CAZyme Information: MGYG000004621_00321

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004621_00321
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
614	MGYG000004621_6|CGC1	69088.75	7.574

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004621	2838817	MAG	France	Europe

• Gene Location: Start: 28017; End: 29861 Strand: -

Full Sequence      

MKRMLILITPFLVSTALAQGQAVKAEKIRIDGWLGQKIGQCIRYQTMGEDVDQLTAPFYH
HEETGRWQTEFWGKWMLGAVGAYHYSPNPQLYNKMERSVRQMLCAQLPDGYIGNYSPGSQ
TMNWDIWGRKYTAMGLLAWYDLTGDKKTLASVRRLINHLMTQVGDGKADIVQCGIYKGMP
SCSVIIPVLRLYEFTRDPRYLAFARYIVARGESTEGPQLIGKADVPVALRWPHPANWYTK
ENGMKAYEMMSCYMGLLRLYHITHEESLLRAVETAVSHIMQEEINIAGSGSAFECWYGGN
HLQTLPAAHTMETCVTVTWMQLCAELLKVTKDTKYADEIERSAYNALIASMRADGQKIAK
YSPLAGHRYEGERQCGLDINCCTANAPRGFCLLPSVMYGGDNGEIDVNLYAPSTATVSID
GRHQVEMTQITGYPADGKVEIQVSPSRSHVFTVALRIPQWSRHTRVSVNGNDCGNITPGQ
YCRIHRTWTKGDRIVLNLDMQGQLQENSHCQAITYGPVVLARGSRFQDGFVDEGVDIKAN
NGTVALQRAEPADFAQLTFTVSTITGTNREGVRPRTIHLCDFASAGNDWDDRQRYRVWLP
KVIDVRTENVNEYK

Enzyme Prediction     

No EC number prediction in MGYG000004621_00321.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	72	520	3.5e-64	0.8893129770992366

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	5.29e-60	31	521	6	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	6.16e-59	56	599	52	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARE60508.1	4.60e-189	26	613	25	620
QHS51661.1	9.25e-46	74	538	123	587
QOY86860.1	2.24e-42	74	523	97	544
AXC10260.1	7.13e-41	74	523	133	593
AOZ94163.1	1.76e-40	21	523	7	561

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	3.92e-35	74	519	81	558	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	8.39e-32	74	519	81	558	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
5MQO_A	1.04e-26	74	523	105	602	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
7EXU_A	1.23e-14	74	523	80	561	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
3WRE_A	1.61e-14	74	523	80	561	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	8.79e-14	74	523	80	561	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000261	0.999056	0.000182	0.000179	0.000173	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004621_00321.