APIS families if_verified APIS genes Defense systems References Reference link Representative protein protein link Family HMM Phage Genome accession Host taxonomy Host TaxID domain phylum class order family genus species Pubmed/Publication Pubmed/Publication link PDB structures Description Pfam domains Representative protein sequence start end strand neighbor_start neighbor_end #ofmembers Seq length(aa) Isoelectric point Molecular weight Charge ptm clan_system clan ID clan_seed_family PHROG APIS001 1 Apyc1 pyrimidine cyclase system for antiphage resistance (Pycsar) Hobbs et al., 2022 https://www.nature.com/articles/s41586-022-04716-y YP_009840594.1 https://www.ncbi.nlm.nih.gov/protein/YP_009840594.1 APIS001.hmm Bsp38 of Bacillus subtilis GCF_003367035.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:35395152 https://pubmed.ncbi.nlm.nih.gov/35395152/ 7T28,Bsp38;7U2R,Paenibacillus J14;7U2S,Paenibacillus xerothermodurans SBSphiJ Apyc1 efficiently hydrolyses a wide range of cyclic mononucleotides; 273 Acb1 and 107 Apyc1 phage proteins were identified. PF00753.30 MLHTTQIRMVGTGSAFSKKFYNNSALVTFTNGYNLLIDCGHSVPKGLHDADIPLESIDGILITHTHADHIGGLEEVALYNKFVLGGRKIDLLVPNTLVESLWENSLKGGLRYSDTYDDLSLSDYFTVRSLKTFTSGAARTQLEENIAIKLYPTFHVSHMASYAVGLEDRGEDKVFYSSDTIFDEYLIDYALTYSWVFHDCQFFTGGVHASLDELLNYIPEEDQDRVFLMHYGDNMEDFFTKTGRMRFALQGRTYIL 92525 93295 + 90711 94446 26 256 4.8545 29051.70 -9.5 0.89 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS002 1 vs.4 cyclic oligonucleotide-based antiphage signaling system (CBASS) Jenson et al., 2023 https://www.nature.com/articles/s41586-023-05862-7 NP_049728.1 https://www.ncbi.nlm.nih.gov/protein/NP_049728.1 APIS002.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:36848932 https://pubmed.ncbi.nlm.nih.gov/36848932/ 7UQ2 Vs.4 antagonized cGAS signaling by binding tightly to cGAMP (dissociation constant of approximately 30 nM) and sequestering it; Bioinformatic analyses identified 198 homologues of Vs.4 in diverse phages; belongs to PHROG 717. MIEDIKGYKPHTEEKIGKVNAIKDAEVRLGLIFDALYDEFWEALDNCEDCEFAKNYAESLDQLTIAKTKLKEASMWACRAVFQPEEKY 63078 63344 - 60922 65355 103 88 4.3805 10210.61 -6.5 0.7 cyclic oligonucleotide-based antiphage signaling system (CBASS) CLAN056 APIS002 phrog_717,phrog_26803 APIS003 1 ArdA restriction-modification (RM) Belogurov et al., 1985 https://link.springer.com/article/10.1007/BF00332948 AAB36891.1 https://www.ncbi.nlm.nih.gov/protein/AAB36891.1/ APIS003.hmm plasmid pKM101 of E. coli U72482.2 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:2989658 https://pubmed.ncbi.nlm.nih.gov/2989658/ 2W82 DNA mimetics, i.e. structurally and electrostatically imitate the B-form of DNA and thus function as competitive inhibitors of restriction enzymes. PF07275.14 MTDITTPSVYVGTYHKYNCGSIAGAWLDLTDFDSSEEFYERCRELHANEADPEFMFQDWEGIPSDMASECHINWDFINGFKQAREEGNEAAFVAFVDLFNSTDFDLFRDAYMGEAKDEETFAEEYLNDSGLLNEIPESVARYFDIVAYARDLFIGDFSLHDGHVFNMTC 207 169 3.8197 19427.22 -24.5 0.83 restriction-modification (RM) CLAN005 APIS003,ardu phrog_2059 APIS004 1 Acb2 cyclic oligonucleotide-based antiphage signaling system (CBASS) Huiting et al., 2023 https://www.sciencedirect.com/science/article/pii/S0092867422015847?via%3Dihub ANA48877.1 https://www.ncbi.nlm.nih.gov/protein/ANA48877.1/ APIS004.hmm PaMx33 of Pseudomonas aeruginosa GCA_002757475.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:36750095 https://pubmed.ncbi.nlm.nih.gov/36750095/ 8H2X "Anti-CBASS protein (""Acb2"") that forms a hexamer with three 3',3'-cGAMP molecules and reduces phospholipase activity." MDNQHKKIKGYRDLSQEEIDMMNRVKELGSQFEKLIQDVSDHLRGQYNASLHNRDEITRIANAEPGRWLAIGKTDIQTGMMAIIRAIAQPDSF 24098 24379 - 12321 29731 206 93 6.7258 10699.13 0.5 0.79 cyclic oligonucleotide-based antiphage signaling system (CBASS) CLAN036 APIS004 phrog_29522,phrog_717 APIS005 1 Gad1 Gabija Yirmiya et al., 2023; Antine et al., 2023 https://doi.org/10.1101/2023.05.01.538930;https://doi.org/10.1101/2023.05.01.538945 APD21271.1 https://www.ncbi.nlm.nih.gov/protein/APD21271.1/ APIS005.hmm phi3T of B. subtilis GCA_002601445.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis Yirmiya et al., 2023; Antine et al., 2023 https://doi.org/10.1101/2023.05.01.538930; https://doi.org/10.1101/2023.05.01.538945 Gad1 forms an octameric web that encases the GajAB complex and inhibits DNA recognition and cleavage. MKLIGIKTSNCFLVSDNIEGKRYFHSQLDELLFDGKRATETYKSDWFKLEKEPSVIEKQMPAKKINHRYELKEGFQESELTPKVIKASYIGEDSEYYEVKGLYDLKFEEIPQQNEKIEFEMNVIEEIDGELKLQSHNFNLNYNLLDRIQTHPMLLETKPCYLSQEESYKIIRNHIKANINPKFARITSDYDFCLTVVKVLELYKPHEYIVDLNAMYKRRKPKLEKRFQTKREVEIYKVAPKAYQSYPIVEPFSGKDVEDLKSNIKKFLDDLMAKINEPLVECKCCKGRGVILNEN 87269 88156 + 85148 90075 29 295 7.0151 34865.17 2.0 0.61 Gabija CLAN009 APIS005 phrog_20113 APIS006 1 abc1 RecBCD Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full AXC42625.1 https://www.ncbi.nlm.nih.gov/protein/AXC42625.1/ APIS006.hmm Salmonella phage S149 GCA_003342335.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Salmonella; 590 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Trigger programmed cell death (PCD) by P4 prophage. PF08281.15 MRRLNITPAEMESVCGRMVACRAAEHLGLNINQFYYIAKKLSLKTAFVKPRWSDDEDKRMQTLISSGYTQRNVAKILGRSEESVKSRLSRLRKK 7155 7439 - 5267 9002 37 94 10.9204 10876.71 11.5 0.56 RecBCD CLAN037 APIS006 phrog_323 APIS007 1 KlcA restriction-modification (RM) Serfiotis-Mitsa et al., 2010 https://academic.oup.com/nar/article/38/5/1723/3112516 BAF33451.1 https://www.ncbi.nlm.nih.gov/protein/BAF33451.1/ APIS007.hmm IncP-1b plasmid of Bordetella pertussis AB237782.1 d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Alcaligenaceae;g__Bordetella;s__Bordetella pertussis 520 Bacteria Proteobacteria Betaproteobacteria Burkholderiales Alcaligenaceae Bordetella Bordetella pertussis PMID:20007596 https://pubmed.ncbi.nlm.nih.gov/20007596/ 2KMG ArdB and KlcA act as anti-restriction proteins and inhibit the four main families of Type I RM systems in vivo. PF03230.16 MNTEEQPVTASLVAEAQRLDFLPTYFGPRLMMRGEALVYAWMRRLCERYNGAYWHYYALSDGGFYMAPDLAGRLEIEVNGNGFRGELSADAAGIVATLFALGQLAAEIADTDAADALIDRYHFLRGFAAGHPEAAAIYRAID 63 142 4.4687 15659.69 -6.5 0.76 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS008 1 Gad2 Gabija Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 Gad2 APIS008.hmm SPbetaL7 of B. subtilis d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 Alphafold2 predicted that Gad2 is an enzyme with a nucleotidyltransferase protein domain, suggesting that it inhibits Gabija via a mechanism of action different than Gad1. MSYQFEKNKLYAYLGEELVEALKRNEAIIAGGAITSLFNNKEINDVDIYFRSDKKACSFLEECWNSNVYVTSHTKKATLFIKKRLKLQMIHFKFFSDAESIFNTFDFTVCMGAFDFKTEAFTLHEDFLKHNSQRILKFNSQTAFPIVSLLRVQKYTDKEYTISKPEFIRIVLTCMDLTINTYEELKDQMGGMYGINYDKLFEDEKDEDFNLREAVDKIADMVLDEDYFKEPVNLEFNDLDDLLNDINKSPVMTLKINDDQYRIGLDGFLKESVSAPCTEIKLDTKDFFDKTNFYKFVRKQNGKLTSFYDKNFEYVIGEEAKAEGVIDSWSNSGKLFFNEKAAIEQSTYYGKEDGVLIEVKIKEKDFVDADNGKVEATACQVIREVSKDEWKEYISANNSK 49 400 4.6283 46527.64 -16.0 0.71 Gabija CLAN023 APIS008 phrog_18122,phrog_27100,phrog_4400,phrog_25514,phrog_37109,phrog_16176 APIS009 1 Ral restriction-modification (RM) Loenen & Murray, 1985 https://www.sciencedirect.com/science/article/abs/pii/0022283686900719 NP_040622.1 https://www.ncbi.nlm.nih.gov/protein/NP_040622.1/ APIS009.hmm Lambda of E. coli GCF_000840245.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:3023633 https://pubmed.ncbi.nlm.nih.gov/3023633/ Ral of phage λ can activate the activity of the MTase and thereby accelerate protection of the phage DNA. PF11058.11 MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVDKWAIRTTAMIARELGKQNNKAA 34087 34287 - 32025 35036 22 66 8.5100 7603.86 3.0 0.53 restriction-modification (RM) CLAN057 APIS009 phrog_1055,phrog_17610 APIS010 1 Ocr restriction-modification (RM);bacteriophage exclusion (BREX) Isaev et al., 2020; Krüger et al., 1977; Atanasiu et al., 2002; Walkinshaw et al., 2002 https://academic.oup.com/nar/article/48/10/5397/5825620;https://link.springer.com/article/10.1007/BF01036001;https://academic.oup.com/nar/article/30/18/3936/1075436;https://www.sciencedirect.com/science/article/pii/S1097276502004355 NP_041954.1 https://www.ncbi.nlm.nih.gov/protein/NP_041954.1/ APIS010.hmm T7 of E. coli GCF_000844825.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:32338761;329108;12235377;11804597 https://pubmed.ncbi.nlm.nih.gov/32338761/;https://pubmed.ncbi.nlm.nih.gov/329108/;https://pubmed.ncbi.nlm.nih.gov/12235377/;https://pubmed.ncbi.nlm.nih.gov/11804597/ 1S7Z Ocr physically associates with BrxX methyltransferase to neutralizes their ability to both methylate and exclude incoming phage DNA. Ocr structurally mimics the DNA phosphate backbone and interacts directly with EcoKI (both the MTase and REase domain of this type I R–M enzyme), thereby interfering with the activity of this system. PF08684.13 MAMSNMTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHMAADNAVPHYYADIFSVMASEGIDLEFEDSGLMPDTKDVIRILQARIYEQLTIDLWEDAEDLLNEYLEEVEEYEEDEE 925 1278 + 925 3100 42 117 3.6438 13809.08 -26.0 0.85 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_2568 APIS011 1 gp4.5 toxin-antitoxin (TA) Sberro et al., 2013 https://www.sciencedirect.com/science/article/pii/S1097276513001299 NP_041980.1 https://www.ncbi.nlm.nih.gov/protein/NP_041980.1/ APIS011.hmm T7 of E. coli GCF_000844825.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:23478446 https://pubmed.ncbi.nlm.nih.gov/23478446/ Protein gp4.5 allows T7 to block Lon (protease) from degrading antitoxins (the SanTA system in E. coli), including SanaA, thereby preventing liberation of SanaT. However, beyond a possible interaction between gp4.5 and Lon in uninfected cells, this speculative model remains untested. PF17574.5 MSNVAETIRLSDTADQWNRRVHINVRNGKATMVYRWKDSKSSKNHTQRMTLTDEQALRLVNALTKAAVTAIHEAGRVNEAMAILDKIDN 13584 13853 + 11565 17359 43 89 10.5538 10091.46 5.5 0.68 toxin-antitoxin (TA) CLAN024 APIS011 phrog_2254 APIS012 1 tifA toxin-antitoxin (TA) Srikant et al., 2022 https://elifesciences.org/articles/79549 NP_049652.1 https://www.ncbi.nlm.nih.gov/protein/NP_049652.1/ APIS012.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:35924892 https://pubmed.ncbi.nlm.nih.gov/35924892/ Original gene 61.4, experimentally evolved T4 phage to overcome a phage-defensive toxin-antitoxin system (toxIN) in E. coli. Through recombination, T4 rapidly acquires segmental amplifications of a previously uncharacterized gene, now named tifA, encoding an inhibitor of the toxin, ToxN. MHIVLFKPTPYNVRKNTQFKALIADTWELVLDIPAEESPPFGRVEFIKFAVRPTKRQIRQCKRYFRKIVKLEKQFVTCDYAEILK 20112 20369 - 16846 24235 73 85 10.4193 10187.10 8.5 0.57 toxin-antitoxin (TA) CLAN038 APIS012 phrog_8921,phrog_2375,phrog_3272,phrog_12748 APIS013 1 Dmd toxin-antitoxin (TA) Otsuka & Yonesaki, 2012 https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2012.07975.x NP_049653.1 https://www.ncbi.nlm.nih.gov/protein/NP_049653.1/ APIS013.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:22403819 https://pubmed.ncbi.nlm.nih.gov/22403819/ 5I8J T4 produces Dmd, an antitoxin that inhibits E. coli RnlA and LsoA toxins. Dmd differs from the RnlB or LsoB antitoxins, suggesting it evolved independently, which is highlighted by its different toxin neutralization mechanism. PF17587.5 MELVKVVFMGWFKNESMFTKEITMMKDDVQWATTQYAEVNKALVKAFIDDKKVCEVDCRG 20371 20553 - 17935 25455 74 60 4.9813 7027.25 -1.0 0.77 toxin-antitoxin (TA) CLAN058 APIS013 phrog_2102 APIS014 1 PinA toxin-antitoxin (TA) Hilliard et al., 1998 https://www.sciencedirect.com/science/article/pii/S0021925818386101 NP_049694.1 https://www.ncbi.nlm.nih.gov/protein/NP_049694.1/ APIS014.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:9417110 https://pubmed.ncbi.nlm.nih.gov/9417110/ PinA encoded by T4 was discovered 20 years ago, it could help T4 block TA defense systems that require Lon, but in vivo function for pinA has not been reported. PF10465.12 MITVDKWFRINRADTGLCNYWPELSAGTVFKVRELVKECEDDIEPDTGIIEIELSDGKIINIYDKPITYWCLWNTESVENGEIEEVVERTNQVVQKPKADFQGERISYALAKLAAQENNDGYEGNLMQAAAEYIEWLETQISFSDRMIQQYKRLHQMFYNT 46897 47382 - 43535 48635 191 161 4.1965 18816.14 -12.5 0.52 toxin-antitoxin (TA) CLAN059 APIS014 phrog_2167,phrog_9092 APIS015 1 IpII restriction-modification (RM) Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full NP_049734.1 https://www.ncbi.nlm.nih.gov/protein/NP_049734.1/ APIS015.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full T4 internal-protein, a T2-restricting defense system was disabled by IpII. IpII restored T2 and T6 plaquing but did not further enhance T4 infection. MKTYQEFIAEARVGAGKLEAAVNKKAHSFHDLPDKDRKKLVSLYIDRERILALPGANEGKQAKPLNAVEKKIDNFASKFGMSMDDLQQAAIEAAKAIKDK 65416 65718 - 63381 68319 34 100 10.0101 11085.80 5.0 0.57 restriction-modification (RM) CLAN060 APIS015 phrog_7857 APIS016 1 Acb1 cyclic oligonucleotide-based antiphage signaling system (CBASS) Hobbs et al., 2022 https://www.nature.com/articles/s41586-022-04716-y NP_049750.1 https://www.ncbi.nlm.nih.gov/protein/NP_049750.1/ APIS016.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:35395152 https://pubmed.ncbi.nlm.nih.gov/35395152/ 7T26 Acb1 rapidly degrades the CBASS signals 3′3′-cGAMP, cUA and cAAA, but does not cleave cGG. MMEFKDFSTGLYVAAKFSELTLDALEELQRSLRVPNPVPREKIHSTICYSRVNVPYVPSSGSFEVASSGHLEVWKTQDGSTLVLVLDSEYLRCRHMYARALGATHDFDDYTPHITLSYNVGPLSFSGDVQIPVVLDREYKEPLKLDWADDLK 73952 74410 - 71253 77337 41 152 4.9749 17244.53 -4.5 0.85 cyclic oligonucleotide-based antiphage signaling system (CBASS) CLAN061 APIS016 phrog_755 APIS017 1 Alt toxin-antitoxin (TA) Alawneh et al., 2016 https://onlinelibrary.wiley.com/doi/10.1111/mmi.13225 NP_049811.1 https://www.ncbi.nlm.nih.gov/protein/NP_049811.1/ APIS017.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:26395283 https://pubmed.ncbi.nlm.nih.gov/26395283/ T4 protein Alt (an ADP-ribosyltransferase) is injected with phage DNA, which chemically modifies the MazF toxin. ADP-ribosylated MazF has reduced cleavage activity, enabling the survival of the phage. PF03496.17 MELITELFDEDTTLPITNLYPKKKIPQIFSVHVDDAIEQPGFRLCTYTSGGDTNRDLKMGDKMMHIVPFTLTAKGSIAKLKGLGPSPINYINSVFTVAMQTMRQYKIDACMLRILKSKTAGQARQIQVIADRLIRSRSGGRYVLLKELWDYDKKYAYILIHRKNVSLEDIPGVPEISTELFTKVESKVGDVYINKDTGAQVTKNEAIAASIAQENDKRSDQAVIVKVKISRRAIAQSQSLESSRFETPMFQKFEASAAELNKPADAPLISDSNELTVISTSGFALENALSSVTAGMAFREASIIPEDKESIINAEIKNKALERLRKESITSIKTLETIASIVDDTLEKYKGAWFERNINKHSHLNQDAANELVQNSWNAIKTKIIRRELRGYALTAGWSLHPIVENKDSSKYTPAQKRGIREYVGSGYVDINNALLGLYNPDERTSILTASDIEKAIDNLDSAFKNGERLPKGITLYRSQRMLPSIYEAMVKNRVFYFRNFVSTSLYPNIFGTWMTDSSIGVLPDEKRLSVSIDKTDEGLVNSSDNLVGIGWVITGADKVNVVLPGGSLAPSNEMEVILPRGLMVKVNKITDASYNDGTVKTNNKLIQAEVMTTEELTESVIYDGDHLMETGELVTMTGDIEDRVDFASFVSSNVKQKVESSLGIIASCIDIANMPYKFVQG 123454 125502 - 120659 128765 281 682 6.2337 75817.56 -1.5 0.67 toxin-antitoxin (TA) CLAN062 APIS017 phrog_26651,phrog_15247,phrog_23015,phrog_802,phrog_15144,phrog_23966 APIS018 1 Arn restriction-modification (RM) Ho et al., 2014 https://www.sciencedirect.com/science/article/pii/S0021925820371635 NP_049868.1 https://www.ncbi.nlm.nih.gov/protein/NP_049868.1/ APIS018.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:25118281 https://pubmed.ncbi.nlm.nih.gov/25118281/ 3WX4 T4 phage protein Arn (Anti restriction nuclease) was identified as an inhibitor of the restriction enzyme McrBC. MIIDSQSVVQYTFKIDILEKLYKFLPNLYHSIVNELVEELHLENNDFLIGTYKDLSKAGYFYVIPAPGKNIDDVLKTIMIYVHDYEIEDYFE 161312 161590 - 156542 163602 70 92 4.2444 10900.49 -7.5 0.72 restriction-modification (RM) CLAN063 APIS018 phrog_3151 APIS019 1 gnarl2 O-antigen-based barrier Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full NP_050611.1 https://www.ncbi.nlm.nih.gov/protein/NP_050611.1/ APIS019.hmm Escherichia phage Mu GCF_000837225.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Putative O-antigen modifiers, interfere with O-antigen biosynthesis. MAKVIIEIKNTVSGIKGRNLRTSIAVDGSAELDGDEGTLAGMVALLVLNKSQKIINESAHEAIEILKNDGVITSGRVTEMAVEKTCH 4784 5047 + 1099 7484 28 87 5.6793 9217.64 -1.0 0.62 O-antigen-based barrier CLAN064 APIS019 phrog_10252 APIS020 1 Abc2 RecBCD Murphy, 2000; Wilkinson et al., 2022 https://www.sciencedirect.com/science/article/pii/S0022283699934861;https://elifesciences.org/articles/83409 NP_059594.1 https://www.ncbi.nlm.nih.gov/protein/NP_059594.1/ APIS020.hmm Salmonella phage P22 GCF_000845765.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Salmonella; 590 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella PMID:10669596;36533901 https://pubmed.ncbi.nlm.nih.gov/10669596/;https://pubmed.ncbi.nlm.nih.gov/36533901/ 8B1T Bacteriophage P22 Abc2 protein binds to the RecBCD enzyme from E. coli to promote phage growth and recombination; the RecBCD-Abc2 structure shows that Abc2 binds to the Chi-recognition domains of the RecC subunit in a position that might enable it to mediate the loading of phage recombinases onto its single-stranded DNA products. PF11043.11 MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEKEFRHCIWLAEKEERERIYQTAIRPFRKATYTKFIEIDPRLRDYRSRYGAISNN 26630 26923 - 25964 25967 65 97 8.9809 11620.18 3.5 0.36 RecBCD CLAN065 APIS020 phrog_656 APIS021 1 SieA restriction-modification (RM) Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full NP_059639.1 https://www.ncbi.nlm.nih.gov/protein/NP_059639.1/ APIS021.hmm Salmonella phage P22 GCF_000845765.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Salmonella; 590 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Superinfection exclusion. MSDSMSYAVLVAATLFLGIGLQIAWLFFSNFIKRKRLESRISEVSIAIGKNAKNPENEAYVLNYLKEKFSPERFENRITDALGLIISVIHIPLSLLITVWYFAMIAGRIFGFMNIEPVVLWVPMILQLLLSIAIFIFSVFIKIVFGRYPGEANGFNKEFIKTIK 13336 13830 - 10069 13313 32 164 10.0689 18701.33 5.5 0.55 restriction-modification (RM) CLAN039 APIS021 phrog_7745,phrog_10966 APIS022 1 adfA toxin-antitoxin (TA) LeRoux et al., 2021 https://www.nature.com/articles/s41564-022-01153-5 NP_861724.1 https://www.ncbi.nlm.nih.gov/protein/NP_861724.1/ APIS022.hmm RB69 of E. coli GCF_000858005.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:35725776 https://pubmed.ncbi.nlm.nih.gov/35725776/ Gene adfA(gp61.2) is encoded as a DarT (TA system) inhibitor. MHKNAPFKYGKFPNAQCYNITPNENNNGYHIGVIFVIVKDNEIVAWADFKGTTYDVNPVPFTYYNIMDLAYDYNWFNHDTLAHIEGVGFDISYSSYSLCPMSRAHGKDASYLSIRKRVNFKRSTEYVGGLFVKDNKITRISYPLSVSQKDVDVDLDLTENNINRIASVYFDIDEKIVVCGYELPPEEKAEAIEVELEISVDDQIFNAFMNRG 18002 18640 - 16061 20155 176 212 4.6927 24319.32 -7.5 0.43 toxin-antitoxin (TA) CLAN066 APIS022 phrog_3781,phrog_8539,phrog_4122 APIS023 1 gp5.9 RecBCD Wilkinson et al., 2022 https://elifesciences.org/articles/83409 NP_041987.1 https://www.ncbi.nlm.nih.gov/protein/NP_041987.1/ APIS023.hmm T7 of E. coli GCF_000844825.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:36533901 https://pubmed.ncbi.nlm.nih.gov/36533901/ 8B1R Gp5.9 completely inhibits RecBCD by preventing it from binding to DNA. The RecBCD-gp5.9 structure shows that gp5.9 acts by substrate mimicry, binding predominantly to the RecB arm domain and competing sterically for the DNA binding site. MSRDLVTIPRDVWNDIQGYIDSLERENDSLKNQLMEADEYVAELEEKLNGTS 24 52 3.8516 6044.63 -8.0 0.57 RecBCD CLAN067 APIS023 phrog_9014 APIS024 1 Tad1 Thoeris Leavitt et al., 2022 https://www.nature.com/articles/s41586-022-05375-9 UPI13470.1 https://www.ncbi.nlm.nih.gov/protein/UPI13470.1/ APIS024.hmm SBSphiJ7 of B. subtilis GCA_023539245.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:36174646 https://pubmed.ncbi.nlm.nih.gov/36174646/ 7UAV Tad1 proteins are not an enzymes, but are 'sponges' that bind and sequester the immune signaling molecule (cyclic ADP-ribose or gcADPR) produced by TIR-domain proteins, thus decoupling phage sensing from immune effector activation and rendering Thoeris inactive. MRELKHELLPTRHTQVFHEDKDKMEFNAPHHFVVVPAGSPLVDQQIHYGRGGNSKKVTVKDFAGKLATVNFQLGPVTEHGANGVMNEDLIAMVITRLQYFQNSEFNCRENAMAITKLEEALMWLNKRTAEREQRGVEGTHEK 229 142 7.1870 16211.44 3.0 0.75 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2428 APIS025 1 Had1 Hachiman Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 UPI12729.1 https://www.ncbi.nlm.nih.gov/protein/UPI12729.1/ APIS025.hmm SBSphiJ4 of B. subtilis GCA_023539215.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 Had1 (Hachiman anti-defense 1) is a Hachiman-inhibiting family of phage proteins. The mechanism of Hachiman defense is unknown. MEEFKMTVWTNGKAIRKYTGQDKHPDTNPSKRMEWFKATAIIKPDTGDNNERD 151219 151380 + 148996 153148 4 53 8.6196 6228.98 1.5 0.31 Hachiman CLAN068 APIS025 phrog_5701 APIS026 1 orf126 broad-spectrum counter-defense Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full VFR14542.1 https://www.ncbi.nlm.nih.gov/protein/VFR14542.1/ APIS026.hmm Salmonella phage SPFM20 GCA_005410195.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Salmonella; 590 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Broad-spectrum counter-defense. MTIDYRRTYFFDTKRKVNNIISGIASLDIMIYAIQGKAGKEVLAEILSERNIAIPPTLRCLTPEELKALAFVVCKSQLKTTTVGGRMKVVLRVLLLITH 22169 22468 + 20175 26471 3 99 10.3434 11122.34 7.5 0.24 broad-spectrum counter-defense CLAN069 APIS026 phrog_8261 APIS027 1 DSAD1 defence-associated sirtuin (DSR) Garb et al., 2022 https://www.nature.com/articles/s41564-022-01207-8 WP_004399562.1 https://www.ncbi.nlm.nih.gov/protein/WP_004399562.1/ APIS027.hmm SPbeta phages of B. subtilis d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:36192536 https://pubmed.ncbi.nlm.nih.gov/36192536/ DSAD1 is a phage anti-DSR2 protein that binds and inhibits Defence-associated sirtuins (DSR2). In bacteria, N-terminal sirtuin (SIR2) domains within DSR2 were shown to participate in defence systems including prokaryotic argonautes (pAgo), Thoeris, AVAST, defence-associated sirtuin (DSR), etc. MIEIFKDTGATHDLVYHSKINTFVWDVEFDIVLSDSKELNKCYFVKCFNPYRINGKCDFAVSSIDIFSEGKRLLIENEFNFKITKAVHVATSKDVTEIVLHLSERISSPFPIVKEVVYLD 4 120 5.5991 13854.95 -2.0 0.25 defence-associated sirtuin (DSR) CLAN070 APIS027 phrog_37006 APIS029 1 Tad2 Thoeris Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 YP_002300464.1 https://www.ncbi.nlm.nih.gov/protein/YP_002300464.1/ APIS029.hmm Bacillus phage SPO1 GCF_000881675.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus; 1386 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus Yirmiya et al., 2023 https://doi.org/10.1101/2023.05.01.538930 "Tad2 is a ""sponge"" that sequesters the immune signaling molecules produced by Thoeris TIR-domain proteins in response to phage." PF11195.11 MKTKMSFGEALEVLKQGMQVYRSGWNGKNMFLFLKSSDALASDFGFGFGEYINEPVFGNIIFIKTADNKIHAWVPSQTDVLAEDWDIVS 127121 127390 + 125619 128593 132 89 4.6684 10029.45 -2.5 0.79 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2709,phrog_30130,phrog_872,phrog_1048 APIS030 1 DarB restriction-modification (RM) Lida et al., 1987 https://www.sciencedirect.com/science/article/abs/pii/0042682287903242 YP_006479.1 https://www.ncbi.nlm.nih.gov/protein/YP_006479.1/ APIS030.hmm P1 of E. coli GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:3029954 https://pubmed.ncbi.nlm.nih.gov/3029954/ Bind to phage DNA, thereby masking type I R–M recognition sites and preventing degradation of phage DNA. PF02384.19,PF00176.26,PF00176.26,PF00271.34 MNKLSMGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYDRLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVWEIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQISPFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGGFMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHPAEMAEKIPLVHESTLESANVLWPTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQIDNQALKAKLIHCFESRIDWYLLDMAEPSPTADVVDEGEMRLINGVWQKYAGGRWIESDAGKELKIDVASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLVDWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRFGNPGRGPIAKLSGSGARAWFAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTLRYLYSDLTRDPIQLDDFRLAFTGELPASDDELLNLLASTPGIAVSPYGGIVPFARATSGDINEIVAPKQEFLATLTDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSVILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRYEWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRYDELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGTGLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYYEAKEFLSEEAFSNYLFVGLDVLMDGDQIRQVPVLDENGKPVLGTDGTPVMRDALKLADEATITARMNAIPHSNYRAVVFTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDTGTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIKNAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQKMGIYGPDDFVKFFGKTRLETVQKISGEVEEKMALVGFENLDALRGIFHRWTTLKTAEDVKDTVEIPELDEHQQDAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVCTDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEFIQLQVPEAFEQEVNKRLARFGIDEQTVTHPVTPKYAKLIATLKEFFPEGKQIIFTDEKTQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIAKYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDIHHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDILRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQNYIKAQHAAGEDVEVLTAELERSKAELEKTTAEVAKFKQAVMAKAADNADWKARWGSVHHTDRMLLAQYRASLKSAIQRKANISQAISPYEKLLNRTQKAATDIKRLRPLVEDAINKGILDVDPDLVNHASEFLVIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTFKGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIKITDSYGVQTTESGYSIDFVGTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQVRLAEVFLTELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSELEVTNAIFGTHEFRASDYEITTAQFGTIGIYSNKAEIKQAMDAASARIAAEREANLNHAVAALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRMTYNLVSMFSDLAILGLVDLNEVTPELLSMRKNHVEILQRINTVLAGRTDEEKQADADRINLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAAGEVFGLQEASGMKGVLFRAKDAIKTKFGARWLPAKAKNSDFPGNWWIIETKHNVADVLAVIQQYA 10469 17236 - 2077 20882 315 2255 5.2675 251542.55 -33.5 0 restriction-modification (RM) CLAN071 APIS030 phrog_1685,phrog_37568,phrog_9133,phrog_29705,phrog_10818,phrog_21829,phrog_10089,phrog_62,phrog_11801,phrog_21007 APIS031 1 DarA restriction-modification (RM) Lida et al., 1987 https://www.sciencedirect.com/science/article/abs/pii/0042682287903242 YP_006494.1 https://www.ncbi.nlm.nih.gov/protein/YP_006494.1/ APIS031.hmm P1 of E. coli GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:3029954 https://pubmed.ncbi.nlm.nih.gov/3029954/ Bind to phage DNA, thereby masking type I R–M recognition sites and preventing degradation of phage DNA. PF18788.4,PF18789.4 MEQFNINKGMTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESNTLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSDKTGDVAQSGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNSDTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALVDEITQLQADSDQKEDAMSDLADQVAAVEGQKADLEQKINAVASEADSLYEENERLQGEIDRLNRTGGRDTIAPAGMTGGHSRALTDRLASIKNRMHMDGEATLSNGASMKQFIGDGEGYIQLTDPDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVEPESAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARYGIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATNSPDEFRVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEADAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQTAGRFEENEELVNGAARHLADLLVAIQKAGVAA 27904 29823 - 23527 32220 201 639 4.4870 69479.93 -28.0 0.39 restriction-modification (RM) CLAN040 APIS031 phrog_21969,phrog_1926,phrog_1639,phrog_26978,phrog_8883 APIS032 1 Cor superinfection exclusion Matsumoto et al., 1985;Uc-Mass et al., 2004 https://doi.org/10.1266/jjg.60.475;https://www.sciencedirect.com/science/article/pii/S0042682204005926 YP_007947949.1 https://www.ncbi.nlm.nih.gov/protein/YP_007947949.1/ APIS032.hmm Enterobacteria phage phi80 GCF_001015325.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:15518820 https://pubmed.ncbi.nlm.nih.gov/15518820/ Superinfection exclusion, inactivates FhuA receptor. MRKLIICMAGAVMLTGCAGVIEKQEPVCSGTAIVGGQETTVQIYGVRKQNNQTQYRAGYPFSWRWVSANTFTETTCK 178 77 8.8791 8479.81 4.0 0.57 superinfection exclusion CLAN072 APIS032 phrog_1119 APIS033 1 Lar restriction-modification (RM) King & Murray, 1995 https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1995.tb02438.x YP_009168126.1 https://www.ncbi.nlm.nih.gov/protein/YP_009168126.1/ APIS033.hmm Escherichia phage vB_EcoP_24B GCF_001308415.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:7476171 https://pubmed.ncbi.nlm.nih.gov/7476171/ Lar is functionally similar to RaI, is able to alleviate restriction and enhance modification by EcoKI. And the nucleotide sequences of their genes share 47% identity, indicating a common origin. PF14354.9 MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEARGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK 11 78 4.9995 8484.49 -1.0 0.55 restriction-modification (RM) CLAN041 APIS033 phrog_6422,phrog_599 APIS034 1 gnarl1 O-antigen-based barrier Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full YP_009615327.1 https://www.ncbi.nlm.nih.gov/protein/YP_009615327.1/ APIS034.hmm Klebsiella phage vB_KpnM_KpV79 GCF_002743875.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella; 570 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Klebsiella Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Putative O-antigen modifiers, interfere with O-antigen biosynthesis. MTEQDQRLKQFDEKLAELEKTIKQVQEQRREYINRKGLNK 35946 36068 - 32234 39551 54 40 9.8665 4963.65 2.0 0.53 O-antigen-based barrier CLAN073 APIS034 phrog_1402,phrog_13897,phrog_23045 APIS035 1 orf148 broad-spectrum counter-defense Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full YP_009790938.1 https://www.ncbi.nlm.nih.gov/protein/YP_009790938.1/ APIS035.hmm Escherichia phage OSYSP GCF_002627205.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Broad-spectrum counter-defense. MINAKEELLLALKNTNSEVKCIKIEFGYYGDKEVWVLPVGYTEKDIEDFLDNLDFKYDSGFGGQLLYGNVWFTDGTWLERGEYDGSEWWEYKTTPAIPEECRTINGEVDRTLLLN 6432 6779 - 4114 8818 111 115 4.0052 13375.95 -12.0 0.8 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972,phrog_16544 APIS036 1 gnarl3 O-antigen-based barrier Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full YP_009850475.1 https://www.ncbi.nlm.nih.gov/protein/YP_009850475.1/ APIS036.hmm Escherichia phage Mangalitsa GCF_008214915.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Silas et al., 2023 https://www.biorxiv.org/content/10.1101/2023.04.06.535777v1.full Putative O-antigen modifiers, interfere with O-antigen biosynthesis. MNYALYQYINRDGVVAHALVNTKTKDVMLADTVIYFERGNLVWKPAAHPEYVWLAIQNDKHHKIVAMATHPHFIKARG 4208 4444 + 211 5730 17 78 9.7411 9002.44 6.0 0.3 O-antigen-based barrier CLAN074 APIS036 APIS037 0 IMGVR_UViG_2541047688_000003|2541047688|2543982737 APIS037.hmm IMGVR_UViG_2541047688_000003 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PF03230.16 MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYTLAKKMMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD 142165 142590 + 138994 143247 3 141 4.7142 16245.46 -6.0 0.7 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS038 0 IMGVR_UViG_2576861805_000002|2576861805|2579667884 APIS038.hmm IMGVR_UViG_2576861805_000002 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Caballeronia;s__Caballeronia jiangsuensis 1458357 Bacteria Proteobacteria Gammaproteobacteria Burkholderiales Burkholderiaceae Caballeronia Caballeronia jiangsuensis PF03230.16 MILNNLDHNMAEIVATPVPMVERLTFLPKVFGLKLMMKAEALLYHTAEQLAAETYKGGYWEYMKVSNGCGYAAPVNPARMKVCVWGNGFEGEMSSDAAGIVFTLFVLNNLMFETSGKDEALTELLIKNWEQLRDYAATHAEARQIYRAID 22792 23244 - 18260 25532 5 150 4.9444 16845.49 -3.5 0.85 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS039 0 IMGVR_UViG_2751185776_000007|2751185776|2753238834 APIS039.hmm IMGVR_UViG_2751185776_000007 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Morganella;s__Morganella psychrotolerans_B Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Morganella Morganella psychrotolerans_B PF03230.16 MSNTTESAITMELVPDEQRLDFWFNHFGAVKGWATFEVVIFTTMGQFCDEYHGGYWEYGSLNNGGAFIYPDINADTLTLFNMHNGNEATVSQEAAGIAVCLILYSIWSFQTESEVMCDRFYQLRDYASQHPESSAIFHLID 10487 10912 - 6914 15002 49 141 3.9947 16050.82 -12.5 0.79 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS040 0 IMGVR_UViG_2778261704_000001|2778261704|2781089226 APIS040.hmm IMGVR_UViG_2778261704_000001 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PF14354.9 FVKSWCVGIGIVHRPVCRLMSCMSIHLPHDAIRVTVFFDGCSNSTMSFSATELSGVWILSEGVSMSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEARVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK 3768 4196 - 1 4196 8 142 6.6684 15541.78 0.5 0.42 restriction-modification (RM) CLAN041 APIS033 phrog_6422,phrog_599 APIS041 0 IMGVR_UViG_2832702071_000001|2832702071|2832704097 APIS041.hmm IMGVR_UViG_2832702071_000001 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_D;f__Amphibacillaceae;g__Paraliobacillus;s__Paraliobacillus ryukyuensis 200904 Bacteria Firmicutes Bacilli Bacillales_D Amphibacillaceae Paraliobacillus Paraliobacillus ryukyuensis PF00753.30 MIKITALGVGGAFTEKYYHNNYIFDFGERRMLVDAGTTIRYSLKESDYELNSITDILITHLHSDHVGGLEEIGQRCKFILNHKPNLWIREDQYREFRRSLNRGLETDGFTLLSYFNVCLFNIEEGFNIDKYKIVTIRTDHYHAKGMKSFGFKVIQPNNSSILFTGDIAKIYEKGLIWHIDDKTAMIFHDCSIVSNPVHASINEIRSYYGHELMERIYMMHYQDDTDVELMEKINNCNFVRQGEEYIL 923901 924644 - 920815 926868 4 247 6.2226 28993.97 -2.0 0.93 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS042 0 ivig_4422_51 APIS042.hmm ivig_4422 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli MKKIDIYSDTSAYVIGSLGFLIFFVWQYQSLSPGWRFLGMSLISLGAGIATQVLMYLFNGWLSKRVEKKRATSICRILAIPEDSTDQDDIAKCWRYMIARYSNELLANRLSDLIGIVVTSVGTIISIGISIWYVGMIVYFVWNRDFNEPSLLFIPLFFRVLAFICELLLSFFCNVLFNRYPGEARKFNKNYDELRRTDPFLSSKEFRDSIRN 31793 33622 + 27048 36410 16 212 9.0301 24608.75 5.0 0.69 restriction-modification (RM) CLAN039 APIS021 phrog_7745 APIS043 0 MH238467_00052 APIS043.hmm Pasteurella phage Pm86 MH238467 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Pasteurellaceae;g__Pasteurella; 745 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Pasteurellaceae Pasteurella PF13876.9,PF11195.11 MLKPNRQDPRMANRKRTKLILGETMENRVTKEHLESIIVDKKFHRLTETLTVCVLTLRNGFTVTGESACVDPASYNQEIGENIAYENAFEKLWQLEGYMLKTKLYDEKSAHYPEDIKNQIPFNFGYALNLLKKGKKVSRAGWNGKGMFLFIVKGGAITQAVAEHYGNSERPETNLPVLDAIYMKTADNKLVPWLASQTDVLAEDWNVID 26773 27402 + 24175 29790 7 209 7.8802 23827.33 3.0 0.53 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3934,phrog_872,phrog_322,phrog_34966,phrog_4435,phrog_9467,phrog_1048,phrog_16684 APIS044 0 HM144387_00160 APIS044.hmm Bacillus phage W.Ph. HM144387 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus; 1386 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus PF12706.10 MLKFIGRGSAFNTKEGTNAAYFVWDGELIIIDCGTEVFKRLKESGLLQQFTDIRVIVTHLHDDHAGSLSSLILYNYFCMGNQTEKNIIVYSPYDIKLKDFLDRTGCTTKYYRPRNFDNHITMDFTEPEDSIELSAVPQRHVEELLSYGYIFDFKGQSIYYSGDTNMLHEFVKKPSIINKFTRIYQDTCWLHYEGNVHLSLTKLVEAVPDRELRSRIYCMHLDEGMLSHEGTVRNLGFKVVEVDQVFK 109111 109854 + 108161 112981 9 247 6.1665 28728.72 -2.0 0.92 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392,phrog_14429 APIS045 0 IMGVR_UViG_3300005805_000580|3300005805|Ga0079957_100145420 APIS045.hmm PF11195.11 MEQSESSGPFGFGLLGLLHLGKGTFGEALEALKQGHRVARSGWNGKNMFLFLLPAGTVPTKAIHDPALRQVIEEQVGGETFEALGSIRMFTADKKILTGWLASQTDMLAEDWSIL* 5 115 5.6105 12473.33 -1.5 0.73 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_30130,phrog_872,phrog_1048 APIS046 0 IMGVR_UViG_3300009034_000088|3300009034|Ga0115863_100105265 APIS046.hmm MDKIKKFSIIKTRDKIYLGYMDYKSCYAKDLLELYFDGKKAKPSFHEAWVVVKKIPSKVEEEISQPDINFRYELKDKSFASDKIPLVIKRKDALSTDSKGYSSWKSEYADYRSLYEEKYDEQPNIKREIAFEVVNVIELEDVKKVNGFSYPASKTHKDSTTEITEKNVESQLLDKILFPNIVLPSRPSKLSSYQAYRIIRKHIQDNIDPKVAKITSDYDFCFSVCKKIKLVEPEEYKVNINYDPEGEAEYATRYRFDRPVKVFEMTYSPENYKGYTPIQGFEGKDHEDLKKNIDTYLKNLMAEINKPLKDCTHCKGTGVIID* 13 322 7.9840 37670.03 4.5 0.71 Gabija CLAN009 APIS005 phrog_20113 APIS047 0 IMGVR_UViG_3300009183_005911|3300009183|Ga0114974_10000105111 APIS047.hmm MKLSLSVSNTDNDKGEELFKQLRLIDMANAKQELLGMLERVGGVIKCATIRRGQHYWHDEETQNMLDRDLKEGYTPAEYEEFLDRLDFEYDNGYGGQELFGTIWLTKENTWLERGEYDGSEWWAYRVCPQILDTLKAD* 21 138 4.2837 16209.08 -11.0 0.66 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972,phrog_16544 APIS048 0 ON170191_00003 APIS048.hmm Ralstonia phage YO108_2 ON170191 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Ralstonia; 48736 Bacteria Proteobacteria Gammaproteobacteria Burkholderiales Burkholderiaceae Ralstonia MDNQHKHIKGYRDLSQTEIDLMNQIKAKGAELLQLQAQLVGHLSTALETKAHAARLSTTHEPWDQGASDECIELRRFKAAEPMRWAAIGKTDIETGVMALVRAVAQPAAI 84 110 6.9787 12156.91 1.5 0.75 cyclic oligonucleotide-based antiphage signaling system (CBASS) CLAN036 APIS004 phrog_29522,phrog_717 APIS049 0 IMGVR_UViG_3300009856_000087|3300009856|Ga0131695_10912748 APIS049.hmm d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;; 543 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae MNTPDISPEALRSVSGKMVACRAAEKLGITVARFYYLAQRHRVSTAYVRPLWSEKETQRLIAMRRGGMTVKRIAAELNRTEWAIKKKLGYLRKDGLI* 5 97 11.2003 11094.09 12.5 0.59 RecBCD CLAN037 APIS006 APIS050 0 IMGVR_UViG_3300010885_038456|3300010885|Ga0133913_1000088583 APIS050.hmm MINAKDELKSILPKQTLAENDGYTIKCAKIEKRDSKYWGDDYTETVYEHILPTGYSPQQWDEFWNGIDFEYNAGYGQQELFGNVWFYGPSWLERSEYDGSEWWVLRELPTIPNELL* 11 116 4.0862 13771.23 -10.5 0.79 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972 APIS051 0 IMGVR_UViG_3300012016_000701|3300012016|Ga0120387_100082722 APIS051.hmm PF07275.14 MNELSLFNDSISGAQSTDKEQSVKALSSSNNLNNDVVEVCDAELDLADFKYDFHTGNPAVYVSSYNRYNCGNLDGMWVDLTLCGDYDEFMQVCRQVVWDEQDPEFMFQDMENWPEAWYDEGSLSEDTFERIQEFAALDEDEQEAFEAFMDIRCDSEVSFEEFREAYCGKWDSEEEFTEQLVDDLGLLDEIPEHLRRFFDMEAYSEELFRYDYDFTDGYVFRVM* 6 223 3.5608 26277.46 -44.0 0.7 restriction-modification (RM) CLAN005 APIS003,ardu phrog_14321,phrog_2059 APIS052 0 IMGVR_UViG_3300012991_000489|3300012991|Ga0157148_10003866 APIS052.hmm PF11195.11 MDKYINASRVSAQHMTRGDYNKLRGWELPANENALDDGFLVVNHTVSECNVDGYDGYVSWLPALAFHQQYKPVGNGVSFGEAVEALEAGSTVARSGWNGKGMYLVLVSGKCVEQVINDCYGDLEDPTAFNPVLDAIYMKTADDKLVPWLASQTDVLSTDWEILD* 3 164 4.1386 18071.16 -11.5 0.6 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_30130,phrog_872,phrog_9467,phrog_1048 APIS053 0 IMGVR_UViG_3300014203_000236|3300014203|Ga0172378_100030802 APIS053.hmm VNGKKWTCEFDETGGYDCTSCAYIIKEDGFERLRIDNLQFRYKTAWDRNVPDVEMTALANKIVKLLNEEGGLKMEEQKNEYVLQAIVKSEDCVYVKGIKCDFYTNTINLSYFLIDDCVPVKTNEQRWFRVNSIPKSLKIKGSDKSTNYRFTLKNGITPSALLPEKIESEGSSIPEEYENVAGCYKMECDTIPGEWEERPFTLEIIYKSNDFEWLKTVYPHQHFLLDQIECPSDLLDSIKPCIADRKQMYTFIRDYIKKNINPKVASITSDYDFVFTVARDVKLYEPIHTERNAGTSKRPKWVNDIKDKKTIMIYSITTEASYLKGGCHYPDPVIGKNAKDLDEKINKTLEEIMKEINKEFCECPTCKGWGYVEVK* 4 375 5.2783 43503.68 -5.0 0.54 Gabija CLAN009 APIS005 phrog_20113 APIS054 0 IMGVR_UViG_3300014204_000482|3300014204|Ga0172381_1000105623 APIS054.hmm PF00753.30 MELYVLGSGNAFTKKNWQSNFLIHQNGKWLLIDCGSFASIALKEEMGLNVWDLDAVYVSHIHADHVNGLEEVEYCTYFNPAIPRPKLFVQGQYIINNEGQAFSSGLVNDLWHDCLKAGSRGLEKLDAQLHTYWDVQAVESNGHFIWEGIKFDIVQTVHVSAHRKIENSFGLMWNDPDTGERVYITTDTQHCPVNAMMAYLTECDVIFHDCETAPFASNVHAHYESLKTLPAEIKSKIWLYHYQDNVIDEWDMWSTKAQKDGFRGFVKTGAIFGRTYSEQEAGCIGKSYYTKMARLEAENAEMRKKLAAYENKKGSQD* 10 317 5.6870 36215.78 -6.0 0.81 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS055 0 OL763419_00042 APIS055.hmm Pseudomonas phage Churro OL763419 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas; 286 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas PF03230.16 MSKITATRIDNDDQRLQVLPAHFGADFLRVEMALYDHLQKMAPEDYNGGYWFMYQLSNGAMYLAPAITDRKLRLTVDTNGYSGEVSGDAAGLITCLFVFNALCWKYPQREDFVDLFYKLRDFAFDHPEAEEIIAAID 20 137 4.3810 15688.77 -7.5 0.88 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS056 0 IMGVR_UViG_3300020049_002891|3300020049|Ga0206652_100507913 APIS056.hmm MQHQREINKLLGLISDETLEILQESNAIIAGGALTSIFCNNEVNDIDVYFKSEGDFMLFINLVFSGGHYLICNNYTDRSILFKDKDTGQDVQAIVYKFFPDADAIFADYDYTINMAAYDCATEELHLHEDFLKHNSQRYLQFNTGTAYPLISALRVNKYVDRGYTISKPQYLRIMMTIAQLKLESWADVRDHVAGMYGLDLTQVFPDDAEFSIDKAIEILDGIEPDAKIWSPRTDIDEDEIISKYFDGRYKDSRESCEGLYFKNVMLKPDGTICSHFKPEFEYVIGGTVDGGSNGIYFAEGYGVLSACYHDSDNNVILQIEGEKPTKRWESSFKGEVKVVAKYTRDEFYRKFKKTNSNLSLFKH 6 364 4.6222 41704.94 -15.5 0.75 Gabija CLAN023 APIS008 phrog_18122,phrog_27100,phrog_4400,phrog_25514,phrog_37109,phrog_16176 APIS057 0 IMGVR_UViG_3300021167_000128|3300021167|Ga0216365_13592277 APIS057.hmm MLKIKYYSTNVGVIFKIAHSYVYDLNSYCTYIDDTYVDNIQELNNRWYKIDNIPFSIKKKVPTKKTLVGYELKSTSLASDNIPAFLTLEQLTPVEDDDDIYGYSGEYKEFAYLYKPKFDDIPEQLEEVPFELIDLGEIEVENYNSPEKIIIKQKHERTDGKPTNVDLSSIVRYEELHEILTPEFLVHNCPCTLSSEQMFKIIRYYVKENINPSEARITSDYDFCFTVKKVIKKEPSAVTKYKKSTEDLFEVFEMTWSGYGGKSTGYQGYTPIQALNASSLGDMKVKLEEYLTKLMDIINSEVEQCDCCKGTGHVVNKIETNFKL 3 324 4.7050 37511.63 -11.5 0.74 Gabija CLAN009 APIS005 phrog_20113 APIS058 0 IMGVR_UViG_3300022562_000702|3300022562|Ga0247387_1000200120 APIS058.hmm PF00753.30 MKLQFAGVGSAFTTQDYYQSNAVIRPDTGKVFLIDCGSDARFSLGEIGLSHRDIDAVYISHLHADHIGGLEWLGFCTYFDPQASKPKLFIVDSLVEPLWDSLKGGMQSHEGIVLTLDSFFDVTTVQINNCFTWKSVRFCPIQTVHVMNGLGIVPSYGLIINDKTFFTTDTQFCPRQIEKFYDMADIVFHDCETYPFKSGVHAHYDDLKTLPDKHKEKMWLYHYGPNPPQNPTEDGFQGFVVRGQEFELK 53 249 5.4744 28310.10 -5.5 0.87 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS059 0 KF302034_00153 APIS059.hmm Pseudoalteromonas phage HM1 KF302034 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Alteromonadaceae;g__Pseudoalteromonas; 53246 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Alteromonadaceae Pseudoalteromonas MSEQVLNEIQEVITRSNKGKYSRQKKQLLSLVNETEGLEELFKELGCFVAGGAVTSVFTNKEVNDLDIYFRDQESLIKFIKVAFGETPCVPCTPVSVAPLSPPSPVNFNQDVTPSSVELDIFALRYVGHTDKSVMFLDGSGMQVQAIHCGFYRTVEDIFKSFDFTINMGVYDFAQECFVLDEDFLTDNASRKLRVNEGTSFPIISQLRLAKYQQRGYSINRKEFIKLCLSVASLNLSSWEDVKNAIGGMYGYNMDDLFDEDKDFTMEEVFEQLEELECNLEGTTAKIPATDLEDLLEFIEEQHEPNDPDKEYFYYKKVLKTSEEGVYTSIYKRDFKYIVDESPDNSGNGIYLYKSVDKAKNHYGLGSNDGAVVIKLTSGKTRAKLQSDGGGKYSTCTKLTVVGEMDLEDI 89110 90342 + 86250 92244 4 410 4.3665 46301.13 -23.0 0.69 Gabija CLAN023 APIS008 phrog_18122,phrog_27100,phrog_4400,phrog_25514,phrog_37109,phrog_16176 APIS060 0 IMGVR_UViG_3300024258_000005|3300024258|Ga0233440_1000037199 APIS060.hmm PF00753.30 MKIKFLGSGSAFVNHEENFQSNILISEGTSNLLYDCGTTINDALLFNSVSLQDITDIYISHLHADHAGGVEFVAFKTYFDGFPFGQHKPRMIAHDDIIKNGWECTWKGGLGAIHGQKMELSNYFDITSHTDNGIFFFEGIEFSPELSTHVNNGENDVPSFGLSWEYNEHSVFISGDCKLPESFTQYETSTIIFHDCEFAEYLGSVHTQFHELKELPDEVKAKMWLYHYALNDKHIWEYETLAEEAGFAGIVRRGQEFNLGLIE 3 263 4.5051 29902.25 -17.5 0.9 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS061 0 IMGVR_UViG_3300024337_000617|3300024337|Ga0255060_100056963 APIS061.hmm MATKKVAPKTETKTAPKKEVKSAAPKKEGKTAPKKEVKVAPKKEVKSEIPTTSAPKIMAKMVPAKKTAPKQDQDYVVLEHDLLTKNYTMIIHEANFKFNAPHHFKVIAAQPDTDGEHKGQNRVVGIVNFQEGPIKENGVNGVANEDLLGMVLCRLEGFQNSEYKCRENALAITKIEEALMWLRKRTNARVKRGVEGTSKV 9 200 10.4089 22213.85 16.5 0.55 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_22140,phrog_23843,phrog_2428 APIS062 0 IMGVR_UViG_3300028374_000006|3300028374|Ga0306906_100003345 APIS062.hmm PF00753.30 MKFTFLGTGGAFSRSHENFHNNVLIQTDSGKKLLIDCGGTALESLDELGVDPLDIDGVIVTHVHADHVGGLEELGFRGLFLGPKQKFDMYVPNLLVPSRAVVGYDSDVYPDLWHNCLKGGMVHIQDAEGNAVQADMDTYFNVHVSESPKDPIKVGGIKFIFVKTNHVPNKTSYGLVLESETGTTVFFSADSTSERTVDLEAFDIVFHDCMFMPRYPATVHTHFEEMVDLPESVRKRTYLMHYGNPDNAPEDLQGMRLARKHQTFEL 6 266 5.1357 29693.55 -9.0 0.89 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392,phrog_14556 APIS063 0 IMGVR_UViG_3300031923_000006|3300031923|Ga0326331_1000015821 APIS063.hmm PF07275.14 MTMTATTTARVYVGTYAKYNSGSIEGAWLDLEEYSDKDTFIAACEELHKDEADPELMFQDWEGIPEGMISESHIDSEVFAWLDLDDDEREILAAYRDNIDQTGDIDQAREAYSGRADSKEDFAEDLYRDCYTIPKELDCYIDWSRVARDLECGDYTFVYAGGEYIVFRNV 57 170 3.7939 19553.30 -27.0 0.56 restriction-modification (RM) CLAN005 APIS003,ardu phrog_2059 APIS064 0 IMGVR_UViG_3300033145_003233|3300033145|Ga0366840_1000004654 APIS064.hmm PF12706.10 VSKLTFLGVSGALSEGFNSNMLIDIPATEAKRDKYTLLIDCGEDISQSLKNANRKVEEIDGVYISHLHYDHMGGLSWLAYYNYFVLKRKIYLYIHESLVDSLWQMLSPAMNRQHKGTRFETLETYFNLHIVYDRHLDFWFTTMECNLVKNLHVDSFVGDMYSYGLSIIYHHRKGLDKYKKRLYISSDTVSCPPDEIWDEEIDLVFQDADTLNSGGIHCNYDILKRMPSDRKKDIWLYHYTDLSKFDEEGSKRYGVMPDAVADGFAGFVKEGQIFEF 4 276 5.7244 32163.46 -5.0 0.85 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS065 0 KJ018209_00009 APIS065.hmm Shewanella sp. phage 1/4 KJ018209 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella; 22 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Shewanellaceae Shewanella MTTNKLSIVAYNTDKGLYYANNQRDYNKQRVSIYSGILVNGGQPVDSEHKSGWYFLKGEDKITSVKKNQSGGYGEASWKLLDDSTHIDGVIPKVLTPTEADEFEDDCEWYIGQGSKYYAYRGLYKRVQERLPNIEVDVEFEVDYKGHIEYALVENNYKDMKIRVSNGSSWTPKVMEQSLTSITHYYELEELLTPDIVLHNRPCYITQDTTYNIVRNYIKENINPKHARLTSDYDFCLTVKKVIHIKPFTKSTEITKSNGRSYAKPQFKTQTISTKEVEIFEMCPSKKYQNYTPIEGFKGNSLADLVENMKLYLDHLMGVINTPVEECTSCGGLGCTYNKIDDINIRGE 4179 5225 - 1802 7010 11 348 6.1114 39989.06 -2.0 0.71 Gabija CLAN009 APIS005 phrog_20113 APIS066 0 IMGVR_UViG_3300035396_000446|3300035396|Ga0393109_006065_59543_60412 APIS066.hmm IMGVR_UViG_3300035396_000446 PF00753.30,PF12706.10 MRITIIGCGNAFSKRNFNQSFVLEQDGKRMLIDCGRLVPEALTNAGLSFKDIDSVYISHQHNDHCGSLENFAFDRYDWMNRPVRWNDFTQLENIGKMKPYAPKLYAQKELIKSLWKNTLRGGLESMEGFDATIETYFEPKPVDKSFIWQGWNFELIQQVHIMTGSKISDTFGLIVSKQGHQSIYFTTDSQHCSPKQVRVFYSKADIIIQDCECLPFKSGVHANYTELAGYPEANAEVLPLEIKNKMWLSHYQDYVLDDKDFFGKPCDWKTKAKDDGFRGFLEVGQTFDI 59543 60412 - 56393 62836 12 289 6.2977 33367.89 -1.0 0.89 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS067 0 IMGVR_UViG_3300035506_000204|3300035506|Ga0376461_0000200_6069_6716 APIS067.hmm IMGVR_UViG_3300035506_000204 PF07275.14 MSNLLNSTKMKFQFGEMNITPSVVNRLHELDYTVPELEDAIQEHVSHLDDEPSVYCGTYAKYNEGSLRGLWIDVSSFDDYHEFINFCKAIHADESDPELMFQDYQGFPRDFYSESCMSESDFYKILDYKNMCDLHGAEAIDDYIDLGHDLADFEEAFCGEWDSEEDFARHIVDECYDIDKSMGSLARYFDYEAFARDLFMYDYSMGANGNVFRFI 6069 6716 + 10713 10571 15 215 3.9397 25113.60 -29.0 0.82 restriction-modification (RM) CLAN005 APIS003,ardu phrog_2059 APIS068 0 IMGVR_UViG_3300035698_004593|3300035698|Ga0374944_618332_7825_8844 APIS068.hmm IMGVR_UViG_3300035698_004593 MIKIIGIKCKGSFYVTQFDQRSNYTEYSSVKHLLFDGNIPLLTFHKKWCYVGKEPTLIQKTVSQPKINHRYELLDNTMESERTPLVFEREAVAQYDDDESDWYWKQEFRMYKSLYKLVSDSQPDVLEAVEFEYETILEAERMPLEVAPFAHKIGFGDSYDRKTRALCDDDVHHQLLDQILFPQIMLPLRPSKLTSAHSYEVVRQYVKKHLDGRYAEITSDYDFCFTVKKTIPLSETEYFTVDLNAWHNVFSKRKRRPKLEKRFRKSREQICFEMAPKPYQSYPVIQGFRGDNHEDLKANMDRYCEELIAFINTPVVDCKHCKGAGVVLDAMYPLPDVGE 7825 8844 + 10147 10061 5 339 6.5697 39942.60 0.5 0.67 Gabija CLAN009 APIS005 phrog_20113 APIS069 0 IMGVR_UViG_3300036792_000137|3300036792|Ga0376663_0000427_343_774 APIS069.hmm IMGVR_UViG_3300036792_000137 PF03230.16 MNTFTHAAPVAENRRLNFLPKMFGTNYFLNGEASLFDFAHALMPDYAGGHWEFFHRTGDGVHFAVPSMPDASRLCVSGNYFDREVSAQAAGIILSLFALNAMAHRASSRGDETGTDFLADRYYALRDYAKDHPDRAAIFAAID 343 774 + 1288 1029 5 143 6.1247 15871.73 -1.5 0.77 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_10132 APIS070 0 IMGVR_UViG_3300037297_000240|3300037297|Ga0394146_0000041_21955_22497 APIS070.hmm IMGVR_UViG_3300037297_000240 MKYYIGTKAVKAEKMSLYDFQEEKFGKVDKSVENQLGYKVEYKGGYVSWSPKKPFEEAYHEVDVDGTVEKGTQEHTFIGKDTIKFNAPHNYTIKGMNIDADIILGHIHFQEGPVKEHGVNGIFHEDLINIVIDRLEHFQKSKFSCRENAVAITKLEEALMWLRKRTDDRKMRGVQGTSKV 21955 22497 - 19535 24471 8 180 7.5494 20758.65 4.0 0.58 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_22140,phrog_872,phrog_32732,phrog_23843,phrog_2428,phrog_9467 APIS071 0 IMGVR_UViG_3300037628_000643|3300037628|Ga0310922_0000037_90720_91751 APIS071.hmm IMGVR_UViG_3300037628_000643 MTDAYKLKAYTSKEGVFVAEVQKYSYGYYPIKFDVYRINDKDPKLTASGYWFFFPDKDIFSVKRKVNPKNINHRYILKDDSMKSKKIPLKLSREDVGYSQDSDYDTYWKNFSSLRSLYKEIYDTTEGSWEEIDFTVDVIGDIEISDLSKPESMVVSVLNDESWTHQGVKEIDIMDVAIFSELEQMLVPEFAIHKRPCSISSKITYSIVRQYVKDNLNSSVALITSDYKFCFTVKKKVKIKPYELRSEQKKSNGRSYAKPRISSKNVDHKQIEVFAMTHAGENYKGYIPIKGFKGESINDLIENVKLYLDELMQHINSDVSECEHCSGYGHVMTGSHSLNGERV 90720 91751 + 86259 93331 8 343 7.1914 39700.05 3.5 0.7 Gabija CLAN009 APIS005 phrog_20113 APIS072 0 IMGVR_UViG_3300039331_000150|3300039331|Ga0169804_01911_10006_10671 APIS072.hmm IMGVR_UViG_3300039331_000150 d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;; 815 Bacteria Bacteroidota Bacteroidia Bacteroidales Bacteroidaceae PF07275.14 MKRKRPPRPETHKKKSDLRETGKKVMKLNSLTSQIRINFKRHRIMEAVTLSEARVYVGTYNKYNNGSLFGKWLDLSDYSDKDEFLEACRELHKDDQDPEFMFQDYENIPEALISESWLSDKFFELRDAIEKLNETEQEAFFVWCDHHNSDISEEDADDLISSFEDEYQGEYKDEEDYAYEIVEECYDLPEFAKTYFDYSAFARDLFITDYWMDNGFVFRCA 10006 10671 + 10006 9660 14 221 4.3157 26491.29 -19.5 0.58 restriction-modification (RM) CLAN005 APIS003,ardu phrog_2059 APIS073 0 IMGVR_UViG_3300042197_004918|3300042197|Ga0451494_0000046_37885_38742 APIS073.hmm IMGVR_UViG_3300042197_004918 PF00753.30 MKTIQLEDSAKVPTLDQIKKHSEDGLVLCFAGVGNAWARKNANTSLIVAKYGKTILVDLGTSVPVSLESRGVSMLDFDYYHFTHSHADHVGAVEELLLKLRYIKKKKAKVIITHEYQQTLWNETLKGGCEINEDGLLRFTDLIDVVRPNWESCQPREKYHIKLDGILDFEIFRTFHVPGDVNSWEKAFWSTGLQLDGKVVFTADTRFDLSIFEHLDFSSVEAIFHDCQLSGPGSVHATYEELSRLLPSYKERMYLTHYGDNFDKFSPEKDEFAGFAKPWVLYKFD 37885 38742 + 33450 41191 14 285 6.0793 32666.12 -3.0 0.89 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 APIS074 0 IMGVR_UViG_3300042691_005779|3300042691|Ga0415385_0000706_88760_89182 APIS074.hmm IMGVR_UViG_3300042691_005779 MRNAKQELLNAMGSSITDLRCATITYNPFYDERPKKMVLKEGYTQADLDEFISKLDFEYDAGYGSQELFGMVWFNDGAWMDRYEYDGSECWDWHKYPSIPDELKEGALPEPDTDYPDDYDDMINQMINQEYDENNQPEHD 88760 89182 + 86250 91205 4 140 3.8160 16545.03 -21.0 0.63 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972 APIS075 0 KY083726_00051 APIS075.hmm Pectobacterium phage A38 KY083726 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Pectobacterium; 122277 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Pectobacterium MSKLNARKEFEDHVREVSSVKCAILSWGDPNCSWRDEDVERVLLYPGFTDQDYNEFLRRINTNYDSGFGGQELYGTIWYTDGTWSDRREYDGSEWWEHQSCPTYPEEFNHACITVSPDSPKLLG 46194 46568 - 44598 48325 103 124 4.2934 14542.86 -9.5 0.69 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972 APIS076 0 IMGVR_UViG_3300044693_000096|3300044693|Ga0466961_0000159_42271_43065 APIS076.hmm IMGVR_UViG_3300044693_000096 PF00753.30 MANITVQFLGTGDAFSKKYGNTSALVFVENKGVMKKLLIDCGRTTPDDLFSLGYTWSDIDAIFITHLHGDHVGGLEEAGFFSRYILNRNQHLIFPHVKIKNDLWDKVLKGTMMNGDLDRLMTFEDYFTYEAVGPEEQFFFFNDVMFSVFPTYHIKNKKSYGVIIGEKDFIIYSGDSLLNQDLIAIGFADDCQAVFHDCALYQKKDMVHASLQDLLTLEPEWRENIYIMHYGDNLSDKYLEIKDAGLKIAVRGEKYHFEVGEYCP 42271 43065 + 40061 45579 10 264 4.9035 30317.55 -9.0 0.9 pyrimidine cyclase system for antiphage resistance (Pycsar) CLAN008 APIS001 phrog_392 APIS077 0 KU946962_00005 APIS077.hmm Proteus phage PM 116 KU946962 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Proteus; 210425 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Proteus PF08684.13 MTTYNEVLDSAIESLKEYIECNDIQEETYEIQDAISEIADNAIPIYNCDIFSVFAEGGISYQMDDTGLIEGCDDVIQILQMRIYEELSNDLYHMVRGLINEYVYSLEEE 2028 2357 + 619 7217 81 109 3.3526 12584.91 -22.5 0.79 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_2568 APIS078 0 KX078568_00026 APIS078.hmm Morganella phage vB_MmoP_MP2 KX078568 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Morganella; 108061 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Morganella PF17574.5 MNQRNTIRLSDTVDSYERKVHINVRNGKVTMVYRWKDRYSDKKHTQRTTMNDDQTEHLLSVLERMREKIAEESEEV 14296 14526 + 11039 17803 3 76 8.5338 9240.35 2.5 0.62 toxin-antitoxin (TA) CLAN024 APIS011 phrog_2254 APIS079 0 MF042361_00008 APIS079.hmm Pseudomonas phage Skulduggery MF042361 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas; 286 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas PF11195.11 MKTFIGTKLLYAAAMSLGAYNHLQGWTLPADQDPEAPGYLVEYTDGGKPNHKDYEGYISWSPKDVFEGTYRPVDGMTFGLALELVKKGGHIKRKGWNGEGQFVYLLKGSSVASGLGYGFGEYLGEPTFTDLLILRNTHNQLASWVPSIGDLLASDWQSVEVEGV 4858 5352 + 1415 10494 47 164 4.9286 17982.27 -4.0 0.55 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2709,phrog_30130,phrog_872,phrog_32732,phrog_9467,phrog_1048 APIS080 0 ON602753_00020 APIS080.hmm Klebsiella phage VLCpiA3d ON602753 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella; 570 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Klebsiella PF17574.5 MTTIKAKFPGNTIQLSDTVDQWGRKVHINVRNDKVTLVYRWKAKSDNRAHTQRVTLDDVQAARLLASVAVAATIAVGEDKVREAILSKEVGSTATRLAEASEA 39 103 10.3987 11273.83 5.0 0.73 toxin-antitoxin (TA) CLAN024 APIS011 phrog_2254 APIS081 0 MGV-GENOME-0326027_55 APIS081.hmm MGV-GENOME-0326027 PF18788.4,PF18789.4 MTVTLTDKILLKDVPGYIAPAVSDDIYNELMKGQSADLMLESATIEEVDQFYLGDELVYVSHAAMFEAISTERMRLSQTMRAFVRALNRGLNGTDIKAGTDDAGLDDSGEKTIGGAVIGKVRRVNSIPIMTALIPLSDGQSISLVFHSPTADNGRVKNNDLLVAFRFLLNKRDVTHIVAPIGGRDVSLQQVTQALANLAERNSAKFTKQQDAQVKLRGDIDALNAENDQLSEQQSSLLTQVESLQTKLIAHQSDERDVREKLANQRRINAELEAQIVALQGVSQAGATDTGSSFTDATRKVKDRMGIDGKATLSNGAIIRYNSYDHDGELQGSVIITDPSGKVYEMPSPSSQGGAMGVTATKLLKAYRENRADRYVVTTPPPELPPEQPPEQPPELPPEQPPEQPPEQPPEPTAKYRYALTSRPASIGAVPADHTAILDVPEQSDKYGRLARHGFIEYARKLTEQEVSNFELKLIPTLADLDVLAKTVVSDSMANYAQQYVEMAESDPATFGSQVKLNTKKAAPNIAYPEGDDLDYFMEKVKAELVSQTPPTPPQTNEETPVVSEADTAANDALSYLDAVMQLQSKDIAEIRDARSKVRGAIAALQNAGRFDENEDKVNAAAQHLSDLLVAIQREGAGA* 27 639 4.5635 69478.91 -24.0 0.38 restriction-modification (RM) CLAN040 APIS031 phrog_21969,phrog_1926,phrog_26978 APIS082 0 MGV-GENOME-0365202_107 APIS082.hmm MGV-GENOME-0365202 MINAKEELLEALQRSGKSIEDIEFAGVYYDCSNYDIGESGARCATEDTDFLLDFLNFSYDEGFGMQYIYGYILFSDGSWLERREYDGAEWWKFMTLPTKESIEDYINSRESSRDII* 8 116 3.8595 13559.92 -15.0 0.77 broad-spectrum counter-defense CLAN018 APIS035 phrog_3972 APIS083 0 CP067347_00056 APIS083.hmm Clostridioides phage ES-S-0107-01 CP067347 d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Peptostreptococcales;f__Peptostreptococcaceae;g__Clostridioides; 1870884 Bacteria Firmicutes_A Clostridia Peptostreptococcales Peptostreptococcaceae Clostridioides MMNIYMVKTNKECFISDCEVFQGYGYDYHRTKLTNIYFDGEKPKETYCKNWYVIDKYPERIETKLIEYTNKRYELNNKNMECEKFPKTINVEDIDKFDFEIREFYTFQKDELPPKLVEVENVKIEVIMELDSFEMPNKIEYEANGVKENYKNSSVYKITNADVKHQLLDKIIFPELMLSSRPCKLTSKQMFDITRQYIKENIDLSKAKITSDYDFCFTVKKIVPLLTPEKITYQNVFAKTKKERNKINFKIKEYKEVKIFSMTHNQCNYDGYTAIDAMYANNENELKEKVDTWLKGVVDAINKPLESCPHCNGTGYIDDIKK 35664 36632 - 34332 39330 47 322 6.4978 38315.99 0.0 0.69 Gabija CLAN009 APIS005 phrog_20113 APIS084 0 MW001769_00023 APIS084.hmm Pectobacterium phage phiPccP-1 MW001769 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Pectobacterium; 122277 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Pectobacterium PF17574.5 MSKIAPKRLTQTIQLSDTVDQWSRRVHINVRGGKTTLVHRWKSRKDGRDHTQRVTLNEHQAGRIVGALTLAVAKIAEAKGKHIEDNFCAAVGRQVEDTAPNFE 14218 14529 + 11092 18133 3 103 10.9590 11566.16 9.5 0.58 toxin-antitoxin (TA) CLAN024 APIS011 phrog_2254 APIS085 0 OL964750_00001 APIS085.hmm Escherichia phage T7 OL964750 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PF08684.13 MAMSNMTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHMAADNAVPHYYADIFSVMASEGIDLEFEDSGLMPDTKDVIRILQARIYEQLTIDLWEDAEDLLNEYLEEVEEVARQKEIDRAKARKERHEGRLEARRFKRRNRKARKAHKAKRERMLAAWRWAERQERRNHEVAVDVLGRTNNAMLRVNMFSGDFKALEERIALHWRNADRMAIANGLTLNIDKQLDAMLMG 5 231 5.8885 27280.88 -3.0 0.51 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_2568,phrog_2828 APIS086 0 ON229909_00228 APIS086.hmm Escherichia phage vB_EcoM_CE1 ON229909 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia MHIVLFKPTPYNVRKNTQFKALIADTWELVLDIPAEESPPFGRVEFIKFAVRPTKRQIRQCKRYFRKIVKLEK 14 73 10.9149 8775.46 9.5 0.58 toxin-antitoxin (TA) CLAN038 APIS012 phrog_8921,phrog_2375,phrog_3272,phrog_12748 APIS087 0 ON528733_00011 APIS087.hmm Shigella phage ESh23 ON528733 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Shigella; 620 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Shigella PF08684.13 MERNTNAYYDLLAATVELFNERIQQDEIAEGDDYSDALHEVVDGQVPHYYSEIFTVMAADGIDHEFEDSGLMPDTKDVTRILQARIYEALYNDVYNSSDVVWFEAEESDEEDEYWVVDAKTGAFIEQAVSLEVATACAKDHYAIGRRLKVEDINDNVVFDPAAAEDCE 3 168 3.7150 19066.68 -30.0 0.58 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_2568 APIS088 0 uvig_218805_40 APIS088.hmm uvig_218805 MNYIYISKGDKWSCDLSNYLFDGVKAESTNKKEWYRLDKIPEIVSEKQADKRINERYELKAGYTATDLMPKIILKEQIDEYEEVIGLYTYRYDSVPGGYDEIEFNIEEIYERKDFVFVPNKYSAETDLITQIEYPEVAYQDKPCRIDSEHMLKIIREYVKRNIDTSVASIKSDYDFHFEVAKKISLADPYSIQVDTNNSIIDKRRKPKWVDRLISTKEATIINFKDKPSSSNYGKDCVIAPSITGENYTDLQNKVDKYLSELIQQINKKYCECPTCKGWGIVEEE 31655 32539 - 29247 36568 41 285 4.7576 33416.75 -9.0 0.69 Gabija CLAN009 APIS005 phrog_20113 APIS089 0 uvig_330502_68 APIS089.hmm uvig_330502 MYDWQKRKLKRELGDLYDTFLDYECYLAGGAITSIFTNKDINDYDIFFKHKDDIVRFLHKESYGTNFKSDYAITIAKNGKTIQLIYKHEYPTAQDVLNSFDFSVCMGCYDFSTEEFVLGDTFLEDLASNTIHFNPKTDSPIMSLIRVKKYMNKGFDIPVPEMFKLGFTISQQNVDSYDKFQSLLGGMYGENYNKFAEKLKNEGKPFDLNYVVSKLSEIDDTDKSCGCTTELDFSEMNESEISMEVGIPFDYFEYDDEYYTLDFKRLDKEDVDKCPSARKKPIEEIIKLPMTVYKWIDAKNRCSFFNSNFKWKDYGEVKTNSWFASDHGLHFVTEDKIWTCCYSNNSNKALVRGVVTSYDDFISVDDMNVRNQEMKLERVFVTNIWNEEDIPDEIKNKKKDKQEDYPF 29840 30154 + 27418 32841 29 407 4.5769 47822.78 -18.0 0.7 Gabija CLAN023 APIS008 phrog_18122,phrog_27100,phrog_4400,phrog_37109,phrog_16176 APIS090 1 IpI restriction-modification (RM) Bair & Black, 2007; Rifat et al., 2008 https://www.sciencedirect.com/science/article/abs/pii/S0022283606015919;https://www.sciencedirect.com/science/article/abs/pii/S0022283607014234 NP_049749.1 https://www.ncbi.nlm.nih.gov/protein/NP_049749.1 APIS090.hmm T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:17188297;18037438 https://pubmed.ncbi.nlm.nih.gov/17188297;https://pubmed.ncbi.nlm.nih.gov/18037438 2JUB Restriction endonuclease inhibitor IpI blocks the ancestral two-component type IV R-M system and represents a third generation bacteriophage defense against restriction nucleases of the Gmr type. PF11634.11 MKTFKEFTSTTTPVSTITEATLTSEVIKANKGREGKPMISLVDGEEIKGTVYLGDGWSAKKDGATIVISPAEETALFKAKHISAAHLKIIAKNLL 73591 73878 - 70360 76885 38 95 9.6274 10176.80 3.0 0.52 restriction-modification (RM) CLAN075 APIS090 phrog_4363 APIS091 1 HOS17_gp01 restriction-modification (RM);bacteriophage exclusion (BREX) Andriianov et al., 2023 https://www.biorxiv.org/content/10.1101/2023.02.27.530186v1.full YP_009792928.1 https://www.ncbi.nlm.nih.gov/protein/YP_009792928.1 APIS091.hmm T3 of E. coli GCF_002745435.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Andriianov et al., 2023 https://www.biorxiv.org/content/10.1101/2023.02.27.530186v1.full T3 encodes a SAMase that circumvents the SAM-dependent Type I R-M defence of the host E. coli through cleavage of S-adenosyl-methionine (SAM). SAMase also allows T3 to evade BREX defence. MIFTKEPANVFYVLVSAFRSNLCDEVNMSRHRHMVSTLRAAPGLYGSVESTDLTGCYREAISSAPTEEKTVRVRCKDKAQALNVARLACNEWEQDCVLVYKSQTHTAGLVYAKGIDGYKAERLPGSFQEVPKGAPLQGCFTIDEFGRRWQVQ 900 1358 + 900 5988 203 152 7.9480 17012.38 3.5 0.78 restriction-modification (RM);bacteriophage exclusion (BREX) CLAN042 APIS091 phrog_2226 APIS092 1 Gam RecBCD Bobay et al., 2013 https://doi.org/10.1371/journal.pgen.1003825 NP_040618.1 https://www.ncbi.nlm.nih.gov/protein/NP_040618.1 APIS092.hmm Lambda of E. coli GCF_000840245.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:24086157 https://pubmed.ncbi.nlm.nih.gov/24086157 2UV1 The protein Gam inhibits the host RecBCD exonuclease activity thus allowing efficient rolling-circle replication. PF06064.14 MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV 32816 33232 - 30839 34357 14 138 5.7699 16346.28 -3.5 0.52 RecBCD CLAN076 APIS092 phrog_259,phrog_26899 APIS093 0 MN450150_00001 APIS093.hmm Pantoea phage vB_PagP-SK1 MN450150 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacteralesl;f__Erwiniaceae;g__Pantoea; 53335 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Erwiniaceae Pantoea MIHTKEPANQFYVYLTAYRALNGDAVNEKLLKGMISEIRKYPGLYGVIENEMVAGCFREHGQEEASIERTLKVRARNETEADNLAFLACDRYDQDAVLEVASQTHTATLVSYKREGVHGTLNRSREVIGVYTAVESAQGECYSVEGGAQVWEVL 937 1401 + 937 6659 11 154 4.8782 17223.32 -5.0 0.82 restriction-modification (RM);bacteriophage exclusion (BREX) CLAN042 APIS091 phrog_2226 APIS094 1 Rad Retron Azam et al., 2023 https://doi.org/10.1101/2023.03.15.532788 YP_009829723.1 https://www.ncbi.nlm.nih.gov/protein/YP_009829723.1 APIS094.hmm Escherichia phage SP15 GCF_004768945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Azam et al., 2023 https://doi.org/10.1101/2023.03.15.532788 Rad inhibited retron function by degrading noncoding RNA, the precursor of msDNA. PF13155.11,PF13155.11 MLNSLYNISINLKEEPNMNVDELTQHLLSRGFDTDKYHCWLSPEGWLTVPLYDFSGMLRGYQTYNPSAPKGHGKCPFEAKYFTYSTTQCVWGLETLNGDEKVVLIAESVFKAVALHNAGYPALAMLGSSPGKALLKQLKLLPFKLVAVGDNDPAGEKFARKLNGFVSPVDVDEMSTENLKNFLAMKLNF 35930 36499 - 33935 40979 95 189 6.4794 21059.21 0.0 0.85 Retron CLAN025 APIS094 phrog_849,phrog_849,phrog_14043,phrog_14043,phrog_17952,phrog_17952 APIS095 0 IMGVR_UViG_3300014656_000059|3300014656|Ga0180007_100010693 APIS095.hmm IMGVR_UViG_3300014656_000059 ;;;;;; PF09588.15 MRFDLEQGSQEWLDWRRMKITGTDAAVILGLNPWKDVKQLYMEKVDGVLPYVNQRMRDGNRLEPEARAEYQKMTGNGMMPAVVQHDENEWMGASLDGINFYGDLILEIKCGKKSFEQALIGEIPKYYMAQMQHCMFVANVKRTHYFCYNESSMDKGILLEVKRDDNFIEDMIKKENEFYQMIINRTPPDGFEDIIYKTIDHGSELSCWLTEYAALQAQKKEIEQQEINVKEKIIELCGGKPHVGLGFKVKQNKRKGSIKYSSIPSLKDVDLEQYRGKETSYWTIKAMSE* 7 290 5.7694 33646.59 -2.5 0.68 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS096 0 IMGVR_UViG_3300030446_000094|3300030446|Ga0169454_10065948 APIS096.hmm IMGVR_UViG_3300030446_000094 ;;;;;; PF07728.19,PF08406.15,PF00004.34,PF13521.11 MQTISDELSRLQVIELDAGQIFSGNPSGTPIKGYATPSLYTPRLDPNYLFHESSRDIIVWLLSPEEPLYIFGPSGCGKTSCVKQLAARLNYPLFEVTGHGRLEFADLTGHLTVHEGSMSYEYGPLALAMRYGGICLVNELDMASPEVAAGLHGILDGSPLCIPENGGELIIPHPMFRFVATANTNGAGDDSGLYQGTQRQNLAFTDRFILCEMGYPDSSVEKKLLRDRYPTLPVSLCETMVDYANEVRRLFMGEVSGSSLSGSIEITFSTRSLLRWGALTLRFQPLAKQGIQPVTYALDRALAYRASRETRAMLHELAQRMFPHLTGNGSDLLMGEEALGFVKLHCQRSSAVPYPRIFLRKEYVLDGQTLTKDWVGDAGAEGLTITWGRTGQSGQQHFVPVDTCKDKSPILELTSRAAKKLREGYALIKTKSSI 5 434 6.4974 47810.64 0.0 0.7 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS097 0 IMGVR_UViG_3300032329_000792|3300032329|Ga0335329_10068837 APIS097.hmm IMGVR_UViG_3300032329_000792 ;;;;;; PF07275.16 MTTNTLKDTDTPSAWIGCLGCYNSGRLIGKWIPGIECNDLEAAGLTDSAGKCLRCNSDEFLAMDHEHFMGLLDGGEPNPQECYEAAEKLESVQEYERDILKAWLSNGMEFDLDEMRECYVGEFSTDEDMAQEYIDSTGLLSDVPDHLTRYFDLTSYARDMMFDMFEADGHYFLSR 7 175 3.8933 19873.02 -23.0 0.79 restriction-modification (RM) CLAN005 APIS003,ardu APIS098 0 IMGVR_UViG_3300033553_000842|3300033553|Ga0371479_1082023391 APIS098.hmm IMGVR_UViG_3300033553_000842 ;;;;;; PF07275.16 MTSPRIYIACLASYNNGVLHGKWIDATSDEDEMAEEVAGLLRRSPYPNVMVEDPETGEEVPSAEEFAIHDHEGLGNIGEYTPLSEIAALVEVLEENPDLPPHIVLAYHHEYNANPQDNYRGQYDSWADFAEQFEEETGGLEEIPDRFRYHIDWESVGREFETGGGLVEYSGHFFHTP 14 177 3.9625 20044.76 -25.5 0.83 restriction-modification (RM) CLAN005 APIS003,ardu phrog_14321 APIS099 0 IMGVR_UViG_3300050134_000048|3300050134|Ga0500026_0000319_27356_28222 APIS099.hmm IMGVR_UViG_3300050134_000048 ;;;;;; PF09588.15 MKYKKVNVEQGSKEWLNWRKSVITATDCPSILGTSKFQTAYKCWQRKCSLIGEQTCNAAMERGSMLEPQARDNFIKKYGINMTPTVVESKEYEFLGASLDGISDCGNYILEIKCGEKSYTMALNGEIPPYYVDQMQHQLLVTQAEKCFYYCYNGKEGKCLEVFKDPEFESKFLPIARKFWKGVACFEPPSLAASDYLDMNDNLSWLKYATMYQEVEASIKSLEERKDYIRKKLIELCGDNSCQGYGVKVISTLMKGRVAYDEIPEIKGIDLDKYRKGTTKTWKILIDK 47 288 7.8548 33110.18 3.5 0.78 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS100 0 IMGVR_UViG_2838756947_000002|2838756947|2838760980 APIS100.hmm IMGVR_UViG_2838756947_000002 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Xenorhabdus;s__Xenorhabdus innexi 626 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Xenorhabdus Xenorhabdus innexi PF03230.18 MSSEREPITKKEVSDAKRMQFLPHHFGRYFMNAETYLYNWMDKYATDYNGDYYSYYELSNGGILICPAKRYSLWSPNGESFDDLSSEQVGITVTLFLLNHFMFKTYEQEQRMAEDFSRKYEGLLDYGAGLGQEIWTPIRALID 3 143 4.6547 16857.90 -5.5 0.87 restriction-modification (RM) CLAN011 APIS007,ArdB APIS101 1 ugi DNA repair Cone et al., 1980 https://doi.org/10.1016/S0021-9258(19)70472-4 YP_009283008.1 https://www.ncbi.nlm.nih.gov/protein/YP_009283008.1 APIS101.hmm Bacillus phage AR9 GCF_001743835.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Firmicutes Bacilli Bacillales Bacillaceae Bacillus PMID: 6776115 https://pubmed.ncbi.nlm.nih.gov/6776115/ Uracil-DNA-glycosylase inhibitor. PF18880.5,PF18880.5 MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML 82847 83101 + 80397 86369 3 84 3.8702 9475.72 -11.5 0.79 DNA repair CLAN077 APIS101 phrog_26433,phrog_26433 APIS102 0 DQ003642_00031 APIS102.hmm DQ003642 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Listeriaceae;g__Listeria;s__unclassified Listeria species 1637 Bacteria Firmicutes Bacilli Bacillales Listeriaceae Listeria unclassified Listeria species PF06114.18,PF06114.18 MNKTSSELKQEFPELNFIIDNSLPTKLFGFIQNKVVHLHPSLTESELRCTIIEEAMHWKYTVGDITNFNNIDNIKQEKFARRKSHEYLVNLQTLALCYDLGYRTYYEAATFLNVTEKFLIEVVENYREKYGLMYNNGNYIIHFGPTIQVFQEDNSFYPYDYGC 24510 25001 - 22067 26851 14 163 5.2489 19313.85 -3.5 0.8 SOS response CLAN029 APIS132 APIS103 1 NTases CBASS,Pycsar,CRISPR–Cas (type III) Ho et al., 2023 https://doi.org/10.1016/j.celrep.2023.112305 YP_009031408.1 https://www.ncbi.nlm.nih.gov/protein/YP_009031408.1 APIS103.hmm Bacillus phage BCP1 GCF_000918315.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:36952342 https://pubmed.ncbi.nlm.nih.gov/36952342/ A family of bacteriophage nucleotidyltransferases (NTases) that synthesize competitor cyclic dinucleotide (CDN) ligands and inhibit TIR NADase effectors activated via a linked STING CDN sensor domain (TIR-STING).  PF10127.14,PF10127.14 MKTVDLSVTGMSWMEERTILLTPYGSRLYGTDTENSDWDFKGVCIPPKEYFLGLETFNEYNNTGGKTFKNTKDDVDINIIHVSKFVKDAMHGVPNNIEVLFAREQDYIILTELGQVLRDNRHLFLSKQIITKFGGYTRSLTNKLKNGAGRQELVEEFGYDTKNFMQGVRLQLSAIEILETGDYSTYRPERDFLLGCRNGEYTREQALALVESYDERLQVAHENSKLPEKPDYNKINGMLMAINEDALKFGIHS 93152 93913 + 88787 96486 73 253 5.0687 29034.82 -5.5 0.89 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS104 1 narp2_nampt NAD+ reconstitution pathway (NARP) Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 NP_899510.1 https://www.ncbi.nlm.nih.gov/protein/NP_899510.1 APIS104.hmm Vibrio phage KVP40 GCF_000843785.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 Nicotinamide phosphoribosyltransferase activity (Nampt), capable of producing NMN from PRPP and nicotinamide. PF04095.21,PF18127.6,PF04095.21,PF18127.6 MLNLNQNIAIATDSYKVSHWSQFPRGLEYSQYYVESRGGKFDKIMVDGMAYMCRILEKGVSMNDVKRAKRLFKKHFGSEVFNDKGWDIIVNELKGKLPIKIRAVKEGTVVPVKHPILTIENTDPRFGWLPGYLETFILRALWYPTTVATISFEVKKIIRQFMKKTVDDERIAEQEPFKLHDFGSRGVSSGESAAIGGSAHLKNFLGTDTVEALVAVEELYAEDVEDFIAGFSIPAREHSTTTIYKEAGEDQAFLNSIEQWGAALYACVMDSYDYEAAMNRVSTGRFKELIISKGGTFVARPDSGVPVDVVMKGLEILGKNVGYTINSKGYKVLHPSYRIIQGDGVNIEEIRRILSYMESKGWSAENIAFGMGGGLLQQLDRDTQRFAMKMSAAIINGEYVSVFKMPKTDPTKASKAGFLDLIAVDADNPNPAARGYVTFSSEDYDNRVHPKSVMQTIFEDGVTVADFSLEEARKLSDVQADFLNEGEWKIAKKIQTA 138214 139707 + 134159 142260 240 497 6.1441 55551.39 -2.0 0.92 NAD+ reconstitution pathway (NARP) CLAN043 APIS104,APIS197 APIS105 0 MN988543_00081 APIS105.hmm MN988543 d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Hyphomicrobiales;f__Rhizobiaceae;g__Rhizobium;s__unclassified Rhizobium species 379 Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Rhizobiaceae Rhizobium unclassified Rhizobium species PF07728.19,PF08406.15,PF07728.19,PF08406.15 MTVATESDDMITCKIDGGKTHSIRVYLRENYPDWTVDKYKATYPGEPVLSERGKRAALKAKEAAERKAAAQTFQPGQAFAFAERSLAEIFDLDASTALSSTGNPIMLRCLTEHHPEADAYVNPADPDYVFNIDLVKKVCLGFELGMNVYLWGFHGTGKTTVLEQCAARTGRPFLRVQHTGNTEEAHILGQYVVKTVMIDAQELGADGRIHSVQKPQTVTEFQYGPLAMAMKYGMVYCADEYDFAMPSVIALYQPVLEGKPLVIKDAPIDQRVIHPHPDFRFVATGNTNGVGDETGLYQGTMIQNAASYSRFHITEEVKYMDAKQESLVLRSKAGLGKNDADKFVKVANVIRESFSKGELSMTISPRELITAASLCIVFGNNPTLGFKLAFINRCSRVDQVTVEQVVQRHFA 65262 66497 + 59934 71493 37 411 6.5704 45524.92 0.5 0.79 restriction-modification (RM) CLAN004 APIS166 phrog_249,phrog_249 APIS106 0 OP480062_00009 APIS106.hmm OP480062 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Erwiniaceae;g__Erwinia;s__unclassified Erwinia species 551 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Erwiniaceae Erwinia unclassified Erwinia species MLATIKEIMAEAAGLPECDEIFSEKHKYVVFAHDSILDLKEWTAGDFFRVDDQVYVMGSKGQFRRANNHPANPWRVEMGMLYHENPGYHKKKIAKRNYGSWGKILEELEELNDAHEQGSKIMQLVELSDLYGAIEGYLEERFPGMKMHDLKKFSDITKRAFRSGERK 4 167 6.6110 19401.13 0.5 0.51 TIR-STING CLAN019 APIS150 phrog_6408 APIS107 1 dam restriction-modification (RM) Schlagman & Hattman, 1983 https://doi.org/10.1016/0378-1119(83)90098-7 NP_049647.1 https://www.ncbi.nlm.nih.gov/protein/NP_049647.1 APIS107.hmm Escherichia phage T4 GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:6307815 https://pubmed.ncbi.nlm.nih.gov/6307815/ DNA adenine methylases methylate adenine residues in specific sequences, the methylation site of the host Escherichia coli dam+ methylase. Methylation protects the site against cleavage by the MboI restriction nuclease. PF02086.20,PF02086.20 MLGAIAYTGNKQSLLPELKSHFPKYNRFVDLFCGGLSVSLNVNGPVLANDIQEPIIEMYKRLINVSWDDVLKVIKQYKLSKTSKEEFLKLREDYNKTRDPLLLYVLHFHGFSNMIRINDKGNFTTPFGKRTINKNSEKQYNHFKQNCDKIIFSSLHFKDVKILDGDFVYVDPPYLITVADYNKFWSEDEEKDLLNLLDSLNDRGIKFGQSNVLEHHGKENTLLKEWSKKYNVKHLNKKYVFNIYHSKEKNGTDEVYIFN 16846 17625 - 14685 20369 110 259 9.2073 30416.80 9.5 0.87 restriction-modification (RM) CLAN026 APIS107,APIS152 APIS108 1 ardc restriction-modification (RM) Belogurov et al., 2000 https://doi.org/10.1006/jmbi.1999.3493 WP_099065648.1 https://www.ncbi.nlm.nih.gov/protein/WP_099065648.1 APIS108.hmm Enterobacterales d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 91347 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales PMID:10686096 https://pubmed.ncbi.nlm.nih.gov/10686096/ This anti-restriction protein is able in vitro to protect the single-stranded DNA against the activity of type II restriction endonuclease HhaI. PF18818.6,PF08401.16 MTMNLHTQTSVPNPAPATSASPLEQSGSSKTKFSKGKAKPDVYQVVTDSIIEALETGVKPWVCPWKRNGAVSGIPSNFTTGTSYSGINIMMLWYSAAAQGFTDSRWLTYKQAQELGAQVRKGEKGTTAIFYKMLEKETEAGEVEKIPMLKSFTVFNAEQIEGLTLEEKTAPQPVAEFDPLPQVEALFQRTGAKITERGQQAFFRPSTDEIWMPERHLFTDAANFYATGLHELVHWSGAKNRLNREKGGKFGSAGYAFEELIAELGSAFLMADLSIYGEVQHENYIASWLEALKGDKRFIFKAASAASKAHRYLMDF 259 316 6.7658 35048.74 1.0 0.76 restriction-modification (RM) CLAN078 APIS108 phrog_7245 APIS109 0 IMGVR_UViG_3300033148_004419|3300033148|Ga0366832_10019799 APIS109.hmm IMGVR_UViG_3300033148_004419 ;;;;;; PF09588.15 VKTLDIEQRSDLWLTWRKEGIGASESSALLRMSKYDSPMDIWEQKTARAFPKIVNFDMQRGINLEEEARHKFQSLMGRMFFPICGERDDLPFVKASFDGIDCNHEYFVEIKCPRSDKLGDALDSGIIYRIKEEFPHYWCQVQHQYAVCDTAKVGYLAAYLGGEINYLHIPRDDTFINNTLIPEITRFWNDHVLADKEPELTDADYLYIDDAEAISIANEWKGINEELKALQEKEKGLRKRLIEHGDDGNVIIGNVVKLTRYATTRINFKMACIDNEIDMEKYKKTTIGCYRFTPIK 4 296 5.1623 34400.18 -6.5 0.78 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS110 0 IMGVR_UViG_3300007520_000028|3300007520|Ga0105054_100008822 APIS110.hmm IMGVR_UViG_3300007520_000028 ;;;;;; PF07728.19,PF00004.34 MAYGKIFKMAVAPDVRAAANQMEQFIDRRSAGRAGFSKAWDVFDQYIMDTFAAGLPYPVADAHADMSAWWNSASPSWAEEYGVYDISTSKLASYIGSPYIALVQIAAMGMLIDAREQGNKTLIDMSEFFSNSATMSLMCMDNALESTSGKRTYGLPDILPLNEGFTGYPFTHLVTGWLKNNFGVDLGAPPRGFKGILEESTKPVNTSGLGYSTSPMALGIISAPITKGTSMQDDVKNSASDNKAMIPTENTANEGLAEASRLKAQMETIAVSLKGLDPSLKSAIDGMLKAAGIGDVALLTEAVETASQSAGLIKLAEDKTSTVEAELISAKTRLAKLSSVASAPSGPVKVAGHGDIPDGDIVLKNAAVVFDIPKDAAKLFDFDIPCGEWDHPHPHVPAIDHDYVFDAEILVTMLIAIRKNQVPWLKGHTGTGKTTMIEQIYGRLNIPVFRVNLDSDISRGDLVGREVLTTDAAGKTITKFIDGVIPMAMQQPCCLLLDEIDASRPDLGFVLQRLTEGAGFMLLEDGGRTVEPHPYFRLAATANTNGRGDETGLYSGTRALGVALLNRFKPFIDVDYMTQSEEEALIADRVPGIPSDTAKRIASYASEHRKAFIASSVTLPNSPRDTIAMAWAFTDFHMFGPDRAMQMAFKYCVLAAADADDRQVLLGLAQRVFPDKVTKGLKA* 4 684 4.9403 73582.99 -13.5 0.55 restriction-modification (RM) CLAN004 APIS166 APIS111 1 mom restriction-modification (RM) Kahmann et al., 1985 https://doi.org/10.1016/0378-1119(85)90108-8 NP_050657.1 https://www.ncbi.nlm.nih.gov/protein/NP_050657.1 APIS111.hmm Escherichia phage Mu GCF_000837225.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:2934296 https://pubmed.ncbi.nlm.nih.gov/2934296/ Methylcarbamoylase for DNA modification. MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGRAGVVYQASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGVYLRANKERAVVHKFNQYRYIRFLNKRARKRLNTKLFKVQPYPK 35877 36602 + 32975 36602 155 241 10.4373 28277.51 22.0 0.8 restriction-modification (RM) CLAN079 APIS111 phrog_1384,phrog_1384 APIS112 0 IMGVR_UViG_3300028901_000217|3300028901|Ga0310362_10263355 APIS112.hmm IMGVR_UViG_3300028901_000217 ;;;;;; PF09588.15 MEQGSLEWLEWRRKGIGASDMAAILGVSPYSTPYQIWCEKTGRSTGFVGNFATQRGTELEAKARARYELISLEDMPPALAMHPKYEILRVSLDGRSADGHRILEIKCPGIQSHATAVAGQVPDHYIPQVQFQLAVTGADACDYFSFYNDNHALVEVRPDVEYQGMLVVKALDFWDLVKASTPPPLTDRDDKVIDTGEVFEICRELAEKKDVLKKTDLEKMKERVVLLGGHSRVRCGRVLVTRSRLASGKDSYRLTVSKDAEHA 4 263 6.6043 29411.55 0.5 0.82 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS113 1 antiDnd_p0020 Dnd Piel et al., 2022 https://doi.org/10.1038/s41564-022-01157-1 QZI91757.1 https://www.ncbi.nlm.nih.gov/protein/QZI91757.1 APIS113.hmm Vibrio phage 44E38.1 GCA_020477405.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:35760840 https://pubmed.ncbi.nlm.nih.gov/35760840/ Two genes (p0020 and p0021) provide resistance to the Dnd system (anti-Dnd), p0020 encodes a protein with a phosphoadenosine phosphosulphate reductase (PAPS) domain and a DNA N-6-adenine-methyltransferase (Dam) domain. MARARNIKPAFFDNDELADNDPLGRLLFIGLWTIADCNGNLEWRSKRVKKQLLAYDECCIDSLAINLDKSGFVRFYSDGDKIYLNVINFDKHQNPHKNEKAKGTEIPEYCEEYRQAIDLNKLAINRDLSGLKPNDSDSNPADSLNLIPDSLNLIPDTVTEQKPSPAKAGSDVLEIFNYWKEVMKKGGTTRLNKKREKLISDRLKEGYQVDEFKTAIFNCSMSPFHMGQNDNQTKYNDIELICRPDKFEQFRDNVGQQAQPRQMSKATERTFNNIIDVELD 26140 26982 - 20414 30247 19 280 5.2869 32180.26 -3.5 0.46 Dnd CLAN044 APIS113 phrog_21472,phrog_21472,phrog_12556,phrog_12556 APIS114 0 IMGVR_UViG_3300031577_000006|3300031577|Ga0316602_1000025150 APIS114.hmm IMGVR_UViG_3300031577_000006 ;;;;;; MYVGAANLARARNIKPGFFTNDELGECEPLARLLFAGLWTIADREGRLEYRPKKIKAEVLPYDDCNIIELLESLNEKGFITIYEVDKTKYVQVNNWTKHQNPHVKEGASNIPAPDQNSTSTVQEPEKNESSPADSLNLIPDSLNLIPDSLPTAAADEPKARTDYQGILDEYNATCTKMPKAEALTDNRRKTINARIKEHGRDAITEVFRYASQSPHHNGTNDLGWQASFDWLMGPKNFLKILEKARSGTPPGQKKLTGAAKFKKVLERGSFEQRGNYGDIDINCEHIPEDS 13 291 5.9906 32629.62 -2.0 0.44 Dnd CLAN044 APIS113 phrog_345,phrog_19658,phrog_12556,phrog_10469,phrog_16167,phrog_17247 APIS115 0 MN047793_00257 APIS115.hmm MN047793 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Lysobacterales;f__Lysobacteraceae;g__Xanthomonas;s__unclassified Xanthomonas species 338 Bacteria Proteobacteria Gammaproteobacteria Lysobacterales Lysobacteraceae Xanthomonas unclassified Xanthomonas species PF00293.33,PF01467.31,PF00293.33,PF01467.31 MSTNTTATQQYDLVTFIGRLQCPHKGHIETILSALEYGTKVLVVLGSHQSPRTFKNPWTTKERAQMVIDSLTVEQQARVLFVGAEDFLYSDADWFTNVSRLIRDVAQAEFGPDARTALIALNKDESTYYLNYFKNSMDMLTMREVKVGGDDAPSLSATKIRELYFEGYLDFISQVVTPGTLNFLKEFYKTEAYAELRTEYDDAVAYQKMFENVPYGNTNFLTVDSIVVQSGHVLLIQRKESPGRGLWALPGGHLNNNERFLDGAIRELREETGLKIPEKVLKGSIFHEQVFDHPDRSLRCRVKGKRGRTVTMAFGFKLDDAEKLPRVKGLDDAAAARWIPLEEVMDTMRDQLFEDHWDLIKRFSNLL 122606 123709 - 120313 126230 6 367 5.9068 41914.66 -3.0 0.88 NAD+ reconstitution pathway (NARP) CLAN020 APIS170 phrog_1185,phrog_1185 APIS116 0 IMGVR_UViG_2645727582_000005|2645727582|2646091902 APIS116.hmm IMGVR_UViG_2645727582_000005 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter baumannii 469 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter Acinetobacter baumannii VVKKLRLSIKDRLKLLRRAVIVAFMPNVRQVNVIWQEEVGDIKQHAFFVDGINPTDMTFLYFSYVAGNSDTFEDLTRRTKFLLNKIAVAYALYNKVSSKSIQYTPEPFRLVRKLNLHLLERNFDPDLYENIVLNHEEIVLTVYLHCLNGKLVGYQQYRPDCNDKRTNNPKLARYFTYKTPGNIAVWGLETLDYSRKRLYIVEGIFKASALHMLGHNAVALLTGSPSIDMLNWLRSLPFELYAIGDNDDTGKRLVRAVGNGTCFEKDVDEYSLEELESLLGSSQI 6 284 8.5611 32692.64 6.0 0.64 Retron CLAN025 APIS094 phrog_849,phrog_14043 APIS117 1 mga47 toxin-antitoxin (TA) LeRoux et al., 2022 https://doi.org/10.1038/s41564-022-01153-5 YP_010844222.1 https://www.ncbi.nlm.nih.gov/protein/YP_010844222.1 APIS117.hmm Escherichia phage SECphi18 GCF_002990915.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:35725776 https://pubmed.ncbi.nlm.nih.gov/35725776/ DNA polymerase PF00476.25,PF00476.25 MKDFERLFLDTETFSDVGLKKVGAYAYAEHPSTEIMICTYAIDEGRVQTWDATESPTMPRELRKALRRVSRKKAKIVMANGLLFDRLVIREKWGIDLPVSQIEDTMIMAFRHALPGSLDMQCQVLGVDAEHAKDKAGKALIKRFCKPTPKTYKIRRYTRETHPEEWAKFLRYAALDIVAMREVYWRIPDWGNSPKEDEILLIDQIINDRGFYVDVDLANAAIKAVRAHKEELKEEAWERFGGKLTGNDFLPILRDIAPAFTIHNAQKSTLNDLLEDPDFPDEGKALIEMRLGASSTASTKYNPLVNGLSADGRRRGCIQYGGAKRTLRWAGKGFQPQNLARGEYSDDHEGKIKRREGESDVSFWVRSHMLTNGINSLLRGTAHWAYDISKLTASTVRGCIIPAKGKKFVVADYSNVEGRGLAWIAGEKTALMVFKAGRDIYCETAGKMFGLDPDYIKANRKDLRQIGKACELGLGYGGGVAAFLQFAKNLGLDLYTMADVMKGTFPDHIWAAAKRGYEYARINEAKRPPKPGKKDERPTYILPKNVWLTCDAIKRMWREAHPKTVAFWAELEDAVLCAIRNPGKAYWAGANVRPDGRKALKIVRTKAKHDPTFDEERDDPNAAGWWLKIELPSGRIMSYPGIALSVTTEIDEDTGKKRTSTRIKYQGENQTTRQWGFQYTYGGKLTENIVQALCRDILAWSMPGVEAAGYEIVLSVHDELVTEVPDTDDYTTEELCALMCDLPIWAKGFPLAAEGDCMYRYRK 38357 40648 - 35507 43603 270 763 8.5680 86190.76 15.5 0.87 toxin-antitoxin (TA) CLAN080 APIS117 phrog_7766,phrog_7766,phrog_10285,phrog_10285 APIS118 0 IMGVR_UViG_3300046616_000314|3300046616|Ga0495668_0000005_189584_190411 APIS118.hmm IMGVR_UViG_3300046616_000314 ;;;;;; MEIDTSKLLNIDYLKETGAEPSWFGLGFIQLKLNSHQRVHFWSPQLEADVGDEEIHDHRYFFNSTILKGSLEQTLYHFEVDTHGMWEMRHVSCTPGETAPTHRIRGNPHEVSRQNMTAGCSYSIASEVFHTTHVEQDTVTFLQRKFGSHNVKEFARVIAIEGAPEVCPFSNPKPVAELWEWIAEIAKPNSVAKPGYHKAFIQKGTLGEASKIKEEIEEFFDALDQGVHIMALVELSDLIGSISAYLEKHHPQLTIKDLIAMSDVTKRAFLNGHRN 5 275 6.1492 31207.27 -3.5 0.68 TIR-STING CLAN019 APIS150 phrog_6408 APIS119 1 Ulx restriction-modification (RM) Piya et al., 2017 https://doi.org/10.1111/mmi.13705 YP_006478.1 https://www.ncbi.nlm.nih.gov/protein/YP_006478.1 APIS119.hmm Escherichia phage P1 GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:28509398 https://pubmed.ncbi.nlm.nih.gov/28509398 Ulx is a protein included in the P1 antirestriction system. It acts as a chaperone or packaging factor to increase the amount of DarB incorporated into the virion. MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLLKNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKFYRYDRPALTLIAATLYSHERFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT 9995 10435 - 1518 20332 51 146 5.0501 17080.31 -3.0 0.89 restriction-modification (RM) CLAN081 APIS119 phrog_32226,phrog_32226,phrog_1629,phrog_1629 APIS120 0 MGV-GENOME-0252771_51 APIS120.hmm MGV-GENOME-0252771 d__Bacteria;p__Firmicutes;c__Negativicutes;o__Selenomonadales;f__Selenomonadaceae;g__Mitsuokella; 52225 Bacteria Firmicutes Negativicutes Selenomonadales Selenomonadaceae Mitsuokella PF09588.15 MAKMIMTVVEMADRDAWLKMRAQGIGGSDAGTIVGLNPWKSKYELWLEKTGQVVPEDISDREPVYWGNRLEDIVAQEFTRQTGKKVRRHGMVQDEAYPFLFANVDRMVAGEKAGLECKTANGFKASLWEGDEVPASYYCQCQHYMLVTGLPIWYIACLVGGQHYVYKPIKRNEEDIQTLLEMEKAFWKCVVDRVPPEVDGSASCTEALAERFRGGVAEGIELPSWAVAEVEAIRQLEVQKKELDAKIAASKNRLKELMGDHETAVWGTDDEGGRITWKTQKGRTSIDSKRLKADHPDIFDAYSKTGKPIRVFRIA* 12 316 6.0760 35553.66 -1.5 0.81 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 phrog_434,phrog_8474,phrog_1956 APIS121 0 IMGVR_UViG_3300002898_000261|3300002898|draft_100066429 APIS121.hmm IMGVR_UViG_3300002898_000261 ;;;;;; PF10127.14 MNFKQLLHTKEYDFLRTNEHLGDNIILLGLGGSHAYGTNVEGSDIDIRGIALNSKSDLIGLSSFEQVVHEGTDTTIYSFNKIIKLLLNSNPNVIEILGLKPEHYLYVTDIGQELIDNRHMFLSQKCIKSFGGYANQQLHRLKNGEKLRKDEQRLSKHMMHLIRLYIMCVDILEKEEIITYRENERDLLMGIRNGVYLTNGVICGDFWDILRGYEWEFEYAKLNTNLPKKPNYKQVEEFVMNVNERVVKGSV* 70 252 6.5863 29086.37 0.5 0.9 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS122 0 IMGVR_UViG_3300036760_000193|3300036760|Ga0373620_0000070_103024_103785 APIS122.hmm IMGVR_UViG_3300036760_000193 ;;;;;; PF10127.14 MKEREMAERNLILKVMVGSHLYGTETENSDKDYVGVFIPDKEYVMGLKTCEQVEIRTNPSDSGHRNDKSDTDCTLYSLPKFIKLCAENNPNIVEILFADKKHIINCDPYGAELLMAAPLFISKKVKHRFLGYAHSQKQKLLFKQAEGGRVEYYEKFGYDVKFASHLIRLLTEGLELLVDGRLSFPITHNRLVRDIKIGKFKIEEVLSKADQFESLIEEAYVKSQLPYGPDLDKINDLQMQLLERYWDDQKVVS 7 253 6.3914 29230.62 -0.5 0.9 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS123 0 IMGVR_UViG_3300037330_000139|3300037330|Ga0400266_0002527_1772_2668 APIS123.hmm IMGVR_UViG_3300037330_000139 ;;;;;; PF09588.15 MTEPHEIQGSPEWLEWRKTKVTASELPIILGISKWATAYSLWQQKLGFTGGVKDNYAMKRGRDLEPMVRDLANENLSAKFVPAVLVSNDLAWAAASLDGIDREYEDGAIMEIKCPGLADHQLAEKEEVPPHYYPQIQWQLFVADLNTCYYVSYYDQSLAIFEVVRDDDYISNTLLPAGAEFYRCLVEMEEPAKEEDDFIQIVDPQFEENAREWKAAKELLTLYTEKEKYYKNKLISFTDDSNCKGSGITLQRIARDGSVDWKKRWADMKTQFPQAEEAYPESEYRKESIGYWKVSQDK 3 298 4.4297 34480.78 -16.5 0.74 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS124 0 IMGVR_UViG_3300037502_004185|3300037502|Ga0310151_0000105_71832_72464 APIS124.hmm IMGVR_UViG_3300037502_004185 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Vibrionaceae Vibrio PF18788.6 LKLRNNSKFALDQSHNYVVVDFDNVTERGLKSLITALKQAGATVTDVEASNKKTRRDGETVKRAKLFFDNGQSMTLFVGDEGDIYQMTLNNTKQPIPSVKNERELAREMVRLMERNQSKFDKQVARKAAQAVKDTSDIQPATRSIAKRLEEAKAAEQVAQSNYTNALAARDSVRTKLEEDQRRVADLEAALEQEKQETKELEAQLEAAKA 6 210 9.2927 23615.53 2.5 0.56 restriction-modification (RM) CLAN030 APIS143 phrog_1620 APIS125 1 U56 toxin-antitoxin (TA) Arturo et al., 2024 https://doi.org/10.1016/j.molcel.2024.05.001 YP_009986712.1 https://www.ncbi.nlm.nih.gov/protein/YP_009986712.1 APIS125.hmm Escherichia phage Ukendt GCF_010121085.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:38788717 https://pubmed.ncbi.nlm.nih.gov/38788717/ 8BAU Retron-Eco1 inhibitor PF08719.16,PF08719.16 MRITDEYVFFFSHKDVFSNWYIAPFTETEPGYNETFCCVEQYMMWRKALLFKDHAIAAAILDHSTIRDNERKQAYYKRMGRAVSGFNNDMWEENRERIVMRGLCLKYVQNPDLYADLHLYQYKTFVEASPYDKVYGIGMGMYEPGVLNPATWKGQNLLGKYHNKLIEILFQDKIPQRRYL 26712 27254 + 23418 30149 81 180 7.7955 21504.66 3.5 0.88 toxin-antitoxin (TA) CLAN045 APIS125 phrog_1017,phrog_1017 APIS126 0 IMGVR_UViG_GVMAG-S-3300006056-154_000001|3300006056|Ga0075163_1001601612 APIS126.hmm IMGVR_UViG_GVMAG-S-3300006056-154_000001 ;;;;;; MIDYSLIQHSIFYYRSLGFTQIEAPWLVEERISNITKPANKKNYLLDNLALVGSAEQSFLQLLKDNVLKNDVCQKYIATTPCFRDDTIDNLHQRYFIKSEVIIIGLSTESLIPELEKLKLQALNFFKSFIPVEIADVDKLNNSFDIVANVKSTNAKNNTDNTSDKIEKVELGSYGIREVHFSNNNTTNYKTNNDNVKKVYYIYGTACAEPRLSTVLNAAKIHGYHNDFIPKGIVGSFAKIIEEFEETKDAYIQDNPIMELVELSDLVGAIELYLQKYNITMEQLLTMSHTTKRAFINGRR* 5 301 5.9760 34318.11 -2.0 0.85 TIR-STING CLAN019 APIS150 phrog_6408 APIS127 0 IMGVR_UViG_3300006925_000049|3300006925|Ga0098050_100007221 APIS127.hmm IMGVR_UViG_3300006925_000049 ;;;;;; PF07728.19,PF07726.16,PF00004.34,PF08406.15,PF13173.11,PF13401.11 MDTESRLKSTLDLSLELLIQLYDSLRSRHESKKYHMGEDKDEVEKLYKQYLDSEQETDYVLFALKEVHHADTRWNSLKAILASRESAGNAPSYEQIQPHDHEISEQLEHMCDLMRLPRLLSMENDEVETATPNDDNSLHVEAKTLDVVNTLMSSMGESLTLGDILDAANRGVINTINHKADMEEKDKTIQDLQKKVSTPSFPAGVVANSSPSANKNSTPITEADAEVVMQNAMDIFVNNQGKKVKALDYEVPTFKWKKPNPDVPELDPHYVFRPELVSDVLYCLLHNQKGYLSGHTGTGKTTLLEQICAKLGYPFKRINFDSEITRQDLVGREVLMSDSGSTKSKFIDGIIPQAVRTPTVLCLDEIDFVRPDVAYVLQRALENKGFTILEDGDRFIEPHPLFRIFATANTKGQGDETGSYQGARHQSLAFLDRFNVFTSVPYLRPIDERDLLVSSATGLDVKLAEQMVKFANEIRDAFKNGTIYMTTSIRGLMTCAKMYTFFLPMMSGNHNAALTFAITKSILNRCGHQDFANISEIAQRVFDTGSGTPLNFKYED* 7 557 5.0667 62589.76 -14.0 0.61 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS128 0 MT375530_00042 APIS128.hmm MT375530 d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Candidatus Pelagibacterales;f__Candidatus Pelagibacteraceae;g__Candidatus Pelagibacter;s__unclassified Candidatus Pelagibacter species 198251 Bacteria Proteobacteria Alphaproteobacteria Candidatus Pelagibacterales Candidatus Pelagibacteraceae Candidatus Pelagibacter unclassified Candidatus Pelagibacter species PF07275.16,PF07275.16 METIQKTQDNAPQIYVACLSSYNAGKLHGFWIEPATDKAELLGQIDKVLKTSPMPNAEEWAVHDYNDFPNLGEYPDLDKIIEVQEAITEHGADIVHAFLENWSVEDLDHIGDAYYGKYDNFTEFAEQYAHDTIEGLNDSSELSRYFDYQAYENDLQHDYYDGKAGDGSSLIFSCNW 34672 35202 - 33494 38598 30 176 3.9308 20108.83 -22.5 0.84 restriction-modification (RM) CLAN005 APIS003,ardu APIS129 1 Lidtsur-6 AVAST (Antiviral STAND) Gao et al., 2022 https://doi.org/10.1126/science.abm4096 YP_009821604.1 https://www.ncbi.nlm.nih.gov/protein/YP_009821604.1 APIS129.hmm Escherichia phage Lidtsur GCF_004800205.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:35951700 https://pubmed.ncbi.nlm.nih.gov/35951700/ Lidtsur-6, Lidtsur-17 and Forsur-7 were active in phage plaque assays and restored phage propagation on Avs-containing E. coli. MTKVTVLLTSDKVTLIALLGDAVEDTPSLALDIKPGSDLSAAIAELETQLKKPTAPIMFIVNGGGYSPEAAAQYSPEKVAEFTVDNPALDEFVAAVA 2223 2516 + 1 3663 4 97 3.9150 10076.54 -8.0 0.35 AVAST (Antiviral STAND) CLAN031 APIS129,APIS193 APIS130 1 RacC Retron Bobonis et al., 2022 https://doi.org/10.1038/s41586-022-05091-4 NP_415867.1 https://www.ncbi.nlm.nih.gov/protein/NP_415867.1 APIS130.hmm prophage of Escherichia coli str. K-12 substr. MG1655 GCF_000005845.2 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:35850148 https://pubmed.ncbi.nlm.nih.gov/35850148 RacC is a Rac prophage gene, function as retron TA blocker. It directly blocks RcaT toxicity, inhibit the toxin of abortive infection systems. MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDIDGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI 1417488 1417763 - 1413237 1419456 4 91 5.7121 10015.67 -1.0 0.74 Retron CLAN082 APIS130 phrog_4778,phrog_4778 APIS131 1 vcrx091 CRISPR-Cas evasion by DNA repair Roy et al., 2022 https://doi.org/10.1093/nar/gkaa518 YP_008997727.1 https://www.ncbi.nlm.nih.gov/protein/YP_008997727.1 APIS131.hmm Vibrio cholerae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:32556263 https://pubmed.ncbi.nlm.nih.gov/32556263/ CRISPR–Cas evasion by repairing double-strand DNA breaks via recombination between short sequence repeats PF00436.30 MSKGVNKVILVGNLGSDPEIRYMPSGTAVANFNVATTDTWRDKQSGEQREHTEWHRVVLKGRLAEVAGEYLKKGSQVYLEGSNRTRKWTDNQQIERYTTEVHCFEMQMLGGRGNAPQDNSQRAAPQQGQRTGAGTQSAPVQQSAPQGGMGGGYGPAPDGWDDDIPFMRLHHLAGG 313 175 7.4200 19154.20 2.5 0.6 CRISPR-Cas evasion by DNA repair CLAN083 APIS131 phrog_25370,phrog_44,phrog_12631 APIS132 1 MpaR SOS response Argov et al., 2019 https://doi.org/10.1038/s41467-019-13296-x WP_003721739.1 https://www.ncbi.nlm.nih.gov/protein/WP_003721739.1 APIS132.hmm prophage of Listeria monocytogenes 10403S GCF_000168695.2 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Listeriaceae;g__Listeria;s__Listeria monocytogenes 1639 Bacteria Firmicutes Bacilli Bacillales Listeriaceae Listeria Listeria monocytogenes PMID:31754112 https://pubmed.ncbi.nlm.nih.gov/31754112/ MpaR plays a critical role in the de-repression (induction) of the two phage elements and is sufficient for ϕ10403S induction. PF06114.18 MYEKLVNKYQDEVTIREEKMPYKLPGLYLNGMIFISKDQSSIEKGCVLVEELMHYKYTVGNITKQETIMDKKQEIFARRKGYEELIPLDDIIACFYLGLREYFEVAEFLEVTEEFLRHTVSHYAEKYGPMYDYGGYFINFGNSIDVYKKF 107 150 4.9109 17876.56 -4.5 0.85 SOS response CLAN029 APIS132 phrog_87 APIS133 1 vcrx092 CRISPR-Cas evasion by DNA repair Roy et al., 2023 https://doi.org/10.1093/nar/gkaa518 YP_008997728.1 https://www.ncbi.nlm.nih.gov/protein/YP_008997728.1 APIS133.hmm Vibrio cholerae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:32556263 https://pubmed.ncbi.nlm.nih.gov/32556263/ CRISPR–Cas evasion by repairing double-strand DNA breaks via recombination between short sequence repeats PF03837.19 MVSPNRSFVNWRNTMSDNKSLVTRIASRFGVDTRKFYETLKATAFKQRDGSAPTDEQMMTLLIVAEQYGLNPFTREIYAFPDKQNGIIPVVGVDGWSRIINEHPQYDGVEFVYSDKMVRMQGAKVECPEWIECVIYRKDRSRPIRIKEFIDEVYREPFQGQGRNGAYTVDGPWQTHTKRQLRHKSLIQCSRVAFGFSGIYDQDEAERIREMEQASAINPAIANLPSPSQVHSQEPLAIEHKELDPILTKLANRAIAEKAWSAAHEYVKGRYEGSELQYATQFLREKEMDQMEPPKPDYQESHEQESAAGGSANAELGAEEMPPLSDEDMIPVMEEEGAEGSYY 6 343 4.8053 39173.88 -11.5 0.57 CRISPR-Cas evasion by DNA repair CLAN046 APIS133 phrog_12233,phrog_252,phrog_8375,phrog_10056 APIS134 0 IMGVR_UViG_3300005662_003648|3300005662|Ga0078894_100068595 APIS134.hmm IMGVR_UViG_3300005662_003648 ;;;;;; PF07728.19,PF08406.15,PF00004.34,PF13555.11 MTAVNDRVAKAIAEHLGKTPTKPAVATVEMEKKPVVVAEDQKLFSEVFGYKPATGDFAVTILPDNSNPDIARLVPNIDTEYVVQQEQAALLVAGIEDGDKTLLTGPTGSGKSSLIKYVCAMLNRPFIRINMSGDVESASLFGTLVVRGGATVWEDGAVTEAAKHGAVCLVDEWELMPAEIAMGMQNLLEDGGYLYLKEKPGTSDDRTVNPHKDFRLVFAGNTTGQGDMTGAFTGVGIQNTATIDRFTNTIRLDYLSQAHEVKIITSKTNVADNVAKNMVRFAGLVRNAYDSGKLGLTVSPRTLINWAKKCQRYELSYALNVCYLCKLSADDSKSVLEMYNKVFGA* 3 346 5.4730 37387.71 -3.0 0.81 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS135 0 IMGVR_UViG_3300026487_000009|3300026487|Ga0256408_10002085 APIS135.hmm IMGVR_UViG_3300026487_000009 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Leclercia; 83654 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Leclercia PF18788.6,PF05911.16 MSLVSRNGKSYASLDFSQITEKGLKPLIDALNRNNVTVIQTSASNKATRKDSIQVKQATITLQDGQELLFQINDTGDISMVKLNGKVIPVHIGESIADVARSAGTAAARNSKKFTDALAAKAKRAISADLNKKASVKSTAQQIQEVKGQLADKKTSNIALEEQIKGVSSQKDKLSARIEQAKTELAQEKSTGTQLEMQLKELEKTLNV 6 208 10.4129 22465.62 9.5 0.57 restriction-modification (RM) CLAN030 APIS143 phrog_1620 APIS136 1 hia5 restriction-modification (RM) Drozdz et al., 2012 https://doi.org/10.1093/nar/gkr1039 AEV40923.1 https://www.ncbi.nlm.nih.gov/protein/AEV40923.1 APIS136.hmm Haemophilus influenzae JF268249.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pasteurellales;f__Pasteurellaceae;g__Haemophilus;s__Haemophilus influenzae 727 Bacteria Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus Haemophilus influenzae PMID:22102579 https://pubmed.ncbi.nlm.nih.gov/22102579/ hin1523, nma1821 and hia5 are all DNA adenine N6-methyltransferases. Plasmid DNA overexpressing these novel DNA methyltransferases was resistant to cleavage by many restriction enzymes sensitive to adenine methylation. MANQNTFKQAPLPFIGQKRMFLKQFEQILNENISDNGEGWTILDTFGGSGLLSHTAKRLKPKARVIYNDFDGYAERLAHIDDINQLRAELYSVVGNATSKNKRMTKDCKAECIRIIQNFKGYKDLNCLASWLLFSGQQVATLDDLFQHNFWHCIRQSDYPKADGYLDGVEIVKESFHTLLPKFSNDPKALFVLDPPYLCTKQESYKQATYFDLIDFLRLVNITRPPYVFFSSTKSEFIRFVNYMLEDKVDNWQAFENAKRITVNAKLNYQVAYEDNLVYKF 386 281 8.0150 32706.31 4.5 0.86 restriction-modification (RM) CLAN084 APIS136 phrog_23568,phrog_28718,phrog_8559 APIS136 1 hin1523 restriction-modification (RM) Drozdz et al., 2012 https://doi.org/10.1093/nar/gkr1039 NP_439673.1 https://www.ncbi.nlm.nih.gov/protein/NP_439673.1 APIS136.hmm Haemophilus influenzae Rd KW20 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pasteurellales;f__Pasteurellaceae;g__Haemophilus;s__Haemophilus influenzae 727 Bacteria Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus Haemophilus influenzae PMID:22102579 https://pubmed.ncbi.nlm.nih.gov/22102579/ hin1523, nma1821 and hia5 are all DNA adenine N6-methyltransferases. Plasmid DNA overexpressing these novel DNA methyltransferases was resistant to cleavage by many restriction enzymes sensitive to adenine methylation. PF02086.20 MSEYLEYQNAIEGKTMANKKTFKQAPLPFIGQKRMFLKHVEIVLNKHIDGEGEGWTIVDVFGGSGLLSHTAKQLKPKATVIYNDFDGYAERLNHIDDINRLRQIIFNCLHGIIPKNGRLSKEIKEEIINKINDFKGYKDLNCLASWLLFSGQQVGSVEALFAKDFWNCVRQSDYPTAEGYLDGIEVISESFHKLIPRYQNQDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRLINLTKPPYIFFSSTKSEFIRYLNYMQESKTDNWRAFENYKRIVVKASASKDGIYEDNMIYKF 386 296 8.0965 34485.55 5.0 0.84 restriction-modification (RM) CLAN084 APIS136 phrog_23568,phrog_28718 APIS136 1 nma1821 restriction-modification (RM) Drozdz et al., 2012 https://doi.org/10.1093/nar/gkr1039 WP_002212760.1 https://www.ncbi.nlm.nih.gov/protein/WP_002212760.1 APIS136.hmm Neisseria meningitidis d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Neisseriales;f__Neisseriaceae;g__Neisseria;s__Neisseria meningitidis 487 Bacteria Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Neisseria Neisseria meningitidis PMID:22102579 https://pubmed.ncbi.nlm.nih.gov/22102579/ hin1523, nma1821 and hia5 are all DNA adenine N6-methyltransferases. Plasmid DNA overexpressing these novel DNA methyltransferases was resistant to cleavage by many restriction enzymes sensitive to adenine methylation. PF02086.20 MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVFGGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLDPERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAADYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQYMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIYKID 386 279 9.1441 32375.94 8.0 0.88 restriction-modification (RM) CLAN084 APIS136 phrog_8559 APIS137 0 IMGVR_UViG_3300027863_000098|3300027863|Ga0207433_1000259650 APIS137.hmm IMGVR_UViG_3300027863_000098 ;;;;;; PF10127.14 MNVESWIEQRTILKVYRGSYAYGMSHTDSDVDLGAVCIPPRDYIIGYYNFEQYESKNYTNYPQYEEIRKPAEITNYGLHKFVKLATNCNPNIIEYLFVDPSDIIYCDDLGADLIQNRHLFLSTKARSTFGGYALSQLNKLISKDKSNHNSHGSHKDLIERYGYDTKHAQHLIRLLHMGIEVLTEGNVYVKRPDRAHLLSIRYGGYTLDDIKKEADELFKKLEDAYKNSRLPESPNVEKINKLLIDMTLRELEKWK 9 255 7.0179 29724.70 3.0 0.9 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS138 0 JN882284_00173 APIS138.hmm JN882284 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Cronobacter;s__unclassified Cronobacter species 413496 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Cronobacter unclassified Cronobacter species PF00156.32,PF14572.11,PF13793.11,PF00156.32,PF14572.11,PF13793.11 MSIELRLNNLVVPVDHFTFKGGEEQVKIDLKHAPKGGIGFVDITAKIKNSTDVMALAMLVDACSRLEGLHNHAEFTLHLPYIPYARQDRVMNPGEALSIKVFANIVNSLGFDTVIVDDPHSDVSAALLNNVRIRGQDVLISEMKDLGNSTLHSNSLVVVAPDAGARKKAHKVADRFSLPLVEAGKVRDLQTNEITGTAVFGDVEGKVCMIVDDICDGGRTFIALAQALKENGAKRVILYVTHGIFSFGKQVILDGGVDEIYAYHDWTENF 79935 80747 + 78089 83924 21 270 5.9792 29465.77 -3.0 0.88 NAD+ reconstitution pathway (NARP) CLAN027 APIS160 phrog_619,phrog_619 APIS139 0 IMGVR_UViG_3300031785_000173|3300031785|Ga0310343_1000130817 APIS139.hmm IMGVR_UViG_3300031785_000173 ;;;;;; PF07728.19,PF00004.34 MPTDNDTIISTLFPSATDNTNIPCKVFIDDLIVKYKDDRVAVKTLTRVFFNQCVETFGIPPSVLRLTPKGASESISLSKAIDKMNKGLLCSLILQASNHLAKSDPTQALQQVILELQDNIDDCGSNEEDEKGKKLFAYGVPFNTKPLDVRNNTHDTSQLGIGSKSFPAVALAKKLEVFFNEYASTSKTTTTTKKEKTMTFDVNKANDKVVAMAMAMAKHDIEDGLDTIENCVSKRLDKALQEYGNKHTLTSEERVSLKASVDLTMPTYTEEDIDRIFAENKDDAREYLPSDVEPNNEHPLGINDKEKVVPIKPVKIDKPELKPAVESMLSQMTSGVVTDIEELLQTKSQLAELQSSSENLKKELSTLRSRSFAAQAASTGEAKVDGKKLKYEVVMRKASDIFRNPRSGRKITQLDFEIPTLIWKDDEGKVVRHPMCPDTNVNYQFRASHLIKFLTAYLLGKNVWLHGHTGTGKTTLPEQVASLIGLPLFPLNLDSQLERADLTGQTNLIEQNGTTITKFEEGILPRAMVLPCFLVLDEIDAGKPDILFAIQRATEGKGLLLTEDGGRLVKPHPLFRFVATANSRGQGDEHGVYAGVRPMNGALLNRFGTFIEIDYMTPEEESALLEREYKLPKDITKNLVEFAKLCRKAFKSGETSVPVSPRDTTAVAEFYTHYSAVLTTKTQAIEYAIDNAILSRCPIDNRQRVVELASRCFADCKFTN 6 720 6.0114 80060.38 -4.0 0.55 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS140 0 MW250275_00006 APIS140.hmm MW250275 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Kosakonia;s__unclassified Kosakonia species 1330547 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Kosakonia unclassified Kosakonia species MATINVYSLSNGGLAFIAGDKVDEAPSLGVTPKPEASRDDVIAEVKGALEDIGGLLFILNGGKLSVEKLDALEPVFVESFEADNVDLDAFADA 2223 2504 + 1 4044 5 93 3.7299 9667.85 -12.0 0.59 AVAST (Antiviral STAND) CLAN031 APIS129,APIS193 APIS141 0 MT560058_00009 APIS141.hmm MT560058 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Pectobacteriaceae;g__Pectobacterium;s__unclassified Pectobacterium species 122277 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Pectobacteriaceae Pectobacterium unclassified Pectobacterium species PF00293.33,PF01467.31 MSLAYLRRIAEGDLLKRGARYMKYDKAVVIGRFQPFHNGHVHMVKEALAVADTVYIVIGSANLYPDVRNPFTSDERKHMIQEWINISGLSRKDRKRVVLTSVNDYLYNEQKWKMEVRKAIQQEDSEQIVIVGAEKGKDSYWLTEFGWKTHYITLLKSDGNVISASDFRNQYLRSDFVNGLYRKSDNIVFPISTIEFMEEFRDSPTYERLREEQLKYDKELEKFKDYPYPEALNCCTADSVVICNNHLLVIKRKFSPGKYAYALPGGHKNADETFLQCAIRELTEEVKMKVPTKVIAGSIRNSFMFDHPHRSAYFSKPTVAQYIVIEPNQDGSLPKVRGADDALDARWMPLHKVQENQAQFFDDHYQIIVAFIGI 71 374 8.1227 43390.55 7.5 0.86 NAD+ reconstitution pathway (NARP) CLAN020 APIS170 phrog_395 APIS142 0 MT740244_00062 APIS142.hmm MT740244 d__Eukaryota;p__Chordata;c__Amphibia;o__Caudata;f__Proteidae;g__Proteus;s__unclassified Proteus species 210425 Eukaryota Chordata Amphibia Caudata Proteidae Proteus unclassified Proteus species MFKDHLISRGFNPDEYHCWMSDDTLTVPLYCPNTGRFLGEQSYKPTSKRKYITHSTSYAFWGWETVPVGYNGICFIAESVFKAVALHNIGFVAIAALGVNLPKHVKDLLPNKDNVKYFLIGDNDVQGKKESKKFNGFELVSPVDLDEMSRKDLLEFIRGNSGYFK 44744 45241 - 41767 47158 3 165 7.8100 18797.48 3.5 0.9 Retron CLAN025 APIS094 phrog_14043,phrog_14043 APIS143 1 Hdf restriction-modification (RM) Piya et al., 2017 https://doi.org/10.1111/mmi.13705 YP_006495.1 https://www.ncbi.nlm.nih.gov/protein/YP_006495.1 APIS143.hmm Escherichia phage P1 GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:28509398 https://pubmed.ncbi.nlm.nih.gov/28509398 A key member in the P1 Dar antirestriction system. The absence of either Hdf or DarA results in failure to incorporate any of the other antirestriction proteins. PF18788.6,PF18788.6 MCQMTKNKYATVDFDQVNEKGLKSLITAINKTGVTVIEVDSSNRATTKDGVKVKTAKLVLSDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAARKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSFNQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN 29825 30436 - 24244 34318 82 203 10.9850 22151.46 21.0 0.59 restriction-modification (RM) CLAN030 APIS143 phrog_1620,phrog_1620 APIS144 0 IMGVR_UViG_3300050015_000276|3300050015|Ga0494705_0000020_135416_136201 APIS144.hmm IMGVR_UViG_3300050015_000276 ;;;;;; PF10127.14 MFNIKDFHLGDRVGNYILVGQLGSKAYGTNTPESDDDFTGVVVAPLSHYIGLKKWENDGTLKIDLKESHNVEMTAFDVRKFIRLALAFNPNIIPLLYLRQQDYEIITDGGQKLISVRDAFTSKRAYATMIGYAYSQRHAVVNCNTGKLGKKRKDLVAKFGYDTKYASHTIRILNMAIEFFRDGKLNVYRAFDRDELLDIRQGRWTLGQWISEVDNLLTKAKEAEKESNLPETPDFERVNDLCMELIETYAKDDWNYVYDGT 8 261 6.3533 30006.06 -0.5 0.89 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS145 0 MW749010_00040 APIS145.hmm MW749010 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Hafniaceae;g__Hafnia;s__unclassified Hafnia species 568 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Hafniaceae Hafnia unclassified Hafnia species PF00156.32,PF14572.11,PF00156.32,PF14572.11 MKATYKVFIQNCEGETFEINVEEVTFPSGEVSIHLEEGSLSACGSITRFLIVTRGYEPDQLFKVALIKDALRGMILIDQPLIIQNHWTLVLPYLPNARYDRRMVDNDSLALKVYSEMLNSLGFDEVISYDVHSDVAHSCINNFNHVPVWEFIKGLDTLHNVSGEYTALVAPDNGSTKKVEKVADVLGLPIVQMTKRRDPATGKLSAPFVLHGLEHLKGGKLLIVDDICDGGYTFIQATQLLKVFDVEKVGLFVTHGIFSKGVDALFNAGIDEIYTTDSLRMPEEFDSRIKGMELIKV 28306 29199 + 26275 34730 6 297 4.9484 33140.13 -8.5 0.87 NAD+ reconstitution pathway (NARP) CLAN027 APIS160 phrog_619,phrog_619 APIS146 0 IMGVR_UViG_3300025528_000007|3300025528|Ga0207867_10002597 APIS146.hmm IMGVR_UViG_3300025528_000007 ;;;;;; PF07275.16 MRVYIACLASYNAGILHGEWIDLDGLDADDLREKIADMLRRSPCPNVEVDCPECDGTGNANRDPHKADGCLRCGSTGKVPSAEEWAVHDYDDFPNLGEYPSIERLIEVAEAISKHGEAMRVWLDSTGSVEGFEDAYYGTAESWEDFAARLLEETGALAEVPEHLQYYFDYAAYARDLKTDGWWAEYGADGQMHFFSA 24 197 4.0545 22005.17 -22.0 0.83 restriction-modification (RM) CLAN005 APIS003,ardu APIS147 0 IMGVR_UViG_3300027857_000008|3300027857|Ga0209166_1000054018 APIS147.hmm IMGVR_UViG_3300027857_000008 ;;;;;; PF07275.16 MRTGPHIYVACLEAYNNGMLHGEWIDAAQDVGGIRADVQRMLADSPISNELHTCEEWAIHDYENFEGLRVDEWSPLEYISEVALAMNEMPEPGLLCGILDHLGSGTSVQDAKDYIEDNYQGTWKTLEEYAENYFNDTHGKIDDWLYNYIDFERMGRDFEYGGDIFTISVDAGLAVFSNT 11 179 3.8927 20365.40 -23.0 0.85 restriction-modification (RM) CLAN005 APIS003,ardu APIS148 1 OrbA bacteriophage exclusion (BREX) LeGault et al., 2021 https://doi.org/10.1126/science.abg2166 YP_004250966.1 https://www.ncbi.nlm.nih.gov/protein/YP_004250966.1 APIS148.hmm Vibrio phage ICP1 GCF_000893175.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:34326207 https://pubmed.ncbi.nlm.nih.gov/34326207/ Clinical phage ICP1 overcomes cocirculating SXT ICEs through acquisition of epigenetic RM as well as an anti-BREX protein, OrbA. MKIANNYTFKQNKDGSVTVYNHGMFCAIIDEGMSRAKRIFLETV 10172 10306 + 7839 13138 7 44 9.0663 5042.84 2.5 0.31 bacteriophage exclusion (BREX) CLAN085 APIS148 phrog_11733,phrog_11733 APIS149 0 IMGVR_UViG_3300025415_000127|3300025415|Ga0208868_100032919 APIS149.hmm IMGVR_UViG_3300025415_000127 ;;;;;; PF03837.19 MSALVTLSNQLAEKFGMGNDSKVLETLKQTVFKSSASDAQMIALLVVANQYSLNPWTKEIYAFPDSKNGGVIPVVGVDGWSRIINDHQQLDGISFNESPTSIELNGSRPCPEWMECVIRRKDRSHPVIVREYLDEVYRPAYVDEKGRSRPGPWQSHTKRFLRHKTLIQCARLAFGYTGIYDEDEAHRIKDMGTIEIVALDTQEWIDKAGKAESLAELENVWKTGNQKFLDAKVDNKQFKYACNFRKKELIDRDNVEIIETKENNANNQPT 4 270 6.3540 30783.80 -0.5 0.66 CRISPR-Cas evasion by DNA repair CLAN046 APIS133 phrog_12233,phrog_252,phrog_8375,phrog_10056 APIS150 1 Atd1 TIR-STING Ho et al., 2023 https://doi.org/10.1016/j.celrep.2023.112305 APC44382.1 https://www.ncbi.nlm.nih.gov/protein/APC44382.1 APIS150.hmm Pseudoalteromonas phage PH357 GCA_002614885.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Alteromonadales;f__Pseudoalteromonadaceae;g__Pseudoalteromonas; 53246 Bacteria Proteobacteria Gammaproteobacteria Alteromonadales Pseudoalteromonadaceae Pseudoalteromonas PMID:36952342 https://pubmed.ncbi.nlm.nih.gov/36952342/ Functional screen identifies Atd1, a phage MazG-like suppressor of TIR-STING cell suicide MSGEGYHLKQIPKGVLGERSKIVEEFEEWRDACEQGCAVMELVELSDMLGAIDHYIKDNYNLYLGDLIKMKDITERAFENGRR 60473 60724 + 57983 63761 192 83 4.5577 9596.92 -5.0 0.79 TIR-STING CLAN019 APIS150 phrog_6408,phrog_6408 APIS151 0 KY979132_00178 APIS151.hmm KY979132 d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Comamonadaceae;g__Acidovorax;s__unclassified Acidovorax species 12916 Bacteria Proteobacteria Betaproteobacteria Burkholderiales Comamonadaceae Acidovorax unclassified Acidovorax species PF00293.33,PF01467.31,PF00293.33,PF01467.31 MSQKVGFVIMRAQPFHAGHAHLVKTARSKCDRLVIILGSANRPQSARNPWTYEERRKMIHLWVDAQGLSNIEFAPLNDHRYSDVTWRQEVQTVFDELSFPSDILILFGHSKEGNDYLEWFPEVNYVEVAAEPGMETLCATDLRRQMWDQGCLPSEAAADMQYFEDERARFSDYPYPETLNFNCADAIVECDGHVALIHRGGSPGKGNWALPGGFKNRNETFLDCAIRELREEVNIRVPEKVLRGSIVSTKLFDSPDRGEGIPRNTLAVHIRITRDANGDLPRISPGDDAMKATWVSIYSAMNELAMHDDHQDILSVMLGTSPVPAIFNPRFQPKR 112251 113258 + 110955 117722 56 335 5.7797 37984.94 -4.5 0.9 NAD+ reconstitution pathway (NARP) CLAN020 APIS170 phrog_3041,phrog_3041,phrog_19657,phrog_19657,phrog_1198,phrog_1198 APIS152 1 dmt restriction-modification (RM) Sistla et al., 2004 https://doi.org/10.1016/j.bbrc.2003.12.070 YP_006537.1 https://www.ncbi.nlm.nih.gov/protein/YP_006537.1 APIS152.hmm Escherichia phage P1 GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:14715260 https://pubmed.ncbi.nlm.nih.gov/14715260/ DNA methyltransferase (M.EcoP1I) encoded by prophage P1, may protect the phage genome from restriction by type III restriction enzymes and thus could be considered as an anti-restriction system. PF02086.20,PF00145.22,PF02086.20,PF00145.22 MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLAHRWPHVANLGDMTKLAKKVLAGEIESPDVLVWGTPCQAFSIAGLRGGLDDERGALTLKYVELANAIDDKRSESFLKPTVIVWENVPGVLSSADNAFGCFLAGLAGEDAPFEPGDRPESGKSNAFWRWDGKTGCHAPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPATVLFEFEGVRRNIAPRRKKKEIASAIIANGAAISGESLNPCLHADMPPSMKSTKAVNAFRMAAFGEYIDDETASTVKARDFKDATDLAVFSSTGAGFWSEGHGTLRAREQESHEHLVTLAFPERMSGTQHAATKNTSPSLMAKNPTAVCYEVRNAEVAVRRLTPVECERLQGFPDGHTLIPTEKRKKVNSDELAYLRNHYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFTNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGAPNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAHDVLGILL 66412 68676 + 63520 70929 149 754 7.0777 83627.86 6.0 0.52 restriction-modification (RM) CLAN026 APIS107,APIS152 phrog_23296,phrog_23296,phrog_23217,phrog_23217,phrog_38038,phrog_38038,phrog_13621,phrog_13621,phrog_16528,phrog_16528,phrog_8411,phrog_8411,phrog_17284,phrog_17284,phrog_26324,phrog_26324,phrog_363,phrog_363,phrog_56,phrog_56 APIS153 1 antiDnd_p0021 Dnd Piel et al., 2022 https://doi.org/10.1038/s41564-022-01157-1 QZI91758.1 https://www.ncbi.nlm.nih.gov/protein/QZI91758.1 APIS153.hmm Vibrio phage 44E38.1 GCA_020477405.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:35760840 https://pubmed.ncbi.nlm.nih.gov/35760840/ Two genes (p0020 and p0021) provide resistance to the Dnd system (anti-Dnd), p0021 encodes a protein of unknown function. MAQHVKVNPTTDEMLSKLSEKRKRENSFIRTKQDIAAEAIVALYKKEMKNG 26975 27130 - 22038 30849 4 51 10.4077 5877.84 4.5 0.63 Dnd CLAN086 APIS153 APIS154 0 IMGVR_UViG_3300020045_017294|3300020045|Ga0206662_10170475 APIS154.hmm IMGVR_UViG_3300020045_017294 ;;;;;; PF09588.15 MSALVNLEQGSQEWLAFRQDKITATDAVVIMGDSKWKTPYQLYIEKTSQVEEKTRNEAMQRGLDLEPIARELFELKMNVEVFPQVVVKDDWAMASLDGLSPLGNLAVEIKCPGPKDHEIALSGKVPSHYYPQLQHQLYVTDLPKMYYFSFDGDDGVVVTVDRDQKYIDKMVAAEKQFLCCLRDKVPPDLTSDDFILQDSPEWTQIANQWTSVKRLLKKLQDDEENLRDMLIQLAGKSNTKGAGISLCKVVRKGNVDYSKIPELKNVDLERYRKGDSEYWKISEM 127 284 4.8186 32548.19 -7.5 0.77 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS155 0 OK040793_00187 APIS155.hmm OK040793 d__Bacteria;p__Actinomycetota;c__Actinomycetes;o__Micrococcales;f__Microbacteriaceae;g__Microbacterium;s__unclassified Microbacterium species 33882 Bacteria Actinomycetota Actinomycetes Micrococcales Microbacteriaceae Microbacterium unclassified Microbacterium species PF10127.14,PF10127.14 MTEPTTLFKAEWGSRAYGTNTPQSDRDLIQVVIEPPPYVTGLELFNPKHSSTAAEGERSYADDIDTVQYGLQKYAALAIEGNPQVLATLFLTEFIEYNPMFKLLQDERDITVSKQAGRKYLGYMTSQKMRITGEKNRSTNRPELVEIHGWDTKFGMHAVRLGFQGLELMETGKIDLPMQGPALELCRNIRAGKVSKREGLDLIEELQSQLEAAIEKSDFPDRGNRMAMSQILHAIYTHDWTIKNVSI 113275 114018 + 111151 115838 4 247 5.4100 27943.79 -3.5 0.89 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS156 0 IMGVR_UViG_3300045988_166823|3300045988|Ga0495776_111997_99927_100583 APIS156.hmm IMGVR_UViG_3300045988_166823 d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides; 375288 Bacteria Bacteroidota Bacteroidia Bacteroidales Tannerellaceae Parabacteroides PF03374.19,PF08346.17 LTENEDYVVFTEFGENSKGGRPKKEYALTLDAAKELSMVEGNEKGKQARKYFIACEKKLKGENPSYLIADPIKRAEKWIQEEKERQSLKEQTKQLAEENKNLENQIEEDLPKVIFAMAVTESKRSCLVAELAKIICQNGMEVGQNRLFKWLRKKGYLGTKGEYYNQPMQRYIEAGLFEIKKRVITKPNGSTITVSTPMVTPAGQLHILNKFLEYYSKM 27 218 9.3347 25084.92 6.5 0.57 SOS response CLAN047 APIS178 phrog_5817,phrog_377,phrog_14211,phrog_6904,phrog_9438,phrog_15968 APIS157 0 MGV-GENOME-0240670_38 APIS157.hmm MGV-GENOME-0240670 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Succinivibrionaceae;g__Succinivibrio; 83770 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Succinivibrionaceae Succinivibrio PF07728.19,PF00004.34,PF07726.16 MPKLDLTKIFGSKLVVSDLELSTCTIPENIPVKNPSYVFNTATLNPVLLFLAHPFNDCLYINGESGCGKTSLILQIAARLGWGVEQITLSNKCESTDLIGHSTLKKGELVYEYGALTRAMQNGEILLLNEIDLMSPGDLSILNDVLEGKPLTILENNGEVIKPHKNFRVIATANTNGMGDDTGFYSGARTMNQAFMDRFRYMNMSYQPKIEACALISAFPKLEKDTVIKLVQFAKSVRDTINAGVESGVRQISAPFSTRTLLKIAGVVSLNTAYSIQDIVEMCFSLRLPAPEREFIKRTVNDIFGHSKQELKETQKDDNNQKLA* 5 325 6.4887 35861.34 0.0 0.85 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS158 0 MW825358_00150 APIS158.hmm MW825358 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Lysobacterales;f__Lysobacteraceae;g__Xanthomonas;s__unclassified Xanthomonas species 338 Bacteria Proteobacteria Gammaproteobacteria Lysobacterales Lysobacteraceae Xanthomonas unclassified Xanthomonas species PF00293.33,PF01467.31,PF00293.33,PF01467.31 MDTQHDYLVFIGRFEPFHNGHMAVARRALQLGKKLIVLIGSAHKPRSIKNPWIAEERSVMIRTALAEHQDRLIIGQLRDHLYNEDQWIADVQRAVREAVVDFDGRMSFDKQSIRMIGQNKDESSYYLQMFPQWPAIDVSHTAVQSATDLRNYLFDAGRVETHGGMNMIRANVPRPVFEMIDAFRHSSPDFNQLVREHKFIKDYKSGWDTAPYAPTFTTTDAVVTYSGHVLLVRRRAEPGKGLWALPGGFVNPKESIYDSCIRELREETRLRIPASILKGSLKAKEVFDHPDRSLRGRTITHAFHFHFAAGELPPVKGGDDADKARWVPISEVLEMGPRLYEDHLDIIEFFLGQTASQRQSLID 139407 140498 + 135135 145016 153 363 7.4616 41561.30 7.0 0.88 NAD+ reconstitution pathway (NARP) CLAN020 APIS170 APIS159 0 IMGVR_UViG_3300040856_001282|3300040856|Ga0436647_0012686_635_1258 APIS159.hmm IMGVR_UViG_3300040856_001282 ;;;;;; PF07275.16 MRWPIRNALVAFATLTPAESAQRWAFLDVVRHRAQKKGTHMKIYVACLAAYNNGQLHGAWIEASSDVDELQEGVDKVLQSSPQPNAEEYAIHDYDGFPNLGEYPGLERVAEVAGLIETSDFDVDTVKAVINYADDIEGAQKMLDDNHGVHSSFQEYADELADEMMSCHQCKHSDWLKQYFDYEQYARNIKHDYTVIDVPDGVFVAVA 12 207 4.3926 23359.93 -14.5 0.72 restriction-modification (RM) CLAN005 APIS003,ardu APIS160 1 narp1_adps NAD+ reconstitution pathway (NARP) Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 QXV81770.1 https://www.ncbi.nlm.nih.gov/protein/QXV81770.1 APIS160.hmm Escherichia phage JohannRWettstein GCA_020892775.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 Phage enzyme ADPR-PP synthetase (Adps). PF00156.32,PF14572.11,PF00156.32,PF14572.11 MKTVIEAVVTAQEKFFHTEKFNIIQFPSGEIGGNFSEDFVKFTERNAGKIDNVIITVQGYDKDTLFALALAKDAVDSLVPQKSAMKTIVFGFLPNARYDRHMFKGDAAALKVFANLVNAMGFDAVCALDPHSNVAENLFKCFQSMKQKDVAVHFASDPRIDFLVAPDAGAAKKTEDTAKEVDKPYITMSKVRNLKTGEITGMRILDDVDLTDKTVMILDDICDGGRTFVEAAKHLREAGAKRVELYVTHGIFSKGVENLLDNGIDHIYTTNTLGEAKDRGLTHYGQVTVATID 52746 53627 + 51017 58746 157 293 5.8681 32278.87 -3.0 0.89 NAD+ reconstitution pathway (NARP) CLAN027 APIS160 phrog_16932,phrog_16932,phrog_619,phrog_619 APIS161 0 MH160767_00056 APIS161.hmm MH160767 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__unclassified Escherichia species 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia unclassified Escherichia species PF02086.20,PF00145.22,PF14528.11,PF13403.11,PF02086.20,PF00145.22,PF14528.11,PF13403.11 MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALRWPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDYVVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYFKRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIRRWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHFGELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQTCGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKSVGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAIDDKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFWRWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPATVLFEFEGVRRNIAPSRKKKEIASAIIANGAAISGESLNPCLHADMPPGMKSTKAVNAFRMAAFGEYIDDETASTVKARDFKDATDLAVFSSTGAGFWSEGHGTLRAREQESHEHLVTLAFPERMSGTQHAATKNTSPSLMAKNPTAVCYEVRNAEVAVRRLTPVECERLQGFPEGHTLIPTEKRKKVSSDELAYLRNHYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGAPNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAHDVLGILL 57198 60485 + 53736 66055 27 1095 8.3999 121736.85 27.0 0.42 restriction-modification (RM) CLAN026 APIS107,APIS152 phrog_23296,phrog_23296,phrog_23217,phrog_23217,phrog_38038,phrog_38038,phrog_36001,phrog_36001,phrog_16528,phrog_16528,phrog_28563,phrog_28563,phrog_17284,phrog_17284,phrog_26324,phrog_26324,phrog_363,phrog_363,phrog_56,phrog_56 APIS162 0 IMGVR_UViG_3300017791_000355|3300017791|Ga0188906_106978736 APIS162.hmm IMGVR_UViG_3300017791_000355 ;;;;;; VSFQRCHCGHYNDVHDDRVGVCRHVHSNSWCACTKFRGRGDAPEGADHGPEPRPDLKSIAKPGYHLATIAKGELGELSKIQEELDELRDAEAQGVKIMALVEASDLIGAIDAWLAKHHPQTTLADLVLMHEVTRRAFENGRRK 3 143 6.8190 15882.91 2.0 0.55 TIR-STING CLAN019 APIS150 phrog_6408 APIS163 0 IMGVR_UViG_3300005675_000285|3300005675|Ga0074424_100009442 APIS163.hmm IMGVR_UViG_3300005675_000285 ;;;;;; PF10127.14 MTDFYKKPDLLVRTGSHLYGCAVATSDEDTRGLVVPPAEYLLGRKNWEQHETKDPDCVIWNFAKFFNLLERFSPNTAEILFAPQEHIIEITEVGQMMIDNKHLFVSKQLIKPMQGFAFGEWKKAVEYFEQLRKLGAQRKEHIAKFGYSVKNAYHAVRLLEECIELLQTGTITFPRPNADFLRQIRHGEIPVEVVKERYEQLDKRVPQEVANSSIPDSVEKDKLDKLFYDCIKMKMVGFMADNYSLACSDLGLVFSHNHLFNPKWYRVEQPT* 7 272 6.7625 31509.23 1.5 0.82 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS164 1 Lidtsur-17 AVAST (Antiviral STAND) Gao et al., 2022 https://doi.org/10.1126/science.abm4096 YP_009821615.1 https://www.ncbi.nlm.nih.gov/protein/YP_009821615.1 APIS164.hmm Escherichia phage Lidtsur GCF_004800205.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:35951700 https://pubmed.ncbi.nlm.nih.gov/35951700/ Lidtsur-17 inhibits SeAvs3 nuclease activity in vitro. MAINFEKFNKQQLAVVCQLGNEIDATPDVHDIIEFRGGFIPKVVYVAAVRNNRVDSIGIIQPTRTGAVLARNVTLIASLSAKQMELEGDREGVVAMQMAASLAVRFACEETVHDSYHEWFLACEHVPSDLTETQLSLLFAGEVLDQMLHQLQGGLSELMEAARGVKRATLH 5056 5571 + 3666 8664 9 171 5.0008 18833.61 -5.0 0.73 AVAST (Antiviral STAND) CLAN087 APIS164 APIS165 1 Ddra restriction-modification (RM) Piya et al., 2017 https://doi.org/10.1111/mmi.13705 YP_006493.1 https://www.ncbi.nlm.nih.gov/protein/YP_006493.1 APIS165.hmm Escherichia phage P1 GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:28509398 https://pubmed.ncbi.nlm.nih.gov/28509398 It's included in the P1 restriction system. The proteins Hdf, DarA and DdrA are required for the protection of phage DNA from restriction by the EcoA Type I R-M system. MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQMGYGASGDGSGEEKQPSEHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGIYIDLDQVKQHTAAWIAANIKEAA 27542 27907 - 23063 31532 100 121 3.8317 13013.56 -14.0 0.41 restriction-modification (RM) CLAN088 APIS165 phrog_1855,phrog_1855 APIS166 1 vcrx089 restriction-modification (RM) Roy et al., 2020 https://doi.org/10.1093/nar/gkaa518 YP_008997725.1 https://www.ncbi.nlm.nih.gov/protein/YP_008997725.1 APIS166.hmm Vibrio cholerae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:32556263 https://pubmed.ncbi.nlm.nih.gov/32556263/ vcrx089 and vcrx090 promote resistance against type I restriction-modification. PF07728.19,PF08406.15 MSQYSQFSVSKVFGMPSIPEKVTAIGYADGSNPFIPATDTNYVFRKEFLREVLAYLKEPGGDALFVTGPTGSGKTSGITEIAGRLNWPVQQITAHGRMELTDLIGHHALVAEKPGQPPVMKFMYGPLAVAMREGHLFLINEVDLADPAELAGLNDVLEGRPLVIAQNGGEIIKPHPMFRVVVTGNSTGSGDASGLYQGVMMQNLAAMDRYRFTKVGYADEEAELSILGRVTPKLPENVRKGMVRIANQVRKLFLGENGEDGQISVTMSTRTLVRWAKLSLAFRGAPNALEYALDQALLIRAAKEEREAILRVAKDVFGDQWR 7 322 6.9964 35220.51 1.5 0.88 restriction-modification (RM) CLAN004 APIS166 APIS167 0 ON287374_00122 APIS167.hmm ON287374 d__Eukaryota;p__Arthropoda;c__Insecta;o__Coleoptera;f__Lampyridae;g__Serratia;s__unclassified Serratia species 2985502 Eukaryota Arthropoda Insecta Coleoptera Lampyridae Serratia unclassified Serratia species PF08719.16 MKITDKHVLFFSYKDMFSNHFRSERPFFSPRHEREGIKFYTGEHFMMYEKAKLFGDEEISKKILETFHPQEAKKLGRLVKGFNNDVWESHREDIIFSIVYCRLVYDKHLRLSALNHRMDGRSFVEASPWDKIYGIGLKETDPAADDPSQWKGLNLLGKAWDNATDALAEKCGGYELMSKERATWGI 175 186 7.5111 21804.84 4.0 0.91 toxin-antitoxin (TA) CLAN045 APIS125 phrog_1017 APIS168 0 MF319184_00051 APIS168.hmm MF319184 d__Bacteria;p__Actinomycetota;c__Actinomycetes;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium;s__unclassified Mycobacterium species 1763 Bacteria Actinomycetota Actinomycetes Mycobacteriales Mycobacteriaceae Mycobacterium unclassified Mycobacterium species PF10127.14,PF10127.14 MSHNSVWHAQIAHDNLIYKTEVGSVLHGVTLGGQDDNDEMGVCIAPPECVLGTKSFEQYQDRWHADGTRIPEGQRSGPGDTDQTIYSLQKYARLAAKGNPTVLMPLFAPENYVYDCTEEGRELRWNRDLFLSKQVGDRFLGYLVAQRERAQGLRGKKHTNRPELVEKFGYDTKMMYHAHRLAIQGTELLLEGDITLPMEDVHRSFLLRMRNGEFPLEVALKSLEFRTELLRQAAEASELPDHPDYDAIDNWLIGMQYHHWKTKGLL 37068 37868 - 35760 41239 49 266 6.0775 30481.43 -3.0 0.91 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS169 0 IMGVR_UViG_3300044551_000136|3300044551|Ga0455787_00181_52848_53810 APIS169.hmm IMGVR_UViG_3300044551_000136 ;;;;;; PF09588.15 MKRPALRSNDVFDVVRFRQRTRAEREAAWLAERGKGVGGSDMSTILGLNKYQTPYSLWLEKTGRSEHEDISGRWPVIKGNVLEGELRRWFSRRHPEISLTNGTDMSLISLAHPFLRASLDGVIWSEDRGFGVLECKTASAYRAADWHADDGSLKAPDYYMAQVTHYLAVTGWKYGCFVADIGESEPVEVWFERDEDDIKTVVDAAEAFWEFIQRDEPPELTGTDVDELYPQDDGDIELIDSDLFKEMSATYLRLSNQLASLKAQKEKVGQDLKVFIGEHKGLKSGRWQATYTTTHYKESVRKAFDARRLHVSEIKEKNNG 20 320 5.3287 36532.91 -6.0 0.78 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS170 1 narp2_nmnat NAD+ reconstitution pathway (NARP) Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 NP_899408.1 https://www.ncbi.nlm.nih.gov/protein/NP_899408.1 APIS170.hmm Vibrio phage KVP40 GCF_000843785.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 Nicotinamide mononucleotide adenylyltransferase (Nmnat), capable of generating NAD+ by conjugating AMP to NMN. PF00293.33,PF01467.31,PF00293.33,PF01467.31 MSHAIFIGRFRPFHNGHLSAITQAFDALDLDKMTILIGSSNRHRSVKNPFVFEEVRDMMGVALPDHIRSKVRFVPLGDYAKDDVWQSNVRSRARGATHIVGYDKDESSYYLKLFPELKLFQPEPVKMYNKVISATDFRELYFSEILLNHPVMSGLIPKETMMFLDNWSKTEFFTEMKAEYDSSVREIEKFKDYPYQGHLNIACADNVVTCAGHVLLVERKFNPGKGCLALPGGHKHEKETFLDAAIRELQEETNIKVPEKVLRGSLVGEKMFDNPNRSYPHTRITMAYHLKVHPNPDNTFPKVKPADDAVSAKWYPLSEVRDMQERLYDDHYQIIQYFTGI 94507 95532 + 92044 97056 17 341 7.2039 39396.07 5.5 0.88 NAD+ reconstitution pathway (NARP) CLAN020 APIS170 phrog_395,phrog_395 APIS171 0 IMGVR_UViG_3300014656_005885|3300014656|Ga0180007_100018939 APIS171.hmm IMGVR_UViG_3300014656_005885 ;;;;;; PF07728.19,PF00004.34,PF07726.16,PF20030.4 MALDEYQKKYPDEELVSEAFRAKLAELKSKKKLNALTPKKYSIAKTFGLSMGKDSMVTGFVERTQAVPEIDPEYKFHPEATKIVLLGLQTNRPTMVHGPTGSGKSSLVEQIAARINYPVMRVNHHKDMYSYDIVGQKKIEDGTTAFEYGPAPVAMRQPMILIMDEWDATNPEVALLYQSLLERKGDGSRLGNLVLTANGGERVESDPFFRIIATSNTTGLGDDKGYYQGTEVQNIAFVSRFLLRVKLDYMDKKQEGELLKKKFASLSEEEATGFAKVASLIRKRFEAGELNVPYSVRDLINWVDLYLMIGDASKAMQFSCTSILPYTDEKIISEIVQRTFS* 3 342 6.8850 38328.99 1.0 0.81 restriction-modification (RM) CLAN004 APIS166 APIS172 0 IMGVR_UViG_3300033489_000038|3300033489|Ga0299912_1000048148 APIS172.hmm IMGVR_UViG_3300033489_000038 ;;;;;; PF09588.15 MKIINVEQGSAEWHAFRKEHIGGSDAPIIMGVSPYKTRWELWQEKVGISEPKAANSPMRRGSDLEKFVRADIEDRLFCRLIPHVFVHDTIEFLSYSSDGYDEYNDILIEIKCGTRAEHEGVRRGIIPDKYYPQLQHALLVTGMQEIYYVSHNSGETIYSIVERDEAYISKMLVEHRKFHYCMDMEEGPEIDEVHRKSLKKEYITIDNPVFKELAFNWQLLQVSKKNLADREDELKKAIIEYCENENTKGFGITVTKVIKSGYVDYSMIEELKSVDLEKYRKEKGEYWKIECK 3 292 5.6510 34238.01 -5.0 0.67 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS173 0 IMGVR_UViG_3300017971_000595|3300017971|Ga0180438_1000216435 APIS173.hmm IMGVR_UViG_3300017971_000595 ;;;;;; PF09588.15 MQQGTSEWLEFRRSKLGASDAPIVLGISPYKTPHELWLEKTGRKEPPAMNYAMKRGHELEDSLRIAYEELTGNIMMPKVITHDEFDWMMASLDGVSMDGSIILEIKTCNAKIFEAAKSGKPIDHHYAQIQHQLACAPDAEFVHYFIEHKGEHHLLKVVRDEEYIQKLTCDLIMFHQCMIEDIAPELTEDDYVVIEHDPEFAEAAQLWLSAKESLDEAKKAYDTAKSSLIEFTDDGNCMGYGVVLTRCQRKRTDWKQAALDAKVDISQYTSESIGYWTIREVR 3 282 4.8600 32334.73 -12.0 0.76 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS174 0 MZ868713_00032 APIS174.hmm MZ868713 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__unclassified Pseudomonas species 286 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas unclassified Pseudomonas species PF13793.11,PF13793.11 MAWNTGFNENLFYTLNGSRSQRVSVSTMTFPGGEVGVNINTGSVDWKQGYMSNVTRIDLVAKIQNSDQLMAMFLATDALRRVYPLAQIDLLIPYFPYARQDRVCNAGEALSVKVIATLINAQNYATVTVLDPHSAVVVGCLDRCFVTDQIDAFGRIKTDWHNWIIVAPDMGAAKKTEDFAKRVGAKNVLQCNKKRNLADGKILGMEVLNPEILSGKVQLLILDDICDGGRTFTEVAHAIFGIMHADDIDRIELAVTHGIFSKGLNDLTMVYDRIYTTDSLPQDELNNEKLTVMKY 14476 15363 - 10778 18067 51 295 5.6716 32803.65 -2.5 0.88 NAD+ reconstitution pathway (NARP) CLAN027 APIS160 phrog_619,phrog_619 APIS175 0 IMGVR_UViG_3300020393_000012|3300020393|Ga0211618_100001578 APIS175.hmm IMGVR_UViG_3300020393_000012 ;;;;;; PF07728.19,PF00004.34,PF07726.16,PF13401.11 MQYCFADLKSMLKDYKLRDGNESRRILRKFVCDTTHEICSSVRFTKDVEKASGMNWRRYVESVDRCVLLQLIYDSLMGKLDERTYLDPLENPSQHCHPKRRGSGYSFGFNKKIPTNDHGIVRQIETALGDSMTTPPSGKKKTPSIMELSDVICVAEHELKIQSGLLNHEDRLDVTINDAVIHEVCNTIWEGSDYVAPETTESYNQSVIALLRTQTDENGQNVADITYQQKLDGLDEPRKELLSWLRQNYDAYKSSGETSTKQTANVGIEVDQTQLEAINILTKNATQGKYSLTDILNKLDESDKEIKNLKTIKASIKPPLPHGVSHHTGSADDLTYEIVEQKASKVFGKSVKAMQFDIPTLVWKDKSGKEVQHPLCPSIEPNYEFRPQHLVKFLSAHLFGQNIWVHGHTGTGKTTLAEQIASRIGYPVFRLNLDSNMERADIVGAKEIVVENGQPTTRFVEGILPKAMKLPCFLILDEMDGGMPDILFAVQRALEKKGLVLTEDGGRLVESHPYFRFIATANSRGQGDEYGWYQGVRPMNLATLDRFSTFIEVGYLSKDQETKLISKEYPMISNERLGQIVQFTKEIREAFIGGELSTTISPRGVASMCQYFVHMKDLMPDENQAMKSAVDVVITDRCPLDSKQRVTEIAQRCFK 6 655 6.4614 74012.24 -0.5 0.56 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS176 0 MZ666938_00004 APIS176.hmm MZ666938 d__Bacteria;p__Thermodesulfobacteriota;c__Desulfovibrionia;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Desulfovibrio;s__unclassified Desulfovibrio species 872 Bacteria Thermodesulfobacteriota Desulfovibrionia Desulfovibrionales Desulfovibrionaceae Desulfovibrio unclassified Desulfovibrio species PF07275.16 MSEERRIYVASLTDYNSGILHGKWLDLDDFADLEGLQEAVKAMLKESPIMKETGELAEEYVIHDHEGFHGLIGEYTPLAEVWEIHSLLEEHSDNEDALMAYVDTFGGSLADAVGGLDDCYEGEWDSEEAFADNLLEDTGMLSSVPEWAQPYFDVSAFARDLFMTDYVYTDGHVFRNC 60 177 3.7830 19978.92 -29.5 0.84 restriction-modification (RM) CLAN005 APIS003,ardu APIS177 0 IMGVR_UViG_3300032046_000145|3300032046|Ga0315289_1000213119 APIS177.hmm IMGVR_UViG_3300032046_000145 ;;;;;; PF09588.15 MQQGTSQWLMWRRAGLGASDAPVIMGIYTYKTPFQLWEEKLGRECQEKKNQFILDKGHTIEKRARLLYEILNDKPMPPALVEYAKMPFLRASLDGYDAEKNIILEIKYVGKKELQEIRDSGKIPERYYPQVQHQLLVTGAKCVDFFAYNDELDSYTLVKCEPDNDYIHLLVEKEREFWQLVQKQTAPELTEKDYVFIQDQEAQFLIDQWIFKKKLLDKIAEEEQALKKQILEKIKEPRVKFGLVKISRQKRAGNIDYKIIPELQNLDLEKYRKPEIVYWRFDLEKKAE 11 288 7.2144 34259.64 1.5 0.74 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS178 1 AriS SOS response Azulay et al., 2022 https://doi.org/10.1016/j.celrep.2022.110723 WP_014601097.1 https://www.ncbi.nlm.nih.gov/protein/WP_014601097.1 APIS178.hmm prophage of Listeria monocytogenes 10403S GCF_000168695.2 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Listeriaceae;g__Listeria;s__Listeria monocytogenes 1639 Bacteria Firmicutes Bacilli Bacillales Listeriaceae Listeria Listeria monocytogenes PMID:35443160 https://pubmed.ncbi.nlm.nih.gov/35443160/ LMRG_02920 (AriS) encodes in prophage L. monocytogenes strain 10403S. It acts as a RecA inhibitor and inhibits the bacterial SOS response. PF03374.19,PF08346.17 MSNLQVIANDMLPVMENEKGEKFVNARELHQSLQVGKKFATWITDKFNNYGFSKDEDYFPILGESTFGRPRTEYLLTLDTAKELAMVQNNEMGRSIRKYFIEVEKQARKLATEYPTFSYMIEDPVARAKKWIEEQQEKQEALKQLEEQKPKVVFAEAVQTSENTILVKDLATILKQKGLDIGQNRLFEWLRCSGYLLNKGAYYNKPSQKAMNLGLFEQKTHIHTDRNGLMITTYTPRVTGKGQIYLLNKLLEEHNQVII 154 259 8.6275 30067.51 4.0 0.54 SOS response CLAN047 APIS178 phrog_5817,phrog_14211,phrog_6904,phrog_9438,phrog_31873,phrog_15968 APIS179 1 Ddrb restriction-modification (RM) Piya et al., 2017 https://doi.org/10.1111/mmi.13705 YP_006491.1 https://www.ncbi.nlm.nih.gov/protein/YP_006491.1 APIS179.hmm Escherichia phage P1 GCF_000844165.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:28509398 https://pubmed.ncbi.nlm.nih.gov/28509398 It's included in the P1 restriction system. The presence of DdrB results in reduced antirestriction activity, presumably by negatively affecting DarB but the mechanism is not known. PF18763.6,PF18763.6 MSLSDQVVMATSIETLIELLKNLPDFGRVSYVVTAKGDEVKTAFDIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLSSHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIDHATEFGIPPEKVESMTAPVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLLSRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAEEPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKDAVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQRINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESVSGGSYNGVAPYTSLLLHRASGIKDIIHLIRLLSRTDPHDEQLVQVLAHFVRMPVADVKKWCRLFGISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSASSLAEMVQRLSLIFTDHTALGDLTLDEMKDASIQWADQQNEVNSDFLTAFRKAVSKADDARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSLQIVARALADANPKSAAIVNRVIGDIEASEGMGVLKLFLSRAFNPNGNILPGIIGEAKKYVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSYVSEEKALEWADSFITEEVEEVRAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNGVVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPEWCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAASLMNGDGLLELLLGVAKELNNAD 24542 27529 - 21569 30857 285 995 5.4395 108753.15 -13.5 0.49 restriction-modification (RM) CLAN089 APIS179 phrog_1849,phrog_1849,phrog_5143,phrog_5143,phrog_2299,phrog_2299,phrog_14693,phrog_14693 APIS180 1 psib SOS response Petrova et al., 2009 https://doi.org/10.1016/j.molcel.2009.07.026 WP_010892539.1 https://www.ncbi.nlm.nih.gov/protein/WP_010892539.1 APIS180.hmm Escherichia coli d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:19818715 https://pubmed.ncbi.nlm.nih.gov/19818715/ PsiB protein works as an SOS inhibitor that binds to RecA to inhibit its activities. PF06290.16 MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPDNWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVVQVQRRFASEAVSHSLALAASLDTQGYSVNDIIHISMAEGGQV 4 144 4.4865 15852.76 -7.5 0.88 SOS response CLAN090 APIS180 phrog_32496 APIS181 0 IMGVR_UViG_2700989269_000001|2700989269|2701970894 APIS181.hmm IMGVR_UViG_2700989269_000001 d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Carnobacteriaceae;g__Carnobacterium;s__Carnobacterium maltaromaticum 2747 Bacteria Firmicutes Bacilli Lactobacillales Carnobacteriaceae Carnobacterium Carnobacterium maltaromaticum MNSYEVLLDEICSNINVVEAELFNATGLLGVYKEGKGIIIDKNMETDLKKTTLMEEYMHSKYTVGNILDQTSIENRKQELLARKYAYIELVPLEALIEAYSLGLTQYYEVADFLEVDIEFLWDATNYYKSTYGTMFQYKGVLIYFSNTITMEFK 3 154 4.2042 17893.46 -10.5 0.88 SOS response CLAN029 APIS132 APIS182 0 MGV-GENOME-0337501_69 APIS182.hmm MGV-GENOME-0337501 ;;;;;; PF07728.19,PF08406.15,PF00004.34 MTNEVRACYLDMGFVLNGQPNGQKFPGYDKPGDYTPEVRQDYVLPDWAKKYFAQWLRFSARLGALYVTGPSGCGKTSAIRQVAARANWPVYECTAHGRLEMADLIGHLSMAKDGSMSFQYGPLSLAMRDGGLFLLNEIDLLDPSIAVGLNTILDGSPLIIPENGGEIIRPHKRFRFIATANSNGGGDDSGMYTGVLVQNMALIDRFSVIKAGYLDEDTEVSIVSHHKGSIPDDVVRNMVSLAAAVRAANVGTGEGVPVAKTFSTRTLVQWAEWTNAVSSMADPNGPSPLRTGLDIALLNSCSVSDRVAILEMASRIFGEDVMGTADGKSGSGSDDDVLE* 80 340 4.7292 36403.20 -8.5 0.84 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS183 0 IMGVR_UViG_3300025715_000016|3300025715|Ga0209310_1000173107 APIS183.hmm IMGVR_UViG_3300025715_000016 ;;;;;; PF07728.19,PF08406.15,PF07726.16,PF13401.11 MSGSWSDTDRSGNKKVKCEVCGQHFHRLEIHISHEHSMKVADYKAQYPGAPILSEYAKKRASDGQLNRKPTTPKAPQMTIGVDKASGASVTTTSKVFTVGRAQLAQREKAELDPFLDQPYVPAFDDGWEMSDTMKEAWELMALAIDSRQNLLWVGPTGNGKTSSVLQLASLLNQPVQRINMDGDVRKADFVGEKVVDVDETSGQSVTRWVDGILPTAMRRGHWLLIDELDAAPAQILFVLQAVLERSTTGAKLVLTGNGGEVVKAHPNFRILATANTKGRGDDSGMYQGTNSLNEAFLDRFGVVLETSYPDVDVETRILTRKTGIRAEMARKMVSVANEVRRAAANEQCYTTFSTRRLLAWAQQAVWFGAQEINGAAKAVSKAASGAVLNRMSKDDKAFVASLIQRYFGN 3 410 8.9384 44998.03 7.5 0.72 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS184 0 IMGVR_UViG_3300031673_000052|3300031673|Ga0307377_1000042019 APIS184.hmm IMGVR_UViG_3300031673_000052 ;;;;;; PF07728.19,PF08406.15,PF13401.11 MKLTCKVCGHESGNLVPHIENDHPEIPLKSYMDDHGGIEAVIHSSLLAKTKEAKVVKFKKAGVGKVKVAGAEVEKCSCPDELAQHIPETSDAYQFQDFTKDVIYDLNGDVSRPVLLVGHTGCGKTSCFQELAGRLGAPTIRANLNHQTTISDFVGMWGVKGGETYWIDGVLPWAMRNGVWLILDELDFADPAILSVLNSILEPGQPLVLKEKGNEVIKPHKDFRICATANAVGQYAEYRGLYQGTNIMNEAFLDRWRVYIVDYLPEDLERKVLEKSIPLMSAGVAEAMVKVASAVRKAFNEETVQCTFSTRRLLDWGELTIRHRNLKVDAPFKAAESVIFSKISREDALAIRSFMQRILMDRG 3 363 6.6528 40390.50 1.0 0.76 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS185 1 vcrx093 CRISPR-Cas evasion by DNA repair Roy et al., 2024 https://doi.org/10.1093/nar/gkaa518 YP_008997729.1 https://www.ncbi.nlm.nih.gov/protein/YP_008997729.1 APIS185.hmm Vibrio cholerae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:32556263 https://pubmed.ncbi.nlm.nih.gov/32556263/ CRISPR–Cas evasion by repairing double-strand DNA breaks via recombination between short sequence repeats PF09588.15 MKIVNLSQREEDWLDWRRQGVTATDAAILLNRSPYKTRWRLWAEKTGYAREVDLSLNPLVRRGIENEDAARRAFEEKYDDMLLPACVESVQYPLMRASLDGLRDNGEPVELKSPSATVWEDVCAEKANSKAYQLYYPQVQHQLLVTGAKQGWLVFYFEGQIQEFPILRDEAMIQEILAEAKKFWQQVVDKKEPDKDPERDLYIPQGEEVNRWIAAAEEYRLYDAEIQELKQRLSELQERQKPHLDTMKSLMGEYFHADYCGVMVTRYKAAGRVDYKKLLADKASGVKPEDVDQYREKSSERCRVTVTGSVKPRYIVDEDVLAPLDDLPEEVETFYW 10 336 4.6908 39247.38 -12.5 0.72 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS186 0 IMGVR_UViG_3300013126_000333|3300013126|Ga0172367_100037124 APIS186.hmm IMGVR_UViG_3300013126_000333 ;;;;;; PF07728.19 MATIKQSAIAYAEMKLNSVGLSLAAMSIQDVSCLDLSINEANYASGENLGALCQLFTIIATPSPMKVRWAAAQRLITSGLARHNNAVNAVDAIAKRNIPELSKYDNLTYSYVTKNVNLTTMVALAATLSSASTVSCALMSRCDHSTWTAFGDDWGFDEDEYREGVAPILTHRLFGTTGKHLTIEEGCALFPNGPFPNGFKFLNQSSSQPVSSTNQGVSSMNATTANTTVESSSLSTLNAAYHPIINGMLSQSGLNTTIEQIIKDVEAKGELERNLAEIEKKSSAVIEDLRKKLATAAATPAMPSTISVVSNSTTIPNGTINMVSADTIFPLMSGLKLTIPQFTWDHAHPDVPAINPNYIFRKEMLVKALRCLAKGENMWLSGHTGSGKTTFIEQVAARIGWPVARIAFDSNVDRSELVGRMSLSGDGNGGTVSSWLPGILERAVTNGYILLCDEMDAGHPNSLYTLQPLLEGKSLTLLEDGGRIVNASLMFRIAATGNTTGNGDPSGLYPACRILSAATLDRFQTFVNVPYMTQEEEVELISSCSPSLTKKLVTSLAKFAGEMRNAFVSNQTPVSYSPRRSIAFAREVEDLFAMGIGDEGTALSMAFKSKLYDAASEEFRQRITEIAAASLGNIDPDVSIK* 9 642 5.3838 68886.26 -6.5 0.58 restriction-modification (RM) CLAN004 APIS166 APIS187 0 IMGVR_UViG_3300020000_000092|3300020000|Ga0193692_100022025 APIS187.hmm IMGVR_UViG_3300020000_000092 ;;;;;; PF07275.16 MFRVYIACLASYNAGTLYGEWVEISGLTADEIREEIARVLKGSPEPQAEEWAFHDHQGFDGYKMSENPDLEALADHMDAYSSSEYACDLIEGVCDNLGVGAREAIEYIDNNYQGEHKSLEDWADDYLDQSGELSSLPKHLSYYFDYSAYARDCELNGDIFSISTNGGVYVLSNC 4 174 3.8670 19507.24 -22.5 0.83 restriction-modification (RM) CLAN005 APIS003,ardu APIS188 0 IMGVR_UViG_3300017963_006746|3300017963|Ga0180437_1000514718 APIS188.hmm IMGVR_UViG_3300017963_006746 ;;;;;; PF09588.15 MKIITFSEGQGSDSWKKWRSSGIGASDISVIMGSNPYETPLKLWEKKCGYRAEDPLNVAMKHGVENEDIARQWINQNHQLYLKPLCVEDDEEPIFRASLDGWDNDNQVLCEIKCPISEKVLDNARLKQSIPDYWYDQMQWQIMISNPKRAFIALWDYRTQNCITLDMFGSKERIPKMREKAKSFWHNVQIGRAPVPEKSDYIEVQDEELHALLIEYEDLANKCTALSDRKKELKKKIEDYGDDGNFTAYGFKIQRVQPAAKLDVEQMKLDGIDVERYMKKSDSIGYYKIYLPKGNK 13 296 5.8224 34495.30 -2.0 0.81 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS189 0 IMGVR_UViG_3300024259_001623|3300024259|Ga0233437_1000022420 APIS189.hmm IMGVR_UViG_3300024259_001623 ;;;;;; PF10127.14 MGSSIVKKLHDVGLVNPPRFVPINMHYEVITGSVAYGMSGDTSDMDVVGFCIPPKDMVFPHLRGEIEGFGRHKKRFENWQEHHVMHKDKEYDMTVYSIVKFFSLCMEMNPNMVDCLFVPDNCIIHSTQVGQMLRDNRHMFLSKACWPKFKGYAYSQLKKMKNKNPELGSKRAALIDKFGYDTKFASHVVRLIGEVQQILTLGDLNLQQDRERLKAIRRGEWTQEQIEEFFTRMEQELETAYNESKLPWGPDESKIKKLLLECLEHHYGSLEKAISVPGKELQALEEISELCHKALGR 11 297 7.2953 34353.69 5.5 0.88 CBASS,Pycsar,CRISPR–Cas (type III) CLAN010 APIS103 APIS190 0 IMGVR_UViG_3300043908_003701|3300043908|Ga0466286_000809_5255_7327 APIS190.hmm IMGVR_UViG_3300043908_003701 ;;;;;; PF07728.19,PF00004.34,PF07726.16,PF08406.15,PF13401.11 MEKTHKLYQRLRKALSKSFDDMRYDLRKLANEVVETDVSLASHGDALKEIQDNPSMLVDNYSGVKFTWAWIRDRLHTDALIFLCDFGGGVSGGLSASDRFENIMNCIAYPKRSRLNAKSRHGYDLGVPHTASKRIEDFKSELLSDKSTTKQGESMQEERRRSTLDLTLEELMAVFDTVMSHCVSKQVGICEESFVADVMKAFASCYEEENRLIGLQALVKSKLTEGKIPDYTNEIPCEPELDEFVQAILHTLGVEYSYDKQLVKEATTSSIGISKQQQQMVDQLMQSIGASTTIEEMFAETKQAAAHVVEKDEEIAELKKKLSKAQQVKATPAFPTAIVAKTSDATPAGETTNPDDIECEIVKQSAMDIFKSPDGKKIKAFDYEVPVLKWKKPNTDVPEIDPNYVFRGNLLADVLYCILHNQKGFLSGHTGTGKTTLIEQVCARLGYPFKRVNFDSEITRLDLVGREVLHNEGGNTVSKFIDGIIPQAVRQACVLCLDEIDFVRPDVAYVLQRALENKGFTVLEDGDRFIEPNPLFRIFATANTRGQGDETGSYQGARHQSLAFLDRFNVFTHVPYLSEDQEGGLLIRANPTLDEELAKQLVKFAQEVREAFRNGTIYMTVSVRGLLSCASMITYFMPLFENNLNYTLSFAITKSILNRCNAQDFQNISEIAQRVFDTKGGQPLKFKYED 6 690 5.2392 77570.12 -11.5 0.54 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS191 0 IMGVR_UViG_2877594009_000001|2877594009|2877597981 APIS191.hmm IMGVR_UViG_2877594009_000001 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter;s__Acinetobacter baumannii 469 Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Moraxellaceae Acinetobacter Acinetobacter baumannii VSSKSIQYTPEPFRLVRKLNLHLLERNFDPDLYENIVLNHEEIVLTVYLHCLNGKLVGYQQYRPDCNDKRTNNPKLARYFTYKTPGNIAVWGLETLDYSRKRLYIVEGIFKASALHMLGHNAVALLTGSPSIDMLNWLRSLPFELYAIGDNDDTGKRLVRAVGNGTCFEKDVDEYSLEELESLLGSSQI 6 189 6.3349 21627.66 -0.5 0.85 Retron CLAN025 APIS094 phrog_849,phrog_14043 APIS192 0 KM677185_00114 APIS192.hmm KM677185 d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus;s__unclassified Lactococcus species 1357 Bacteria Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus unclassified Lactococcus species PF02086.20,PF02086.20 MDFIKSPLNYTGGKFKLLPKIMPLFYEAETFIDLFGGGGNVGINSNSEKIIINDREKVVIDFFKKIQKLSIEDVLESIKGYIETYDLSKVNQEGFLKIRKDYNTSTEKDPLMFYTMLMYSFNYQIRFNSKGEYNMPFGKDRSSFNKNTEKNIRNFHNAILSKKIIFTNNDFRDIKVDKINSDTMVYCDPPYLITTASYNENGGWTEKEEIDLLNLLDELNDKNIKFALSNVLEHKNKKNTTLIEWSKKYNIHFLDMNYNNSNYQSTANQGKTVEVLITNY 59057 59899 + 52511 64320 118 280 7.2682 32712.20 1.5 0.87 restriction-modification (RM) CLAN026 APIS107,APIS152 APIS193 1 Forsur-7 AVAST (Antiviral STAND) Gao et al., 2022 https://doi.org/10.1126/science.abm4096 QHR71835.1 https://www.ncbi.nlm.nih.gov/protein/QHR71835.1 APIS193.hmm Escherichia phage forsur GCA_010120305.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:35951700 https://pubmed.ncbi.nlm.nih.gov/35951700/ Forsur-7 inhibits SeAvs3 nuclease activity in vitro to a lesser degree. MATINVYKTITGSLVFVVGELREDAPIVGVSLREGATEADAIAEIRQNIENLGGLFFLLQGGSLNLAKLQEHEPVLVTSFEAEDVDASDFEASRADEEEAGE 29791 30099 + 27452 31529 4 102 3.7758 10939.12 -14.5 0.29 AVAST (Antiviral STAND) CLAN031 APIS129,APIS193 APIS194 0 IMGVR_UViG_2802429331_000011|2802429331|2805043655 APIS194.hmm IMGVR_UViG_2802429331_000011 d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Nocardia;s__Nocardia terpenica 1817 Bacteria Actinobacteriota Actinomycetia Mycobacteriales Mycobacteriaceae Nocardia Nocardia terpenica PF07275.16 VTASIYVASLTDYNAGRLHGEWLDVLDFIDGADLLAAVDRMLRKSPTARREGAVAEEWAIHDYDGFGGFEVSAWARFDDVFDLAKTLDELEKLDEAEAFTVFIQEIRGLDEFDGLTDAVAGFRDAYVGAMSPADYAYDVMEDALSQVDDTIRMYFDFEAYGRDLVTGGDMTYAEGFLFWNH 3 181 3.8290 20294.38 -24.5 0.83 restriction-modification (RM) CLAN005 APIS003,ardu APIS195 1 AdfN toxin-antitoxin (TA) Johannesman et al., 2024 https://doi.org/10.1101/2024.07.11.602962 AdfN APIS195.hmm Escherichia phage T4 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia PMID:39026772 https://pubmed.ncbi.nlm.nih.gov/39026772/ Anti-DarT factor NADAR (AdfN), removes ADP-ribose modifications from phage DNA during infection thereby enabling replication in DarTG1-containing bacteria. AdfN, like DarG1, is in the NADAR superfamily of ADP-ribosylglycohydrolases. PF08010.16 MSELEIRSNFRWPSCALSNFAQWPFVMDGIQFGGLEGFLQGCKVKNVEQQRRIFGLSGLAAQQAGRSYARAQDRGTLFWLGVPFSRYSPAWKELYTNAYFEAAIQNKGFRDALQASKGKVLKHSMASGLTKDDTILTEAEFIDVLNLLRDSL 192 152 9.0496 17107.50 3.5 0.85 toxin-antitoxin (TA) CLAN091 APIS195 APIS196 0 IMGVR_UViG_3300000385_000390|3300000385|PR_CR_10_Liq_1_inCRDRAFT_10071824 APIS196.hmm IMGVR_UViG_3300000385_000390 ;;;;;; PF09588.15 MKRIELEQGTQEWLDWRRKRAMASETAAIMGIXPYQSXEQIRAAKRGADXTYTTAAMQRGHDEEPKARYAYEDATGELFEPACFEWEDFGASVDGISMDGEQLLEIKSPVKGRESDRWRVVANGGIDHHDYIQVQHQLMVTDARECFFLVWSGEPDSDEPYVGVTIEPDTTVWDQIKEAWEQFWPTVQAREDDEWREAAEAYREAKKAADKAAQELSEAKQRLIQCAAGSYSYGCGVRVKEISRAGSVDWKRVQKDQLAGVDLEQYRKPGSKFFQVDVTEE* 5 282 4.4831 32161.91 -16.0 0.76 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS197 1 narp1_namat NAD+ reconstitution pathway (NARP) Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 QXV81771.1 https://www.ncbi.nlm.nih.gov/protein/QXV81771.1 APIS197.hmm Escherichia phage JohannRWettstein GCA_020892775.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 561 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Osterman et al., 2024 https://doi.org/10.1101/2024.02.11.579819 Phage enzyme Nicotinamide ADPR-transferase (Namat) PF04095.21,PF18127.6,PF04095.21,PF18127.6 MTKSLYAVPAGLNADAYKSGHVYQYPSATEYLMFNLTPRSDKWFNSPLAIDGVVAFGIQRFVKDYLIDHWNATFFERDKKEAIDEILEVMNGVLGKDAIGREHWEALHDLGYLPVEVYAVEEGTVVPMRVPMIVFQNTVSGFHWVAGYLEDAFSAEIWKACTIATIALHYKRICKKWADLTCDNDLHLPYQCHDFAMRGMSGFTDDAFNAVGHLTSFKGTDSFPAVYTAKRIYGQSYPISDIGSSVPATEHSVMCANIAWEGGNELIEEERRFKGELQTFRRFLTETYPTGIASIVSDTYNFWRTVSEILPALRKEIMERDGKLVIRPDSGDPVHIVTGYKAIHLECAKKAYYEHLSKLEASDTMLDAVLNMKLENISYGIAGWLLSEGYEMVVDREDFEVADTVMLKNAYMVGSANVVTRPVAEIDGAIKTLYNIFGGTTNSKGFKVLDEHIGLIYGDSITLERANEILKRLYEMGFASSNVVFGVGSYTYQYMTRDTFAFAVKATLASIGGKEIMLAKDPKTDSGVKKSAFGGVSPMWDGDKLKAVDGYGFQSFADVLDHPACALRLVFSDSEQFGYTTLGDIRNNIDKQL 53673 55454 + 51308 59932 317 593 5.0539 66249.35 -13.5 0.92 NAD+ reconstitution pathway (NARP) CLAN043 APIS104,APIS197 phrog_15800,phrog_15800,phrog_448,phrog_448,phrog_12872,phrog_12872,phrog_16547,phrog_16547 APIS198 0 IMGVR_UViG_3300050581_000016|3300050581|Ga0531113_000653_18870_19973 APIS198.hmm IMGVR_UViG_3300050581_000016 ;;;;;; PF07728.19,PF08406.15,PF07726.16,PF00004.34,PF04851.20 MKDANFFGKVECRICGAKAHFLEMHLTEVHGMTAADYLKTYPDAKVLSEAAEHKLSQIASDTRNQKVVVKLKDLFGANPFGNPDRATIEAFKAPRPNTPKTDPNYYFNPEILAVVLYAIQNQNMKLLLVGPTGSGKTSIAEQVAARLNRGFYRINFDGDITRADLVGQWVLTVKNEMHFQYGILPKAMREGAVLVLDEWDCVNPSVGMVLQSMIEGKPLTITETGEVIEPHPDFRLIATANTVGQGDDTGLYNGTQPQNFATLDRFTVVEHVDYPTQAKEKKILTQTTGITDDDVLDKLTRTAKLIREAFVKQEIRATMSTRTVVNAARLMLDWGSPKRAYTLGFLNKLTTEDQNVCLEVIQRIWGI 5 367 6.8370 40966.96 1.5 0.78 restriction-modification (RM) CLAN004 APIS166 phrog_249 APIS199 0 IMGVR_UViG_3300009100_000030|3300009100|Ga0075418_1000075726 APIS199.hmm IMGVR_UViG_3300009100_000030 ;;;;;; PF09588.15 METHMKVLSVKQKSEEWLEIRRKCISATDLAPIMGMSAYRSPLMLWEEKMGLRVRETNIAMERGSALEKKAIAWAEGILGCKLYDFTVQSEEYEWAIASLDGISEDGKILVEAKCPGEMVHQRHLSGKINQEYICQTQWQMFVTGCEQCHFESFYGEEGHIIVIPRDNDFIQKMILKASQFLHLLRVQIPPEPTDMDFMPRPDDKWHEAAQLWKMAKKHLKQAEEQEQTYRDILIELSRGYNCKGAGVRTQKVTKKGNIQYGDIPMLKEIDLEFYRKPATSYYMIKDEE* 3 290 6.1108 33665.85 -2.0 0.78 CRISPR-Cas evasion by DNA repair CLAN006 APIS185 APIS200 0 IMGVR_UViG_3300042009_000020|3300042009|Ga0439451_000004_88804_89175 APIS200.hmm IMGVR_UViG_3300042009_000020 ;;;;;; MIERNVDAMVVAIRDWEMDAGESFTDYFLDSWAFGSWAYWLLGKGHTEWANKIININEEKGYVDQEDKFEFFPSYVECDKYGERWVEEEYMKNVRVWAEFLTESDQYFRRVNEFFAGNDEGEE 14 123 3.9584 14837.29 -16.5 0.84 Gasdermin CLAN048 pCARD ArdB 1 ArdB restriction-modification (RM) Belogurov et al., 1993 https://journals.asm.org/doi/10.1128/jb.175.15.4843-4850.1993 AAB36887.1 https://www.ncbi.nlm.nih.gov/protein/AAB36887.1 plasmid pKM101 of E. coli U72482.2 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:8393008 https://pubmed.ncbi.nlm.nih.gov/8393008 Like ArdA, ArdB efficiently inhibits restriction by members of the three known families of type I systems of E. coli and only slightly affects the type II enzyme, EcoRI. PF03230 METIEITARYISENARMNFMPAAFRGAFFSADHFIQSFLNRYAKDYQGGYWEYLQASNGAFFMEAPQPLWLSLPNYFEGECSAREVGIIVCLYAYSYFCGLAYEEGKAELNETMANRYHLLREYVNTLENESQNRIYRAID 141 4.4957 16479.50 -6.0 0.86 restriction-modification (RM) CLAN011 APIS007,ArdB phrog_168,phrog_372,phrog_8308,phrog_5302,phrog_907 ardk 1 ardk restriction-modification (RM) Belogurov et al., 1993 https://doi.org/10.1128/jb.175.15.4843-4850.1993 AAB36885.1 https://www.ncbi.nlm.nih.gov/protein/AAB36885.1 Plasmid pKM101 U72482.2 plasmids 36549 PMID: 8393008 https://pubmed.ncbi.nlm.nih.gov/8393008/ ArdK and ArdR likely serve as ardKR-dependent regulatory system that controls the expression of ArdA and ArdB. PF16509 MAQKNRISETEWKQLLPQMASFAHITTDIGYSVLVKGEKSSDVATRVGRSKQNISSTVKRIWDLYQNTTLKAENGEPLKLVQVWIPASLAETVLKEAAKYSINNITTSEMEKK 113 10.0042 12712.58 4.5 0.58 restriction-modification (RM) CLAN111 ardk phrog_2713,phrog_56,phrog_2606,phrog_8928,phrog_1841 ardr 1 ardr restriction-modification (RM) Belogurov et al., 1993 https://doi.org/10.1128/jb.175.15.4843-4850.1993 AAB36888.1 https://www.ncbi.nlm.nih.gov/protein/AAB36888.1 Plasmid pKM101 U72482.2 plasmids 36549 PMID: 8393008 https://pubmed.ncbi.nlm.nih.gov/8393008/ ArdK and ArdR likely serve as ardKR-dependent regulatory system that controls the expression of ArdA and ArdB. MLATLQHPTVWQLPDRLMLLELLMFDYRNSDQERYGQQIYHHYRKQGNHRWDTSVHQDSGGQYAIIFRHSFSKKQADGVKRTMIRDETVIRAGTAQELTEATFPDFQDSDILKASDFFKSLIQRKAADVTQTDI 134 7.0735 15705.64 2.0 0.69 restriction-modification (RM) CLAN112 ardr phrog_381,phrog_3285,phrog_790,phrog_2083,phrog_6102 ardu 1 ardu restriction-modification (RM) Meima & Lidstrom, 2000 https://doi.org/10.1128/AEM.66.9.3856-3867.2000 AAF44049.1 https://www.ncbi.nlm.nih.gov/protein/AAF44049.1 Shuttle vector pI3 AF206717.1 vectors 29278 PMID:10966401 https://pubmed.ncbi.nlm.nih.gov/10966401/ The gene was from contructed E. coli-D. radiodurans shuttle vector,pI3, and trikingly similar to the known plasmid-encoded antirestriction proteins like ArdA. PF07275 MTYTHPLIIERHPDAPALWIGCLAAYNAGKLHGAWMQASSDTAEMFGAIEEILKASPEPHAEEWDIMDTDNMPTEAGRTLDSAATYVAALDALSRADAAEIVAAWVEWRGAEEMDADKITDAYLGRFDSVEDYAAQYLDDSGALQEVPEWLRPYINTAALGRDMEINGDVYEGKNGHFFNGHA 183 4.0134 20139.24 -20.0 0.87 restriction-modification (RM) CLAN005 APIS003,ardu phrog_2,phrog_305,phrog_3336,phrog_1980,phrog_453 gp54 1 gp54 superinfection exclusion Dedrick et al., 2017 https://www.nature.com/articles/nmicrobiol2016251 YP_001469287.1 https://www.ncbi.nlm.nih.gov/protein/YP_001469287.1 Mycobacterium phage Tweety GCF_000871965.1 d__Bacteria;p__Actinomycetota;c__Actinomycetes;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium; 1763 Bacteria Actinomycetota Actinomycetes Mycobacteriales Mycobacteriaceae Mycobacterium PMID:28067906 https://pubmed.ncbi.nlm.nih.gov/28067906 A tetrapeptide repeat protein that acts as a highly effective counter-defense system by preventing activation of (p)ppGpp synthesis encoded by Phrann prophage-mediated defense system. MSIDLDRITHPLRLAKGSHQPGSGKGCAMNVISYINGDTKITDYPECSARPLAALVQMCNDQLAGPDGFLSAENSVLVLDLGWQTVGTAGVSDAVHALWIADMLDSPEWGVIRFADEVGAVAIREIADLHRQAAAGQVPFAWAAWSAAGYAAWSAAWSAAQSAAGYAAGSAAGYAAGSAAGSAAGSAAGYAAGSAAGSAAGSAAWSAAWYAAGSAAWYAAGSAAQSAAGYAAGSAAGSAAGSAAGSAAGSAAGYAAGSAAWYAAGSAAWYAAGSAAQSAAGSAAGSAAGSAAGSAAGSAAWSAAGSAAGSAAGSAAWSAAGSAAWSAAGSAAGSAALIEFTRQAIARWRELAGLDLETEIDAADINAALARING 38644 39768 + 36917 41732 374 4.3748 35599.87 -9.0 0.53 superinfection exclusion CLAN114 gp54 phrog_2335,phrog_372,phrog_2662,phrog_1922,phrog_8841 pCARD 1 pCARD Gasdermin Wein et al., 2023 https://doi.org/10.1101/2023.05.28.542683 YP_004300585.1 https://www.ncbi.nlm.nih.gov/protein/YP_004300585.1 Acinetobacter phage 133 GCF_000891695.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Moraxellales;f__Moraxellaceae;g__Acinetobacter; 469 Bacteria Proteobacteria Gammaproteobacteria Moraxellales Moraxellaceae Acinetobacter PMID:37398489 https://pubmed.ncbi.nlm.nih.gov/37398489/ A phage CARD-only (pCARD) protein with a predicted CARD-like structure that can inhibit the CARD-containing bacterial gasdermin system. MIKVDRENIDAFAARIKNFELKAGESFTDYFMDSEVFAGSWGFWLIGKGYVERGNAIINAYNKANKKHWSDQELCFAVNASPLRWDAASNEFYPWGLDQGINLDLAINADYKLWAEFLISSDRYYEPFLKYLENFEAGGEY 2656 3081 - 1 6642 141 4.3573 16328.24 -7.5 0.78 Gasdermin CLAN048 pCARD phrog_4211,phrog_6005,phrog_1293,phrog_9998,phrog_33 psia 1 psia SOS response Samuel and Burstein, 2023 https://www.biorxiv.org/content/10.1101/2023.02.15.528439v1 WP_042934779.1 https://www.ncbi.nlm.nih.gov/protein/WP_042934779.1 Enterobacteriaceae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia; 543 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Samuel and Burstein, 2023 https://www.biorxiv.org/content/10.1101/2023.02.15.528439v1 SOS-inhibitor. PF06952.16 MISSMLVPINPFQRCAMASIASVENRRALGRKTGELAYVKAFFKELTGKSQINSNDLRKVDSTYDPFVRGEATKVEYLRAIDILIESRGECFILPLSWSLAVSMFPELQNRVFQRREHRDRIHDQRVERLRKKESNLLNYQFEINVAKSRLYLSFCVPGQHKRWLEDWRNDTHGQGLTTNNIYLLIKEWWSSFWITSYRKDYIWCYTLAELLDEIDYVLSTITIMEFNVCFSALPLSLIYKAG 243 8.8190 28599.95 7.0 0.52 SOS response CLAN117 psia phrog_1210,phrog_5493,phrog_469,phrog_6616,phrog_2885 Stp 1 Stp restriction-modification (RM) Penner et al., 1995 https://www.sciencedirect.com/science/article/pii/S0022283685703439 NP_049878.1 https://www.ncbi.nlm.nih.gov/protein/NP_049878.1 T4 of E. coli GCF_000836945.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:7791212 https://pubmed.ncbi.nlm.nih.gov/7791212 Stp has been implicated with activation of the anticodon nuclease and inhibition of EcoprrI restriction. PF08133 MSNFHNEHVMQFYRNNLKTKGVFGRQ 165505 165585 - 162967 166628 26 10.8827 3183.62 4.0 0.28 restriction-modification (RM) CLAN107 Stp phrog_4998,phrog_1138,phrog_2439,phrog_5185,phrog_791 vcrx090 1 vcrx090 restriction-modification (RM) Roy et al., 2021 https://doi.org/10.1093/nar/gkaa518 YP_008997726.1 https://www.ncbi.nlm.nih.gov/protein/YP_008997726.1 Vibrio cholerae d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:32556263 https://pubmed.ncbi.nlm.nih.gov/32556263/ vcrx089 and vcrx090 promote resistance against type I restriction-modification. MKKNDCLCRRYTFKDALTSETFEVFIGYKLLREPSSSGPGQFTMVKLNRTVTDGKAENWSETKLEGPFEANGPDTIPMSYKDKESQYVSQFLSQGYTFLDEVLVNAETQTVLEGGSVSAGQTASLGSLNWLLSPPSELPPGDINLFKGFVAGVFAKGAGLIGFEVARSEGSNDLLPSVLMRTDSGYELGVSTGLGENTIHPATLEGAGELRPEHGHKPLLMLVYLQQRFADDFSNVEKPLVAFCDEQGDTFDYERFDSLKPLIERFGFSYDEVRADAERLGLVSELIRLAEIDAEQEDHFF 301 4.2908 33287.28 -19.0 0.59 restriction-modification (RM) CLAN119 vcrx090 phrog_1330,phrog_58,phrog_6128,phrog_23,phrog_6468 ADG.17 1 ADG.17 toxin-antitoxin (TA) Bhoobalan-Chitty et al., 2024 https://doi.org/10.1038/s41467-024-48074-x YP_009008111.1 https://www.ncbi.nlm.nih.gov/protein/YP_009008111.1 Sulfolobus monocaudavirus SMV1 GCF_000917075.1 d__Archeae;;;;;; 2157 Archeae PMID:38698035 https://pubmed.ncbi.nlm.nih.gov/38698035/ ADG.17 and its homologs in archaea viruses act as antitoxins to inhibit host toxin Doc. It's the first example of an inhibitor of an archaeal toxin-antitoxin immune system. MKCEDIINSMVQYCAIFNREFEMVATHKLTDEQKKILERMHNRVDYILETYKEYLDALAEFDRTGVLKIHGKVIYVKENKNQG 44113 44364 - 40775 47811 83 6.7102 9901.44 0.5 0.54 toxin-antitoxin (TA) CLAN055 ADG.17 phrog_1062,phrog_80,phrog_645,phrog_7376,phrog_2448 anti_TerI2 1 anti_TerI2 TerI Azulay et al., 2025 https://www.biorxiv.org/content/10.1101/2025.02.27.640495v2 AEO07300.1 https://www.ncbi.nlm.nih.gov/protein/AEO07300.1 Listeria monocytogenes 10403S GCF_000168695.2 d_Bacteria;p__Bacillota;c__Bacilli;o__Bacillales;f__Listeriaceae;g__Listeria;s__Listeria monocytogenes 1639 Bacteria Bacillota Bacilli Bacillales Listeriaceae Listeria Listeria monocytogenes Azulay et al., 2025 https://www.biorxiv.org/content/10.1101/2025.02.27.640495v2 Anti-TerI2 counteract TerI during prophage induction to allow virion production. MIFTVNSFPQSGHEFTPGLTVNTCEHSGQVPSVPFLIISSLAIFRLCSLICLSNFFESNLILTPILPTSLHKNYSTVKGRTERRTKCQIYK 91 8.9084 10220.92 5.5 0.28 TerI CLAN110 anti_TerI2 phrog_1506,phrog_6435,phrog_4033,phrog_3830,phrog_1107 APIS265 1 anti_TerI1 TerI Azulay et al., 2025 https://www.biorxiv.org/content/10.1101/2025.02.27.640495v2 AEO07301.1 https://www.ncbi.nlm.nih.gov/protein/AEO07301.1 APIS265.hmm Listeria monocytogenes 10403S GCF_000168695.2 d_Bacteria;p__Bacillota;c__Bacilli;o__Bacillales;f__Listeriaceae;g__Listeria;s__Listeria monocytogenes 1639 Bacteria Bacillota Bacilli Bacillales Listeriaceae Listeria Listeria monocytogenes Azulay et al., 2025 https://www.biorxiv.org/content/10.1101/2025.02.27.640495v2 Anti-TerI1 counteract TerI during prophage induction to allow virion production. MNNIKQAIIKLETILENGNAIESGSFVKYSVIKNILNLLEKDQELKIIEMEVELNGVEDSIENAALLEKRLSEAKSLVEDLASTINSLEIKVK 27 93 4.3472 10385.00 -6.0 0.33 TerI CLAN053 APIS265 phrog_3869,phrog_6651,phrog_9148,phrog_1218,phrog_5181 APIS276 1 T7K Retron-Eco9,DarTG1 Bartolec et al., 2024 https://www.biorxiv.org/content/10.1101/2024.12.20.629319v1 NP_041959.1 https://www.ncbi.nlm.nih.gov/protein/NP_041959.1 APIS276.hmm Escherichia phage T7 GCF_000844825.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Bartolec et al., 2024 https://www.biorxiv.org/content/10.1101/2024.12.20.629319v1 The T7 kinase weakens the activity of phage defense systems by phosphorylation, and is likely a broadly-acting phage counter-defense system. PF27024 MNITDIMNAIDAIKALPICELDKRQGMLIDLLVEMVNSETCDGELTELNQALEHQDWWTTLKCLTADAGFKMLGNGHFSAAYSHPLLPNRVIKVGFKKEDSGAAYTAFCRMYQGRPGIPNVYDVQRHAGCYTVVLDALKDCERFNNDAHYKYAEIASDIIDCNSDEHDELTGWDGEFVETCKLIRKFFEGIASFDMHSGNIMFSNGDVPYITDPVSFSQKKDGGAFSIDPEELIKEVEEVARQKEIDRAKARKERHEGRLEARRFKRRNRKARKAHKAKRERMLAAWRWAERQERRNHEVAVDVLGRTNNAMLWVNMFSGDFKALEERIALHWRNADRMAIANGLTLNIDKQLDAMLMG 2021 3100 + 1278 7763 479 359 6.5721 41123.79 0.5 0.6 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_2206,phrog_1067,phrog_964,phrog_5493,phrog_3779 APIS224 1 acb4 cyclic oligonucleotide-based antiphage signalling system (CBASS) Chang et al., 2025 https://www.cell.com/molecular-cell/fulltext/S1097-2765(00617-3 YP_002300322.1 https://www.ncbi.nlm.nih.gov/protein/YP_002300322.1 APIS224.hmm Bacillus phage SPO1 GCF_000881675.1 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus; 1386 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus PMID:40845805 https://pubmed.ncbi.nlm.nih.gov/40845805/ 9E4W Acb4 proteins bind nucleotide signals and inhibit a broad range of CBASS operons. PF13876, PF13876 MKINAENFECLRESKLKRKVYEDLVKEATFVRVSPKSTVCVVTDHNSFEVIGTSSVYKVENFNDEIGRDTALSQALDSFIKFLAYSGELSDVLENI 19399 19689 + 17705 21757 6 96 4.6794 10871.30 -3.5 0.88 cyclic oligonucleotide-based antiphage signalling system (CBASS) CLAN093 APIS224 phrog_4492,phrog_687,phrog_4211,phrog_1041,phrog_1218 APIS293 1 ZadI-1 Zorya type I Costa et al., 2025 https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(00239-2 QGJ86961.1 https://www.ncbi.nlm.nih.gov/protein/QGJ86961.1 APIS293.hmm Pseudomonas phage vB_PaeM_SMS12 GCA_009685865.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:40639337 https://pubmed.ncbi.nlm.nih.gov/40639337/ ZadI-1 inhibits Zorya type I defense, as shown by efficiency of plating, culture collapse,and phage propagation assays MTSSKWTIGRNDTIEVEAVNSREDFRWNGKIRVIHYSAGQIVNIIEFYHHDLDWAIKNFGIKLKAVSKGLEILHTCYFGKYVK 65118 65369 - 63219 66333 51 83 9.4662 9701.15 5.0 0.57 Zorya type I CLAN097 APIS293 phrog_329,phrog_2373,phrog_3493,phrog_2158,phrog_4200 APIS297 1 Bdi1 Zorya type I,RADAR,Hypnos,Druantia type I Costa et al., 2025 https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(00239-2 YP_002456012.1 https://www.ncbi.nlm.nih.gov/protein/YP_002456012.1 APIS297.hmm Pseudomonas phage PB1 GCF_000883535.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:40639337 https://pubmed.ncbi.nlm.nih.gov/40639337/ Bdi1 and Bdi2 function as multipurpose anti-defense proteins capable of inhibiting four defense systems. MAKVVRVDELKTGDEILIKLRADAAARNKAIVLSVECWRDEITLELTCPAGDYWEDWRGKYRAYDKVVLLKRD 62042 62263 - 56801 64020 42 73 6.2960 8482.83 0.0 0.38 Zorya type I,RADAR,Hypnos,Druantia type I CLAN098 APIS297 phrog_6651,phrog_7507,phrog_601,phrog_384,phrog_8420 TadIII-1 1 TadIII-1 Thoeris type III Costa et al., 2025 https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(00239-2 BBJ26915.1 https://www.ncbi.nlm.nih.gov/protein/BBJ26915.1 Pseudomonas phage S50 GCA_005892745.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:40639337 https://pubmed.ncbi.nlm.nih.gov/40639337/ TadIII-1 inhibits the anti-phage activity of Thoeris type III by a yet unknown mechanism MFRNFFIQTLYFHSDYRHNLLIETRNPENKTMNATYQALKTLRDSCEAAKDEKGTINGNKLNALRNKAVKEMEAGGETYSDAIAMAHDLIKKYRKQSPARFAGP 13254 13568 - 11677 16435 104 9.8948 11930.55 6.5 0.56 Thoeris type III CLAN108 TadIII-1 phrog_1497,phrog_81,phrog_3869,phrog_3956,phrog_453 DadIII-1 1 DadIII-1 Druantia type III Costa et al., 2025 https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(00239-2 WEC87855.1 https://www.ncbi.nlm.nih.gov/protein/WEC87855.1 Pseudomonas phage PA_LZ7 GCA_029207585.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:40639337 https://pubmed.ncbi.nlm.nih.gov/40639337/ DadIII-1 inhibits Druantia type III, most likely through a mechanism specifically targeting the activity of DruH, a protein unique to this Druantia type MPSSSKRVDPSFIRESLRLLCGLFGPREPADGRKDQDRTLNSLGSGAAVLWAIAMSIEVYPVVVAAPELDRSGSSLEHHRAFVSKNMFRHRMFLYNVQLCFTSAQEEYTIS 52610 52945 + 48590 56560 111 7.8847 12459.26 2.5 0.33 Druantia type III CLAN105 DadIII-1 phrog_1506,phrog_4219,phrog_345,phrog_1497,phrog_1698 Bdi2 1 Bdi2 Zorya type I,RADAR,Hypnos,Druantia type I Costa et al., 2025 https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(00239-2 YP_006200849.1 https://www.ncbi.nlm.nih.gov/protein/YP_006200849.1 Pseudomonas phage JG024 GCF_000900635.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:40639337 https://pubmed.ncbi.nlm.nih.gov/40639337/ Bdi1 and Bdi2 function as multipurpose anti-defense proteins capable of inhibiting four defense systems. MMAKILMACELLVGDEILTHVDVNDPVRRRAIVLRSGAAPHSKVSIEVSALIGADWVDFKLKLAGTILPSAYMTSTRSALAALPNRRTPWQKL 62791 63072 - 59977 64837 93 10.3558 10168.04 5.0 0.38 Zorya type I,RADAR,Hypnos,Druantia type I CLAN104 Bdi2 phrog_3904,phrog_4271,phrog_388,phrog_6435,phrog_2439 APIS211 1 SequestinJ Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1761 Ga0376351_0297592_58849_59067 APIS211.hmm IMGVR_UViG_3300037191_001756 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Thoeris anti-defense sponge; sequesters 3′cADPR and blocks ThsA activation PF21825 MKDTFKERLLIETQDLAEKINKLNTFMATKMFMVLNRKDKDLLYEQQRTMSVYLQILGKRLERLDIKFEHKD 58849 59067 - 50951 61731 47 72 9.9287 8796.38 3.5 0.78 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1775,phrog_4201,phrog_4440,phrog_8917,phrog_2263 APIS245 1 SequestinM Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1762 Ga0495776_136213_37340_37531 APIS245.hmm IMGVR_UViG_3300045988_171361 d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides; 408170 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Sequestin-family anti-defense protein; inhibits type I Thoeris signaling, most likely by binding 3′cADPR. PF21825 MSDFKSRLIEEQAQLEEKLNKLNDFNQSEKVNAIDPVQKSLLLVQAGAMYTYNECLKERLARL 37340 37531 - 35467 40532 58 63 5.0180 7316.39 -1.0 0.79 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4211,phrog_4120,phrog_2598,phrog_2120,phrog_10871 APIS283 1 SequestinD Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1763 F14TC_1004933452 APIS283.hmm IMGVR_UViG_3300000559_000188 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Thoeris anti-defense sponge; sequesters 3′cADPR and blocks ThsA activation PF21825 MKPHEERVVIEKKELDAKRERLAAFFSSGVFPALDKAERDQLHRQLEAMDLYSKILGERIKGFPP 92 65 8.6394 7566.77 2.0 0.81 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4201,phrog_1098,phrog_1716,phrog_3856,phrog_6278 APIS283 1 SequestinE Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1764 Ga0206652_10024357 APIS283.hmm IMGVR_UViG_3300020049_004214 freshwater metagenome 449393 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Thoeris anti-defense sponge; sequesters 3′cADPR and blocks ThsA activation PF21825 MEKELLPYQQRVVDERQELETKICALSTFLEGVVFSTLSQDEQDRLIAQVYIMQAYSSILQSRIKNF 4557 4760 + 1146 9619 92 67 4.4384 7872.02 -3.0 0.78 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_5218,phrog_9582,phrog_8735,phrog_8323,phrog_7257 APIS287 1 Acb5A Type I CBASS Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1765 DTR_461702_26 APIS287.hmm d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ cleaves cyclic guanosine monophosphate–adenosine monosphosphate (3′3′-cGaMP) and related molecules PF26761 MNLKTFRLVRTEDVSGVSGTGTVAMGVVFPDGHAAMRWVVGEHRSTVVWDSVESIEAVHGHGGRTTIQWDEA 92 72 6.0525 7821.76 -1.0 0.8 Type I CBASS CLAN002 APIS287,APIS361 phrog_864,phrog_4492,phrog_4406,phrog_2249,phrog_777 APIS288 1 LockinE Type II Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1766 Ga0123573_1000004233 APIS288.hmm sediment metagenome 749907 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Lockin-family type II Thoeris inhibitor; expected to neutralize the type II Thoeris signal His-ADPR. PF26760 MEEKTLDISDQKGARENISDIQIVGNTDMFQLLCKASSKEQGWMKSTKAMEITGRECIVGGCVVQVTTQQKNPDGSYSIAEALTFVPGAKIIPDENGGRRLVSI 23859 24173 + 18882 26998 247 104 4.8144 11306.90 -2.0 0.8 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_6090,phrog_1497,phrog_1068,phrog_8147,phrog_442 APIS288 1 LockinA Type I Thoeris,Type II Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1767 Ga0172381_1000022295 APIS288.hmm B. subtilis phage SBSphiJ IMGVR_UViG_3300014204_000177 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ act as nucleotide “sponges,” binding 1′′–3′ glycocyclic adenosine diphosphate–ribose (3′caDPr) and histidine conjugated to aDPr (his- aDPr) PF26760 MKEKDLGITEVRGAKANITDLVVYGNGDTFALLCKASSQEQGWMKSTKVCNVYGGCIVQVTTQQRNPDGSYALAEALTFVPNNHIDTSGNTRFIGKI 63416 63709 - 61874 69760 247 97 7.7130 10499.91 1.5 0.8 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_4406,phrog_1041,phrog_4440,phrog_4352,phrog_3142 APIS288 1 LockinD Type I Thoeris,Type II Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1768 Ga0316602_100006824 APIS288.hmm wetland metagenome 1325974 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Lockin-family anti-defense sponge active against both type I and type II Thoeris; likely neutralizes 3′cADPR and His-ADPR immune signals. PF26760 MKEKDLNITEVRGAKANISDLQVYGDGDTFALLCKASSQEQGWMKSTKVANVPGGCIVQITTQQKNPDGSYAVAEALTYVPSVMLDKEANPRKLVPTCFCDKVCHV 3759 4079 - 1 7266 247 106 6.8771 11509.21 0.5 0.76 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_7507,phrog_1218,phrog_4795,phrog_1885,phrog_4585 APIS315 1 SequestinK Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1769 Ga0206646_100012446 APIS315.hmm IMGVR_UViG_3300020046_001482 freshwater metagenome 449393 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Sequestin-family Thoeris inhibitor; anti-defense protein that blocks the 3′cADPR → ThsA signaling pathway. PF21825 MIKYRPYQQRVVDEAAELFLKLDSLTKFKTTPSYTKLSLVDQNLLQEQWMSMTDYLDILQLRIDRFKLETPHNP 22632 22856 + 20478 24494 192 74 7.0069 8907.32 0.5 0.77 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3287,phrog_8147,phrog_453,phrog_2197,phrog_2331 APIS315 1 SequestinB Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1770 Ga0209916_1000062263 APIS315.hmm IMGVR_UViG_3300024996_000004 wastewater metagenome 527639 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Anti-Thoeris Sequestin-family sponge; neutralizes Thoeris signaling by trapping 3′cADPR and blocking downstream NAD-depletion defense. PF21825 MQDFQQRVIDEKSELDNKIDKLATFVDSDKFETLSAKDRSLLYQQLNIMINYSAILGARIAGFEI 214880 215077 + 211705 219302 192 65 4.4295 7499.53 -3.0 0.82 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_453,phrog_973,phrog_1716,phrog_2729,phrog_3971 APIS315 1 SequestinL Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1771 Ga0376666_0017886_1340_1546 APIS315.hmm IMGVR_UViG_3300036828_001215 soil metagenome 410658 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Viral anti-Thoeris sponge; binds/sequesters ADPR-derived Thoeris signal molecules and prevents growth-arrest/cell-death defense. PF21825 MQPHEERVIAEELELSAKLDRLEDFVHGPMFVPLPVEDRKLLIEQTAAMALYADVLKRRIARFDGSGV 1340 1546 - 1 5877 192 68 4.7769 7762.00 -3.0 0.79 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2376,phrog_1366,phrog_79,phrog_3245,phrog_2120 APIS315 1 SequestinH Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1772 Ga0415385_0000678_58054_58278 APIS315.hmm IMGVR_UViG_3300042691_027491 fungus metagenome 1670606 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Phage Sequestin homolog; functions as a Thoeris inhibitor by preventing immune-signal activation of the effector. PF21825 MTMAATEEKAELQPYQQRVLEEQKDLDAKIEKLSQFLEKGPTHTIKSEELNRLRLQYQAMCLYSAILGQRIAAF 192 74 5.8229 8586.90 -0.5 0.72 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1802,phrog_7507,phrog_206,phrog_2598,phrog_3011 APIS315 1 SequestinF Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1773 Ga0467023_0000079_5431_5631 APIS315.hmm IMGVR_UViG_3300045257_000280 soil metagenome 410658 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Sequestin-family viral anti-defense protein; inhibits Thoeris by binding the TIR-produced ADPR-derived immune signal. PF21825 MQPHQQRVVDEKKDLDEKINKLKAFIETNPTFKTLPDDERRRLGRQFDAMAEYSSILSQRIAAFPA 192 66 8.6383 7733.80 1.5 0.82 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3285,phrog_4201,phrog_6005,phrog_8147,phrog_1497 APIS356 1 SequestinI Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1774 Ga0453769_004768_81_302 APIS356.hmm IMGVR_UViG_3300042019_000146 estuary metagenome 1649191 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Sequestin-family antidefense protein; neutralizes Thoeris signaling molecules and allows phage escape from Thoeris defense. PF21825 MRHGDMSQHIERMKIEHNELKVKLEALNCFIHKNEIFKDLDHDEQIRMIQQAGFMKSYLDVLNSRLWVAHGNK 81 302 + 81 3169 35 73 7.4755 8760.13 3.0 0.79 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_252,phrog_1858,phrog_9148,phrog_2729,phrog_1770 APIS359 1 LockinB Type I Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1775 Ga0196963_1000032438 APIS359.hmm IMGVR_UViG_3300020215_000018 soil metagenome 410658 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Lockin-family type I Thoeris inhibitor; sponge protein that sequesters 3′cADPR, preventing Thoeris effector activation. PF26760 MTVEKTLTNTTASQAKDNVSDLKVWGDGDMFQLLCKASSQAEGWMKSTKAMNIENGGVVVQVTTQQRNPDGSYSLAEAVTYVPNARVVTSATDDAGNVTGRKIVGYVDLIDP 17597 17935 + 15371 19838 13 112 4.5105 11972.39 -3.0 0.8 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1775,phrog_345,phrog_9228,phrog_2331,phrog_4250 APIS361 1 Acb5B Type I CBASS Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1776 Ga0247671_100000416 APIS361.hmm IMGVR_UViG_3300024284_000007 soil metagenome 410658 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ cleaves cyclic guanosine monophosphate–adenosine monosphosphate (3′3′-cGaMP) and related molecules PF26761 MRRFKVYRPNPPEGYLESGTANPPEEVQFEGVVFSDGTVCVRWLTEFRSHSLWSSLADLVKVHGHSEYGTLWEWLDE 5727 5960 - 4373 10159 18 77 4.7571 8956.98 -3.5 0.85 Type I CBASS CLAN002 APIS287,APIS361 phrog_3904,phrog_3333,phrog_10477,phrog_4211,phrog_148 APIS372 1 SequestinC Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1777 Ga0498813_000005_8406_8615 APIS372.hmm IMGVR_UViG_3300049028_000005 rhizosphere metagenome 939928 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Small phage anti-defense protein from the Sequestin family; inhibits type I Thoeris by interfering with 3′cADPR-mediated immune activation. PF21825 MDLKPHQQRVVTEKAELDERLGKLLAFFQQPIFAGLPEAERSRLRNQARFMDGYSAVLGERIDAFSGKA 8406 8615 + 5220 11655 122 69 8.6831 7848.99 1.5 0.78 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4406,phrog_8689,phrog_9228,phrog_5313,phrog_6819 APIS373 1 SequestinG Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1778 Ga0116171_100188554 APIS373.hmm IMGVR_UViG_3300009692_000337 activated sludge mtagenome 942017 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Anti-Thoeris sponge protein; blocks Thoeris immune signaling, likely through sequestration of 3′cADPR. PF21825 MINKAIEQAQKVGIDRLGFQQRVVDEKAELDEKITKLAAFIETFSAPFSVFGALPEPERYRLYAQHRAMVAYSAILGERIAAFGGVR 3519 3782 + 14 5140 3 87 8.5047 9744.26 1.5 0.72 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3287,phrog_4171,phrog_6518,phrog_247,phrog_9228 APIS378 1 SequestinA Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1779 Ga0495776_150969_77600_77863 APIS378.hmm IMGVR_UViG_3300045988_056527 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Phage-encoded anti-Thoeris sponge protein; binds/sequesters the type I Thoeris immune signal 3′cADPR, preventing activation of the ThsA NADase effector. PF21825 MEKSVFDRLLTEYKELETKTTKLRDFLINKIDKTSIDNLNKDLLIAQLKAMEAYLTILSIRIGLNQPTQEEKQLDEAKALAKSTINE 77600 77863 - 73791 81107 83 87 5.1851 10041.63 -1.0 0.78 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1497,phrog_5302,phrog_7507,phrog_10477,phrog_8663 LockinC 1 LockinC Type I Thoeris Tal et al., 2026 https://www-science-org.libproxy.unl.edu/doi/10.1126/science.aea1780 Ga0115026_1000000314 IMGVR_UViG_3300009111_000005 wetland metagenome 1325974 PMID:41785351 https://pubmed-ncbi-nlm-nih-gov.libproxy.unl.edu/41785351/ Lockin-family sponge protein; inhibits type I Thoeris by trapping 3′cADPR. PF26760 MNMDKKEEKKEERPLDTTTNEQARERGVEIVGHQLWRTVVKAWDKKAGWMKSTKALEIPGLGVLVQVTTKEGSSLAEATCFVIGATLQDMGDGTYSITRP 6691 6993 + 3922 10214 100 6.9831 11122.72 0.5 0.75 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_2998,phrog_6518,phrog_6102,phrog_770,phrog_2729 APIS204 1 dpda restriction-modification (RM) Hutinet et al., 2019 https://www.nature.com/articles/s41467-019-13384-y YP_009196829.1 https://www.ncbi.nlm.nih.gov/protein/YP_009196829 APIS204.hmm Escherichia phage CAjan GCF_001501655.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:31784519 https://pubmed.ncbi.nlm.nih.gov/31784519/ In Enterobacteria phage 9g, dpdA is associated with G+ synthesis. This modification is proposed to play an anti-restriction role because 7-deazaguanine derivatives can block the activity of a wide variety of restriction enzymes MNVFMAAVYTNGYMPGQQRYEKLSEHEKNITRNLPHILESYHYVGRQKYVDQMRADGAKVFLDSGAFSAHSLGAHIDIVEYCEYIKRNKDILRVEDGAVMASVLDGIGDPLQTYRNQLEMEARGAKPLPCFHFGEDFRYLEYYMKNYEYITIGGMVGRSTDTLKTWLDRMWDKYICDGSGRAKIKLHAFGITSTTIMERYPWYSCDSSSWIQAAAFGSVVTPPWGPMRVSDKSPDRHHFGKHVSTLTEIEQDAVLKYLEQNGFTYERLSTIYESRAAFNLWAYGVIAANINATHDGTFRDRVMELF 28848 29768 - 25041 32082 383 306 6.7189 35185.83 1.5 0.9 restriction-modification (RM) CLAN028 APIS204 phrog_310,phrog_991,phrog_84,phrog_38,phrog_457 APIS281 1 fole restriction-modification (RM) Hutinet et al., 2019 https://www.nature.com/articles/s41467-019-13384-y YP_009032327.1 https://www.ncbi.nlm.nih.gov/protein/YP_009032327 APIS281.hmm Enterobacteria phage 9g GCF_000920815.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:31784519 https://pubmed.ncbi.nlm.nih.gov/31784519/ Enzyme to synthesize deazaguanines in G+ synthesis for anti-RM PF01227, PF14489, PF01227, PF14489 MNEFTKMTNRAEISHSIEKILKLIEGGDDLREGLVETPDRVAKAYATWFGGYSVDIASLFKTFEDGGENCDEMVIVRDIPVYSHCEHHMAPIIGHAVVGYVPNGKIVGLSKLSRVVDAFARRLQVQERLTNQIADAIVEHLDPKAVCVYIDAKHMCMESRGVKQVCGSSTITKAFRGRTALGDGVLPDVTGDTWRREFLEACKK 4030 4644 - 36 9105 65 204 6.5969 22640.99 0.5 0.84 restriction-modification (RM) CLAN035 APIS281 phrog_2335,phrog_2220,phrog_1887,phrog_262,phrog_5616 APIS294 1 qued restriction-modification (RM) Hutinet et al., 2019 https://www.nature.com/articles/s41467-019-13384-y YP_009032328.1 https://www.ncbi.nlm.nih.gov/protein/YP_009032328 APIS294.hmm Enterobacteria phage 9g GCF_000920815.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:31784519 https://pubmed.ncbi.nlm.nih.gov/31784519/ Enzyme to synthesize deazaguanines in G+ synthesis for anti-RM PF01242 MGYTVIRSHEICAGHRVVGHESKCRHLHGHNYKFHFKVAPKKTLSEGYVKSVSGFALEGSLDDVGRVIDFSVVKTTLCQWLEDNWDHKFLHWEQDSMINSLIVVASTKFARENNLVTDEDYDPFFNSLVALPFNPTAENLAAYMVDVIGPQLLDQYGVELVECTIEETSKCHVNYCK 4671 5204 - 36 9924 487 177 5.9582 20125.81 -3.0 0.86 restriction-modification (RM) CLAN049 APIS294 phrog_1204,phrog_196,phrog_102,phrog_2689,phrog_52 APIS363 1 quee restriction-modification (RM) Hutinet et al., 2019 https://www.nature.com/articles/s41467-019-13384-y YP_009032331.1 https://www.ncbi.nlm.nih.gov/protein/YP_009032331 APIS363.hmm Enterobacteria phage 9g GCF_000920815.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:31784519 https://pubmed.ncbi.nlm.nih.gov/31784519/ Enzyme to synthesize deazaguanines in G+ synthesis for anti-RM PF04055, PF13353, PF04055, PF13353 MFAQAIIAHHWAQAKFGNHMKIINTQEPEKLVRERGILDVHSIFHTIQGEGPFCGQPAVFVRLAGCNLQCPGCDTNYTSNRKKMNHGDIWQEIVRVTGEAKTDLVVITGGEPFRQPEVANFINYLIDMKGYRVQVETNGTMPIPRELNYNCVVVCSPKAAKVHPSVSARADAFKYVMKSGNVNEEDGLPLQALDHRATPFIARPPVHFKGKIYLQPMDEQDDDANKANAQAVVKYAMKHNYIVQLQIHKYLGVE 7448 8212 - 3007 13888 496 254 8.0103 28539.76 7.5 0.88 restriction-modification (RM) CLAN103 APIS363 phrog_24,phrog_2206,phrog_3573,phrog_84,phrog_6090 APIS276 1 jss1_004 Dnd,QatABCD,SIR2+HerA,DUF4297+HerA,CRISPR‒Cas Jiang et al., 2024 https://www.nature.com/articles/s41564-024-01851-2 UVK85907.1 https://www.ncbi.nlm.nih.gov/protein/UVK85907.1 APIS276.hmm Salmonella phage JSS1 GCA_030515965.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Salmonella; 590 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella PMID:39506096 https://pubmed.ncbi.nlm.nih.gov/39506096/ JSS1_004 employs N-terminal Ser/Thr/Tyr protein kinase activity to catalyse the multisite phosphorylation of host DndFGH. It also phosphorylates other bacterial immune systems to varying degrees, including CRISPR‒Cas, QatABCD, SIR2+HerA and DUF4297+HerA. PF27024, PF27024 MNYTDIQARLAIIKSLPISELDKRQPLLVALAADIVNGETSDGNDTDGSNGLEYQDWWHTLGALMRDAGFRMLGNGHFSAAYSHELLPGRVIKVGFKKEDSGAAYTAFCRMHQGRAGIPNVYHVARHAGCYTVVLDRLQSCDRYANDVHAKYASAAQEFIECTGGDADWYAQYVSNEFIETCKMIREFFHGIASFDMHSGNIMFDDNDVPYITDPVSFSHDREREDGFPLDPEALLAEVEAVAQERMIERCRNRKAKCDPNGTFQVNRKAAMKRRKRNRKLRAKVAERDRLHFMAIRRERGVIERNERRAEMLMGSAWHDFWLRNGNATVRKIDQVNGLKWQLGDRLAIQAGLPLNIDKVLDAHLMG 1947 3050 + 1021 8205 479 367 7.5056 41724.29 6.5 0.6 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_1330,phrog_405,phrog_964,phrog_38,phrog_9 APIS355 1 T5.014 bNACHT Kibby et al., 2023 https://www.sciencedirect.com/science/article/pii/S0092867423004117?via%3Dihub AAX11952.1 https://www.ncbi.nlm.nih.gov/protein/AAX11952.1 APIS355.hmm Escherichia phage T5 GCA_002921405.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:37160116 https://pubmed.ncbi.nlm.nih.gov/37160116/ The gene orf015 (T5.014) alter the activity of a wide variety of bNACHT genes and provide evidence for a complicated relationship between phage genes and bNACHT-based host defense systems, where orf008 activates and orf015 inhibits bNACHT proteins. MIRNVSLARSKGFKLVDVNTFEREDCKIEYVARNKNAFRVTEKKFDKRGNVIAETVKHFATFYAAFRGVL 8007 8219 + 5662 12848 56 70 10.6562 8145.44 7.5 0.59 bNACHT CLAN102 APIS355 phrog_2276,phrog_329,phrog_290,phrog_5879,phrog_4120 APIS292 1 dap1 Lon-mediated antiviral defense Le et al,. 2024 https://www.nature.com/articles/s41564-024-01719-5 YP_009224696.1 https://www.ncbi.nlm.nih.gov/protein/YP_009224696.1 APIS292.hmm Pseudomonas phage PaoP5 GCF_001551785.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa PMID:38886583 https://pubmed.ncbi.nlm.nih.gov/38886583/ Dap1 directly binds to phage HNH endonuclease, prohibiting host Lon-mediated HNH degradation and promoting phage genome packaging. MKTKEIDVSNFTAEQFDAFLEYCQYAQLKVGEGVVDKIREWMEHPISVDGPDDSPRCLYIRDHPEDRFISYGTGLRSKWQPDMYALYRPTFQTKCVMTLGEADKYVIVGGKPLSLDELQATLAKQGVIVTFGRV 764 1168 - 286 3260 31 134 4.9495 15303.48 -3.0 0.3 Lon-mediated antiviral defense CLAN096 APIS292 phrog_1762,phrog_3141,phrog_6444,phrog_2488,phrog_296 anhur 1 anhur Septu Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 anhur Vibrio phage 1.196.O._10N.286.54.E12 MG592565.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio lentus 136468 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio lentus Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Phage-encoded anti-Septu protein; inhibits the Septu/PtuAB ATPase–nuclease defense system and allows phage escape from Septu-mediated abortive infection. MAIVWLKLGCRCWCFYVGKRTPPPRSTRGVLMATGSY 37 10.4495 4206.09 6.0 0.29 Septu CLAN109 anhur phrog_10477,phrog_3575,phrog_1069,phrog_6435,phrog_815 APIS213 1 surt AbiU Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 AUR96245.1 https://www.ncbi.nlm.nih.gov/protein/AUR82831.1 APIS213.hmm Vibrio phage 1.217.O._10N.261.45.A1 MG592588.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Phage anti-AbiU protein; counteracts AbiU abortive-infection defense and restores phage propagation in AbiU-containing hosts. PF10076 MGHSVEQWTNSIMAQMPRGILWQRSASLDLYKYAAGYAPRLEAVEVSADSLLLEMRPENTQQLLDEWEEYLGLPECQVQNQTFESRRAAVVEKYHRKGGLQAWNIDKLGADLGFEIEVEEIFPHHCLRGCTYPLYEEKYRHLLRIHVRGITQAYATCLDDCLTPLVSQTAAILECTLNQFKLGGKYYEFIYEESV 29954 30541 + 25412 31485 190 195 4.8251 22513.58 -7.0 0.74 AbiU CLAN013 APIS213 phrog_6088,phrog_1775,phrog_1980,phrog_110,phrog_2231 APIS282 1 svarog Septu Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 AUR84964.1 https://www.ncbi.nlm.nih.gov/protein/AUR84964.1 APIS282.hmm Vibrio phage 1.066.O._10N.286.46.E8 MG592443.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio lentus 136468 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio lentus Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Anti-Septu phage protein; functionally suppresses Septu defense, likely by interfering with the PtuAB effector module or its activation. MEAIIKFALLIAWVAGFVIAKGFWPTLFCVIPFWSFYLVVEKVMTVLGWL 5 50 7.9858 5766.13 1.0 0.66 Septu CLAN095 APIS282 phrog_9306,phrog_3287,phrog_677,phrog_6435,phrog_380 hades 1 hades DRT type I Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 AUR85003.1 https://www.ncbi.nlm.nih.gov/protein/AUR83601.1 Vibrio phage 1.066.O._10N.286.46.E8 MG592443.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio lentus 136468 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio lentus Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Anti-DRT type I protein; phage-encoded inhibitor of DRT/retron-like defense, preventing type I DRT-mediated restriction or abortive infection. MTKFLSHLMLATYQSNTHKFMSDGEMGKIYEVTHAHVSYSRKRLIKEGHSFAFNTSVTKSNMPVQTYKYVGQTNLGLVCVDEKKDAMDGDTLLRLRYRMQSPSVPARQFPVEPGHVSLLGISSLV 125 9.8440 14113.27 8.0 0.29 DRT type I CLAN054 hades phrog_9959,phrog_5313,phrog_3677,phrog_1565,phrog_2998 kali 1 kali DRT type III Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 AUR90263.1 https://www.ncbi.nlm.nih.gov/protein/AUR90196.1 Vibrio phage 1.139.B._10N.261.48.C6 MG592511.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio breoganii 553239 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio breoganii Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Anti-DRT type III protein; viral counter-defense factor that suppresses type III DRT activity during phage infection. MGHKVAKCIFYDEASNIVFSAHGRPAKNMFIPVPQRGDWSDGMPKLYGAFSAGAIDRRREGITVHYRCAGFIKSTSVPGLVSRVPWLKL 26464 26733 - 18548 28273 89 10.4576 9866.49 8.5 0.2 DRT type III CLAN115 kali phrog_6102,phrog_345,phrog_5939,phrog_988,phrog_1056 enki 1 enki AbiH,Retron type II Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 enki Vibrio phage 1.080.O._10N.286.48.A4 MG592455.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio lentus 136468 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio lentus Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Broad anti-defense protein reported against AbiH and type II Retron systems; likely helps phage evade abortive-infection/retron-associated immune responses. LNVAPFIRPLFLLVKSFFYHSYIPIVVKFKCLKCDVRAKRRAFSLVRHTLDI 52 11.0031 6180.55 9.0 0.34 AbiH,Retron type II CLAN113 enki phrog_5929,phrog_4191,phrog_8689,phrog_2668,phrog_819 nergal 1 nergal Type I CBASS Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 nergal Vibrio phage 1.209.O._10N.222.52.B2 MG592579.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio breoganii 553239 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio breoganii Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Anti-CBASS type I protein; inhibits cyclic-oligonucleotide-based CBASS defense, probably by blocking signaling or effector activation. MSKHRGKAKRRATWVQTKRSPKNKHRWETEVNRARRKLAYAEYHKMKNRRGRNYV 55 12.3059 6851.94 18.5 0.43 Type I CBASS CLAN116 nergal phrog_8034,phrog_1273,phrog_4197,phrog_3011,phrog_3088 tlaloc 1 tlaloc AbiH Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 tlaloc Vibrio phage 1.056.O._10N.261.48.C11 MG592436.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio lentus 136468 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio lentus Lopatina et al., 2024 https://www.biorxiv.org/content/10.1101/2024.06.14.598830v1 Anti-AbiH protein; phage inhibitor of AbiH abortive-infection defense, allowing phage replication despite AbiH activity. VEVFLYLVHVHQLTYCLLMIYINCFQMYLYFLRRRGAIILSLLTGIDPRLQQFCTKSIF 59 8.7145 7119.63 4.0 0.46 AbiH CLAN118 tlaloc phrog_4406,phrog_3243,phrog_7313,phrog_2083,phrog_160 APIS244 1 orf72 BREX type I Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98980.1 https://www.ncbi.nlm.nih.gov/protein/WZK98980.1 APIS244.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF72 has anti-Brex type I activity, was predicted to be a Plasmid replication region DNA-binding N-term MSKVTHREQDKMRYTMKTNARRALKTRWGQVARDAHEQLIHQDEIGFYIVEKEVLQVLRDHKKAQQPEQKVPAPTAAPASSVSVLRPMVNPEIVKSCLEALEQEEEKDMKHAKAATQVSEIKIEEPKAAAPKQDVKTARCDFKNGARKPLKGKTAEVWAMGDKLLKELGRTPELKEMKAAMPSYNGTTVAIQFYAWRKYNGLDA 52006 52620 - 50784 53667 29 204 10.0390 23125.69 11.5 0.38 BREX type I CLAN050 APIS244 phrog_469,phrog_2885,phrog_124,phrog_2854,phrog_50 APIS307 1 orf65 hhe Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98970.1 https://www.ncbi.nlm.nih.gov/protein/WZK98970.1 APIS307.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF65 shows anti-hhe activity, predicted as a transcriptional regulator protein (SplA). MVMLNKVYSTMGADYKVVRHPRTGKFEAYIRPMGEGWMYYGTYDDAEQAYKEAESAAEASEIMARYDVDDYYLS 46513 46737 + 45059 51041 20 74 4.3855 8600.61 -4.5 0.34 hhe CLAN099 APIS307 phrog_1775,phrog_9228,phrog_778,phrog_9148,phrog_6435 APIS312 1 orf55 AVAST type III Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98958.1 https://www.ncbi.nlm.nih.gov/protein/WZK98958.1 APIS312.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 It has anti-AVAST type III activity, was predicted to be a ATP-dependent DNA ligase. PF14743, PF01068, PF14743, PF01068 MAFKPHLATDAVEEKIKFPCCILPKIDGVRGLNPDGRIVGRSLKLFKNRHTSAIFSGPQYMGYDGELAAGVETDPDLCRKTTSAVNTIEGEPFLKWHIFDLCAESVAELGYEARYNMMKDFITTQHAKGELLDLQVVPMYVVKSLQELLYWENIWLDMGYEGIIIRDPEKPYKHGRGTVREGGYLRIKRFIQEDAIVLDIIEGETNLNEATVNELGRTTRSSHQENKVPNGMIGTLVCKDVKTGNTINVSPGKLTQEDKIYYWNNPDKIKGRTISYKHFPHGVKDKPRFANFMHFRDESDQALD 40483 41397 - 35296 44481 260 304 6.7635 34586.51 1.5 0.79 AVAST type III CLAN022 APIS312 phrog_167,phrog_77,phrog_991,phrog_5342,phrog_964 APIS325 1 orf46 BREX type I Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98948.1 https://www.ncbi.nlm.nih.gov/protein/WZK98948.1 APIS325.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF46 exhibits anti-Brex type I activity, as a trimethylamine methyltransferase corrinoid protein. MNEETVILMQKIQRLQDELNDAICRAASKKIHSELEIHQRQVTSEAVVEQVVVNLNISLKGF 33199 33387 - 30463 35832 16 62 5.6244 7089.17 -1.0 0.48 BREX type I CLAN101 APIS325 phrog_6005,phrog_8841,phrog_2331,phrog_6705,phrog_3171 APIS345 1 orf58 Sir2-HerA,DUF4297-HerA Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98961.1 https://www.ncbi.nlm.nih.gov/protein/WZK98961.1 APIS345.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF58 has anti-SIR2+HerA and DUF4297+HerA activity as well as Reron Ec86 sensor activity, was predicted to be a “Mu-like prophage host-nuclease inhibitor protein Gam PF23791, PF23791 MDVLLYIHRNTTEDRKMFDADKAVQATEADVIEWYKTKAELQKLQARERALRDKIIKSYFPAPSEGTNKVEITGGVMKMTHKIDRKIDLPSLNGILGDLIKVGVNVDQLVENKPVLKVAAWRKLTAEQAAVFNQCVESKVGSASLEIVPNKA 42937 43395 - 38802 45667 143 152 9.3892 17006.72 4.0 0.47 Sir2-HerA,DUF4297-HerA CLAN032 APIS345 phrog_454,phrog_8147,phrog_1762,phrog_3918,phrog_5687 APIS354 1 orf83 AVAST type III Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98996.1 https://www.ncbi.nlm.nih.gov/protein/WZK98996.1 APIS354.hmm Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF83 exhibits anti-AVAST type III activity, was predicted to be a “nucleotide-binding protein DprA/Smf involved in DNA uptake”. PF10686, PF10686 MLLVVTGGRDFKHAEYIYAQLDKLHMQRRITTLRHGDADGVDRICAQWAERNGIKTEAFPAPWDNLSLPNTKIRYNAKGAYNAQAGAYRNQLMLNTDPKPDHAIVFPGGAGTMDMYRRIKASGIPYTLAE 56640 57032 + 54731 58842 46 130 9.5937 14593.65 6.0 0.88 AVAST type III CLAN012 APIS354 phrog_1171,phrog_2998,phrog_4217,phrog_2979,phrog_5302 Druad1 1 Druad1 Druantia type I Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 WZK98979.1 https://www.ncbi.nlm.nih.gov/protein/WZK98979.1 Escherichia phage Dru_SM1 GCA_038396275.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Ojima et al., 2024 https://www.biorxiv.org/content/10.1101/2024.04.14.589459v1 ORF71 exhibits anti-Druantia type I activity, was predicted to belong to a family of unknown function (DUF6614). MFTLEIKYFGSDWEVEDVFNNRYDAEQTGKFMLSQGLITNWRVL 51818 51952 - 49922 53545 44 4.1934 5292.97 -3.0 0.18 Druantia type I CLAN106 Druad1 phrog_4033,phrog_5183,phrog_10379,phrog_8014,phrog_3575 APIS207 1 adfB toxin-antitoxin (TA) Patel et al., 2024 https://journals.asm.org/doi/full/10.1128/mbio.00111-24 AXY82238.1 https://www.ncbi.nlm.nih.gov/protein/AXY82238.1 APIS207.hmm Vibrio phage ICP1 GCA_003575525.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Pseudomonadota Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:39287445 https://pubmed.ncbi.nlm.nih.gov/39287445/ AdfB functions as an antitoxin, abrogating DarT toxicity through direct interactions. MKIINFVNPNNTMDGYKLLVHCVFSGFDYDNTKFYMNREGFYLPEDQFNAFKSCCDIMMDLGIGDRKKEVSLVQGG 86194 86424 - 85077 89180 6 76 4.9295 8775.07 -1.5 0.74 toxin-antitoxin (TA) CLAN092 APIS207 phrog_9306,phrog_8841,phrog_148,phrog_4390,phrog_437 APIS269 1 oad1 Class 1 OLD nucleases Patel et al., 2025 https://www.biorxiv.org/content/10.1101/2025.01.06.631583v1 QVW06719.1 https://www.ncbi.nlm.nih.gov/protein/QVW06719.1 APIS269.hmm Vibrio phage ICP1 GCA_020494455.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio;s__Vibrio cholerae 666 Bacteria Pseudomonadota Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio cholerae PMID:39829814 https://pubmed.ncbi.nlm.nih.gov/39829814/ OLD anti-defense 1 (Oad1) is a direct inhibitor of Vibrio cholerae-encoded Class 1 OLD family nuclease. MCTHIITISGEKLDELGKLIDHKHNINFSTKFSIYEKVPSTGADISESNDSKDKIRQQAKDILDKIQSHQEEEESTPEWMEGFEDLIRSDYVLRKGDYLLLEDLSLEQKVFLQNSLKTTTEFTFLEEDYNYAYFNSKGSFVGLNYGDPFVVKKITFNDLFIPKQ 118079 118573 + 112338 121505 10 164 4.5343 19050.36 -9.0 0.22 Class 1 OLD nucleases CLAN094 APIS269 phrog_4201,phrog_1497,phrog_1538,phrog_3940,phrog_1098 APIS308 1 orf35 Tmn Yamashita et al., 2025 https://www.nature.com/articles/s42003-025-07730-8 WZL04578.1 https://www.ncbi.nlm.nih.gov/protein/WZL04578.1 APIS308.hmm Escherichia phage SM_S22 GCA_038425565.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:39987292 https://pubmed.ncbi.nlm.nih.gov/39987292/ Deletion of ORF35 from the ΦSMS22 genome resulted in reduced infectivity against E. coli expressing Tmn, suggesting that ORF35 functions as an anti-Tmn MKILQEEKDYWAAQCLEAREHAERVSALADTYLASYEGEEKARKELEERFDLLYELMGIMQRTGYTTTVALNRITADGLREHCKRAEEYVRPKGRQSSIARKDLVLMAWRNPTNGSFYPDERPLSAEESKRVARVINKGGKTPLYAIDTDHAEE 25924 26388 - 23579 29347 92 154 5.7878 17757.00 -1.5 0.39 Tmn CLAN100 APIS308 phrog_4406,phrog_4871,phrog_106,phrog_2263,phrog_3181 APIS240 1 Tad6 type I Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0105013_100020934 APIS240.hmm IMGVR_UViG_3300007510_000004 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB PF25188 MKMYVKAVLSVRRYKAGYEVREELVAGNQFDMDDIKVKTAYTPDGHYIGDSKTAYRLCKKRGIKPEPIDSEHNVCSIGFCEKEQKWYGWSHRAIYGFGIGSTCKKGDCHYVPTSFEEIQVDCYAKEEDDCVANCTVALEPVNPDEPERVQRAIPDSEEGRCVCAQENCVFEVGRGEWVAKTLDDAKQMAVDFAKSVA 27685 28278 - 24570 30063 17 197 5.0197 22231.11 -5.0 0.79 type I Thoeris CLAN016 APIS240 phrog_3904,phrog_1469,phrog_1293,phrog_4747,phrog_8841 APIS242 1 Tad7 type II Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0114343_100093911 APIS242.hmm d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ The conserved loop of Tad7 binds the catalytic E99 residue of type II ThsB via a hydrogen bond.   METKEIVELMLKSSDHNPYTGMLSKKDNLIAAIDLAKLCKDFADNGQTDEAMGIPSEQWDEVIDELNNL 4853 5062 + 3004 7948 37 69 3.9693 7741.72 -8.5 0.79 type II Thoeris CLAN033 APIS242 phrog_3545,phrog_8323,phrog_5137,phrog_8735,phrog_7507 APIS258 1 Tad4 type I Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0307375_1001427612 APIS258.hmm d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB. PF25186 MRLKDAEGWQKSREANQDPYGKAGLDYAERWAEMMEQWIPEDSTEKFITQQIENVAERCSHVADTEGITGFMYGCAVGLLSQVWEHGDALRRWHNLDCQIGTEGEEANESGKILNPAILDIK 4209 4577 + 2427 6720 97 122 4.3185 13858.40 -9.5 0.87 type I Thoeris CLAN015 APIS258 phrog_5311,phrog_61,phrog_3956,phrog_863,phrog_702 APIS270 1 Tad8 type II Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0080708_10004799 APIS270.hmm IMGVR_UViG_3300006145_000065 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ The conserved loops in Tad8 occupy the pocket within ThsA responsible for His-ADPR binding. PF25189 MKLFRNKETNKEIAERLIKLTDGKDYGIFAPPMKAQVAVDELCRYFLGEDWYSVNPISNEQINTEIVYEIECRFKKIKRG 2502 2744 + 1164 4142 29 80 7.7790 9446.93 1.0 0.76 type II Thoeris CLAN034 APIS270 phrog_2689,phrog_2729,phrog_3545,phrog_3940,phrog_2467 APIS351 1 Tad3 type I Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0172379_1000020175 APIS351.hmm IMGVR_UViG_3300014208_000032 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB. PF25185 MNSITHAEFEFSLLENVKYETEDEVPIVLEYKEEIINLIKKFSNSGQSGMSAPITASIITNCIKNLMAFKPIGPLVGNEEEWNYNSDDSFQNNRLSAVFKTGLNGKPYYLDAITFVGEEEYDTFHGHVEGISSRQYLKGFPFFPKTFYINVYKDFENKDENNLCSGDDGEYTYRIKYPEQLEEVFNYYDKFTKE 82 194 4.2938 22537.06 -13.5 0.75 type I Thoeris CLAN014 APIS351 phrog_4197,phrog_2885,phrog_107,phrog_85,phrog_1107 APIS369 1 acb3 type III CBASS Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0194137_1000084820 APIS369.hmm IMGVR_UViG_3300018411_000090 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ Acb3 encodes a conserved loop that is predicted to block the active site of CD-NTases and interact with the nucleotidyltransferase catalytic residues via hydrogen and ionic bonds.  PF25187 MKEVYKHEFNYWQQLIAMLTTMWRINKDSIDFKWGYFAPRFGLELRLNRGGYFDPYYAIAFCFIWGKFHIKLPFKTSLGEGCDLPKYGFTVSSNTLMLYWGGKFDNSIGQTNSRLKCWDLPFISYVFEHHKVLNKQGTWEDGTASYDNQNINRESYPYTYVLKSGEIQQRTATCFVEERQWHRKWLPWVKLVKPTISIEFSSEVGERVGSWKGGVTGCGYDLLPDETIEECLKRMELTRKFT 17352 18080 + 13595 19197 33 242 8.4366 28590.70 6.5 0.72 type III CBASS CLAN007 APIS369 phrog_2,phrog_1200,phrog_6468,phrog_424,phrog_5667 Tad5 1 Tad5 type I Thoeris Yirmiya et al., 2025 https://www.sciencedirect.com/science/article/pii/S0092867424014788?via%3Dihub Ga0224422_1102149568 IMGVR_UViG_3300021400_000048 d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis 1423 Bacteria Bacillota Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis PMID:39855193 https://pubmed.ncbi.nlm.nih.gov/39855193/ Tad5 can interact with E85 and other active site residues in ThsB but does not completely block the active site pocket. PF25190 MKRLLTLRNMIKSTTGVITVMAPGYKPRNIQAIVEYIIKYFNENCSDQEGYWKSNKSVMFYIDSDNIYDFVDKMLKGCKEFNELNLSQYEVDRGITVDDSSRPAWVIGGTSTGDHLKEYYDFIDIDACVRNISGELYWGFLDNDLYEGKVEIVNISEAAE 160 4.3600 18434.80 -8.5 0.78 type I Thoeris CLAN052 Tad5 phrog_1775,phrog_988,phrog_1314,phrog_460,phrog_1218 APIS279 1 T5Dmp Kongming Zeng et al., 2025 https://doi.org/10.1126/science.ads6055 AAS77048.1 https://www.ncbi.nlm.nih.gov/protein/AAS77048.1 APIS279.hmm Escherichia phage T5 GCF_000858785.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:39977546 https://pubmed.ncbi.nlm.nih.gov/39977546/ A deoxyribonucleoside 5′-monophosphatase functions as an anti-Kongming factor. PF26880 MNQVKTNITRNFPHISRVMIWDLDGTIINSFHRVAPCFDSEGNLDLNKYKNEACKHDLIMQDTLLPLVTYMRQCMNDANTLNIICTARLMSKSDYYYLRKQGLRGRGDSNIRVFSRDTLHKYFEADKVSEIYHSKDAVYKSYYFGLFKQLYPNADFTMIDDHKGVLSAAASYGFKTLDAQAVNDILSIGVTLIGETFIDESLEDDNDYQFLADRLQLCWEGMTEEERAEYSCSPQQYIEKLKVA 245 244 5.1497 28224.95 -5.0 0.78 Kongming CLAN021 APIS279,APIS286 phrog_310,phrog_10,phrog_305,phrog_684,phrog_5313 APIS279 1 bas26_0202 Kongming Zeng et al., 2025 https://doi.org/10.1126/science.ads6055 QXV79779.1 https://www.ncbi.nlm.nih.gov/protein/QXV79779.1 APIS279.hmm Escherichia phage GreteKellenberger GCA_020892405.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli 562 Bacteria Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli PMID:39977546 https://pubmed.ncbi.nlm.nih.gov/39977546/ The protein robustly suppressed cell death triggered by Kongming and their respective DNKs, indicating anti-Kongming function. PF26880, PF26880 MNQVNMNITRTFPHISRVMIWDLDGTIINSFHRVAPCFDDNGNLDLNKYSREACKHDLIMQDSLLPLVEYMRQCMNDANTLNIICTARLMSKSDYFYLRKQGLRGRGNSNIRVFSRDTLHKYFDANKVSEIYHSKDAVYKSYYFELFKQLYPNADFTMIDDHKGVLSAAASAGFKTLDAQAVNDILSIGVTLIGETFIDESLDDDNDYQFLAERLKMCWEGMTEEERAEYSTTPQQFIDKLKVA 110159 110893 + 106840 111980 245 244 5.1445 28203.95 -5.0 0.81 Kongming CLAN021 APIS279,APIS286 phrog_310,phrog_10,phrog_4372,phrog_332,phrog_326 APIS286 1 VPG01_164 Kongming Zeng et al., 2025 https://doi.org/10.1126/science.ads6055 QQO38522.1 https://www.ncbi.nlm.nih.gov/protein/QQO38522.1 APIS286.hmm Vibrio phage VPG01 GCA_024499575.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Vibrionales;f__Vibrionaceae;g__Vibrio; 662 Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio PMID:39977546 https://pubmed.ncbi.nlm.nih.gov/39977546/ The protein robustly suppressed cell death triggered by Kongming and their respective DNKs, indicating anti-Kongming function. PF26880, PF26880 MKKAYVFDLDGTTIDSFHRVEPCLDSQGNLNLDKYIKEACTPEKVADDTLLPLAGYMQQLIEQGELVIILTARFMLNHDYVYLRKNKLRAPIVCSRDQLPRVFGEGNAARISAMSDAPYKHEWFNHLFASMPQVTEWCFFDDHDGILEMANQLKGVTATDAKLMNELLQMQWADIYRQGEQDTAELIQDLIDECSTQDVICKPEWIDELLAQ 116438 117076 - 110568 119858 52 212 4.3356 24338.73 -13.5 0.81 Kongming CLAN021 APIS279,APIS286 phrog_2234,phrog_2885,phrog_205,phrog_971,phrog_877 dap2 1 dap2 Lon-mediated antiviral defence Zhao et al., 2025 https://www.biorxiv.org/content/10.1101/2025.03.13.642734v1 YP_009224697.1 https://www.ncbi.nlm.nih.gov/protein/YP_009224697.1 Pseudomonas phage PaoP5 GCF_001551785.1 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa 287 Bacteria Pseudomonadota Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa Zhao et al., 2025 https://www.biorxiv.org/content/10.1101/2025.03.13.642734v1 Dap2 directly binds to the Lon protease to prevent the degradation of the phage-encoded HNH endonuclease. MYDKAQVLLWVGDNLLDYTRKSDGSPMTFLWYEEELKEAIGYSKSTYDFRRVDFHFVTEKKVTAYTLHECDTGPTTELNGTYTLQALKEIIAEMEEENEN 1158 1460 - 286 3622 100 4.2249 11717.05 -9.0 0.3 Lon-mediated antiviral defence CLAN051 dap2 phrog_345,phrog_293,phrog_4747,phrog_206,phrog_8928 APIS201 0 IMGVR_UViG_3300008114_000032|3300008114|Ga0114347_100011773 APIS201.hmm PF10686 VDSEQIAMGFYLRQKAEEPKVTLVCGGRDFRDKDLLFRTLDSFNISCIVHGGADGADRLGGEWAIARKVPEVIVPAQWDNHGRAAGTLRNGWMLKFTKVEHVVAFPGGRGTLNMIQQTEKAGIQLTVISHG* 4 131 7.9145 14390.47 3.0 AVAST type III CLAN012 APIS354 phrog_687,phrog_29,phrog_2998,phrog_543,phrog_428 APIS202 0 IMGVR_UViG_3300020037_002393|3300020037|Ga0206644_10000862 APIS202.hmm PF26761 MTTFPKGSGTVMMTINNGVEAYKMRKFHLVRHVDVHGISGTGHVAEGVVFSDGTVAMRWLTATASSTFFNSVEDLLIIHGHQGATTVKWLEE 5 92 7.0727 10048.48 2.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_442,phrog_2979,phrog_148,phrog_2331,phrog_1211 APIS203 0 IMGVR_UViG_3300020045_010743|3300020045|Ga0206662_10111955 APIS203.hmm PF21825 MNSPHEQRVIDEKNQLDDKKDKLASFFSNDIFFGLSDVEQGLLHCQYQIMKAYSCILAERIKLFNKANQ 13 69 6.4453 8066.19 0.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_6102,phrog_8689,phrog_123,phrog_7140,phrog_3590 APIS205 0 IMGVR_UViG_3300046462_000343|3300046462|Ga0495651_0000268_29457_29744 APIS205.hmm PF21825 MIDEAGMMLLVEPIARSVDDYEREYMPLFPVRPDWQQRVIDEKAELDERIGKLCAFLRSDAYRALPDTDRFLLDRQIGHMRDYAHILFMRVARFG 4 95 4.8890 11306.06 -3.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_327,phrog_3285,phrog_453,phrog_2295,phrog_3245 APIS206 0 IMGVR_UViG_3300045836_000009|3300045836|Ga0466958_0000066_1255_1716 APIS206.hmm PF10686 VPNPPNHAYRLTDANPESIVSTPETMRRQHISWMRHSGTTVIVCGGRDYKDRDRVFAALDQMRAKHSGFLLIAHGGAPGADTLAGEWARERGIACEVFPADWEGRGPAAGPERNQRMIDAGANGCVAFPGGRGTADCVRRCEATGIPVWRPFG 4 153 8.0591 16580.66 4.5 AVAST type III CLAN012 APIS354 phrog_29,phrog_10818,phrog_8841,phrog_1649,phrog_514 APIS208 0 OP784576_00012 APIS208.hmm PF23791 MSDMALIPENTVSQEDLAEWYKLQEELKKVKAREMLLRTKIFKNCFPDPEEGTNNFNLPDGYVLKGKHTINRDVDPGAFQAMREQFTQAGIHPDTLVQWKPSLKIKEYRELTAEQLHLFDQCLIVKPGSPALEIVLPKKRASAAGPQNAGDNHS 48 154 6.4864 17458.95 0.0 Sir2-HerA,DUF4297-HerA CLAN032 APIS345 phrog_4913,phrog_6005,phrog_102,phrog_3364,phrog_737 APIS209 0 IMGVR_UViG_3300014493_000023|3300014493|Ga0182016_100017425 APIS209.hmm PF10076 MPTPPAFGDADYQQAMLRLLPRGRVWRRDPASTLSAVMLAVAPTYTRSTAAAAQVLVDASPATTVNLLDEWESSLGLPDPCTAPNPSIEQRQAAVRAKWGARGALTPAYFIAMAAALGFTITITEFTPFAVDMACDLALYEPEWAFIWQVTAPGGSTFYFSVDESSVDDPLETYDAGELVCRITQDAPAGTLVFFVFPGPVFVLDIPGLNLLDFGVLA* 21 218 3.9249 23398.69 -13.0 AbiU CLAN013 APIS213 phrog_2392,phrog_376,phrog_325,phrog_107,phrog_794 APIS210 0 IMGVR_UViG_3300025141_000036|3300025141|Ga0209756_100004674 APIS210.hmm PF26761 MKKQKNLKKTPVAMMIMNGSRRTNSMKIFYLNRTEDESGISGTGRVAQGFVFDNGKVAVTWLSEHPSVTVYDSIGEVHAIHGHGGKTEVVMEPDYKKAFGELKSFVDNFNMPEVVSSKLTPDGAASKLMSKA 51 132 9.9533 14498.65 6.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_381,phrog_428,phrog_1068,phrog_695,phrog_2083 APIS212 0 IMGVR_UViG_3300001748_001095|3300001748|JGI11772J19994_10021762 APIS212.hmm PF26880,PF25109 MIKQVTIFDLDGTVIDSSHRQVTDSKGNLNLAKWFENNTPEKIFQDKILPLAQEIRRRHKKGDYIIINTARNLSYADYEFMMENGILADKVIGRPQGNMENDAELKRKQLNSFLSLKQFKKANKVMFDDNNEVRSLLRQIGISVIHPNKLNERLK* 3 155 10.2088 18031.74 7.5 Kongming CLAN021 APIS279,APIS286 phrog_4747,phrog_687,phrog_3004,phrog_5667,phrog_1698 APIS214 0 IMGVR_UViG_3300010357_000570|3300010357|Ga0116249_1000073248 APIS214.hmm PF25185 MSDTRYAERELDILAEQIPNSVVLPFRDQILAICEAFLESGQSGMSAPFVATALSNTIKNYYSLNLYVILRVMKVNGMMCQDTRCSSLFKNENGICTYGQAIVWRGEQEYDTYTGGVYSGVGEFELISSSQCVKFPFKPKTFYVDVIRIPISKELADLRDIHYTEDGSGECYYSVVKNPKQLEEVFSYYTKRVL* 12 194 4.7374 22073.17 -4.5 type I Thoeris CLAN014 APIS351 phrog_1506,phrog_6616,phrog_67,phrog_4372,phrog_2871 APIS215 0 IMGVR_UViG_3300014204_000700|3300014204|Ga0172381_100017795 APIS215.hmm MKVYLAGAPGGGSTGLCRREKELNRLWKLRLWSYYHLILVNGIMPNENKKRVELFLDSGAYSAWAQGKEIDIQKYIEFIKENINVIDIYANLDVIGNAEATWKNQLIMEKAGLNPLPVFHYGEDIKWLKKLLDKQYSYISLGGMVPISTGDLFVWLGELFSNYLTDKHGMPIVKVHGFGLTSLRLMLKYPWYSVDSTSWVVTGRMGSIYIPRYRNGQWIYGEDSWKVAVSNKSPDKTEAGKHINTFSTEEKKILLRYIHEKGYSLGASKYKTVSSGYELEENEKFVDKKPTDKKAKREVEIIIEPGVSNMYQLRDEMNIIYFLDLEKSMPKWPWPFKKQNVQKGLF* 11 346 9.5875 40055.33 12.0 restriction-modification (RM) CLAN028 APIS204 phrog_31,phrog_1330,phrog_616,phrog_1156,phrog_708 APIS216 0 IMGVR_UViG_3300018063_000062|3300018063|Ga0184637_100017413 APIS216.hmm PF26761 MRLFKMVRDVDVTGISGTGVVATGVEFDDGFVVIRWQGERPSTVIWASLRDAEAIHGHEGKTRFVNLAFVHVEGPEQKPSAS 3 82 6.2139 8996.20 -0.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_5218,phrog_7158,phrog_81,phrog_1041,phrog_87 APIS217 0 KY940711_00071 APIS217.hmm PF10686,PF14216 MPAPKVYNKHHKNAPADARYIGRGSPYGNRFIIGEHGDRDEVCDLFEQEQLPNMDVRELANCDLVCFCAPHRCHGDSILLKANHRVLVFGGRKFDNRKALYRSLDVAHMLRKITCIIEGEASGADRMAREWAEEHGVPVDRYPAKWDDIDRPGARVRKNKFGKLYDADAGPLRNTIMLREGRPDKAIGFPGGSGTANMAKQCIEYGLTPIFPKVW 19 215 8.5861 24246.73 9.0 AVAST type III CLAN012 APIS354 phrog_6128,phrog_1272,phrog_2220,phrog_1469,phrog_381 APIS218 0 IMGVR_UViG_3300008255_002756|3300008255|Ga0100403_1000020114 APIS218.hmm PF26761 MSIVTKETKMRRFHFIREKDASGVSGVGKVAEGVIFSNGKVALEWFGSRSSTNLYNCLEDVEYIHGHAGMTKIVFEDPEEPAEDLTKDEMNNAN* 10 94 4.8838 10511.79 -3.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_864,phrog_6435,phrog_3918,phrog_6102,phrog_1211 APIS219 0 IMGVR_UViG_3300014204_000077|3300014204|Ga0172381_1000078054 APIS219.hmm PF25186 MKEYEPIPGTTIGNAAAQQRRDTVLKEALAGAPEKMTLLDADGWEKAVAANTDDYGSGVIRYAERWARLMEGRMIRGDTLEACAEEASHLADDEGITGFMYGCAVNILSKTWIHGDQLRRWHNLKVQIRDEGEKANRSGAVLNPAIITLGVSD* 3 153 4.8590 16787.91 -4.5 type I Thoeris CLAN015 APIS258 phrog_148,phrog_428,phrog_3964,phrog_3287,phrog_45 APIS220 0 IMGVR_UViG_3300020037_002740|3300020037|Ga0206644_100027631 APIS220.hmm PF26760 MFKEYIKKQTTSIAPIKEWLAIEGNTMDKVAVGKEHSTLPKEVFDAGYIAQDKTNPKDIYYIPRDLFINNYTEAGTTGKTLHNTDASGASVNVKDIVFWGDGDTFQLISKASSKKEGWMKSTKAMQIPKLGCVIQITTQQGDNVSETCCFVPGAIVQVNKDENGKVLSRVVTKGGY 10 176 8.2356 19376.10 3.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1200,phrog_4492,phrog_196,phrog_2083,phrog_1698 APIS221 0 IMGVR_UViG_3300044852_000001|3300044852|Ga0436595_000033_6213_6677 APIS221.hmm PF21825 MTQGGIMTQDYIGTKQIVAWEQDSQKKVKVCGIDCKKGDAACNSYCTGGADRAPAHAPEPGYAVKYPDGYVSWSPKAVFEAAYLPMGHIGHLPPHQQRVIGEKVQLDDRVSKLEAFAQTDFFKGLPEPDRDLLVTQYGLMAAYSEVLGKRIALF 4 154 6.8437 16909.32 1.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2662,phrog_67,phrog_5616,phrog_2373,phrog_1252 APIS222 0 MT028491_00053 APIS222.hmm PF10686 MGITVLICGGRDYADAAKVYDILDYIHKKVGIDVLISGAARGADMIGINWAEERGVRCLKFPANWKKYNKAAGFIRNKQMAECEPDICVAFSGGSGTNNMIKLCEQYGIKYKRYDYGK 34 118 8.8581 13125.23 5.5 AVAST type III CLAN012 APIS354 phrog_6079,phrog_1012,phrog_2402,phrog_1075,phrog_770 APIS223 0 IMGVR_UViG_3300043538_000005|3300043538|Ga0455948_0011080_2198_2443 APIS223.hmm PF26761 MRRFRCYRPHPPEGYRESGAANPPDEVQFEGVVFSDGTVAIRWLTEFRSHSIWASWADVEKIHGHPEYGTVIEWLDPEPVN 71 81 5.0735 9410.45 -3.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_595,phrog_778,phrog_8928,phrog_2373,phrog_4120 APIS225 0 IMGVR_UViG_3300028563_000018|3300028563|Ga0265319_100008553 APIS225.hmm PF26761 LRRFQLERDEDETGVSGEGIVAWGIEFPDGVCVTRWCVTDIRQTCVWASMTDIEYVHGHGGKTRIVYLDVA 36 71 4.5006 8044.06 -4.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_3869,phrog_4219,phrog_2331,phrog_4747,phrog_5127 APIS226 0 IMGVR_UViG_3300033146_000690|3300033146|Ga0366841_10263344 APIS226.hmm PF26760 MKVKFEQYKKTALQDMTPWKKGMTGIDIPTGVTPKEGDMVAKTGDDTWLITKEFFDENYQIAEPIRTKSLHNTTSNSARKNVKDIVFWGDGDTFKFISKASSESEGWMKSTKAMMAGAGVVVQVTTQQRNPDGSYSVAEALTYVPNVQIHEVLKEGKVVSRYLAR 4 165 9.5370 18462.04 4.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_29,phrog_3333,phrog_2871,phrog_973,phrog_3222 APIS227 0 IMGVR_UViG_3300035661_000566|3300035661|Ga0373633_0010963_6732_6929 APIS227.hmm PF21825 MKSYQERVVIERNELNTKIEALEVFIDSDNFKTLVSNKEQELLRYQYRSMVEYWAILNKRIAIYE 7 65 5.0824 7963.12 -1.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4211,phrog_3287,phrog_1716,phrog_746,phrog_1366 APIS228 0 IMGVR_UViG_3300042922_000692|3300042922|Ga0456363_0001473_30777_31238 APIS228.hmm PF26760 MKKMNKDLSVTSVEDAKTKVSDIIVSGDGDTFALLCKASSKEQGWMKSTKVCNVEGGCLVQVSTQQRNPDGSYAVAEALSFVPGIQLNKDAEPRRLEPIMQLNGLDSASYTSAEDSIYQRRMFLDSLIQAQNSGVNVRDMITSELEEMKSLIK 14 153 4.7914 16801.10 -3.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_196,phrog_2335,phrog_29,phrog_186,phrog_296 APIS229 0 IMGVR_UViG_2518645610_000003|2518645610|2519025895 APIS229.hmm PF26761 VTNPRRFHLQREHDVTGASGTGRVADGVLWPDGTATLRWLGPRASTVHWDQLADAVAIHGHGGHTHIVWDDPASAERETTEFAELLRQFVALTDTLHHTLGGPHDEVGPGLTCDGCRLADEATRALRDAGERTTLRSHLRSPREAGHDG 4 149 6.3561 16211.80 -1.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_2034,phrog_1775,phrog_678,phrog_9998,phrog_1885 APIS230 0 IMGVR_UViG_2767802354_000003|2767802354|2769867598 APIS230.hmm PF21825 MSPTGTNLLYFGQAKAMLEHCINGSTARASGPSPHQQRMLDEKQETDIRITKLDEFMLRNALFRQLDPEEQSRMRRQLDVMCELSVILGKRIARF 5 95 8.5442 10967.67 3.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3883,phrog_5302,phrog_1062,phrog_438,phrog_2263 APIS231 0 IMGVR_UViG_3300000405_000017|3300000405|LV_Brine_h2_0102DRAFT_10000645 APIS231.hmm PF25187 MIKFSFTDKDTDEYKSWFSFYPSRNKGTGFDLTYQSNGYFDPRPKVSSSITTLLALLLPFISFWLIPISLVFCFYSWGNLYVRLPFDTGRGNTAENKTYGLMFYHPDSGFPSEIWVRGWKSFSFPWAFKFLKCEALMNEGWVKGNKGDYFGDADKWGDKIIYDTYDYKYVLESGEVQERLAKVHQIKRYWTSWFGLNVLVKHVIEIEFNFEIGERSGSWKGGCIGCSWVIEEGETSLEALKRMENIRKF* 7 249 7.1858 29171.23 1.5 type III CBASS CLAN007 APIS369 phrog_991,phrog_202,phrog_2885,phrog_719,phrog_992 APIS232 0 IMGVR_UViG_3300033070_000668|3300033070|Ga0364486_104628 APIS232.hmm PF25186 MELKTFAGDSFNAVTLKAQEILNNSDNVLVQFDFNSVKCVVTAETNTEWLYRDYCNSLSYDIPKFATGTESEYSSDIQEQLNKAIIERENRQKAEQKKYQAAEKRKIAAFNKKVGGIKIELYNPLAWKMWVENNKDGYGKGIMTFAENWAKLMQVELAAGNELKNIASSTSNQADLEGITGFMYGAAVQVLSDSWKHGEKLRRWHNKEYNQEGDGVVNPAVLSVG 21 225 5.4392 25383.53 -2.0 type I Thoeris CLAN015 APIS258 phrog_971,phrog_3004,phrog_133,phrog_67,phrog_2263 APIS233 0 IMGVR_UViG_3300023211_000115|3300023211|Ga0255842_124164215 APIS233.hmm PF10076 VIVSLQEDYRWLFASLLPEGPIWPREADSDLGRLLGGLAAEFARVHGRALQLIEEIDPRTTYELLIRWESVAGLPDPCAGDVQSLAGRRKRLLSTITARGGARPAYFTALAADLGFYIEIDECLPASVDRDCNWQLWADTAAYCWRVRAPLTSVEYADCTSGCDEPLRLWGNAPLECAIERRKPAHTFVYFGYIDINDGGPITDPPEAVLRGGPIIEPYDRVLDFGAIA 7 229 4.3867 25367.80 -11.0 AbiU CLAN013 APIS213 phrog_10,phrog_1101,phrog_684,phrog_3883,phrog_1068 APIS234 0 IMGVR_UViG_3300033153_000136|3300033153|Ga0366824_10915457 APIS234.hmm PF26760 MSENIVREEGTSSANKPVKTQIINDVLVIDDPKAVLKPEPKSKVKSKVKAKPESLEPQPKTMGNTDQNGCRKNVKDVVMFGDDLFKLLSKASSESEGWMKSTKAMDTGKGCVVQVTTQQRNLDGSYAVAEALTFVPGVKILDQIYKGQLTGRKLVGVR 3 158 10.0402 17237.91 7.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_4197,phrog_617,phrog_4454,phrog_177,phrog_4219 APIS235 0 IMGVR_UViG_3300037245_001837|3300037245|Ga0392328_0000081_63494_64360 APIS235.hmm PF25187 MDNTAKQTPKNELHFEKKKEKVTFKFQTKFTERETEDHNKWLSFYPKKCYFRFQLDSWGYFDPRPQISTNITSVLVILTLLVSLFTLTITLYHLVLIPFLFFGWGELFLNLPFNSGKTDECENPSYGFYMYHIDPVPKKVNFPTCFIWQWKNYKSFNMPWARTWIRTSILLKDNTWAHETKGNYKSFYKDKWKKQQYMIEYDFTDKYDNTIIPTKVYVEEREWRQHWLKWTKLFALVRKTIDVDFSKEVGGKKGSWKGGTVGCGYNMKKGETALECIKRMEKERTFRS 16 288 9.8602 34744.09 18.0 type III CBASS CLAN007 APIS369 phrog_1535,phrog_38,phrog_405,phrog_8927,phrog_1293 APIS236 0 LC727895_00052 APIS236.hmm PF10076 MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERVAWTGKQLLAERFPDSSRLLLEDWERYLGLPECDMAGATITERQRYAGNKYRMKPSLNREFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALECILNRYKPAWQTFIYVYANSHEEENI 111 186 5.0574 21377.31 -3.5 AbiU CLAN013 APIS213 phrog_2662,phrog_1272,phrog_376,phrog_104,phrog_1107 APIS237 0 IMGVR_UViG_3300006052_000206|3300006052|Ga0075029_10000415813 APIS237.hmm PF01242 MIVATRRHEICCGHRIHLHESKCAHLHGHNYGFTFHCTAPGLDKAGRVVDFSVIKATLCQWLEEQWDHRTLICNEDPWAMTLQLLDRDGIVLVPFIPTAENLARWMVDVVGPDLLQGVTLVRCDVDETSKCSATYEREDPEKLKHHLVSLVQAGVISINEAHQQLEKYGL* 6 170 6.3514 19301.14 -1.0 restriction-modification (RM) CLAN049 APIS294 phrog_4211,phrog_4197,phrog_3288,phrog_906,phrog_684 APIS238 0 IMGVR_UViG_3300014205_004963|3300014205|Ga0172380_100250779 APIS238.hmm PF25188 VYSVEGDTDTVMSSVYSKEGKYITSVSEFVNLPNMTNFESAKPYNNNCNIGFSEDTNEWFGWSHRAISGFGVGSTCKKGNCHYNPNSFDEYRYGSTWGACSNLVESYSVQEGINKLYCYETSGMECTEDNCSQYVKGRGEWTALSLEDAKEMAKDFANNV* 3 160 4.2956 17917.53 -9.0 type I Thoeris CLAN016 APIS240 phrog_5311,phrog_67,phrog_2885,phrog_3004,phrog_2713 APIS239 0 IMGVR_UViG_3300020577_002214|3300020577|Ga0212215_1000037531 APIS239.hmm MRIYMAGLYTSSGKAVIGETTINQVITQNLVYPWMLESFHYIGEKPIVPKLLRERGESIFMDSGAFSMFTQGINIPLEVYADFIKANSDVIHVSSNLDKIGQGQEQGTWENQKALEALGVDVKPVHHVRDSDEWLLRYLAEGYDYIFLGGMVPESIPTLRRWLDHVWGKYLTDKDGFPKVKVHGFGLTTPELMLRYPWFSVDSTAWVLASRFGLITLDLPKDTGGFRDLKLAISSDSPANYKINGHYDSLDPLTRRAVDKRIDELGFDPELLRTHYGWRDTFNVRYFERMMSRATDRFRHAQATLSF 4 307 6.5936 35229.17 0.5 restriction-modification (RM) CLAN028 APIS204 phrog_1330,phrog_4527,phrog_24,phrog_202,phrog_2234 APIS241 0 IMGVR_UViG_3300017829_000032|3300017829|Ga0189853_100003716 APIS241.hmm PF25188 MHDVKREISRDHFDGFVLVKQLIDGSSYGSEDFEVTVALTPTGDYIGNEETARYIVGNRGIAPEKVEPLRTICSIGFCARERRWYGWSHRAICGFGIGSEVRRGDIAYVPTCWDDFKDDCIRFWTDPHHLDVKASNVAQDSVQGVYVSWTYSDTFGNEAMRGTIGGTFIAPPKQWGRGEWMAKSLDDAKQMAINYADDVA 4 200 4.9223 22502.13 -5.5 type I Thoeris CLAN016 APIS240 phrog_4211,phrog_2335,phrog_6117,phrog_262,phrog_3830 APIS243 0 IMGVR_UViG_3300037312_000536|3300037312|Ga0395899_0000236_64925_65458 APIS243.hmm PF25185 MKHLQELKQRIRLAYQIIRGRDGNLVRHTVNEFTVLGNFKETGPNNWIAHNVLDLVRVFATQGHSGSSAPFAIELFRKAANFDPLGPITGGASEWVEVCNGMWQNRRCSHVFKDSVDGPAYDSQAVIFEEPNGGRFIGRYSRQFITFPYTPRSVVAHVPSDATELDKKLAAETAWRG 3 177 9.0657 19887.47 6.0 type I Thoeris CLAN014 APIS351 phrog_4211,phrog_2229,phrog_2488,phrog_496,phrog_1314 APIS246 0 IMGVR_UViG_3300035676_000130|3300035676|Ga0316623_00001351_5430_5846 APIS246.hmm PF26760 VVYTTENSNQLNQYKMQEKSLHNTTANGATKNVKDIQFWGDGDTFRLISKASSESEGWMKSTKAMPVGNSVVIQVTTQQRNPDGSYSVAEALTTVDNVIIREYKVGDTVEARTIIPRTWEHEGVHVTAVRNVALETAE 4 138 5.6674 15331.04 -1.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1775,phrog_6102,phrog_2979,phrog_7158,phrog_5555 APIS247 0 IMGVR_UViG_3300025909_000037|3300025909|Ga0207705_100067743 APIS247.hmm PF21825 MSTTHITPTPGRIVWYRGADGEIRPAIVMKGNGPFNADLYVFPLSGADTDWGNKATVTHADPEQEPGCLQSWHWMPYQKQQAEKHANEMASTAGPKTGNADASEAARAEEPKFIGFTGASCGGIQLQVSTLQPHQQRVVDEKAELDERLAKLRTFFDTAIFGGLDAAEQSRLRQQAHAMACYSAILGERIAAFASAAESAAA 7 202 5.5814 21776.31 -3.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4197,phrog_202,phrog_1204,phrog_1272,phrog_469 APIS248 0 IMGVR_UViG_3300027819_000011|3300027819|Ga0209514_1000065225 APIS248.hmm PF26760 MTKKSTEAFEVRDLRGHGNVSEFKPKTLHNSDVSGAKKNVKDIVVVGNGDMFQLLCKASSQAEGWMKSTKACQTPHGCIVQVTTQQRNIDGTYSLAEALTFVPGVKIAADVSSGRKLV 13 118 9.7043 12724.56 6.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1802,phrog_4211,phrog_1698,phrog_2013,phrog_3964 APIS249 0 IMGVR_UViG_3300033148_003435|3300033148|Ga0366832_100118252 APIS249.hmm PF25186 MPISNQEKWQKYQDSNTDDYGEACVKVARRVMEILDEGDAFDPHDIICRADEETGVGGITGFMAGAMASMVSLCHSRGDEFRRKWNLDTQIQTEGEKANESGGVLNPALMSMREKK 8 116 4.5256 12946.48 -6.0 type I Thoeris CLAN015 APIS258 phrog_4406,phrog_1794,phrog_2987,phrog_4352,phrog_724 APIS250 0 IMGVR_UViG_3300027780_000012|3300027780|Ga0209502_1000022957 APIS250.hmm PF26880 MQKFKIWDLDGTVIDSSHRYSTLENGDIDLPRWIKNNTRENISLDKLLPLADLMRDNYRSGDTVIICTARVLGVWDRVFLADLGLQADYVLSRALGDTRKDAEMKRAKLLALFSDLNIPLARWTRNATFYDDNLGVLEMAKRLNIRAKNATILNNQMKAKA 3 161 9.7248 18428.24 4.5 Kongming CLAN021 APIS279,APIS286 phrog_2034,phrog_6005,phrog_116,phrog_291,phrog_4217 APIS251 0 LN610572_00006 APIS251.hmm MKERLVKFYTIVKTPEENIAVQQHLKAYGIEFGSGNGFMYDKAQVLLWVGDNLLDYARKSDGSPMTFLWYEEELKEAIGYSKSTYDFRRVDFHFVTEKKVTAYTLHECDTGPTTELNGTYTLQALKEIIADMEEENEN 24 138 4.5229 15998.99 -7.5 Lon-mediated antiviral defence CLAN051 dap2 phrog_4219,phrog_581,phrog_617,phrog_1218,phrog_5156 APIS252 0 IMGVR_UViG_3300047323_000157|3300047323|Ga0495683_0000328_14848_15588 APIS252.hmm PF11195,PF21825 MKTFIGTKLVKMLAMTRAEYNAFRGWELPANENGADDGYLVEYLDGGKPNTPQYAGYVSWSPKAQADAAYRPVTGMSFGLAIEAAKKGARIQRAGWNGKGQFVYLVPAASYPVQTGAAKAHFGIGSMVPYNAYLALKTVDETVSTWAPSVSDCLADDWLIVEDQAAAPVIGEDGPLLAEPKTLAPHQQRIVDEKAQNDERLSKLVDFIKTNPVFDKLPDAERLRLTRQHRIMDELSNVLGERIAAF 5 246 5.7799 26977.68 -1.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4197,phrog_346,phrog_992,phrog_1293,phrog_1216 APIS253 0 IMGVR_UViG_3300008110_000073|3300008110|Ga0114343_1000119107 APIS253.hmm PF25185 MNNRTFAEIELDIISKINPDFDILSIKNEILNLCKKFRDSGQSGGSAPYTAGSIIHEIKNKISSNIVLEIAKDPDSLTYEFTEEINSIVEVIKTKSINDFDHLIESINNLLLFHPIAPITGMDDEWGDVREFGDGNSWYQNKRCSALFKDGKDGKPYYIDAIIKRDQNNVCWSGFAWLSEEDYKNGDRSKMVGKKGYIKSFPFTPKTFYVDVRDVEVAKDDWESFIVDPKQLEEIWNYYDIK* 8 242 4.5603 27880.38 -10.5 type I Thoeris CLAN014 APIS351 phrog_5342,phrog_10,phrog_2335,phrog_1272,phrog_551 APIS254 0 IMGVR_UViG_3300028588_000054|3300028588|Ga0265780_1000121522 APIS254.hmm PF13876,PF21825 MNDQAIEQEIQAKGKTAPRVTPADIDANIASAHYFTAKDGVIGDLFTSGLIDEPNGHNLPPALPLLTFCVLTLRNGFTVTGESACASPENFDAGIGRKIARDNAVQKIWPLMGYALKQQMHQAANLPPALGAAHVGYSTLQPHQQRVVDEKAALDDKLAKLQQFVDGAIFGTLDEAEQSRLGIQLDAMAAYSEVLEHRIAAFEPANHPV 15 209 5.0153 22478.43 -5.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1535,phrog_2392,phrog_1506,phrog_262,phrog_5311 APIS255 0 IMGVR_UViG_2857096214_000002|2857096214|2857100266 APIS255.hmm PF26761 MTTQHTQYLVRSDTDLVRFHLDRHEDITGVSGEGTVAGGVIFPDGTVAMRWNTGTKSTAIYDSIDDVVAIHGHNGATVVTLIDSREADAE 4 90 4.5358 9713.66 -5.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_1775,phrog_3956,phrog_8917,phrog_453,phrog_1062 APIS256 0 IMGVR_UViG_3300000053_000065|3300000053|Draft_0800713 APIS256.hmm PF21825 MPTTTHKYFAFAHLPPKLQAVSKPIGELAAALEVLLPDGPEKSAGMRKLLEAKDCFVRCALDMPEPSASTVPPHQQRVIDEKAARDGEVSRLAAFIDSNPVFPQLPADEQARLRRQLDVMRELSVILGERIAAF* 14 134 6.7145 14757.11 0.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_454,phrog_687,phrog_4219,phrog_2263,phrog_773 APIS257 0 IMGVR_UViG_3300006641_000068|3300006641|Ga0075471_1000011729 APIS257.hmm PF25187 MNKFLKLIGYQHNYGGREPSYKFKWGEIALWSKGISFRWSGPHYSYEPKLIVALYFITFYINTPSFGVEAAQMCSEDERNYGFYLFPNLNNWETTVFEFHNKSKHINMPWTYKWKRTELLDWDMNTVLKEEAGERDFDKWYRESQAWVAINAKTYDYLYVLENGVIQYRKATCHIERRTWTVRWAPWINKVSTTLEVKFDDEVGERSGSWKGGTIGCSYEMLPNETPEQTLRRMEREREF* 4 240 7.3449 28860.61 2.5 type III CBASS CLAN007 APIS369 phrog_23,phrog_156,phrog_239,phrog_612,phrog_1775 APIS259 0 IMGVR_UViG_3300033120_000438|3300033120|Ga0364481_1009813 APIS259.hmm PF25187 MIKNNWLEFYPKFSNVTVRVSPASYFDSRWFINICLGWGHFYIHLPFRSKIDDCEYPEYGFYWYGEKGCNSIWLCWGYKKKCIYMPWALDWVRTSKLLNDNNWAHDTKKSRKDFWQDEWKDKIAYEKHPYTYTLKSGEVQHCMATINVEEREWRPRWFKWTTLFRKVRKSIGIEFNHEVGERAGSWKGGVLGCGYDMLANETPLQTLRRMEKERVFR 24 217 9.2378 26411.24 12.0 type III CBASS CLAN007 APIS369 phrog_454,phrog_1887,phrog_1107,phrog_2034,phrog_196 APIS260 0 IMGVR_UViG_3300033148_004627|3300033148|Ga0366832_100196437 APIS260.hmm PF26761 MTTKKTAQAKVFHLIRDEDETGISGTGIVADGCVFPCGTCVLRWRQDTNVKAQSTGVYESVEHVELIHGHNGKTRMVFPSSPSHDNS 10 87 6.9563 9515.67 1.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_6162,phrog_8841,phrog_1068,phrog_958,phrog_2631 APIS261 0 IMGVR_UViG_3300037455_000234|3300037455|Ga0394157_0000359_47972_48463 APIS261.hmm PF25190 MNNYMVIVSLGDFIKRTIGEIYYLAPNHNPRGINEIREAIISRFMDGCKQIGNDFFYHEIALKDLYKFISDILMPIEKFRELNLSQLEFDNGIKVDDEERDKFAFTDRYSSIPEYDIFIDLDACIQNIFSSFELEQFRIHLNGEIEIAELARFRNAYRQEKED 3 163 4.4102 19371.94 -10.5 type I Thoeris CLAN052 Tad5 phrog_454,phrog_3883,phrog_511,phrog_2979,phrog_6651 APIS262 0 IMGVR_UViG_3300044691_000180|3300044691|Ga0467056_0004566_3192_4124 APIS262.hmm PF01068 MIQVMRPANYDPKKIKFPVWAQPKIDGVRAFNPEGTLLARTLKQHKNRHVTEYYSQKQFIGLDGELAAQGETHPDLCRITSSALSRIEGQPYTQWHLFDWLTPNTIHLPYRLRYELLQQRVKIIDDNRLRVVPYTPCDNLEELMTTHEWNMQSGYEGTCFYGPDVIHKEGKSSPNHNGVLRIKDFVDTEVIVEDIIEGRHNMNDALVNERGLQYRTSHQDNQVPNGMIGSLTCRALATVTDLYDKKKILIEKDQIFTVAPGNMTDAEAKLFFEQPQLIVGKISKIKFFPKGIKDAPRFPQWQCLRSKEDL 22 310 7.7727 35823.98 6.0 AVAST type III CLAN022 APIS312 phrog_24,phrog_16,phrog_239,phrog_3830,phrog_1 APIS263 0 IMGVR_UViG_2831644885_000003|2831644885|2831646193 APIS263.hmm PF10076 MEMSEKDYKEAGLKLLPNGLAWNKWYGVVINKLFGGLAKMWAEIDAEANRALDEMNPQWATIMLPEWEELLGLPECNQTGQTIEERRNAAGYKWHLKGSLNPYFYMEWLSEAFGYEVVIVAYHQHHCLRACNYPLYTRREESRDDVYVYIKSKNPQRYFNVQDRANDPLRIGATNIVECILNKYKPAHVELMFKYEDEEV 4 200 5.1563 23552.81 -4.5 AbiU CLAN013 APIS213 phrog_1887,phrog_196,phrog_971,phrog_790,phrog_67 APIS264 0 IMGVR_UViG_2887198475_000005|2887198475|2887199704 APIS264.hmm PF25187 MPANSIPYELAVIPERSPGPLLRALGARRFDGRTIRFTWGEWTPGWGLVLRLRKWSAVYGGGWSLFVQPGYGKLRVSLPLPRREVKGEGAWGFQADLGGGNVHVQWGYGHPGKVYDLPWRAWRCERHDVLAVGGWVPCPEIFAGRMDNPLAATETHPYRYVTDSGEVQEVTATIAVEEREWRLSWLRWLPWVRRVSRTIEVSFSDGVGEQRGSWKGGTVGCSYEMQRGETPAECLRRMQRERRFR 16 245 9.9695 28010.92 11.0 type III CBASS CLAN007 APIS369 phrog_1210,phrog_1272,phrog_376,phrog_1139,phrog_1506 APIS266 0 IMGVR_UViG_3300020182_000807|3300020182|Ga0206129_1001293418 APIS266.hmm PF26760 MDDYMDIHPQDGRPMTASPSTEGEKMEKTLHNSDISGARQNVKDIKVVGNGDLFALLCKASSEAEGWMKSTKAMQTPMGCVVQVTTQQKGIDGTYAVAEALTFVPGAVIVDDENSGRKLVSSADVSQASSA 4 131 4.5392 13882.57 -5.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_5218,phrog_6005,phrog_4219,phrog_296,phrog_453 APIS267 0 IMGVR_UViG_3300028388_003891|3300028388|Ga0307031_1000188166 APIS267.hmm PF26761 VIQRFTAYRRNISERDTHTDLHKNPDDEPQFEGVVFTDGSVVLRWLTPLRSTSVWPDLRSALGVHGHLEYGTVIEWHDGDPPSEWVGQVNKFLESPSGQEDKSGE 18 105 4.7052 11957.10 -5.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_6435,phrog_4201,phrog_2885,phrog_2998,phrog_4585 APIS268 0 IMGVR_UViG_3300037134_000176|3300037134|Ga0395989_000961_290_649 APIS268.hmm PF21825 MTYTYVILPISPAAYAEIRRKLTDAGYTDQFHDDRDGDGVVIDMHGIALSEEREKTMQQHQQRVVDEKKELDEKSSKLNAFFNTAIFDKLSSEEQDRMHRQYELMVQYSAVLGERIAAF 3 119 4.8061 13821.48 -5.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1762,phrog_3883,phrog_6444,phrog_6651,phrog_4 APIS271 0 IMGVR_UViG_3300010344_000225|3300010344|Ga0116243_100145004 APIS271.hmm PF21825 MPEGEEMFKLHGYSGPCPQPPLLQPHQQRVVAEKKALDEKLIAVLQFFQTPVFDGLSEAERSRLRQQARFMDGYSAVLKERIEAF* 7 85 6.4610 9761.23 0.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_6102,phrog_1041,phrog_778,phrog_94,phrog_5208 APIS272 0 IMGVR_UViG_3300049729_000063|3300049729|Ga0495428_004288_4602_5015 APIS272.hmm PF26760 VIKTRNAKNDAVIKSAGVIVSNQPRKIDNLQQSEVLTELVEETLDAKTVEWSKQKVSDVKVYGDGDAFQLLCEKSSTSQGFVKSTKVCNVTGGCLVQTETQQRNPDGSYVLSQALTYCPGVHIAIDQEPRELLRAIY 3 137 5.8884 15074.06 -0.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1506,phrog_314,phrog_2148,phrog_4201,phrog_8917 APIS273 0 IMGVR_UViG_3300050122_000155|3300050122|Ga0500011_001473_1938_2612 APIS273.hmm PF10076 MAAPNYQASDFLTALQSLMPRGLAWPKDTAAVMAKSMSGLAPMWARHTLQNNNLLVDAFPSTTLQLLPEWESALGLPDPCAGESPTIQQRRAQVVARFTNGGGQSLPYFVQFAASLGYDVSIQEFAPARAGQTRCGSPDYGEAWAFAWAIELPLATVVYARAGLSTAGEPLASWGNAVLQCEMARISPAHTILLFQYEVQIYDNSIVDDFEDPVLTDTGFPIII 56 224 4.2248 24257.54 -9.0 AbiU CLAN013 APIS213 phrog_469,phrog_3573,phrog_5298,phrog_21,phrog_325 APIS274 0 IMGVR_UViG_2648501787_000011|2648501787|2651263021 APIS274.hmm PF10076 MAHSVTEWLTALQQVMPRGKAWPRDNDADLNRFLRALAARLTRIEYDASRLHVEMRPETTLQLLPEWEQYLALPECGIAATTTEARRRAVVEKYRRKGGLATWQIEAAAAALGFTIKVTAVLPHHCLRDCMYPLHPARYRWLLKVEVQDSDAGRFTCVDDVMTPLISGRTRELECLLKNYRMGGTGYEFFYTDGNDEAVSGPPLSVNRIQIQAGYKPEEPLTVNRAGLHFALVPALTGKPLTINRINFYAARPMAEPLVLSRAQLHSGLPTTDPMTMNSAQLQTGYRTRSPVPVNSMQLQTASRTREPLLINHPGLHFAVVTSE 4 324 8.8370 36375.82 9.5 AbiU CLAN013 APIS213 phrog_4372,phrog_5218,phrog_684,phrog_5313,phrog_551 APIS275 0 IMGVR_UViG_3300042279_000302|3300042279|Ga0451663_004032_5520_6104 APIS275.hmm PF10076 MRATIDQYLNNLRSLMPKGKAWARENEAGLTRLLRAFATPLMRVHNRAVDLIDEVDPRTSVELLPDWERVCGLPDPCSGQPESLAERRDQVVAKLAARGGQSIPFFVELASNLGYVVTITEFRPFRCISKCNDPLTQGDWRFVWQVNAPAETIRTMTVNSGCSEPLRTWGNAPLECNFNRLKPAHTKVIFTYGA 71 194 8.2413 21847.05 4.0 AbiU CLAN013 APIS213 phrog_2979,phrog_102,phrog_1218,phrog_1098,phrog_3520 APIS277 0 IMGVR_UViG_3300024518_002726|3300024518|Ga0255048_1000002798 APIS277.hmm PF25188 MDVIYKLVEISLMVIIGGPTYKNPREMITVTKVLNEIDRGAYIYRKEIWDDGVNPPTEMKSAYNREGHYIGPSSYGQFLKKRGIVVDIQRPRPDAEGTTCAVGFNPAEQKWYGWSHRAIFGFTIGSTCKKGDCHYVPRDKDDFVQDMIRFWSDPGHINVRTGREEVIDDMFGIHIEWEYDDTSPNEKIRGTIGGAFAVYPVSEDYGKGEWTATTLEEAKEMAIDFAKGVS 6 230 5.0303 26136.50 -5.5 type I Thoeris CLAN016 APIS240 phrog_2,phrog_454,phrog_1293,phrog_5218,phrog_1082 APIS278 0 IMGVR_UViG_3300033120_000539|3300033120|Ga0364481_1001368 APIS278.hmm PF25187 MTETIESIQIEQAAWCEGWRPSPLWLRLLGGPRSFYASRERSYRMAWGELSLRPCGFAIVVGAYDTAHLRIAVGLGQAFIRLPFLDKAMVRGFGDSPSFGVSADATTLHLNWGKRSKVHWWPWTLHHIMTEGLGADGAWFHAHPLRPDLAPGSCPPSWSAEYPYHYMLDSGEVQAVTATVTRERATYGALWFGRGPISRALRSIFPKKVFDGIDIKFSDEVGSRRGSWKGGTIGCSYEMRPGETPRHTLNRMQAERRFR 3 259 9.7735 29213.34 12.5 type III CBASS CLAN007 APIS369 phrog_1535,phrog_4197,phrog_708,phrog_1272,phrog_588 APIS280 0 MN094788_00234 APIS280.hmm PF21825 MAPHQQRVVSEHSELMERLTKLKAFFETQLFKDLPLADQELLETQAFFMDRYADTLAERITRFGV 20 65 5.0210 7688.82 -2.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4271,phrog_4211,phrog_7158,phrog_1396,phrog_7257 APIS284 0 IMGVR_UViG_3300029925_000066|3300029925|Ga0315560_10010823 APIS284.hmm PF26760 MKTLDIDAVKTAKDNIKDIKFFGDPHTFRLISKASSKEEDWMKSTKAMEIEGVGCVIQVTTQQGDNVAEALTFVPGVRIEEYPNGIRRIMPIPHPSGYPAGFVPIKDRIIG 6 111 7.1659 12317.24 1.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_6444,phrog_6616,phrog_56,phrog_6102,phrog_973 APIS285 0 IMGVR_UViG_3300035698_006142|3300035698|Ga0374944_618060_14097_14456 APIS285.hmm PF26760 MSGTRNRKKKGNLIMGEQTKKTLGNSDINGTHKNVRDVETFGNCDMFQLLCKASSENEGWMKSTKAMEVEGVGCVVQVTTQQRNPDGSYAVAEAVTFVPQAVIVGDAENGRKLVWVNCA 59 119 8.1240 12954.71 2.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_2229,phrog_2885,phrog_1068,phrog_2488,phrog_778 APIS289 0 OY979482_00065 APIS289.hmm MYELTKVYKTKAMAVNAIRMTATNKGWKVLSTDDAAQHVWPQAEGFTVNNTSLNLALGFISEADADALEQRVPAGSVTNGEALEAVNAALSEQAHKDAKAPKLQAPSKLEESKKAAKAAKVAVKTKSDVNPQQAEARATRCDVVNGARRPLRGKTLLVWETTEMLAAGPNGIPTLKEVYEELCRLEPGFNKTTAGIQYYACRAYNGWESKK 3 211 9.2973 22896.04 6.0 BREX type I CLAN050 APIS244 phrog_202,phrog_5493,phrog_100,phrog_4492,phrog_3148 APIS290 0 IMGVR_UViG_3300048837_000312|3300048837|Ga0498921_0055479_5217_5573 APIS290.hmm PF26760 MNKSFGTTSPKQAKENTPDIKTFGAGSWVCIEKAWSDEEGWMKSTKALEILNAGCLVQVTTQQKNPDGSYSLAEALSFIPGIVIDTNQFGHSYLHKRISLVAYAPPPSEPYPQQGQQVG 3 119 6.4503 12988.63 0.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_381,phrog_8308,phrog_3209,phrog_4286,phrog_6782 APIS291 0 IMGVR_UViG_3300050508_000042|3300050508|nmdc:mga09592_1456_c1_12837_13121 APIS291.hmm PF26761 MNRSATSRLLGHVADELMWADGREFDLVRYKDVSEVSGTGIVADGIEYTDGSVVIAWHGEHPSTVVWRSIEDAIAIHGHGGLTQIRYRDTGGST 5 Type I CBASS CLAN002 APIS287,APIS361 phrog_6102,phrog_6444,phrog_6705,phrog_1297,phrog_3242 APIS295 0 IMGVR_UViG_3300037332_000228|3300037332|Ga0400270_000075_15112_15615 APIS295.hmm PF25185 MTKLDWAKTELEFAGYPANDPEDGPNKWLREGTLELLEVFSTQGHSGSSAPFALNLFSKLAAYKPLTPLTGNDSEWMEVSDDGICQNVRDGSVFRGADGQAYWLDGVVFWEWHSADDMYDGEPFKTYFTSIDSHVNIEFPWCQPESSEYKFRPSDEYPNEQLTQVGE 44 167 3.9651 18966.75 -17.5 type I Thoeris CLAN014 APIS351 phrog_6088,phrog_3964,phrog_906,phrog_1139,phrog_877 APIS296 0 IMGVR_UViG_3300048930_000528|3300048930|Ga0498917_0001531_44018_44671 APIS296.hmm PF25186 METKRYESMPGNHISTACKEAVEMARRDSCNVEFDFNEITLTAKPDTDPAALAQSYSDECERRHRAYTASPEYKARQEEAERKERERKAKVDAVLADAPANMTLRDPEGWKKACDANQDPYGGAVMTYAERWARMMEARMAKGERIADIAEECSHLANEEGITGFMYGAAVMTLSSVWIHGEALRLWHNLKYQLRDEGKRANDNGGVLNPAVLRMGE 12 217 5.2418 24483.44 -4.5 type I Thoeris CLAN015 APIS258 phrog_2220,phrog_1200,phrog_325,phrog_5311,phrog_21 APIS298 0 IMGVR_UViG_3300017722_002988|3300017722|Ga0181347_100000376 APIS298.hmm PF10686 MRVLVCGGRDYTDKEHVYSILDQCYSQNTKLEIISGMARGADRFAYEWAKERGVKCFEFPADWDRYKKAAGPIRNQQMIDEGFPDAGIAFKGTTGTADMVRRLKANCIPVLDLRSHSPKPLPERVVRHGIMKVSREYDPQSGPRD 3 145 8.8034 16495.84 5.5 AVAST type III CLAN012 APIS354 phrog_1216,phrog_2439,phrog_256,phrog_7187,phrog_4740 APIS299 0 IMGVR_UViG_3300005096_000095|3300005096|Ga0072503_1327469 APIS299.hmm PF26760 MTNEPTMMPGMTEPVDSDVTLKTLYDSDADASVTLLEDEQVIVRADGYFKTLHNSDADGTTKNVPDVKFVGNGDMFQLLCKASSESEGWMKSTKAMQVSKAGCVIQVTTQQRNSDGSYSVAEALTFVPNVRIEDDENGGRRLTG* 3 144 4.1884 15650.39 -9.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1068,phrog_2276,phrog_8917,phrog_794,phrog_10818 APIS300 0 IMGVR_UViG_3300007714_000005|3300007714|Ga0105661_1000004118 APIS300.hmm PF25189 MKRGKQFMRNKLNMNHILSICERDNEGYLIPFMKAQVAVDELCRYFLGDDWYDDSGATHPEQINTNIVAEIERNYKGVKLGWFKRRKIDTDYIWNICDSEGWEGCILSPPMKAQVAVDELCRYFLGDDWYDDSGATHPEQVNVKIVCEIEKRYKGCKCKKTK* 4 162 6.6774 18987.72 0.5 type II Thoeris CLAN034 APIS270 phrog_617,phrog_2263,phrog_4454,phrog_2488,phrog_428 APIS301 0 IMGVR_UViG_3300025875_000001|3300025875|Ga0210040_10000148136 APIS301.hmm PF26761 MRTVRLFNLVRSEDKSGVSGTGVVAEGVEFSDGSCVMRWLSDKTSTAIYASVERLISIHGHEGATRVQYADSMVFDDEHGQWWLTDSPVDVVN 4 93 4.5679 10293.44 -4.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_1762,phrog_8470,phrog_8323,phrog_5313,phrog_907 APIS302 0 IMGVR_UViG_3300027652_000006|3300027652|Ga0209007_100026531 APIS302.hmm PF21825 VDSSEKTNPIPQYRSHKIVGTDSTTDENPIVFIGFGGKWEPVKMSLRGKPTPDVGWYFVMYPDGYTSFSPAKSFEEGYTLIPASFKDRVVAEKAELDEKIGKLQTFATGPIFDKLPPEEKGRMHNQFSVMQDYSKILGERIAAF 3 144 5.7865 16203.38 -1.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_196,phrog_4406,phrog_5313,phrog_412,phrog_430 APIS303 0 IMGVR_UViG_3300024348_001821|3300024348|Ga0244776_100291963 APIS303.hmm PF26760 MNAKKYTKKPVTIEALQWTGENVSEIFEFCSMSYRAIINPETSEMGLIIQTLEGPMTASIGDYIIKGIKGEFYPCKPDIFALTYDDAALTEVKPGKSLHNTTANGAKKNVKDIQFWGDGDTFRLISKASSESEGWMKSTKAMPAGNSVVVQVTTQQRNPDGSYSIGEALTTVPDAIISEYLDADDKVVHRTITQRAVIDAGKVAVRHKIEPITFAEDGSK 12 220 5.3775 24102.35 -2.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_7449,phrog_1538,phrog_104,phrog_2220,phrog_4406 APIS304 0 IMGVR_UViG_3300034101_003818|3300034101|Ga0335027_0009052_4300_4641 APIS304.hmm PF26760 MGILSKTKTEEVVTKTFGNTTSSKASENVKDIVFWGNGDTFKLISKASSQAEGWMKSTKAMEIEGIGCVIQVTTQQGSNVAEALTYVPGVRIREIKENGEVIAREIINRFIED 120 113 5.2203 12387.09 -1.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_81,phrog_6444,phrog_5667,phrog_36,phrog_863 APIS305 0 IMGVR_UViG_3300037420_000483|3300037420|Ga0395906_0002375_7893_8459 APIS305.hmm PF23791 MTLIPPPPTVSTADIHRWCELKKQLDVLKFQESELRKKLFGTLIVNPKEGANEYALPDGYVFKATHVITRTVDQVQMSVLQTTKVRDGLHILRAAGYPDDQIAKMDPDAMLFTVLGLSVDKLIKFKPELSVAEYRKLTAEQTAVVNMFIDSKPGSPQCKLEPGKELKARLEAAAAPQAPSLVPPPPVK 3 188 8.9740 20786.32 4.5 Sir2-HerA,DUF4297-HerA CLAN032 APIS345 phrog_4197,phrog_2335,phrog_1506,phrog_4412,phrog_1056 APIS306 0 IMGVR_UViG_3300042112_000578|3300042112|Ga0451517_0002316_1017_1220 APIS306.hmm PF21825 MALQPHQIRVLAEKDELDQRLQRLVAFMNTATFTELSEAEQARMRRQQDLMAELSAILGERIDAFSA 3 67 4.7023 7703.81 -2.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_317,phrog_2249,phrog_121,phrog_645,phrog_3767 APIS309 0 IMGVR_UViG_3300009149_000210|3300009149|Ga0114918_1000077326 APIS309.hmm PF25187 MKDYKPLRFGKKKNFKLEIYPTWGFYIKYLTEGYDDYGYPLFIFQFIFGSFYLTLPWKHNIKVEGGHDAPSYGITYHMSAFQIYYGKKIKFIHMPYSYDWVRTSLFLKDGTWEHEVKGSRKTFYEEPWLSKQWQITIPYQHTTPNKEVIDLYITCHITEREWRQKWLKWTKWGAKIKRTVDVEFSDEVGEGRGSWKGGVLGTGFDITKTGKIEDGLKIMEKKYDMFSIEWERHKKIKQIIKK* 6 242 9.8634 29176.70 16.0 type III CBASS CLAN007 APIS369 phrog_1156,phrog_239,phrog_305,phrog_1469,phrog_2392 APIS310 0 OQ221560_00084 APIS310.hmm PF21825 MSDFKTRLVEEQVQLEEKLNKLSSFILSDNFNKIDDVQKALLQVQATAMNTYNQCLKERLERL 55 63 5.0000 7419.51 -1.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4352,phrog_4523,phrog_841,phrog_2439,phrog_880 APIS311 0 MW677523_00062 APIS311.hmm PF10686 MRIIVCGGRDFSDREALFRVLDDLHSTHGFTLLVHGAARGADRLAGEWAEARGVTCKPIAADWQMHGLRAGPVRNREMLRTCKPEIVVAFPGGSGTEHMIEISRKAGVTVHVIEGHQEKTSRADRIMAQTPRYEGKI 347 137 8.7918 15142.38 6.5 AVAST type III CLAN012 APIS354 phrog_6102,phrog_1591,phrog_704,phrog_256,phrog_2376 APIS313 0 IMGVR_UViG_3300026519_000116|3300026519|Ga0256839_100469110 APIS313.hmm PF26760 MDYTKKPVTIQAIEWTGDNADEVIDFTDGAAKKKGKGLIVQTLEGDHKASKGDMIIRGVHGEYYPCKPDIFAETYDVGATEPHASTDLVVVGNGDAFQLLCKASSQAEGWMKSTKAMPVAGGAIVQVTTQQRNPDGSYAVAEALTYVPGAKIVDDVNSGRKIAGGRA 4 167 5.2440 17675.91 -2.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_91,phrog_85,phrog_684,phrog_3141,phrog_3222 APIS314 0 uvig_584211_90 APIS314.hmm PF25189 MKNKVDTEHLLSLCEPGDYGIFAPPMKSQVALNELCRYLLGDDWYDPSGATNPEQVNTAIVYEIERHYKRNPWRVLL 6 77 5.0050 8924.15 -2.0 type II Thoeris CLAN034 APIS270 phrog_2979,phrog_9306,phrog_958,phrog_384,phrog_5043 APIS316 0 IMGVR_UViG_3300025162_000075|3300025162|Ga0209083_100067022 APIS316.hmm PF25187 MKEKKTYRTKWFDFTPDWSGFQFRYQLSGYYDTKPVLQIYFIWGKLFLYMPWVHYKKVEREKTLKEKRKDKLNSISGKKIEKKVYKKETYDECDPPQYGVYFYMNQFGINYGKNTKLYDLPWTLDWIRTSALKIDGTWEHDTKKYKNRNFWDKDKWYGILFSETYPYLYITNGGEAQHCLATIRVEEREWRWKWFKWLKWTRKIHKCIEIEFSTDIGERKNSYKGGCTGCSYEMKKNETPFQTLKRMEKERRFR 9 254 9.9829 31475.20 22.0 type III CBASS CLAN007 APIS369 phrog_1533,phrog_7449,phrog_1200,phrog_1272,phrog_104 APIS317 0 OQ995431_00080 APIS317.hmm PF26761 MRRFNLVRNEDETGVSGTGTVAQGIQFDDGTCAMRWLTAKASVAFYDSIQDLEEIHGHGGKTVIEWIDTVNV 55 72 4.4437 7958.85 -4.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_7257,phrog_123,phrog_976,phrog_695,phrog_370 APIS318 0 IMGVR_UViG_3300025896_000477|3300025896|Ga0208916_1000118515 APIS318.hmm PF21825 MEWTPHQHRVLEEQKQLGDRLGALRAFLDSQVFASLPEAERYRLRRQAEVMAEYSRILAERIAAFPRPDDQPAPVPAPTVLSRAALAVISEAVQAYLAQLENREPPPDQSPVDVIQAASQHVMAQRGIAAPSDLREVCTQVLQHYLRKEVR 3 151 6.4881 17040.41 0.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1980,phrog_52,phrog_4704,phrog_56,phrog_530 APIS319 0 uvig_235669_80 APIS319.hmm PF25186 MQTYFISQLAIFQKTTIIKEEIIMATQVSFKDVFSCDGSLCFRFDDGEAFSVKSLNNGIMVIDPICGCTIHSFAKNVAGCFEEGSNFNGLKAIEFEFNGAYVSATAKNATPEKIVQLWNEKMEENRIKYEKEREEYMKTPEYRAERAKALKVGYRRQNVEELVKHSMQTEELQFKDEEARKVWDNFVEVNSKNGYSACVVRYAEYWAKFMQYLMAKHEGVTVAKIADKASHAADIDGVTGFMYGCAVNVLSQVWKHGEELRKWHNKEYDYEGDGVVNPAVLTVSVG 4 286 5.4759 32623.03 -4.0 type I Thoeris CLAN015 APIS258 phrog_7449,phrog_84,phrog_2392,phrog_612,phrog_6 APIS320 0 IMGVR_UViG_3300006190_000247|3300006190|Ga0075446_1000239514 APIS320.hmm PF26880 MKKIAIFDLDGTVIDSDHRTPNKADGTLDLERYFELKTRENIFRDTVLPLAERMKEMYDSGDWHIVICTARDMDQNDFDFLADNNLKFHECFNRSNIRKKYHWGLPDAQYKTKQLKKYKYTRYVFFDDARPIIDTFSTYPNVDMIDATIENKRLKYGT* 4 158 6.8647 18885.39 1.0 Kongming CLAN021 APIS279,APIS286 phrog_2662,phrog_6090,phrog_67,phrog_4871,phrog_3956 APIS321 0 IMGVR_UViG_3300028776_000008|3300028776|Ga0302303_1000009918 APIS321.hmm PF26760 MDKVLDITDIEQAKAQISDLKTFGNGDLFRLISKASSKSGGWMKSTKAMQIDGIGCVVQVTTQQGDNIAEALCFVPGVQICTDGTNLYLGPIPLQPFIGEAVLYGGCCDRGQFQAL 4 116 4.4552 12375.24 -3.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1068,phrog_6651,phrog_345,phrog_1328,phrog_88 APIS322 0 KM378617_00033 APIS322.hmm PF01227 MKAVVTFNQLNVLAMGVAQTMHQLGACKIYAVPRGGVPAALAVQRHDNNFRLVDDPEEADVFVDDIIDSGATMQRYCEQYPEKPFLALVDKTEGMYPDEWVVFPWEVREETSEETVEDNIRRMMQYIGEDVDREGLLETPARVRKAWEFWFKGYQQDPAALLKVFKDGAEKSDEMVVVKDIPFFSHCEHHIAPIIGLATVAYIPNGKIVGLSKITRLVDAFARRLQVQERLTDQIAQTMVDELEPLGVAVQIRARHLCVESRGVSSLGQETITNSLHGVFKEQSACRAEFMTIAKSDKAI 115 300 4.8371 33816.69 -9.5 restriction-modification (RM) CLAN035 APIS281 phrog_708,phrog_2,phrog_84,phrog_6128,phrog_3904 APIS323 0 IMGVR_UViG_3300037420_001065|3300037420|Ga0395906_0002229_11978_12487 APIS323.hmm PF21825 MSTFIKPTVGRVVLLMLGTAAPLGFAKPGDGLPCAAVIAHVHGDRCINVAAFDANGVPRGFTSVTLLQGDDVAPEGVMHAKWMDYQKGQAAKAEQLEAKLATAPGLQPHQQRVVAEKAELDERLAKLRGFLTGDVIKTLGEAEQMRLTTQASCMQEYSDILAERIAAFA 4 169 6.4785 18029.83 0.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_678,phrog_177,phrog_988,phrog_2488,phrog_487 APIS324 0 IMGVR_UViG_2721755500_000002|2721755500|2722785657 APIS324.hmm MNNIKQAIIKLETILENGNEKENRLFVKYNTIKNILDLLEKDQELKIIEMKVELNGVEDSIENATLLEKRLSEAKSLAEDLASTINSLEIKVNSKEEIREAEKSIKLRHFATPSTDF 8 117 4.7445 13429.38 -4.5 TerI CLAN053 APIS265 phrog_1775,phrog_5218,phrog_6005,phrog_3883,phrog_864 APIS326 0 IMGVR_UViG_3300032272_000001|3300032272|Ga0316189_10000012175 APIS326.hmm PF25188 MKSKLLNKRLGLKGVYNIHDELWWHSELEEPTEITYCYTPGGDKIGNVVDTAYICNDRGIVPRPRLDADVGEGKQCRIGFCEKEQKWYGWSHRAMFGFGVGSSVVKGDCAYVGATPEDLITAHEDFWGDLSPERKKQARDECQILPDRSGIRILHTPVMIAMANSLDDVVDPDLDLSELEEIDIHEENPYTIVKCGRGEWEAKTLEDAKVMAQDFAAGVA 3 220 4.5456 24689.86 -12.0 type I Thoeris CLAN016 APIS240 phrog_1101,phrog_1469,phrog_1887,phrog_4372,phrog_29 APIS327 0 IMGVR_UViG_3300043421_000048|3300043421|Ga0451740_0000796_5130_5402 APIS327.hmm PF21825 MLPHQERVVTERAELSEKINKLLTFLTTDTFKSLPEREQYLLNRQVSHMGFYLDTLDERIALFNQPAEPDFLAGSKACDLTGDGTCEACQ 4 90 4.4790 10250.59 -5.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_5749,phrog_8917,phrog_6518,phrog_7507,phrog_635 APIS328 0 IMGVR_UViG_3300049491_000066|3300049491|Ga0499963_000568_14385_14759 APIS328.hmm PF26761 VNVPRTFTLVRDEDVTGVSGTGVIAHGVEFPDGTVVLRWVGEHASLTVWSSLDHAITVHGHGGRTRFVWATDGLDLPGIRRRLTDTQQAEVEQWADKASVIDRSQLDVGPLLEFAENLGGIDRG 3 124 4.8732 13482.02 -4.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_5749,phrog_2662,phrog_2998,phrog_45,phrog_1146 APIS329 0 IMGVR_UViG_3300042350_002569|3300042350|Ga0453929_0000516_19038_19568 APIS329.hmm PF07603 METKEIVELMLKSSDHNPYTGMISKKDNLIAAIDLAKLCKDFADKGQMDEAMNIESEQWVVVISELEGMSLNCESLILKQQFNFEIHPDELGKMTWNEAVEAVKKLGDGWRLPTILELHLIYNSELKDKFKTDDYYWSLSEFDSSITWFFYFFNGFSNYNYKDYTYYVRAVRDLTI 4 176 4.3874 20698.52 -10.5 type II Thoeris CLAN033 APIS242 phrog_1171,phrog_992,phrog_719,phrog_4412,phrog_3141 APIS330 0 IMGVR_UViG_3300042542_005523|3300042542|Ga0453075_0000002_1177_1938 APIS330.hmm PF25185 MKVTKVNNYENWLLEREILSLLIKHLHEGCTMLPIAHVINEGTMSKADYELDRLLKRAEAKGETPLVKDFVPEIKSLVQKFADSGQSGASAPYTSGVIVSVIQKLLNQEPLGGIEGTEDEWVDIGKEAGETPGTMFQNNRLSSVFKDADDKPYYLDAIVFKDADKDYSFTSGSIDLPGLADDAGGGKVGSAQYIKTFPFEPKTFVIDVISTEYHKNEDGTLVPQDGGGWWESVVKDPKQLEEVWQHYDRKTMK 10 253 4.5730 28178.76 -11.5 type I Thoeris CLAN014 APIS351 phrog_332,phrog_457,phrog_6005,phrog_15,phrog_3336 APIS331 0 IMGVR_UViG_3300046528_000074|3300046528|Ga0495642_0000022_8054_8545 APIS331.hmm PF21825 MKFRKKPVVIEATQWFKDGDHPAVRAWDKGGHVYPNGTPLIDTLEGELRVSPGDWIITGVKGEHYPCKPDIFAATYDPADQRPALGAAHPGYSSMQPHQQRVVDEKAELDERLAKLVDFTRTSIFAGLDAEERNRLDQQAATMAMYSDILGDRIAAFASATAA 3 163 5.6024 17996.26 -2.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_262,phrog_864,phrog_3869,phrog_164,phrog_5749 APIS332 0 IMGVR_UViG_3300046804_000918|3300046804|Ga0485268_005100_2621_2824 APIS332.hmm PF21825 VGGRYQPHQERVIAEQQELQQKLDKLNEFMKGEIYAKLDQQSRELLFQQSGAMQQYNSILLQRIQLF 4 67 7.0075 7982.09 0.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4197,phrog_1041,phrog_7507,phrog_3171,phrog_2331 APIS333 0 IMGVR_UViG_3300021440_000023|3300021440|Ga0213919_100006562 APIS333.hmm PF21825 MQKTTIDKMVDKFLGWKLPKDFGPDAGISFKPTKPYEGDELGNSWWPVGTNLLTADQARQMFEHCMDEEPCTSQQPGPHAALPPHQQRVLAEKTELDEKATALSNFIGHSAIFETLDAAEQERLKEQNDVMWQYSEILGKRIAAF 19 145 4.6983 16406.51 -6.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_4747,phrog_168,phrog_7,phrog_1297,phrog_1283 APIS334 0 IMGVR_UViG_3300025679_000024|3300025679|Ga0207933_10012717 APIS334.hmm PF21825 MAPYQQRVVVEKQELDDRIAKLDAFIKGNLFEILHVQERERLIRQLVLMVKLSAVLDERIVYFPKA 20 66 8.5696 7824.27 1.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1847,phrog_476,phrog_9148,phrog_252,phrog_3171 APIS335 0 IMGVR_UViG_3300026484_000394|3300026484|Ga0256837_100005983 APIS335.hmm PF26760 MGFFRSVKTVNAVPMPYHEAGQAGLVRDYSAEEKQVNGYKVVYEDGYESWSPAEAFEKGYMELDPEMGIPKKTLGNTCSNGTRENVSDVIFWGNGDTFKLISKASSEKEGWMKSTKAMQCGSSVVVQVTTQQRNPDGSYAVAEAVTTVPNAVIFEHKEDGKVISRTIA 7 168 5.0358 18447.68 -3.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_1538,phrog_1497,phrog_3883,phrog_45,phrog_3964 APIS336 0 OR243293_00070 APIS336.hmm PF01227,PF14489 MPNSKPNANFLLNNGLKNTSPVPRSYANGVSANQATIANNVETLLQVICGGSDGQTLRSGLLETPNRVAKAFTHWFGGYDIDVPALFKQFEDGAEGCNQMVIVKNIPFYSHCEHHLAPIIGTATVAYIPNESIVGLSKLNRVVDAYARRLQVQERITNQVADAINEHLSPLGVGVKLTARHLCMESRGVCQQGHVTVTTALRGVLLTDPSARHEFLSECK 143 220 7.7056 23890.21 5.0 restriction-modification (RM) CLAN035 APIS281 phrog_239,phrog_3547,phrog_2335,phrog_877,phrog_3883 APIS337 0 IMGVR_UViG_3300028483_000017|3300028483|Ga0233377_100035333 APIS337.hmm PF26761 MTRTFELHRDTDVTGYSGPGVVADGAVFDDGVTVVRWRGEHRSTVAWPSVEAAIAVHGHDGATRLVWTDQVEPTLAEHIVVDRRTGTLTIDDRLFPWAMAADGPRLATDQPGEIVVWVPVLAMGAVSIPAPGERRAQGGRP 3 141 5.1566 15201.09 -3.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_11,phrog_45,phrog_148,phrog_8841,phrog_3004 APIS338 0 IMGVR_UViG_3300028666_000028|3300028666|Ga0265336_10000070144 APIS338.hmm PF26761 MKGDKVKPFYLLRKEDLTGTSGIGVVAMGAIFPSGQVFLEWIASNHVSWNMFDNIEDVKSINGHDGRTEVIMGNPEDKIKKDRKKDK 5 87 8.5753 9780.23 2.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_4191,phrog_4286,phrog_8841,phrog_601,phrog_7507 APIS339 0 IMGVR_UViG_3300035701_000757|3300035701|Ga0373625_0019666_9851_10108 APIS339.hmm PF26761 MTKPKEGKIKGAKITGRRFWLMRTKDISQVSGTGCVADGIVFPGGVSVLRWRTAGGSTAVYDNIENLERIHGHDGKTIIKFIDKP 49 85 10.7226 9313.82 8.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_8735,phrog_8323,phrog_6705,phrog_778,phrog_2631 APIS340 0 IMGVR_UViG_3300046523_000205|3300046523|Ga0495644_0000042_40097_40531 APIS340.hmm PF26761 MISRFTAYRRNLSELGVHNEKQANPDDAPQFEGVVFSDGSCVLKWLTASKSVSVFSSLSDMLQIHGHPEYGTDIQWHDGPMPDEWKRQLVAHGEHRQSELYAAGFERCKVVVHNDDAGNIVSIRVQSPSGAVDKQVYPPETCHE 3 144 5.7909 16134.93 -3.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_1762,phrog_1497,phrog_33,phrog_3830,phrog_257 APIS341 0 MGV-GENOME-0345538_120 APIS341.hmm PF21825 MSLGRLCKTLKYMETFIQRIVDEKAELDERAGKLGDFVKSEKFHSLDSEIQSLMVKQYDVMKRYSVILGKRLELLDA* 18 77 7.9939 8986.54 1.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_3545,phrog_206,phrog_5156,phrog_5023,phrog_6160 APIS342 0 MGV-GENOME-0245195_22 APIS342.hmm PF25185 MIRVEYSKDLEKAKIANIGIGIDGKKIVRLYEHSNEENYNVGHTTKGTETTDKLMRGRECLVELTFYHNESIDSLIKVLQDIKNADFINKESDPETYKNCKEILEEKNNLVLHAERELEILLNQCEDEEGREMQKHINKDILEVVEIFSNQGHSGFSANYAIGMIEKLLRYEPITPLTGADDEWTKLDYNDDTKYQNKRCSRVFKNADGQAYDIEGKIFSEDGGKSWYQSKDSREYIKFPYRPHTEKIILKAKEK* 3 255 5.1771 29699.39 -6.5 type I Thoeris CLAN014 APIS351 phrog_3547,phrog_326,phrog_4201,phrog_1200,phrog_5311 APIS343 0 IMGVR_UViG_3300001112_000001|3300001112|JGI12322J13274_100000173 APIS343.hmm PF26761 MAEPKRKVELRRFRLKRNEDESGVSGTGYVAEGIKFSDGQCVISWLTDTRSIGIYHSTVEMIHIHGHGGKTVIEWIDAESESGSGTAIEGSGEHAQRQLTKTEA* 3 104 6.3298 11443.72 -0.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_5749,phrog_3336,phrog_3869,phrog_4728,phrog_87 APIS344 0 IMGVR_UViG_3300020576_005179|3300020576|Ga0212216_1000009333 APIS344.hmm PF21825 MLPGNNLRDNEFMRPLEPYQQRVIVEKKELDTKLDALTAFLSSGVIPITENEKNLLIFQHAAMSSYSKILGLRIDAFKPS 3 80 7.0097 9106.57 0.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_761,phrog_1762,phrog_746,phrog_5302,phrog_2331 APIS346 0 IMGVR_UViG_3300037418_002225|3300037418|Ga0395900_0002253_1325_1765 APIS346.hmm PF10686 MAVYCDPSILPSTKRVLVCGGRDFSDAAWLARILDHLHDASPFELLIHGGARGADTLAGNWAKARGVPVQVFRADWDKHGKVAGILRNKQMLEEGKPSLVVGFPGGRGTADMMRRTAEAGVDWLRVSRDSDTRPKDGDAQQGSARE 19 146 8.5449 15836.95 4.0 AVAST type III CLAN012 APIS354 phrog_5616,phrog_864,phrog_2051,phrog_2244,phrog_1047 APIS347 0 IMGVR_UViG_3300005820_000015|3300005820|Ga0078747_1003554 APIS347.hmm PF25188 MDTKVLSVRNYKAGYEIRTQEVTMYDNPPVVMKSAYTPDGDYIGTSRWAHRLIVKRGIKPEKRTPESNVCSIGFCEKEQKWYGWSHRAIYGYGIGDVIEEGSSASTSGWTDEYLAEHPEEDVSLPVGFAAQTLDDAKLMACAFAESVS* 70 148 4.8262 16540.51 -4.5 type I Thoeris CLAN016 APIS240 phrog_326,phrog_63,phrog_4197,phrog_511,phrog_1762 APIS348 0 GQ919031_00095 APIS348.hmm PF26761 MSEARRFHLQRNVDVTGASGTGRVADGVLWPDGTATLRWRGERASTVNWDRIEDAESVHGHGGHTTIVWDDWPTQDVDPDLPCMFCIDGHGAPTRCAWGVRVGPERDIDGQPTQLIVQPTAGQHVAPEDAAWLHRLIHPTA 19 141 5.4458 15515.17 -4.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_8917,phrog_3940,phrog_3339,phrog_264,phrog_5158 APIS349 0 IMGVR_UViG_3300020046_007608|3300020046|Ga0206646_100284710 APIS349.hmm PF25187 MSSTIYRIKRSLRGCKLRPSFGLCLFEETGYCLDLFGFLIALPFLDRWYREPNEIMASWGACYFDRAIMLKWGDWTKFIYMPWSMDHVKREVMQPDGKFVLKDRSYDGDPKDGRWVADYVYRYTLRSGEVQVRIATVYVERGYWHWLLAKWLRLPLWLPVVNMMKQSIDVRFDDEVGERTGSWKGGCIGTGYSMLPGETPEQTLRRMERERKFN 6 214 9.0355 25494.55 7.0 type III CBASS CLAN007 APIS369 phrog_5342,phrog_3573,phrog_332,phrog_612,phrog_8927 APIS350 0 IMGVR_UViG_3300026286_000087|3300026286|Ga0209460_100009060 APIS350.hmm PF25187 MKKLRFAFEREHKGWLLRMWWNWAEVAYLLLALRGLKAEIEFPSDWHEHRQGWVRLGFGLFTLAFAFPWPWVVPDDYQCSGPRFGFCFFQDGLHLSWGKSHGKRDDPFKIIQMPWGWRHRKHEVLTEPEQFPYTYRLRSGEVQERIATVKEERRLWTRPWLPHKRESHYIDISFDKEVGERSGSWKGGVMGCSYDMLPAENPLMTLRRMERERNL 3 215 9.6116 25997.88 10.5 type III CBASS CLAN007 APIS369 phrog_4211,phrog_454,phrog_2335,phrog_1293,phrog_6117 APIS352 0 MT345684_00059 APIS352.hmm PF01068 MKPMLACDADLTKVTWPMLGMPKIDGVRALNIDGKLVARSGKPFKNRENTYFFSTEVLEGFDGEMVAGGITDPNLCSYTTSAMNTIDGEVQCDWYLFDYFGPQVAPTAKYEERLNTLHSIVAKLRKDYPVQSTRISVVPVKILNNQEEAEQYFADNLAQGFEGTILRNPKGPYKLGRCTAREANYLRVKAFADAEIKVHFVQEGRTNLNELKRGNFGQAERSTNADNMVPNGMVGTITGELLQDIVVKDEVFLHKGTVIEIAPGRLTHDERVKYMQDPALMCGKIAKFQYFPIGMKDKLRFPTFQCFRDENDL 5 313 6.4933 35290.41 0.0 AVAST type III CLAN022 APIS312 phrog_1330,phrog_58,phrog_616,phrog_24,phrog_5342 APIS353 0 KT887557_00038 APIS353.hmm PF21825 MTQSYIGTKQILAWEQDRDGQPGYTVKYADGYMSWSPKDVFEAAYLRMGHIGHLLPHQQRIVGEKVQLDDKVDKLSRFLGGEFFRSLESGEQERLTAQLGAMREYQQILAERIAAF 12 116 6.2305 13342.11 -0.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_1802,phrog_7158,phrog_45,phrog_3736,phrog_2051 APIS357 0 KP313531_00008 APIS357.hmm PF27024 MNAIYIMNAIDASKALPICELDKRQGMLIDLLVEMVNSETCDGEITKLNQALEHQDWWTTLKCLTTDAGFKMLGNGHFSAAYSHPLLPNRVIKVGFKKEDSGAAYTAFCRMYQGRAGIPNVYDVQRHAGCYTVVLDALNDCERFDNDEHYKYAEIASDIIDYKSDEHDGLTGWDGEFVETCKLIRKFFEGIASFDMHSGNIMFSDDDTPFITDPVSFSQKKDGGAFSIDPEELIKEVAHQRVIDRAKERKARHVGRLEARKVKRRNRKALKAHQEKHKRMVAAWRMA 3 287 6.8684 32702.22 2.5 Retron-Eco9,DarTG1 CLAN017 APIS010,APIS276 phrog_58,phrog_964,phrog_38,phrog_23,phrog_9 APIS358 0 IMGVR_UViG_3300005918_000040|3300005918|Ga0075116_1000007358 APIS358.hmm PF26760 MSKTLHNSDVSGAKKNVSDLVTFGNGDMFKVLCKASSKEEGWMKSTKAMEIPGVGCVVQVTTQQGDNVAEALVFVPGVKVIIDPSAPSDPNVVTGRALGKT* 20 101 7.8314 10551.14 1.5 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_5939,phrog_257,phrog_2158,phrog_87,phrog_3545 APIS360 0 IMGVR_UViG_3300023301_002375|3300023301|Ga0209414_10067228 APIS360.hmm PF21825 MSDYLSRLKEEEKDLKTKLGKLFDFTQSEKFKTVEQFEESIIQIQLEAMRTYYRCLAERIGDK 5 63 5.1367 7599.71 -1.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_7158,phrog_4201,phrog_1794,phrog_3011,phrog_10401 APIS362 0 IMGVR_UViG_3300010214_000044|3300010214|Ga0136260_10022623 APIS362.hmm PF11195,PF21825 MNFGDAIKELKLGKRLQRTGWNGKGLFIYLVPAASYPVQTGAAKEHFGAGAMVPYAAYLALKNVDETVSTWAPSINDTLAEDWQVVGCTLPGHQQRVLDEKQELDIRITRLDEFILRNALFRELANDEQARMRRQLDVMRELSVILGERISAF* 11 153 6.4771 17278.83 0.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_2231,phrog_372,phrog_1538,phrog_327,phrog_6102 APIS364 0 IMGVR_UViG_3300022822_000327|3300022822|Ga0222646_1010437 APIS364.hmm PF26760 MQIYPVVYQSHKKVIRFVMTRLHYNNYRNWKLPEDEDGSDEGYLVEYLDGSKPNHHAHKGYISWSPKKQFDDGYSVVAENEDKTLSNTDQDGCRKNVSDVVIFGDDLFKLLSKASSVKEGWMKSTKAMDTGNGCVVQVTTQQRNPDGSYSCAEAVTFVASTCISEHKDKDGIVTKRWLESYS 7 182 6.4865 20692.05 0.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_454,phrog_6468,phrog_4406,phrog_2689,phrog_684 APIS365 0 IMGVR_UViG_3300035208_002115|3300035208|Ga0376279_0000001_638747_639460 APIS365.hmm PF25187 MGKNFRIDKYGEPKEYKTKWLTYYPFICGFALIYEKAGYFDARPCIQITLIGQLFIHLPFNSKIYSHKSPKYGIYYHDNGVWFCIGEKNRVVHMFWDKTWVRTSYLLIDNTWFNNNKTNRDDYDMNSLWKLVIKYKYKIENGNSIDVDAVITVEEREWRYKIIKWLKFFPIIHKDLSVSFSTDIGKGRGSYKGGITDISVKYEKGETFQQVLERMEKKYGLYSIDKERMKKIETIYE 3 237 9.5754 28472.85 12.5 type III CBASS CLAN007 APIS369 phrog_10,phrog_1101,phrog_2083,phrog_794,phrog_4454 APIS366 0 IMGVR_UViG_3300042305_001032|3300042305|Ga0453197_0002244_8162_8464 APIS366.hmm MKLEIRKIYKNRKGRLIRITKGAVKIMETKEIVELMLKSSDHNPYTGMLSKKDNLIAAIDLAKLCKDFADKGHMDEAMNIESHQWVVVISELEGMSLNCA 5 100 9.0976 11391.49 4.5 type II Thoeris CLAN033 APIS242 phrog_5218,phrog_6090,phrog_1497,phrog_6435,phrog_9600 APIS367 0 IMGVR_UViG_3300048606_000068|3300048606|Ga0502575_0000227_8169_8681 APIS367.hmm PF26761 MSRLQTAFPDGAAFYWNEDGELCVCWSFDDPATVQDPFAHEPFPGHRELSRIERHMTVSDWVDQNTIAGTDYDVIATGGNDIVTVVLQRPTGGTSNGLTVGRRFILVRHEDVSKVSGTGVVAQGIQWPDGTVSMRWSNPGLPSSFACWDNIEAIERIHGHQGKTEIEWID 4 170 4.4720 18856.90 -9.0 Type I CBASS CLAN002 APIS287,APIS361 phrog_1980,phrog_595,phrog_617,phrog_106,phrog_2998 APIS368 0 IMGVR_UViG_3300044719_000205|3300044719|Ga0466971_0000022_25985_26893 APIS368.hmm PF01068,PF14743 MSFKPMLAAAADLEKLRFPLYASPKLDGIRAIVRDGVVLSRTLKPIPNAHVQRVFGNQAYNGVDGELIVGPPTSKTVYQDTVSGVMSRDGEPDVTFYAFDYLPTDGWTEYPWLHRFTELKRRINYVLIEPLDHWEITNHGGLFGTEEKVIERGYEGLILRHPQATYKFGRSTVNEGYLLKLKRFQDSDAIVLDVIEEMFNGNEATKDELGRTKRSSAKAGKVGKGTMGALKVMDIHTGVEFECGAGFTAEQRAKFYTDPPKLIKYKFFPIGVKDKPRHPVFLGARDPSDVELSTAMRTRLRK 203 302 9.4381 34034.03 8.5 AVAST type III CLAN022 APIS312 phrog_1156,phrog_339,phrog_23,phrog_305,phrog_202 APIS370 0 IMGVR_UViG_2880141355_000006|2880141355|2880143565 APIS370.hmm PF26760 MKEKDLNISEVRGAKKNISDLQVYGDGDMFALLCKASSQEQGWMKSTKVCNVKGGCVMQVTTQQKNPDGSYAVAEALT 4 78 7.6734 8474.69 1.0 Type I Thoeris,Type II Thoeris CLAN003 APIS288,APIS359,LockinC phrog_3869,phrog_1601,phrog_2076,phrog_1345,phrog_2331 APIS371 0 PQ765507_00081 APIS371.hmm PF21825 MSKEMLGFQARVVEEHDQLVLKINALEEFIKGGVFETLDARQQGFLISQLDAMGAYAHVLATRISYFGE 4 69 4.6615 7761.91 -3.0 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_761,phrog_4033,phrog_6102,phrog_6705,phrog_422 APIS374 0 OQ053201_00023 APIS374.hmm PF21825 MDHFIGVKRVNAKPMTRGEYNAFRGWELPADENGADEGYLVEYVDGGKANTAEYAGYVSWSPRDVFERAYRRLPALDHLAPHQQRVVIEKDDLDERTEKLERFIPSQKFHELPTEERDRLRRQYHLMVALSAVLGERIAAF 30 141 6.0131 16406.43 -1.5 Thoeris CLAN001 APIS024,APIS029,APIS211,APIS245,APIS283,APIS315,APIS356,APIS372,APIS373,APIS378 phrog_67,phrog_2335,phrog_1469,phrog_1506,phrog_3336 APIS375 0 PP816325_00034 APIS375.hmm PF01068,PF14743 MQTPFKSPMLAKDFDESKLSFPLVASPKLDGIRAVVSSHEGLGYPLLYSRSGKSIKNKFIQSQLCVAPFYLTGLDGELVVGPYNAPDVYAKTSSGVMSEKGEPDFTYYVFDCYGDVDDGAYASRCFELEDRVNAFNTANFGERMGRVKILEQTLILNAQELEQYEADMLAAGFEGVMIRNPAAPYKFGRSTVKDGALLKVKRFAHDEAEVVGVEEMMHNDNEAFTDELGRTKRSTSKENLRPSGMLGAFICKSPKWSHTFNVSAGSLSHDDRRLIWEQSQINKKTPWPTLLRFKHLPHGAKDRPRHPLFAGFRDPDDMSNVK 5 322 6.9055 36081.93 2.0 AVAST type III CLAN022 APIS312 phrog_167,phrog_2206,phrog_16,phrog_310,phrog_991 APIS376 0 IMGVR_UViG_3300042887_002430|3300042887|Ga0453665_0000091_41858_42400 APIS376.hmm PF25188 MYLNKISDVKPIDMDDGTEKLAYFSKYDGSYITLTGLENSVRYLMKRGITKDLAHGVGYSPRKKKWYGWSHRAIYGFKIGSTVTQDDCAYFPTNKQDYIDSYIEFWTDVDNHKTVSVEDITEEGFNIIFNYNDTVPNESLKNKISSVYQEFPKQWGRGEWTAKTMDDAKQMAIDFCEGVS 4 180 5.0625 20822.30 -3.5 type I Thoeris CLAN016 APIS240 phrog_1171,phrog_5311,phrog_2335,phrog_3830,phrog_2871 APIS377 0 IMGVR_UViG_3300045988_038907|3300045988|Ga0495776_168909_14241_14651 APIS377.hmm PF25186 MLRSKQNRKHPGTDEEKEKWIEMKIVKGKEQEYKDWYEKNSDPYGRACFAYAERWAGMMEEKIKASEEDEMKVIVDNAKRLSYESDTEGITGFMYGVAVSILSQYWEYGEFLRKWHNKEYGYDGDGVVNPAIITVG 329 136 4.9286 15955.93 -4.0 type I Thoeris CLAN015 APIS258 phrog_1293,phrog_8841,phrog_1762,phrog_4200,phrog_1149 APIS379 0 MGV-GENOME-0303377_11 APIS379.hmm MSFNLYLAGSKVNTQNDIIIKRECDVLFSQINDRKAIMKFLEVMSNNKLFIDSGAYSAWSKNKHIDVEDYIKFINDNTDKFTLFASVDDIPGELKRKPTLLEQRESPEKSWHNYLYMREQVRDKDKLLPVFHIGEDFRHLQNMLEATFHGKHIPYIGLGGTVGLASSVKEDWYKQCFKIIQQSKNPKVKVHAFGMTNLDILENYPFESADSTTWLMAAINGELCTKYGRICVSSKVQHKVSHYNKLPQLVQRQIDEQCVSYGTSIEQCMENQESRQLYNINYFKDWADNYKYKGNNRYQKRLF* 4 303 8.4285 35508.46 8.5 restriction-modification (RM) CLAN028 APIS204 phrog_31,phrog_4527,phrog_38,phrog_1204,phrog_262 APIS380 0 OX241569_00041 APIS380.hmm MTKFFRHLLLATYKTTTHKYVSDGEMCKIYNVEHSRITNSRNQLIQEGHSFVFKTELTKNNSIRMVFKYVGDTNLGLVCVDEKKDALDGDTLLRLRYRMQSSNVCNLLEALAPLNTKGYWTDLQLSELTGLEVKTVKSCVANVVQHYKLIIHHREHISKREHRIVGIKLPSEIAASTAVKDYSLLTKVFQ 3 190 9.6567 21845.29 12.5 DRT type I CLAN054 hades phrog_1204,phrog_262,phrog_2662,phrog_124,phrog_2276 APIS381 0 IMGVR_UViG_3300045119_000662|3300045119|Ga0467007_0000356_4687_4911 APIS381.hmm PF26761 MDHARRFELHRDEDVTGVSGTGVVAEGVIFRDGVGCLRWLTEWPSSVVHYDRGLESIETIHGHGGKTRIVWIDE 13 74 5.4803 8344.31 -2.5 Type I CBASS CLAN002 APIS287,APIS361 phrog_6005,phrog_6819,phrog_1591,phrog_992,phrog_635