CAZyme Information: MGYG000004885_00065

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotellamassilia
• CAZyme ID: MGYG000004885_00065
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1787	MGYG000004885_1|CGC1	193409.34	4.4635

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004885	1991431	MAG	Spain	Europe

• Gene Location: Start: 71527; End: 76890 Strand: -

Full Sequence      

MRKICLFIAALMLTCLAVPLSAAAETKNPQAVSALLDRIGGTGASERFVTVVDETLANGG
KEVFVITAEDGKPCIKGSSTIAVTTGINWYLNHHARINLAWNCLTTDLNAATLPVPAAEE
RHQCTVDYRYYLNYCTYSYSCAMWTEQRWMQEIDWMALHGINMPLMLVGLDVIWHDIYTS
DEFGYTTAELGKYVAGPAFQAWWGMTNLEGHGGPNPEWWYERQRAMCKNMLARMRELGMD
PVLPGFYGTVPSNLATKMAGKEGYTQVNSGGKWQGYNAPGMLSATDAKFQHLAQLFYEAQ
EKVMGKSKFYSMDLCHEGGTAPQCGRPTQAQGIIQAMNANCGEQSVWVIQAWQNNPANDL
LNAVPKGRLLVLDLFAENTPRWGRFGQHDFVYCMLLNFGGRTGLHGRFDNLVNNYNNVLA
AKPSQLKGIGATPEGIETSPVMYDALFELPWTTIDKTKWVKEYATMRYGQENQAAQSAMQ
KLMGSVYNCPGGQQGTSEGVILAQPSLTVNSVSTWSTSSIPYDHTKVIAAADDLLSQAAT
LEGNNFEYDLLDVTRQAITDYAYFLLKQINADYQKGGGTNADFRTRKDCYLELIDDLDEL
LGSHQSYRFGNWTEMARNIVKEPAAQAAGATNTDADWLEYDNLRRQVSTWSDFNSQLREY
SNREWSGMLRDYYKPRWVEFFNALAAGSGTGGLNNAYWYNKGCEWIKNTAGITYTAAPEG
DTKVLAAEKFAKYFFPLVSWNGDKVYVQRHIEQELAAADYTTFAYRGQNYTFAIPEGTTA
QLAVDVNNDGNISADEQSADNTLAIPADAATSNVKATVRISDGTVVSFYIALKDEILNDR
TVSVAVAEGCEGQGTVSIVGAPEGTNSVTNKETVVIKATPAEGFDFKGWTQTIGGETKAA
GTENPLTYYGADEATFTASFVENLWGIPAEENANEHATVENVKSYLSAISYTQGENTTEL
YSATECPAQYFNPTAGNINVARGSKFSVQMTDAGGMKYCYLSAYIDLNRDGDFDDEGELI
EVRGTKGAETGGICNNPVEVLLPYDMPAGLTHLRLRFDGAWKNCIPATGQPAPAKADLTR
MTYEIPVNISEHALGSTHIVIRSCDENMGTVRNITGESGEDISVSAGTEIQPEAFPNEGY
EFVHWEDQYGRVLSTEPAFKIIPAENTTFTAVFKKKLTLVTAGDIVLQTQETLSGDLLVM
GVESGSGVLDLNNLSGIPEGKKLVGFAPGAVSGNKDVKRIVLPEDDIAFDCLLNASFEGA
GTQNDLIRPNTTIPGKGPWTLTIKVATDGSSFNQWGSGLLAVGDNALANSYPGAFQFYLK
AAGTIVMKMNGGGENTFAQNVGSRFTIEMVNDGAGNFNVTLTPENGTPETKTFSNIALND
ITVFSTALPEGVAIEQLNLSDPTLSSQPWKGCTGLEYFVVTEGNPNYTAKDGILYSADGS
RMLAFPEGRLITRYFNLRNLANGKLAYATPLADADGQMTANNGSNRAVLAGEAASTDVPT
ALWTLVPIDNGYKVEHTNSQRFFGGKAGQHNRVEMPTSATQWYGEYTYDGSITAANEYAL
ALKVGTYYVKNSDTGLILEETSAPDNTFLWAVDEVKSIPVTVDDDLWTALCLPVAVIVPS
ADDATVYTVSGVNNGALSLSPVAEGSVIPAGTGVLVSTEAAGTVSFALSYEAITASLSGN
LLAGATLERTGLTAESFYLLGNTAAGTGFSRSTGTSAPANQAYIPTANIGNEAASFLSLD
GTPTGIGTATGDRGKNNGIWYDLRGRRVLYPAKGIYVNTAGEKVFID

Enzyme Prediction     

No EC number prediction in MGYG000004885_00065.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	71	731	2.9e-187	0.9939668174962293

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	2.01e-134	129	453	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	9.69e-69	459	726	1	257	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	4.24e-15	30	114	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.
pfam18998	Flg_new_2	0.003	841	923	2	73	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12583.1	1.75e-208	15	734	10	718
AUI45172.1	2.97e-165	32	721	23	705
QCQ31494.1	1.11e-164	32	721	23	705
QCQ35767.1	1.11e-164	32	721	23	705
QCQ44686.1	1.54e-164	32	721	23	705

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VC9_A	2.16e-104	21	706	163	847	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	2.57e-104	21	706	171	855	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	2.20e-103	21	706	186	870	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
4XWH_A	5.68e-95	25	734	2	712	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P54802	5.38e-94	25	734	25	735	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2
Q9FNA3	4.37e-87	31	734	48	805	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000987	0.961291	0.036851	0.000321	0.000266	0.000249

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004885_00065.