CAZyme Information: MGYG000004876_00215

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000004876_00215
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
659	MGYG000004876_2|CGC1	75439.09	6.4005

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004876	4781463	MAG	China	Asia

• Gene Location: Start: 5991; End: 7970 Strand: +

Full Sequence      

MTIKKFYILCAALFCFMAVSAQQNISVSSPDGKLKFLLKITPETVSYEVNYKKQLLVENS
LLGFNFDNGEFGSNLKSGKVRRKSIDETYELVVGKVSSARNHCNEMIVPLQEKEVPGRLI
NLVVRAFNDGVAFRYEFPEQEGWNSYVMYDEKTQFRLNGNPMVLLMYLPGYINTHEGVYT
HTEYDKIAKKRLIEMPVTLEFDNGIYMSITEAAVRDYAGMYLVKEKNGFAGKLSPRLGQE
KIKVEINEFPHRTPWRVLSVADRVGGLLETNILTSLNEPCKIEDTSWIKPCRTTFTWWNG
NVVPDSTFSPGNNFETNKYYIDFAARNGLDAHGIYGYAETPWYYDDNFNFGWAGPNADIT
RPIPCLNMPRIVEYAKSKGVGIHLWVHWRPLYDKLEEAFGLYEKWGVKGLMVDFLDRDDQ
EMIRIQEEILQAAARHRLFIQFHGSSKPSGLTRTYPNEFTREGTLNYEVCKWDTLVNAGH
DISVPFTRLLAGPTDYHLGGFRALPRSEFKIQYVNPYVMSTRCHMMAMYVVLENHLTSLC
DTPMAYEGQPGFEVLRTVPGTWDEMRVPLAEFNKYVTVARRNGTDWWVGTLNDGTARTLN
LELDFLGEGEYRAEIYTDAPDAEKNPNHLSKEIRTVTRKDKIELPLAVDGGAVLRISKL

Enzyme Prediction     

No EC number prediction in MGYG000004876_00215.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	13	656	3.3e-196	0.9952456418383518

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	3.34e-122	284	559	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	3.42e-80	29	279	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	9.09e-37	562	657	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK86458.1	0.0	4	659	13	668
QUT61529.1	0.0	4	659	5	660
QQA30035.1	0.0	4	659	5	660
QUT37383.1	0.0	4	659	5	660
QPH58760.1	0.0	4	659	2	657

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	2.02e-100	29	656	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	3.08e-99	29	656	20	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQ4_A	6.15e-93	26	659	2	661	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	6.15e-93	26	659	2	661	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	1.69e-92	26	659	2	661	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	3.71e-104	4	656	2	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	3.91e-69	4	659	2	726	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
D7CFN7	5.59e-66	28	651	44	615	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000362	0.998859	0.000250	0.000168	0.000170	0.000169

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004876_00215.