LOCUS AB034969 8400 bp DNA linear BCT 15-AUG-2001 DEFINITION Thermococcus sp. B1001 cgtB, cgtA, cgtC, cgtD, cgtE genes for cyclomaltodextrinase, cyclomaltodextrin glucanotransferase, cyclomaltodextrin binding protein, cyclomaltodextrin transport membrane protein, complete cds. ACCESSION AB034969 VERSION AB034969.2 KEYWORDS cyclomaltodextrin transport membrane protein; cymF; cyclomaltodextrin binding protein; cymE; cyclomaltodextrinase; cyclomaltodextrin glucanotransferase. SOURCE Thermococcus sp. B1001 ORGANISM Thermococcus sp. B1001 Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus. REFERENCE 1 AUTHORS Hashimoto,Y., Yamamoto,T., Fujiwara,S., Takagi,M. and Imanaka,T. TITLE Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001 JOURNAL J. Bacteriol. 183 (17), 5050-5057 (2001) PUBMED 11489857 REFERENCE 2 (bases 1 to 8400) AUTHORS Takagi,M., Hashimoto,Y., Fujiwara,S., Yamamoto,T. and Imanaka,T. TITLE Direct Submission JOURNAL Submitted (15-NOV-1999) Masahiro Takagi, Japan Advanced Institute of Science and Technology, Hokuriku, School of Materials Science; 1-1 Asahidai Tatsunokuchi, Nomi-gun, Ishikawa 923-1292, Japan (E-mail:takagi@jaist.ac.jp, Tel:+81-761-51-1650, Fax:+81-761-51-1655) COMMENT On May 17, 2001 this sequence version replaced AB034969.1. Sequence updated (27-Jan-2000). FEATURES Location/Qualifiers source 1..8400 /organism="Thermococcus sp. B1001" /mol_type="genomic DNA" /strain="B1001" /db_xref="taxon:91619" regulatory 235..239 /regulatory_class="ribosome_binding_site" gene 248..2230 /gene="cgtB" CDS 248..2230 /gene="cgtB" /EC_number="3.2.1.54" /note="CDase" /codon_start=1 /transl_table=11 /product="CBM34|GH13|GH13_20" /protein_id="BAB18100.1" /translation="MYKIFGFKDNDYLGKVGITEFSIPKSGSYAYLLGNFNAFNEGSFR MREKGDRWYIKVELPEGIWYYTFSVDGNLILDFENNEKTVYRRLSYKFEKTVNVAKIFS GEKFYHYPSLVYAYSLGDSTYIRFRAMKGVAKRVFLISDQKYEMRKKAQDELFEYFEAV LPRKEGLEYYFEIHEADEIIDYGDFKVDFNEQKERFKPPAWVFERVFYQIMPDRFANGN PENDPHNCIEFKTITHHGGDLEGIIEKLDYIEELGVNALYLTPIFESMTYHGYDIVDYY HVARKFGGDEAFEKLMQKLKKRDIKLILDGVFHHTSFFHPYFQDVVKNGKNSKYKDFYR IISFPVVPEEFFEILNSKLPWDEKYRRLKSLKWNYESFYSVWLMPRLNHDSKGVREFIR NIMEYWIKKGADGWRLDVAHGVPPEVWEEIREKLPSNVYLVGEVMDDARLWIFNKFHGT MNYPLYEAILRFFVTREINAEQFLNWLELLSFYYGPAEYVMYNFLDNHDVDRMLSLLGD KRKYLCALVFLFTYKGVPSIYYGNEIGMKNIEAPFMERSRAPMEWNKKKWDKEILKTTK ELIKLRRRSKALQKGIFKPVKFKDKLLVYKRVLNNENILVAINYSKKEKHLDLPPSFEI LFQSGSFDRVNIRLKPFSSIIAKKL" regulatory 2402..2409 /regulatory_class="ribosome_binding_site" gene 2414..4633 /gene="cgtA" CDS 2414..4633 /gene="cgtA" /EC_number="2.4.1.19" /note="CGTase" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q05839|8.A.9.1.1" /protein_id="BAB18101.1" /translation="MRSRKIYMLVVLLLFLGFSEQISTVAAGVSPSYPAGDPQTWVIYQ IVIDRFYDGNTSNNDPLKSPGLYDPNKENWKLYWGGDLEGIIAKLPYLYELGVSAIWIS PVFDNIDVPINGSNGLEAGYHGYWPKDFKVIEEHFGTWEIFRRLSQEAAKYNITIIIDF VPNHSNPNDAGEYGALYDNGTFVIDYPTDANYATVHPITKSLSYIYNHNGGITNWNDRW EVRYKNLFNLADLNQLNPWVDNYLKESTVSYLEAGIGGIRIDAVKHLEPGWLKTYADYV YARKNVFMFGEWYQGFNDEMYWDMVKFANYTGIGLINIPLQQVLVDVFAYDTKTMWDLE SAVNKYTIDFMWQNKLTIFVDSHDVPRFLSLRNDLIRFHQALAFVLTAPGIPIIYYGDE QYLHNDTVNDFGQVGGDPYNRPMMKTWNTSTTAFKLIKTLASLRRYNPALAYGLIATRY VSDDIYIFERKFFDNVVLVAINRNLNSPYVVSNVYTSLPDGSYNDYLGGLLNGVGITVS SGTFSVELPAGSVSVWQYKATPTDPWVGAIDPVMGRAGNIVTVSGEGFGDVPGRVLITN GQDYWTAEVTYWSDKSVEFIVPSGITTQLNENHVEVRIERADGATSNGIAFEYLTNKQI PAIFEVRNTQGTNLETQVGEFLWLTGSVPELSYWSPETIKAVGPMLCPGWPDWFVVASV PADTYIEFKFLKAPLGGTGIWEVGSNHAYLTPSSGIGEVSVEANR" regulatory 4766..4769 /regulatory_class="ribosome_binding_site" gene 4779..6104 /gene="cgtC" CDS 4779..6104 /gene="cgtC" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|P58300|3.A.1.1.16" /protein_id="BAB18102.1" /translation="MKKALFALLLIGVLAIGVVASGCIGGGGTTSSPTQTTQTTTTSSP TQTTTTTSSPTQTTTTTTEEKKVTIVIWHAIGPNELKAFEDLIAEFEIEYPNIDIQLEQ KADLETSLKAAIPAGQGPDLFMWAHDWIGKFAEAGLLEPIDEYITPEILNKFSPMAQNA IEYGGHYYAMPYAAETVALIYNKDMVPNPPKTFDEMKAIMEKYYNEAEGTYGLATPMDP YFLSGWVHAFGGYYFDDKTKTPGLDKPETLQGFKFFFEQIFPYVAKTQDYNAQVSLFHD GKAPMMINGPWSIPDVRNAGINFGIVPLPPIDDKHRPHPYGGVRLIYVAKLNDPSKKDA IWTFLKWFTTNPDVIKTLSLQNGYIPVLTEVLNDPEIQNDPVLYGFGQAVQYAIPMPKS PEMGAVWGPVGTAITDIIAGKKTIEQALQDAQKEILDAISGS" regulatory 6137..6141 /regulatory_class="ribosome_binding_site" gene 6151..7053 /gene="cgtD" CDS 6151..7053 /gene="cgtD" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q8TZP9|3.A.1.1.16" /protein_id="BAB18103.2" /translation="MDKKTIAALALILPGMIAFLFFNIYPILYSIYIAFTNAKLGNFPI QAPTAEPLRFVGLENFRWALSDPKFRSAFVWTWVFVATSVTLKVVVGILLSVLYNNKYV KGKFFYRALLIIPWALPLLFSVMVWRFMFDPVVGPINIMLRDLGITNPPDWMTSVTWGF VALNIIEVWLAYPFMMTVITSALQSVPDTLIEAAIIDGANYWQRLTKVVIPIVSKPIAF ATILTSAASFQYFLVPFIYNAGLFEDRFLLLYGYRKAFGSSVPHYGRAAAILLIATIVL AVYMFVNMRITRLQEGVKG" regulatory 7035..7042 /regulatory_class="ribosome_binding_site" /gene="cgtD" gene 7050..8393 /gene="cgtE" CDS 7050..8393 /gene="cgtE" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q8TZQ0|3.A.1.1.16" /protein_id="BAB55639.1" /translation="MKLKVELPKRKDELLKSFVLTLLALFVLAILLFPVYYMAMLSVKP SGTLATTEIDLIPDKVTFSNYRDLLFGHTEGLIKTTNFEINAKKGTIKDSLNRYEIELH EARIVGSYSSRFTLIDTEILERKGGEERQEPDGTQIIVRGESIKLNANQIESTGGVKNL KVSAQKVIITVSSPNEVPLYLSKFTKIDENTYEAQNVEMEIKDGGIITAEEATFTTTNF AFIRLAKVGGEVPDYMKRSLLIASLTVILTLLFVIPSAYAFSRLKFFGREHVLYFYLMF TQVAGGLGIAGLVALYGMLVKLHLTNNIFVLPVIYAAGGVPFNTWLLKSYLDSIPPDFD EAALVDGAGYLQIIRHVLIPMALPGLATVAIFAFIGGWTELILANLLLNQENYPLTVWL YTMLANLRSISWNQFAAAALVFALPVFIMFLLAQNYVRSGLTLGGLKE" ORIGIN 1 gagctcaaca cgtcgtatat cctcgtagtg agggattccg ctttcctttg taaggtctag 61 tagctttgct ggttccaagt tttagaagag ttatatatgc caagctttcg tattttgtta 121 aaccgagctt ttgaagcatt ttaattatct ttttttctct cattttttct cctcctaaag 181 atttttaata ttcactttcg acaatttaaa catcaaaggt gatacataat cattagaggt 241 gtttaacatg tataaaattt tcggatttaa agacaatgac taccttggaa aggttggaat 301 cacagagttt tctataccta agagcggttc ctatgcctac cttttaggaa attttaatgc 361 attcaatgaa ggaagcttta gaatgaggga aaagggagat agatggtata taaaggtaga 421 gcttcctgag ggaatctggt actatacatt ttcggttgat ggaaatttaa tattagattt 481 tgaaaataat gaaaaaactg tgtatagaag gctttcatat aaatttgaaa aaactgttaa 541 tgttgctaaa atcttctcgg gagagaaatt ttaccattat ccctctttgg tctatgctta 601 ttctttggga gactcaacat acattcgctt tagagccatg aagggagtgg ctaaaagagt 661 gtttttaatt tcagaccaaa aatatgaaat gagaaaaaaa gcacaagatg aactctttga 721 gtattttgaa gcagttctac ccagaaaaga aggacttgag tattattttg agattcatga 781 agcagatgaa attattgatt atggagactt taaggtagat tttaacgagc agaaggaaag 841 gtttaagcca cctgcatggg tctttgagag agtcttttat caaataatgc ccgatcgctt 901 tgctaatggg aatccagaga atgacccgca taattgtata gaatttaaaa ctatcactca 961 ccacggcggt gatcttgagg gtattataga gaaacttgac tacattgagg aactcggtgt 1021 taatgcactt tacctaaccc cgatcttcga gtcaatgaca tatcacgggt atgacatagt 1081 ggactactat catgttgctc ggaaattcgg gggagatgaa gcttttgaaa aattaatgca 1141 aaagctgaag aaacgggata taaagcttat tttagatgga gtttttcatc ataccagctt 1201 ttttcatccc tactttcaag atgtagtgaa aaatgggaaa aacagtaagt acaaagattt 1261 ttatcgtata atcagttttc cagttgttcc agaggagttt ttcgagattt taaactcgaa 1321 gctcccatgg gatgaaaaat ataggcggct taaatccctt aaatggaact acgagagctt 1381 ttattctgta tggctaatgc ctcgtttaaa ccacgacagc aaaggagtaa gagaattcat 1441 taggaatata atggagtact ggattaaaaa aggcgccgat ggctggaggt tggatgttgc 1501 tcatggagtt ccacctgaag tatgggaaga aattagagaa aagttgccca gtaatgtgta 1561 tctagttgga gaagtgatgg acgatgctag gttgtggata ttcaacaagt tccatggaac 1621 catgaactat ccattgtatg aggcaatttt gagatttttt gtaacaagag aaattaacgc 1681 agagcaattc ttgaactggc ttgaactttt gagcttctac tacggccctg ctgagtacgt 1741 gatgtataat ttcttagata accatgatgt ggatagaatg cttagcctgc ttggagacaa 1801 gagaaaatat ctctgcgcct tggtttttct tttcacgtac aagggagttc cgtctattta 1861 ctatggtaat gaaattggga tgaaaaatat agaggctcct tttatggagc gttcaagggc 1921 acctatggaa tggaataaga agaaatggga taaagaaatc cttaaaacta caaaagagtt 1981 gataaagttg agaaggagaa gcaaagcttt gcaaaaaggt atttttaaac cagttaaatt 2041 caaagacaaa ttattggtct ataaaagagt gttaaataac gaaaacatct tagtagcaat 2101 caactattca aagaaagaaa agcatctaga tttaccacct tcctttgaaa tactctttca 2161 aagtggatca ttcgacagag tgaatatccg attaaaacca ttttccagta ttattgctaa 2221 aaagttgtag ctttctgtcc ttttttgaaa ctttttgtta agtctcataa tgatcactgt 2281 acattttccg tatccgtact taattacata tagttataca ttagtggaca gcttcgtaaa 2341 atttataata atttttgtat atcattaata gtgatacatt attaatagtg atacattttt 2401 ggagggagat gacatgagaa gcagaaagat ttatatgctt gttgtattgc ttcttttttt 2461 aggatttagt gaacaaattt caacagtagc tgcaggggtc agtccctcat atccagcagg 2521 ggatccccag acatgggtta tctaccaaat agtcattgat agattttacg atgggaacac 2581 ctcaaacaat gatccattaa aaagtccagg attatatgat ccaaacaaag agaactggaa 2641 gctttattgg ggcggtgatt tagagggcat catagcaaag ctgccctact tatatgaact 2701 tggagtgagt gccatatgga tttctccagt gtttgataat atagatgttc ctatcaatgg 2761 aagcaatggt ctagaggcgg ggtatcatgg gtattggcca aaggacttta aggttataga 2821 agagcacttt gggacatggg aaatattccg cagacttagt caagaagcag ccaagtacaa 2881 tatcacgatt ataattgact tcgttccaaa ccacagcaat cccaatgatg cgggcgagta 2941 tggagctcta tatgataatg gcacgtttgt tattgattat ccaacggatg caaattatgc 3001 gactgttcac ccaataacaa agagtttatc gtacatatac aatcacaatg gaggaataac 3061 aaattggaat gatagatggg aagtcagata taaaaatctc tttaatctgg ctgatcttaa 3121 tcagttgaat ccatgggtgg acaattatct taaagaatcg acagtatctt acttagaagc 3181 tggaattgga ggaataagaa tagatgcagt taaacactta gagcctggat ggctcaaaac 3241 ttatgcagac tatgtatatg caagaaagaa cgtatttatg tttggagaat ggtaccaggg 3301 ctttaacgat gagatgtact gggatatggt taagtttgca aactatacgg ggataggcct 3361 tattaacatc ccactccaac aagttctcgt tgatgttttt gcttatgata caaagactat 3421 gtgggacttg gaaagcgctg ttaataaata taccattgac tttatgtggc aaaataaact 3481 aactatattc gtagacagcc acgatgttcc aagatttctg tccttgagaa atgacttaat 3541 aagatttcac caagctcttg cttttgttct aaccgctcct ggaattccaa taatctatta 3601 cggtgatgaa caatatcttc acaatgatac tgtaaatgac tttggtcaag ttggaggtga 3661 cccatataat agacctatga tgaaaacttg gaacacatca acaaccgctt ttaaactaat 3721 aaagactctc gcaagtctga gacgctataa tcctgcttta gcttatggat tgatcgcgac 3781 aaggtatgta agcgatgaca tttacatctt tgaaagaaag ttctttgata acgttgttct 3841 cgtagcaata aatcgcaatt taaattctcc atatgtggtc tctaacgttt acacctccct 3901 accagatgga agttacaatg actaccttgg aggacttttg aacggagttg gaataacggt 3961 ttcatctggt acattctctg tagagttacc tgcaggttca gtatcagtat ggcagtacaa 4021 agcaacgccg actgatccat gggtaggagc aattgatcca gttatgggaa gagctggaaa 4081 cattgtcaca gttagtggag aagggtttgg ggatgttccg ggaagagtct taataacaaa 4141 tggacaagat tactggactg cagaagttac atattggagc gataaaagcg tagagttcat 4201 agttccttca ggaataacta cccaactaaa cgaaaatcat gttgaagtca gaattgaaag 4261 ggctgatggg gctacatcaa atggaatagc ctttgaatat ctaacgaaca aacaaattcc 4321 agctattttt gaagttagga acactcaagg tacaaattta gaaacccaag ttggagaatt 4381 tttatggcta acaggtagtg ttccagagtt gagctattgg agtccagaaa caataaaagc 4441 tgttggacca atgttgtgtc caggatggcc tgattggttt gttgtagcaa gtgtcccggc 4501 ggatacctat atagagttca agttcttaaa ggcaccgctt ggaggcactg gaatctggga 4561 agtaggaagc aatcacgcat acctaacacc ttcaagcgga ataggggaag tttcagttga 4621 agccaaccgt tgatattctt ttttattatt cttattgcat ctatttgaca ttattgtatt 4681 gtttagttca tatgtcaatt cgtgagcaat gaagcgtaag gtatatatat cactgaggat 4741 gatactaaat ttgaaatatt caactgaggt gaatatgcat gaagaaggca ctgtttgctt 4801 tattgttgat tggagtgctg gcaattggag tagttgcaag tgggtgtatt ggtggtgggg 4861 ggactacttc aagccccaca caaacaacac aaaccacaac cacaagctcc ccaacccaaa 4921 caaccacaac tacgagctct cctactcaga caacaaccac aacaacagaa gagaagaaag 4981 taacaatcgt tatatggcat gcaattggtc cgaatgagct caaggctttt gaagatttga 5041 ttgccgagtt tgagattgag tatccaaaca tcgatattca acttgaacaa aaggctgatt 5101 tggagacttc ccttaaggct gcaattcccg ctggacaggg gcccgacctt ttcatgtggg 5161 ctcatgattg gattggaaag tttgctgagg caggtcttct tgagccgatt gacgagtaca 5221 taacccccga aattctaaac aagttcagtc caatggctca aaatgctatt gagtacggtg 5281 ggcattatta tgcgatgcct tatgctgctg agactgtagc tttaatttac aacaaggaca 5341 tggttccaaa cccaccaaag acctttgacg aaatgaaggc aattatggaa aagtactaca 5401 atgaagctga aggtacttac ggtctcgcaa ctccaatgga cccatacttc ctttcagggt 5461 gggttcacgc cttcggtggc tactactttg acgataagac aaagactcct ggccttgata 5521 agcctgagac acttcaaggc tttaagttct tctttgagca aatattccca tatgttgcca 5581 agactcaaga ctacaacgct caggttagcc tcttccacga tggaaaggct ccaatgatga 5641 ttaacggtcc atggagcatt ccagacgtta gaaacgctgg aatcaacttt ggcatagttc 5701 cacttccacc tattgatgat aagcacaggc ctcaccccta cggtggagtt aggcttatct 5761 atgttgcaaa gctcaacgac ccaagcaaga aagatgccat atggacgttc ctcaagtggt 5821 tcacaacaaa cccagacgtt ataaagactc tctccctcca aaacggttac atcccagtac 5881 ttaccgaagt ccttaacgac ccagagattc aaaatgaccc agttctttac ggctttggtc 5941 aagcagtcca atacgcaatt ccaatgccaa agagcccaga gatgggtgcc gtttggggtc 6001 cggttggaac tgcaatcacg gacataatcg ctgggaagaa aacaatcgag caagcacttc 6061 aagacgcaca gaaagaaata cttgatgcca ttagcggcag ctgatttttc tttttctttc 6121 taactttcta attcttggag gtgtgagtaa atggacaaaa aaacaatagc cgctctcgct 6181 ttaatattgc ccggcatgat agcgttcttg ttttttaaca tctatccaat cttgtactcc 6241 atttatattg cgttcacaaa tgccaagtta ggtaatttcc caatacaggc tcctactgcc 6301 gaaccgttaa ggtttgtcgg gttggaaaac tttaggtggg cgttaagcga tccaaagttc 6361 agatctgctt ttgtgtggac ttgggttttt gtggcaacta gtgtgacttt gaaggtagtt 6421 gttggaattc ttttgagtgt tctgtataat aacaaatacg taaagggcaa gtttttctat 6481 agagcccttt tgataattcc atgggcactg cccttgcttt tttccgtgat ggtatggcgc 6541 tttatgtttg atcccgttgt tggtcctatt aatataatgt taagagactt aggaataaca 6601 aacccaccag actggatgac gagtgtaact tggggctttg ttgctttaaa cataattgaa 6661 gtgtggcttg catatccgtt tatgatgacg gtaataactt cggccctcca gagtgttcca 6721 gatacgctca ttgaggcagc gattattgat ggagcaaact actggcagag attgacgaag 6781 gtggttatcc caattgtcag caagcctatt gcttttgcca caattctaac ttccgcggca 6841 agctttcagt acttccttgt ccccttcatt tacaacgcag gtctttttga agacagattt 6901 ctgctccttt atggatatag aaaagctttc ggcagttcag ttcctcacta cggaagagcg 6961 gcagccattt tgctcatagc aacaatagtc ctcgcagttt acatgttcgt caacatgagg 7021 ataacgagac tgcaggaggg ggttaaggga tgaaacttaa agttgaacta cccaaacgga 7081 aagatgagct attaaagtca tttgtgctga ctttattggc actttttgtt ttggcaattc 7141 tgctcttccc cgtgtactac atggcaatgc tctctgttaa gccttcggga actcttgcta 7201 ctactgaaat tgatctaatt ccggataaag tgactttctc caattaccgg gatctactct 7261 ttggtcatac tgaggggctg ataaaaacaa caaactttga aataaatgct aaaaaaggaa 7321 ctataaagga ttccctaaac agatatgaga tagaacttca tgaggctagg attgtaggaa 7381 gttattcgag taggttcacc ttgatagata cagaaatact cgaaagaaaa ggtggagagg 7441 agagacaaga acctgatggg actcaaatta ttgtcagagg tgaatcgata aaattaaatg 7501 ccaatcagat tgaaagtacc ggcggtgtta aaaacctcaa agtatctgca caaaaggtaa 7561 taataactgt cagttctccc aatgaagtac ctttatatct ttcaaagttc accaaaattg 7621 atgaaaacac ctatgaagcc cagaacgtgg aaatggagat aaaagacgga ggaataataa 7681 cagctgagga ggcaacattc accacgacta actttgcatt tattcgctta gcaaaagtag 7741 gtggagaagt cccagattac atgaagagaa gtctactgat agccagtctc accgttatct 7801 tgacgctctt atttgtgata ccctctgcat atgcattttc aagacttaag ttctttggga 7861 gagagcacgt cctatacttt tacttgatgt tcactcaggt cgcaggagga ctagggatag 7921 caggattggt tgcattgtac ggtatgttag tgaagctcca cttgaccaat aacatctttg 7981 ttttgccagt aatctatgcc gctggtggag tgccgtttaa cacatggctc ttaaagtcct 8041 acttggattc aattcctccg gattttgacg aagcagcatt ggttgatggt gcgggatacc 8101 tgcagattat aaggcacgtg cttattccaa tggctctccc cggattggca acagtggcaa 8161 tatttgcatt catcggaggt tggacagagc ttatactcgc taacttgctc cttaaccaag 8221 agaactatcc tctaactgta tggttataca caatgctagc gaatttgagg tctatctcat 8281 ggaaccaatt tgccgcggcc gcgttggtat tcgcattacc cgtgtttata atgttcctct 8341 tggctcaaaa ctatgtaaga agtgggctta ctcttggagg attaaaggaa tgaggtgata //