CAZyme Information: MGYG000004392_00381

Basic Information

• Species: Eubacterium_R sp900539325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp900539325
• CAZyme ID: MGYG000004392_00381
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639		73429.15	6.25

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004392	1828862	MAG	Israel	Asia

• Gene Location: Start: 435085; End: 437004 Strand: -

Full Sequence      

MDKSLFSNHISLKNTNIDDEFWSKMQEKVRTKVIPYQYNALNDKIKNAEKSYCIDNFKKA
KKVIEAQKSGITTPTYPVDKWHYDENNADKNAFHGWVFQDSDAYKWLEAVAYSLNNKWDD
DLYTCACELIDLICSAQCDNGYLDTLYVINDQSKIFTNLKDFHELYCFGHLAEAAVAFYS
STKNEKLLTAALRFADLICDTFNENGKRGYPGHEIAELALVKLYKITGNEKYLKTAEFFI
NERGTKPYYFDIEQNIKTDGSSYVYNQAHMPIREQAEAVGHAVRGVYLYSGMADVAKETN
DEELYYACKRLWNNITTKKMYITGGIGSTVDGEAFSFDYDLPNDLAYCETCASIGLMFFG
MRMLEIAPNSQIADTMERALYNTIIAGMNDEGDRFFYVNPLEVLPEASKKDSRKRHIKTV
RQKWFGCACCPPNLARLISSIGEYCITENENTTYIHQFIGGKFKTNSADISITSSYTSNG
RVSIEVNPHKPFTLGIRIPAWCNNYNFSQGTNIKNDYAYFNITEHITINIKFDIEPKIIA
CSNLVRENIGKIAVTRGPFVYCLEEADNGKNLQMLRINTNLPLKIDNKKIIANGFRQKQN
DKLYFEFKTPCETETELTFIPYYKWGNRGENEMTVYIRY

Enzyme Prediction     

No EC number prediction in MGYG000004392_00381.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	16	562	9.2e-190	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.19e-158	12	638	9	586	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.79e-140	14	562	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	0.001	165	237	42	105	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].
cd04791	LanC_SerThrkinase	0.001	174	304	94	223	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
cd04792	LanM-like	0.006	179	247	605	695	Cyclases involved in the biosynthesis of class II lantibiotics, and similar proteins. LanM-like proteins. LanM is a bifunctional enzyme, involved in the synthesis of class II lantibiotics. It is responsible for both the dehydration and the cyclization of the precursor-peptide during lantibiotic synthesis. The C-terminal domain shows similarity to LanC, the cyclase component of the lan operon, but the N terminus seems to be unrelated to the dehydratase, LanB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU78348.2	1.46e-226	1	638	1	681
QQQ91696.1	1.46e-226	1	638	1	681
ASU31156.1	1.46e-226	1	638	1	681
QJU15845.1	2.06e-226	1	638	1	681
QBE95330.1	1.44e-222	1	638	1	675

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	4.45e-178	14	637	15	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	4.69e-172	14	637	15	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.31e-117	14	638	2	657	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.76e-117	14	638	2	657	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	4.92e-117	14	638	2	657	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	7.15e-117	14	638	2	657	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000037	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004392_00381.