| APIS001 |
Apyc1 |
pyrimidine cyclase system for antiphage resistance (Pycsar) |
CLAN008 |
Bsp38 of Bacillus subtilis |
PMID:35395152 |
SBSphiJ Apyc1 efficiently hydrolyses a wide range of cyclic mononucleotides; 273 Acb1 and 107 Apyc1 phage proteins were identified. |
| APIS005 |
Gad1 |
Gabija |
CLAN009 |
phi3T of B. subtilis |
Yirmiya et al., 2023 Antine et al., 2023 |
Gad1 forms an octameric web that encases the GajAB complex and inhibits DNA recognition and cleavage. |
| APIS008 |
Gad2 |
Gabija |
CLAN023 |
SPbetaL7 of B. subtilis |
Yirmiya et al., 2023 |
Alphafold2 predicted that Gad2 is an enzyme with a nucleotidyltransferase protein domain, suggesting that it inhibits Gabija via a mechanism of action different than Gad1. |
| APIS024 |
Tad1 |
Thoeris |
CLAN001 |
SBSphiJ7 of B. subtilis |
PMID:36174646 |
Tad1 proteins are not an enzymes, but are 'sponges' that bind and sequester the immune signaling molecule (cyclic ADP-ribose or gcADPR) produced by TIR-domain proteins, thus decoupling phage sensing from immune effector activation and rendering Thoeris inactive. |
| APIS025 |
Had1 |
Hachiman |
CLAN068 |
SBSphiJ4 of B. subtilis |
Yirmiya et al., 2023 |
Had1 (Hachiman anti-defense 1) is a Hachiman-inhibiting family of phage proteins. The mechanism of Hachiman defense is unknown. |
| APIS027 |
DSAD1 |
defence-associated sirtuin (DSR) |
CLAN070 |
SPbeta phages of B. subtilis |
PMID:36192536 |
DSAD1 is a phage anti-DSR2 protein that binds and inhibits Defence-associated sirtuins (DSR2). In bacteria, N-terminal sirtuin (SIR2) domains within DSR2 were shown to participate in defence systems including prokaryotic argonautes (pAgo), Thoeris, AVAST, defence-associated sirtuin (DSR), etc. |
| APIS029 |
Tad2 |
Thoeris |
CLAN001 |
Bacillus phage SPO1 |
Yirmiya et al., 2023 |
Tad2 is a "sponge" that sequesters the immune signaling molecules produced by Thoeris TIR-domain proteins in response to phage. |
| APIS041 |
|
|
CLAN008 |
|
|
|
| APIS044 |
|
|
CLAN008 |
Bacillus phage W.Ph. |
|
|
| APIS101 |
ugi |
DNA repair |
CLAN077 |
Bacillus phage AR9 |
PMID: 6776115 |
Uracil-DNA-glycosylase inhibitor. |
| APIS102 |
|
|
CLAN029 |
|
|
|
| APIS132 |
MpaR |
SOS response |
CLAN029 |
prophage of Listeria monocytogenes 10403S |
PMID:31754112 |
MpaR plays a critical role in the de-repression (induction) of the two phage elements and is sufficient for ϕ10403S induction. |
| APIS178 |
AriS |
SOS response |
CLAN047 |
prophage of Listeria monocytogenes 10403S |
PMID:35443160 |
LMRG_02920 (AriS) encodes in prophage L. monocytogenes strain 10403S. It acts as a RecA inhibitor and inhibits the bacterial SOS response. |
| APIS181 |
|
|
CLAN029 |
|
|
|
| APIS192 |
|
|
CLAN026 |
|
|
|
| anti_TerI2 |
anti_TerI2 |
TerI |
CLAN110 |
Listeria monocytogenes 10403S |
Azulay et al., 2025 |
Anti-TerI2 counteract TerI during prophage induction to allow virion production. |
| APIS265 |
anti_TerI1 |
TerI |
CLAN053 |
Listeria monocytogenes 10403S |
Azulay et al., 2025 |
Anti-TerI1 counteract TerI during prophage induction to allow virion production. |
| APIS224 |
acb4 |
cyclic oligonucleotide-based antiphage signalling system (CBASS) |
CLAN093 |
Bacillus phage SPO1 |
PMID:40845805 |
Acb4 proteins bind nucleotide signals and inhibit a broad range of CBASS operons. |
| APIS288 |
LockinA |
Type I Thoeris,Type II Thoeris |
CLAN003 |
B. subtilis phage SBSphiJ |
PMID:41785351 |
act as nucleotide “sponges,” binding 1′′–3′ glycocyclic adenosine diphosphate–ribose (3′caDPr) and histidine conjugated to aDPr (his- aDPr) |
| APIS240 |
Tad6 |
type I Thoeris |
CLAN016 |
|
PMID:39855193 |
Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB |
| APIS242 |
Tad7 |
type II Thoeris |
CLAN033 |
|
PMID:39855193 |
The conserved loop of Tad7 binds the catalytic E99 residue of type II ThsB via a hydrogen bond. |
| APIS258 |
Tad4 |
type I Thoeris |
CLAN015 |
|
PMID:39855193 |
Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB. |
| APIS270 |
Tad8 |
type II Thoeris |
CLAN034 |
|
PMID:39855193 |
The conserved loops in Tad8 occupy the pocket within ThsA responsible for His-ADPR binding. |
| APIS351 |
Tad3 |
type I Thoeris |
CLAN014 |
|
PMID:39855193 |
Tad3, Tad4, and Tad6 each have a conserved loop that blocks the active site pocket and interacts with the catalytic E85 residue of the type I ThsB. |
| Tad5 |
Tad5 |
type I Thoeris |
CLAN052 |
|
PMID:39855193 |
Tad5 can interact with E85 and other active site residues in ThsB but does not completely block the active site pocket. |