LOCUS NC_013132 10061 bp DNA linear CON 26-AUG-2019 DEFINITION Chitinophaga pinensis DSM 2588, complete genome. ACCESSION NC_013132 VERSION NC_013132.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN00001914 Assembly: GCF_000024005.1 KEYWORDS RefSeq. SOURCE Chitinophaga pinensis DSM 2588 ORGANISM Chitinophaga pinensis DSM 2588 Bacteria; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga. REFERENCE 1 (bases 1 to 10061) AUTHORS Glavina Del Rio,T., Abt,B., Spring,S., Lapidus,A., Nolan,M., Tice,H., Copeland,A., Cheng,J.F., Chen,F., Bruce,D., Goodwin,L., Pitluck,S., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Chain,P., Saunders,E., Detter,J.C., Brettin,T., Rohde,M., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lucas,S. TITLE Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034) JOURNAL Stand Genomic Sci 2 (1), 87-95 (2010) PUBMED 21304681 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 10061) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Chitinophaga pinensis DSM 2588 JOURNAL Unpublished REFERENCE 3 (bases 1 to 10061) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (12-AUG-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 08/26/2019 00:28:39 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Method :: Best-placed reference protein Annotation Software revision :: 4.9 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; Genes (total) :: 7,171 CDSs (total) :: 7,077 Genes (coding) :: 7,009 CDSs (with protein) :: 7,009 Genes (RNA) :: 94 rRNAs :: 6, 6, 6 (5S, 16S, 23S) complete rRNAs :: 6, 6, 6 (5S, 16S, 23S) tRNAs :: 73 ncRNAs :: 3 Pseudo Genes (total) :: 68 CDSs (without protein) :: 68 Pseudo Genes (ambiguous residues) :: 0 of 68 Pseudo Genes (frameshifted) :: 19 of 68 Pseudo Genes (incomplete) :: 47 of 68 Pseudo Genes (internal stop) :: 11 of 68 Pseudo Genes (multiple problems) :: 8 of 68 ##Genome-Annotation-Data-END## ##Metadata-START## Organism Display Name :: Chitinophaga pinensis UQM 2034, DSM 2588 Culture Collection ID :: DSM 2588, ATCC 43595, LMG 13176, UQM 2034 GOLD Stamp ID :: Gi02244 Funding Program :: DOE-GEBA 2007 Gene Calling Method :: Prodigal Isolation Site :: Pine litter in Australia Isolation Country :: Australia Oxygen Requirement :: Aerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 23 - 24C pH :: 7 Gram Staining :: gram- Biotic Relationship :: Free living Diseases :: None Phenotypes :: Biomass degrader, Chitin degradation Energy Source :: Chemoorganotroph ##Metadata-END## REFSEQ INFORMATION: The reference sequence was derived from CP001699. URL -- http://www.jgi.doe.gov JGI Project ID: 4082984 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation Pipeline (PGAP) set; GeneMarkS-2+ repeat_region COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..10061 /organism="Chitinophaga pinensis DSM 2588" /mol_type="genomic DNA" /strain="DSM 2588" /type_material="type strain of Chitinophaga pinensis" /db_xref="taxon:485918" /note="type strain of Chitinophaga pinensis" gene complement(1..1431) /locus_tag="CPIN_RS30430" /old_locus_tag="Cpin_6154" CDS complement(1..1431) /locus_tag="CPIN_RS30430" /old_locus_tag="Cpin_6154" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008645802.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="RagB/SusD family nutrient uptake outer membrane protein" /protein_id="WP_012793727.1" /translation="MKSGIYHFAYILVIAILTSCNKYLDIVPKGQLVVETTQDFYNMVS LPNRGYPINNFQYLVDDQWMKESNVIGIIKNMDIINFTADTTENRVNRLSASSLYNQAY KYINRWNMIITLVDGSEGDENLKKLAKAEARVQRAFDHFVVLNTFGRPYVPETAANDGG ICIMDKYDLEAKPSKSSVAEVYAFIEKDLDESIPYLQVTPVDVYHPSLAFAWALKAKVH LFKREWDKCIAAAQQSLSYNNKLLDLVALKAAGGPTLNPIAAGNNPEVLSYTYMTGRNE LNLGYTFIISPELRALFGPTDTRFTMFFNTTNRTYLDIGSNTAYWQVKFTDFFTVTVGM KTPEVLLMLAECYARKGSLTEAMTLINDLRAKRITDAQEAHLETPATVKETMDILISER RKELLFGFHRFWDLRRLNLEPEYAKTVVHKFPLVNTTVPQVEYKLPPNSYMYIIPFAQD VLKQNPKLTLNTNEKLPW" gene complement(1450..5178) /locus_tag="CPIN_RS30435" /old_locus_tag="Cpin_6155" CDS complement(1450..5178) /locus_tag="CPIN_RS30435" /old_locus_tag="Cpin_6155" /inference="COORDINATES: protein motif:HMM:TIGR04056.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="gnl|TC-DB|Q45780|1.B.14.6.1" /protein_id="WP_083781171.1" /translation="MFFYALCNRKGRAMSCARRQSFRLSPQTVKTVKLNVLFMFAFLMQ VSASGWSQVINYSGNNVPLTTIFSIVKQQTGYVVLYNPDLVKKTTPVTVRASNMPLTAF LDKVLAERSLGYSLEDKTIFITKRTTPARPAVNEAAVEKAAVILPVVSGFIKDDKGQFL PGVSVKVKGSLFGTSSSESGYFKLTGIPADAVLAISSVGFESLNIPLQMCCNRVMVKNV DATMGDDGSLVLTITMKLAVKSLEEIAVTNTGYQELPKERSTGAYGTVDNKALNAQINT DLGAAVEGKVAGLSLYKGEPMVRGLSTFSGSVGTYPLIVLDGLLTENTLDMINPYDVES ITVLKDAAAASIYGARAANGVIVINTKKGKRGGVSLSVNADYFVTERPDLSKMHYATSG QMVDYERDIYAGELRRYANAQELFNYYGSVGGNTSRYFSPLYNLYRKESIGEISTHERD AAIEQLKSYDYYQQFHDNVWQNQVRKRFNISLGTGSDKSNTYFSINYDGNNQLIRNNTD ENLNIYLKSSFSPLPWLTATFGANGAYSKVIASENLYTDYKTQPRYSRIVDENGNRVYA DYVSFRDGFSSSDYVNGTVIDQLKGNTQFKSFSFNLLDELDNSRSKSQQLRLRTFADIG AKLFAGLKYSLKFQYETSRNEQESFAGKDDYRMRYLYNAMTSYNATTNIYTHEIPFSGR LYQMNQQADNFTFRQQLDFDRSFNVNGRTHDFNAIGGFEMRQSRTPVSVSDLRYGYDPV TLTSQQVDWYRLGESGINSYLFGTTTLGYQPGKNKTEVRHRFASYYGNIGYTFDRRYNV TASARIDQTDLFGTDPKYRNRPLWSVGAGWNATNEAFLKNISWLDFLKVRVTYGVNGNV DQTSSPYITARLRSDNLFTELQYTDILALPNPKLRWEKTATLNFGTDFTIWNNLLRGSI DYYRKYSSDLIATSTLDPTVGTSSLSINNGAMSNRGIELNLSSEWLNKKDFGAVSSFVI AFNRNRIEKVSNAATTGYSYISSPSNYYYPNTPFNSVYAYRYGGTINGYPYILDENGQP NVIFDANGNPTDVKQVNGPAAIIRVGTLIPKFNGSFRQSFRYKGVELGAMFAFYGGHQM RRDRMDFSGTTQQNRELANRYKDEVPNGNPRLELDYPDVLRSYATTLSSYWRYSDIQVV SATSVKLRNLYLSYTLPQYLCKNIFAKQVKFTAQANNLWMWSKAGDDLDPETASLNSGT RNLPTSRSFLFGAAVSF" gene complement(5311..6486) /locus_tag="CPIN_RS30440" CDS complement(5311..6486) /locus_tag="CPIN_RS30440" /inference="COORDINATES: protein motif:HMM:PF16344.3" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="STP|FecR" /protein_id="WP_012793729.1" /translation="MAEDTREERIKYLINQYYAGTATVAESSELRSFLLQGDDRELVVA QLEELAMTAPAATHISEEQLDTVFDSIVSSRTRQMPVRRYIYGAAAAVLALVVAGSIYF FTREHQSVSPQQVNVAEIQPGHDGAVLTLANGKQVVLDNQADGVIGNTDENISKKGGAL IVDGDAQSHNNTALNTLTTPRGRQFALALPDGSKVWLNAASAIKFPAAFAQEERTVYLS GEGYFEIAKKTDAKGKRIPFRVVVNQKSTIEVLGTHFNINAYEDEESIRTTLLEGAVKV SASTNTVTLVPGQQAAIIADHIAVDKHADLEEATSWKNGLFVFNGRADLREVMRQLSRW YDVEIVFKGEVPDIAFTGEMQRNLNLQQVTKGLAAMGVNFKIEGRKIIIYP" gene complement(6579..7145) /locus_tag="CPIN_RS30445" /old_locus_tag="Cpin_6157" CDS complement(6579..7145) /locus_tag="CPIN_RS30445" /old_locus_tag="Cpin_6157" /inference="COORDINATES: protein motif:HMM:TIGR02985.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DBD-Pfam|GerE" /protein_id="WP_012793730.1" /translation="MVPPLDNEIFLFRQIGEGNEIAFRKVYYHYYRQLYGFVLKAVKSD PVTEEIVQETFARLWENRAALGEKGYSAPWLFRVAANLSFDYMRKAAHEAKYFNLLSAS PGEPSANNVADYIEGVQSNHLLHEAIDSLPPQRRTIFRLSRLKGLSHQEISQHLNISEN TVKNQLVTALKAVRQFMNDATRLFF" gene 7244..8368 /locus_tag="CPIN_RS30450" /old_locus_tag="Cpin_6158" CDS 7244..8368 /locus_tag="CPIN_RS30450" /old_locus_tag="Cpin_6158" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008154329.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="GH130" /protein_id="WP_012793731.1" /translation="MQKSGLLFTMLLSGALYSYGQQSINKYPDWAIGPFVRPTGVNPII SPDSTTHFYDPMQKKLLDWESNDTFNPAAAVKDNKVYVMYRAEDKSGVGIGARTSRIGL AESSNGITMRRTGKPVLFPDMDDQQEFEWTGGCEDPRVAVTEDGTYLMLYTQWNKKVPR LGAATSKDLVHWKKHGPIFQDAYNGKFHDIASKSASVLTTLKNGELRIAKYKGKYWMYW GENHVYAATSENLVDWTPLVDANGALKELASPRKGYFDSHLTECGPPAILTSKGIVLLY NGKNRSGADGDINYTANSYCAGQMLFSAADPSKLISRLNKPFMVPAESFEKSGQYPAGT VFIEGLVYYKGKYFLYYGCADSRVAVAVYTPSAN" gene complement(8538..10061) /locus_tag="CPIN_RS37200" /old_locus_tag="Cpin_6159" CDS complement(8538..10061) /locus_tag="CPIN_RS37200" /old_locus_tag="Cpin_6159" /inference="COORDINATES: protein motif:HMM:PF00652.20" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="CBM13|GH43_26" /protein_id="WP_012793732.1" /translation="MKKLHLLCLSALMLLLSGPGCKKAEDKQDRLNGAAAFKGLTALAA SPIFTNALPSIDNGSYYDPWVIRVGAYYYYCGSDGGRLWIAKSATLQNLLQQQKSYIFT PPGGTLYSSNLWAPELHYRSGRWYVYFAADNGDNANHRMHVLEGGTDANDPLNGGYAYK AKLTPTTDRWAIDGSPFDFNGQQYFVWSGWEGTTNVSQYIYIARMSNPYTISGERVEIS RPDYGWEQVGSPTVNEGPTPLISGSTVNIIYSASGSWTNDYCLGRLTCTNGNLMAKSSW TKPSSAAFSRFGNVFGPGHASFVKSPDGIQSWIVYHAANYDGAGWDRRVMAQQFAWDGD IPFFGYPIEKGIPIPSPSTGPEYAMPVANGVYRIKSKVTSQCIDVPNASNTAGLQIQQW TDNNTNAQKWTFTSLGNGYYKITNVASGLNLDNAGGSTSAGNILIQWTDNGNIAQHWRV QDMGNGYFKLINRRSLLAIDVPNSNQTPGTKLQQWYENQYDAELWQIIP" ORIGIN 1 ttaccaggga agcttttcat ttgtattaag agttagttta ggattctgct tcaatacatc 61 ctgtgcaaac ggaatgatgt acatatatga attagggggc agtttatatt ctacctgtgg 121 cacagtcgta ttgaccagcg ggaatttatg cactaccgtt tttgcatatt ccggttcgag 181 attcagtctg cgcaggtccc agaagcgatg aaagccgaac aggagttctt tccttctttc 241 gctgatgagg atatccatcg tttcctttac agtagctggc gtttccaggt gagcttcctg 301 tgcatcggtg atacgtttgg cacgcaggtc attaatcagt gtcatcgctt cggtaagact 361 tcctttacgg gcataacatt cagccagcat taacagcact tccggcgttt tcatacccac 421 agtcactgta aagaagtctg taaatttcac ctgccagtaa gcagtatttg agccaatatc 481 caggtatgta cggttggtgg tgttaaagaa catagtaaac ctcgtgtcag taggaccgaa 541 cagtgctctt aactcaggac taataataaa tgtgtatcct aagtttaact cattacgacc 601 cgtcatgtat gtataactaa gtacttccgg attattgccg gctgcgattg gattcaaggt 661 ggggccgcct gctgctttca atgctacaag gtcaagcagc ttgttattgt acgacaatga 721 ctgctgcgcc gcagcgatac atttatccca ttctcttttg aagagatgta ccttcgcttt 781 cagcgcccat gcaaatgcga gagatggatg gtatacatcc acaggcgtta cctgcagata 841 tggaatagat tcgtcaagat ctttttctat gaaagcatac acttccgcta ctgatgattt 901 cgatggtttc gcttccagat catacttatc catgatgcag ataccgccgt cattggctgc 961 cgtttccggc acatatggac ggccaaatgt attcaggaca acaaaatgat caaatgcacg 1021 ctgaactctc gcttcggcct ttgccagctt cttgagattt tcatctccct cgctgccatc 1081 aacaagcgta atgatcatat tccatctgtt aatgtactta tatgcctgat tatacaggga 1141 ggaagcgctc agcctgttga cgcgattttc agtagtatca gcagtgaagt taataatatc 1201 catgtttttg atgataccga tcacattgga ctctttcatc cactgatcgt ctaccaggta 1261 ctggaagtta ttaatcggat atcctctgtt ggggaggctt accatattat aaaagtcctg 1321 cgtagtttcc actaccagtt gtcccttcgg aacgatgtca agatatttat tgcacgatgt 1381 aagaatggca ataacgagaa tatatgcgaa gtgataaata cctgatttca tacgctaatg 1441 ttttaataat tagaaactaa ccgctgcacc gaacaggaat gaacgcgatg taggcagatt 1501 tctggtccca ctgttaaggc ttgctgtttc cgggtcaaga tcgtccccgg ctttactcca 1561 catccacagg ttattcgcct gtgcagtgaa ctttacctgt ttagcgaata tgtttttgca 1621 caggtattgg ggcaaggtat aggataagta aagattacgt agcttgacag aagtagcgct 1681 caccacctga atatctgaat aacgccagta agaagacagt gtagtagcat aacttctgag 1741 gacatcaggg tagtccagtt ccagtctcgg attaccattc ggaacttcat ctttatagcg 1801 attagccagt tcgcggttct gctgggtagt acccgaaaag tccatacgat ctcttctcat 1861 ctgatggcca ccatagaaag caaacatagc acccagttct acgcctttat agcggaaact 1921 ctgacggaat gagccgttga acttagggat cagtgtacct actctgatta tcgcagcagg 1981 accatttacc tgtttaacgt ctgtaggatt cccatttgca tcgaaaatca cattaggctg 2041 gccgttctca tccagtatat acgggtatcc attgatagtt ccgccatatc tgtaagcgta 2101 tacggagtta aacggtgtat tgggataata gtagtttgac ggagacgata tatatgaata 2161 tccggttgtc gcggcattgc ttaccttttc tatcctgttc ctgttaaagg cgatcacgaa 2221 tgacgacacg gccccgaaat cttttttatt cagccattct gaagaaagat tgagttcgat 2281 accacgatta gacatcgcac cgttgttgat gctgagggag ctggtaccta ccgtagggtc 2341 aagcgtgctt gtagcaatca ggtctgagct gtattttctg tagtaatcaa tactacctct 2401 cagcaggtta ttccatatag taaaatctgt accgaagttg agcgtcgccg ttttttccca 2461 gcgtaacttc gggttaggca atgcaaggat gtctgtatac tgtaattcag tgaacaggtt 2521 atcacttctc agtctggcgg tgatatatgg agatgaggtc tgatcgacgt taccattcac 2581 accataagtc acgcgcactt tcaggaagtc aagccagctg atatttttca ggaatgcttc 2641 atttgttgcg ttccatcctg caccgactga ccacagcggg cggttcctgt atttcggatc 2701 tgtaccaaac aggtcggtct gatctatcct tgcactggca gtgacgttat aacgtcggtc 2761 aaaggtataa ccgatattac catagtaaga tgcaaatctg tggcgtacct ctgttttatt 2821 ctttcccggt tgataaccaa gtgtagttgt accaaacagg taactattga tccccgattc 2881 accaaggcgg taccaatcca cctgttggga agtcaatgta acaggatcat atccgtaccg 2941 caggtcgctt acagataccg gtgtgcggga ctgtctcatt tcaaaaccgc caatggcatt 3001 gaaatcatgc gttctgccat tgacattaaa tgacctgtcg aaatcaagct gctggcggaa 3061 ggtaaagttg tccgcctgct gattcatctg gtaaagacgt ccgctaaagg gaatttcgtg 3121 tgtataaatg ttagtcgttg cattatagct tgtcattgcg ttgtagagat acctcatgcg 3181 gtaatcatcc tttcctgcaa aactctcctg ttcattccgc gacgtttcgt actggaactt 3241 caggctgtat ttcaatcccg caaacaactt agcgcctata tctgcaaatg tacgcaaacg 3301 cagttgctgt gatttactcc tgctattatc cagttcatcg agcagattga aactgaaaga 3361 tttaaattgt gtattgccct ttagttgatc aatgacagta ccatttacgt aatcagagct 3421 actaaaaccg tctctgaatg atacatagtc agcataaacg cgattcccat tttcatctac 3481 gatgcgacta tagcgaggtt gtgtcttata gtcagtatag agattctccg atgctattac 3541 tttcgaataa gcaccgtttg caccgaatgt tgccgtcagc cagggaagcg gactaaagct 3601 tgatttcaga taaatgttca ggttctcgtc ggtgttattg cgtatgagct ggttgttacc 3661 atcgtagtta atggaaaaat acgtattgct cttgtcactg cctgtaccaa gtgaaatatt 3721 aaaacgtttc cgcacctggt tctgccatac attatcatga aactgctgat aatagtcgta 3781 gcttttcaat tgctcgatgg cagcgtctct ttcatgtgta ctgatctcgc caatgctttc 3841 ctttctgtac agattgtaca atggagaaaa gtatctactc gtgtttccgc caacgctacc 3901 atagtagtta aataattcct gcgcatttgc ataccggcgt aattcacctg catagatatc 3961 cctttcataa tcaaccatct gcccggaagt agcgtaatgc atcttactaa ggtcaggacg 4021 ctctgttaca aagtaatctg catttactga gagactgacc cctcctcttt tacctttctt 4081 tgtattaatc acaatgacgc catttgcggc acgcgcacca taaatggacg ctgcagcagc 4141 atctttgagt acggtaatag actcgacatc ataagggttg atcatatcca gggtattttc 4201 agtaagcagt ccgtcgagta ctatcagcgg atacgtacct acacttcctg aaaatgtact 4261 caatcccctg accattggct cacctttgta aagactcagg cccgctacct taccttcaac 4321 agcggcaccc aggtctgtat tgatctgggc attgagcgct ttattgtcaa cagttccata 4381 agcaccggta gaacgttctt ttggcaattc ctgataaccg gtattcgtaa cagcgatctc 4441 ctcgagtgat ttcacagcga gtttcatggt aatagtcagt accagactgc catcatcacc 4501 catggtagcg tcgacattct ttaccatgac cctgttacag cacatctgca atgggatgtt 4561 aagagattcg aaccctacac tggagatagc cagcacagca tcagcaggta tacccgttaa 4621 cttgaaataa ccggattcac tactgctggt accgaaaagt gagcctttca cttttacaga 4681 gacacccggc aggaactgtc ctttgtcatc ttttatgaaa ccacttacta ctggcagaat 4741 gactgccgct ttctcaacag cagcttcatt gactgccgga cgcgcaggcg tggtacgctt 4801 tgtaataaag atggtcttat cttccaggga atagcccaat gacctttctg ccaggacttt 4861 gtccaggaag gcggtcagcg gcatattgct ggcacgcacc gttacaggtg tagtcttctt 4921 taccagatcc ggattgtaga gtaccacgta gccggtttgc tgtttaacaa tgctgaagat 4981 tgtcgtaagc gggacattgt tcccggaata gttgatgacc tgtgaccagc cgctggcact 5041 aacttgcatc agaaatgcaa acatgaatag aacattcagt ttcactgtct tcactgtttg 5101 tggtgataac cggaaagact gccgtctggc acaggacatg gcacgcccct tccggttaca 5161 taaagcgtaa aaaaacatac ttttgtaacg gtttctggtt gataatgaca aataagtggt 5221 atcggaccta cttgctgtta aattactcgg acctgagact ttgccgggag tgctggaaca 5281 cttccggttt tttcttttgg tactttgata ttaaggatag ataataattt ttcgtccctc 5341 tattttaaaa ttgaccccca tcgcagctag tcctttggtc acctgctgca agtttagatt 5401 tctttgcatt tcaccggtaa aagcaatgtc cggcacttct cctttaaaaa cgatttccac 5461 atcgtaccat cttgacaact ggcgcatgac ttcacgcagg tcggctctgc cattgaaaac 5521 aaacagtccg ttcttccagg aagtagcttc ttccaggtcg gcatgtttat caacggcgat 5581 gtgatctgcg ataatagcgg cctgctgacc ggggaccagt gttacagtgt ttgtgcttgc 5641 gctgaccttt acagcgcctt cgagcaaggt agtacggata ctttcttcat cttcataagc 5701 attaatatta aaatgtgttc caagcacttc tatcgtgctt ttttggttga ccacaaccct 5761 gaacggtatt ctctttcctt tggcatctgt ttttttagcg atctcgaaat agccttctcc 5821 tgatagataa acagtgcgtt cttcctgtgc aaaagcagca ggaaatttaa tagccgaagc 5881 ggcattcagc catactttac taccatcggg caatgccagt gcaaattgtc ttccccgagg 5941 cgtggtaagc gtattcagtg ctgtattgtt atgtgactgt gcgtctccat caacgatcag 6001 cgctccccct tttttgctga tattctcatc tgtatttccg ataacgccgt cggcctggtt 6061 atccagtacg acctgttttc cattggcaag tgtcagtaca gcgccatcat gccccggctg 6121 tatttctgct acatttacct gttgtggtga cacagactgg tgttcgcgcg taaagaaata 6181 aatgctacca gccactacca atgccagtac ggctgcggct gcgccataga tataacggcg 6241 aacaggcatt tgtctggtac gggaagatac gatcgagtca aatacagtat ccagttgttc 6301 ttcactgata tgcgtagctg caggcgctgt cattgccagc tcttccagtt gcgccactac 6361 aagctcccta tcatctccct gtaagagaaa tgatctcagt tcgctggact ctgcaacagt 6421 tgcagtacct gcatagtatt gattgataag atatttgatc cgctcttcgc gtgtatcttc 6481 tgccatattt tggttgtggt tgaaaagagg taaagaggct cctttgcaat taataacgtt 6541 tgagaaaaga cgagggacta tccagtttga aaaaaaactt aaaaaaagag gcgggtagca 6601 tcgttcatga actggcgaac agcctttaaa gcggtcacca gctggttttt aacggtattt 6661 tcagaaatat tgaggtgctg ggaaatttcc tggtgactga gcccttttaa tcttgataac 6721 cggaaaatgg tgcggcgctg gggaggtaaa ctgtcgatgg cctcgtgtaa aagatggttg 6781 ctttgtacgc cctctatata gtcggctaca ttattggcag aaggttcacc gggagaggct 6841 gacaacagat taaaatattt tgcttcatga gcggctttcc tcatataatc aaaggagagg 6901 ttggcagcaa cacgaaagag ccagggggca gaatatcctt tttctccaag agcagcgcgg 6961 ttttcccaca aacgggcaaa cgtctcctgc acaatttctt ctgtgacagg gtcggacttt 7021 acagccttga gcacgaaacc atataattgc ctgtaatagt ggtaataaac cttacggaat 7081 gcaatctcat ttccttcccc aatctgcctg aataaaaaaa tttcattatc taatggagga 7141 accatgaccg cataaaagta attgttttcg gcagaaaatt atagcactgg cttccgcagg 7201 aaatgttgta ttataccagt catattctac aaattctcgt tatatgcaaa aatcaggtct 7261 cttatttacg atgctcctat cgggcgccct ttacagctat gggcagcagt ccatcaataa 7321 atacccggac tgggcgatag ggccctttgt cagacccaca ggcgtcaacc cgatcatctc 7381 acccgattcc acgacccatt tttacgatcc tatgcaaaaa aagctactgg actgggaaag 7441 caatgacacc ttcaatccgg ccgctgccgt aaaggataac aaggtctatg tcatgtacag 7501 ggctgaagac aaatccggtg taggaatagg cgcccgtacc tcgcgtatcg gactggcgga 7561 aagttccaat ggcatcacca tgagacgtac cggcaaaccg gtactgttcc cggatatgga 7621 cgatcagcag gaatttgaat ggacaggcgg ttgtgaagat ccacgggtag cagtgacgga 7681 agacggcacg tatctgatgt tatatacaca atggaacaag aaagtgccac gccttggtgc 7741 ggctacctcc aaagacctgg tacactggaa aaagcatggt ccgatcttcc aggatgccta 7801 caatggtaag ttccatgata ttgcttccaa atcagcctcc gtattgacca cactcaaaaa 7861 cggtgagctg cgtattgcaa aatacaaagg taaatactgg atgtactggg gagaaaacca 7921 tgtatatgcg gctacctccg aaaaccttgt cgattggaca ccattggtgg acgcaaatgg 7981 tgcattaaag gaactggctt ctccacgaaa aggatatttt gatagtcacc tgacggaatg 8041 cggtcctccg gctatactga ccagcaaagg cattgtactc ctgtataatg gtaagaaccg 8101 ctcaggggca gatggagata ttaattatac cgccaattct tattgcgcag gacagatgtt 8161 gttcagtgca gcagatccat ccaaactgat cagcagactg aataaaccat ttatggtacc 8221 agcagagtcg tttgagaaaa gcggacagta tccggcaggg acggtgttta ttgaaggctt 8281 agtgtattat aagggaaagt atttcctgta ttatggttgt gcggattcaa gagtagcagt 8341 agcggtgtat acgccttcgg cgaattagga attaacaatt aaaaattaag aattgattga 8401 taataatgct gaaactgata aagaggatgt attaaaataa acgaccattc tcagaattgc 8461 tgaaatgacc acctgattcc atcaggtatt caaataaaaa gaggctgctc aagtgttggc 8521 agcctctttt tatttatcta aggtataatt tgccacaact ctgcatcgta ttggttctcg 8581 taccactgtt gcaatttcgt tccaggcgtc tgattactgt taggtacatc aattgccagc 8641 aggctcctgc ggttgatcag tttgaaatag ccgttgccca tatcctgtac ccgccagtgt 8701 tgagcgatat tgccattgtc tgtccactga attaggatat tccctgcact tgttgaacca 8761 cccgcgttat cgaggtttaa gccggaagcc acgttggtga tcttataata accgttcccc 8821 agactggtaa atgtccattt ctgtgcgttg gtgttgttgt ctgtccattg ttgtatctgg 8881 agcccagcgg tattacttgc gttaggcaca tcaatacact gggaggtgac tttagacttg 8941 atcctgtaca caccattggc aacaggcatt gcatattcag gaccggttga aggagaaggt 9001 atcggaatcc ctttttcaat cggatagccg aagaaaggaa tatctccatc ccaggcgaat 9061 tgctgcgcca tcactcttct atcccagcca gcgccgtcat agttagcagc atggtacacg 9121 atccatgact ggataccatc aggcgatttt acaaacgagg catgaccagg accaaatacg 9181 tttccgaatc gtgagaaggc ggcagaagag ggtttggtcc aggaggactt agccatcagg 9241 ttgccgtttg tgcaggtaag acgccccagg cagtaatcgt tggtccagct accgcttgca 9301 gagtagataa tattcacggt actgccactg atcaatggtg tgggaccttc gttgacagta 9361 ggcgaaccga cctgttccca gccataatca ggtcttgaga tctctaccct ttcgccagaa 9421 attgtgtaag gattgctcat tctggcgatg tagatgtact gggaaacatt ggtggtacct 9481 tcccaaccag accagacaaa gtattgttgt ccgttaaagt cgaaaggaga accgtctatc 9541 gcccagcgat cggtagtagg agtaagtttc gctttgtaag cataaccgcc gtttaaagga 9601 tcgttggcat cagtgccacc ttccaatacg tgcatacggt gattggcatt atcgccattg 9661 tccgcagcga aataaacata ccatcttccg ctacggtaat gcagctcggg cgcccataag 9721 ttagaggaat agagcgtgcc tcccggcggc gtaaagatgt agcttttctg ctgttgtaat 9781 aagttctgca aggtagcaga tttcgcaatc cacaggcggc ctccgtcaga gccgcaatag 9841 taataataag cgcccacacg gatgacccat ggatcataat agctaccatt gtcaatagag 9901 ggtaatgcgt tggtaaaaat gggagatgca gcaagagcag tgagaccttt gaaggcagca 9961 gcgccattca gccggtcctg tttatcttct gcttttttac aacccggacc tgataagagc 10021 agcattaacg cggaaaggca taacaaatga agttttttca t //