CAZyme Information: MGYG000004828_01597

Basic Information

• Species: Fusobacterium_A sp900764925
• Lineage: Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium_A; Fusobacterium_A sp900764925
• CAZyme ID: MGYG000004828_01597
• CAZy Family: GH42
• CAZyme Description: Beta-galactosidase BgaA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
649	MGYG000004828_20|CGC1	75437.62	4.9062

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004828	2602027	MAG	China	Asia

• Gene Location: Start: 9692; End: 11641 Strand: +

Full Sequence      

MWLGVDYYPEQWDISMIDRDLDNIIELGSNVIRIGEFAWHLMEKEEGKFDFSFFDMVIKK
AEEKGLKVIFGTPTATIPAWLAKKYPEVLSEYENGQKRRFGGRHTSCYNSEKYIEYSKKI
VRALIEHYKDEKNIVAWQLDNEFGHEGSDECFCPCCEREFQNFLDKKFNGDINKLNETYG
TTFWSQEYNNFQEIPVPTSTITTHNPALRLDWERFRSESIVKYSDMQVDIIREIIPDAVI
IHDFPGGGLDKHVDYSKLAEKLDIVAYNNYPVWGGQKKPIPPYEIAFGLDYMRGLKRQNF
WITEGIMGAQGHDITGYLPRPNQAKMWSCQGVARGAESFIYFRYRGATKGAEQFCYGVID
ADNKKRRKFYEVQSFFNEVKANEASFETPIDSKVAMVYDYDSLASFRIQKQSILLDCHSE
MKRIHKVFYQKNIMIDIIPHTLDINKYEVIILPFMIIWKDEFVAKIKEFVSNGGKVIFTY
RNAVKDIDNNLTLGEMLPVRYGDLTGVYVEETESLQEYDELELKGVNEFEGVEGRAGIFR
DMLVPTTAETLIKYNDKFYNEFSAVTRNSFGAGEVYYIGCGLEDRLMEIVVDKVLESTSI
SGETTPEGVELVRRGHEDNKIKIYINHNDYPVKVKDFELKEFEFKITQE

Enzyme Prediction     

No EC number prediction in MGYG000004828_01597.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH42	6	380	2.7e-129	0.9946091644204852

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02449	Glyco_hydro_42	4.85e-140	6	381	1	375	Beta-galactosidase. This group of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. The enzyme catalyzes the hydrolysis of terminal, non-reducing terminal beta-D-galactosidase residues.
COG1874	GanA	1.68e-125	1	636	16	649	Beta-galactosidase GanA [Carbohydrate transport and metabolism].
pfam08532	Glyco_hydro_42M	7.66e-45	392	601	1	207	Beta-galactosidase trimerisation domain. This is non catalytic domain B of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. This domain is related to glutamine amidotransferase enzymes, but the catalytic residues are replaced by non functional amino acids. This domain is involved in trimerisation.
cd03143	A4_beta-galactosidase_middle_domain	3.11e-20	394	511	1	119	A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain. A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain. This group includes proteins similar to beta-galactosidase from Thermus thermophilus. Beta-Galactosidase hydrolyzes the beta-1,4-D-galactosidic linkage of lactose, as well as those of related chromogens, o-nitrophenyl-beta-D-galactopyranoside (ONP-Gal) and 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). This A4 beta-galactosidase middle domain lacks the catalytic triad of typical GATase1 domains. The reactive Cys residue found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow in typical GATase1 domains is not conserved in this group.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVQ18862.1	0.0	1	648	1	648
QNM14994.1	0.0	1	648	1	646
QQQ99503.1	7.17e-314	1	646	1	646
ANU45745.1	7.17e-314	1	646	1	646
QIX93296.1	6.79e-312	1	632	1	632

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3TTS_A	5.11e-100	1	628	9	636	ChainA, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_B Chain B, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_C Chain C, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_D Chain D, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_E Chain E, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_F Chain F, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_A Chain A, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_B Chain B, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_C Chain C, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_D Chain D, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_E Chain E, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_F Chain F, Beta-galactosidase [Niallia circulans subsp. alkalophilus]
6LVW_A	3.08e-96	1	615	1	629	PolyextremophilicBeta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi [Halorubrum lacusprofundi ATCC 49239]
1KWG_A	1.93e-86	3	615	2	604	Crystalstructure of Thermus thermophilus A4 beta-galactosidase [Thermus thermophilus],1KWK_A Crystal structure of Thermus thermophilus A4 beta-galactosidase in complex with galactose [Thermus thermophilus]
4OIF_A	1.34e-85	4	600	18	616	3Dstructure of Gan42B, a GH42 beta-galactosidase from G. [Geobacillus stearothermophilus],4OIF_B 3D structure of Gan42B, a GH42 beta-galactosidase from G. [Geobacillus stearothermophilus],4OIF_C 3D structure of Gan42B, a GH42 beta-galactosidase from G. [Geobacillus stearothermophilus]
4OJY_A	1.37e-85	4	600	19	617	3Dstructure of the E323A catalytic mutant of Gan42B, a GH42 beta-galactosidase from G. stearothermophilus [Geobacillus stearothermophilus],4OJY_B 3D structure of the E323A catalytic mutant of Gan42B, a GH42 beta-galactosidase from G. stearothermophilus [Geobacillus stearothermophilus],4OJY_C 3D structure of the E323A catalytic mutant of Gan42B, a GH42 beta-galactosidase from G. stearothermophilus [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D9SM34	1.16e-118	1	640	1	629	Beta-galactosidase BgaA OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=bgaA PE=1 SV=1
B9LW38	1.69e-95	1	615	1	629	Beta-galactosidase Bga OS=Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) OX=416348 GN=Hlac_2868 PE=1 SV=1
Q0TUR6	1.82e-95	4	641	17	665	Beta-galactosidase Pbg OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbg PE=3 SV=1
P94804	7.32e-95	1	614	1	612	Beta-galactosidase BgaH OS=Haloferax lucentense (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2) OX=1230452 GN=bgaH PE=1 SV=2
Q09HN2	4.08e-93	1	645	9	651	Beta-galactosidase BgaP OS=Planococcus sp. (strain L4) OX=377621 GN=bgaP PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004828_01597.