LOCUS NC_013132 13159 bp DNA linear CON 26-AUG-2019 DEFINITION Chitinophaga pinensis DSM 2588, complete genome. ACCESSION NC_013132 VERSION NC_013132.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN00001914 Assembly: GCF_000024005.1 KEYWORDS RefSeq. SOURCE Chitinophaga pinensis DSM 2588 ORGANISM Chitinophaga pinensis DSM 2588 Bacteria; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga. REFERENCE 1 (bases 1 to 13159) AUTHORS Glavina Del Rio,T., Abt,B., Spring,S., Lapidus,A., Nolan,M., Tice,H., Copeland,A., Cheng,J.F., Chen,F., Bruce,D., Goodwin,L., Pitluck,S., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Chain,P., Saunders,E., Detter,J.C., Brettin,T., Rohde,M., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lucas,S. TITLE Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034) JOURNAL Stand Genomic Sci 2 (1), 87-95 (2010) PUBMED 21304681 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 13159) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Chitinophaga pinensis DSM 2588 JOURNAL Unpublished REFERENCE 3 (bases 1 to 13159) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (12-AUG-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 08/26/2019 00:28:39 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Method :: Best-placed reference protein Annotation Software revision :: 4.9 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; Genes (total) :: 7,171 CDSs (total) :: 7,077 Genes (coding) :: 7,009 CDSs (with protein) :: 7,009 Genes (RNA) :: 94 rRNAs :: 6, 6, 6 (5S, 16S, 23S) complete rRNAs :: 6, 6, 6 (5S, 16S, 23S) tRNAs :: 73 ncRNAs :: 3 Pseudo Genes (total) :: 68 CDSs (without protein) :: 68 Pseudo Genes (ambiguous residues) :: 0 of 68 Pseudo Genes (frameshifted) :: 19 of 68 Pseudo Genes (incomplete) :: 47 of 68 Pseudo Genes (internal stop) :: 11 of 68 Pseudo Genes (multiple problems) :: 8 of 68 ##Genome-Annotation-Data-END## ##Metadata-START## Organism Display Name :: Chitinophaga pinensis UQM 2034, DSM 2588 Culture Collection ID :: DSM 2588, ATCC 43595, LMG 13176, UQM 2034 GOLD Stamp ID :: Gi02244 Funding Program :: DOE-GEBA 2007 Gene Calling Method :: Prodigal Isolation Site :: Pine litter in Australia Isolation Country :: Australia Oxygen Requirement :: Aerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 23 - 24C pH :: 7 Gram Staining :: gram- Biotic Relationship :: Free living Diseases :: None Phenotypes :: Biomass degrader, Chitin degradation Energy Source :: Chemoorganotroph ##Metadata-END## REFSEQ INFORMATION: The reference sequence was derived from CP001699. URL -- http://www.jgi.doe.gov JGI Project ID: 4082984 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline (PGAP) set; GeneMarkS-2+ repeat_region COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..13159 /organism="Chitinophaga pinensis DSM 2588" /mol_type="genomic DNA" /strain="DSM 2588" /type_material="type strain of Chitinophaga pinensis" /db_xref="taxon:485918" /note="type strain of Chitinophaga pinensis" gene complement(1..870) /locus_tag="CPIN_RS31475" /old_locus_tag="Cpin_6364" CDS complement(1..870) /locus_tag="CPIN_RS31475" /old_locus_tag="Cpin_6364" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_012793932.1" /translation="MKWIKDVNIFILSTLGVFLAAGCITQASSPSPTDSIMTDSAAVAI DSITATADMIAEPDSLQGEMYVIDGLIGLIAESSLQLSNASMPPVAGRDTLNATLYHEN GHPVEMSYTLFEDGGAAAGIGNMYFFADYPIVSELAYGDTHIYNVLRKDSTFFSFSRNG RNYFKLITLPEGAEDLRIAHLIKDAADMLQLYPRNKIRFNVPSVPLRGDLKLLAVADVP MYEWPDSTLPSILVIPENAVLGFMGANMQEVVTDGKKWIWYEVTCGGVHGWINGHPAFV QERNDEVY" gene complement(908..2647) /locus_tag="CPIN_RS31480" /old_locus_tag="Cpin_6365" CDS complement(908..2647) /locus_tag="CPIN_RS31480" /old_locus_tag="Cpin_6365" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="GH51" /protein_id="WP_012793933.1" /translation="MKRILVLFFLCSEILLCTACNKNSGEPYEGEETDTSGTTVIVPED PALASSIGFFMSSWAPKTFKAGTFTEAALPGSAPYLVTVDASTITTKISPALFGNNSNP YMSQMVNQPKLIGHLTNLRTGIIRFPGGNISSVYFWNGQVGIPPADAPVQLTDADGNKT DPGYWYGNNTADWTLSVNNYYSMLQQTGNEGMITVNYGYARYSTAANPVAAAAHLAADW VRYDNGRTRYWEIGNESNGTWQAGYRIDVTQNKDGQPQVISGELYGRHYKVFADSMRKA AAETGKTIYIGAQLLEHEPAIWATDTDKKWNAGVLQQVGATADFYIVHSYFTPYNTNSN PQEILASAATVTKAIYDHVNGSFTANSVAAKPVALTEWNIFAVGSQQMVSQVAGMHAVM VWGELIRNKFGMSSRWDLANAWENGNDHGLFSQGESLSGETKWTPRPAFYYQYFFQKFL GDRGINATVTDSNSSISAYASTFSSGEVGLTLVNRANITRNVTVNFKNFLPGARFYWYV LTGGEGVTGFSRKVFVNGAGPTGVSGGPDNYAALIPYGGSATGGVKVSLPPMSVVNMVI EKK" gene complement(2723..3982) /locus_tag="CPIN_RS31485" /old_locus_tag="Cpin_6366" CDS complement(2723..3982) /locus_tag="CPIN_RS31485" /old_locus_tag="Cpin_6366" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_012793934.1" /translation="MTRNIFTFIRYMNRLPLYLLILFTVFCAACSKDDGSSGAPPVVTG VSLLDASKTDSTFAGTLPGTLILITGQNLDGIVKIAFNGQAAYFNPTYNTRTHLIVTIP DNAPTEATDPDVPNTIHIITSHGETSYSFTLEIPPPSIVAISNENALAGDSLIIYGSSL WLIDKIVFPGGREVTDFIADDAGTRVGMIMPALGDDTGRLVIQAKYGTAVSDGPLNDHE SGNVISNLTADGETGEKAVFNWAYWGANRTTDATLFPGTRGGYLQNIFGGVGVNDGAWW NGNRSGNFNDVPMFTAAIMTEQASSYALKFEISTKEPWTAGVNVLRFGDNYAYRYMPWS GAAGAKFETKGAWQTITVPLSAFKTVADGKEGTGAGAQTMGDLLKAGGVVAFGYRFITE DAPVDVYNAAYDNFRIVKIR" gene complement(3999..5642) /locus_tag="CPIN_RS31490" /old_locus_tag="Cpin_6367" CDS complement(3999..5642) /locus_tag="CPIN_RS31490" /old_locus_tag="Cpin_6367" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009279815.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="RagB/SusD family nutrient uptake outer membrane protein" /protein_id="WP_012793935.1" /translation="MKKTNYHIYLIALACTLFAAGCSKDFLNRPPEDAIVDVNFYQTTD QILAGTAPLYNIVWFAYNDKASHGIGDGRAGILMSGSYQVDNIRMQTTDVTPEVYSSWT SFFNVVAQANQIISNLVTYTAPTVPENVKQAGIAEGRFMRGVAYSYLVQNWGAVPIIAD NKVLLTDTSISRNTIASVWEFIIRDIRFAAENLPAAPMQKGRLTKWAAEGMLAKMYLTR AGISGSKNQTDLDSAAWYANDVIMNSGAALMDNYEDLFLVKNNNNSESLFALQWKYDGD WGTQNSVQAFLAYGSEITGFGDGWGGDLGASLDQLKLYRIDDKRRKGTFMFPGDHYSYI HQSVDDPNNPGKKIIQELQVPWNYVGTERYNARAWVKKYVVGRPEDNDGKVTQQHTEIN TYMLRLADVYLIYAEALLGKSASTTNAQALLYFNKVRKRAGVPEKTVLTWDDIYIERRL ELAMEGQAWYDLVRLYYYDPAKAISLINAQDRGTYRVAANAATNATSWTITSDVPAFYN VTQSNFLLPLPAAELTAAPNLRKPPVPYQF" gene complement(5663..8860) /locus_tag="CPIN_RS31495" /old_locus_tag="Cpin_6368" CDS complement(5663..8860) /locus_tag="CPIN_RS31495" /old_locus_tag="Cpin_6368" /inference="COORDINATES: protein motif:HMM:TIGR04056.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q45780|1.B.14.6.1" /protein_id="WP_012793936.1" /translation="MKRASITGKSYLTVWVMIFFWAVTMPAQAQTQSDPGRKISGSVTA GETGTPVPGASITIKGTKRMTTTDGAGNFSIPAAPGEVINIASMGFITREFKVGNSATV NVVMQEDYRKLNDVVVVGYGKMKKTDQSSAQVSITSTEVNRTINTTIDQAIQGRAAGVY VTQNSGQPGGGISVNIRGVNTLSGTNEPLYVIDGVQMQPATVSYGATSSVNPLAGINPS DIESLEILQGPSATAVYGSRATNGVILVTTKRGKSGQMKLSYNYLYSLQDKPEAVNTMT LQQFAIMNNEIAQLLNRTPTPEFQNPGVLGKGTNWQDALFRTASLQKHQVNISGGASNT TYYMSGEYFDQEGVAIGSDFNRYSFRLNLDNQAFKWLKLSTSLNFYQTKEKLASTSEDV IRNAINLAPNIPVKNADGSWGGATENEYGSNAKYAPLNPVAIASLVSNDYKRTGGLGGA TAEISIIKGLTFRTSFNGNFEYTDGSKFIPTYQLGYNVNEKASLAVSNGKNFYWNLNEL LQYSRDIGKHSFSVMVSHEAQAWNYQGFSAQRVGFASNSIPSLNLGNALGQTTGSYKGS GALESYLGRVNYTYDNKYIVQLAGRADGSSNFGADDRWGYFPSVSVAWRLTGEEFMKSL PFINELKIRAEYGTTGNNGSGGAQFSSLTSVTTPWGAGFRTGQYGNSGLKWESTETKNI GFNMSVLKDRIQLEGDFYIKKTDNLLMPNPLPAYMGTQGEGAINAPIVNIGAMQNKGFG ITLNTVNIDNKQGFSWRTNFNISSFKTKITKFYSDAAFLSRSAWFMNNFTSRSVIGDAP WQFYGYVAEGLFQSVDEINSSAIPAQSDGTRLPVAKDGGVWVGDIKYKDLNGDNIIDSR DQTFIGNPWPKLTFGLTNTFSWKGFDLSVLMTAAQGNDIFNYLRFENTNPNNINLGRNL LQESFNYARVGDVGGKTILENPGANVPRIVGTDVNGNGNRFTNLYVEDGSYIRIKNVQL GYNIPHALLRQQSVVKNIRLIAGVQNLATFTKYKGYDPEVGSYLGKEVQLSSQLIGVDA GRYPLTRLYSASVAVDF" gene complement(9059..13159) /locus_tag="CPIN_RS31500" /old_locus_tag="Cpin_6369" CDS complement(9059..13159) /locus_tag="CPIN_RS31500" /old_locus_tag="Cpin_6369" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014218988.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI LY|0036286,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607" /protein_id="WP_012793937.1" /translation="MLMKNLLLPLLLLVSYAYAQPSYQFSSLDVNNGLSNNQVNSIYKD ARGFMWFGTMSGLNRYDGYAFKTFRHSAEPGVINDDYITGIYPAPNNQLFIRTRNGDNF YNPVTEQFSDATTWLKKKGLSEYGITSVAQTGRNWWIAYNDARLYKTDSTGRTTKILLP DRANAAIADLQGEGDKLLVILYLNGDVEKLDIASNKIIYRNSNIRKYIGKPPLALRIFI DAAKDMWVYMPGSDFGVAYLNRTDGKVKMLSSKTGALNNDIVNSIVEDDKHFIWIGTDH GGINLIDKNNNFKSRFLVNKEGDLKTIAENAVYALYKDNFGIIWCGTYKRGLSYFTENM VKFPLYKHSHADNNSLSYNDVNCFAEDEKGNIWIGTNGGGLIYFDRVNQTFKRFQHDPS NDNSISKDVIVSLYVNNYNDLWIGYYFGGMDFYSNGRFNHYRHEEDDPNSLANKSVWKI YRDQRNNFWVGTLGGGLDRFDPGNGIFYHNNVNMPASVHSNYISGFAEDNRGNLWIATA YGIDVLDNSKGVFVHYLSSTHQLSNDNVSSLLYDKRGHMWAGTRAGLNVFDEKVQQFRS FTVKDGLPDNNIITMLEDNEGYLWASTSNGLSRIAVKDGPEGLSISCRNYDDKDGLQSK AFNVNAAFKLSTGELIFGGADGFNLFDPSSLPRNNTRPDVVFTGLRLFDRNIEVNAKVH DRVLLPEALSVTKEIVLKHNENDFSLEFAALNFINTEKNKYAYRLDQFNDNWLIADGST RRITYTNINPGTYTLQVKAANEDGFWNEEGISLKIIVKPPFWKTPLAYIIYVLLTVAIL LLLRRSVIRRAHKRFTLEQERQEARRMHEMDMMKIRLFTNLSHEFRTPVSLILSPLEEI MQQSGEQKEKGKFQLIYRNAKRLLILVNQLMDFRKMEMQELRLNPTWGDIAVFIREVTF SFADLAERKQILFSYKSNAPHVYTYFDNDKIERILFNLLSNAFKFTPSGGAVHVEVNSN KDEQEKTWLEMKVRDTGIGIPEERHEKVFEQFFQSDMPGGLMNRGSGIGLAITREFVKL NNGTITVESEVNKGSCFTVRLPLETCEQPEVADEPQEGVKDQLLTEDPLFAEELPEELS EEELATDTQRQTVLLIEDNEDFRFYLKENLKDCYHIIEGADGIAGWQKTLSGHPDLVMS DVNMSGMNGIDLCRKIKNDPRTKHIPVILVTAAAGEQEQLRGLEAGAADYLVKPFNFEI MLSRVRNLLARPLPAKHQVSLPELQNAENEASPNEKFLRRVLEIVEQNLSNAGFSVEEL SRELYMNRVSLYRRIFNLTGQTPVEFIRMIRIKRAAQLLSKTEMNVTEVAYEVGFNNPK YFAKYFKMAYNMLPSAYATAARK" ORIGIN 1 ctaataaact tcatcattcc gctcctgcac aaatgcagga tgtccattga tccatccatg 61 tacgccgcca cacgtcacct cataccagat ccatttctta ccatctgtta ccacctcctg 121 catgttcgcg cccataaatc ccagcaccgc gttttcaggg atcaccagta tcgatggtaa 181 agtagaatcc ggccattcat acatcggtac atccgctacc gccagtagtt tcagatctcc 241 tcttaaaggc acggaaggta cattaaaccg gatcttattc cgtggataca attgtaacat 301 atcagccgca tccttgatca gatgtgcgat ccgcaggtcc tccgcccctt ccggcaatgt 361 gatcagctta aaataattac gtccgtttct gctgaaggaa aagaaggtac tatcttttct 421 cagcacatta tagatatgcg tatcaccata tgccagttca ctgacgatcg gataatcggc 481 aaagaaatac atattaccta tccccgcagc agccccgcca tcttcaaaaa gcgtatagct 541 catttcaacc ggatgcccgt tttcatgata aagcgttgca ttcagcgtat ccctgccagc 601 caccggcggc atactggcat ttgacagctg taaagacgat tcagcaatca accctatcaa 661 accatcaatt acatacattt ccccctgcaa tgaatcaggc tccgctatca tatctgctgt 721 ggcagtaata ctgtcaatgg ccaccgcggc actgtcagtc atgatgctgt cagtcggaga 781 gggactactt gcctgggtga tacaaccggc agctaaaaat acacctaaag tactcaggat 841 aaatatgttg acatctttta tccatttcat gacatagagt tatattattt atttctcaaa 901 tataactcta tttcttttct atcaccatat tcaccacact cattggtggt aaagatacct 961 tcacccctcc tgtcgcactg cctccgtaag ggatcagcgc cgcataatta tccggccctc 1021 cggatacgcc cgtcggtcct gcaccattta caaatacctt ccgtgaaaaa ccagtcactc 1081 cctcccctcc tgtcaacaca taccagtaga aacgggcccc gggaagaaaa ttcttaaaat 1141 tcaccgtcac attacgtgta atatttgccc tgttcaccaa tgtcaatccc acctctccgg 1201 aactgaatgt tgacgcatag gcactgatac tcgaattact atctgttaca gttgcattaa 1261 ttcctctgtc tcccaggaat ttctgaaaga aatactggta ataaaaagcg ggtctgggcg 1321 tccattttgt ctcacctgaa agcgattctc cctgactgaa taatccatgg tcattaccgt 1381 tctcccatgc atttgccagg tcccagcgac tggacatacc aaatttatta cggatcagct 1441 ctccccatac catcaccgcg tgcatacctg ccacctgcga taccatctgc tgagaaccta 1501 cagcaaatat gttccactcc gtcagcgcaa caggttttgc cgccacgcta ttcgcagtaa 1561 aagaaccatt gacatgatca taaatagctt tcgtgacagt agcggcagaa gccagtattt 1621 cctgcggatt tgaattcgta ttataagggg tgaaataact atgaacgata taaaaatcag 1681 ctgtagcgcc tacctgttgt aatacgccgg cattccattt tttatcggta tcggtcgccc 1741 agatggccgg ctcatgctcc agcaactgag cgccgatgta aatggtcttt cctgtttctg 1801 ctgctgcctt acgcatagag tcagcaaata ccttgtaatg acggccatat aactctccgc 1861 tgatcacctg tggctgccca tccttattct gcgttacatc tattctgtaa ccggcctgcc 1921 aggtgccatt actttcatta ccgatctccc agtaacgggt acgtccgttg tcatacctta 1981 cccagtctgc cgccaggtgt gccgcagcag caaccggatt cgcagcagta ctatatctgg 2041 cgtagccata attcaccgta atcattccct cattccccgt ttgctgtaac atactgtaat 2101 agttgttgac agacaatgtc cagtcagccg tattgttacc ataccaatac ccgggatctg 2161 tcttgtttcc atctgcatct gtcagctgta caggtgcatc cgcaggcggt atacctacct 2221 gtccgttcca gaaatacaca ctgctgatat tccctccggg aaagcggata atacccgtac 2281 gcagattagt cagatgtcct atcagtttcg gctgattcac catctggctc atatagggat 2341 tgctgttatt gccaaacagc gcaggagaaa tcttcgtagt gatcgtcgat gcatccaccg 2401 taacgagata cggggcagat ccaggcaatg cggcttccgt gaaagtgcct gccttaaaag 2461 tctttggcgc ccatgacgac ataaagaagc ctatactgga cgccagtgca ggatcttcag 2521 gtactatcac cgtagtaccg gaggtgtccg tctcttctcc ctcgtatggt tcaccactgt 2581 ttttgttgca ggccgtacac aacaatattt ccgaacataa aaaaaacagg accaatatgc 2641 gcttcattct taaatacgta ttaataagtt tgtgctataa caggtccgca gtaaaactac 2701 tgcagacctg tcattgatat tactatctga tcttcactat cctgaaattg tcatatgcag 2761 cattgtagac gtctacaggt gcatcttctg taatgaagcg ataaccaaaa gctaccacac 2821 cacctgcttt caacaggtcg cccattgtct gtgcacccgc accggtacct tctttaccat 2881 ctgccactgt tttgaaagca gacaaaggca ccgttatcgt ctgccacgcg cctttcgttt 2941 caaactttgc accggcagcg cctgaccatg gcatatagcg atacgcataa ttatctccga 3001 aacgcaacac atttacacca gccgtccagg gctcttttgt gctgatctca aacttcaatg 3061 cataactgga agcctgctcc gtcatgatcg cagccgtgaa catcggtaca tcattgaagt 3121 tacccgaacg gttaccattc caccatgcgc catcattcac acctacgcca ccgaaaatgt 3181 tctgtaaata gcctcctctt gtaccgggga acaaagtggc atctgtcgtt ctgttagcgc 3241 cccagtaagc ccagttaaaa acagccttct cacctgtctc cccgtcagcc gtcagattac 3301 tgatcacatt tccgctctca tgatcattca gcggaccatc cgagacagcc gtaccatatt 3361 tcgcctgtat gaccagtctc ccggtatcat cccccagcgc cggcatgatc atccctaccc 3421 tggttcctgc atcgtctgcg ataaaatcgg ttacttctct acctccagga aacacgattt 3481 tgtcgatcag ccataagctg gaaccataaa tgataaggga atctcctgcc agcgcatttt 3541 cattcgatat ggcgacaatc gatggaggag gaatttccag ggtgaatgaa taacttgttt 3601 caccatgact ggtgatgata tggatggtat tgggtacatc cggatctgta gcctctgtag 3661 gcgcattatc cgggatcgtg actatcaggt gcgtacgggt attgtaagtg ggattaaagt 3721 acgccgcttg cccgttaaaa gctattttaa cgataccatc aaggttttgt cctgtgatca 3781 ggattaaagt accaggcaga gtcccggcga aagtactgtc tgtcttgctg gcatccagca 3841 gactgacacc tgtcacgaca ggcggcgcac cactgctgcc gtcgtccttg gaacaggcag 3901 cgcaaaacac agtgaacagg atcaataaat acaacggcaa cctgttcata taacggataa 3961 acgtgaatat attacgggtc atgatcagtc agttttaatt agaattgata aggtaccggg 4021 ggcttcctta aattaggtgc agcagtcagt tcagccgccg gcaacggcaa caggaaattg 4081 ctctgagtca cattgtagaa tgccggcaca tcagaagtga tcgtccagga agtcgcattc 4141 gtagcagcat tggcagcaac acgataagta cccctgtcct gcgcattaat cagcgaaatc 4201 gctttcgcag gatcatagta atacaacctc acgagatcat accaggcctg tccttccata 4261 gctaactcca gtcttctttc aatataaata tcatcccagg taagcaccgt cttttcaggt 4321 acacccgctc tttttctcac cttattgaag tacagcaatg cctgtgcatt agtagtagaa 4381 gcagacttcc ccagcaatgc ttccgcatag atcaggtata catccgcaag tcgtaacata 4441 taggtattaa tctctgtatg ctgctgcgtc accttaccat cattatcttc cggacggccc 4501 accacatatt tcttcaccca tgcccttgca ttgtatcgct ccgtacccac gtagttccag 4561 ggcacctgca attcctgtat gatcttcttg ccgggattat tgggatcatc taccgactga 4621 tggatatagg aataatgatc accagggaac atgaacgttc ccttacgacg cttatcatct 4681 atcctgtata atttcagctg atctaaggat gccccgagat ctcctcccca accatcaccg 4741 aatcctgtta tttcggaacc ataggccagg aatgcctgca cactgttctg tgtcccccag 4801 tcgccatcat acttccattg taaggcaaac aggctctctg aattattgtt gttcttcacc 4861 aggaagaggt cttcgtaatt atccatcaat gccgcaccac tgttcataat cacatcattg 4921 gcataccagg cggcactgtc cagatctgtc tgattctttg aaccggaaat accggcgcgt 4981 gtcaggtaca ttttcgccag catcccttcc gccgcccact ttgtcagacg ccccttctgc 5041 atcggtgcag ccggaagatt ttctgcggca aaacggatgt cacggatgat aaattcccat 5101 acagatgcta tagtattacg gctgatagaa gtatctgtca gcaatacttt attatctgcg 5161 atgatcggca ctgctcccca gttctgtacc aggtaagaat aagccacacc acgcataaaa 5221 cggccttctg caatacctgc ctgtttcaca ttctcaggta ccgtcggcgc tgtataagta 5281 accaggttgc tgataatctg attggcctgt gccaccacat tgaaaaaaga agtccaggaa 5341 ctatatactt ccggtgtaac atccgtcgtc tgcatacgga tattatccac ctggtaggaa 5401 cccgacatca atatgcccgc tctgccatct ccgataccat gagaagcttt atcgttataa 5461 gcaaaccata caatgttgta cagcggggcc gttccggcga gtatctgatc agtcgtctga 5521 tagaagttca catctacgat agcgtcttcc ggtggcctgt tcaggaagtc tttactacaa 5581 cctgcggcga aaagcgtaca ggcaagggcg atcagatata tatgatagtt tgtctttttc 5641 atactgtcag ttttaaagct cattaaaaat caaccgccac acttgcgcta tacaagcggg 5701 tcaatggata gcgaccggca tccacaccaa tcagctgact gctcagctgc acttccttac 5761 cgagataaga acctacttca ggatcgtatc ctttgtattt ggtgaaagtg gccaggttct 5821 gtacacctgc gatcaggcgg atgtttttaa cgacagactg ttgtctcagt aatgcatgtg 5881 gaatgttata tcctaactgt acattcttaa tgcggatata agatccgtct tctacataga 5941 ggttcgtaaa acggttgccg ttaccgttta catctgtgcc tacaatcctg ggtacattgg 6001 cgccgggatt ctctaagatg gtcttaccac ctacatctcc gacgcgtgca taattaaatg 6061 attcctgtaa caagttccgg ccgagattga tattattcgg attcgtattc tcaaaacgga 6121 gataattgaa gatgtcattg ccctgtgcag cggtcatcaa tacactcagg tcaaatcctt 6181 tccatgaaaa agtatttgtc aaaccaaaag tcagctttgg ccaggggtta ccgataaatg 6241 tctgatcacg tgaatcgatg atattatccc cattcaggtc tttgtatttg atatcgccca 6301 cccatacgcc accgtcttta gccacaggta aacgggtacc gtcagattgt gcaggaatag 6361 cgctgctgtt gatttcatcg acagactgaa acagtccttc tgcgacataa ccatagaact 6421 gccagggcgc atcgccgatc acagaacggg aggtaaagtt attcatgaac caggcagacc 6481 ttgacaggaa agcagcatca gaatagaact tcgtgatctt tgttttgaaa gaagagatat 6541 tgaaattggt cctccagctg aaaccctgtt tgttatctat attaaccgta ttcagtgtaa 6601 taccaaaacc tttgttctgc atcgccccga tatttacaat tggagcattg atagcgcctt 6661 ctccctgtgt acccatatag gcaggcaatg gattaggcat cagcaggttg tcagttttct 6721 tgatatagaa atcaccttcc agttgtatac ggtcttttaa tacactcatg ttaaaaccga 6781 tgttctttgt ttcggtggat tcccatttga gtccggagtt accatactgt ccggtacgga 6841 aacctgcgcc ccatggcgta gtcacagaag tcagggaaga gaactgtgca ccaccgctac 6901 cattgttacc ggtcgtacca tattctgcac ggatcttcag ttcgttgata aagggtaatg 6961 atttcataaa ttcctcccct gtcagacgcc aggctaccga tacagaagga aaatatcccc 7021 atctgtcatc tgcaccaaag ttggaggaac catctgctct gccagccagt tggacgatat 7081 acttgttatc gtacgtgtag ttaacacggc cgagataaga ttcaagtgca cccgatcctt 7141 tgtaactacc cgttgtttgt cctaaggcat tacccaggtt gagagaaggg atactgttgc 7201 tggcgaatcc tacacgctgt gcagagaaac cctgatagtt ccatgcctgt gcctcgtggc 7261 tcaccatcac actgaaacta tgcttaccga tatcacggga gtattgcagt aattcgttca 7321 ggttccagta gaagttcttt ccgttggata ctgcgagcga agctttctca ttgacattat 7381 aacctaactg ataggtagga ataaacttgc tgccatctgt atattcgaaa ttcccgttaa 7441 agctggtgcg gaaggtcagt ccttttatga tcgagatttc ggcagtagcg ccgcccagtc 7501 caccggtacg tttataatca ttgctcacaa gactggcgat tgcaacagga ttcagcggcg 7561 catatttcgc attggaacca tactcatttt ccgtagcacc gccccagctg ccatctgcat 7621 ttttcacggg aatattaggg gccaggttaa tggcatttct gattacgtct tcactggtag 7681 acgccagttt ttccttcgtc tgatagaagt tcagggacgt actcagcttc agccatttga 7741 aagcctggtt atccaggttc aggcggaaag agtaacggtt gaaatcagaa ccgattgcca 7801 ccccttcctg gtcaaaatac tcgcctgaca tataataggt ggtattgctg gcgcctccgc 7861 tgatattcac ctggtgcttc tgtaatgaag cagtacggaa cagggcatcc tgccagttcg 7921 tccccttccc taacacaccg ggattctgaa attccggtgt aggggtacgg ttcagcaact 7981 gggctatttc attgttcatg atggcaaact gttgcaatgt catggtattg actgcctccg 8041 gtttgtcctg cagcgaatag agatagttat aagacagttt catctgtccg gacttccctc 8101 ttttggtagt caccaggatc accccattgg tggcccggga accatatacc gcagtggcag 8161 aaggtccttg cagtatctct aaggactcta tatccgaagg attgatccct gccagcggat 8221 taacagaact ggtcgctcca taagacaccg tagccggttg catctgtacc ccatcgatga 8281 catacagcgg ttcattggta ccgctgaggg tattaacacc acggatattc acggagatac 8341 caccacccgg ttgcccggag ttttgtgtaa catacacacc ggcggcacgg ccctgtatag 8401 cctggtcgat ggtggtattg atggtacggt tgacttctgt ggaggtaatg gagacctgcg 8461 cactgctctg gtctgttttc ttcattttac catacccgac gaccaccacg tcattcagtt 8521 tgcggtaatc ttcctgcatc actacattca cagtggcgga attacctacc ttgaattccc 8581 gggtaatgaa acccatggag gcaatattaa ttacctctcc cggagcagcc gggatactaa 8641 aattccctgc cccgtcagtt gtggtcattc tttttgtccc tttaatagta attgaagccc 8701 ccggaacagg tgtgcctgtt tctccggcgg taacgctgcc ggagatcttt cgtcccgggt 8761 cagactgggt ttgtgcttgc gcaggcatag tgacagccca aaagaaaatc attacccata 8821 ccgtcaggta cgattttcca gtaatactgg ctcgtttcat agcgtggaag aattaagttt 8881 ttagattaat gcgcgattcg tttgattttg gtcatcattg ctcatatcgt gttccccttg 8941 taggataaaa gtatcatccc tccgggagaa agagggctgt agaatgttta ttaaatagga 9001 taaaatgttg caggaggggt atcaaatgtt gctttacggg catcatttat tgcaatacct 9061 actttcgggc cgcggttgca taggcagagg gcagcatatt ataagccatt ttgaagtact 9121 tggcgaagta tttcgggttg ttgaatccta cctcataggc gacctcggta acgttcattt 9181 cggttttaga taataactgt gcagcccttt tgatacggat catacgtata aactctaccg 9241 gcgtctgacc ggtcaggttg aaaatacgac gatacaggga gacccggttc atataaagtt 9301 cccggctaag ttcttctacg gagaacccgg cattggacag attctgttct actatttcca 9361 gcacccgacg gaggaacttc tcattagggg atgcctcatt ttcagcgttt tgcagttccg 9421 gcagactcac ctgatgtttc gccggcaatg gtctggccag cagattacgt accctggata 9481 acatgatctc aaaattgaaa ggttttacca ggtaatcggc tgccccggct tccagtccgc 9541 ggagctgttc ctgttctccg gcagctgccg ttaccaggat gacagggata tgtttggtcc 9601 gcgggtcatt tttgatcttc ctgcacaggt caattccgtt catgcccgac atattcacat 9661 cactcatgac cagatccgga tgccctgaca gggttttctg ccagccggca ataccatctg 9721 ctccttctat aatatgataa cagtctttca ggttctcctt caggtaaaaa cggaagtctt 9781 cattgtcttc tatcaggagg acggtttgcc gttgggtatc ggtggccagc tcctcttctg 9841 acagttcttc cggcagctct tccgcgaaca ggggatcttc tgtcaatagc tggtctttta 9901 cgccttcctg cggctcgtct gccacctccg gctgttcaca ggtctctaaa ggcagtctta 9961 ccgtaaaaca gctgccttta ttcacctcgc tctctacggt gatcgttcca ttattcagtt 10021 tcacaaattc acgggtaata gctagtccga taccactccc cctgttcatc aaccctcccg 10081 gcatatcact ctggaaaaac tgttcaaata ccttttcatg tctttcctca gggatgccga 10141 taccggtatc tcttactttc atttccagcc aggtcttctc ttgttcatct ttattactgt 10201 ttacctccac atgcaccgct ccgccgcttg gcgtaaactt aaaagcattg gacaggagat 10261 taaacaggat cctttctatt ttatcattgt cgaaataggt atagacatgc ggagcattgc 10321 ttttatagga gaagaggatc tgtttccttt ctgccagatc agcgaatgaa aaagttactt 10381 cccggataaa cacagctata tcgccccagg tagggtttaa acgcagctcc tgcatttcca 10441 tcttccggaa atccattagt tgattcacca gtatcaataa tcgttttgca ttccggtaga 10501 tcagctggaa cttacctttc tctttttgtt caccgctttg ctgcataatc tcttccagtg 10561 gtgacaggat cagggagacc ggtgttctga actcatgact aaggtttgta aacagacgga 10621 tcttcatcat atccatttca tgcatacgcc tggcttcctg tctttcctgt tccagtgtaa 10681 agcgcttatg cgcccttctg atcacactac ggcgcagcag taataatatg gccacggtca 10741 aaagtacata gatgatatag gccagcggcg ttttccagaa aggcggtttt acaataatct 10801 tcagtgaaat gccctcttca ttccaaaaac catcttcatt ggccgccttc acctgtaagg 10861 tataagtacc gggattgata ttggtatagg taatacgccg ggtgctgcca tctgcgatca 10921 accagttatc attaaactga tccaggcgat acgcatattt attcttctcc gtattgataa 10981 aattcagtgc ggcaaattca agcgaaaaat cgttttcatt atgtttaagc acaatttctt 11041 tggtgacaga taaggcttcc ggcaacaata ccctgtcatg cacttttgca ttgacttcaa 11101 tattacggtc gaataaacgg agtccggtaa ataccacatc aggacgtgta ttattccgtg 11161 gcagagaaga aggatcaaac aggttaaacc catcagcacc accgaagata agttcgccgg 11221 tactgagttt aaatgccgca ttcacattga atgccttgct ttgcaaaccg tctttatcat 11281 cgtaattccg acagctgatg ctgagtcctt ccgggccatc ctttaccgcg atccggctaa 11341 gtccgttgga agtactcgcc catagatacc cttcattatc ctccagcatg gtgatgatgt 11401 tattatccgg cagaccgtcc ttcaccgtaa acgatctgaa ttgctgtact ttttcatcaa 11461 atacattcag accggcacgg gtacctgccc acatatgccc tcgtttgtca tagagcagcg 11521 aactgacatt atcattactc agttgatggg tggaactgag atagtgtaca aagacgcctt 11581 ttgaattatc cagcacgtca ataccatagg cagtggcgat ccacaggttc cccctgttgt 11641 cttctgcgaa tccggagata taattggaat gaacggatgc cggcatattg acattgttgt 11701 gatagaagat gccattacca ggatcaaacc ggtccagtcc tccgcccagg gtacctaccc 11761 agaaattgtt ccgctgatca cggtatatct tccagacgga cttattcgcc agactattcg 11821 gatcatcctc ctcatggcga taatggttaa aacgtccgtt gctgtagaag tccattcctc 11881 cgaaataata accgatccag agatcattgt agttattcac atagagactg acgatcacat 11941 ctttgctgat gctgttatca ttggaaggat catgctgaaa gcgtttgaag gtctgattga 12001 cacggtcgaa gtaaatcagt ccgccgccat tcgtaccaat ccagatattc cctttttcat 12061 cttctgcaaa gcaattgaca tcattataac tcaggctgtt attatcagca tggctatgct 12121 tatacaaggg aaatttcacc atattctcag tgaaataact aagtcctctt ttgtaggtgc 12181 cacaccagat aatgccgaaa ttatctttgt agagtgcata gacggcattc tctgcaatgg 12241 tcttcagatc gccttcttta ttcacaagaa agcgggactt aaaattgtta ttcttatcaa 12301 tcaggttaat tcctccgtga tctgtaccaa tccagatgaa atgtttatcg tcttccacaa 12361 tactattgac aatatcattg ttcaaagccc ctgttttact ggatagcatt ttcactttac 12421 catccgtccg gttcagataa gccaccccga aatcacttcc cggcatatac acccacatat 12481 cctttgctgc gtctatgaag atgcgcaggg ctaagggagg tttaccgatg tatttcctga 12541 tattggaatt gcgatagata atcttattac tggcaatgtc cagtttttcc acatctccgt 12601 tcagataaag gataaccagc agcttgtctc cttccccctg cagatcggca atagccgcat 12661 tggccctatc cggcagtaat attttagtgg tacggccggt gctgtctgtt ttgtataacc 12721 gggcatcatt ataagcgatc caccagttgc ggcctgtttg tgcgacactg gtgatgccgt 12781 attcagacaa accttttttc ttcagccagg tagtcgcatc ggagaactgt tctgtgaccg 12841 gattatagaa attgtccccg ttccttgttc tgataaaaag ctgattattc ggtgcgggat 12901 agataccggt gatgtagtcg tcgttaataa cgccaggttc tgcactatgc cggaatgttt 12961 taaacgcata accgtcataa cggtttaatc cgctcatggt accaaaccac ataaatcccc 13021 tggcatcttt ataaatggaa tttacctggt tattggataa accattgttc acatctaaag 13081 aagagaactg gtaggatggc tgtgcatagg catagcttac cagcaataac agtggaagca 13141 gcaaattttt cattaacat //