4986 34 9341 6.9 1 4631 4710 35 9204 6.9 1 4517 4687 36 8859 6.9 1 4288 4571 37 9627 6.9 1 4830 4797 38 9920 6.9 1 5075 4845 39 9059 6.9 1 4423 4636 40 10487 6.9 1 5525 4962 41 10280 6.9 1 5229 5051 42 9471 6.9 1 4851 4620 43 8712 6.9 1 4004 4708 44 11436 6.9 1 6277 5159 45 6348 6.9 1 2712 3636 46 7425 6.9 1 3359 4066 47 8112 6.9 1 3790 4322 48 8269 6.9 1 3963 4306 49 8473 6.9 1 4009 4464 50 8168 6.9 1 3855 4313 51 7863 6.9 1 3693 4170 52 8067 6.9 1 3856 4211 53 8542 6.9 1 4074 4468 54 9010 6.9 1 4510 4500 55 7920 6.9 1 3685 4235 56 7822 6.9 1 3617 4205 57 7748 6.9 1 3554 4194 58 7690 6.9 1 3536 4154 59 7798 6.9 1 3708 4090 60 7938 6.9 1 3858 4080 61 7911 6.9 1 3623 4288 62 9382 6.9 1 4864 4518 63 9374 6.9 1 4833 4541 64 9390 6.9 1 4781 4609 65 11289 6.9 1 6329 4960 66 8848 6.9 1 4399 4449 67 9089 6.9 1 4484 4605 68 10709 6.9 1 5456 5253 69 13175 6.9 1 6810 6365 70 43433 6.9 1 12631 30802 71 59290 6.9 1 22528 36762 72 17692 6.9 1 7487 10205 73 6194 6.9 1 2531 3663 74 3613 6.9 1 1474 2139 75 2929 6.9 1 1163 1766 76 2619 6.9 1 1001 1618 77 2589 6.9 1 1047 1542 78 2701 6.9 1 973 1728 79 2905 6.9 1 1004 1901 80 2937 6.9 1 963 1974 81 2968 6.9 1 998 1970 82 2777 6.9 1 947 1830 83 2891 6.9 1 1104 1787 84 2557 6.9 1 979 1578 85 2268 6.9 1 809 1459 86 2073 6.9 1 695 1378 87 2041 6.9 1 726 1315 88 1939 6.9 1 651 1288 89 1834 6.9 1 635 1199 90 1905 6.9 1 612 1293 91 1799 6.9 1 584 1215 92 1801 6.9 1 565 1236 93 1705 6.9 1 527 1178 94 1724 6.9 1 505 1219 95 1518 6.9 1 446 1072 96 1560 6.9 1 469 1091 97 1486 6.9 1 440 1046 98 1491 6.9 1 453 1038 99 1495 6.9 1 452 1043 100 1341 6.9 1 414 927 101 1423 6.9 1 396 1027 102 1337 6.9 1 402 935 103 1351 6.9 1 342 1009 104 1245 6.9 1 313 932 105 1214 6.9 1 286 928 106 1103 6.9 1 285 818 107 1103 6.9 1 305 798 108 1031 6.9 1 278 753 109 1024 6.9 1 266 758 110 1000 6.9 1 262 738 111 1047 6.9 1 241 806 112 978 6.9 1 247 731 113 1008 6.9 1 274 734 114 946 6.9 1 232 714 115 1003 6.9 1 270 733 116 899 6.9 1 296 603 117 896 6.9 1 239 657 118 883 6.9 1 240 643 119 900 6.9 1 275 625 120 834 6.9 1 227 607 121 772 6.9 1 221 551 122 857 6.9 1 294 563 123 843 6.9 1 278 565 124 807 6.9 1 292 515 125 831 6.9 1 322 509 126 770 6.9 1 250 520 127 656 6.9 1 229 427 128 574 6.9 1 184 390 129 528 6.9 1 147 381 130 469 6.9 1 135 334 131 439 6.9 1 117 322 132 404 6.9 1 102 302 133 369 6.9 1 87 282 134 376 6.9 1 85 291 135 400 6.9 1 101 299 136 392 6.9 1 107 285 137 370 6.9 1 93 277 138 505 6.9 1 198 307 139 315 6.9 1 62 253 140 340 6.9 1 98 242 141 295 6.9 1 73 222 142 304 6.9 1 75 229 143 312 6.9 1 63 249 144 324 6.9 1 80 244 145 337 6.9 1 88 249 146 320 6.9 1 81 239 147 350 6.9 1 101 249 148 336 6.9 1 122 214 149 628 6.9 1 289 339 150 11637 6.9 1 8459 3178 RUN STATISTICS FOR INPUT FILE: UT30.3_1.fastq.gz ============================================= 459767358 sequences processed in total