CAZyme Information: MGYG000004655_00977

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873
• CAZyme ID: MGYG000004655_00977
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
677	MGYG000004655_13|CGC1	76976.14	5.7058

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004655	2984748	MAG	China	Asia

• Gene Location: Start: 31805; End: 33838 Strand: -

Full Sequence      

MTTLNRLIACTVCAIAMTGLSVKATGDTPTYTNNRYPLLTKPYMELPLGTIRPKGWLKEM
LVRQKNGASGRMDELYPEVMGPRNGWLGGDGDQWERGPYWIDGLLPLAYILDDKGLKKKV
QPWIEWVLASQRPDGYFGPDKDYQPEDGLQRDNSADWWPRMVLLKILQQYYSATGDIRVI
DFMTRYFHYQHATLPSRPLGHWTFWAEYRSGDNLQAVYWLYNITGDRTLLDLAETIHKQS
VDFTGMFTDSDKMTHFNTIHCVNLAQGLKEPVIYYQQSHDRKHVEAVKKGLCDITTYNGQ
PQGMYGGDEGLHGNVPTQGSELCSAVELMYSLEKMMEITGDTDFADRLERVAFNALPTQI
TDDFMAKQYFQQANQVMVTRQPHNFYEDHNHSHTDIVFGTLSGYPCCFSNMHQGWPKFTA
NLWFATHDNGLAALVYSPSTVTATVADGRTVTIDEETSYPMDGKITFSISLKDKKEPVSF
PLHLRVPGWCASPEVSINGTPAEATVSDGIIILDRTWKDGDSATLSLPMEIRVSRWHENS
AAIERGPLVYALRMDENWKKINLEGDDARQFGPYFYEVTSPSKWNYGIPEKALATPEEFF
KVHINHSKLNKSFFWNPESAPIEIKVKAKEVKEWSLYNGMAGPMPNSNTRSQAEEEEITL
IPYGCTTLRISEFPVLK

Enzyme Prediction     

No EC number prediction in MGYG000004655_00977.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	320	551	2.8e-38	0.4255725190839695

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	4.47e-68	99	551	62	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	6.54e-37	113	552	84	502	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMP98056.1	4.66e-160	31	676	28	651
ATP55412.1	4.79e-160	31	676	28	651
QNK64903.1	2.14e-157	31	676	22	645
ACU60445.1	1.46e-154	19	676	13	651
ALL07583.1	4.32e-154	31	676	28	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	5.02e-25	100	557	82	566	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	4.61e-23	100	557	82	566	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
5MQO_A	3.20e-16	90	562	100	620	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
3WRE_A	8.45e-15	317	557	334	572	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	8.58e-15	317	557	334	572	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	4.63e-14	317	557	334	572	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000253	0.999120	0.000189	0.000157	0.000146	0.000131

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004655_00977.