CAZyme Information: MGYG000004823_00562

Basic Information

• Species: CAG-462 sp900765575
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-462; CAG-462 sp900765575
• CAZyme ID: MGYG000004823_00562
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
803	MGYG000004823_22|CGC1	89881.03	7.0529

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004823	3872922	MAG	China	Asia

• Gene Location: Start: 12197; End: 14608 Strand: +

Full Sequence      

MKKVLLTLLGVIGASVQLVAQGVQGHGYPIKPVPFTSVKVTDAFWGQRLKASREVTIPLA
FSKCEETGRYENFYKAAHPSDSYKVEGYSFDDTDVYKTIEGASYILQTYPDKKLEQYIDS
VLTIVASAQEPDGYLYTARTMNPAHPHRWAGSKRWENVENLSHEFYNLGHMIEGAVAHYQ
ATGKRNFLDIAIRYADCVCREIGDGKGQIVAVPGHQIAEMALAKLYIVTGDRKYLDEAKF
FLDKRGYTANSRGEYSQAHKPVVEQDEAVGHAVRAAYMYSGMADVAALTGDSAYIRAIDK
IWDNIVSKKYYITGGIGATSKGEAFGQNYELPNMSAYCETCAAIGNVYLNHRLFLLHGES
KYYDVLERTLYNGLISGVSLDGGTFFYPNPLESVGQHQRQPWFGCACCPSNICRFIPSLP
GYVYAVKDKNVYVNLFMSNDAELKVNGKKLSLRQTTNYPFEGDTEIEVTKATGGDFTMKI
RIPGWVRNQPVPSDLYSYSDGKQSKYAVWVNGTPMEASVQADGYVTLSRKWRKGDKVAVH
FDMQPRIVKAHPNVAADRGRVAVEMGPLVYCAEWKDNDFDVLSVLMNKEPKFEGEQRSDV
LYGAMRLKTGAQVLNYDETGRLIVKDVQLTLIPYYAWAHRGAGAMAVWLPMELNACRPSQ
PETLASKSKLTASQPTAALSAINDRLVPQNADDRSMPYYHWWPKQGSTEWLVYTFPEATT
ISRSTVYWFDDEPWGGCRVPAAWKLYYKDEAGTWLPVAVAGTYGLNKGVGNEVSFTPICT
KELKLEVAQPEKYSCGLFEWEVK

Enzyme Prediction     

No EC number prediction in MGYG000004823_00562.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	38	571	4e-209	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	37	571	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	4.42e-178	29	649	2	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	0.002	179	315	99	243	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
COG1331	YyaL	0.003	169	301	414	563	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AII66185.1	0.0	1	803	1	801
QCD36182.1	0.0	1	803	1	802
QJR57394.1	0.0	1	803	1	801
QJR78977.1	0.0	1	803	1	801
AII67308.1	0.0	1	803	1	801

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	0.0	26	662	3	635	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	4.36e-148	36	649	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	9.11e-144	36	649	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.88e-106	37	648	2	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	3.84e-106	37	648	2	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.57e-105	37	648	2	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000912	0.998222	0.000265	0.000197	0.000180	0.000187

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004823_00562.