LOCUS AB063629 7232 bp DNA linear BCT 14-DEC-2007 DEFINITION Pseudoalteromonas piscicida chiD, cbp1, chiA genes for chitinase D, chitin-binding protein 1, chitinase A, complete cds. ACCESSION AB063629 VERSION AB063629.1 KEYWORDS . SOURCE Pseudoalteromonas piscicida ORGANISM Pseudoalteromonas piscicida Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas. REFERENCE 1 AUTHORS Tsujibo,H., Orikoshi,H., Tanno,H., Fujimoto,K., Miyamoto,K., Imada,C., Okami,Y. and Inamori,Y. TITLE Cloning, sequence, and expression of a chitinase gene from a marine bacterium, Altermonas sp. strain O-7 JOURNAL J. Bacteriol. 175 (1), 176-181 (1993) PUBMED 8416892 REFERENCE 2 AUTHORS Tsujibo,H., Orikoshi,H., Baba,N., Miyahara,M., Miyamoto,K., Yasuda,M. and Inamori,Y. TITLE Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7 JOURNAL Appl. Environ. Microbiol. 68 (1), 263-270 (2002) PUBMED 11772635 REFERENCE 3 (bases 1 to 7232) AUTHORS Tsujibo,H. TITLE Direct Submission JOURNAL Submitted (22-JUN-2001) Contact:Hiroshi Tsujibo Osaka University of Pharmaceutical Sciences, Department of Microbiology; 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan COMMENT On Jan 14, 2002 this sequence version replaced D13762.1. FEATURES Location/Qualifiers source 1..7232 /organism="Pseudoalteromonas piscicida" /mol_type="genomic DNA" /strain="O-7" /db_xref="taxon:43662" /note="originally submitted as 'Alteromonas sp. O-7'" gene 1..3150 /gene="chiD" CDS 1..3114 /gene="chiD" /note="ChiD" /codon_start=1 /transl_table=11 /product="CBM5|GH18" /protein_id="BAB79618.1" /translation="MKQHVKTLKLSQMASILGAIFCANSYAYNCDGLPEWSQSSAYNGG AQVQKSQQAFEAKWWTQADPVTHSGQWDDWKKLGDCDSVVTNTPPTVQSLLPADGSAFN ENDSVAISVNATDSDGQVTQVEFFVGGTSVGIDTTATGDNFSFNWSATAGTHAISVVAT DDKQAVSNTLITNIIVNTTGNQLPTANLSLGGGNNLVINQPVDIVLDGSDPDGSIAKLT LLINDQVAYESTTAPAQHTWTPTQLGSVTLKLVVEDNTGATAESILNANVVETPPVVNS CVPHGLYQTPNISPNYCDIYDENGREKMGTDHPRRVIGYFTSWRTGKNGQPSYLVDDIP WDKITHINYAFAHVDANNKVSIGNPTAPNNAATNMTWPGVAGAEMDPSLPYTGHFNLLN KFKKQHPDVKTLISVGGWAETGGYFDETGKRIASGGFYTMTTNADGSVNHAGIDAFVAS SVEFIRKYNFDGVDIDYEYPSSMNDSGHPDDFAISNPRRAALNASYQVLMKKLREALDQ AGQQDNTHYLLTIASPSSGYLLRGMETFQVTKYLDYVNIMSYDLHGAWNQHVGHNASLF DTGEDSELKQWNVYGTKEFEGIGYLNTDWAVKYFRGSVSAGRINIGIPYYTRGFRDVTG GTNGLWGQAALPNQADCPPGTGSGEKNKCGNGAVGIDNLWHDKNDVGLEVPAGSNPLWH VKNLQNGVLGSYLSDYGLDPVNNPEHQISGTYARHYDSVAVAPWLWNATKNVFLSIEDE ESMAAKVDYVINQELGGIMFWELAGDFDYDVSKNEYFMGSSLTSLAYNKFNQSGTPYSV KQGNKTFVKPASAVDVTFVAKDFPAGDANYPIAPTFAFTNNSAIDLSGAKISFDVPVAT SAIFKSNWNAQEKLGMAVDVNGSNAAGNNIGGFENEFHRFSITLVNEWGGTPKSFAPGE TINAQVMYYMPISGPVNFVVEKDGNRYAFSSEYSDLPIATPGTGPTDPVTSCKGTPISQ IVVYPNLPQGTHAAQGDLIVEGNAVYEAKWWSNKQPSVSGDYTKACNL" sig_peptide 1..81 regulatory 3137..3150 /regulatory_class="terminator" /gene="chiD" regulatory 3147..3150 /regulatory_class="ribosome_binding_site" /gene="chiD" /inference="non-experimental evidence, no additional details recorded" gene 3160..4609 /gene="cbp1" CDS 3160..4587 /gene="cbp1" /note="Cbp1" /codon_start=1 /transl_table=11 /product="CBM5|AA10" /protein_id="BAB79619.1" /translation="MNSLHRKYALGLFGATSALISSAVSAHGFMDFPKARQAICQAQGG YWWPDDGSNIPNLACRAAFLDSGHFQFVQEHEFSTNTADYNNLEAVKANIPNGTLCSAG DPNKSGMSVVSPHWQRTVVEPNAQNKLAISFRATTPHNPSFWQFFLSKPNYDGDTSPLT WNDLELINEFGNIDFVVDPEGKRFYKMEIDIPQGREGNAVLYTRWQRFDVAGEGFYNCS DITITSTGTPPEWVSSGYFLKQGQDAKAGDSVWFRTFDQSGNELISHNLNVTSTNASTW QAELALYLNSNYGNKVNIGVKQPDGSIAFDANALLANEVFLPHAAYTSNLSVVKGGDNT APTVNPISNQSIAENSQATVHAHAFDDQNDPLTFIWSIPAPLSFSGEGDTISISTPEVT ADQTFEAELNVTDGKASTTVSFSITVQDSTNNQYPAWNSTTTYVAGDRVTHQQKVYEAK WWTQGEEPGASDVWKAI" sig_peptide 3160..3237 regulatory 4593..4609 /regulatory_class="terminator" /gene="cbp1" regulatory 4627..4631 /regulatory_class="ribosome_binding_site" /inference="non-experimental evidence, no additional details recorded" gene 4640..7232 /gene="chiA" CDS 4640..7102 /gene="chiA" /note="ChiA" /codon_start=1 /transl_table=11 /product="CBM5|GH18" /protein_id="BAB79620.1" /translation="MKLNKITSYIGFALLSGGALAAPSTPTLDWQPQQYSFVEVNVDGL GSYKQLVKAKDVVDISIKWNAWSGSGGDNYKVYFDDLLVNQGSLPAGTKSGVVQFPYTK SGRHQLYLELCEGTVCARSAGKEIVIADTDGAHLAPLPMNVDPNNRNNGTIPGRVTGAY FVEWGIYGRNYDVTKIPAHNLSHILYGFIPICGPNESLKSIEIGNSWRALQTACADSQD YEVVIHDPWAAVQKSMPGVDAKDPIRGVYSQLMALKQRYPDLKILPSVGGWTLSDPFHG FTNKANRDTFVASVKQFLKTWKFYDGVDIDWEFPGGDGPNPDLGDPINDGPAYVALMQE LRAMLDELEAETGRQYELTSAIGAGYDKIEDVDYQAAQQYMDYIFAMTYDFYGAWNNET GHQTGIYCGSHLSTDECNGTGVDDNGVPRKGPAYTGDHAIQLLLQQGVQPSKLVMGVAM YGRGWEGVLDANAAIPGNPMTAPGNGPLTGSTSEGVWEPGIMDYKAIAANAVGQGGSGV NGYEVGYDEQAQAAYVWNRSNGKLITYDSPRSVIAKGQYANTHQLAGLFGWEIDADNGD ILNAMYDGLTAGEIPNRAPTIGVSGPINVTSGQVVNVDAQASDLDNDPLTYSWVAAPGL ALSANNTAAVAVTAPSVAQQTSYDLTVTVNDGALSTTKTIVVVVNPEGANAAPVVTPVS DISVNEGASATVNVSATDPEGAALSYSWSVPAELSVANGSSATITAANVTADTTVPVTV TVSDGVNAVDTTFNVTIKDGAEYPTWDRSTVYVGGDRVIHNSNVFEAKWWTQGEEPGTA DVWKAVTN" sig_peptide 4640..4702 regulatory 7212..7232 /regulatory_class="terminator" /gene="chiA" ORIGIN 1 atgaaacaac atgtaaagac ccttaaatta tcgcaaatgg cttcgatcct cggagccatt 61 ttctgcgcta actcatatgc ttataattgt gatggtttac ctgaatggtc acaaagttcg 121 gcatataatg gtggtgctca agtacaaaaa tcgcaacaag cttttgaagc aaagtggtgg 181 actcaggctg atccagttac ccattccgga caatgggatg attggaaaaa attgggcgac 241 tgtgacagcg ttgtcaccaa cacaccacct acagtacaaa gcttactccc agctgacggc 301 agcgctttta acgaaaatga cagcgttgcc atttctgtaa atgcaacaga tagcgatggc 361 caagtaacgc aggtagagtt ttttgtaggt ggcacgtctg ttggtattga taccacagcg 421 acaggtgata atttttcatt taactggtca gcaaccgctg gaacacacgc aatttcagta 481 gttgcaacag atgacaagca agctgtgtcc aacacattaa taactaatat tatcgttaat 541 acgacaggaa atcagttacc tacagcaaac ctttcgttag gcggaggtaa taatctagtt 601 attaatcagc ctgtagacat cgttcttgat ggttcagatc cagacggctc aatcgcaaaa 661 ctaacactgc ttattaacga ccaagtcgca tatgagagca caactgcacc ggcacagcac 721 acttggacac caacgcaact gggctccgta acattaaagc ttgttgttga agataatacc 781 ggtgcgacag cagagagtat actcaatgca aatgtggttg aaacaccgcc agtggtaaac 841 agctgtgttc cgcatggtct ttatcagacc cccaacatat cgcctaacta ctgtgatatc 901 tatgatgaaa atggccgtga aaaaatgggt actgatcacc ctagacgcgt catcggttac 961 tttaccagtt ggagaacggg taagaatggt caaccgagct acttagttga tgatattcca 1021 tgggataaga tcacccacat caattatgct ttcgcgcacg ttgatgccaa caacaaggtt 1081 tcaataggca atccaactgc tcctaacaat gcagcgacga atatgacttg gccgggggtt 1141 gctggtgcag agatggatcc atctcttcct tataccggtc attttaactt gctgaacaaa 1201 tttaagaaac agcatcctga tgttaaaacc ttgatctcag tcggcggttg ggcggaaact 1261 ggtggctact tcgacgaaac aggtaagcga attgccagcg gcggtttcta tacgatgacg 1321 accaatgcag atggcagcgt gaatcacgca ggtattgatg cgtttgtagc aagttcagtt 1381 gaatttatcc gtaaatacaa ctttgatggt gtcgatattg actatgaata cccatcttct 1441 atgaatgact ctggtcaccc cgatgatttt gcgatatcaa acccgcgtcg agcagcattg 1501 aatgcttctt atcaagtatt aatgaaaaaa ctgagagaag cattagacca agcaggtcaa 1561 caagacaata ctcactattt attaacgatc gcatcaccat cttctggtta ccttctacgt 1621 ggtatggaga cattccaagt cactaagtat ctagactacg tcaatattat gtcttatgac 1681 ttacacggag cgtggaacca gcacgttggc cataacgcat cgctattcga caccggcgaa 1741 gattccgagc ttaaacagtg gaatgtttac ggcaccaaag agtttgaggg aattggttac 1801 cttaataccg attgggcagt caaatatttc agaggctctg tctctgcagg acgcatcaac 1861 ataggtatcc cttattatac tcgcggcttt agagatgtaa caggtggtac aaatggtttg 1921 tgggggcaag ctgcattacc taaccaagcc gattgtcctc cgggaactgg cagcggtgaa 1981 aagaacaagt gcggtaatgg tgcagtgggt attgataacc tgtggcacga caagaacgat 2041 gttggcttag aagtaccggc aggctctaac ccgctttggc atgttaaaaa ccttcaaaat 2101 ggcgtgttag gaagttacct aagtgattat ggactagatc cagttaacaa cccagagcat 2161 cagattagcg gtacttatgc acgccactat gattccgtag cggttgctcc ttggctttgg 2221 aacgccacca agaatgtttt cttatccatt gaagatgaag agtcaatggc cgctaaagtc 2281 gactatgtga tcaaccaaga actaggcgga atcatgttct gggagcttgc tggtgacttc 2341 gattatgatg taagcaaaaa tgaatacttc atgggttcat cgctaacaag cttagcctac 2401 aacaagttta accaaagtgg cacgccatac tccgttaaac aaggaaataa gacctttgta 2461 aaaccagctt ctgcagttga tgtcacattc gttgctaaag atttcccagc tggagatgct 2521 aactatccaa ttgcaccgac ttttgccttt accaataact cagcgataga tttgagtggc 2581 gcaaaaattt cgtttgatgt tcctgttgca acatcggcaa ttttcaaatc taactggaat 2641 gcacaagaaa agcttggcat ggcagtggac gtaaatggat cgaatgctgc gggtaataat 2701 attggcggct tcgaaaacga attccaccga ttctcgatca cgcttgtgaa cgagtggggt 2761 ggtacgccta agtcttttgc tcccggcgaa actatcaatg ctcaagtcat gtactacatg 2821 cctatttctg gccccgttaa ctttgtggtt gagaaagacg gtaaccgcta tgcattttcc 2881 agtgaatata gcgacctacc aatcgcaaca cctggtacag ggcctacaga tccggtaact 2941 agctgtaaag gtacacctat cagtcaaatt gtggtttacc cgaacctacc acagggcacg 3001 catgctgctc aaggtgattt aattgttgaa ggaaacgcgg tgtatgaagc gaaatggtgg 3061 agcaataagc aaccatcagt tagcggcgat tataccaagg cttgcaactt gtaaataata 3121 aatggggagc tttgctcctt ttataaaagg cgaataagca tgaatagttt acaccgtaaa 3181 tatgcgcttg gtctttttgg cgcaacctca gctttgattt caagcgcagt ttcagctcat 3241 ggttttatgg actttcccaa agcaaggcag gcgatctgtc aagcgcaagg aggttattgg 3301 tggccggatg atggatcaaa tatcccaaac ctagcatgtc gagcggcttt tctagactca 3361 ggccattttc agtttgtgca agagcatgaa ttttcgacca ataccgctga ctacaataat 3421 ctagaggcag taaaagcgaa tatccccaat ggtacgcttt gctcagctgg tgacccaaac 3481 aaaagtggca tgagtgttgt ctcaccacac tggcagcgta cagttgtaga gccaaacgct 3541 caaaataaac ttgccattag ctttagggcg acaacacctc ataatcccag tttttggcag 3601 ttcttcttgt caaagccaaa ctacgatggc gatacatctc ctctgacttg gaatgattta 3661 gagcttatta atgaatttgg caatattgat ttcgttgtgg atcctgaagg taaacgcttc 3721 tataaaatgg aaatagatat accgcaaggc cgtgagggca atgcagtttt atatacccgt 3781 tggcagcggt ttgatgtcgc tggagaggga ttttacaatt gctctgatat cacaataacc 3841 tcaacaggca cgccaccaga atgggtgagc agcggctatt tcttaaagca agggcaagat 3901 gctaaagccg gtgatagcgt gtggtttagg acgtttgatc aatccgggaa tgaattaatt 3961 agccataact tgaatgtaac ctccactaat gcatcaacat ggcaagctga gttggctctt 4021 taccttaata gcaattacgg aaataaggtc aacattggtg ttaagcaacc ggacggttca 4081 attgcgtttg atgcaaacgc cttattagca aacgaagtat ttttgccaca tgccgcttac 4141 accagcaatc ttagcgttgt aaaagggggt gacaacaccg ctcctacggt aaatccaatt 4201 tctaaccaaa gcatcgcaga aaatagccaa gcgacggtgc atgctcatgc gtttgacgac 4261 caaaatgacc cactaacatt catttggagt attcctgcac cgcttagctt tagtggcgaa 4321 ggtgacacga tttccatctc aacacctgaa gtgacggctg atcaaacctt tgaggctgaa 4381 cttaatgtca ccgatggcaa agcaagcacg accgtaagtt tctcaattac cgttcaagat 4441 agcaccaata accaataccc agcttggaat agtacaacta cctatgtcgc aggcgacaga 4501 gtaacgcatc aacagaaagt gtatgaagct aagtggtgga cgcagggaga agagcccggc 4561 gcatccgatg tttggaaagc aatttaaaga aaacccaaat taattgggta caacatgtaa 4621 ctacaataag gttgaagata tgaaacttaa taaaataacc agctatatag gatttgcctt 4681 actgagtggc ggggcacttg cagccccttc aacaccaaca ttagattggc agccacaaca 4741 gtattcgttc gttgaagtta acgtagatgg gcttggctct tacaagcaac tggtaaaagc 4801 caaagatgtt gtcgatatta gcatcaaatg gaatgcatgg agtggctctg gcggtgataa 4861 ctataaagta tattttgatg atctcctcgt aaaccaaggc agcttgccag ctggtactaa 4921 aagtggggta gtacaattcc cctacactaa atcgggtcgc catcagcttt atttagaact 4981 gtgtgaaggt accgtatgtg caagaagcgc agggaaagaa atcgtcattg ccgacactga 5041 cggtgcacat ctagcgccac ttcctatgaa tgtagatccg aacaaccgta ataacggtac 5101 aataccaggc cgtgtaacgg gtgcatattt cgtcgaatgg ggcatatatg gccgtaatta 5161 tgacgtaact aagatcccag ctcataactt atcgcacatt ttatatggct ttattccgat 5221 ttgcggacct aacgaatcac tcaaatccat cgaaattggt aatagttgga gagcgcttca 5281 aactgcatgt gctgactccc aagattatga agtcgtcatt cacgaccctt gggctgcagt 5341 gcaaaaatct atgccagggg tggatgcaaa agatcctatt cgcggtgtat attctcaatt 5401 aatggcgcta aaacaacgtt atccggacct taaaattctg ccttcagtcg gtggatggac 5461 gctatctgac ccattccatg gctttacaaa caaagcaaac cgcgacactt tcgtggcgtc 5521 tgtaaaacaa tttcttaaaa cgtggaagtt ttatgacggt gtagatatcg actgggaatt 5581 cccaggtggt gatggtccaa acccagactt gggcgatcca attaacgacg gtccagcata 5641 tgtggcgcta atgcaagaac ttcgagcaat gctggatgag ttagaggctg aaaccggacg 5701 tcaatatgag ctcacttctg caataggtgc gggttatgac aagattgaag atgtagatta 5761 ccaagctgcc caacagtata tggattatat ctttgcgatg acctatgact tctatggtgc 5821 ttggaacaat gaaacgggtc atcaaaccgg tatctattgt ggctctcatc tcagcacaga 5881 cgaatgtaac ggtacaggcg ttgacgacaa tggagtgcca cgtaaaggcc cagcatatac 5941 tggcgaccat gcaattcagt tgttacttca acaaggtgtg cagccctcta agctcgttat 6001 gggcgttgca atgtacggcc gcggctggga aggtgtgcta gatgcaaatg cagccattcc 6061 aggtaatccg atgacggcgc cgggtaatgg cccattgaca ggttctacaa gtgaaggtgt 6121 ttgggagccg ggcattatgg attacaaggc tattgcagca aacgctgtag gccaaggtgg 6181 ttcaggcgtt aatggttatg aagtaggcta cgatgagcaa gcacaagcag catatgtttg 6241 gaacagaagt aacggtaaac tcatcactta cgatagccca cgcagcgtta tcgcaaaagg 6301 ccagtatgca aacactcatc aactagctgg tttatttggt tgggaaattg acgctgataa 6361 tggcgacatt ctaaatgcaa tgtatgatgg tcttacagca ggtgaaatcc ctaatcgcgc 6421 tcctactatc ggggtttctg gaccaatcaa tgtgacctca ggtcaggttg tgaacgtcga 6481 tgcgcaagca agcgatttag acaatgatcc attaacctat tcatgggttg ctgcgcctgg 6541 tttggctttg tctgctaaca acaccgcagc tgtggctgta actgctcctt cagtcgctca 6601 acagacaagt tacgacttga ctgtaacggt caatgatggg gcgttatcaa caaccaaaac 6661 aatcgttgtt gttgtaaacc cagaaggtgc aaatgcagcg cctgtcgtta caccggtttc 6721 agatatttcg gttaacgaag gggcctcggc tactgtcaat gtgtcagcaa ctgaccctga 6781 aggcgcagca cttagctata gctggagtgt accagccgag ctaagcgtag caaatggtag 6841 ctcagcgact attactgctg caaatgtcac agccgacaca accgtcccgg taactgttac 6901 cgtatctgac ggcgttaacg ctgttgacac aacatttaat gtcacgatta aagacggtgc 6961 tgaatatcct acttgggatc gatcaacagt ttatgtcggt ggcgaccgag taattcataa 7021 cagcaatgtc tttgaagcaa aatggtggac tcaaggtgaa gaacctggaa ccgcagatgt 7081 atggaaggca gtaactaact aattcataac agcaatgtct ttgaagcaaa atggtggact 7141 caaggtgaag aacctggaaa ccgcagatgt atggaaggca gtaactaact aatctaacca 7201 tccccaatta atgagcttgc cagtcaagct ca //