CAZyme Information: MGYG000004599_00072

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola
• CAZyme ID: MGYG000004599_00072
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
664	MGYG000004599_1|CGC3	75640.16	5.9704

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004599	3450206	MAG	France	Europe

• Gene Location: Start: 91123; End: 93117 Strand: +

Full Sequence      

MKKLLLLYSLLLSILSLQAERRFTLSSPNGNLSCSVFLGDTLSYSILFKGSQVLSKSSLY
MTLDNGETVGIYPKLRSCKRKSVDRIVESPFYRSSEISDKYNELVFNLKKEWSVEFRAYD
DGIAYRFVNGNDKEFKILDEHVEYSLSGDEKVIVPYVARGSDGDFESQFFNSFENTYTEC
NVSAINPGRLIFLPMMYETSNGIKVCITESDLENYPGLYLMGNSNCSLKGIHAPCPKRVE
QGGHNRLQMLVREREDFIASIDGKRSFPWRIAVVSDDACNIAASNLSYLLASPSRLSDIS
WVKPGKVAWDWWNDWNLDGVDFETGVNNDTYKAYIDFASKYGIEYVILDEGWAVNLEADL
MKVVKEIDLKSIIAYASSRNVGIILWAGYHAFNRDMENVCRHYSEMGIKGFKVDFMDRDD
QDMTSFNYRAAAMCAKYKLLLDLHGTSKPAGLNRTYPNVLNFEGVHGLEQMKWSSVNVDQ
VKYDVFLPFLRQLSGPMDYTQGAMRNATRTNYYPCNNEPMSQGTRCRQLALYIVFDSPFN
MLCDTPSNYMREQECTEFISSVPTFWDETKILKAEVGKCIVTARRKGNIWYIGGINDWNP
AELDIDLSFIEDVDNKSVEMFIDGINSHRIARDYRKSMLGRLGKSSIKVKLAPGGGFVMK
IFNK

Enzyme Prediction     

EC	3.2.1.22

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	13	661	8e-193	0.9920760697305864

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	9.81e-133	298	563	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	1.39e-67	27	293	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	6.40e-37	566	661	1	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQA09811.1	0.0	1	661	1	661
QUT72183.1	0.0	1	661	1	661
ALJ40715.1	0.0	1	661	1	661
QMW88818.1	0.0	1	661	1	661
AAO76978.1	0.0	1	661	1	661

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	0.0	27	661	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	0.0	20	661	13	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	1.94e-63	24	660	2	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	2.69e-63	24	660	2	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	7.09e-63	24	660	2	657	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	0.0	1	661	1	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	2.08e-53	15	661	15	723	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
D7CFN7	3.15e-46	26	594	44	561	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000201	0.999155	0.000175	0.000151	0.000139	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004599_00072.