CAZyme Information: MGYG000004400_01298

Basic Information

• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-239; CAG-495
• CAZyme ID: MGYG000004400_01298
• CAZy Family: GH77
• CAZyme Description: 4-alpha-glucanotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
716	MGYG000004400_10|CGC1	82769.82	5.1617

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004400	1734828	MAG	Israel	Asia

• Gene Location: Start: 13831; End: 15981 Strand: +

Full Sequence      

MEKKLQELADKLGIATKFVDAGLIKKEYEIKDDIIRFFAGQLGCRAGTPEEIEHSLSSYE
KRRWQKSLEKIYVVEQAAVNFDLVIPAEFENEDFELRLKRQNTDVSPDVSFEVINHEEYQ
LVGKTKYVKLHFLITSPLEIGYYDVDLRVGSKRYRTVLAVTPKRCYVNPALTERKLWGFA
VQLYSVRSERNWGVGDFTDLAELVRICHRAGADVIGLNPLNVLSHDFPENASPYQSISRL
FLNPIYIDVEAVPEFTAADKTEVESEVYELRQSELIQYEHVYPLKVRLLEKMYERFKNGS
DVQRHAEYDDFCRKEGVELEKLAVFQCLYNEKAQTARGGWRSWEEEYRNQHSLAVKEYMH
THTDRIEFFKFMQFEAWRQFNQACHLVDELGLKVGFYRDLAVGVGQDSAELWSEPGLFFA
EAGAGAPPDAFFPCGQKWGLGAFDPNALKDDAYAPFIRILRANMHNAGALRIDHVMSLMR
LYIIPDKEETGTYVYYNLPDMLGIVALESALHRCSIVGESIGNVPDGFLDHLDNKNIYSL
SVLWAERKDSGWGDFNRPSEYPANAFVSVGTHDMAPLRMWWFGYDIEQAFQLGMIEDEQG
RTDAYKKREADRWKLLSALDESGVWPEDNLRKGNYLYGEAYPEGMEEAVHRFVARTPCPV
FLAQLEDILHVEKMQNLPGTDRDKHPNWRRKLPVNLERLESDIAYVRNIRAIKLER

Enzyme Prediction     

No EC number prediction in MGYG000004400_01298.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	181	712	1.5e-140	0.9858299595141701

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14507	PRK14507	0.0	1	716	4	721	malto-oligosyltrehalose synthase.
PRK11052	malQ	6.09e-180	1	716	2	689	4-alpha-glucanotransferase; Provisional
pfam02446	Glyco_hydro_77	1.75e-148	181	695	1	460	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
COG1640	MalQ	3.13e-135	165	716	1	516	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
PRK14510	PRK14510	5.41e-134	175	699	723	1201	bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKS54043.1	4.95e-144	5	702	4	714
CBS88424.1	5.31e-143	5	702	4	713
BAI73574.1	1.11e-140	5	702	4	720
AWU95656.1	1.41e-139	5	702	4	713
QCO14157.1	2.19e-138	5	716	4	728

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3P_A	3.96e-118	132	702	98	673	AmylomaltaseMalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3P_B Amylomaltase MalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3Q_A Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_B Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_C Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12]
4S3R_A	1.93e-114	132	702	98	673	AmylomaltaseMalQ from Escherichia coli in complex with the pseudo-heptasaccharide acarviosine-glucose-acarbose [Escherichia coli K-12]
5B68_A	6.29e-75	5	688	7	673	Crystalstructure of apo amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum],5JJH_A Crystal structure of amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum]
2X1I_A	2.58e-22	192	581	20	403	glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus]
1TZ7_A	6.30e-22	169	634	16	465	Aquifexaeolicus amylomaltase [Aquifex aeolicus],1TZ7_B Aquifex aeolicus amylomaltase [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P45176	7.82e-119	76	704	50	685	4-alpha-glucanotransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=malQ PE=3 SV=1
P15977	2.05e-117	132	702	98	673	4-alpha-glucanotransferase OS=Escherichia coli (strain K12) OX=83333 GN=malQ PE=1 SV=2
P9WK22	1.60e-81	5	689	8	684	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=malQ PE=3 SV=1
P65337	1.60e-81	5	689	8	684	4-alpha-glucanotransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=malQ PE=3 SV=1
P9WK23	1.60e-81	5	689	8	684	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=malQ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004400_01298.