CAZyme Information: MGYG000004474_01247

Basic Information

• Species: Sodaliphilus sp004557565
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Sodaliphilus; Sodaliphilus sp004557565
• CAZyme ID: MGYG000004474_01247
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
815	MGYG000004474_28|CGC2	91950.69	7.788

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004474	2861939	MAG	Israel	Asia

• Gene Location: Start: 20476; End: 22923 Strand: +

Full Sequence      

MGFGNMSNKGKKLSGNHSKTIIRVMWWSLACFMAFLLILSMLIYNGVIGYMPPVEELMHP
RDRYATTIYTSDGVEMGKFYRSKGNRSYVDFEQMSPHLKDALVATEDVRFEQHSGIDVRA
IFRSIIKRVILGKHSAGGGSTITQQLAKQLYSPESSGLFERALQKPIEWAIAIKIERFYT
KDEIVKMYFNQFDFLNNAVGISSASWVYFGKKPKDLNVQEAATLVGMCKNPSYYNPFRFP
ERVKERRNVVFDQMYKAGMLTSESCAALKATPLILNEHKVETHEDGIAPYFREELRRILM
ADEPDSTKYSNINAYYADKYNWDNNPVYGWCKKNKRKDGRNYDIYSDGLKIYTTVDSRMQ
QMAEAAVSEHMMEAQKRFFQNECGGKFTDPYTRNPDEMSKATKERVINMAIRSTERYRAM
KAEGKTHDEIMKAFDEKVEMRVFDYTAPDHEIVRVMSPRDSIMYMKTFLRCGMVAMDPRN
GKVKAYVGGPDFKHFKYDMVSTGTRQIGSTAKPFVFSLAMQNGFTPCSTDFENSTNGFGK
WQPKGGSHGLGLHPQLRDALAVSSNWVPPQILLRLGAPALVDALHNDFGITSKLDTTLTL
ALGSCEISLLEMVSAYAAFANYGQRPLPLMVSRICDNRGNVIAEFFPKLNQAISAESSYR
MVEMLRGVVTRGTGRRLGSYNFKGDVCGKTGTTNYNADAWWVGFTPEIVCGVWFGGEDRY
IHFQSTGEGQGAAAALPIFGKFLRKVYNSKELPYDENAKFIVPSDFKMCEDKIYDNEGGV
VYEHSGDGDGEDGDGPHPDPAVAAEDQSAVNDMFN

Enzyme Prediction     

No EC number prediction in MGYG000004474_01247.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	73	255	3.2e-59	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	2.67e-151	85	747	1	529	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	7.29e-144	19	763	3	730	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG0744	MrcB	5.56e-143	23	748	10	598	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	2.68e-71	32	744	3	782	penicillin-binding protein 1a; Provisional
TIGR02073	PBP_1c	3.79e-71	59	739	2	536	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASB36812.1	1.14e-277	12	774	2	768
ANU62694.1	1.14e-277	12	774	2	768
QQR10353.1	9.21e-276	25	774	1	754
QCP72948.1	2.24e-273	4	774	17	788
QCD39256.1	2.24e-273	4	774	17	788

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	6.15e-60	54	743	9	719	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.24e-59	68	743	23	712	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5FGZ_A	1.90e-39	98	743	168	690	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	2.14e-39	98	743	189	711	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3ZG8_B	1.15e-38	174	764	2	473	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O87626	6.25e-71	51	743	35	701	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
O87579	3.08e-70	51	744	35	703	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O66874	5.90e-69	77	771	52	697	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
O86088	1.92e-68	51	786	35	745	Penicillin-binding protein 1A OS=Neisseria cinerea OX=483 GN=mrcA PE=3 SV=1
P0A0Z5	4.97e-68	51	786	35	745	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000028	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
21	43