LOCUS CP000303 7061 bp DNA linear BCT 31-JAN-2014 DEFINITION Bifidobacterium breve UCC2003, complete genome. ACCESSION CP000303 VERSION CP000303.1 DBLINK BioProject: PRJNA13487 BioSample: SAMN02604112 KEYWORDS . SOURCE Bifidobacterium breve UCC2003 ORGANISM Bifidobacterium breve UCC2003 Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium. REFERENCE 1 (bases 1 to 7061) AUTHORS O'Connell Motherway,M., Zomer,A., Leahy,S.C., Reunanen,J., Bottacini,F., Claesson,M.J., O'Brien,F., Flynn,K., Casey,P.G., Moreno Munoz,J.A., Kearney,B., Houston,A.M., O'Mahony,C., Higgins,D.G., Shanahan,F., Palva,A., de Vos,W.M., Fitzgerald,G.F., Ventura,M., O'Toole,P.W. and van Sinderen,D. TITLE Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor JOURNAL Proc. Natl. Acad. Sci. U.S.A. (2011) In press PUBMED 21690406 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 7061) AUTHORS O'Connell Motherway,M., Zomer,A., Leahy,S., Reunanen,J., Bottacini,F., Claesson,M., O'Brien,F., O'Flynn,K., Casey,P., Moreno-Munoz,J., Higgins,D.G., Shanahan,F., de Vos,W., Fitzgerald,G.F., Ventura,M., O'Toole,P. and van Sinderen,D. TITLE Direct Submission JOURNAL Submitted (27-MAR-2006) Department of Microbiology, Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Munster, Ireland COMMENT Source DNA or strain is available from Douwe van Sinderen, d.vansinderen@ucc.ie. FEATURES Location/Qualifiers source 1..7061 /organism="Bifidobacterium breve UCC2003" /mol_type="genomic DNA" /strain="UCC2003" /db_xref="taxon:326426" gene <1..8 /locus_tag="Bbr_0525" CDS <1..8 /locus_tag="Bbr_0525" /EC_number="4.2.1.70" /codon_start=2 /transl_table=11 /product="Ribosomal large subunit pseudouridine synthase D" /protein_id="ABE95222.1" /translation="MTWISPATSPSPSPLSKPHRYVIDEPHIPFEMDVLYEDERIIVVD KPHFLATMPRGMWYRETALIRLRERLGEPDITPAHRLDRMTAGVLVFVRDPACRGAYQM LFQNKQATKVYECLASCAPIRRPRFGTITQAEPPRPFPLLRRSHITKERGTLAAFEEPG AVNAETLIERGELILTSAGGRWREAPEGGPCLENPPICRYTLHPRTGKTHQLRVHMNSL GLPIVGDDFYPRIQTRLYDDFSQPLQLVARVLRFTDPVTGKRQEFVSRIPLIS" gene complement(1..1011) /locus_tag="Bbr_0526" CDS complement(1..1011) /locus_tag="Bbr_0526" /codon_start=1 /transl_table=11 /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955" /protein_id="ABE95223.1" /translation="MARPTQVSMQDVAKAAGVSPQTVSRVANDSNAVKPETRQRVEAAM ERLGYRPNYAARALKHGRFQDIGVVMFDTTRFGNARILDSIVTHAAEDGYSVTIQTMSN GAERTLASAIDRMQRQPVDGVIVVLEERISDFVGYKPPRELPVVLISEAAADHCPTVDA DQYGCSTAIVDYLMSKGHKTVYHIAGPVTSRAAESRAQGWREALEQIGARVPSMYIGDW CADSGYQAGVALAHDPECTAIYAANDQMAYGAMLGLESAGKRVPEDVSIVGVDDSLVDV VPRLNLTTMRMRNDAIGATAFSMVRRQCEGEKVPAGIKTVIPTELIKRGSVRDIS" gene 1227..2153 /locus_tag="Bbr_0527" CDS 1227..2153 /locus_tag="Bbr_0527" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|D6ZW63|3.A.1.1.48" /protein_id="ABE95224.1" /translation="MASADVAAVPMHTDKARENKHKADWRGWKFMWPFTVVFVFVFIIP IVYAVYISFFKKQMIGGTRFVGVENYARLLQDGQFWSSVGRVALFTCVQVPIMLFLSAA MALALDSMKLHGAKFFRISTFLPYAVPAVVSTLVWGFVYGAKYGLVGSFNDFFGTNVDV LQPGVLLASIGNISTWEFTGYNMLIFYSSLSTIPHSLYEAASIDGASEWQIVKSIKLPE LKGSLAITVIFSIIGSFQLFNEPSILQNMVPGNAITTYYTPNMYAYNLSFSGNQSNYAA ALAIVMAVITMAIAYAVQLKSMKEQMK" gene 2206..3204 /locus_tag="Bbr_0528" CDS 2206..3204 /locus_tag="Bbr_0528" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|D6ZW62|3.A.1.1.48" /protein_id="ABE95225.1" /translation="MSAAASTRLTDAELKAREKAARKAEKERQKRIAADEAAERKRSAR AGFANVNNPRRSTLMTVLCAIFAVYCLFPFVYLVINATKTQADFTSTFGLGFGKSFALW DNIVTVFTYQGGIFGRWLVNTLLYVVVGAGGATLLAIMGGYALAKFRFPGRKAVFAVII GSISVPGIALAVPQFLLFAKLGLTNTPWAMIIPSLISPFGLYLMWIFSEQAVPTELLEA ARVDGASEFRTFWTISLPLLAPGIVTTALFTIVATWNNYFLPLIMLKDADWYPLTIGLN QWKDQASTAGGQAIQNLVITGSLITIIPLVIAFLCLQKYWQSGLAAGAVKE" gene 3384..5459 /gene="lacZ5" /locus_tag="Bbr_0529" CDS 3384..5459 /gene="lacZ5" /locus_tag="Bbr_0529" /EC_number="3.2.1.23" /codon_start=1 /transl_table=11 /product="GH42" /protein_id="ABE95226.1" /translation="MEHREFKWPQPLAGGKPRIWYGGDYNPDQWPEEVWDEDVALMQKA GVNLVSVAIFSWAKLEPEEGVYDFDWLDRVIDKLGKAGIAVDLASGTASPPMWMTQAHP EILWVDYRGDVCQPGARQHWRATSPVFLDYALNLCRKMAEHYKDNPYVVSWHVSNEYGC HNRFDYSEDAERAFQKWCEKKYGTIDAVNDAWGTAFWAQRMNNFSEIIPPRFIGDGNFM NPGKLLDWKRFSSDALLDFYKAERDALLEIAPKPQTTNFMVSAGGAGIDYDKWGYDVDF VSNDHYFTPGEAHFDELAYSASLCDGIARKNPWFLMEHSSSAVNWRPINYRVEPGELVR DSLAHLAMGADAICYFQWRQSKAGAEKWHSSMVPHAGPDSQIFRDVCELGADLNKLADE GLLSTKLVKSKVAVVFDYESQWATEHTATPTQEVRHWTEPLAWFRALADNGLTADVVPV RGPWDEYEAVVLPSLTILSEETTRRVREYVANGGKLFVTYYTGLVDDKDHVWLGGYPGS IRDVVGVRVEEFAPMGNDFPGAMDHLDLDNGTVAHDFADVITSVADTAHVVASFKADKW TGFDGAPAITINDFGDGKAAYVGARLGREGLAKSLPALLEELGIETSAEDDRGEVLRVE RADETGENHFVFLFNRTHDVAIVDVDGEPLVASLAQVNESEHTAAIQPNGVLVVKL" gene 5715..7061 /locus_tag="Bbr_0530" CDS 5715..7061 /locus_tag="Bbr_0530" /codon_start=1 /transl_table=11 /product="gnl|TC-DB|D6ZW64|3.A.1.1.48" /protein_id="ABE95227.1" /translation="MKFTTAKKAVALTGAVAMLVSVAACGSDSGKSSQPAQDSDVKEIT VWAWEPSLTQVAKDFEKETGIKVDLKNVGTNTKEYTQLDNAIEAGSGAPDVAQVEYYAV PQYAIKGNLLDITDKTSGYKDFYTPGPWASVQFAGKVYGLPMDSGPMAFFYNKEVFDKA GVDAEQIKTWDQYYDAAKKIHALGDNYYITSDTGDAGFFDSMTWLAGAKPFQTSSDGSE VTVNLTEDKGVKTFTDFWQKLLDEGLLDTKTAGWSEDWFKGMVDGTIASLFTGAWMPAN LANSAADGAGKWRVTQMPTADGSTTNSENGGSSLAVLASTKKADAAYQFIEYANHGAGV ATRVAGGAFPADKASLEKDSFKNATTVKNADGQDVDYFGGQKYNEVLAQAAENVSSGYQ FLPFEVKARTIFGDYFGKSYTGDQKLSDGVAAWQKALQDYGKDQGFTVK" ORIGIN 1 tcagctaatg tcgcggacgg aaccgcgctt gatgagttcg gtggggatta cggttttgat 61 gccggccggt actttctcgc cttcgcattg acggcggacc atggagaacg cggtggcgcc 121 gatggcgtca ttgcgcatgc gcatggtggt gaggttgagt cttggcacca cgtcgaccag 181 cgaatcgtcc acgcccacaa tgcttacgtc ttccggcacg cgcttgccgg ctgattccaa 241 tccgagcatc gcaccgtagg ccatctgatc gttggccgca tagattgcgg tgcattccgg 301 gtcgtgagcc agcgccacgc ctgcctgata gccggagtcg gcacaccagt cgccgatata 361 catgctcggt acgcgcgcgc ctatttgttc caaggcttca cgccagcctt gcgctcggct 421 ttcggcagca cgtgaagtga ctgggccggc gatatggtag acggtcttat ggcctttgct 481 catcaaatag tcgacgatgg ctgtggagca tccgtattga tcggcgtcaa cggtggggca 541 gtggtcggct gccgcctcgg aaatcagcac aaccggtagt tctcgcggtg gtttgtatcc 601 cacgaagtcg gaaatgcgtt cctcaagcac cacgatgacg ccatctacgg gttgacgttg 661 catgcggtcg atggcggagg ccagcgtacg ttctgcgccg ttgctcatag tctgaatggt 721 gacggaatag ccgtcctcgg ctgcatgggt gacaatggaa tcgagtatcc gggcattacc 781 gaacctggtg gtgtcgaaca tcactacgcc gatatcctgg aatcgcccat gcttcaatgc 841 tcgggcggcg taattcggac gatatccgag gcgttccatc gcggcttcaa cgcgctggcg 901 ggtttccggc ttgaccgcat tgctgtcatt cgccacacgg gatacggttt gcggcgaaac 961 gccggccgcc ttagccacat cttgcatgga aacctgtgtt ggtctcgcca tctcgcccct 1021 cgcttgtctc tcaaacgaca gcggtcatac cgctgcgatg tctaccatgt atcgcgtgaa 1081 cggtaacgat aacatcattg ttttcgtgac cattgtaaca gctagcgcaa aaacattgaa 1141 ttcaacacga ttggacaatg ttatgaatgg tgttatgatg ataacgtaaa caattttggt 1201 caaccttcga aggagagggg ttggaaatgg cttcagccga tgtcgctgcc gtgcctatgc 1261 acacggataa agcgcgtgag aacaagcata aggccgattg gcgtggatgg aagttcatgt 1321 ggccgttcac ggtggtgttc gtgttcgtgt tcattattcc gattgtgtac gccgtttaca 1381 tcagcttctt caagaagcag atgatcggcg gcacgaggtt cgtcggggtc gagaactacg 1441 cgcgtctgct gcaggacggc cagttctggt cgtccgtggg tcgtgtggcg ctgttcacct 1501 gcgtgcaggt gccgatcatg ctgttcctgt cggctgccat ggccctcgcc ctggattcga 1561 tgaagctgca cggcgccaag ttcttccgca tcagcacgtt cctgccctat gcggtgcccg 1621 cagtggtgtc cacgttggtg tggggcttcg tgtacggcgc gaagtacggc ctggtgggct 1681 cgttcaacga cttcttcggc acgaacgtcg acgtgctgca gccgggcgtg ctgcttgcct 1741 ccatcggcaa catcagcacg tgggagttca ccggctacaa catgctgatc ttctactcgt 1801 cgctgtccac catcccgcac tccctgtacg aggccgcctc catcgacggc gcctccgaat 1861 ggcagatcgt caagtccatc aagctgcccg agctgaaggg ctcgctggcc atcacggtga 1921 tcttctcgat catcggcagc ttccagctgt tcaacgagcc gagcatcttg cagaacatgg 1981 ttccgggcaa cgcgatcacc acgtactaca cgccgaacat gtacgcgtac aacctgagct 2041 tctccggcaa ccagtcgaat tacgcggccg ctctggccat cgtgatggcc gtcatcacca 2101 tggccatcgc ctacgccgtg cagctgaaga gcatgaagga gcagatgaag tgacgacgac 2161 cgcccgtatt gccggattgg tttcgattgt gaaggagcgt gcgtgatgag tgccgcagca 2221 tctacccgtc tgactgacgc cgaactgaag gcgcgggaga aggccgccag gaaggccgag 2281 aaggaacgcc agaagcgcat cgccgccgat gaggcggccg agcgcaagcg cagcgccagg 2341 gccggtttcg cgaacgtgaa caacccgcgc cgttcgaccc tgatgaccgt gctgtgcgcg 2401 atcttcgccg tgtactgcct gttcccgttc gtgtacctgg tgatcaacgc caccaagacc 2461 caggccgact tcacctccac gttcggcctc ggcttcggca agagcttcgc cctgtgggac 2521 aacatcgtca cggtgttcac ctaccagggc ggcatcttcg gccgctggct ggtcaacacc 2581 ctgctctacg tggtcgtcgg cgccggcggc gccaccctcc tggccatcat gggcggctac 2641 gcgctggcca agttccgctt ccccggccgc aaggccgtgt tcgccgtgat catcggctcg 2701 atctccgtgc cgggcatcgc cctggccgtg ccgcagttcc tcctgttcgc caagctgggc 2761 ctgaccaaca ccccgtgggc catgatcatc ccgtcgctga tctccccgtt cggcctgtac 2821 ctgatgtgga tcttcagcga gcaggccgtg cccaccgagc tcctggaggc cgcccgcgtg 2881 gacggcgcgt ccgagttccg cacgttctgg accatcagcc tgcccctttt ggcccccggt 2941 atcgtgacga ccgccctgtt cacgatcgtg gcgacgtgga acaactactt cctgccgttg 3001 atcatgttga aggacgccga ctggtacccg ctgaccatcg gtctgaacca gtggaaggac 3061 caggcctcga ccgcaggcgg ccaggccatc cagaacctgg tgatcaccgg ctccctgatc 3121 acgatcatcc cgctggtcat cgcgttcctg tgcctgcaga agtactggca gtccggcctc 3181 gccgcaggcg ccgtcaagga gtaaccgtac ttcggctccc ttctggtgga gggagccgag 3241 ataaaggcat aaccactaac aggaaggagg cgtctatgca gatattcgca ttgttgagaa 3301 tcgtgcaggc gcctccttgt gtatctgcgt gactcccatt tgcccacacg tagatattta 3361 ttcattaaag gaacagcatc atcatggaac atcgcgaatt caagtggccg cagcctcttg 3421 cgggtggcaa gccccgcatc tggtacggcg gcgattacaa ccccgaccaa tggcctgagg 3481 aagtgtggga cgaagatgta gccctcatgc agaaggccgg cgtcaacctc gtctccgtag 3541 ccatcttctc ttgggccaag cttgagcccg aagaaggcgt gtatgacttc gattggctcg 3601 accgcgtcat cgacaagctc ggcaaggccg gcatcgccgt cgatctcgcc tccggcaccg 3661 catccccgcc gatgtggatg acccaggccc acccggagat cctctgggtc gactaccgcg 3721 gcgacgtctg ccagcccggt gcccgacagc actggcgggc caccagcccg gtcttccttg 3781 actacgcgct caacctgtgt cgcaagatgg ccgagcacta caaggacaac ccctatgtgg 3841 tctcttggca cgtgagcaac gagtacggct gccacaaccg cttcgactac tccgaagatg 3901 ctgagcgcgc cttccagaag tggtgcgaga agaagtacgg caccatcgac gccgtcaatg 3961 acgcctgggg caccgccttc tgggcgcagc gcatgaacaa cttctccgag atcatcccgc 4021 cgcgcttcat cggcgatggc aacttcatga acccgggcaa actgcttgac tggaagcgct 4081 tcagctccga cgccctgctc gacttttaca aggccgagcg cgacgccctg ctcgagatcg 4141 cccccaagcc gcagaccacc aacttcatgg tctccgccgg cggtgccggt atcgattacg 4201 acaagtgggg ttacgacgtg gacttcgtgt ccaacgatca ctacttcact cctggcgaag 4261 ctcacttcga tgagctggcc tactcggcct ccctgtgtga cggcatcgcc cgcaagaatc 4321 cgtggttcct catggagcac tccagctccg ccgtcaactg gcgtccgatc aactaccgcg 4381 tcgaacccgg cgaactggtg cgcgactccc tggcccacct ggccatgggc gccgacgcca 4441 tctgctactt ccagtggcgc cagtccaagg ccggtgccga gaagtggcac tcctcgatgg 4501 ttcctcacgc gggccccgac tcccagatct tccgcgacgt gtgcgagctg ggtgccgacc 4561 tcaacaagct tgctgacgag ggcctgctga gcaccaagct ggtcaagtcc aaggtcgccg 4621 tcgtcttcga ttacgagtct cagtgggcta cggaacacac cgccacgcct actcaggagg 4681 tacgccattg gaccgagccg ctcgcgtggt tccgcgcgct ggcggacaac ggtctgaccg 4741 cagacgtggt gccggttcgc ggtccctggg acgagtacga agccgtcgtg ctgccgagcc 4801 tgaccatcct gtctgaagag accacgcgcc gcgtgcgcga gtatgtggcg aacggcggca 4861 agctgttcgt gacctactac accggtctgg tggacgacaa ggatcatgtc tggctgggtg 4921 gctaccccgg ctccatccgt gacgtggtgg gcgtgcgcgt tgaggaattc gccccgatgg 4981 gcaacgactt ccccggtgcc atggaccacc tcgacttgga caacgggacc gtggcgcacg 5041 atttcgccga cgtgatcacc tccgtggctg ataccgctca cgtggttgct tccttcaagg 5101 ctgataagtg gaccggtttc gacggcgctc ccgccatcac catcaacgac ttcggcgacg 5161 gcaaggccgc atacgtcggt gcccgtctcg gccgtgaggg cttggccaag agcctgcccg 5221 cgctgctgga ggaactcggc atcgagacct cggccgaaga cgatcgcggc gaagtgctgc 5281 gcgttgagcg tgcggatgaa actggcgaga accacttcgt gttcctgttc aaccgcaccc 5341 acgatgtggc gattgtggac gtggacggcg agccgctggt cgcctcgctg gctcaggtca 5401 acgaatccga gcatacggcg gccattcagc cgaacggtgt gctggtggta aagctgtaaa 5461 gaggtctatt tatagcgatt caaatgcgat ggcgggcgtg ttagcgataa cacgcccgcc 5521 atcgcatttg cgatttgttt tgagatgtgt atactaaaag atgttatcgc aaccatagcg 5581 aaaggaatgg cttcatagag ccacttctca gtgtcgagga gaaatcaata attgacacta 5641 ggtaacacgc aatgattgcg agcgacaaaa cagtttgaat cgttctagac gattcgagga 5701 aggagaacag aacgatgaag ttcactaccg ccaagaaggc cgttgccctc accggtgcgg 5761 ttgccatgct ggtttccgtt gccgcctgcg gctctgatag cggcaagtcc agccagccgg 5821 ctcaggattc cgacgtcaag gaaatcaccg tctgggcttg ggagccttcg ctgacccagg 5881 ttgccaagga cttcgagaag gagaccggca tcaaggtcga cctgaagaac gtcggtacca 5941 acaccaagga atacactcag ctggacaacg ccattgaggc tggctctggc gctccggacg 6001 tcgctcaggt tgagtactac gccgtcccgc agtacgccat caagggcaac ctgctggaca 6061 tcaccgacaa gacctctggt tacaaggact tctacacccc tggcccgtgg gcttctgtgc 6121 agttcgccgg caaggtctac ggcctgccga tggattccgg cccaatggcc ttcttctaca 6181 acaaggaagt cttcgacaag gccggcgttg atgccgagca gatcaagacc tgggatcagt 6241 actacgacgc cgccaagaag attcacgccc tcggcgacaa ctactacatc acctccgaca 6301 ccggcgacgc cggcttcttc gactccatga cctggctggc cggcgccaag ccgttccaga 6361 cctcctccga tggttccgaa gtcaccgtca acctgaccga agacaagggc gtcaagacct 6421 tcaccgactt ctggcagaag ctgctggacg agggtctgct cgacaccaag accgccggct 6481 ggtccgagga ttggttcaag ggcatggtcg acggcaccat cgcctccctg ttcactggcg 6541 cttggatgcc tgccaacctt gctaactccg ctgctgacgg tgccggcaag tggcgtgtga 6601 cccagatgcc gaccgctgac ggctccacca ccaactctga gaacggtggt tcttcgctgg 6661 ctgtgctcgc ctccaccaag aaggctgatg ctgcttacca gttcatcgag tacgccaacc 6721 acggtgctgg tgtggccact cgtgtggctg gcggcgcctt cccggctgac aaggcctccc 6781 tggaaaagga ctccttcaag aacgccacca ccgtgaagaa cgccgatggt caggatgttg 6841 actacttcgg tggtcagaag tacaacgagg ttctggctca ggctgctgag aacgtgtcct 6901 ccggctacca gttcctgccc ttcgaggtca aggcccgcac catcttcggc gactactttg 6961 gcaagtccta caccggtgac cagaagctga gcgacggtgt cgctgcttgg cagaaggccc 7021 tgcaggatta cggcaaggat cagggcttca ccgtgaagta a //