CAZyme Information: MGYG000004658_01322

Basic Information

• Species: Alistipes finegoldii
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; Alistipes finegoldii
• CAZyme ID: MGYG000004658_01322
• CAZy Family: GH5
• CAZyme Description: Endoglucanase E-5

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
333	MGYG000004658_57|CGC1	37900.61	6.237

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004658	3509081	Isolate	United States	North America

• Gene Location: Start: 11095; End: 12096 Strand: +

Full Sequence      

MKNYKLLSLIALTAAFSCSPNNEDGQSGNTPVEKYGALKVSGIQLCAQNGKALQLRGMST
HGLQWFGKCQTEEAYRSLAEEWQCDVVRLALYAEENGYNTDPLKFRNKIETLVGYCEKYG
MYCIIDWHVLHPGDPNDPKYDTADDFFAWASKKFAGKPHVLYEICNEPNGDEVTWAVVKR
YAERVIPIIRANSPNAVVICGTPKWSADFTDVVKDPLTYKNILYTYHFYAASHYNYDNLQ
NYLDRLPVFVTEWGTCEYNGSGKYDLDSSQRWIQIMDGDNPGKQRVSWATWNFSDGEGTA
DNLVPGSCAACRFTNVKQPYGQFIRDRIREKRP

Enzyme Prediction     

EC	3.2.1.4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	48	288	4.7e-85	0.9873417721518988

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	2.54e-58	46	295	1	270	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	7.74e-05	108	296	117	278	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFL77928.1	2.40e-263	1	333	1	333
AQG78093.1	4.69e-97	6	328	7	327
QYX37004.1	2.57e-92	10	330	31	357
QUX21074.1	2.57e-92	10	330	31	357
SCG17005.1	6.94e-91	28	329	173	477

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6GJF_A	4.67e-85	28	329	3	301	Ancestralendocellulase Cel5A [synthetic construct],6GJF_B Ancestral endocellulase Cel5A [synthetic construct],6GJF_C Ancestral endocellulase Cel5A [synthetic construct],6GJF_D Ancestral endocellulase Cel5A [synthetic construct],6GJF_E Ancestral endocellulase Cel5A [synthetic construct],6GJF_F Ancestral endocellulase Cel5A [synthetic construct]
2CKS_A	2.06e-84	30	310	3	284	X-RAYCRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5) [Thermobifida fusca],2CKS_B X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5) [Thermobifida fusca]
5WH8_A	2.49e-84	30	328	6	307	CellulaseCel5C_n [uncultured organism]
2CKR_A	5.84e-84	30	310	3	284	X-RAYCRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5) E355Q IN COMPLEX WITH CELLOTETRAOSE [Thermobifida fusca],2CKR_B X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5) E355Q IN COMPLEX WITH CELLOTETRAOSE [Thermobifida fusca]
4XZW_A	3.15e-74	30	307	4	280	Endo-glucanasechimera C10 [uncultured bacterium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q01786	6.27e-82	19	310	152	444	Endoglucanase E-5 OS=Thermobifida fusca OX=2021 GN=celE PE=1 SV=2
P27035	9.33e-73	30	328	151	450	Endoglucanase CelA OS=Streptomyces lividans OX=1916 GN=celA PE=1 SV=2
P06565	3.87e-72	32	330	32	328	Endoglucanase B OS=Evansella cellulosilytica (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4) OX=649639 GN=celB PE=3 SV=1
P07983	5.04e-72	9	329	13	330	Endoglucanase OS=Bacillus subtilis OX=1423 GN=bglC PE=3 SV=2
O85465	6.02e-72	1	330	1	328	Endoglucanase 5A OS=Salipaludibacillus agaradhaerens OX=76935 GN=cel5A PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000038	0.029314	0.970638	0.000010	0.000015	0.000012

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004658_01322.