##gff-version 3
##sequence-region AAXF02000051 1 8537
# conversion-by bp_genbank2gff3.pl
# organism Bacteroides ovatus ATCC 8483
# Note Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont521, whole genome shotgun sequence.
# date 04-AUG-2012
AAXF02000051	GenBank	region	1	8537	.	+	1	ID=AAXF02000051;Dbxref=BioProject:PRJNA18191,ATCC:8483,taxon:411476;Name=AAXF02000051;Note=Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont521%2C whole genome shotgun sequence.,Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans,it represents,on average,0.034%25 of all 16S rDNA sequences and 0.071%25 of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally,PCAP (Huang,et al,Genome Research,13:2164,(2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive,sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine,the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were blasted against NCBI'snon-redundant (NR) database and predictions generated based on proteinalignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the contig level and may notnecessarily reflect any known order or orientation betweencontigs. For answers to your questions regarding this assembly or project,or any other GSC genome project,please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in August 2007,and the CDS comments were updated in January 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. ;comment1=Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans%2C it represents%2C on average%2C 0.034%25 of all 16S rDNA sequences and 0.071%25 of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally%2C PCAP (Huang%2C et al%2C Genome Research%2C 13:2164%2C (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive%2C sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine%2C the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were blasted against NCBI'snon-redundant (NR) database and predictions generated based on proteinalignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the contig level and may notnecessarily reflect any known order or orientation betweencontigs. For answers to your questions regarding this assembly or project%2C or any other GSC genome project%2C please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in August 2007%2C and the CDS comments were updated in January 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. ;date=04-AUG-2012;mol_type=genomic DNA;organism=Bacteroides ovatus ATCC 8483;strain=ATCC 8483;type_material=type strain of Bacteroides ovatus
AAXF02000051	GenBank	gene	1	1404	.	-	1	ID=BACOVA_03379;Name=BACOVA_03379
AAXF02000051	GenBank	mRNA	1	1404	.	-	1	ID=BACOVA_03379.t01;Parent=BACOVA_03379
AAXF02000051	GenBank	CDS	1	1404	.	-	1	ID=BACOVA_03379.p01;Parent=BACOVA_03379.t01;Name=BACOVA_03379;Note=KEGG: bme:BMEI1837 0.00026 cellobiose-phosphorylase K00702%3B COG: COG5368 Uncharacterized protein conserved in bacteria;codon_start=1;inference=similar to AA sequence:REFSEQ:NP_812478.1;product=hypothetical protein;protein_id=EDO10747.1;transl_table=11;translation=length.467
AAXF02000051	GenBank	exon	1	1404	.	-	1	Parent=BACOVA_03379.t01
AAXF02000051	GenBank	gene	1423	3762	.	-	1	ID=BACOVA_03380;Name=BACOVA_03380
AAXF02000051	GenBank	mRNA	1423	3762	.	-	1	ID=BACOVA_03380.t01;Parent=BACOVA_03380
AAXF02000051	GenBank	CDS	1423	3762	.	-	1	ID=BACOVA_03380.p01;Parent=BACOVA_03380.t01;Dbxref=InterPro:IPR001764,InterPro:IPR002772;Name=BACOVA_03380;Note=KEGG: bth:BT3567 0. beta-glucosidase K05349%3B COG: COG1472 Beta-glucosidase-related glycosidases%3B Psort location: Periplasmic%2C score:9.44;codon_start=1;inference=protein motif:FPrintScan:IPR001764,protein motif:HMMPfam:IPR001764,protein motif:HMMPfam:IPR002772,protein motif:ScanRegExp:IPR001764;product=glycosyl hydrolase family 3 N-terminal domain protein;protein_id=EDO10748.1;transl_table=11;translation=length.779
AAXF02000051	GenBank	exon	1423	3762	.	-	1	Parent=BACOVA_03380.t01
AAXF02000051	GenBank	gene	3875	5407	.	-	1	ID=BACOVA_03381;Name=BACOVA_03381
AAXF02000051	GenBank	mRNA	3875	5407	.	-	1	ID=BACOVA_03381.t01;Parent=BACOVA_03381
AAXF02000051	GenBank	CDS	3875	5407	.	-	1	ID=BACOVA_03381.p01;Parent=BACOVA_03381.t01;Dbxref=InterPro:IPR012944;Name=BACOVA_03381;Note=COG: NOG26302 non supervised orthologous group%3B Psort location: OuterMembrane%2C score:9.52;codon_start=1;inference=protein motif:HMMPfam:IPR012944,similar to AA sequence:INSD:AAO78674.1;product=SusD family protein;protein_id=EDO10749.1;transl_table=11;translation=length.510
AAXF02000051	GenBank	exon	3875	5407	.	-	1	Parent=BACOVA_03381.t01
AAXF02000051	GenBank	gene	5472	8537	.	-	1	ID=BACOVA_03382;Name=BACOVA_03382
AAXF02000051	GenBank	mRNA	5472	8537	.	-	1	ID=BACOVA_03382.t01;Parent=BACOVA_03382
AAXF02000051	GenBank	CDS	5472	8537	.	-	1	ID=BACOVA_03382.p01;Parent=BACOVA_03382.t01;Dbxref=InterPro:IPR000531,InterPro:IPR008969,InterPro:IPR012910;Name=BACOVA_03382;Note=COG: NOG26156 non supervised orthologous group%3B Psort location: OuterMembrane%2C score:9.52;codon_start=1;inference=protein motif:HMMPfam:IPR000531,protein motif:HMMPfam:IPR012910,protein motif:superfamily:IPR008969;product=TonB-linked outer membrane protein%2C SusC/RagA family;protein_id=EDO10750.1;transl_table=11;translation=length.1021
AAXF02000051	GenBank	exon	5472	8537	.	-	1	Parent=BACOVA_03382.t01