LOCUS NZ_GG692727 9529 bp DNA linear CON 20-DEC-2019 DEFINITION Roseburia intestinalis L1-82 Scfld14, whole genome shotgun sequence. ACCESSION NZ_GG692727 VERSION NZ_GG692727.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN00008856 Assembly: GCF_000156535.1 KEYWORDS WGS; RefSeq. SOURCE Roseburia intestinalis L1-82 ORGANISM Roseburia intestinalis L1-82 Bacteria; Firmicutes; Clostridia; Clostridiales; Lachnospiraceae; Roseburia. REFERENCE 1 (bases 1 to 9529) AUTHORS Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (07-AUG-2009) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 2 (bases 1 to 9529) AUTHORS Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (03-JUN-2009) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 3 (bases 1 to 9529) AUTHORS Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (03-JUN-2009) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA REFERENCE 4 (bases 1 to 9529) AUTHORS Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (16-OCT-2008) Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 12/17/2019 04:46:02 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Method :: Best-placed reference protein Annotation Software revision :: 4.10 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; Genes (total) :: 4,283 CDSs (total) :: 4,204 Genes (coding) :: 3,739 CDSs (with protein) :: 3,739 Genes (RNA) :: 79 rRNAs :: 4, 2, 9 (5S, 16S, 23S) complete rRNAs :: 4, 1, 1 (5S, 16S, 23S) partial rRNAs :: 1, 8 (16S, 23S) tRNAs :: 60 ncRNAs :: 4 Pseudo Genes (total) :: 467 CDSs (without protein) :: 465 Pseudo Genes (ambiguous residues) :: 39 of 467 Pseudo Genes (frameshifted) :: 133 of 467 Pseudo Genes (incomplete) :: 320 of 467 Pseudo Genes (internal stop) :: 22 of 467 Pseudo Genes (multiple problems) :: 46 of 467 CRISPR Arrays :: 3 ##Genome-Annotation-Data-END## REFSEQ INFORMATION: The reference sequence was derived from GG692727. Roseburia intestinalis L1-82 (GenBank Accession Number for 16S rDNA gene: AJ312385 is a member of the division Firmicutes in the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from the laboratory of Prof. Harry Flint, Rowett Research Institute, Aberdeen, Scotland, UK (H.Flint@rowett.ac.uk). We have performed one round of automated sequence improvement (pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. This work was supported by the National Human Genome Research Institute and the NIH Roadmap Human Microbiome Project [http://nihroadmap.nih.gov/hmp/]. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Genome Coverage: 15.8X Sequencing Technology: 454. Roseburia intestinalis L1-82 (GenBank Accession Number for 16S rDNA gene: AJ312385) is a member of the division Firmicutes in the domain bacteria and has been isolated from human feces. The sequenced strain was obtained from the laboratory of Prof. Harry Flint, Rowett Research Institute, Aberdeen, Scotland, UK (H.Flint@rowett.ac.uk). We have performed one round of automated sequence improvement (pre-finishing), along with manual improvement that includes breaking apart any mis-assembly, and making manual joins where possible. Manual edits also are made where the consensus appears to be incorrect. All low quality data on the ends of contigs is removed. Contigs are ordered and oriented where possible. This work was supported by the National Human Genome Research Institute and the NIH Roadmap Human Microbiome Project [http://nihroadmap.nih.gov/hmp/]. Coding sequences were predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. tRNA genes were determined using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and Rfam v8.0. Gene names are generated at the contig level and may not necessarily reflect any known order or orientation between contigs. This is a reference genome for the Human Microbiome Project. This project is co-owned with the Human Microbiome Project DACC. Genome Coverage: 15.8X Sequencing Technology: 454 Annotation was added to the contigs in August 2009. Product names were updated in June 2013. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline (PGAP) set; GeneMarkS-2+ repeat_region FEATURES Location/Qualifiers source 1..9529 /organism="Roseburia intestinalis L1-82" /mol_type="genomic DNA" /submitter_seqid="Scfld14" /strain="L1-82" /isolation_source="biological product [ENVO:02000043]" /host="Homo sapiens" /type_material="type strain of Roseburia intestinalis" /db_xref="taxon:536231" gene complement(1..3927) /locus_tag="ROSINTL182_RS11930" /old_locus_tag="ROSINTL182_07683" /db_xref="GeneID:43319281" CDS complement(1..3927) /locus_tag="ROSINTL182_RS11930" /old_locus_tag="ROSINTL182_07683" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015522404.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="CBM27|GH26|CBM23" /protein_id="WP_044999186.1" /db_xref="GeneID:43319281" /translation="MRKRFMGKRLAAALLAAAVAFSSGNFAGGYEYVYAAEASVFYTGT NTVTKDKLDLATEDWDNPPMITYDESIADCVTTKDFTMKADITLDDEAYASLGTGESYL KVQGIVKLGSEWTWTDSQDIPYLQQSSFSEDTHKTTITIKFEDKDADDLKGVYFRLIGQ GFSGTCTFSNVTLSGVAEAQPELPAKDPSVIDDFESAETGSSAGWEQEGGWQYDNAVGI SVAEFQGSKMLKADLDYTGCEGFTWSEAKIKKSFAEGLDVSAYNVLSYEMIYPEAFDGS FKAKIFAKNGADDVEIINKEAKIETSDLGDGYKKAVVTVKFSPNTAKITDLMIGTVGVS TAFLGSVYLDNLTLSQYNAAGDYTEITETAGEAVLADTSSMPEEVTLSDVNASGSAKAL YAYLKGLDAADQVLFGHQNDTSKHVSTRDGVYSDTKDVTGSISGLVGIDSLALTGVELG IDNVDDAVQKSIEISKAAAAEGAIITLSTHMPNMSNEKIIATPDAARKYDFSQCDFSEA KDLSNNCSAEVLPGGKYNAQFTTYLDIIADYANGLGDIPVLFRPFHENTGGWFWWGAAT TDKETYRALFQYTQDYLASKGVHNFIYVYSPNGPLTSEEEYMDRYPGDDCVDIVAFDYY DDYNTYPAEYSGEFVDNLKKTCQVVKNIADAKGKVAAISETGVRVMKADGSDNEGILVK GNPIAGHNWYKQVNQVAVDTGMPYFLLWANFGDTNFYVPYKYDDTHGQELINEFIEFYN EKSSVFANGTNFYGNADQKEVRNVNQGNAAGYFANVFSKMAITSAFTLKANVRNAQSVS FVLTNPDTNTEKTITAEKTEESGAYEGVLSADILSALGTTDTGKISLVADGKTLVTVSY ISFGKEKETLAKNVIENFELYYDDNDYLNGTFTENSAANCSSSFVLDKDHKAEGVYGGA FTYQLKTGGPEVWTGRMKGLSTNDYSEYNAVSMWVKPDGKGQKLVVQLVSGGEDFEVFL TDFVKTTEAKYVTIPFNKLKGKQNGTFDPKNVTKFAIWCNSISEDGNGVDIQSKIVFDD IRFVNVDESKLNVTEGGYVLTDQSLVSGGQKPGDTEKPGDTEKPGDTEKPGDTEKPGNT EKPGDTEKPGNTDKTTVAKVSGFKQSRATETSIRLTWKKVKKADGYQVYRYNKSTKKYQ KVATVKTNTYTDKKLKTATVYQYKVRAYRKSGKKTVTGKYSNVIKAATSPQKVTAVKAK RVKSKVKLTWKKVKGADGYEIYRATSKKGKYQKIKTLKKGTIVSYTDGKAKKGKTYYYK VRAVKNAGKTSVKGTASNAVRCGK" gene complement(4120..6924) /locus_tag="ROSINTL182_RS11935" /old_locus_tag="ROSINTL182_07684" /db_xref="GeneID:43319282" CDS complement(4120..6924) /locus_tag="ROSINTL182_RS11935" /old_locus_tag="ROSINTL182_07684" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015559372.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH3" /protein_id="WP_006857766.1" /db_xref="GeneID:43319282" /translation="MGNSGIGVPLPELAAYCREAAAEGAVLLKNEGHMLPIKKDETVSI FGRSQIEYYRSGTGSGGAVNVPYVKNILDGIKENNAFPVNEDLVETYKEWLKEHPFDNG GGGWAAEPWHQEEMEITDEIARRAAEKSEKAIFLIGRTAGEDKDYEDTEGSYLLTKREK ENLRVVTKYFNEVAVLLNVSNIIDMSWTKDAAYQDHIKAILYIWQGGMEGANAVADLLS GRVTPSGKLTDTIAEKLSDYPAADHFGSKTENIYAEDIYVGYRYFETFAPEKVMYEFGF GLSYTEFSMEMVKAESTGNGKDAKIALSIRVKNTGAAAGKEAAQVYVSAPQGQLGKPAR VLCGFAKTKLLAPGEEEVLELTIPVSRFASYDDSGVTGHKSCYVLEEGLYKIYVGNSVR CTEKANVDGKGGYEVSSCIVTEELEEALAPTKEFLRLKTGRQKEDGVFARAYEKAPQQM VDLAERIKSRLPKELPQTGNKGITLQAVAENIKNGSSVEEELDAFVAQFTNEELAVIVR GEGMSSPKVTPGTASAFGGVSDSLHGYGIPIACASDGPSGIRMESGLKATQLPIGTLLA CSFNIPMMEELYQMEGRELVGNEIDTLLGPGINIHRYPLNGRNFEYFSEDPLVTGQFAA AMTRGIRSAGSSATVKHFAANNQETERHNVNSVVSERALREIYLKGFEIAVKEGNANSI MTSYNPVNGHWTASNYDLNTTILRGEWGYQGIVMTDWWAKMNDVVNGGEADRRYTSFMV RAQNDLYMVVNNNGAAINAAGDDTVEALEAGKLTVGELQRCAKNICRFLLGTPVMKRPL KDFDPLLTVTAKEAVNTDEKQVCALQSGNRIEAGKFENTVLYVEKESILNVNAHFCFPV EGLAQGAANLYLNGELMATVQTGGTGERFLTQRMCRVKFMPGYYELKAEYVTSELKLDW IELE" gene complement(7112..9529) /locus_tag="ROSINTL182_RS11940" /old_locus_tag="ROSINTL182_07685" /db_xref="GeneID:43319283" CDS complement(7112..9529) /locus_tag="ROSINTL182_RS11940" /old_locus_tag="ROSINTL182_07685" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006857767.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH3" /protein_id="WP_006857767.1" /db_xref="GeneID:43319283" /translation="MEKWQRSLYQPNLPLGADGTKVTASKAHITLSKEAAKEGMVLLKN NESLLPFQAGTKLALFGKGSFDYVKGGGGSGDVTVAYTTNLYEGFKKLPEKVEVYEALS DYYRKEVEKQYEAGAEPGMTVEPAFPEETAKKARAYTDTAVICISRFSGEGWDRKSSYD KEMDESVQTDPLLEKAERIFPDGDFYLTKEESAMVEQVQQLFPKVAVVMNVGGMVDTDW FAENEKIQAVLMAWQGGMEGGSAAAELLCGVGSPSGKLSDTFAKKLEDYPSSETFHESV KYVDYKEDIYVGYRYFETIPGADKAVNYPFGYGLSYTTFERALVSAEEKQGVIRVRVNV TNTGKYPGKEVMQLYAQAPQGVLGKAKRVLAAFEKTRLLAQGETQLLTLEAPVAQLASY DDLGKIQKSAYVLEKGKYQFYLGTSVRETEQVFCFTMPEDTVTEQLTAKLVPTLLAERM LSDGSFEKLPQSEPNDPDYSAIKRVPREESDGFSPAVRALPGHQIWSQPYKKDAHIFME VAEGKITLDEFVTQMTDEELAHILGGQPNTGVANTFGFGNMPEYGIPNIMTADGPAGLR IEKKCGVVTTAWPCATLLACTWNPDMLYQVGAAGAAEVKENNIAVWLTPAVNIHRSPLC GRNFEYYSEDPYLTGKMASAMVKGIQSKHIAATVKHFACNNKETNRKESDSRVSERAAR EIYLKAFEIIVKEADPWCIMSSYNIVNGHRTSENRELLEDILRGEWNYQGMVTTDWWTS GEHYKEVKAGNDIKMACGFPESLLRAKEAGVLTREEMEICAKRILGLILKID" ORIGIN 1 ttattttcca cagcgcactg catttgaagc agttcctttc accgatgttt ttccggcatt 61 ttttacggct cttactttat agtaatatgt ttttcctttc ttcgctttac cgtctgtgta 121 agaaacaata gtgccctttt tcagcgtctt tatcttttga tattttcctt ttttggatgt 181 tgcacggtag atctcgtatc catctgcacc ttttaccttc ttccaggtta attttacctt 241 ggacttcaca cgttttgcct taaccgcagt caccttctgc gggctggtcg cagccttgat 301 cacattggag tactttccgg ttaccgtctt tttaccgctt ttcctgtatg cacgcacttt 361 atactgataa accgttgctg ttttgagctt tttatccgta taggtattag tttttaccgt 421 tgcaaccttc tgatattttt tcgttgattt gttataacgg tatacctgat aaccatctgc 481 tttctttact tttttccatg ttaagcggat ggaagtctcc gttgccctgg actgtttgaa 541 gccggaaacc tttgcaactg tcgttttatc tgtattaccc ggtttctctg tatctcccgg 601 tttctctgta tttcccggtt tttctgtatc tcccggtttc tctgtatctc ccggtttctc 661 tgtatctccc ggtttctccg tatctcccgg tttctgtcct ccggacacga gtgactgatc 721 tgtcagtaca tatccacctt ctgtcacatt taactttgat tcatccacat ttacaaagcg 781 gatatcatca aaaacaattt tcgactggat atcaacccca tttccgtctt ctgaaatgct 841 gttacaccag attgcaaact tcgtaacatt ctttggatca aaagttccat tctgtttacc 901 ctttaatttg ttaaatggga tcgtaacata ttttgcctct gtcgtcttta caaagtcagt 961 cagaaatact tcgaaatcct ctccgccgga aacaagctga actaccagtt tctgtccttt 1021 tccatccggt tttacccaca tgctgaccgc attgtattcg ctgtaatcgt ttgtgcttaa 1081 acctttcata cgtccggtcc atacttccgg tccgcccgtc ttaagctgat aagtaaatgc 1141 acctccgtaa acaccttctg ctttatgatc cttatctaat acaaaggaag aactgcagtt 1201 tgctgctgaa ttttctgtaa aagttccatt caggtagtca ttgtcatcat agtataattc 1261 aaaattttca atgacattct ttgcaagtgt ttccttttct tttccaaaac tgatatagga 1321 aacagtcaca agtgttttgc catctgccac aaggctgatc ttaccggtat ccgttgttcc 1381 aagtgcactc agaatatccg cagacagcac accttcgtaa gcaccggatt cttccgtttt 1441 ctctgccgtg atcgtttttt ctgtatttgt atccgggttt gtcagcacaa aacttacact 1501 ctgtgcattt ctcacatttg cttttaaagt aaatgcactt gtgattgcca tcttagagaa 1561 aacattggca aaataacctg ctgcatttcc ctgatttaca tttcttactt ctttctggtc 1621 tgcatttccg taaaagtttg ttccattcgc aaaaacagaa gatttttcat tgtagaattc 1681 aataaactcg ttgatcagtt cctgtccgtg ggtatcatca tatttatatg ggacataaaa 1741 gttggtgtct ccaaaatttg cccacagtaa gaagtacggc attccggtgt caactgccac 1801 ctgattaacc tgtttgtacc agttgtgtcc tgcgatcgga tttcctttta ctaaaattcc 1861 ttcattatcc gatccatcgg ccttcatcac ccttacaccg gtttcggaaa ttgcagctac 1921 ttttcctttt gcgtctgcaa tattctttac cacctgacag gttttcttta aattgtccac 1981 aaactcgccg gaatattctg ccggataagt attgtaatca tcatagtaat caaatgcaac 2041 gatatccaca cagtcatcgc ccggatagcg gtccatatac tcttcctctg aagttaacgg 2101 accatttggg gaatatacat aaataaagtt gtgtactccc tttgatgcca gataatcctg 2161 tgtgtactgg aacagcgcac ggtatgtctc tttatcggtt gtcgcagcac cccaccagaa 2221 ccatccgcct gtattttcat ggaatggacg gaataaaaca ggaatatcgc caagtccgtt 2281 tgcgtaatct gcaatgatat caagataggt tgtaaactgc gcattgtatt ttccacccgg 2341 aagtacttct gcggagcagt tattgctcag gtcttttgcc tccgaaaaat cacactgtga 2401 aaaatcatat tttctcgccg catccggtgt tgcaatgatt ttttcattgc tcatattcgg 2461 catatgggtg gataatgtaa tgattgcacc ctctgcagct gctgccttgc tgatctcgat 2521 agatttctga actgcatcat ctacgttatc aattccaagt tccacgcctg tcagtgccag 2581 cgaatcgatt ccgaccagac cactaatact tccggtcacg tcctttgtat ccgaatagac 2641 accgtctctg gttgaaacat gcttgcttgt gtcattctga tgtccgaata atacctggtc 2701 agcagcatcc agtcctttta aatatgcata taacgctttt gctgaacctg atgcattcac 2761 atccgaaagt gttacttctt ccggcatact ggacgtatct gcaagcacag cctctcctgc 2821 cgtctctgtg atctccgtat aatcgcccgc tgcattatac tgggataaag tcaggttatc 2881 cagatatacg cttcccaaaa aggcagtgct cacaccaact gttccaatca tcagatccgt 2941 gatctttgca gtgtttggtg agaatttgac agtgaccact gctttcttat atccgtcacc 3001 gagatctgac gtttcgatct ttgcttcttt gttgatgatc tccacatcat ctgctccgtt 3061 ttttgcaaaa atctttgcct taaaagaacc gtcaaaagct tccggataga tcatttcata 3121 gcttaaaaca ttataagccg atacatcgag tccttctgca aacgactttt taatctttgc 3181 ctcactccat gtaaaacctt cacaaccggt ataatcgaga tctgccttta acattttgct 3241 tccctgaaat tcagctacac tgattcctac cgcattgtcg tactgccagc cgccttcctg 3301 ctcccagcct gcactgcttc ctgtctctgc agattcaaaa tcatcgatca cactcggatc 3361 ttttgcaggc agttccggct gcgcttctgc cacaccgctt aatgtaacat tggaaaatgt 3421 acatgttcct gaaaagccct gaccaatcag tcggaaatat acacctttta aatcatctgc 3481 atccttatcc tcaaacttga tcgtaatcgt tgttttatgt gtatcttctg aaaaactgct 3541 ctgctgtaaa tacgggatat cctgactgtc tgtccatgtc cactcacttc ccagcttcac 3601 gataccctga acctttaagt aactttcccc ggtacccaat gatgcgtacg cctcatcatc 3661 cagtgtaata tctgccttca ttgtgaaatc tttcgttgtc acacaatctg caatactttc 3721 atcataagtg atcattggcg gattatccca atcctctgtt gcaagatcca gtttgtcttt 3781 tgttactgtg tttgtcccgg tataaaacac actcgcttct gccgcataga catactcgta 3841 tccccctgca aaattgccgc ttgaaaacgc aactgcagcc gctaaaagag cggctgcaag 3901 tcgttttccc ataaaacgtt ttctcataaa cttacctcca atacatttat tagctttatg 3961 cactttgtct tttaattata tgttacttta aaaaacattg tcaattaaga ttatattagt 4021 ttgtaaattt tcagagtttt tacttaattt ttgctttttt aagttgtttt atgagattaa 4081 aattgtatac ttttacttta taaattcaga taataaattt tactctaact caatccagtc 4141 caacttcagt tccgatgtca catactctgc tttcagttca taataccccg gcataaattt 4201 cacacgacac atccgctgtg tcaggaaacg ctctccggtt ccacctgtct ggacggttgc 4261 catcagttct ccattcagat acagattggc agctccctgt gctaatcctt ccaccgggaa 4321 acagaaatgt gcattaacgt ttaaaatgct ttctttttcc acataaagga ctgtgttttc 4381 aaactttcct gcctcgatcc ggtttccgct ctgcagtgca cagacctgct tttcgtctgt 4441 atttactgct tcttttgcag taactgtaag cagtggatca aaatctttta acggacgttt 4501 catcacaggt gttccaagca ggaaacggca gatgtttttc gcacagcgct gtaactctcc 4561 aacagtgagt tttcccgcct ccaacgcttc tacggtatca tcgcccgctg cgttgatggc 4621 agcaccatta ttgttgacta ccatgtataa gtcattctgt gcacgaacca tgaatgaggt 4681 gtatctgcgg tctgcctccc cgccgtttac aacgtcattc atctttgccc accagtctgt 4741 cataacaatt ccctgatatc cccactcgcc acgcaggatc gtcgtattta agtcataatt 4801 ggatgccgtc cagtgtccgt tgaccgggtt gtaagaggtc atgatcgaat ttgcatttcc 4861 ctctttcacg gcaatctcaa agcctttcag atagatttca cggagtgccc gctcagatac 4921 aacggagttt acattatgtc tttccgtctc ctgattattc gctgcaaaat gtttcacggt 4981 cgcagaagaa ccggcacttc ggattcccct tgtcattgcc gccgcaaact gtcctgtcac 5041 aagcggatct tcactgaaat actcaaagtt acgtccattt aatggatagc ggtggatatt 5101 gattcccggt ccaagcaggg tgtcgatctc gtttccaact aattccctgc cttccatctg 5161 atataattct tccatcattg gaatattaaa ggaacatgca aggagtgttc cgatcgggag 5221 ctgtgttgcc tttaatccgc tctccatacg gatgccggac gggccatcgg atgcgcaggc 5281 aatcgggatg ccataaccat gcaggctgtc gctgacaccg ccaaatgcgg atgctgtacc 5341 cggtgttacc ttcgggctgc tcattccctc gccgcggacg atcacggcta actcctcgtt 5401 ggtaaactgt gctacaaatg cgtccaattc ttcttccaca ctgctgccat ttttgatatt 5461 ctctgcaacc gcctgtaagg taattccttt atttccggtc tgtggcagtt cttttggcag 5521 acggcttttg atccgctctg ccagatcaac catctgctgt ggtgcttttt catatgcacg 5581 ggcaaacacc ccgtcctcct tttgtctgcc tgtcttaaga cgcaaaaatt cttttgtcgg 5641 cgcaagcgcc tcctccaact cctctgtgac gatacaggaa gaaacctcat aaccgccttt 5701 tccgtccaca ttggcttttt ctgtgcatct gacgctgttt ccaacataga tcttatacag 5761 accttcctct aagacatagc aggacttatg accagtcaca ccgctgtcgt cataggaagc 5821 aaaccggctt accggaatgg taagttcaag tacttcctcc tcgcccggag caagcagttt 5881 tgtctttgca aatccgcaca atacccttgc cggttttcca agctgtccct gtggggcgga 5941 cacataaacc tgtgcagcct cttttccggc tgctgccccg gtatttttca cacggatact 6001 gagtgcgatc tttgcatctt ttccatttcc tgtactctcc gcttttacca tctccatgga 6061 aaattccgta taagataaac cgaaaccaaa ctcatacatt actttttccg gtgcgaatgt 6121 ctcaaaataa cggtagccga catagatatc ttccgcataa atattttctg tcttacttcc 6181 aaaatggtct gctgccgggt aatcagaaag tttctccgcg atcgtatccg tcagttttcc 6241 ggatggtgtc actctgcccg ataacagatc tgcaacggcg ttcgcgcctt ccataccacc 6301 ctgccagatg taaagaatcg ctttgatgtg atcctgatat gccgcatctt tcgtccagct 6361 catatcaatg atgttagaca catttaaaag cactgccact tcgttaaaat attttgttac 6421 aacacgaaga ttttctttct cccgttttgt cagaagataa ctgccttccg tgtcctcgta 6481 atctttatcc tcaccggcag ttcttccgat cagaaaaatt gctttttcag acttttctgc 6541 tgcgcggcgt gcgatctcgt cggtgatctc catctcttcc tgatgccacg gctctgccgc 6601 ccatccaccg cctccattgt caaacggatg ctcctttaac cattctttat aggtttctac 6661 cagatcctca tttacaggaa aagcattatt ttctttgatt ccgtctaaaa tatttttgac 6721 atacggcaca ttgaccgctc cacctgaacc ggttccgctc cggtaatatt cgatctggct 6781 tcttccgaaa atagaaactg tctcatcctt tttgatcgga agcatatgcc cctcattctt 6841 taaaagcacg gctccctctg ccgctgcctc tctgcagtat gctgccagtt ccggcagcgg 6901 aacaccgatt ccactgttac ccatgataat cctccatttt aatctccatt cttgcgctgt 6961 ctttcaatga aaacaacgtt ttcatttgca tctcaagttt gtgcgtaagc acaaatcttg 7021 cgtgtgaaaa atcttatttt tcacactata tatctgttgt tatcttttta gcctgtcatt 7081 atttttgtct atgattgttt ttcgtctgat ctcagtcgat tttcagtatt aatcctaaaa 7141 tacgctttgc gcagatctcc atctcttctc ttgtcagcac tcctgcttct tttgcacgga 7201 gcagactctc cggaaatcca catgccatct tgatgtcatt tcccgctttt acttctttat 7261 aatgctcacc gcttgtccac cagtcggtcg tgaccattcc ctgataattc cactcacctc 7321 ttaagatgtc ctctaaaagt tcacggtttt cagaagttct gtgtccgttt acgatattgt 7381 aagatgacat aatgcaccac ggatctgcct cttttacgat aatctcaaac gcctttaaat 7441 aaatctccct tgctgcccgc tcggacactc tggaatcgct ttccttacgg tttgtctcct 7501 tattgttgca tgcaaaatgt ttgaccgtcg cggcaatatg ttttgactgg attcccttta 7561 ccattgcaga tgccattttc ccggtcagat acgggtcttc ggaataatac tcgaagtttc 7621 ttccgcaaag cggacttctg tggatattga ccgcaggcgt cagccagacg gcaatgttat 7681 tttccttcac ctcagcggca cctgctgcac caacctgata taacatatcc gggttccagg 7741 tacatgctaa aagtgtcgca cacggccacg ccgtcgtaac tacgccgcat tttttctcaa 7801 tgcggagtcc ggcaggtccg tccgccgtca taatattcgg gattccatac tccggcatat 7861 ttccaaatcc aaatgtattt gccacaccgg tattcggctg tccgcctaaa atatgcgcaa 7921 gttcctcatc cgtcatctgt gtcacaaatt catccagcgt gatctttcct tctgcaactt 7981 ccataaaaat atgtgcgtct ttcttatacg gctgtgacca gatctgatgt cccggcagtg 8041 cgcggacagc cggtgaaaat ccatcgcttt cctccctcgg aacacgtttg attgcggagt 8101 aatccggatc attcggttcg gactgcggca gtttttcaaa acttccatcg gagagcatgc 8161 gctccgcaag taaagtcgga accagttttg cggtaagctg ctctgttacg gtatcctctg 8221 gcatcgtaaa acaaaatacc tgctctgttt cccgcacgga cgtgccaaga taaaactgat 8281 acttaccttt ttccagcaca taggcagatt tctgaatttt tcccagatca tcataggatg 8341 caagctgtgc caccggtgcc tctaacgtca gaagctgcgt ttctccctgt gctaacagtc 8401 gtgttttttc aaatgctgca aggactctct ttgcctttcc aagtacgccc tgcggtgcct 8461 gcgcatataa ctgcatgact tcttttcccg gatattttcc ggtgttggtc acattgacac 8521 gcacgcggat cacaccctgt ttttcttccg cagatactaa cgcacgctcg aaagtcgtgt 8581 aggaaagacc gtagccaaac ggataattga cggctttatc agcccccggt attgtctcaa 8641 aatagcggta gccgacgtag atatcttctt tataatcgac atattttacc gattcatgga 8701 atgtctccga tgacggatag tcttccagtt tctttgcaaa tgtgtcggaa agttttccgg 8761 atggactgcc gacgccgcac agaagctctg ctgctgcgct tccgccttcc atacccccct 8821 gccatgccat cagcaccgcc tgaatttttt cattttctgc aaaccagtcc gtatcgacca 8881 tgccgccgac attcataacg actgccacct tcgggaacaa ctgctgcacc tgttccacca 8941 tcgcgctctc ttccttcgtc aggtaaaagt ctccgtccgg aaaaatgcgc tctgcctttt 9001 ccagcagcgg atctgtctgt accgactcat ccatctcctt atcataagaa ctctttctgt 9061 cccagccttc cccggaaaaa cggctgatgc agatcactgc cgtgtccgtg tacgctctcg 9121 ctttttttgc agtttcttcc ggaaacgcag gctctactgt cattcccggt tctgctcctg 9181 cctcgtactg tttttctacc tctttacggt aataatcaga gagtgcctca tacacttcta 9241 ctttttccgg cagcttcttg aatccctcat acaaatttgt cgtataagca acggttacat 9301 ctccgcttcc gccaccaccc ttgacatagt caaaactgcc ttttccaaac agggcgagtt 9361 tggtgcctgc ctgaaatggg agaaggcttt cattgttctt tagaagaacc atgccttctt 9421 tggcggcttc ttttgacagg gtgatgtgtg cttttgaggc ggtgactttg gtgccgtcag 9481 ctccgagagg gaggttgggt tgataaagtg atctttgcca tttttccat //