LOCUS AAXF02000047 14774 bp DNA linear BCT 04-AUG-2012 DEFINITION Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont517, whole genome shotgun sequence. ACCESSION AAXF02000047 VERSION AAXF02000047.1 DBLINK BioProject: PRJNA18191 BioSample: SAMN00627058 KEYWORDS WGS. SOURCE Bacteroides ovatus ATCC 8483 ORGANISM Bacteroides ovatus ATCC 8483 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. REFERENCE 1 (bases 1 to 14774) AUTHORS Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M., Liep,D. and Gordon,J. TITLE Draft genome sequence of Bacteroides ovatus (ATCC 8483) JOURNAL Unpublished REFERENCE 2 (bases 1 to 14774) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (15-FEB-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 3 (bases 1 to 14774) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-MAR-2007) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene: X83952) is a member of the division Bacteroidetes. In one comprehensive 16S rDNA sequence-based enumeration of the colonic microbiota of three healthy adult humans, it represents, on average, 0.034% of all 16S rDNA sequences and 0.071% of the sequences in its division (Eckburg et. al. (2005)). The sequenced strain was obtained from ATCC (ATCC 8483T). We have collected 6.9X coverage in plasmid end reads and 454 reads. We will be performing one round of automated sequence improvement (pre-finishing). Sequencing/Assembly: The genomic DNA was purified from liquid culture derived from a single bacterial colony. A hybrid sequencing strategy that utilized reads from both 454 GS-20 and ABI 3730xl sequencers was devised and implemented to generate the draft genome sequences. 454 reads were assembled using Newbler (454 Life Sciences) into 454 de novo contigs. These de novo contigs were converted in silico to 800 base paired reads ('superreads') with 400 base overlaps with neighboring superreads. Finally, PCAP (Huang, et al, Genome Research, 13:2164, (2003)) was used to assemble the super-reads and the conventional 3730xl capillary reads. This sequenced strain is part of a comprehensive, sequence-based survey of members of the normal human gut microbiota. A joint effort of the WU-GSC and the Center for Genome Sciences at Washington University School of Medicine, the purpose of this survey is to provide the general scientific community with a broad view of the gene content of 100 representatives of the major divisions represented in the intestine's microbial community. This information should provide a frame of reference for analyzing metagenomic studies of the human gut microbiome. Further details of this effort are described in a white paper entitled 'Extending Our View of Self: the Human Gut Microbiome Initiative (HGMI)' (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS eq.pdf). These studies are supported by National Human Genome Research Institute. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were blasted against NCBI'snon-redundant (NR) database and predictions generated based on proteinalignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the contig level and may notnecessarily reflect any known order or orientation betweencontigs. For answers to your questions regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. Annotation was added to the contigs in August 2007, and the CDS comments were updated in January 2008. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Product names were updated in August 2012. FEATURES Location/Qualifiers source 1..14774 /organism="Bacteroides ovatus ATCC 8483" /mol_type="genomic DNA" /strain="ATCC 8483" /type_material="type strain of Bacteroides ovatus" /db_xref="ATCC:8483" /db_xref="taxon:411476" gene 1..2871 /locus_tag="BACOVA_02051" CDS 1..2871 /locus_tag="BACOVA_02051" /inference="protein motif:HMMPfam:IPR000531" /inference="protein motif:HMMPfam:IPR012910" /inference="protein motif:superfamily:IPR008969" /inference="similar to AA sequence:INSD:AAO78058.1" /note="COG: NOG06412 non supervised orthologous group; Psort location: OuterMembrane, score:9.49" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q45780|1.B.14.6.1" /protein_id="EDO12162.1" /db_xref="InterPro:IPR000531" /db_xref="InterPro:IPR008969" /db_xref="InterPro:IPR012910" /translation="MSNDAIAQKAIRGTVLDEANEPIIGASVLVKGTTNGSITGVDGTF NVKANPSDVLVISYVGYATVEQQVGNQTQLVIHLREDSKVLNDVVVIGYGSTTKKELTG SVTSMKKDDLNPGTFTNAMGMLQGKVAGLQIINPNGADPTAKYEVLLRGTNTLSAGQGP LIIIDGVAGADIRNINFQEVESIDVLKDGSAAAIYGTRGTNGVIIVTTKRARSGKTEVS YDGQLTVGVVSRRAKPLSASEYKSVIKEYRPELESYIFDSDTDWFKEITQTPFSHKHNL SISGGSEKFSHHTSFNYEKSDGLQKHNSSEKLMARTNIRQSLLDKWVDLDYNLNIIHRK YSPSSTSAFMQAFTHNPTEPVYDDSDPDAGGYSRIKAMEYYNPVAIINERNMESKNDNY GANIRATLNILPIKGLKWENFVSYDKEQYETREYYTHYYPSLIGTNGQAYIENYQENDT QYESTLNYSNIFGKHSIQALLGYTYQYTYSTSASMTNSGFDFDDNQTHNIGTGTNLTEG KASMSSNKEDNTYIGFFGRFMYNYDDKYLLSASLRRDGSSRFGDNNKWGWFPAVSVGWR INKEKFLSNVKWIDDLKLRAGYGVTGNQDFSNYKSLMMMTTAGKFYYNGQWINTYQPAS NANPDLKWEKKAEFNVGVDMTMFDNHLSFTFDYYKRTTSNLLYDYIVPTPPYVYNTLFT NVGKVTNEGVELTISGTPFNTRDFTWNTSLTVSHNKNKLVKFTNDEFTNGTYKVGWSTS AACYTQRLIEGQSLGTFYGPIWLGTDTDGKDVLLGQNADGSVPEEQWEKIGCAYPDATL SWSNTFRYKKFDLSFSLRASIGGEILNNYAMEYENLSSIGLRNISSNWLSQTNFTSTTY KYSSKYIEDASYLKLDNVTFGYTWDFTSKMIKRLRLSLTAQNVFCITGYSGVDPEVALS GLEPGMESLSYYPRTTEFTFGVNIVF" gene 2887..4416 /locus_tag="BACOVA_02052" CDS 2887..4416 /locus_tag="BACOVA_02052" /inference="protein motif:HMMPfam:IPR012944" /inference="similar to AA sequence:REFSEQ:ZP_01719514.1" /note="COG: NOG26547 non supervised orthologous group; Psort location: OuterMembrane, score:9.52" /codon_start=1 /transl_table=11 /product="SusD family protein" /protein_id="EDO12163.1" /db_xref="InterPro:IPR012944" /translation="MKKTNIILSFVICILLYGCTDLSETVYTGVAMNDFFKNEKELVAN AGRAYTKLQGYNSEQSLWTLLLQASDECAVPACGGSWYSNGRYEEIQTNKIPPANKLLT RGWNWIFNGIAACNEIIYETELSPIQFEGKEKIIAEMKILRAFYYYQAISCWGNVPFTT DYTETGYPEQKSREYIFNYLEKEINDNIEFLDREPSDTNYGRATQAAAYCILAKMYLNA EAWFGTPMYDKAEKACKDIMDIGAYSIEDSYSTNFDIKNEDSKENIFVILYDRVYTSGD SNSFYLHTLTLEAASQATFNIPAAPWSGFLCQPDFFQTYAEQDLRRSQSWLYGPQVDLS GKDLGFEYTPVFPEEKYYNSNGGRGTYDGARCWKWHYQTDGSLKEYTVSMDNDFAIFRY ADVVLMYVEALVRQNRTSEAIQLADFKKIRTRAGLDAYTTSQLTVDELYAERGRELAWE GWRHEDMIRFGKYLKKYWAHPDQSSETFRNVFPIPTDILNANPKLSQNKDY" gene 4476..6440 /locus_tag="BACOVA_02053" CDS 4476..6440 /locus_tag="BACOVA_02053" /inference="protein motif:HMMPfam:IPR008009" /inference="similar to AA sequence:REFSEQ:ZP_01122507.1" /note="COG: NOG08360 non supervised orthologous group" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="EDO12164.1" /db_xref="InterPro:IPR008009" /translation="MKKPIIFLLVLITNILFISAQKISKTELKDKIAGAWIGQMVGNIY GLPFENKFVDEPAPESRFPFGYTKNIDKLQKYNGAFSDDDTDVEYIYLLLMEKYGVEPT YANMREGWMYHIRDRVWLANRAALGLMHLGFTPPFTGDENLNPHWYQIDPQLINEIWAY TAPGMISYAAGKSDWAARITSDSWAVSPTVLYGAMYADAFFCKDIRKLITRALKELPAD DRYAIAVKEMIALYDKYPKDWVKARQIMAKKYYIDEPAMTKTIWNANLNGLCGILAMLY GEGDFQRTLDLSCAMGFDCDNQAATISGLLGVMYGAKSLPESLTKPIEGWEKPFNDRYI NITRFDMPDASIEDMIERTYNKAIELVCAKGGKVKGDMVYVNPKAQFIPPMEFCIGPNP DLEIGQPTDYSFACRTNADFKWELVKGKLPVGVTFQNGKLAGTPTEAGKYPITLQLSAN NKNSITKDFELLVKTKNIAPKADTIYANIRKLNEEVLDSCWITFGKPMYATNVEVINDG VKDGVGSVFYSLAAKSNLPKIDYFGYGWKEEHNINMLVLDMGCLEEFGGWFTSLNIQYL GNDGHWYDVGKFKSTPALPETDIVFFQPPFAQYVLEFPAVETKGIRVLMDAKVQEHWHK YTKNVSSFISITELGVYEK" gene 6453..8060 /locus_tag="BACOVA_02054" CDS 6453..8060 /locus_tag="BACOVA_02054" /inference="protein motif:HMMPfam:IPR005502" /inference="similar to AA sequence:INSD:EDJ82860.1" /note="KEGG: mst:Msp_0683 0.0010 hypothetical protein K00788; COG: NOG08360 non supervised orthologous group" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="EDO12165.1" /db_xref="InterPro:IPR005502" /translation="MRKIITICIIGLFALNVQAQPVKTLKLSDKELLDKIKGGWAGQTI GVVFGAPTEFKFTGTYIQDYQPIPWAEGYVKYWWEKKPGLFDDIYNDCTFVEAFDELGL DCSQEELAKRFAFADYHLAHANQAGRYNIRQGIMPPASGHWLNNPHADDLDFQIEADFI GLMAPAMLPEALDIASRVGHIMNSGDGFYGGAFVAALYSSAFYEKSPEAILKTAISAIP EKSTFHQCIQDVINFHSLHPDNWKDCWYFLQEKWNCDVGCPKGVFLNFNIDAKLNSAFV ALAMLYGKGDFTNSIDIATRCGQDSDCNPSTVGGVLGVMYGYDKIPSFWLNPLKEVEDF TFEGTNMSLAKAYQMSFDQAKQLIVKTGGKVSGGEIEIPIKKADVLPLEQNFENTYPLY RERKDCFLTDTFEFDFNGNGFVIWGNICCTRSITPDYINRVSTRHIGSEVFGLAEPNDP YVAKVEIWIDGELDHVAALPMRNTDRKVEPAWKYLMKEGRHHVKMKWLNRKKDYIIRIN DIMYYSEKKEHDRFYFNK" gene 8067..9614 /locus_tag="BACOVA_02055" CDS 8067..9614 /locus_tag="BACOVA_02055" /inference="similar to AA sequence:INSD:EDJ82860.1" /note="COG: NOG08360 non supervised orthologous group" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="EDO12166.1" /translation="MKKVLINIILLFTFSISGMAQIEYGKTVEISKEVLLDKIKGGWAG QTIGCTYGGPTEFKYRGAIIHEKTPIIWYDDYCKDIFAEDPGLYDDVYMDLTFLEVMQK EGINAPAESFAKAFANADYKLWHANQAARYNILHGIMPPASGHWKNNPHADDIDFQIEA DFIGMICPGMVNTASNFSDKIGHIMNYGDGWYGGVYMGAMYALAYVNNDIYTIVTEALK TIPEQSKFHRCITDVIKYWKQYPDDWRKCWLEIENRHAFEIGCPEGVFNAFNIDATINA AYCVMGLLYGNGDFFKTMDIATRCGQDSDCNPATAAGILGVIQGYKAIPEYWKPALERC ENIKFPYTDISLSSVYDINLKLLSDVLKANGGKIKGDKYYTTIQKPKTVAWEVSFEGLY PSERRVIKYDLGTEKTFEFNGKGVVLMGMIRQDVKDSNDNYIAILEAYIDGKKVETIEM PYDYIKRKYDIFYNYDLEEGPHKLVIKWINPNEKYAVQCKDMVVYSSTPAEPINPYK" gene 9627..11225 /locus_tag="BACOVA_02056" CDS 9627..11225 /locus_tag="BACOVA_02056" /inference="protein motif:HMMPfam:IPR000801" /inference="similar to AA sequence:REFSEQ:ZP_01104988.1" /note="KEGG: pgi:PG0425 4.7e-23 esterase, putative K03929; COG: COG0627 Predicted esterase; Psort location: OuterMembrane, score:9.52" /codon_start=1 /transl_table=11 /product="CE1" /protein_id="EDO12167.1" /db_xref="InterPro:IPR000801" /translation="MNRKLLLLLALLLFSYGLSSCSSDDNNPSEGEQTDTPELFTKRYN PDQSFYSKILGQEIKYSVLLPQEYLSESTGKYGVVFLLHGWGGNQSSWGPSGLNIQSIA DAQTSNGSIRPLIYIMPEGFNTYFCNRYDGKFNYMDMFINELVPLIDKRFRTTASKTER AVAGFSMGGFGALSIASQHPETFSVSIGLSPSLNTDEQYISLSQDGWNLQWGNNFGGSG QTGTGRLTSYYKSQCPLHFFKDKPSSTFQTVRYYIDCGDDEERLYAGNGELHSLLRDKN IKHEYRVRNGAHTDSYWRESMKEALPFIERSFKGENYPQETLKKFTEELHATNKNIKVG NSNIELWLPDDYNSELTYKVLYYSKGEGNVDLTTKKVAVALDSLMQIKRMIIAGFNVKE MILNETNFSAITDAVEKTVHTESNADFRLGLTYGSEADYLYNQSTGNAPAINFFFAEDA DIINLSAENRAKIYYLDITDEGSNYNSIFTLFNGLRGAEAPVQYRVRNGLDSEQSAQTG IYSMSYYIGEQLIKK" gene 11227..12747 /locus_tag="BACOVA_02057" CDS 11227..12747 /locus_tag="BACOVA_02057" /inference="protein motif:HMMPfam:IPR000801" /inference="similar to AA sequence:REFSEQ:ZP_01104988.1" /note="KEGG: pgi:PG0425 4.9e-28 esterase, putative K03929; COG: COG0627 Predicted esterase" /codon_start=1 /transl_table=11 /product="CE1" /protein_id="EDO12168.1" /db_xref="InterPro:IPR000801" /translation="MKKILLYIILFLSGIDGAWALPIEKEGMSIYSPSLKQEVSYSIIL PEGYEHSETEYPVLYMFHGIGGDYTSWLEYGNVARVMDKMIKEGKIQPFIMVIPDGYLS YYSDTYDGSSLYETFFIKELVPYIDNNYRTRKDINGRSIIGFSMGGFGALSVSLRNRHL FGSVVALSPSIRTEKQYIEEGPQKGWDNQWGRIFGGVGKNGKQRLTSYYKQHSPYHILS TLRNSDLKGFGIMLDIGDKEGTLCESNEELHRLLLERQIPHEWEVRSGGHDFTCWNTAL PKAFRFINEYFNGKRSGNSESSLPNETPFIQTANATVYYPEQAQGSTRKYPIIYVQGEI NEQQQKVLVSQFHQMVDENKTWPAVLCFVKANTDLSETISDIEKQLSGIRGSQRMRALI TLGDNIKEGIEAIQGENLFTGIVCVNAIGNENDAQNLIKAVNSYKRYPRCWIEILPESK EYGFSSNIHILLKESDLEHEFRSRKCKEANVFTYWEDWILYLNNRIHV" gene 12817..13773 /locus_tag="BACOVA_02058" CDS 12817..13773 /locus_tag="BACOVA_02058" /inference="protein motif:HMMPfam:IPR010651" /inference="similar to AA sequence:REFSEQ:NP_811721.1" /note="COG: NOG04879 non supervised orthologous group; Psort location: CytoplasmicMembrane, score:10.00" /codon_start=1 /transl_table=11 /product="fatty acid elongase 3-ketoacyl-CoA synthase 1" /protein_id="EDO12169.1" /db_xref="InterPro:IPR010651" /translation="MICWGSWGNTQKLVSKNWRYELFYWDYVIGMVLFTILLGFTMGSH GDTGRSFLEDLGQASGDSIGWVILGGVIFNASNILLSASISLAGMSVAFPLGVGIALVL GVIVNYLGIPTGNPLLLFGGVALIVIAIICNGVASGKMQKGEESRKNNKKGIIIALIAG VLMSLFYRFVVKGMDVENFNSPAIGMMTPYSAIFVFSIGVLLSNFVFNTLVMKCPFVGE KVSYSEYFKGNTKTHICGMLGGAIWGLGSAFSYIASGEAGPAVSYALGQGAPLIAAIWG VFIWKEFKGSTKSVNQLLALMFIFFLVGLGMIVVAGN" gene 13797..14774 /locus_tag="BACOVA_02059" CDS 13797..14774 /locus_tag="BACOVA_02059" /inference="protein motif:Gene3D:IPR008957" /inference="protein motif:HMMPfam:IPR003961" /inference="protein motif:HMMSmart:IPR003961" /inference="protein motif:superfamily:IPR008957" /inference="protein motif:superfamily:IPR011050" /note="KEGG bcl:ABC0646 0.0016 chitinase K01183" /codon_start=1 /transl_table=11 /product="fibronectin type III domain protein" /protein_id="EDO12170.1" /db_xref="InterPro:IPR003961" /db_xref="InterPro:IPR008957" /db_xref="InterPro:IPR011050" /translation="MKKYFYLLAMLAVVLGTTACSDDDNHPSTNILDKPEVTVPDVKES SAVITWKAIGNATAYIYSLNNGSEQSTDQNTIQLTGLEPEKSYTFKVKAQKTGSIYFED SDYAEITFTTTSEVTVYRIATFADDWDKWYYEYNDNGTVKRVYRLYEGELDREWLFAYD GNNITVTGKNAYNMTLNDQGYVATFVDGSNTYEYTYDENGYMTKVEKNGNIASNITIEN GNIMQWTRFSDGVEQFKVQTYSAVPNVSGAHCIYAESSGASRWLVETGLFGKASANCHT SSGWQHSSSTATYTFEYDENSCIKEESKNYDGYIENFYYTYFSE" ORIGIN 1 atgagcaatg atgctattgc ccagaaggca ataagaggta cagtcctcga tgaagcaaat 61 gaacccatta tcggtgccag tgtccttgtt aaaggtacaa caaatggttc tattacagga 121 gttgatggaa ccttcaatgt aaaagcgaat ccttctgatg tattggttat atcttatgta 181 ggttatgcaa ctgttgaaca acaagtagga aatcagacac agcttgtaat acacctgcgc 241 gaagactcga aagtgcttaa tgacgttgta gtaatcggct acggctctac cacaaagaaa 301 gagcttacag gatcggttac cagcatgaag aaagatgacc tcaatcccgg aacattcacc 361 aatgctatgg gaatgttgca aggaaaagtg gcaggattac aaattatcaa cccgaacggt 421 gctgatccga cagccaaata tgaagtatta cttcgcggaa caaacacact ttccgcaggc 481 caaggtccat taattattat tgatggggtg gcaggtgcag atatcagaaa tatcaatttt 541 caggaagtgg aatcaattga cgtattaaaa gatggttcgg cagctgctat ttatggaaca 601 agaggtacga atggagtaat catcgtcaca actaaacgtg caagaagtgg caaaacggaa 661 gtttcttatg atgggcaact caccgtggga gtcgtttcac gcagagcaaa acctttatcg 721 gcaagcgaat acaaatctgt tatcaaggaa tatcgcccag aattggaaag ttatattttt 781 gacagtgata ccgattggtt taaagaaatt acacaaactc cgttcagcca taagcacaac 841 ttgtctatat ccggaggttc cgagaagttc tcacaccaca cttctttcaa ttatgaaaag 901 agcgatggct tgcaaaagca taatagctcc gaaaagctaa tggcacgtac caatattcgc 961 cagtcattat tagataaatg ggtcgatttg gattataacc tgaatatcat tcatcgcaaa 1021 tacagccctt cctctacaag tgcttttatg caggcattta cccataatcc aacagagcct 1081 gtttatgacg acagcgatcc ggatgccgga ggatatagcc gtatcaaagc aatggaatat 1141 tacaatccgg tagcgatcat taacgaaagg aacatggaaa gtaagaatga taattatggt 1201 gcaaatattc gtgctactct aaacatattg cctataaaag gattaaaatg ggagaacttt 1261 gtttcatacg acaaggaaca atatgagaca cgtgaatact acacacatta ttatccaagt 1321 ttaataggaa caaacgggca agcctatatt gaaaactatc aggaaaacga tacacaatat 1381 gagtctactt taaattactc gaatattttc ggaaaacatt ccattcaggc attactcgga 1441 tatacatacc agtatactta cagcacatct gcatccatga caaattccgg tttcgacttc 1501 gatgacaacc agactcataa catcggaaca ggaacgaatc taactgaagg caaagccagc 1561 atgtcgtcca ataaagaaga taatacatat atcggattct tcggacgttt tatgtacaac 1621 tacgatgata aatatctttt gtctgcttca ctacgtcgtg atggctccag ccgtttcggt 1681 gacaataata agtggggatg gttcccggct gtcagcgtag gttggcgtat aaataaagag 1741 aaattcctct cgaatgtcaa atggatcgat gatttaaaat tgagagccgg atacggagtt 1801 accggtaacc aggatttctc aaactacaaa tctttgatga tgatgactac agccggtaaa 1861 ttctattaca atggccaatg gatcaatact tatcaacctg caagtaatgc aaatccggat 1921 ctaaaatggg agaaaaaagc agagttcaac gttggtgtcg atatgacaat gtttgacaac 1981 cacctctcgt tcacttttga ttattacaaa cggactacat caaacttgct atatgactac 2041 atcgtgccaa cacctcctta tgtatataat accttgttta cgaatgttgg aaaagttact 2101 aatgaaggag ttgaattaac aatatccggt acacctttta acactaggga ctttacatgg 2161 aatacctcac ttaccgtttc gcacaataaa aacaaattgg tgaaattcac caatgatgaa 2221 ttcactaacg gaacatacaa agtgggatgg tctacaagtg ctgcctgcta cactcaacgt 2281 ctgatagaag gacagtcatt gggtactttc tatggtccta tatggttagg aactgataca 2341 gatggaaaag atgtattgct aggacaaaat gcagacggtt ccgtaccgga agaacaatgg 2401 gaaaaaattg gctgtgctta tccggatgcc actcttagct ggagtaatac cttcagatat 2461 aagaaattcg acttgagttt ctcactgcgt gcatctatcg gcggagaaat tctgaacaac 2521 tatgcgatgg aatatgaaaa tctaagcagc atcggactta gaaatatctc atcaaactgg 2581 ttatctcaaa cgaatttcac aagtacgacc tataaatatt cctccaaata tatagaggat 2641 gcttcttatt taaaactgga taatgtaacc tttggatata cttgggattt cacatccaaa 2701 atgataaaaa gactaagatt gtctcttact gcacagaatg tattctgtat caccggttat 2761 tctggtgtcg acccagaagt tgctctttcc ggactggaac ccggtatgga aagtttgagc 2821 tattatccgc gtacaacaga atttactttt ggagttaata tagtattcta aaaaaataag 2881 attaccatga aaaagacaaa tataatatta agtttcgtta tctgtatatt attatacgga 2941 tgtaccgatc ttagcgaaac tgtatacaca ggagtagcga tgaatgactt ctttaaaaat 3001 gagaaagaac tggtagccaa tgcaggtaga gcgtatacga aattacaagg atacaattcc 3061 gaacaaagtt tatggacatt gcttttacaa gcttctgatg aatgtgccgt tcccgcttgt 3121 ggtggttcct ggtacagcaa tggacgttat gaagaaatcc agacgaataa aattccacct 3181 gcaaataaat tattgacaag gggatggaac tggatattta atggtattgc tgcatgtaat 3241 gaaattattt acgaaacgga attatcacct atccagtttg aaggaaaaga gaagattata 3301 gctgaaatga aaatattgcg tgctttctac tattatcaag ccatttcttg ttggggaaac 3361 gtacctttca caactgacta cacggaaacc ggatacccgg aacaaaaaag tcgtgaatat 3421 attttcaact atctggaaaa agaaattaat gacaatatcg agttcctaga cagggagcct 3481 tcagatacta attatggacg ggccacacaa gctgccgctt attgcattct tgcgaaaatg 3541 tatctgaatg cagaagcatg gttcggtact ccaatgtatg acaaagcaga gaaagcctgt 3601 aaagatatta tggatatagg tgcttacagc attgaagact cttattctac taattttgat 3661 ataaaaaatg aagattctaa ggagaatatc tttgtaatcc tgtatgacag agtatatact 3721 agcggtgaca gtaactcatt ttatttgcat acgctgacat tggaagctgc atcacaagca 3781 acatttaata ttccggcagc cccatggtcg ggttttctct gccaaccgga ctttttccaa 3841 acatacgcag aacaagatct tcgcagaagt cagagctggt tatacggtcc tcaagtagac 3901 ctaagcggaa aggatcttgg tttcgaatat actcctgtct tccccgaaga aaaatattac 3961 aatagcaacg gaggacgtgg cacatacgac ggtgcacgct gctggaaatg gcattaccag 4021 actgatggtt ctttaaaaga atatacagtt agtatggaca acgactttgc catcttccgt 4081 tatgccgatg ttgtattaat gtatgtagag gctttggtac gacaaaacag aacaagtgag 4141 gccatccaac tggcagactt caagaagatt cgtacaagag cagggcttga tgcatatact 4201 acaagccaat tgacggttga tgaactttat gcagaacgag gcagagaact ggcatgggaa 4261 ggatggcgcc atgaagatat gatacgtttc ggaaaatatc tgaaaaaata ttgggcacac 4321 cctgaccaaa gttcagaaac ttttagaaat gtatttccaa ttcctacgga tattttaaat 4381 gcaaatccaa aattatctca aaacaaagat tattaaccac aattggaagg ttcacataga 4441 aaagtgtacc ttccaacaaa tcaaccctga taagtatgaa aaaaccaatc atctttttac 4501 ttgttttaat taccaacatc ttatttatct ctgcacagaa aatttcaaag acagagttaa 4561 aagacaaaat agctggagct tggatcgggc aaatggtagg aaatatatat ggacttcctt 4621 ttgagaacaa gtttgtagat gaacctgccc cggaatccag atttccattc ggttacacta 4681 aaaacataga taaattgcag aagtataacg gtgcattctc tgatgacgat actgatgttg 4741 agtatatcta cttactatta atggagaaat atggtgtaga acctacttat gccaatatgc 4801 gcgaaggatg gatgtatcat atccgtgacc gtgtatggct agccaatagg gctgcactcg 4861 gattaatgca tcttggattc acccctccgt tcacaggtga cgaaaatctg aatccgcact 4921 ggtatcagat cgaccctcaa ttgattaacg agatatgggc gtatacagca ccgggaatga 4981 tttcatatgc tgcaggaaaa agtgattggg cggcccgtat cacaagcgat tcgtgggcgg 5041 tatctcccac tgtcctatat ggtgcaatgt acgctgacgc ttttttctgc aaggatatcc 5101 gcaaactgat tacacgagcc ttgaaagaac tacctgctga tgacagatat gcgatagctg 5161 taaaagagat gattgcttta tatgacaaat atcctaaaga ttgggtaaaa gcacgtcaaa 5221 tcatggctaa aaaatattac atagatgaac cagccatgac aaaaacaatc tggaatgcta 5281 atctgaacgg attatgcggg attttggcta tgttgtatgg cgaaggagat ttccaacgga 5341 cattggattt aagctgtgca atgggatttg actgcgacaa tcaggcagcc actatctccg 5401 gacttctagg agtaatgtat ggtgcaaagt ctcttcccga aagtttgact aaacctatcg 5461 aaggatggga aaaacccttc aacgaccggt atatcaatat caccaggttt gatatgcccg 5521 atgcttccat tgaagacatg attgaacgta cttacaacaa agcaatcgaa ctggtttgtg 5581 ccaaaggagg aaaagtaaag ggtgatatgg tatatgttaa ccccaaagca caattcatcc 5641 cccctatgga attctgtatc ggtccgaatc cggacttgga aatcggacag cctaccgatt 5701 actcatttgc ctgcagaaca aacgctgatt tcaaatggga actggtaaaa ggtaaacttc 5761 cggtaggagt tacattccaa aacggtaaac tggcaggaac tcctacagaa gccggcaaat 5821 atccaattac ccttcaatta agcgcaaata acaagaactc aattactaaa gactttgagt 5881 tattggttaa aaccaagaat atagccccca aggctgacac tatttacgca aatattcgca 5941 aactaaacga agaagtactc gattcttgct ggattacctt tggaaaaccg atgtacgcaa 6001 caaatgtaga agtaattaat gacggagtca aagatggcgt aggatctgta ttttattcat 6061 tagcggccaa aagtaatctg cctaaaatag actatttcgg atacggttgg aaagaagagc 6121 ataacatcaa tatgcttgtt ttagatatgg gctgtctgga agagttcgga ggatggttta 6181 cttctctaaa cattcaatat cttggaaatg acggacattg gtatgacgta ggtaaattca 6241 aatcaactcc ggcacttccc gaaaccgata ttgtattttt ccaacctccg tttgcacaat 6301 atgttttgga attcccggca gtagaaacca agggaatacg cgtactaatg gatgcaaaag 6361 tgcaggaaca ctggcataaa tacacaaaga atgtatcatc attcatctct atcaccgagt 6421 tgggagttta tgagaaataa aggaggagaa gaatgaggaa aataattacg atatgtatta 6481 tagggctttt tgctctcaac gtacaagccc agcctgtaaa aactttaaag ttatctgata 6541 aagaactttt agataagatt aaaggaggat gggccggaca aaccattggt gttgttttcg 6601 gagcacctac agagtttaag ttcacgggaa cttatattca agattaccag ccaatcccct 6661 gggcggaagg ttacgtaaaa tattggtggg aaaagaagcc cggattgttc gatgatattt 6721 ataacgattg cacgtttgtt gaggcatttg atgaacttgg cctggattgt tcacaagaag 6781 aacttgccaa aagatttgca ttcgctgatt atcatttggc ccatgctaac caagcaggca 6841 gatataatat ccgccaaggt atcatgccac cggcttccgg tcattggttg aataacccac 6901 atgcagacga tctggatttc cagatcgaag cagacttcat tggtctgatg gctccggcaa 6961 tgctgccgga agcattggat atcgcctcca gagtaggcca cattatgaat agcggtgacg 7021 gtttttatgg cggtgctttc gtagcagctc tttatagctc cgctttctat gaaaaaagtc 7081 ctgaagcgat tctaaaaacc gcaatctctg ccatacctga aaagagtaca ttccatcaat 7141 gtattcagga tgtaatcaat ttccattctt tgcacccgga caactggaaa gattgctggt 7201 acttcctgca agaaaaatgg aactgcgatg taggatgtcc caaaggcgta tttctaaact 7261 ttaatattga cgccaagctc aattccgctt tcgtagcatt agctatgctc tacggtaaag 7321 gagacttcac caactctatc gatattgcaa cacgctgcgg acaagattct gactgtaatc 7381 cgtctacagt cggcggtgta ctcggcgtca tgtatgggta tgataaaatc ccttcattct 7441 ggttaaaccc tttgaaagaa gtagaagact ttacatttga aggaacgaat atgtcgttgg 7501 caaaagcata ccaaatgagt ttcgatcaag ctaaacaact catagtcaaa acaggtggaa 7561 aagtatctgg tggggagata gaaattccaa tcaaaaaggc ggatgtactt cctttagaac 7621 aaaatttcga aaacacctat ccactttata gagaacgcaa agactgcttc ctgacagata 7681 catttgaatt tgatttcaat ggtaacggat ttgttatctg gggcaacatt tgctgcacaa 7741 gaagtattac tcccgattat atcaatcgtg tatctacacg ccacatcggt tccgaggtgt 7801 tcggattggc agagcccaat gacccttacg ttgctaaagt ggaaatatgg attgacggag 7861 aactggacca tgtagcagct ttacccatga gaaacacaga ccgcaaagta gaaccagcct 7921 ggaaatacct catgaaagaa ggaagacacc acgttaaaat gaaatggttg aaccgcaaaa 7981 aagactacat tattcgtatc aacgacataa tgtattattc agagaaaaaa gaacatgata 8041 gattttactt taataaataa gttaagatga aaaaagtcct gattaacatc attctactat 8101 ttaccttttc catatcggga atggcacaaa tagaatatgg aaaaacagtt gaaataagta 8161 aagaggtact gctcgacaaa attaaaggag gctgggccgg acaaaccata ggatgcacct 8221 atggcggtcc tacagaattt aaatacagag gagcgatcat tcatgagaaa actccaataa 8281 tctggtatga tgactattgc aaggacatct ttgcagaaga tccgggattg tatgatgatg 8341 tatatatgga tcttaccttt ctggaagtaa tgcaaaaaga agggataaat gcccctgccg 8401 aatcatttgc caaagccttt gcaaacgcag attacaaatt atggcatgcc aaccaggcag 8461 ccagatataa catattgcac ggtattatgc caccagcatc gggacattgg aaaaataatc 8521 cgcacgctga cgatattgat ttccagattg aagcagactt cattggaatg atatgtccgg 8581 ggatggtaaa taccgcctcc aatttttcgg acaagatagg gcatatcatg aactacggcg 8641 acggatggta tggaggtgta tacatgggag caatgtatgc attggcttac gtaaataacg 8701 acatttatac aattgtcacg gaagctctta aaacaatacc cgaacaaagt aaatttcacc 8761 gttgcatcac agacgttatt aaatattgga aacaatatcc cgatgactgg cgtaaatgct 8821 ggctggaaat agaaaaccgg catgcttttg agattggctg cccggaaggg gtattcaatg 8881 catttaacat tgacgccact atcaatgcgg cttactgtgt gatgggactt ttatatggca 8941 acggcgactt tttcaaaaca atggatatag ccactcgttg cggacaagat tccgactgta 9001 acccggcaac tgccgcagga attcttggag tcatacaagg ctacaaggct atccccgaat 9061 actggaaacc ggcactcgaa agatgtgaaa atattaagtt cccttacaca gatatctctt 9121 taagttctgt atacgacatt aatctcaaac tattgagtga tgtattaaaa gcaaacggag 9181 gaaaaataaa aggagataaa tactatacca cgattcagaa gccaaaaacc gtagcatggg 9241 aagtttcttt tgaaggctta tatccttctg aaagaagagt tatcaaatat gatctcggta 9301 cagaaaaaac atttgagttc aatggcaagg gagttgtttt aatggggatg atcaggcaag 9361 atgttaaaga tagtaatgac aattacatcg ccatcctgga ggcctacatt gacggcaaga 9421 aagtagaaac catagaaatg ccttatgact acataaaaag aaagtatgac attttctata 9481 attatgattt agaggaagga cctcacaaac tggttattaa atggattaat ccgaatgaaa 9541 aatatgccgt tcaatgtaaa gacatggtgg tttattcatc cacaccggca gagccaatca 9601 atccttataa ataaaagaga gaagccatga atagaaaact actcctatta ttagccttat 9661 tattattttc ctacggattg tcatcatgtt cgtctgatga taacaatccg tccgaaggcg 9721 aacagaccga tacaccggaa ctgtttacca aacgctataa tcccgatcaa agtttttaca 9781 gcaaaatatt aggacaagaa atcaaatatt ccgtattact gcctcaagag tatctatcgg 9841 aaagtacggg taaatatgga gtagttttcc ttctacatgg ctggggagga aaccaaagtt 9901 catggggacc ttcaggtctg aatattcaaa gcatagctga tgcgcaaacc agcaatggta 9961 gtattcgccc attgatctac atcatgccgg aaggtttcaa cacttatttt tgcaaccgct 10021 atgacggaaa gttcaactat atggatatgt tcattaatga actcgtaccc ctgattgata 10081 agcggttccg aacaacagcc agtaagacgg aaagagctgt tgccggattc tctatgggag 10141 gtttcggggc attaagtata gcatcgcaac atcctgaaac attctcggta tcaataggat 10201 taagtccctc tctaaataca gatgaacaat acatctcttt gtcacaagac ggttggaatc 10261 ttcaatgggg aaacaacttc ggaggaagcg gccagacagg aacaggcagg ctgacaagct 10321 attataaaag ccaatgtccg ctacactttt tcaaggataa accaagttcc acatttcaaa 10381 cagtacggta ttacatagac tgcggtgatg atgaagaaag actatatgca ggaaacggtg 10441 aattacattc tctattacgg gacaaaaata tcaagcacga atatcgtgta cgaaacggtg 10501 cccatactga cagttattgg cgtgaatcca tgaaagaggc actgcctttt atcgaacgtt 10561 cattcaaagg agaaaactat ccgcaggaaa ctctgaaaaa attcacagag gaattacatg 10621 ctactaataa aaacataaaa gtaggtaatt ccaatatcga actatggtta ccggatgact 10681 acaattccga actaacttac aaagttcttt attacagcaa aggagaaggt aatgttgatc 10741 ttacaacaaa gaaggtagca gtggcattag attctcttat gcaaatcaaa cgtatgataa 10801 ttgcaggttt taatgtgaaa gaaatgattc taaatgaaac aaatttttca gctattaccg 10861 atgctgtgga aaagacggtt catacggaaa gcaatgcaga tttcagatta ggattaacat 10921 acggttcaga agctgattac ttatacaatc agtcaacagg caatgcccct gctatcaact 10981 ttttctttgc agaagacgca gatattatca acctttctgc cgagaatcgt gccaaaatct 11041 attatttgga cataacagac gaaggcagca actacaatag catctttact ctgttcaatg 11101 gattgcgagg tgcagaagct ccggtgcaat atcgtgtcag aaacgggctg gatagtgaac 11161 agtccgccca aacaggtatt tattcaatga gttattatat tggtgaacag ttaataaaga 11221 aataatatga agaagatact gttatatatt atcctatttc tatcaggtat agatggagct 11281 tgggcacttc ccatagaaaa agaagggatg agtatttaca gtccgtccct aaagcaagaa 11341 gtctcctact ccattatctt accggaaggt tatgaacatt cagaaacaga atatccagtc 11401 ttatacatgt ttcatggaat aggaggtgat tataccagtt ggctcgaata cggcaacgtt 11461 gcccgtgtaa tggataaaat gataaaagaa ggaaagattc aacctttcat tatggttatt 11521 cctgacggtt acctgtctta ttacagtgac acttatgatg gcagttccct ttatgaaact 11581 ttctttatca aagaactggt tccatatata gataacaact atcgtactcg caaggatatt 11641 aatggacgct cgatcattgg tttctctatg ggaggattcg gagcattatc cgttagtttg 11701 cgcaaccgcc acttattcgg ctctgtcgtt gcgttatcgc catccatccg tacagaaaaa 11761 caatacatag aagaagggcc acaaaaagga tgggacaatc aatggggacg gattttcgga 11821 ggagtgggaa agaatggtaa gcagcgccta accagttact ataaacaaca ttcaccctac 11881 cacattcttt ccaccctccg caattccgat ttaaaaggat ttggcatcat gctggatatt 11941 ggagataaag aaggtactct ttgcgaaagt aatgaagaac tgcaccgatt attactggaa 12001 agacaaatac ctcatgaatg ggaagtacgt tcaggaggac atgatttcac ctgctggaat 12061 actgcactgc ccaaagcatt cagattcata aacgaatatt tcaatggaaa gcggtcagga 12121 aatagtgaga gtagtcttcc gaatgagact ccgtttatcc aaacagccaa tgcaacggtc 12181 tattatccgg aacaggctca aggaagcaca agaaaatatc ctattatcta tgtacaggga 12241 gaaattaacg aacaacagca aaaggttctc gtcagtcagt ttcatcaaat ggtagatgag 12301 aataaaacat ggcctgctgt tctttgtttc gtaaaagcta acacagacct gtcagaaaca 12361 atttctgata tagaaaagca actctccggc atcagaggtt cccaacgtat gcgggcttta 12421 atcactttgg gagataatat caaagaaggt atagaagcca ttcagggaga aaatttgttt 12481 accggcatag tctgtgtaaa tgccatcggc aacgaaaatg atgctcaaaa tcttataaaa 12541 gcagtcaact cttacaaaag atatcccaga tgctggatag agatactccc cgaatcaaaa 12601 gaatatggtt ttagtagtaa tatacatatc cttttgaaag aatcggacct ggaacatgag 12661 ttccggtcaa gaaaatgtaa agaagccaat gttttcactt attgggaaga ttggatacta 12721 tatttaaata acagaattca tgtttaacaa ttaaatacta atagtatgtt cacagtaaac 12781 agttatttat tggcagttat attctgcatc gtaaccatga tttgttgggg ttcctggggt 12841 aatacccaaa aactagtttc caaaaattgg cgttatgaac ttttttattg ggattatgtg 12901 attggaatgg tattgttcac catcttacta ggcttcacaa tgggtagtca tggagatacc 12961 ggacgttcat tcctggaaga tctgggacaa gcatcaggag acagcattgg ttgggttatc 13021 ttggggggcg taatatttaa tgcttcaaac attcttcttt cagcgtctat ttccttggct 13081 ggtatgtctg ttgcttttcc tttaggagtt ggcattgcat tggtattagg ggtaatcgtc 13141 aattatctgg gaattccaac tggtaatcct ttgcttttat ttggcggtgt agctctcatt 13201 gttattgcta tcatttgcaa tggagtggct tccggaaaaa tgcaaaaggg agaagaaagc 13261 aggaaaaaca acaaaaaggg aattatcatt gcattaattg ccggtgtact gatgtctttg 13321 ttctacaggt tcgtagtgaa aggtatggac gtagaaaact tcaattctcc tgctatagga 13381 atgatgactc cttattctgc catctttgtt ttttcaatag gcgtattact aagcaatttt 13441 gttttcaaca cattagtaat gaaatgtcct tttgtcggag aaaaagtcag ctactccgaa 13501 tactttaaag gaaacacgaa aactcatata tgcggaatgc ttggtggagc gatatgggga 13561 cttggttccg cgttcagcta tatcgcatca ggagaagctg gtccggcggt atcttatgca 13621 ctaggacaag gcgcaccact tattgccgct atttggggag tatttatctg gaaagaattt 13681 aaaggttcaa cgaaatctgt taatcaacta ctggccttga tgtttatctt cttcctggtt 13741 ggtttgggaa tgatagtagt tgccggaaac taattattaa ccttaaatag aacaacatga 13801 aaaagtattt ttacttatta gccatgctgg cagtagtact aggtacgact gcttgcagtg 13861 atgacgacaa tcatcccagt acaaacattc tggataaacc ggaagttact gttcctgatg 13921 taaaagagag ttctgctgtt attacatgga aagccatagg aaacgcaaca gcttatattt 13981 atagcttgaa caacggtagt gaacaaagta ctgaccaaaa tacaattcaa ctgacaggtt 14041 tagagccgga aaaaagttat acattcaaag taaaagctca aaaaaccgga tcaatctatt 14101 ttgaagattc agactatgca gaaatcactt ttacaacaac ctctgaagta acagtttatc 14161 ggatagccac ttttgcggac gactgggata aatggtatta cgaatataat gacaatggta 14221 cggttaaacg tgtctacagg ctatatgaag gagagttgga tagagaatgg ctctttgctt 14281 atgacggaaa taatatcaca gtgacaggta agaacgcata taacatgact ttaaatgacc 14341 aaggttatgt tgcaactttt gtagatggtt ccaacactta cgaatatact tatgatgaga 14401 atggctacat gaccaaagtt gaaaagaatg gcaatattgc atctaacatc accatagaaa 14461 atggaaatat tatgcaatgg accagatttt cagatggagt agagcagttt aaggttcaga 14521 cctattctgc cgtgcctaat gtgagcggag cacattgcat ctatgcggaa agcagtggag 14581 ctagtcgttg gctggttgaa actggtctct ttggaaaagc ttcggctaat tgccatacga 14641 gcagtggttg gcaacattca tccagtacag ctacttacac attcgaatat gacgaaaata 14701 gttgtatcaa agaggaaagc aaaaactatg acggatatat agagaatttc tattatacat 14761 atttctccga atag //