CAZyme Information: MGYG000004369_00582

Basic Information

• Species: CAG-882 sp000435595
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-882; CAG-882 sp000435595
• CAZyme ID: MGYG000004369_00582
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
689		80248.68	5.0789

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004369	3090795	MAG	Israel	Asia

• Gene Location: Start: 29307; End: 31376 Strand: +

Full Sequence      

MRVWQEGNSLVIGDGRYTLRLETWGVNSLRVRMTGESVMDAHDWALCEKVEECVPEITCE
EVDTTDPWYKGDEYKKYHQTGKIYRMKNGKITAQISTEGWLSFYNQRGELLTEEYWRNRN
RINRYCVPIRVDARELKPIPGTTDYELTARFEAYDDEKIFGMGQYQEKHLDKKGAVLELA
HRNSQASVPFMISSRGYGFFWNNPAVGTASFGTNKTEWYAKSTKKLDYFITAGDTPFEIE
EQYSAAAGRTPMMPEYGLGYWQCKLRYRNQEELLAVAREHKRRGLPMDAIVVDFFHWTMQ
GEFKFEPRDWPDPEGMVKELKEMGIEPVVSVWPTIDERSENFGEMSDKGYLVTPDRGMAY
HMSWMGNTVFFDATNPGAQKFVWEKCRENYYRKGIRCFWLDEAEPEYGPYDFDNYRYYEG
PALQCTNTYPVGYAKGFYDGLKAEGEKDVMSLVRCAWAGSQKYGVLTWSGDIHSSFRAMR
EQLQAGLNMCVAGIPWWTSDIGGFVGGNIKDPYFKELLVRWFAWGCFCPVFRMHGERSPW
YEREEEFINNVRQLTSGQDNEVWSFGEDNYEILKKYLFIRERLRPYIRECMRHASLTGAP
VMRPLFFDFNDDKTAWNTEDEYMFGPDLLVAPVMVEKVVERSVYLPAGASWTDAYTGKVY
DGGQRVTVPAPLDVIPVFFKDGKKYDIYQ

Enzyme Prediction     

No EC number prediction in MGYG000004369_00582.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	228	683	9.2e-146	0.9929742388758782

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1501	YicI	1.72e-173	91	682	92	667	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
cd06591	GH31_xylosidase_XylS	2.26e-170	248	580	1	322	xylosidase XylS-like. XylS is a glycosyl hydrolase family 31 (GH31) alpha-xylosidase found in prokaryotes, eukaryotes, and archaea, that catalyzes the release of alpha-xylose from the non-reducing terminal side of the alpha-xyloside substrate. XylS has been characterized in Sulfolobus solfataricus where it hydrolyzes isoprimeverose, the p-nitrophenyl-beta derivative of isoprimeverose, and xyloglucan oligosaccharides, and has transxylosidic activity. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. The XylS family corresponds to subgroup 3 in the Ernst et al classification of GH31 enzymes.
pfam01055	Glyco_hydro_31	2.59e-152	228	682	1	439	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06603	GH31_GANC_GANAB_alpha	2.72e-69	248	683	1	424	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
PRK10658	PRK10658	4.06e-63	87	682	104	665	putative alpha-glucosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL08980.1	0.0	1	689	1	689
CBL11476.1	0.0	1	689	1	689
VCV22799.1	0.0	1	689	1	689
ADZ84377.1	0.0	1	689	1	689
QHQ59594.1	0.0	1	689	1	689

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6DRU_A	9.22e-118	86	678	76	704	Xylosidasefrom Aspergillus niger [Aspergillus niger],6DRU_B Xylosidase from Aspergillus niger [Aspergillus niger]
7KMP_A	1.26e-89	70	682	111	862	ChainA, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306],7KNC_A Chain A, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306]
5JOU_A	1.75e-83	215	682	364	841	Bacteroidesovatus Xyloglucan PUL GH31 [Bacteroides ovatus],5JOV_A Bacteroides ovatus Xyloglucan PUL GH31 with bound 5FIdoF [Bacteroides ovatus]
2XVG_A	1.63e-78	220	682	397	871	crystalstructure of alpha-xylosidase (GH31) from Cellvibrio japonicus [Cellvibrio japonicus],2XVK_A crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with 5-fluoro-alpha-D-xylopyranosyl fluoride [Cellvibrio japonicus],2XVL_A crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with Pentaerythritol propoxylate (5 4 PO OH) [Cellvibrio japonicus]
6JR6_A	1.44e-72	155	682	154	685	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR7_A Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q01336	4.38e-213	16	539	17	527	Uncharacterized family 31 glucosidase ORF2 (Fragment) OS=Pseudescherichia vulneris OX=566 PE=3 SV=1
Q9P999	1.86e-121	134	682	89	619	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1
A2QTU5	8.07e-117	86	678	94	722	Alpha-xylosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=axlA PE=1 SV=1
A7LXT0	9.42e-83	215	682	363	840	Alpha-xylosidase BoGH31A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02646 PE=1 SV=1
Q9F234	1.43e-66	157	682	147	671	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000074	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004369_00582.