LOCUS NC_010999 11193 bp DNA linear CON 17-FEB-2020 DEFINITION Lactobacillus casei BL23, complete sequence. ACCESSION NC_010999 VERSION NC_010999.1 DBLINK BioProject: PRJNA224116 BioSample: SAMEA2272724 Assembly: GCF_000026485.1 KEYWORDS RefSeq; complete genome. SOURCE Lactobacillus casei BL23 ORGANISM Lactobacillus casei BL23 Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus. REFERENCE 1 AUTHORS Maze,A., Boel,G., Zuniga,M., Bourand,A., Loux,V., Yebra,M.J., Monedero,V., Correia,K., Jacques,N., Beaufils,S., Poncet,S., Joyet,P., Milohanic,E., Casaregola,S., Auffray,Y., Perez-Martinez,G., Gibrat,J.F., Zagorec,M., Francke,C., Hartke,A. and Deutscher,J. TITLE Complete genome sequence of the probiotic Lactobacillus casei strain BL23 JOURNAL J. Bacteriol. 192 (10), 2647-2648 (2010) PUBMED 20348264 REFERENCE 2 (bases 1 to 11193) AUTHORS Loux,V. TITLE Direct Submission JOURNAL Submitted (18-JUN-2008) Loux V., Unite MIG, I.N.R.A, Domaine de Vilvert, 78352 Jouy en Josas Cedex, FRANCE COMMENT ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 02/17/2020 14:41:02 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Method :: Best-placed reference protein Annotation Software revision :: 4.11 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; Genes (total) :: 3,020 CDSs (total) :: 2,941 Genes (coding) :: 2,878 CDSs (with protein) :: 2,878 Genes (RNA) :: 79 rRNAs :: 5, 5, 5 (5S, 16S, 23S) complete rRNAs :: 5, 5, 5 (5S, 16S, 23S) tRNAs :: 61 ncRNAs :: 3 Pseudo Genes (total) :: 63 CDSs (without protein) :: 63 Pseudo Genes (ambiguous residues) :: 0 of 63 Pseudo Genes (frameshifted) :: 30 of 63 Pseudo Genes (incomplete) :: 24 of 63 Pseudo Genes (internal stop) :: 13 of 63 Pseudo Genes (multiple problems) :: 4 of 63 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## REFSEQ INFORMATION: The reference sequence was derived from FM177140. Source DNA and bacteria available from Josef Deutscher (josef.deutscher@grignon.inra.fr) or Manuel Zuniga (btcman@iata.csic.es) Finishing done by Lactobacillus casei BL23 sequencing consortium Automatic Annotation done by INRA-MIG with Agmial URL http://genome.jouy.inra.fr/agmial. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline (PGAP) set; GeneMarkS-2+ repeat_region COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..11193 /organism="Lactobacillus casei BL23" /mol_type="genomic DNA" /strain="BL23" /db_xref="taxon:543734" gene 1..1710 /locus_tag="LCABL_RS01420" /old_locus_tag="LCABL_02860" CDS 1..1710 /locus_tag="LCABL_RS01420" /old_locus_tag="LCABL_02860" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003563123.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH20" /protein_id="WP_012490882.1" /translation="MKKHGFRFWLLAIVGLLGMVLSLSQPPKQVAAADNTLKSVFSIDA GRKFFSADQLKMIIDRAHTDGYTDVQVLLGNDALRLLLDDMSVTINGKTYGSDVVKQAI QAGNKAYYDDPNGNALTQTDMDAVLKYAAARDINIIPVINSPGHMDAILTAMAQLGIKN PAFNGSKRTVDLNNDTAIAFTKALLQKYVMYFKGHATIFNFGSDEYANDVDTGGWAKLQ QSGTYKKFVAYVNDLAAMAKNASLKPMVFNDGIYYDNNTSFGTFDKDLIVSYWTAGWGG YDVAKPEFLTDKGLKIMNTNDGWYWVLGRVDGDLYSYKTALASLASKKFTDVPGASSAV PIIGSVQAVWADDPSAQLDMPALLKLMDQFSTAYAPYLVRPADYSKVDAAIAAVPRQLN QYTEASVAKLDAALNAVVRGKKATDQALVDGYAQTITVAIKALQLRPADYTKVDAAIAA AKKLDRSHYQDLSAVDAALAAVNRNLSITQQAQADTMAAKITAAIAALVLKPAPQPDPR QQQVPTKTIVNPDRYLPKTAEASGWELMLAGLATIFGVISIVFFWRQHRMAV" gene 2091..3110 /gene="manA" /locus_tag="LCABL_RS01425" /old_locus_tag="LCABL_02870" CDS 2091..3110 /gene="manA" /locus_tag="LCABL_RS01425" /old_locus_tag="LCABL_02870" /EC_number="5.3.1.8" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016388442.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="mannose-6-phosphate isomerase, class I" /protein_id="WP_003592893.1" /translation="MRISDLKRLFKKGVGILTEPLFLKPYFNPKIWGGRKLETEYGFDI PDGKIGEAWIISGHPHGPATIENGPLKGKTLREAWHEDPAYFGPQHSQEFPLLVKILDA EASLSVQVHPDDAYAAAHEAKGELGKTECWYILKADPGAYLIYGHHAKTHEQLKEMIEA GDWEHLLRKYPVKTGDFVYVPSGTIHALNKGIVALETQQSSDTTYRLYDYDRKDDQGHK RELHIKQSLDVTTVPFHDPKLDITKEQVTGGQITTFVKPPLSPHFAVYRLDVDGTMTFT QQAPYTNVTVLDGDGTFSADGKDYPIKTGDSFLIPNQIKTWAFTGKLAIVTSTPGVDA" gene 3472..4188 /locus_tag="LCABL_RS01430" /old_locus_tag="LCABL_02880" CDS 3472..4188 /locus_tag="LCABL_RS01430" /old_locus_tag="LCABL_02880" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014571081.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DBD-Pfam|GntR,DBD-SUPERFAMILY|0043758" /protein_id="WP_012490884.1" /translation="MEKVPLTKQLVDQLETFIKEKLQPNDKLPSERDLSELYQVSRNTV RAALNELFLRGFIYRSTGKGTFVAERFDERTDVSGSYSFTKQMLAMNRKPVTRIQRLEK IAAPAKIAKRMRIEPGTPVYVLDRLRIADTLPMMIERSYLPANTVPGLNQEALSKTPLY DWLEGHYGIHIVSVDEAFFAGLVTPEDAEQLRVAPASACLNIRRTTFADTGEIVEYTLS VARADQFVYHVHHVNH" gene 4257..5438 /locus_tag="LCABL_RS01435" /old_locus_tag="LCABL_02890" CDS 4257..5438 /locus_tag="LCABL_RS01435" /old_locus_tag="LCABL_02890" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003563130.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="STP|SIS,STP|SIS" /protein_id="WP_003563130.1" /translation="MFEKTTEELTKLGAQITTAEIKQQPELWQEAFDNYTNAKEKITAF LDQVTAAANGKTVRVIFTGAGSSQYVGDTVTPYLRAHGDRSKFEFESIGSTDIVSAPYD FLEADTPTILVSFARSGNSPESLKTVELAEQVVHNLHQISITCAPEGQLAKKSETEDKT LLLLQPAGSNDKGFAMTGSFSCMTLSALLVFDTASDTDKSKWVKAIAAMGQEVVAREAE VQKVLDLDFDRIVYLGSGSLGGLTRETRLKVLELTAGKYATMFDTSMGFRHGPKSFLDD KTLLFDLVSNNAYTRQYDIDVLEEVKGDGIAKSVMAVGTKQDQNFSGDAFYFENGLKDL PEAYQALPDVMFAQTVALLASIKVGNLPDTPSPTGTVNRVVKGVTLHDYAGKE" gene 5497..6657 /gene="nagA" /locus_tag="LCABL_RS01440" /old_locus_tag="LCABL_02900" CDS 5497..6657 /gene="nagA" /locus_tag="LCABL_RS01440" /old_locus_tag="LCABL_02900" /EC_number="3.5.1.25" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016379175.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="CE9" /protein_id="WP_003573361.1" /translation="MSYYIHAAKFFLKNVTEIGGYLEVTDDGKFGEYLPETAKPDGDIV EQSGKLIAPGLVDTHIHGLLGHDVMDNDWDGIETMSKNLLKAGVTSWLPTTLTGSFDQL NEVCKTIAAHAGDESGAKIQGIYFEGPYFTTKHKGAQNPKYFKNPSEQEYDAWQTSADG LLKKIAIAPEREGAADFTRYATADGTVVALGHSDATFEQAKACIEAGATVFTHTFNGMS PLSHREPGMVGAAMYLQGVDDELICDGHHVRPEVATTLIRLKGAEHIALITDCMRAGMM PDGDYVLGEFPVYVKDGMARMKDGDSLAGSVLELKDALTNLLAWNAATPEAIIRMASQT PAASCNIDDQCGSILPGRAADYLVLDADLKLEATYLDGKLGYQAEA" gene 6757..8553 /locus_tag="LCABL_RS01445" /old_locus_tag="LCABL_02910" CDS 6757..8553 /locus_tag="LCABL_RS01445" /old_locus_tag="LCABL_02910" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016365502.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|W5L187|9.B.207.2.1" /protein_id="WP_012490885.1" /translation="MTTFSIDHEFMLDGQPFKILSGAIHYFRVHPSDWYHSLYNLKALG FNTVETYVPWNLHEYNEGDFDFSGILDIERFLNTAKDLGLYAIVRPSPYICAEWEFGGF PAWLLTKKMRLRTDDPAYLQAIDRYYTALMPHLVGHQVTHGGNVIMMQVENEYGSYGED KDYLAAVAELMKKHGVDVPLFTSDGPWPATLNAGSMADAGILTTGNFGSRADMNFDRLA AFNQAHGHDWPLMCMEFWDGWFNRWGEPIIRRDPEETAEDLRAVIQRGSVNLYMFHGGT NFGFMNGTSARKDHDLPQVTSYDYDAPLNEQGNPTPKYFTIQKMIHEVLPSQAQTTPLV KPAMRQADNPLTAKVSLFSVLDQLAQPVAAPYPQTQEFLGQYTGYTLYRTNPLISGTDK GTPAKLRVIDARDRVQAFFDGKSLATQYQEAIGDDILLPEVEGRHQLDLLVENMSRVNY GSKIEAITQFKGIRTGVMVDLHFIKDYLQYPLDLNKAPQLDFTGDWQAGTPAFYQYGFD VVKPQDTYLDCRGFGKGVMLVNGVNIGRFWGKGPTLSLYVPAGLLHTGHNEVIVFETEG QYAEAINLVDHPIFKELNTEEE" gene 8556..9038 /locus_tag="LCABL_RS01450" /old_locus_tag="LCABL_02920" CDS 8556..9038 /locus_tag="LCABL_RS01450" /old_locus_tag="LCABL_02920" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003577506.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|B3W754|4.A.6.1.18" /protein_id="WP_003563136.1" /translation="MAIIAARIDGRLVHGQVANLWTTKLGATRIMVVDEAASKSDIDKS GLRMATPDGIRLSVLDVATAANHILNHKYDSQKVFIVAKRPETFLALVNAGVPITDLNV GNMSQTDETRSITKSINVVDADVDTFKELDAKGVHLTAQMVPGDTAADFMSLLKKG" gene 9051..9968 /locus_tag="LCABL_RS01455" /old_locus_tag="LCABL_02930" CDS 9051..9968 /locus_tag="LCABL_RS01455" /old_locus_tag="LCABL_02930" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003563138.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|B3W755|4.A.6.1.18" /protein_id="WP_003573365.1" /translation="MIAWWQIALLTLYAGYQILDELQIYSAMSSPVFAGLISGLVMGDM KAGLLIGASTQLMVLGVGTFGGASKIDANSGTILATAFSVSAGMKPEAAVAAIAVPVAS IMIQTDILARFSNTFFAHRIDKMVDQNNYKGIERYFLAGALPWALSRMIPVGLALAFGG GFVTDLAHVLNTSWKWLGDGLATAGAVLPAVGFAILLHYLPLKKHFPYLIMGFVFTVLF TTLFGSVQTLGTAVAGVAKGFTASFNGLSMLAMALIAFAFAAISYQQTTKLHDIAVGNG NTDAASGTAANKTNEQGEITDDEL" gene 9955..10776 /locus_tag="LCABL_RS01460" /old_locus_tag="LCABL_02940" CDS 9955..10776 /locus_tag="LCABL_RS01460" /old_locus_tag="LCABL_02940" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016387133.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|B3W756|4.A.6.1.18" /protein_id="WP_003563140.1" /translation="MMNSNKPTYKLTDKDFRQINRRSLFGFQLGWNYERMQGSGYLYTI LPQLRKIYGDGTPELKKVMKTHTQFFNTSNFFNTIITGIDLAVEEKEGIAGEETVAGIK TGLMGSFAAIGDSIFAALIPAIFGAIAAQMATQGNPVGVFIWIAAQIAVMVFRWKQLRI AYNEGVSLVTTMAHRLAALTDAATLMGVFMVGALVATMINVKIGFAPKIGSVPLDFQKY FDLIIPKLLPALIVGAVYWLLGRKHMTSTRAIFIVLIVSVLLSALGVIAKG" gene 10813..11193 /locus_tag="LCABL_RS01465" /old_locus_tag="LCABL_02950" CDS 10813..11193 /locus_tag="LCABL_RS01465" /old_locus_tag="LCABL_02950" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016386751.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|B3W757|4.A.6.1.18" /protein_id="WP_003563142.1" /translation="MSKLVLISHGQLCEELKKSAEMIMGPQDDIATVALLPNEGPEDFQ AKFKAAVEGQDDVVVLADLKGGTPNNVAARVLAEGGKFDLYSGMNLPMVISFLNAQMLD MKPDLIGDGKAGIAYINDIVKH" ORIGIN 1 ttgaagaagc acgggtttcg attttggtta ttggcgattg ttggactgct gggtatggtg 61 ttgagtctga gtcagccgcc caagcaggta gctgctgctg ataacacctt gaaaagcgtt 121 ttttcgattg atgcgggacg aaagtttttt tccgcggatc agttgaaaat gatcattgat 181 cgggcacata cagacggtta caccgatgtg caggttttat tgggcaacga tgcattgcga 241 ttgctacttg atgacatgag cgtgacgatc aatggcaaaa catatggcag tgatgtcgtg 301 aaacaggcca tacaggctgg taacaaagcg tactacgatg atccaaacgg gaatgcgttg 361 acgcaaaccg acatggatgc ggtcttgaaa tatgcagcgg cacgggatat caatatcatt 421 ccggttatca atagtcccgg ccatatggat gccattttga cggcgatggc gcaactaggc 481 attaagaatc ctgcctttaa tgggtctaaa cggactgtcg atcttaacaa tgacactgct 541 attgccttta caaaagcgct attgcagaag tatgtgatgt atttcaaggg gcatgctacg 601 atcttcaact ttggcagtga cgagtatgca aatgatgtcg atactggcgg ctgggccaag 661 ttgcaacaaa gtggcaccta caaaaagttt gtggcatacg tcaacgactt agcggcgatg 721 gccaaaaatg ccagcctgaa gccgatggtt ttcaatgacg ggatttatta tgacaataac 781 accagtttcg ggacttttga caaggatttg atcgtctctt attggaccgc tggctggggc 841 gggtatgatg tcgcaaagcc agaatttttg accgataagg gtttgaaaat catgaatacc 901 aatgacggtt ggtattgggt tttaggtcgc gtggacggcg atctctatag ttacaaaacg 961 gcgctagcta gtttagcaag taaaaaattt actgatgtac ccggcgcttc gagtgccgtg 1021 ccgattattg gcagtgtgca ggcggtttgg gcggatgatc cgagtgcaca gttagacatg 1081 ccggcgctgt tgaagttgat ggatcaattt tcgacagcct atgcacctta cttagttcgc 1141 ccagccgatt acagtaaagt tgatgccgcc atcgctgccg tgccgcggca acttaatcag 1201 tacaccgaag catcagttgc taaacttgat gcagcgttaa atgctgttgt ccgcggtaaa 1261 aaggcaaccg atcaggcatt ggttgacggc tatgcccaga ccattactgt cgccatcaag 1321 gcactgcaac tgcggccggc cgattacaca aaggttgatg ccgccattgc agctgcaaag 1381 aaacttgacc ggtcacacta tcaagacctt agtgccgttg atgcggcgct tgctgctgtt 1441 aatcgtaatc tcagcatcac ccagcaagcc caagctgata ccatggctgc caagattaca 1501 gctgccatcg ctgcgttggt tctgaagccg gcgccgcaac ccgatccaag gcagcagcaa 1561 gtgccaacaa aaaccattgt gaatccagac cggtatttac caaaaacggc agaagcttcc 1621 gggtgggaat tgatgctagc tgggcttgcc acgatttttg gtgtcattag cattgttttc 1681 ttttggcggc aacatcgcat ggccgtctaa ttcctgacaa taatcaaaaa tacggcccgc 1741 tacgcatctt ttgcgcaacg ggccgttatg gtgttgtgcg ccaacaccat ttcatagtgt 1801 cacgcaagga tttgatgaaa ttgtgatgtc attgagatga ttttggctgt tttgacaact 1861 acctcgccag catttacccg caaaataatc ggctagtaag gttccgtctt gttatttagt 1921 ttaattgagc cgcaagttta caaaataaag gtcagcggtc tagataggta ctgtataatg 1981 aacttgaaaa cgacgaaaaa gcagcgcaaa ggcaaattgg ttagaaaacg gcgtattagc 2041 ggacatgaat gagatgatgg ggtgttcatc atggggttca gaggtttaac atgcggattt 2101 ctgatctgaa gcgcttgttt aaaaaaggag tgggcatttt gacagaacca ttatttttaa 2161 aaccatactt taatcctaag atatggggcg gccgcaaact cgaaactgag tacggctttg 2221 acattccaga cggtaaaatc ggtgaggctt ggatcatctc aggtcatccg catggtccgg 2281 cgaccattga aaacggtcca ttgaagggta aaacccttcg cgaggcgtgg catgaagatc 2341 ccgcttattt tggtccacaa catagtcagg agttcccatt gctcgtgaag attctcgatg 2401 ctgaagctag tttaagtgtt caggttcacc ctgacgatgc ttatgctgcg gcccacgaag 2461 caaaaggcga actcggtaag actgaatgct ggtacatcct gaaggcagat ccaggggctt 2521 atttgatcta tgggcatcac gccaaaacgc atgaacagct caaagaaatg atcgaagccg 2581 gcgattggga gcacttgttg cggaaatatc cagtcaagac tggcgacttt gtgtatgttc 2641 caagcggcac gatacatgct ttaaacaaag gcatcgtggc attggaaacc caacaaagtt 2701 ccgatacgac ttatcggctt tacgactatg atcggaaaga tgatcaaggt cacaagcgtg 2761 agttgcatat taagcaaagc cttgatgtga cgaccgttcc tttccatgac ccgaagctcg 2821 atatcaccaa agaacaagtg acaggcgggc aaatcaccac gtttgttaaa ccgccgctgt 2881 caccgcattt tgccgtttat cgccttgatg ttgatggcac catgacgttc acccaacaag 2941 caccttatac caatgtaaca gtgcttgacg gtgacgggac cttttctgca gacgggaagg 3001 attacccgat caaaacaggc gatagtttct tgattcccaa ccagatcaag acttgggcat 3061 tcactggcaa attggcaatc gtcacttcta ctccaggggt agacgcataa ggttgcggat 3121 catcaggtgc ccaacatatt cataaagaca aaagttatcc ctcgacaggt ttttagacca 3181 tcgagggatt tttttgttac tggtgtgatt aaatggctgt taaacaaaaa attagaacgt 3241 aatgagaacg ctttcctatc gaaataatta ttttcctgat ttggtctaat tatggaaatg 3301 tctgttaccg ctgaattcat gatttcaatt tcttttttta acgaaaattg gtacatgttg 3361 tcattttgag aacctgtaag tcgttaaaaa taaaaataag tcgcatgtat tgacttcacg 3421 aactgggaac gcatacacta caagtggttg gtaccacgtg gaggtgagat gatggaaaag 3481 gttccgttga cgaaacagct ggtagatcag ctggaaacgt tcattaagga gaaacttcag 3541 cccaacgata agctgccgtc cgagcgggat ctcagcgagt tgtatcaggt cagtcgcaac 3601 acagttcgtg ctgccttgaa cgaattgttc ttgcgcggtt tcatttatcg aagcactggt 3661 aaaggtacat ttgttgcaga acgatttgat gagcgaaccg atgtcagtgg gtcctacagt 3721 ttcactaaac agatgttggc aatgaaccgt aaaccagtga ctagaatcca gcgtttagaa 3781 aaaattgcgg cacccgccaa aattgccaag cgcatgcgca tcgaaccagg cacgcccgtt 3841 tacgtccttg atcgattacg aatcgctgac acgttaccga tgatgattga gcgcagttac 3901 ttacctgcta acactgtgcc aggtcttaat caggaagcac tatcaaaaac gccattatac 3961 gattggctgg aaggtcatta tggcattcac attgtttcag tcgatgaagc gttttttgct 4021 ggattagtca cgccggaaga tgctgaacaa cttcgagttg caccagcgtc agcctgtttg 4081 aacattcggc gaacaacttt tgccgatacc ggcgagattg ttgaatacac cttgagtgtg 4141 gctcgcgcgg atcaatttgt ttatcacgta catcacgtta atcactagat aataaagcat 4201 aaaccagtcc ggcttagggg ctggcaagca ggtttccaat aggaggatga agaacattgt 4261 tcgaaaaaac aactgaagaa ctaaccaagt taggtgcaca aattacaact gctgaaatca 4321 aacagcagcc agaactatgg caagaagcat ttgataacta taccaacgcc aaggaaaaga 4381 tcacggcctt tttggatcag gtgacggccg cagctaatgg caaaaccgtt cgcgttattt 4441 tcactggtgc cggtagttca caatatgttg gtgacacggt gacgccatac ctacgcgcgc 4501 atggtgatcg cagcaagttc gaatttgaat caattgggtc aactgatatt gtgtccgcac 4561 cttacgattt tctggaagca gacacgccga ccattctggt ttcctttgct cgtagcggca 4621 actcaccgga aagtttgaaa actgttgaac tggctgaaca agttgttcac aacctccatc 4681 aaatcagcat tacctgcgca ccggaaggcc aattggccaa gaagtctgaa acggaagaca 4741 agactttgtt gctgttacag ccagctggtt caaatgataa aggctttgcc atgacaggca 4801 gtttctcttg catgaccttg agtgccttgc tggtatttga taccgccagt gacacggata 4861 agagcaagtg ggtgaaagcc attgcggcaa tgggacaaga agttgtggcg cgtgaagccg 4921 aagttcaaaa agtgcttgac cttgattttg atcgtatcgt ctatcttggc tctggcagtc 4981 ttggcggctt gacccgcgaa actcgcctga aggtgcttga actaacggct ggcaagtatg 5041 ccaccatgtt tgacacttcc atgggcttcc gtcatggtcc aaagagtttc ctagatgaca 5101 agactttgtt gttcgacctc gttagcaaca atgcctacac ccgtcaatat gatatcgatg 5161 tccttgagga agttaagggt gacggtattg ctaaatccgt gatggcagtg ggcaccaagc 5221 aagatcagaa cttctcaggc gatgccttct attttgaaaa tggccttaaa gatcttccag 5281 aagcttatca ggctctccca gatgtcatgt tcgcccagac tgtagcattg cttgcttcga 5341 tcaaagttgg caacttgcct gacacaccat caccaaccgg caccgttaac cgagttgtta 5401 aaggcgtgac cttgcacgat tacgctggta aggaatagag cgtgaactgg caccaagcag 5461 catcaacgag caagtttaga ggaggcaaaa gagttcatga gttactacat tcacgctgca 5521 aagttttttc tcaagaatgt gactgaaatt ggtggttatc tagaagtcac cgatgatggc 5581 aagtttggcg agtatctgcc ggaaacagct aagccagatg gcgatattgt tgaacaatcc 5641 ggcaaattga ttgctccagg tttagtcgat acccatattc acgggttgtt agggcatgac 5701 gtaatggaca acgattggga tggcattgag acaatgagca aaaacttgct caaagctggt 5761 gtcaccagtt ggctgccaac cacattgacc ggttcatttg atcaactcaa tgaggtttgc 5821 aaaacgattg ccgcacatgc tggcgatgaa agcggtgcga agattcaagg catttatttt 5881 gaaggcccat actttacgac caagcataaa ggggcacaga atccgaagta cttcaaaaat 5941 ccaagtgagc aagaatacga tgcttggcag acatccgctg atggcttgtt gaagaaaatt 6001 gcgattgccc cagaacgtga gggtgctgct gacttcactc ggtatgccac cgcagacggc 6061 acggtggtgg ctttgggcca tagtgatgcc acctttgagc aggctaaagc ttgtatcgaa 6121 gccggtgcaa ctgtcttcac acacactttc aatggtatga gcccgttgag ccatcgcgaa 6181 cctggtatgg tcggagcagc gatgtatctg caaggggttg atgatgagct tatctgtgat 6241 ggtcatcatg tccgtcctga agtggccacc accttgattc gactgaaagg cgctgaacat 6301 atcgcattga ttaccgattg catgcgtgcg gggatgatgc ctgatggcga ttacgtcctt 6361 ggcgaattcc cagtttatgt caaagacggg atggcgcgga tgaaagatgg cgacagtctg 6421 gcgggttcag ttcttgaatt gaaagatgcg ctgaccaatc tccttgcttg gaacgccgca 6481 acaccagaag caattattcg aatggcttct caaacaccgg ctgcttcttg caatatcgac 6541 gatcaatgcg gcagtatttt acctggccga gccgctgatt atctagtttt ggacgctgat 6601 ctcaagctcg aagccaccta tcttgatggc aaattaggct atcaagctga agcatgaaag 6661 ctttcctgac acaaaacctt gtgaaataag gtgtcagtgt gtgacactta aatatggcag 6721 cataagctgt tatgaatgaa aggaggccag cgctgcatga cgaccttttc gatcgatcat 6781 gagtttatgt tggacggtca gccgttcaag attctgtctg gcgcgattca ttacttccga 6841 gtccatccca gtgattggta tcacagcctt tataacttga aggcactggg atttaacacg 6901 gtagaaacct atgtcccttg gaatctgcac gagtacaacg aaggtgactt tgatttcagc 6961 gggattctcg acattgaacg ctttctaaac accgcaaagg atcttggctt atatgccatc 7021 gtgcgaccat ccccttacat ctgcgcagaa tgggagttcg gcgggtttcc agcatggctg 7081 ttaacgaaaa agatgcgctt gcgaaccgac gatcctgcct atctgcaagc gattgaccga 7141 tactacacag ctttaatgcc tcatcttgtg ggtcatcaag tcacgcatgg tggcaatgtc 7201 atcatgatgc aagtggaaaa tgagtacggt tcatatggcg aagacaagga ctatcttgcc 7261 gccgtggccg aacttatgaa aaagcatggc gttgatgtcc cgctttttac ctcggatggt 7321 ccctggccag caaccttgaa cgctggcagc atggccgacg ctggtatttt gactaccggc 7381 aattttggct cacgtgccga catgaatttc gatcggttgg cggccttcaa tcaagctcat 7441 ggacatgatt ggccgttgat gtgtatggaa ttctgggacg gctggttcaa ccgctggggc 7501 gaaccgatca ttcgccgcga cccagaagaa actgccgagg acttgcgagc ggtgatccaa 7561 cgcggcagtg tcaatctcta catgttccac gggggaacta attttggctt tatgaacggt 7621 acctcagctc gcaaagatca tgatctaccg caagtgactt cctacgatta cgatgcacca 7681 ttgaatgagc aaggcaatcc aacgccaaag tactttacca ttcagaaaat gatccacgaa 7741 gtcttgccat cgcaggccca gaccacaccg ctggtcaagc cagcgatgag gcaagcggac 7801 aatccgttga cggcgaaagt gtcattattc tcagtacttg atcagctagc tcagccagta 7861 gccgcacctt atccgcagac acaggagttt cttggccaat acaccggtta tacgttgtat 7921 cgaacaaacc cgctgatcag tggtaccgac aaaggcacgc cggctaagtt gcgcgtgatt 7981 gatgcccgtg atcgggttca agccttcttt gatggcaagt cgctagcaac acagtatcaa 8041 gaggccatcg gcgatgacat cctgctccca gaagttgaag gccgccatca actagatctg 8101 ttggtcgaaa acatgagccg cgtcaactat ggctcaaaaa tcgaagcgat tacccagttt 8161 aagggaattc gcaccggtgt catggtagac ctccatttca ttaaggacta cctgcaatac 8221 ccacttgact tgaacaaggc gccgcaactt gatttcaccg gcgattggca agcagggaca 8281 cccgcttttt atcaatatgg gtttgatgtg gtgaaaccac aagatacgta tcttgattgt 8341 cgtggatttg gtaaaggagt gatgctggtc aatggtgtta acatcggcag attctggggg 8401 aaggggccaa cgttgtcact atatgtgcca gccgggttgc ttcacaccgg gcacaatgag 8461 gtcattgttt ttgaaaccga aggccaatat gccgaggcga taaacttggt tgaccaccca 8521 atatttaagg aactgaacac agaggaggaa taaaaatggc tattattgca gcacgtattg 8581 atggtcgctt ggtccatggc caagttgcca atctctggac tactaagctc ggcgcaacgc 8641 ggattatggt cgtggacgag gccgcatcaa agagtgatat cgacaagagc ggattacgga 8701 tggcaacccc agatggtatt cgcctgtctg ttctcgatgt tgccacagca gcaaaccaca 8761 ttttgaatca caaatatgat tcacaaaagg ttttcattgt ggcaaagcgg ccggagacat 8821 tcctcgcatt ggtgaatgca ggtgttccga tcaccgactt gaacgttggt aatatgtctc 8881 aaactgatga aacccgttca atcacgaagt ccatcaacgt ggtggacgct gacgttgaca 8941 catttaaaga acttgatgca aaaggggttc atctcacagc gcaaatggtt ccaggcgaca 9001 cagcagcgga cttcatgtcc ctgctcaaaa aaggataggg ggagtcaaac atgattgcat 9061 ggtggcaaat agcactttta acattatatg caggctatca aattcttgat gaactgcaaa 9121 tttattcagc catgagttca ccagttttcg ccggcttaat ttccggcttg gttatgggcg 9181 atatgaaagc cggtctgttg atcggtgcat ccacacagtt gatggttctt ggggtcggca 9241 cattcggtgg tgcatctaaa attgatgcca actctggtac cattctcgca acggcattct 9301 ccgttagcgc cggcatgaag ccagaagcag cggttgctgc gattgcggtt cctgttgcaa 9361 gtatcatgat tcaaactgat attcttgctc gattctcgaa cactttcttt gctcatcgga 9421 ttgacaagat ggttgatcaa aataactaca aaggtatcga acgttacttc ctcgctggcg 9481 cgcttccgtg ggcactgtca cggatgattc cagttggctt ggctttagca tttggcggcg 9541 gcttcgttac cgaccttgcc catgttttga ataccagctg gaaatggtta ggcgatggct 9601 tggcaacagc tggtgccgtc ttgcctgctg tcgggtttgc cattctgttg cattacctgc 9661 cacttaagaa acacttccca tacctcatta tgggctttgt tttcaccgtg ctgttcacga 9721 cactgtttgg cagcgttcag accttgggta ccgcagttgc tggggtagct aaagggttca 9781 ctgcttcctt caacggtttg tccatgcttg ctatggcatt gattgcgttt gcctttgctg 9841 caatcagcta tcaacaaaca acaaagctgc acgatatcgc agttggcaat ggtaatacag 9901 atgcagcatc aggcacagcc gcaaacaaga ctaatgaaca gggggaaatc accgatgatg 9961 aactctaata aacctacgta caaattgacc gataaagatt tccggcagat taaccggcgc 10021 agcctgttcg gcttccagtt gggttggaac tacgaacgga tgcaaggttc gggttatctt 10081 tacaccattt tgccgcaatt gcgcaaaatc tacggtgatg gcacaccaga gctcaagaaa 10141 gtcatgaaaa cccacaccca gttcttcaac acgtcaaact tcttcaatac catcatcact 10201 gggattgatt tggcggtcga agaaaaagaa gggattgctg gcgaagaaac tgttgccggg 10261 attaaaaccg gtttgatggg gtccttcgct gctatcggtg attccatctt tgctgccttg 10321 attccagcta ttttcggggc gattgctgca caaatggcaa cgcaaggcaa cccagttggg 10381 gtcttcatct ggatcgctgc tcaaattgcc gtcatggtat ttcgttggaa gcaattgcga 10441 attgcttata acgaaggggt ttcgttggtc acaacaatgg cgcatcgatt ggctgctttg 10501 actgacgctg ctaccttgat gggtgtcttc atggttggtg ccttggtcgc aaccatgatc 10561 aatgttaaga ttggttttgc gccgaagatc ggcagtgtac cattggattt ccagaaatac 10621 tttgacttga tcattccaaa actgcttcca gccttgattg ttggcgcggt ttactggttg 10681 cttggtcgta agcatatgac ctcaacccgg gcgattttca tcgtattgat cgtttcggtg 10741 ctgctgtcgg cactcggtgt cattgctaag ggttaatttg gtatcagaaa cgttttaaag 10801 gagtctttcg atatgagtaa attagttttg attagtcacg ggcaactgtg tgaggagctt 10861 aagaagagcg ctgaaatgat catgggtccc caagacgata ttgccaccgt tgcactgttg 10921 ccaaatgaag gtccggaaga ttttcaggcc aagtttaaag ctgctgtaga ggggcaagac 10981 gatgttgtcg tccttgccga ccttaaaggc ggaacgccga ataacgtcgc agcccgtgtc 11041 ttggctgaag gcggcaagtt cgacctgtat tctggcatga acctgccgat ggtcatcagt 11101 ttcctgaatg cccagatgtt ggacatgaag ccagatctga tcggcgatgg taaggctggc 11161 attgcttata tcaatgacat tgtgaagcat taa //